DE69423361D1 - Geklonte pullulanase - Google Patents

Geklonte pullulanase

Info

Publication number
DE69423361D1
DE69423361D1 DE69423361T DE69423361T DE69423361D1 DE 69423361 D1 DE69423361 D1 DE 69423361D1 DE 69423361 T DE69423361 T DE 69423361T DE 69423361 T DE69423361 T DE 69423361T DE 69423361 D1 DE69423361 D1 DE 69423361D1
Authority
DE
Germany
Prior art keywords
pullulanase
cloned
cloned pullulanase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69423361T
Other languages
English (en)
Other versions
DE69423361T2 (de
Inventor
A Bower
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miller Brewing Co
Original Assignee
Miller Brewing Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miller Brewing Co filed Critical Miller Brewing Co
Application granted granted Critical
Publication of DE69423361D1 publication Critical patent/DE69423361D1/de
Publication of DE69423361T2 publication Critical patent/DE69423361T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8881—Modular construction, specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6047—Construction of the column with supporting means; Holders

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69423361T 1993-10-05 1994-10-04 Geklonte pullulanase Expired - Fee Related DE69423361T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13264893A 1993-10-05 1993-10-05
PCT/US1994/011242 WO1995009922A1 (en) 1993-10-05 1994-10-04 Cloned pullulanase

Publications (2)

Publication Number Publication Date
DE69423361D1 true DE69423361D1 (de) 2000-04-13
DE69423361T2 DE69423361T2 (de) 2000-12-07

Family

ID=22454966

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69423361T Expired - Fee Related DE69423361T2 (de) 1993-10-05 1994-10-04 Geklonte pullulanase

Country Status (7)

Country Link
US (1) US5514576A (de)
EP (1) EP0722501B1 (de)
JP (1) JP3007159B2 (de)
CA (1) CA2173453C (de)
DE (1) DE69423361T2 (de)
DK (1) DK0722501T3 (de)
WO (1) WO1995009922A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4447387A1 (de) 1994-12-22 1996-06-27 Inst Genbiologische Forschung Debranching-Enzyme aus Pflanzen und DNA-Sequenzen kodierend diese Enzyme
DE19608918A1 (de) * 1996-03-07 1997-09-11 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Mais codieren
DE19618125A1 (de) 1996-05-06 1997-11-13 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Kartoffel codieren
WO1998050562A1 (en) * 1997-05-06 1998-11-12 E.I. Du Pont De Nemours And Company Corn pullulanase
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
DE19836098A1 (de) 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
US6639126B1 (en) 1999-12-06 2003-10-28 Pioneer Hi-Bred International, Inc. Production of modified polysaccharides

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN150740B (de) * 1978-11-24 1982-12-04 Hoffmann La Roche
US4355110A (en) * 1979-07-19 1982-10-19 Miller Brewing Company Preparation of debranching enzyme from rice for producing a low calorie beer
US4355047A (en) * 1979-07-19 1982-10-19 Miller Brewing Company Method of preparing a low calorie beer
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
CA1211314A (en) * 1983-05-20 1986-09-16 William F. Line Production of dextrose and maltose syrups using an enzyme derived from rice
AU5912586A (en) * 1985-06-20 1986-12-24 Salk Institute Biotechnology/Industrial Associates, Inc., The Alpha-factor leader peptide-facilitated secretion from transformed s cerevisiae

Also Published As

Publication number Publication date
EP0722501B1 (de) 2000-03-08
CA2173453A1 (en) 1995-04-13
WO1995009922A1 (en) 1995-04-13
JPH09501842A (ja) 1997-02-25
JP3007159B2 (ja) 2000-02-07
DE69423361T2 (de) 2000-12-07
DK0722501T3 (da) 2000-08-21
EP0722501A1 (de) 1996-07-24
US5514576A (en) 1996-05-07
CA2173453C (en) 2001-02-13

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee