CN220120704U - Protein detection device - Google Patents
Protein detection device Download PDFInfo
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- CN220120704U CN220120704U CN202321607360.9U CN202321607360U CN220120704U CN 220120704 U CN220120704 U CN 220120704U CN 202321607360 U CN202321607360 U CN 202321607360U CN 220120704 U CN220120704 U CN 220120704U
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- light source
- detection chamber
- monochromator
- sample detection
- sample
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- 238000002331 protein detection Methods 0.000 title claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 60
- 238000005286 illumination Methods 0.000 claims abstract description 19
- 238000012545 processing Methods 0.000 claims abstract description 9
- 230000003321 amplification Effects 0.000 claims abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 64
- 239000010453 quartz Substances 0.000 claims description 19
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 230000003287 optical effect Effects 0.000 claims description 11
- 229910052805 deuterium Inorganic materials 0.000 claims description 9
- 229910052721 tungsten Inorganic materials 0.000 claims description 9
- 239000010937 tungsten Substances 0.000 claims description 9
- 229910052724 xenon Inorganic materials 0.000 claims description 9
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 9
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 claims description 8
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 8
- 230000005855 radiation Effects 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000005693 optoelectronics Effects 0.000 claims description 2
- 239000012488 sample solution Substances 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 230000017525 heat dissipation Effects 0.000 description 5
- 239000002131 composite material Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses a protein detection device, which comprises a shell, wherein a sample detection chamber is arranged in the shell, the sample detection chamber is connected with a processing display assembly through a signal amplification assembly, the sample detection chamber is connected with a temperature control assembly for adjusting the temperature of a sample, one side of the sample detection chamber is respectively provided with a first light source assembly and a second light source assembly, a first monochromator is arranged between the first light source assembly and the sample detection chamber, and a second monochromator is arranged between the second light source assembly and the sample detection chamber; according to the utility model, the first light source component and the second light source component are used for illuminating the solution sample in the sample detection chamber, parallel monochromatic light is provided for the solution sample in the sample detection chamber through the combination of the first light source component and the first monochromator, the illumination intensity and the wavelength are respectively adjusted through adjusting the first light source component and the first monochromator, and meanwhile, the temperature control component is used for keeping the environment of the solution sample in the sample detection chamber at a proper temperature.
Description
Technical Field
The utility model relates to the technical field of protein detection, in particular to a protein detection device.
Background
Protein measurement refers to measurement of protein content by physical or chemical methods, and protein is an important component constituting human cells and tissues.
According to Beer-Lambert law, a sample is added into a detection pool, firstly, wavelength scanning of 200-800nm is carried out on an ultraviolet-visible light spectrophotometer, then, the cuvette is taken out of the instrument, a certain time is irradiated under an LED lamp with a certain wavelength, the quartz cuvette is put into the spectrophotometer again, absorption spectrum scanning is carried out, repeated times are carried out, the activation characteristic of light-sensitive proteins is analyzed, then, the light intensity irradiance meter is utilized to measure the illumination intensity of different illumination time, and the illumination dynamics analysis is carried out through calculation.
Although the prior art has realized detection of protein sample concentration to some extent, the following problems still exist to be solved:
firstly, in the prior art experiment, an LED illumination device and a spectrophotometer device are independent elements, protein samples contained in a quartz cuvette need to be repeatedly carried in two instruments, the quartz cuvette containing the protein samples is a fragile product, and the situation that the protein samples collide with and break up in the transferring process easily occurs, so that the experimental samples are lost.
Secondly, the wavelength of the LED illumination device in the prior art is composite light, the incident light needs to be parallel monochromatic light according to the application condition of Beer-Lambert law, and the composite light of the LED illumination device has stray light outside the wavelength required by experiments and has unadjustable wavelength, so that the light intensity fluctuation is large, and the accuracy of a detection result is influenced.
Thirdly, the LED illuminating device in the prior art can generate a large amount of heat, protein in a sample solution can be influenced at high temperature, the LED illuminating device is placed at room temperature and is easily influenced by environmental factors such as seasons, weather and the like, the sample is interfered by the temperature, the condition that an object to be detected is a uniform dilute solution in the application condition of Beer-Lambert law cannot be well met, and therefore accuracy of scientific experiments is influenced.
Disclosure of Invention
The utility model aims to provide a protein detection device, which solves the technical problem that the influence of LEDs on experimental results in the existing protein detection scheme is still not solved in the prior art, and specifically provides the following technical scheme:
the protein detection device comprises a shell, wherein a sample detection chamber is arranged in the shell, the sample detection chamber is connected with a processing display assembly through a signal amplification assembly, the processing display assembly is arranged on one side of the shell, the sample detection chamber is connected with a temperature control assembly for adjusting the temperature of a sample, a first light source assembly and a second light source assembly are respectively arranged on one side of the sample detection chamber, a first monochromator is arranged between the first light source assembly and the sample detection chamber, and a second monochromator is arranged between the second light source assembly and the sample detection chamber;
the straight line where the first light source component and the first monochromator are located is perpendicular to the straight line where the second light source component and the second monochromator are located, and the sample detection chamber is arranged at the intersection point of the two straight lines.
Further, the sample detection chamber comprises a quartz cuvette frame used for placing a quartz cuvette, openings used for illumination transmission are formed in the periphery of the quartz cuvette frame, a detector is arranged on one side of the quartz cuvette frame along the linear extending direction of the second light source component and the second monochromator, and the detector is electrically connected with the signal amplifying component;
when the detector detects light from the second light source assembly, an optoelectronic signal is transmitted to the signal amplifying assembly by the detector.
Further, the first light source component comprises a first spherical mirror arranged on the inner side of the shell, a xenon lamp is arranged on the mirror surface side of the first spherical mirror, and the center of the mirror surface side of the first spherical mirror is opposite to the sample placing position of the sample detection chamber;
the xenon lamp is connected with a rheostat, and a light intensity probe for detecting illumination intensity is arranged on one side of the quartz cuvette frame along the linear extending direction of the first light source component and the first monochromator.
Further, the first monochromator comprises a first optical filter, a first light slit and a first grating which are sequentially arranged on one side of the first spherical mirror, and a plurality of mirrors for ensuring monochromatic light to be reflected into the sample detection chamber are further arranged in the first monochromator.
Further, the second light source assembly comprises a second spherical mirror arranged on the inner side of the shell, a deuterium lamp and a tungsten lamp are respectively arranged on the mirror surface side of the second spherical mirror, and the deuterium lamp and the tungsten lamp are both connected with a control and regulation plate for regulating the wavelength.
Further, the second monochromator comprises a second optical filter, a second light slit and a second grating which are sequentially arranged on one side of the second spherical mirror, and a plurality of mirrors for ensuring monochromatic light to be reflected into the sample detection chamber are further arranged in the second monochromator.
Compared with the prior art, the utility model has the following beneficial effects:
according to the utility model, the first light source component and the second light source component are used for illuminating the solution sample in the sample detection chamber, parallel monochromatic light is provided for the solution sample in the sample detection chamber through the combination of the first light source component and the first monochromator, the illumination intensity and the wavelength are respectively adjusted through adjusting the first light source component and the first monochromator, and meanwhile, the temperature control component is used for keeping the environment of the solution sample in the sample detection chamber at a proper temperature.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those of ordinary skill in the art that the drawings in the following description are exemplary only and that other implementations can be obtained from the extensions of the drawings provided without inventive effort.
Fig. 1 is a schematic top view of the present utility model.
Reference numerals in the drawings are respectively as follows:
1-shell, 2-sample detection chamber, 3-signal amplifying component, 4-processing display component, 5-temperature control component, 6-first light source component, 7-first monochromator, 8-second light source component and 9-second monochromator;
11-heat radiation opening, 12-heat radiation fan
21-quartz cuvette frame, 22-detector and 23-light intensity probe;
61-a first spherical mirror, 62-a xenon lamp and 63-a rheostat;
71-a first optical filter, 72-a first light slit and 73-a first grating;
81-second spherical mirror, 82-deuterium lamp and 83-tungsten lamp
91-second filter, 92-second light slit, 93-second grating.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1, the utility model provides a protein detection device, which comprises a shell 1, wherein a sample detection chamber 2 is arranged in the shell 1, the sample detection chamber 2 is connected with a processing display assembly 4 through a signal amplification assembly 3, the processing display assembly 4 is arranged on one side of the shell 1, the sample detection chamber 2 is connected with a temperature control assembly 5 for adjusting the temperature of a sample, one side of the sample detection chamber 2 is respectively provided with a first light source assembly 6 and a second light source assembly 8, a first monochromator 7 is arranged between the first light source assembly 6 and the sample detection chamber 2, and a second monochromator 9 is arranged between the second light source assembly 8 and the sample detection chamber 2;
the straight line where the first light source component 6 and the first monochromator 7 are located is perpendicular to the straight line where the second light source component 8 and the second monochromator 9 are located, and the sample detection chamber 2 is arranged at the intersection point of the two straight lines.
The first light source component 6 and the second light source component 8 respectively illuminate the sample solution in the sample detection chamber 2 through the first monochromator 7 and the second monochromator 9, so that the sample solution in the sample detection chamber 2 is always illuminated by the light from the parallel monochromatic light, and the influence of the composite light on experimental results is reduced.
Meanwhile, through the alternate use of the first light source assembly 6 and the second light source assembly 8, the light emitted by the first light source assembly 6 and the second light source assembly 8 vertically irradiates the sample solution in the control sample detection chamber 2, so that different illumination irradiation can be performed on the sample solution under the condition that the sample solution is not moved, and the light of the first light source assembly 6 and the light of the second light source assembly 8 are not interfered with each other.
The illumination of the sample solution in the sample detection chamber 2 under different wavelengths is realized by adjusting the wavelength of the second light source component 8, and the illumination intensity and the wavelength of the first light source component 6 are adjusted, so that the first light source component 6 irradiates monochromatic light with a required specific wavelength, and the light intensity is kept stable in the irradiation time.
The temperature control component 5 is arranged at one side of the sample detection chamber 2, controls the environment of the sample solution in the sample detection chamber 2 to be at an adaptive temperature, and the adjustable temperature range of the temperature control component 5 is 0-100 ℃.
The shell 1 is a cuboid structure commonly, the whole material is ABS engineering plastics, a power supply assembly for providing constant power supply is arranged in the shell 1, the power supply assembly is electrically connected with the signal amplifying assembly 3, the processing display assembly 4, the temperature control assembly 5, the first light source assembly 6 and the second light source assembly 8 respectively, and the power supply assembly is 220v and 50Hz.
In order to ensure illumination of the first light source component 6 and the second light source component 8 to the sample solution in the sample detection chamber 2, the utility model provides a preferred scheme, the sample detection chamber 2 comprises a quartz cuvette frame 21 for placing a quartz cuvette, openings for illumination transmission are arranged around the quartz cuvette frame 21, a detector 22 is arranged on one side of the quartz cuvette frame 21 along the linear extending direction of the second light source component 8 and the second monochromator 9, and the detector 22 is electrically connected with the signal amplifying component 3;
when the detector 22 detects light from the second light source module 8, a photoelectric signal is transmitted to the signal amplifying module 3 through the detector 22.
Further, the first light source assembly 6 comprises a first spherical mirror 61 arranged on the inner side of the shell 1, a xenon lamp 62 is arranged on the mirror surface side of the first spherical mirror 61, and the center of the mirror surface side of the first spherical mirror 61 is opposite to the sample placing position of the sample detection chamber 2;
the xenon lamp 62 is connected with a rheostat 63, and one side of the quartz cuvette frame 21 is provided with a light intensity probe 23 for detecting illumination intensity along the linear extending direction of the first light source component 6 and the first monochromator 7.
The light irradiation of the xenon lamp 62 faces the first spherical mirror 61, the light irradiation intensity of the first light source assembly 6 is changed by the xenon lamp 62 through the adjusting rheostat 63, the parallel monochromatic light irradiated by the first light source assembly 6 is received by the light intensity probe 23 on one side after the sample solution in the sample detection chamber 2 is irradiated, and the light irradiation intensity of the first light source assembly 6 is adjusted to the light irradiation intensity required by detection according to the light irradiation intensity fed back by the light intensity probe 23. The first illumination component 6 is externally provided with a manual wavelength adjusting member, and can set monochromatic light with a required specific wavelength.
The first monochromator 7 comprises a first optical filter 71, a first light slit 72 and a first grating 73 which are sequentially arranged on one side of the first spherical mirror 61, and a plurality of mirrors for ensuring monochromatic light to be reflected into the sample detection chamber 2 are also arranged in the first monochromator 7.
By adjusting the mirror position in the first monochromator 7, the first light source module 6 ensures that monochromatic light passing through the first filter 71, the first slit 72 and the first grating 73 in this order can enter the sample detection chamber 2 and irradiate the sample solution by mirror reflection.
The second light source assembly 8 includes a second spherical mirror 81 disposed inside the housing 1, a deuterium lamp 82 and a tungsten lamp 83 are disposed on a mirror surface side of the second spherical mirror 81, respectively, and the deuterium lamp 82 and the tungsten lamp 83 are both connected with a control adjusting plate for adjusting wavelength.
The deuterium lamp 82 can provide light with the wavelength of 200-350nm, the tungsten lamp 83 can provide light with the wavelength of 350-800nm, and the deuterium lamp 82 and the tungsten lamp 83 are respectively regulated and controlled by the control regulating plate and can be mutually connected, so that illumination with the wavelength of 200-800nm can be provided in the second light source assembly 8 all the time.
The second monochromator 9 comprises a second optical filter 91, a second light slit 92 and a second grating 93 which are sequentially arranged on one side of the second spherical mirror 81, and a plurality of mirrors for ensuring monochromatic light to be reflected into the sample detection chamber 2 are also arranged in the second monochromator 9.
By adjusting the mirror position in the second monochromator 9, the second light source module 8 ensures that monochromatic light passing through the second optical filter 91, the second optical slit 92 and the second grating 93 in sequence can enter the sample detection chamber 2 and irradiate the sample solution by mirror reflection.
In order to ensure the temperature control of the whole experimental environment, the utility model provides a preferable scheme, wherein the side surface of the shell 1 is provided with a heat dissipation opening 11, one side of the heat dissipation opening 11 is provided with a heat dissipation fan 12, and the air port end of the heat dissipation fan 12 is opposite to the heat dissipation opening 11.
The above embodiments are only exemplary embodiments of the present utility model and are not intended to limit the present utility model, the scope of which is defined by the claims. Various modifications and equivalent arrangements of this utility model will occur to those skilled in the art, and are intended to be within the spirit and scope of the utility model.
Claims (7)
1. The protein detection device is characterized by comprising a shell (1), wherein a sample detection chamber (2) is arranged in the shell (1), the sample detection chamber (2) is connected with a processing display assembly (4) through a signal amplification assembly (3), the processing display assembly (4) is arranged on one side of the shell (1), the sample detection chamber (2) is connected with a temperature control assembly (5) for adjusting the temperature of a sample, a first light source assembly (6) and a second light source assembly (8) are respectively arranged on one side of the sample detection chamber (2), a first monochromator (7) is arranged between the first light source assembly (6) and the sample detection chamber (2), and a second monochromator (9) is arranged between the second light source assembly (8) and the sample detection chamber (2);
the straight line where the first light source component (6) and the first monochromator (7) are located is perpendicular to the straight line where the second light source component (8) and the second monochromator (9) are located, and the sample detection chamber (2) is arranged at the intersection point of the two straight lines.
2. The protein detection device according to claim 1, wherein the sample detection chamber (2) comprises a quartz cuvette frame (21) for placing a quartz cuvette, openings for light transmission are formed in the periphery of the quartz cuvette frame (21), a detector (22) is arranged on one side of the quartz cuvette frame (21) along the linear extending direction of the second light source assembly (8) and the second monochromator (9), and the detector (22) is electrically connected with the signal amplifying assembly (3);
when the detector (22) detects light from the second light source assembly (8), an optoelectronic signal is transmitted to the signal amplifying assembly (3) by the detector (22).
3. A protein detection apparatus according to claim 2, wherein the first light source assembly (6) comprises a first spherical mirror (61) arranged inside the housing (1), a xenon lamp (62) is arranged on the mirror surface side of the first spherical mirror (61), and the center of the mirror surface side of the first spherical mirror (61) is opposite to the sample placement position of the sample detection chamber (2);
the xenon lamp (62) is connected with a rheostat (63), and a light intensity probe (23) for detecting illumination intensity is arranged on one side of the quartz cuvette frame (21) along the linear extending direction where the first light source component (6) and the first monochromator (7) are located.
4. A protein detection apparatus according to claim 3, wherein the first monochromator (7) comprises a first optical filter (71), a first optical slit (72) and a first grating (73) which are sequentially arranged at one side of the first spherical mirror (61), and a plurality of mirrors for ensuring monochromatic light to reflect into the sample detection chamber (2) are further arranged in the first monochromator (7).
5. The protein detection apparatus according to claim 2, wherein the second light source assembly (8) comprises a second spherical mirror (81) disposed inside the housing (1), a deuterium lamp (82) and a tungsten lamp (83) are disposed on a mirror surface side of the second spherical mirror (81), respectively, and the deuterium lamp (82) and the tungsten lamp (83) are both connected with a control adjusting plate for adjusting a wavelength.
6. The protein detection apparatus as claimed in claim 5, wherein the second monochromator (9) comprises a second optical filter (91), a second optical slit (92) and a second grating (93) sequentially arranged at one side of the second spherical mirror (81), and a plurality of mirrors for ensuring monochromatic light to reflect into the sample detection chamber (2) are further arranged in the second monochromator (9).
7. The protein detection apparatus as claimed in claim 1, wherein the side surfaces of the housing (1) are provided with heat radiation openings (11), a heat radiation fan (12) is provided at one side of the heat radiation openings (11), and the air port ends of the heat radiation fan (12) are opposite to the heat radiation openings (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321607360.9U CN220120704U (en) | 2023-06-25 | 2023-06-25 | Protein detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321607360.9U CN220120704U (en) | 2023-06-25 | 2023-06-25 | Protein detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220120704U true CN220120704U (en) | 2023-12-01 |
Family
ID=88894299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321607360.9U Active CN220120704U (en) | 2023-06-25 | 2023-06-25 | Protein detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220120704U (en) |
-
2023
- 2023-06-25 CN CN202321607360.9U patent/CN220120704U/en active Active
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