CN218552772U - Dressing subsides for skin restoration - Google Patents
Dressing subsides for skin restoration Download PDFInfo
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- CN218552772U CN218552772U CN202222529634.9U CN202222529634U CN218552772U CN 218552772 U CN218552772 U CN 218552772U CN 202222529634 U CN202222529634 U CN 202222529634U CN 218552772 U CN218552772 U CN 218552772U
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Abstract
The utility model relates to a dressing subsides for skin restoration. The dressing patch for repairing the skin sequentially comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer, wherein the antibacterial moisture absorption dressing layer is contacted with the skin. The utility model provides a skin is restoreed and is used dressing subsides have the permeability gas good, have certain moisturizing and hygroscopicity, and antibiotic and promote wound healing's effectual, can also make the medicine slowly-releasing in addition.
Description
Technical Field
The utility model belongs to the technical field of biomedical dressing product structure, especially, relate to a dressing subsides for skin restoration.
Background
The skin is the organ of the human body with the widest distribution, is the first line of defense for protecting the human body, and is vital in maintaining homeostasis and preventing microbial infection. Skin is vulnerable to damage caused by external factors such as burns or diseases. Skin injury is usually directly exposed to the surrounding environment, and easily causes physiological disorders such as wound surface water loss, electrolyte disorder, protein loss, bacterial infection and the like. Generally, a small area of skin injury will repair itself and be easily treated, but a large area of skin injury will be difficult to heal if left untreated, will heal due to infection, and even be life threatening.
At present, the clinical treatment scheme generally uses ointments and dressings, and the traditional wound dressings such as bandages, gauzes and the like cause severe dehydration of wounds due to extremely high water absorption and high water vapor transmission rate, so that the wound environment is not favorable for cell proliferation, and bacteria can be bred.
The wound dressing is one of tissue engineering scaffolds, and can realize a wound repair function through structural design or loading of specific medicines. The main materials for preparing the dressing are generally chitosan, alginate or collagen, etc., which are made into a film type, sponge type or gel type. An excellent dressing for skin wound repair requires specific properties to achieve rapid healing of the wound. For example, the dressing needs to have good air permeability, which can help the ingrowth of new cells, granulation tissue and blood vessels at the wound site; meanwhile, the dressing also needs to have good bacteria isolation property, and an important function of the dressing is to replace skin, so that the wound surface is not directly exposed to the external environment, and the invasion and infection of pathogens in the external environment to the wound surface are avoided; the dressing also needs to have certain moisture retention, and the moist environment can ensure that the wound surface is soft and is not easy to crack and bulge; the dressing should also have certain hygroscopicity so that tissue fluid permeating from the wound surface can be quickly absorbed by the dressing to prevent the loss of water. However, the properties of the current dressing products in terms of breathability, hygroscopicity, antisepsis, or promotion of wound healing are in need of further improvement.
In summary, there is a great need to provide a new dressing patch for skin repair.
SUMMERY OF THE UTILITY MODEL
In order to solve one or more technical problem that exist among the prior art, the utility model provides a dressing subsides for skin restoration.
The utility model provides a dressing subsides for skin restoration, dressing subsides for skin restoration include antibiotic moisture absorption dressing layer, polylactic acid-glycolic acid copolymer layer and non-woven fabrics layer in proper order, antibiotic moisture absorption dressing layer contacts with skin.
Preferably, the antibacterial hygroscopic dressing layer is loaded with antibiotic liposomes; and/or the antibacterial moisture-absorbing dressing layer comprises chitosan, bacterial cellulose and polylactic acid-glycolic acid copolymer.
Preferably, the surface of the chitosan is loaded with antibiotic liposomes, and preferably, the surface of the chitosan is covalently loaded with antibiotic liposomes.
Preferably, the antibacterial moisture-absorbing dressing layer comprises a chitosan layer, a bacterial cellulose layer and a polylactic acid-glycolic acid copolymer layer in sequence, wherein the chitosan layer is more distant from the non-woven fabric layer than the bacterial cellulose layer; or the antibacterial moisture absorption dressing layer sequentially comprises a bacterial cellulose layer, a chitosan layer and a polylactic acid-glycolic acid copolymer layer, wherein the chitosan layer is closer to the non-woven fabric layer than the bacterial cellulose layer.
Preferably, the mass ratio of the chitosan layer, the bacterial cellulose layer and the polylactic acid-glycolic acid copolymer layer is (0.08-0.6): (2.5-3.5): 7.
preferably, the chitosan layer is loaded with liposomes of an antibiotic, preferably the chitosan layer is covalently loaded with liposomes of an antibiotic.
Preferably, the antibiotic contained in the antibiotic liposome is one or more of aminoglycoside, beta-lactam, glycopeptide, quinolone, sulfonamide and tetracycline antibiotics.
Preferably, the antibiotic contained in the antibiotic liposome is gentamicin, vancomycin or tetracycline hydrochloride.
Preferably, the non-woven fabric layer extends beyond the edges of the antibacterial moisture-absorbing dressing layer and the polylactic acid-glycolic acid copolymer layer; and a bonding sheet frame is arranged at the edge position of the non-woven fabric layer.
Preferably, the dressing patch for skin repair further comprises a release paper layer, wherein the release paper layer and the non-woven fabric layer are bonded together through the adhesive sheet frame, so that the antibacterial moisture-absorbing dressing layer and the polylactic acid-glycolic acid copolymer layer are wrapped inside.
Compared with the prior art, the utility model following beneficial effect has at least:
(1) The utility model provides a skin is restoreed and is included antibiotic moisture absorption dressing layer, polylactic acid-glycolic acid copolymer layer and non-woven fabrics layer in proper order with dressing subsides, antibiotic moisture absorption dressing layer contacts with skin, and wherein, non-woven fabrics layer can be used for adhering and fixing antibiotic moisture absorption dressing layer and polylactic acid-glycolic acid copolymer layer to have the advantage that the gas permeability is good, the imitative natural extracellular matrix design of polylactic acid-glycolic acid copolymer layer has hydrophobic and ventilative function, the imitative guide tissue regeneration membrane design of antibiotic moisture absorption dressing layer has certain moisturizing and hygroscopicity, and certain moist environment can guarantee that the surface of a wound is comparatively soft, is difficult for ftracture and uplift, and the dressing has certain hygroscopicity, and the tissue fluid that comes out from the surface of a wound like this can be absorbed by dressing fast, can prevent the loss of moisture, works as antibiotic liposome is carried to antibiotic moisture absorption dressing layer load, still has the antibiotic slow-release antibacterial effect of medicine, can effectively improve the antibacterial activity of dressing and improve the effect that the dressing promotes the surface of healing.
(2) The utility model provides a skin is restoreed and is pasted with dressing has fine inhibitory action to the growth and the reproduction of bacterium, to stimulating the secretion of fibroblast growth factor family (FGFs), epidermal Growth Factor (EGF), platelet Derived Growth Factor (PDGF) etc., stimulating granulation tissue to generate and organize vascularization etc. and have fine promotion effect, the utility model provides a skin is restoreed and is pasted with dressing has the permeability gas good, has certain moisture retention and hygroscopicity, and antibiotic and promotion wound healing's advantage such as effectual.
(3) In some preferred embodiments of the utility model, skin restoration is with dressing subsides still include the adhesion sheet frame and leave the type paper layer, leave the type paper layer with the non-woven fabrics layer can pass through the adhesion sheet frame bonds together, will antibiotic moisture absorption dressing layer with polylactic acid-glycolic acid copolymer layer parcel is inside, when using, tears off from type paper, can make skin restoration is with dressing subsides in skin surface of a wound department.
Drawings
The drawings of the present invention are provided for illustrative purposes only, and the proportion, size and number of the parts in the drawings are not necessarily consistent with those of actual products.
Fig. 1 is a schematic structural view of a dressing patch for skin repair according to some embodiments of the present invention.
Fig. 2 is a schematic structural view of a dressing patch for repairing skin according to another embodiment of the present invention.
Fig. 3 is a schematic plan view of a non-woven fabric layer included in a dressing patch for skin rejuvenation according to some embodiments of the present invention.
In fig. 1 to 3, 1: an antibacterial moisture-absorbing dressing layer; 11: a chitosan layer; 12: a layer of bacterial cellulose; 13: the antibacterial moisture-absorbing dressing layer comprises a polylactic acid-glycolic acid copolymer layer; 2: a polylactic acid-glycolic acid copolymer layer; 3: a non-woven fabric layer; 31: and a film sticking frame.
Detailed Description
In order to make the purpose, technical solution and advantages of the present invention clearer, the following embodiments of the present invention will be combined to clearly and completely describe the technical solution of the present invention. It is to be understood that the embodiments described are some, not all embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
The utility model provides a dressing subsides for skin restoration, for example, as shown in figure 1, dressing subsides for skin restoration include antibiotic moisture absorption dressing layer 1, polylactic acid-glycolic acid copolymer layer 2 and non-woven fabrics layer 3 in proper order, antibiotic moisture absorption dressing layer 1 contacts with skin. The utility model discloses it does not have special requirement to each layer thickness of dressing subsides for skin restoration, for example the thickness of antibiotic moisture absorption dressing layer can be 0.2 ~ 1.5mm, the thickness of polylactic acid-glycolic acid copolymer layer can be 0.1 ~ 0.2mm, the thickness on non-woven fabrics layer can be 0.1 ~ 0.5mm.
The dressing plaster for skin repair comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer in sequence, wherein the antibacterial moisture absorption dressing layer is in contact with skin, the non-woven fabric layer can be used for adhering and fixing the antibacterial moisture absorption dressing layer and the polylactic acid-glycolic acid copolymer layer and has the advantage of good air permeability, the polylactic acid-glycolic acid copolymer layer is designed by imitating a natural extracellular matrix and has the functions of hydrophobicity and air permeability, the antibacterial moisture absorption dressing layer is designed by imitating a guided tissue regeneration membrane and has certain moisture retention and moisture absorption, the wound surface can be ensured to be relatively soft and not easy to crack and bulge in certain moist environment, the dressing has certain moisture absorption, so that tissue fluid permeating from the wound surface can be rapidly absorbed by the dressing and can be prevented from losing moisture, when the antibacterial moisture absorption dressing layer is loaded with antibiotic liposome, the dressing plaster also has the effect of drug slow release and antibiosis, the antibacterial activity of the dressing can be effectively improved, and the effect of promoting wound surface healing of the dressing is improved; the utility model provides an antibiotic moisture absorption dressing layer has certain moisturizing and hygroscopicity, also can record as antibiotic moisture absorption dressing layer of moisturizing.
According to some preferred embodiments, the antibacterial hygroscopic dressing layer is loaded with antibiotic liposome, for example, the antibacterial hygroscopic dressing layer can be loaded with antibiotic liposome by soaking, coating or compounding; and/or including chitosan, bacterial cellulose and polylactic acid-glycolic acid copolymer in the antibiotic moisture absorption dressing layer in the utility model discloses in, adopt including chitosan, bacterial cellulose and polylactic acid-glycolic acid copolymer's material to make the antibiotic moisture absorption dressing layer, specifically for example can be prepared through freeze-drying process.
According to some preferred embodiments, the surface of the chitosan is loaded with antibiotic liposome, for example, the surface of the chitosan can be loaded with antibiotic liposome by soaking, coating or compounding; preferably, the chitosan is covalently loaded with antibiotic liposomes on its surface. The utility model discloses in, make antibiotic liposome load on the surface of chitosan, compare the direct mode with antibiotic load, can make the medicine slowly-releasing better, can make skin restoration paste in 24h sustained release with the dressing, reach steady state at 32h, can not have the medicine in earlier stage burst release and lead to producing the problem that the growth of biological toxicity influences the cell, can effectively improve the antibacterial activity of dressing and improve the effect that the dressing promotes the surface of a wound healing.
The utility model discloses in, make when adopting the material including chitosan, bacterial cellulose and polylactic acid-glycolic acid copolymer the antibiotic moisture absorption dressing layer, and when the surface covalence of chitosan loaded with the antibiotic liposome, can with antibiotic moisture absorption dressing layer marks as antibiotic liposome/chitosan/bacterial cellulose/polylactic acid-glycolic acid copolymer layer.
In the utility model discloses in, set up between antibiotic moisture absorption dressing layer with the non-woven fabrics layer polylactic acid-glycolic acid copolymer layer for example prepares through electrostatic spinning and forms, antibiotic liposome/chitosan/bacterial cellulose/polylactic acid-glycolic acid copolymer layer for example can prepare through freeze-drying and form, and when the antibiotic that contains in the antibiotic liposome was gentamicin, and the surperficial covalence of chitosan loaded with the antibiotic liposome, antibiotic liposome/chitosan/bacterial cellulose/polylactic acid-glycolic acid copolymer layer with hug closely the preparation process of polylactic acid-glycolic acid copolymer layer that the non-woven fabrics layer set up can be:
(1) preparation of thiolated chitosan: uniformly mixing chitosan (CS: the molecular weight is 416kDa, the deacetylation degree is 88%), 2-iminothiolane hydrochloride, 4-dimethylaminopyridine (catalyst) and Dithiothreitol (DTT) by using a phosphate buffered saline solution with the concentration of 0.1mM and the pH of 7 to obtain a mixed solution, and then reacting the mixed solution at 37 ℃ for 3 hours to obtain thiolated chitosan; the concentration of 2-imino-thiacyclopentane hydrochloride contained in the mixed solution is 20mM, the concentration of 4-dimethylamino pyridine contained in the mixed solution is 20mM, and the concentration of dithiothreitol contained in the mixed solution is 20mM; when the chitosan is subjected to sulfhydrylation, the chitosan is used in an amount such that the molar ratio of amino groups contained on the chitosan to dithiothreitol is 1:1.
(2) Preparation of gentamicin liposome: liposomes 1,2-dipalmitoyl-sn-glycerol-3-phosphorylcholine, cholesterol, 1,2-distearoyl-sn-glycerol-3-phosphoethanolamine-N- [ maleimide (polyethylene glycol) -2000] (ammonium salt), L- α -phosphatidylethanolamine and 3,3' -di-N-octadecyloxycarbonylcyanine perchlorate (green fluorescence) were dissolved in a 1: at 60%, the solution was pushed back and forth 20 times through a porous polycarbonate membrane (100 nm) fixed between two syringes at 60 ℃ and extruded through the porous polycarbonate membrane to form unilamellar liposomes (gentamicin liposomes).
(3) Preparation of chitosan covalently loaded with antibiotic liposomes: adding the gentamicin liposome obtained in the step (2) into the thiolated chitosan obtained in the step (1) to react to obtain chitosan covalently loaded with antibiotic liposome; the dosage of the thiolated chitosan and the gentamicin liposome is such that the molar ratio of sulfydryl contained in the thiolated chitosan to maleimide groups contained in the antibiotic liposome is 1:1; the reaction of the thiolated chitosan and the gentamicin liposome takes phosphate buffered salt solution with the concentration of 0.1mM and the pH of 7 as a solvent, the reaction is carried out for 2h at the room temperature of 25 ℃, then the reaction product is washed for 5 times by phosphate buffered salt solution (pH = 7) with 1min each time, the washing process is gently stirred by a glass rod, and finally the chitosan covalently loaded with the antibiotic liposome is obtained through drying.
(4) Electrostatic spinning preparation film material (outer layer of dressing paste for skin repair)
(a) Dissolving 2g of PLGA in 10mL of hexafluoroisopropanol, and fully and uniformly stirring to obtain a PLGA solution (electrospinning solution) with the concentration of 20w/v%, wherein the Mw of the PLGA is =20 ten thousand, and the LA/GA = 50;
(b) The humidity of the spinning chamber is controlled to be maintained at 45% by adjusting a humidifier and a dehumidifier of the spinning chamber, and the temperature in the spinning chamber is maintained at about 25 ℃ by adjusting an air conditioner; placing the micro syringe pump at one side of the receiver (cylindrical receiver) and connecting the receiver and the high-voltage power supply with the ground wire;
(c) Filling the prepared electrospinning liquid into a 10mL syringe, connecting a 21G stainless steel needle, and connecting a chuck connected with a high-voltage direct-current power supply with the needle of the syringe; setting the flow rate to be 1mL/h, fixing the micro injection pump on the micro injection pump, setting the inner diameter of the injector to be 14.90mm, the voltage to be 20kV, the receiving distance to be 20cm, the rotating speed of the receiver to be 150r/min, coating the receiver with aluminum foil, setting the diameter of the receiver to be 8cm, and scratching the receiver along the axis direction after the electrospinning is finished to obtain the film-shaped material.
(5) The preparation of the chitosan solution covalently loaded with the antibiotic liposome comprises the following steps: weighing chitosan covalently loaded with antibiotic liposome, adding the chitosan into acetic acid aqueous solution with the mass fraction of 1%, and placing the chitosan on a magnetic stirrer to stir for 60min to obtain the chitosan solution covalently loaded with antibiotic liposome with the mass concentration of 1%; the preparation of the cellulose solution is as follows: adding bacterial cellulose BC into NaOH aqueous solution with the mass fraction of 1%, soaking for 24h, placing on a filter screen, washing with ultrapure water, repeatedly washing until the pH value is neutral, and drying for later use; weighing BC soaked in a sodium hydroxide solution, adding ultrapure water, and carrying out uniform dispersion for 10min at a speed of 20000r/min by using a high-speed dispersion homogenizer to obtain a cellulose solution with the mass fraction of 1%; the preparation of the polylactic acid-glycolic acid copolymer solution comprises the following steps: PLGA was dissolved in hexafluoroisopropanol and stirred well to obtain a 20w/v% concentration polylactic acid-glycolic acid copolymer solution using PLGA having Mw =20 ten thousand and LA/GA = 50.
(6) Mixing the polylactic acid-glycolic acid copolymer solution, the cellulose solution and the chitosan solution which is covalently loaded with the antibiotic liposome and prepared in the step (5) according to the mass ratio of the contained polylactic acid-glycolic acid copolymer, the bacterial cellulose and the chitosan which is covalently loaded with the antibiotic liposome of 7; the freeze drying comprises a pre-freezing stage, a first sublimation stage, a second sublimation stage and a temperature reduction stage, and the process conditions of each stage are as follows:
a pre-freezing stage: the target temperature is-10 ℃, the speed is 3.5 ℃/min, and the constant temperature duration is 300min;
a first sublimation stage: vacuumizing, aerating at 100Pa, controlling the target temperature to be-3 ℃, the speed to be 0.7 ℃/min and the constant temperature duration to be 1300min;
the second sublimation stage, evacuation, aerify 100Pa, including five intensification ladders, do respectively:
the speed is 0.2 ℃/min at 0 ℃, and the constant temperature duration is 120min;
the speed is 1.0 ℃/min at 10 ℃, and the constant temperature duration is 120min;
the speed is 1.0 ℃/min at 20 ℃, and the constant temperature duration is 120min;
the speed is 1.0 ℃/min at 30 ℃, and the constant temperature duration is 120min;
40 ℃, the speed is 1.0 ℃/min, the constant temperature duration is as follows: judging the end point every 10 minutes until the end point is qualified; the end point is judged to be less than or equal to 0.9Pa/10min;
and (3) cooling: cooling to room temperature at a rate of 1.5 deg.C/min.
According to some preferred embodiments, the antimicrobial moisture-absorbing dressing layer comprises, in order, a chitosan layer, a bacterial cellulose layer and a polylactic acid-glycolic acid copolymer layer, the chitosan layer being further from the nonwoven layer than the bacterial cellulose layer, for example, as shown in fig. 2. The utility model discloses in, antibiotic moisture absorption dressing layer is including the bacterial cellulose layer, the bacterial cellulose layer can participate in extracellular matrix synthesis, has fine promotion effect to the secretion of stimulation fibroblast growth factor family (FGFs), epidermal Growth Factor (EGF), platelet Derived Growth Factor (PDGF) etc. stimulates granulation tissue to produce and organize vascularization etc.; the utility model discloses in, when antibiotic moisture absorption dressing layer includes chitosan layer, bacterial cellulose layer and polylactic acid-glycolic acid copolymer layer in proper order, each layer can be respectively through freeze-drying filming back, and rethread conventional mode is in the same place compound, does not have special requirement to specific freeze-drying condition, adopt conventional operation can.
According to some preferred embodiments, the antimicrobial moisture-absorbing dressing layer comprises, in order, a bacterial cellulose layer, a chitosan layer, and a polylactic acid-glycolic acid copolymer layer, the chitosan layer being closer to the nonwoven layer than the bacterial cellulose layer.
According to some preferred embodiments, the mass ratio of the chitosan layer, the bacterial cellulose layer and the polylactic acid-glycolic acid copolymer layer is (0.08-0.6): (2.5-3.5): 7, for example, can be 0.01.
According to some preferred embodiments, the chitosan layer is loaded with antibiotic liposomes, preferably the chitosan layer is covalently loaded with antibiotic liposomes. In the present invention, when the chitosan layer is covalently loaded with the antibiotic liposome, the chitosan layer is formed using chitosan covalently loaded with the antibiotic liposome; the utility model discloses in, chitosan layer covalent load has the antibiotic liposome, compares through physical adsorption for example surface coating's mode with antibiotic load in the mode on chitosan layer surface, can make the medicine slowly-releasing better, can make skin restoration paste the sustained release in 24h with dressing, reaches stable state at 32h, can not have the medicine burst release in earlier stage and lead to producing the problem of the growth of biotoxicity influence cell, can effectively improve the antibacterial activity of dressing and improve the effect that the dressing promotes the surface of a wound healing.
According to some preferred embodiments, the antibiotic contained in the antibiotic liposome is one or more of aminoglycoside, β -lactam, glycopeptide, quinolone, sulfonamide, and tetracycline antibiotics.
According to some embodiments, the antibiotic contained in the antibiotic liposome is gentamicin, vancomycin, or tetracycline hydrochloride.
According to some preferred embodiments, for example, as shown in fig. 3, the nonwoven layer extends beyond the edges of the antimicrobial moisture-absorbing dressing layer and the polylactide-co-glycolide layer; and a bonding sheet frame is arranged at the edge position of the non-woven fabric layer.
According to some preferred embodiments, the dressing patch for skin repair further comprises a release paper layer, wherein the release paper layer and the non-woven fabric layer are bonded together through the adhesive sheet frame, so that the antibacterial moisture-absorbing dressing layer and the polylactic acid-glycolic acid copolymer layer are wrapped inside.
The invention will be further described below by way of example, but the scope of protection of the invention is not limited to these embodiments.
Example 1
A dressing patch for skin repair sequentially comprises an antibiotic liposome/chitosan/bacterial cellulose/polylactic acid-glycolic acid copolymer layer (namely an antibacterial moisture absorption dressing layer), a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer, wherein the antibiotic liposome/chitosan/bacterial cellulose/polylactic acid-glycolic acid copolymer layer is in contact with the skin, and the surface of chitosan is covalently loaded with the antibiotic liposome (gentamicin liposome).
Example 2
A dressing patch for skin repair comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer in sequence, wherein the antibacterial moisture absorption dressing layer is contacted with skin; the antibacterial moisture-absorption dressing layer sequentially comprises a chitosan layer, a bacterial cellulose layer and a polylactic acid-glycolic acid copolymer layer, the chitosan layer is farther away from the non-woven fabric layer than the bacterial cellulose layer, and antibiotic liposome (gentamicin liposome) is covalently loaded on the chitosan layer; the mass ratio of the chitosan layer, the bacterial cellulose layer and the polylactic acid-glycolic acid copolymer layer is 0.2.
Example 3
A dressing patch for skin repair comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer in sequence, wherein the antibacterial moisture absorption dressing layer is contacted with skin; the antibacterial moisture-absorption dressing layer sequentially comprises a bacterial cellulose layer, a chitosan layer and a polylactic acid-glycolic acid copolymer layer, the chitosan layer is closer to the non-woven fabric layer than the bacterial cellulose layer, and antibiotic liposome (gentamicin liposome) is covalently loaded on the chitosan layer; the mass ratio of the chitosan layer, the bacterial cellulose layer and the polylactic acid-glycolic acid copolymer layer is 0.2.
Example 4
A dressing patch for skin repair comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer in sequence; the antibacterial moisture-absorption dressing layer sequentially comprises a chitosan layer and a polylactic acid-glycolic acid copolymer layer, the chitosan layer included in the antibacterial moisture-absorption dressing layer is contacted with the skin, and the chitosan layer is covalently loaded with antibiotic liposome (gentamicin liposome); in the antibacterial moisture-absorbing dressing layer, the mass ratio of the chitosan layer to the polylactic acid-glycolic acid copolymer layer is 0.2.
Example 5
A dressing patch for skin repair comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer in sequence, wherein the antibacterial moisture absorption dressing layer is contacted with skin; the antibacterial moisture-absorbing dressing layer sequentially comprises a chitosan layer, a bacterial cellulose layer and a polylactic acid-glycolic acid copolymer layer, the chitosan layer is farther away from the non-woven fabric layer than the bacterial cellulose layer, the surface of the chitosan layer is coated with an antibiotic liposome (gentamicin liposome), and the dosage of the gentamicin liposome is 10% of the mass of the chitosan layer; the mass ratio of the chitosan layer, the bacterial cellulose layer and the polylactic acid-glycolic acid copolymer layer is 0.2.
Example 6
A dressing patch for skin repair comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer in sequence, wherein the antibacterial moisture absorption dressing layer is contacted with skin; the antibacterial moisture-absorption dressing layer sequentially comprises a chitosan layer, a bacterial cellulose layer and a polylactic acid-glycolic acid copolymer layer, the chitosan layer is farther away from the non-woven fabric layer than the bacterial cellulose layer, the surface of the chitosan layer is coated with antibiotic (gentamicin), and the using amount of the gentamicin is 10% of the mass of the chitosan layer; the mass ratio of the chitosan layer, the bacterial cellulose layer and the polylactic acid-glycolic acid copolymer layer is 0.2.
Example 7
Referring to CN114073786A, the imbibing antibacterial medical dressing obtained in example 1 sequentially comprises a moisture absorption antibacterial layer, a polylactic acid/polycaprolactone nanofiber membrane and a non-woven fabric layer, wherein the moisture absorption antibacterial layer comprises an antibacterial drug and polyvinyl alcohol/chitosan nano short fibers, and is in contact with skin.
Detecting the antibacterial activity of the dressing patch for skin repair (bacteriostasis method): the antibacterial activity test was carried out in Nutrient Agar (NA) medium, and the sample was cut into a disk shape having a diameter of 6 mm. After sterilization, the discs were each placed in a container containing about 10 6 Incubating CFU/mL agar plates of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa at 37 +/-1 ℃ for 24 hours, and measuring the edge distance of a bacteriostatic circle formed around a wafer, wherein the unit is mm; blank set is an unpopulated disc, which directly will contain about 10 6 CFU/mL of agar plates of Escherichia coli, staphylococcus aureus and Pseudomonas aeruginosa were incubated at 37. + -. 1 ℃ for 24 hours, and the results are shown in Table 1; the utility model discloses in, this edge is apart from the radius difference that the radius of the radius that the size indicates the antibacterial zone that forms and the radius of disk.
Table 1: the results of the edge spacing of the antimicrobial circles of the dressing patches of examples 1-4.
| Examples | Escherichia coli | Staphylococcus aureus | Pseudomonas aeruginosa |
| Example 1 | 3.755mm | 3.691mm | 4.883mm |
| Example 2 | 3.743mm | 3.675mm | 4.876mm |
| Example 3 | 3.668mm | 3.634mm | 4.811mm |
| Example 4 | 3.758mm | 3.703mm | 4.889mm |
| Blank group | 0 | 0 | 0 |
Detecting the healing performance of the dressing paste:
1. test materials: the dressing patches for skin repair in examples 1-7.
2. The test method comprises the following steps: fibroblast cells of neonatal skin as cultured cells in DMEM high-glucose culture medium containing 10% by mass of neonatal bovine serum, the volume fraction of which is 5% by volume of CO 2 Culturing for 24 hours at 37 ℃, then respectively inoculating the materials to a bottom layer, paving a layer of dressing plaster corresponding to the materials in the embodiments 1-7, and culturing for 48 hours in DMEM high-sugar culture solution containing 0.5 mass percent of newborn bovine serum; the control blank (blank) was inoculated into DMEM high-glucose culture medium containing 0.5% newborn calf serum without any dressing patch and cultured for 48h, and the proliferation rate in Table 2 is the proliferation rate relative to the blank. The cells were counted by a conventional cell counting method, and the proliferation effect was shown in Table 2.
Moisture absorption Performance and moisture Retention Performance test
1. Test materials: the dressing of examples 1-7.
2. The test method comprises the following steps: the dressing patches of examples 1-7 were tested for their water absorption rate and retention capacity as follows:
(1) water absorption rate: the test material was cut into 4cm × 4cm test pieces by placing about 10mL of physiological saline (0.9% sodium chloride) in a stainless steel container, sealed in the container, and stored for 8 hours, the weight of the test piece before being placed in the container was m l (g) And the weight of the test piece taken out after 8 hours is m 2 (g) Water absorption capacity Q = (m) 2 -m 1 )/m l ；
(2) Liquid retention capacity: the test material was cut into test pieces of 7cm × 10cm × 0.1cm by placing L1 (mL) of physiological saline (0.9% sodium chloride) in a stainless steel container, sealing the test pieces in the container and storing the test pieces for 8 hours, and after removing the test pieces, the amount of remaining physiological saline L2 (mL) was measured and the liquid retention capacity = (L2-L1)/(7 × 10 × 0.1) × 10 3 (L/m 3 ) The results are shown in Table 2; in Table 2, the symbol "-" indicates that the performance index was not tested.
Table 2: performance of the patches of examples 1-7.
Testing the slow release performance of the medicament: the dressing patch with an area of 1cm × 1cm was immersed in a test tube containing 4mL of PBS buffer (pH = 7.4), the test tube was placed in a constant temperature shaker at 37 ℃, the shaker was oscillated at a frequency of 60 times/min, 400 μ L of liquid was extracted at a specific time point, the gentamicin content was analyzed, and 400 μ L of fresh PBS buffer was supplemented after each liquid extraction to maintain the total volume of the release medium at 4mL. 400. Mu.L of the extract, 350. Mu.L of isopropyl alcohol and 250. Mu.L of o-phthalaldehyde standard solution were put into a centrifuge tube, incubated at room temperature for 30min, and then absorbance was measured at about 332nm by high performance liquid chromatography-ultraviolet detector, and the results of the dressing patches of example 1 and example 2 are shown in Table 3.
As can be seen from the results in Table 3, the dressing for skin repair of the present invention has the function of sustained release of the drug, and the dressing for skin repair of some embodiments of the present invention is continuously released in 24h to reach the stable state in 32 h.
The detailed description of the present invention is the technology known to those skilled in the art.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although described in detail with reference to the foregoing embodiments, those of ordinary skill in the art will understand that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (3)
1. The utility model provides a dressing subsides for skin restoration which characterized in that:
the dressing patch for repairing the skin sequentially comprises an antibacterial moisture absorption dressing layer, a polylactic acid-glycolic acid copolymer layer and a non-woven fabric layer, wherein the antibacterial moisture absorption dressing layer is contacted with the skin;
the antibacterial moisture-absorption dressing layer sequentially comprises a chitosan layer, a bacterial cellulose layer and a polylactic acid-glycolic acid copolymer layer, and the chitosan layer is farther away from the non-woven fabric layer than the bacterial cellulose layer; the surface of the chitosan layer is coated with antibiotic liposome.
2. The dressing patch for skin rejuvenation according to claim 1, wherein:
the non-woven fabric layer exceeds the edges of the antibacterial moisture-absorbing dressing layer and the polylactic acid-glycolic acid copolymer layer;
and a bonding sheet frame is arranged at the edge position of the non-woven fabric layer.
3. The dressing patch for skin repair according to claim 2, characterized in that:
the dressing patch for repairing skin further comprises a release paper layer, wherein the release paper layer and the non-woven fabric layer are bonded together through the adhesive sheet frame, so that the antibacterial moisture-absorbing dressing layer and the polylactic acid-glycolic acid copolymer layer are wrapped inside.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202222529634.9U CN218552772U (en) | 2022-09-23 | 2022-09-23 | Dressing subsides for skin restoration |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222529634.9U CN218552772U (en) | 2022-09-23 | 2022-09-23 | Dressing subsides for skin restoration |
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| CN218552772U true CN218552772U (en) | 2023-03-03 |
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| CN202222529634.9U Active CN218552772U (en) | 2022-09-23 | 2022-09-23 | Dressing subsides for skin restoration |
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