CN2091865U - Device for shooting corpuscle by impact - Google Patents
Device for shooting corpuscle by impact Download PDFInfo
- Publication number
- CN2091865U CN2091865U CN91207467U CN91207467U CN2091865U CN 2091865 U CN2091865 U CN 2091865U CN 91207467 U CN91207467 U CN 91207467U CN 91207467 U CN91207467 U CN 91207467U CN 2091865 U CN2091865 U CN 2091865U
- Authority
- CN
- China
- Prior art keywords
- gun barrel
- stopper
- seat
- particulate
- ring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004677 Nylon Substances 0.000 claims abstract description 16
- 229920001778 nylon Polymers 0.000 claims abstract description 16
- 239000007787 solid Substances 0.000 claims abstract description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 238000013022 venting Methods 0.000 claims description 7
- 239000012472 biological sample Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 4
- 239000003721 gunpowder Substances 0.000 abstract description 8
- 239000000779 smoke Substances 0.000 abstract description 4
- 230000000903 blocking effect Effects 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 230000035939 shock Effects 0.000 abstract 1
- 108700026220 vif Genes Proteins 0.000 abstract 1
- 239000002245 particle Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 5
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 3
- 229910052721 tungsten Inorganic materials 0.000 description 3
- 239000010937 tungsten Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 2
- 241000234282 Allium Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a device for shooting corpuscles by impact, which belongs to the technical field of a corpuscle shooting device in genetic engineering. The utility model adopts measures that solid blocking blocks are adopted to separate nylon balls from corpuscle solids carrying genes, a basic hole pad is adopted to make the corpuscles divergent, an exhaust port is arranged on a gun barrel, etc., therefore, the device reduces shock waves and eliminates the contamination of smoke of gunpowder, and the utility model can be suitable for field operation in the state of normal pressure.
Description
The utility model belongs to particulate launching device technical field.Particularly be applied to the particulate launching device in the genetically engineered.
The particulate lift-off technology is applied among the gene engineering technology field, is the particulate with certain momentum to be taken gene invade cell, and obtains stably express, reaches to change the animal and plant proterties, improves or cultivate the purpose of new variety.This technology can once import gene in the hundreds of cell, and efficient height not only, and not limited by acceptor type all is suitable for protoplastis, unicellular, cell mass, tissue block.
1984, the people such as J.C.Sanford of Cornell Univ USA (Cornell University) invented little bullet technology.87 years, they realized the transient state expression of foreign gene in the onion cell with this technology.Its principle of work is described below: be used as power with gunpowder, quicken the nylon bullet.There is an alveole at the front end center of nylon bullet, loads onto the tungsten particle or the goldc grains group that carry gene.The nylon bullet flies out, and knocks block, stop motion, and tungsten particle breaks away from the nylon bullet, continues flight, hits biological target.Realize that the device of above-mentioned working process---particle gun is produced product by E.I.Du Pont Company, its structure as shown in Figure 1, its main body is a vacuum sealing box body (1), gun barrel (2) is equipped with in its upper end, two dividing plates are arranged in the casing, upper spacer (3) is the hollow round table plate, place the synthetic glass block (4) that there is through hole at a center on this round platform, lower clapboard (5) is the middle plate that circular concave station is arranged, biological sample pond (6) is placed on the round platform, the nylon bullet (7) that quickens penetrates from gun barrel, is cut retaining by the synthetic glass block, stop motion, the tungsten particle that carries on the nylon head then breaks away from the nylon bullet, pass the block central small hole, continue flight downwards, hit biological sample.The weak point of this device is: 1. the nylon bullet quickens in gun barrel, can form shockwave, and biological sample is splash; 2. the gunpowder smoke of gunpowder that quickens the nylon bullet can enter vacuum chamber, pollutes biological specimen easily; 3. be not suitable under atmospheric environment, operating, can not be suitable for the needs of field operation.
The purpose of this utility model is to overcome the weak point of said apparatus, design a kind of new bump emission particulate device, reach and to reduce shockwave, can overcome the pollution of the smoke of gunpowder and gunpowder residue again, can under the rough vacuum condition of laboratory, operate, again can be under condition of normal pressure, the purpose of operation in the open air, to satisfy different needs.
The bump emission particulate device that is used for the genetically engineered operation that the utility model provides, formed by parts such as blank fire powder bullet, striker, nylon bullet, gun barrel, stoppers, it is characterized in that also comprising the datum hole pad, there is tubaeform aperture at this datum hole pad center, on the said gun barrel tube wall venting port is arranged, the said gun barrel mouth of pipe inserts in little cartridge stop seat, said stopper is solid boss disk plate, be placed in the said block seat, this block is the plane towards the one side of gun barrel mouth, there is an alveole another side center, and the particulate group of carrying gene is mounted in it.
Principle of work of the present utility model is described below: ignite the blank fire powder bullet by the striker that is placed in the gun barrel; promote the nylon bullet and quicken flight along gun barrel; nylon bullet bump stopper; make the particulate in the stopper another side alveole obtain kinetic energy; break away from stopper; quicken flight, pass the centre hole of datum hole pad, launch and hit biological target.This device that the utility model provides has adopted solid stopper, and the particulate that carries gene separates with the nylon bullet; Adopted the datum hole pad, particulate is dispersed out; The gun barrel sidewall has measures such as vapor pipe, and it is isolated to make that the shockwave that explodes is blocked piece, and the smoke of gunpowder that blast causes and residual gunpowder waste residue are blocked piece to be stopped, discharges by the gun barrel venting port.Because these innovative approachs make this device in use not only reduce shockwave and pollute with avoiding, and can be implemented in work under the normal pressure, have created condition for working in the open air.
Brief Description Of Drawings:
The PDS-1000 particle gun structural representation that Fig. 1 produces for E.I.Du Pont Company.
Fig. 2 is one of the utility model embodiment---the bump emission particulate apparatus structure synoptic diagram under the low vacuum state.
Fig. 3 is two of the utility model embodiment---the hand-held particle gun structural representation of field operation.
A kind of bump emission particulate device embodiment that is used under the low vacuum state that the utility model provides, its structure as shown in Figure 2, by gun barrel (2) and be placed on blank fire powder bullet in the gun barrel, striker (not drawing among the figure) nylon bullet (9), stopper (4), part such as datum hole pad (11) and little cartridge stop seat (10) constitutes, it is characterized in that also comprising vacuum sealed box (1) and be located at the interior biological sample worktable (8) of case, said little cartridge stop seat (10) is the step type sleeve pipe, said gun barrel (2) inserts in this block seat, (stopper is the boss disk to be bottled up muzzle by said stopper (4), its small end is filled in the muzzle, the fossette of its large end face center is placed with the particulate that carries gene), said stopper (4) is by datum hole pad (11), sealing-ring (12) is placed in said little cartridge stop seat (10), and said gun barrel side venting port (13) is arranged on outside this block seat.Said vacuum sealed box (1) tightens together by sealing-ring (14) with the other end of this block seat, and has the little cartridge stop seat of a through hole and this to communicate, and particulate and biological sample on the stopper are among the low vacuum state.
Another embodiment that the utility model provides as shown in Figure 3, for can be used for the hand-held particle gun of field operation, its profile is as a pistol, comprise pikestaff (15), triggering mechanism (16), preset pieces (17), back-up ring (18), outer gun barrel parts such as (19), said little cartridge stop seat (10) is that an end has bell-mouthed tapered pipe, said outer gun barrel (19) cover therein, interior gun barrel (2) overlaps outside in the gun barrel, locate with preset pieces (17), back-up ring (18) is fixing, this block seat sidewall has a through hole (20) to communicate with said gun barrel venting port (13), said stopper (4) one end plugs are gone in the said muzzle, are placed with the particulate group of carrying gene in its center, other end alveole, this stopper (4), datum hole pad (11) is placed in this block seat successively with sealing-ring (12).This structure practicality, portable can be advantageously used in field work.
Claims (3)
1, a kind of bump emission particulate device that is used for the genetically engineered operation, formed by parts such as blank fire powder bullet, striker, nylon bullet, gun barrel, stoppers, it is characterized in that also comprising the datum hole pad, there is tubaeform aperture at this datum hole pad center, on the said gun barrel tube wall venting port is arranged, the said gun barrel mouth of pipe inserts in little cartridge stop seat, said stopper is solid boss disk plate, be placed in the said block seat, this block is the plane towards the one side of gun barrel mouth, there is an alveole another side center, and the particulate group of carrying gene is mounted in it.
2, bump emission particulate device as claimed in claim 1, it is characterized in that also comprising vacuum sealed box (1) and be located at the interior biological sample worktable (8) of case, said little cartridge stop seat (10) is the step type sleeve pipe, said gun barrel (2) inserts in this block seat, (stopper is the boss disk to be bottled up muzzle by said stopper (4), its small end is filled in the muzzle, the fossette of its large end face center is placed with the particulate that carries gene), said stopper (4) is by datum hole pad (11), sealing-ring (12) is placed in said little cartridge stop seat (10), and said gun barrel side venting port (13) is arranged on outside this block seat.Said vacuum sealed box (1) tightens together by sealing-ring (14) with the other end of this block seat, and has the little cartridge stop seat of a through hole and this to communicate.
3, bump emission particulate device as claimed in claim 1, comprise pikestaff (15), triggering mechanism (16), preset pieces (17), back-up ring (18), outer gun barrel parts such as (19), said little cartridge stop seat (10) is that an end has bell-mouthed tapered pipe, said outer gun barrel (19) cover therein, interior gun barrel (2) overlaps outside in the gun barrel, locate with preset pieces (17), back-up ring (18) is fixing, this block seat sidewall has a through hole (20) to communicate with said gun barrel venting port (13), said stopper (4) one end plugs are gone in the said muzzle, be placed with the particulate group of carrying gene in its center, other end alveole, this stopper (4), datum hole pad (10) is placed in this block seat successively with sealing-ring (12).This structure practicality, portable can be advantageously used in field work.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91207467U CN2091865U (en) | 1991-05-09 | 1991-05-09 | Device for shooting corpuscle by impact |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91207467U CN2091865U (en) | 1991-05-09 | 1991-05-09 | Device for shooting corpuscle by impact |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2091865U true CN2091865U (en) | 1992-01-01 |
Family
ID=4916896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN91207467U Pending CN2091865U (en) | 1991-05-09 | 1991-05-09 | Device for shooting corpuscle by impact |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2091865U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079430C (en) * | 1999-12-11 | 2002-02-20 | 周芳 | High-pressure gas gene gun |
| CN108957026A (en) * | 2018-05-23 | 2018-12-07 | 安徽工业大学 | A kind of critical ball rebound velocity measuring device and method of hot fly ash granule |
-
1991
- 1991-05-09 CN CN91207467U patent/CN2091865U/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079430C (en) * | 1999-12-11 | 2002-02-20 | 周芳 | High-pressure gas gene gun |
| CN108957026A (en) * | 2018-05-23 | 2018-12-07 | 安徽工业大学 | A kind of critical ball rebound velocity measuring device and method of hot fly ash granule |
| CN108957026B (en) * | 2018-05-23 | 2020-07-03 | 安徽工业大学 | Device and method for measuring critical rebound velocity of thermal-state fly ash particles |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| AD01 | Patent right deemed abandoned | ||
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |