DE60138111D1 - Verfahren zur reinigung eines kalziumionen-bindenden proteins - Google Patents

Verfahren zur reinigung eines kalziumionen-bindenden proteins

Info

Publication number
DE60138111D1
DE60138111D1 DE60138111T DE60138111T DE60138111D1 DE 60138111 D1 DE60138111 D1 DE 60138111D1 DE 60138111 T DE60138111 T DE 60138111T DE 60138111 T DE60138111 T DE 60138111T DE 60138111 D1 DE60138111 D1 DE 60138111D1
Authority
DE
Germany
Prior art keywords
binding protein
purifying
calcium ion
protein
calcium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60138111T
Other languages
English (en)
Inventor
Hiroshi Mizokami
Shinichi Furukawa
Keishin Sugawara
Tatsufumi Onchi
Kazuhiro Komatsu
Satoshi Koyanagi
Hideo Yoshizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Kowa Co Ltd
Original Assignee
Chemo Sero Therapeutic Research Institute Kaketsuken
Kowa Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeutic Research Institute Kaketsuken, Kowa Co Ltd filed Critical Chemo Sero Therapeutic Research Institute Kaketsuken
Application granted granted Critical
Publication of DE60138111D1 publication Critical patent/DE60138111D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Removal Of Specific Substances (AREA)
DE60138111T 2000-07-21 2001-07-18 Verfahren zur reinigung eines kalziumionen-bindenden proteins Expired - Lifetime DE60138111D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000220600 2000-07-21
PCT/JP2001/006209 WO2002008249A1 (fr) 2000-07-21 2001-07-18 Procede de purification de la proteine de liaison a l'ion calcium

Publications (1)

Publication Number Publication Date
DE60138111D1 true DE60138111D1 (de) 2009-05-07

Family

ID=18715177

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60138111T Expired - Lifetime DE60138111D1 (de) 2000-07-21 2001-07-18 Verfahren zur reinigung eines kalziumionen-bindenden proteins

Country Status (9)

Country Link
US (2) US6869934B2 (de)
EP (1) EP1306383B1 (de)
KR (1) KR100841599B1 (de)
CN (1) CN1250567C (de)
AT (1) ATE426611T1 (de)
AU (1) AU2001272736A1 (de)
CA (1) CA2385371A1 (de)
DE (1) DE60138111D1 (de)
WO (1) WO2002008249A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011247567B2 (en) 2010-04-29 2014-06-19 Takeda Pharmaceutical Company Limited Purification method for divalent cation binding proteins on anion exchange resin
CN102382168A (zh) * 2010-09-03 2012-03-21 中国科学院过程工程研究所 一种高效纯化金属离子结合蛋白的离子交换层析分离纯化方法
LT2748179T (lt) 2011-10-14 2022-04-11 Takeda Pharmaceutical Company Limited Baltymų gryninimas anijonų mainų chromatografija
ES2700933T3 (es) * 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
US10202417B2 (en) 2013-03-15 2019-02-12 Baxalta Incorporated Purification method for vitamin K dependent proteins by anion exchange chromatography
US11904082B2 (en) 2016-08-01 2024-02-20 Kaneka Corporation Adsorbent for calciprotein particles, adsorption removal system, and method for utilization thereof

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1265446A (en) * 1985-09-30 1990-02-06 Masahiro Maki Anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component
JP2660514B2 (ja) * 1987-02-20 1997-10-08 興和株式会社 抗血液凝固作用を有するポリペプチド
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DK0409053T3 (da) * 1989-07-15 1993-11-08 Boehringer Ingelheim Int Fremgangsmåde til rensning af annexiner
JP2743207B2 (ja) 1990-01-25 1998-04-22 財団法人化学及血清療法研究所 Ｃｐｂ―ｉの製造方法並びにこれに用いる組換えプラスミドおよび形質転換酵母
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
WO1996003655A1 (en) * 1994-07-23 1996-02-08 Boehringer Mannheim Gmbh Method for the determination of the prethrombotic state
EP0731166A3 (de) * 1995-03-06 1997-12-29 Tonen Corporation Neue Calcium-bindende Proteine
US5976832A (en) * 1995-03-06 1999-11-02 Tonen Corporation DNA encoding novel calcium-binding proteins
US5658877A (en) * 1995-05-18 1997-08-19 Wisconsin Alumni Research Foundation Method to treat endotoxin effects by administration of 33 kilodalton phospholipid binding protein
US6645465B2 (en) * 1999-08-06 2003-11-11 Michigan, University Of The Regents Annexin proteins and autoantibodies as serum markers for cancer

Also Published As

Publication number Publication date
WO2002008249A1 (fr) 2002-01-31
ATE426611T1 (de) 2009-04-15
CA2385371A1 (en) 2002-01-31
US6869934B2 (en) 2005-03-22
US20050059805A1 (en) 2005-03-17
EP1306383A4 (de) 2005-01-26
CN1392878A (zh) 2003-01-22
KR20020057960A (ko) 2002-07-12
CN1250567C (zh) 2006-04-12
KR100841599B1 (ko) 2008-06-26
EP1306383A1 (de) 2003-05-02
US20020169294A1 (en) 2002-11-14
AU2001272736A1 (en) 2002-02-05
EP1306383B1 (de) 2009-03-25

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