CN218350105U - Platelet function detection device - Google Patents
Platelet function detection device Download PDFInfo
- Publication number
- CN218350105U CN218350105U CN202220378344.6U CN202220378344U CN218350105U CN 218350105 U CN218350105 U CN 218350105U CN 202220378344 U CN202220378344 U CN 202220378344U CN 218350105 U CN218350105 U CN 218350105U
- Authority
- CN
- China
- Prior art keywords
- blood sample
- module
- channel
- micro
- platelet function
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a platelet function detection device, which comprises a blood sample providing module, a flow detection module, an image acquisition module and a data processing module; the blood sample providing module is used for providing a blood sample and comprises a power pump; the flow detection module comprises a micro-fluidic chip and a waste liquid pool, wherein the micro-fluidic chip is provided with an inlet part and an outlet part, the micro-fluidic chip is provided with a channel for the flow of the blood sample, the inlet part is communicated with a power pump, the power pump pumps the blood sample into the micro-fluidic chip through the inlet part, and the outlet part is communicated with the waste liquid pool; the image acquisition module is used for acquiring a fluorescence image of a blood sample flowing through the channel and comprises a fluorescence microscope and a camera; the data processing module is used for carrying out quantitative analysis processing on the fluorescence image acquired by the image acquisition module. The utility model has the advantages of short detection time, no need of complex specimen treatment and bedside detection.
Description
Technical Field
The utility model belongs to the technical field of the platelet function detects, concretely relates to platelet function detection device.
Background
Platelet activation plays a key role in various arterial thrombotic diseases, and antiplatelet treatment becomes an important means for secondary prevention and treatment of thrombotic diseases such as coronary heart disease, cerebral infarction and the like.
However, there is some variability in the response of different individuals to antiplatelet drug therapy, i.e., diversity in platelet response. Research finds that the hyperreactivity of the residual platelet is related to the increased risk of cardiovascular adverse events after PCI operation, such as thrombus in a stent, myocardial infarction, cerebral apoplexy and the like. Low reactivity of residual platelets increases the risk of bleeding.
Platelet Function Tests (PFT) are of great value in assessing Platelet reactivity in patients receiving antiplatelet therapy and predicting the occurrence of thrombotic and hemorrhagic events. However, currently no positive results are obtained from large prospective clinical studies that guide individualized antiplatelet therapy based on PFT results. The reason for this analysis is mainly due to the inherent drawbacks of the existing detection methods themselves. The key to achieving accurate individualized anti-platelet therapy lies in the development of more sensitive and reliable PFT techniques. Many receptors are present on the platelet surface, which cause platelet activation when the chemical composition of the blood or the shear rate of the fluid changes, resulting in arterial thrombosis. Most of the existing PFT technologies affect a single signal path through platelet agonists under a static condition to realize platelet function detection, and the whole platelet function is difficult to be comprehensively reflected.
The micro-fluidic chip technology is a micron-scale processing technology with multifunctional integration and automatic analysis, and has the unique advantages of low consumption, fast detection, controllable flow and the like, so that the micro-fluidic chip technology is widely concerned and applied in the field of biomedicine.
In the prior art, a micro-fluidic chip base is adopted to simulate an in-vivo narrow arteriole network, so that the measurement of blood coagulation and platelet functions in a human body is realized by simulating human body pathological and physiological flow environments under an in-vitro condition; and a collagen coating or (and) a fibrin coating microfluidic channel is also used for detecting the platelet aggregation function.
For example, chinese patent 202120541348.7 discloses a microfluidic chip and a platelet function detection device, which comprises a platelet separation and enrichment module, a microfluidic shear force control module and a collagen control module, wherein red, white blood cells and platelets are separated by using a microfluidic inertial force induction mode, and the platelets are activated by a large shear force in a microchannel by designing a microchannel aspect ratio; the collagen control module enables the platelets to generate an adhesion aggregation phenomenon, the fluorescence microscopic component can detect the aggregation area and height of the platelets after fluorescent staining, the aggregation rate of the platelets is calculated, and the mapping relation between the time of thrombus formation and the collected image is established to reflect the adhesion aggregation function level of the platelets.
In the prior art, the platelet aggregation function is still analyzed after a platelet inducer coating is adopted in a plurality of researches for detecting the platelet function by adopting a microfluidic chip technology. Although detection in a flow environment is achieved compared to traditional PFT, the limitations of affecting platelet function through a single signal-mediated pathway have not been overcome.
The application improves prior art, provides one kind and need not chemical inducer, can carry out the platelet function detection device of short-term test.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a detection time is short, need not complicated sample and handle and can realize the platelet function detection device that the bedside detected.
In order to achieve the above object, the present invention provides a platelet function detecting device, which comprises a blood sample providing module, a flow detecting module, an image collecting module and a data processing module;
the blood sample providing module is used for providing a blood sample and comprises a power pump;
the flow detection module comprises a micro-fluidic chip and a waste liquid pool, the micro-fluidic chip is provided with an inlet part and an outlet part, the micro-fluidic chip is provided with a channel for the flow of the blood sample, the inlet part is communicated with a power pump, the power pump pumps the blood sample into the micro-fluidic chip through the inlet part, and the outlet part is communicated with the waste liquid pool;
the image acquisition module is used for acquiring a fluorescence image of a blood sample flowing through the channel and comprises a fluorescence microscope and a camera;
the data processing module is used for carrying out quantitative analysis processing on the fluorescence image acquired by the image acquisition module.
In the technical scheme of the utility model, the integrated design of the detection device has the advantages of rapidness, simplicity, convenience, short detection time, no need of complex sample treatment and realization of bedside detection; the microfluidic technology is utilized, expensive reagents, antibodies and the like are not needed in the detection process, and the method has the advantages of low detection cost and considerable market prospect.
As another specific embodiment of the utility model, the power pump is a micro-injection pump with adjustable power.
In the scheme, the blood sample can enter the microfluidic chip at different fluid shear rates by changing the power of the micro-injection pump, namely, the adhesion and aggregation behaviors of the blood platelets can be analyzed at different shear rates by setting the initial shear rate of the flowing blood sample, and the simulation of the fluid environment in a pathological state is realized in vitro.
As another embodiment of the present invention, the image acquisition module further comprises a light source for illumination, and the light source acts on the fluorescence microscope.
As another specific embodiment of the present invention, the data processing module at least includes an image processor, and the image processor is connected to the camera through data.
As another specific embodiment of the present invention, the channel includes a main channel and a plurality of parallel microchannels, and the plurality of microchannels are all communicated with the main channel, wherein the fluorescence image of the blood sample passing through the plurality of microchannels is collected by the fluorescence microscope and the camera.
As another embodiment of the present invention, the channel further comprises an outlet channel, and the plurality of microchannels are communicated with the outlet channel, wherein the outlet channel is communicated with the waste liquid tank through the outlet portion.
As another embodiment of the present invention, the inner surface of the microchannel is provided with or formed with a super-hydrophobic layer.
The utility model discloses possess following beneficial effect:
1) Is quick and simple: the detection time is short, complex specimen processing is not needed, and bedside detection can be realized;
2) Good economy: the micro-fluidic technology is a mature technology widely applied in the medical field, does not need a chemical inducer, does not need expensive reagents, antibodies and the like in the detection process, and has low detection cost and considerable market prospect;
the present invention will be described in further detail with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of a framework of a platelet function testing device according to the present invention;
FIG. 2 is a schematic connection diagram of the platelet function testing device of the present invention;
fig. 3 is a schematic diagram of a microfluidic chip used in the platelet function testing device of the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.
Example 1
The present embodiment provides a platelet function testing device, as shown in fig. 1-3, which includes a blood sample providing module 100, a flow testing module 200, an image collecting module 300 and a data processing module 400;
the blood sample providing module 100 is for providing a blood sample, and includes a power pump 110;
the flow detection module 200 comprises a microfluidic chip 210 and a waste liquid pool 220, as shown in fig. 2, the microfluidic chip 210 has an inlet portion 211 and an outlet portion 212, while the microfluidic chip 210 has a channel 213 for flowing the blood sample, the inlet portion 211 is connected to the power pump 110, the power pump 110 pumps the blood sample into the microfluidic chip 210 through the inlet portion 211, and the outlet portion 212 is connected to the waste liquid pool 220;
preferably, the power pump 110 is a power-adjustable micro-syringe pump, and the power of the micro-syringe pump is changed to enable the blood sample to enter the microfluidic chip 210 at different fluid shear rates, that is, the adhesion and aggregation behaviors of platelets are analyzed at different shear rates by setting the initial shear rate of the flow of the blood sample, and the simulation of the fluid environment in a pathological state is realized in vitro.
Further, as shown in fig. 3, the channel 213 includes a main channel 2131 and a plurality of parallel micro channels 2132, for example, four micro channels 2132, and the plurality of micro channels 2132 are all in communication with the main channel 2131, wherein a fluorescence image of the blood sample passing through the plurality of micro channels 2132 is collected by a fluorescence microscope and a camera.
Still further, channel 213 further comprises an outlet channel 2133, and the plurality of microchannels 2132 are all in communication with outlet channel 2133, wherein outlet channel 2133 is in communication with waste reservoir 220 via outlet portion 212.
Specifically, the inner surfaces of microchannels 2132 are provided or formed with a superhydrophobic layer.
The super-hydrophobic layer is made of a material obtained by changing the wettability and the roughness of a PDMS (polydimethylsiloxane, which belongs to hydrophobic organic silicon materials) surface material by means of an ultraviolet laser photoetching technology, and can activate platelets in a short time without a chemical inducer.
Correspondingly, the microchannel 2132 with the super-hydrophobic layer can better simulate the intravascular environment in physiological and pathological states in vitro, and can detect the adhesion and aggregation functions of platelets without using a chemical activator, thereby being beneficial to detection.
The image acquisition module 300 is used for acquiring a fluorescence image of a blood sample flowing through a channel, and comprises a fluorescence microscope 310, a camera 320 and a light source 330, wherein the light source acts on the fluorescence microscope.
Wherein, the platelets after fluorescent labeling and dyeing show strong green fluorescence under the fluorescent microscope 310, and fluorescent images of the surfaces of the microchannels at various time points under the flowing state are captured through the fluorescent microscope 310 and the (high-speed) camera 320, so that the adhesion and aggregation behaviors of the platelets on the surfaces of the channels under the flowing state are observed.
The data processing module 400 is configured to perform quantitative analysis processing on the fluorescence image acquired by the image acquisition module 300, wherein the data processing module 400 at least includes an image processor 410, the image processor 410 is connected to the camera 320 for performing quantitative analysis on the collected fluorescence image by using the image processor 410, so as to perform efficient, fast and accurate quantization on the fluorescence image, calculate the platelet coverage on the channel surface, and perform quantitative analysis.
In the embodiment, the micro-fluidic technology is adopted, the whole platelet aggregation function is detected in an in-vitro flow state, and the influence of the change of the shear rate on the platelet aggregation function is analyzed by simulating different human body flow environments.
Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the scope of the invention. Any person skilled in the art can make some modifications without departing from the scope of the invention, i.e. all equivalent modifications made according to the invention are intended to be covered by the scope of the invention.
Claims (7)
1. A platelet function detection device is characterized by comprising a blood sample providing module, a flow detection module, an image acquisition module and a data processing module;
the blood sample providing module is used for providing a blood sample and comprises a power pump;
the flow detection module comprises a micro-fluidic chip and a waste liquid pool, the micro-fluidic chip is provided with an inlet part and an outlet part, the micro-fluidic chip is provided with a channel for the flow of a blood sample, the inlet part is communicated with the power pump, the power pump pumps the blood sample into the micro-fluidic chip through the inlet part, and the outlet part is communicated with the waste liquid pool;
the image acquisition module is used for acquiring a fluorescence image of the blood sample flowing in the channel and comprises a fluorescence microscope and a camera;
the data processing module is used for carrying out quantitative analysis processing on the fluorescence image acquired by the image acquisition module.
2. A platelet function testing device according to claim 1 wherein said powered pump is a power adjustable micro syringe pump.
3. The platelet function testing device of claim 1, wherein the image acquisition module further comprises a light source for illuminating, the light source acting on the fluorescence microscope.
4. A platelet function testing device as claimed in claim 1 wherein said data processing module includes at least an image processor in data communication with said camera.
5. The platelet function testing device of claim 1, wherein said channel comprises a main channel and a plurality of parallel microchannels, each of said plurality of microchannels being in communication with said main channel, wherein a fluorescence image of a blood sample passing through said plurality of microchannels is acquired by said fluorescence microscope and camera.
6. A platelet function testing device according to claim 5 wherein said channel further comprises an outlet channel, each of said plurality of microchannels being in communication with said outlet channel, wherein said outlet channel is in communication with said waste reservoir via said outlet portion.
7. A platelet function testing device according to claim 5 wherein an inner surface of said microchannel is provided with or formed with a superhydrophobic layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220378344.6U CN218350105U (en) | 2022-02-23 | 2022-02-23 | Platelet function detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220378344.6U CN218350105U (en) | 2022-02-23 | 2022-02-23 | Platelet function detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218350105U true CN218350105U (en) | 2023-01-20 |
Family
ID=84922132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220378344.6U Active CN218350105U (en) | 2022-02-23 | 2022-02-23 | Platelet function detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218350105U (en) |
-
2022
- 2022-02-23 CN CN202220378344.6U patent/CN218350105U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9378557B2 (en) | Microfluidic device for assessing object/test material interactions | |
| US6251615B1 (en) | Cell analysis methods | |
| JP2736091B2 (en) | Elements and methods for accurately, quickly and simply performing a bioassay | |
| JP2021009153A (en) | Low volume coagulation assay | |
| JP2634219B2 (en) | Accurate, fast and simple method for performing coagulation assays using dry chemical reagents and paramagnetic particles | |
| US20020028471A1 (en) | Cell analysis methods and apparatus | |
| US20040131500A1 (en) | Device and method for evaluating platelets | |
| EP2021491A2 (en) | Detecting tumor biomarker in oral cancer | |
| US20120244630A1 (en) | Multiplexed analyte concentration measurement | |
| CN112986063B (en) | High-throughput chromosome and cytoskeleton strain flow analyzer and implementation method | |
| EP3060648A1 (en) | Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells | |
| GB2423266A (en) | A structure and method for detecting an analyte in a microfluidic chip | |
| WO2007037530A1 (en) | Method of measuring biomolecular reaction at ultrahigh speed | |
| CN108445200A (en) | A kind of influence based on micro-fluidic chip detection pentoxifylline to Erythrocytes from Coronary Heart Disease deformability and biochemical index | |
| An et al. | Measuring cell deformation by microfluidics | |
| Kang | Microfluidic-based effective monitoring of bloods by measuring RBC aggregation and blood viscosity under stepwise varying shear rates | |
| CN218350105U (en) | Platelet function detection device | |
| EP1057004A1 (en) | Cell analysis methods and apparatus | |
| Linert | Applications of Microfluidics and Nanotechnologies for Point-of-Care Devices | |
| EP2845002B1 (en) | Method to analyze the cluster formation process in a biological fluid and corresponding analysis apparatus | |
| CN112924431A (en) | Microfluid chip and platelet function detection device | |
| CN110208534A (en) | Self-priming Diagnostic Value of Several Serum Tumor Markers Multi-example detection chip | |
| KR20150120564A (en) | In-line lab-on-a-chip system for red blood cell membrane slip rate mesurement, and there of optical detection system | |
| CN104611190A (en) | Detection chip determining cell alpha-naphthyl batyrate esterase and reagent thereof | |
| EP4179335A1 (en) | Method for evaluating the metabolic activity of a non-cancer cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |