CN220867447U - Regulation and control macrophage culture device - Google Patents
Regulation and control macrophage culture device Download PDFInfo
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- CN220867447U CN220867447U CN202322627421.4U CN202322627421U CN220867447U CN 220867447 U CN220867447 U CN 220867447U CN 202322627421 U CN202322627421 U CN 202322627421U CN 220867447 U CN220867447 U CN 220867447U
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- cell culture
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- bottle
- macrophage
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- 210000002540 macrophage Anatomy 0.000 title claims abstract description 43
- 230000033228 biological regulation Effects 0.000 title claims description 4
- 238000004113 cell culture Methods 0.000 claims abstract description 92
- 230000001105 regulatory effect Effects 0.000 claims abstract description 22
- 230000001276 controlling effect Effects 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims description 18
- 210000000481 breast Anatomy 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 239000012531 culture fluid Substances 0.000 claims 1
- 229930003316 Vitamin D Natural products 0.000 abstract description 16
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 abstract description 16
- 235000019166 vitamin D Nutrition 0.000 abstract description 16
- 239000011710 vitamin D Substances 0.000 abstract description 16
- 150000003710 vitamin D derivatives Chemical class 0.000 abstract description 16
- 229940046008 vitamin d Drugs 0.000 abstract description 16
- 206010028980 Neoplasm Diseases 0.000 abstract description 7
- 210000004881 tumor cell Anatomy 0.000 abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 6
- 239000008103 glucose Substances 0.000 abstract description 6
- 210000002865 immune cell Anatomy 0.000 abstract description 5
- 230000010287 polarization Effects 0.000 abstract description 5
- 230000005809 anti-tumor immunity Effects 0.000 abstract description 3
- 230000003013 cytotoxicity Effects 0.000 abstract description 3
- 231100000135 cytotoxicity Toxicity 0.000 abstract description 3
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 21
- 238000012258 culturing Methods 0.000 description 14
- 210000001519 tissue Anatomy 0.000 description 3
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000034964 establishment of cell polarity Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a regulating and controlling macrophage culture device, which relates to the technical field of cell culture and comprises a first cell culture bottle, wherein a connecting column is fixedly connected to the left side of the outer surface of the first cell culture bottle, a regulating valve is connected to the center of the upper side of the outer surface of the connecting column in a threaded manner, and a second cell culture bottle is fixedly connected to the left side of the outer surface of the connecting column. Compared with the existing cell culture device commonly used for macrophage culture, the device has the advantages that active vitamin D is placed in the first cell culture bottle, the active vitamin D is poured into the culture solution to enable the cell culture bottle to be beyond a connecting column, then the macrophage and tumor cells are placed in the second cell culture bottle, the culture solution and glucose are poured into the second cell culture bottle, then the active vitamin D is controlled to reach the first cell culture bottle through the regulating valve, the active vitamin D promotes the polarization of the macrophage, and the polarized macrophage has an inhibiting effect on the cytotoxicity function of immune cells killing tumors, so that the antitumor immunity is weakened.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a device for regulating and controlling macrophage culture.
Background
Macrophages are white blood cells that reside within tissue and are derived from monocytes, which in turn are derived from precursor cells in the bone marrow. Macrophages and monocytes are phagocytes and participate in nonspecific and specific defense in vertebrates. Their main functions are to take the form of fixed or free cells, to take the effect of phagocytosis of cell debris and pathogens, and to activate lymphocytes or other immune cells to react to pathogens. Macrophages belong to immune cells and have multiple functions, and are important subjects for studying cytophagy, cellular immunity and molecular immunology. Macrophages are readily available, convenient to culture, and can be purified. The macrophage does not reproduce cell population, can live for 2-3 weeks under proper conditions, is mostly used as primary culture, is difficult to survive for a long time, and the cell culture device provides a proper growth environment for cells so that the cells can be rapidly proliferated and form a needed biological tissue product. Because animal cells are different from microbial cells in terms of morphological structure, culture method, required mechanical environment and the like, the traditional microbial reactor is obviously not suitable for large-scale culture of animal cells, especially for the requirement of tissue engineering, and research and development of a novel bioreactor are promoted. The existing cell culture device for macrophage culture is often complicated to operate, and meanwhile, the device is often difficult to operate conveniently, so that the cell culture is influenced.
The utility model discloses a cell culture device for macrophage culture, which has the advantages that an upper cover is rotated to drive a limit column to rotate around a fixed rod in the middle of the limit column, an ear seat is used for enabling the fixed rod to be fixed at a specified position, a worker can conveniently open or close the upper cover, the worker can conveniently observe the internal condition of a temperature control box under the condition that cell culture in the temperature control box is not affected by the glass cover, a plurality of supporting legs play a supporting role on the temperature control box, and the temperature and humidity in the temperature control box body are conducted to a control panel through a temperature and humidity sensor.
Accordingly, in view of the above, an apparatus for controlling macrophage culture is proposed to solve the problems of the prior art.
Disclosure of utility model
The present utility model is directed to a device for controlling macrophage culture, which solves the problems set forth in the background art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a regulation and control macrophage culture device, includes cell culture bottle one, cell culture bottle one's surface left side fixedly connected with spliced pole, the surface upside center department threaded connection of spliced pole has the governing valve, the lower fixed surface of governing valve is connected with the block, the internal surface center department fixedly connected with breast board of spliced pole, the block groove has been seted up to the surface left and right sides of breast board, the surface left side fixedly connected with cell culture bottle two of spliced pole.
Preferably, a culture solution supply pipe I is fixedly connected to the right side of the upper surface of the cell culture bottle I, and a feeding device I is fixedly connected to the center of the upper surface of the cell culture bottle I.
Preferably, the upper surface of the first feeding device is provided with ventilation holes, and the left side of the upper surface of the first cell culture bottle is fixedly connected with an active gas supply pipe.
Preferably, the left side of the lower surface of the active gas supply pipe is fixedly connected with a second cell culture bottle, the center of the upper surface of the second cell culture bottle is fixedly connected with a second feeding device, and the left side of the upper surface of the second cell culture bottle is fixedly connected with a second culture solution supply pipe.
Preferably, the lower surface of the first cell culture bottle is fixedly connected with a bottom plate, and the upper surface of the bottom plate is fixedly connected with a support column.
Preferably, the upper surface of the pillar is fixedly connected with a heating device, and the inner surface of the heating device is fixedly connected with a heating plate.
Preferably, the lower surface block of cell culture bottle two is connected with the spacing ring, cell culture bottle two's surface fixedly connected with sampling device.
Compared with the prior art, the utility model has the beneficial effects that:
1. According to the utility model, through the arrangement of the first cell culture bottle, the connecting column, the regulating valve, the clamping block, the baffle plate, the clamping groove and the second cell culture bottle, researches show that active vitamin D promotes macrophage polarization through a VDR-PPARgamma channel in a high-sugar environment, so that the active vitamin D is put into the first cell culture bottle, the culture solution is poured into the first cell culture bottle so as to overflow the connecting column, then macrophages and tumor cells are put into the second cell culture bottle, the culture solution and glucose are poured into the second cell culture bottle, the culture solution and the glucose do not reach the connecting column, finally, the space between the clamping block and the clamping groove on the baffle plate is enlarged through the regulating valve so as to control the active vitamin D to reach the second cell culture bottle, the active vitamin D is polarized, and the polarized macrophages play a role in inhibiting the cytotoxicity function of killing immune cells of tumors, so that the antitumor immunity is weakened, and the ecological position of immunosuppressive tumors is facilitated;
2. According to the utility model, through the arrangement of the first cell culture bottle, the second cell culture bottle, the bottom plate, the heating device and the heating plate, proper temperature is required to be given when the macrophage is cultured, the heating device is arranged on the bottom plate and is looped on the periphery of the first cell culture bottle and the second cell culture bottle, and proper growth environment is provided for the macrophages when the cell is cultured;
3. According to the utility model, through the arrangement of the limiting ring, the cell culture bottle II and the cell culture bottle I and the bottom plate, after the macrophage culture experiment is finished, the cell culture bottle I and the cell culture bottle II are taken down from the limiting ring on the bottom plate for cleaning, and then the cleaned cell culture bottle I and the cell culture bottle II are placed in the limiting ring, and the limiting ring can protect the cell culture bottle I and the cell culture bottle II from shaking and dumping due to stress.
Drawings
FIG. 1 is a schematic view of the overall three-dimensional structure of the present utility model;
FIG. 2 is an enlarged schematic view of the structure of FIG. 1A according to the present utility model;
FIG. 3 is a schematic view of the overall perspective structure of the heating device of the present utility model;
Fig. 4 is a schematic overall perspective view of the valve in the connecting column of the present utility model.
In the figure: 1. a first cell culture bottle; 2. a second cell culture bottle; 3. a connecting column; 4. a regulating valve; 5. a breast board; 6. a clamping block; 7. a clamping groove; 8. a limiting ring; 9. a bottom plate; 10. a support post; 11. a heating plate; 12. a heating device; 13. a sampling device; 14. a first culture liquid supply pipe; 15. a second culture solution supply pipe; 16. a first feeding device; 17. a second feeding device; 18. an active gas supply pipe; 19. and (5) ventilation holes.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in figures 1-4, the device for regulating and controlling macrophage culture comprises a first cell culture bottle 1, a connecting column 3 is fixedly connected to the left side of the outer surface of the first cell culture bottle 1, a regulating valve 4 is connected to the center of the upper side of the outer surface of the connecting column 3 in a threaded manner, a clamping block 6 is fixedly connected to the lower surface of the regulating valve 4, a baffle 5 is fixedly connected to the center of the inner surface of the connecting column 3, a clamping groove 7 is formed in the left side and the right side of the outer surface of the baffle 5, a second cell culture bottle 2 is fixedly connected to the left side of the outer surface of the connecting column 3, research shows that active vitamin D in a high-sugar environment promotes macrophage polarization through a VDR-PPAR gamma channel, so that active vitamin D is put into the first cell culture bottle 1 to be poured into the connecting column 3, then macrophages and tumor cells are put into the second cell culture bottle 2, the culture liquid and glucose are poured into the connecting column 3, and finally the space between the clamping block 6 and the baffle 5 is enlarged through the regulating valve 4 to control the active vitamin D to reach the second cell culture bottle 2, thus the active vitamin D is controlled to reach the second cell culture bottle 2, the immunological competence is inhibited, the immunological competence is further weakened, and the tumor polarization is further inhibited, and the tumor cell polarization is further inhibited, and the immunity against tumor cell polarization is further has been inhibited.
As shown in fig. 1, a first culture solution supply pipe 14 is fixedly connected to the right side of the upper surface of a first cell culture bottle 1, a first feeding device 16 is fixedly connected to the center of the upper surface of the first cell culture bottle 1, an air hole 19 is formed in the upper surface of the first feeding device 16, an active gas supply pipe 18 is fixedly connected to the left side of the upper surface of the first cell culture bottle 1, a second cell culture bottle 2 is fixedly connected to the left side of the lower surface of the active gas supply pipe 18, a second feeding device 17 is fixedly connected to the center of the upper surface of the second cell culture bottle 2, a second culture solution supply pipe 15 is fixedly connected to the left side of the upper surface of the second cell culture bottle 2, active vitamin D and culture solution are placed into the first cell culture bottle 1 through the first feeding device 16, air in the first cell culture bottle 1 is communicated with the outside through the air hole 19, then the culture solution is required to be poured into the first cell culture bottle 14, macrophages, tumor cells and glucose are placed into the second cell culture bottle 2 through the second feeding device 17, air in the second cell culture bottle 2 is communicated with the outside through the air hole 19, and the active gas supply pipe 18 and the active cells are given to the first cell culture bottle 1.
As shown in fig. 1-3, the bottom plate 9 is fixedly connected to the lower surface of the first cell culture flask 1, the supporting column 10 is fixedly connected to the upper surface of the bottom plate 9, the heating device 12 is fixedly connected to the upper surface of the supporting column 10, the heating plate 11 is fixedly connected to the inner surface of the heating device 12, a proper temperature is required to be given when the macrophage is cultured, the heating device 12 is arranged on the bottom plate 9, the heating device 12 is looped around the periphery of the first cell culture flask 1 and the second cell culture flask 2, and a proper growth environment is given to the macrophages when the cell culture is performed.
As shown in fig. 1-2, the lower surface of the second cell culture bottle 2 is clamped and connected with a limiting ring 8, the outer surface of the second cell culture bottle 2 is fixedly connected with a sampling device 13, after the macrophage culture experiment is completed, the first cell culture bottle 1 and the second cell culture bottle 2 are taken down from the limiting ring 8 on the bottom plate 9 to be cleaned, then the cleaned first cell culture bottle 1 and the cleaned second cell culture bottle 2 are placed in the limiting ring 8, and the limiting ring 8 can protect the first cell culture bottle 1 and the second cell culture bottle 2 from shaking and dumping due to stress.
Working principle: when the regulating and controlling macrophage culturing device is used, firstly, the first cell culturing bottle 1 and the second cell culturing bottle 2 are taken down from the limiting ring 8 on the bottom plate 9 for disinfection and cleaning, then the first cell culturing bottle 1 and the second cell culturing bottle 2 are reset, then macrophages and tumor cells are put into the second cell culturing bottle 2, then the culture solution and glucose are poured into the second cell culturing bottle 2, the liquid in the second cell culturing bottle 2 does not reach the position of the connecting column 3, then the culture solution and active vitamin D are put into the first cell culturing bottle 1, the culture solution and the active vitamin D do not pass through the connecting column 3, then the heating device 12 is started to give the proper temperature in the first cell culturing bottle 1 and the second cell culturing bottle 2, then the liquid in the first cell culturing bottle 1 is regulated by the regulating valve 4 to flow into the second cell culturing bottle 2, the active vitamin D in the high sugar environment promotes the polarization of the macrophages through the VDR-gamma channel, the polarized macrophages generate the inhibiting effect on the cytotoxicity function of the immune cells killing tumors, thus the antitumor immunity is weakened, the ecological position of the immune tumor is helped, then the tumor is observed, and then the sample is taken out by the sampling device 13, the sample is taken out, the regulating and controlling the operating principle of the macrophages is the regulating and controlling the culturing device.
The examples of the present utility model are presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the utility model in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments were chosen and described in order to best explain the principles of the utility model and the practical application, and to enable others of ordinary skill in the art to understand the utility model for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (7)
1. The utility model provides a regulation and control macrophage culture device, includes cell culture bottle one (1), its characterized in that, surface left side fixedly connected with spliced pole (3) of cell culture bottle one (1), surface upside center department threaded connection of spliced pole (3) has governing valve (4), the lower fixed surface of governing valve (4) is connected with block (6), the internal surface center department fixedly connected with breast board (5) of spliced pole (3), block groove (7) have been seted up on the surface left and right sides of breast board (5), surface left side fixedly connected with cell culture bottle two (2) of spliced pole (3).
2. The device for regulating and controlling macrophage culture according to claim 1, wherein a culture fluid supply pipe I (14) is fixedly connected to the right side of the upper surface of the cell culture bottle I (1), and a feeding device I (16) is fixedly connected to the center of the upper surface of the cell culture bottle I (1).
3. The device for regulating and controlling macrophage culture according to claim 2, wherein the upper surface of the first feeding device (16) is provided with an air hole (19), and the left side of the upper surface of the first cell culture flask (1) is fixedly connected with an active gas supply pipe (18).
4. A device for regulating and controlling macrophage culture according to claim 3, wherein the left side of the lower surface of the active gas supply pipe (18) is fixedly connected with a second cell culture bottle (2), the center of the upper surface of the second cell culture bottle (2) is fixedly connected with a second feeding device (17), and the left side of the upper surface of the second cell culture bottle (2) is fixedly connected with a second culture liquid supply pipe (15).
5. The device for regulating and controlling macrophage culture according to claim 1, wherein a bottom plate (9) is fixedly connected to the lower surface of the first cell culture flask (1), and a support column (10) is fixedly connected to the upper surface of the bottom plate (9).
6. The device for regulating and controlling macrophage culture according to claim 5, wherein the upper surface of the pillar (10) is fixedly connected with a heating device (12), and the inner surface of the heating device (12) is fixedly connected with a heating plate (11).
7. The device for regulating and controlling macrophage culture according to claim 1, wherein a limiting ring (8) is connected to the lower surface of the second cell culture flask (2) in a clamping manner, and a sampling device (13) is fixedly connected to the outer surface of the second cell culture flask (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322627421.4U CN220867447U (en) | 2023-09-25 | 2023-09-25 | Regulation and control macrophage culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322627421.4U CN220867447U (en) | 2023-09-25 | 2023-09-25 | Regulation and control macrophage culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220867447U true CN220867447U (en) | 2024-04-30 |
Family
ID=90822291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322627421.4U Active CN220867447U (en) | 2023-09-25 | 2023-09-25 | Regulation and control macrophage culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220867447U (en) |
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2023
- 2023-09-25 CN CN202322627421.4U patent/CN220867447U/en active Active
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