CN208450048U - Intelligence for detected by Western blot washes film device - Google Patents
Intelligence for detected by Western blot washes film device Download PDFInfo
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- CN208450048U CN208450048U CN201820447632.6U CN201820447632U CN208450048U CN 208450048 U CN208450048 U CN 208450048U CN 201820447632 U CN201820447632 U CN 201820447632U CN 208450048 U CN208450048 U CN 208450048U
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Abstract
Washing film device the utility model relates to a kind of intelligence for detected by Western blot includes base, wave pallet and wiper mechanism, the pallet horizontal location of waving is slidably fitted at the top of base, it is described wave tray bottom be connected with driving wave the swinging drive mechanism that pallet moves back and forth horizontally, the wiper mechanism includes the cleaning box placed for NC film, the drain component for adding the liquid feeding component of PBST solution into cleaning box and PBST solution in cleaning box being discharged, liquid feeding component includes fluid filling pump, the inlet of fluid filling pump is connected with inlet tube, the liquid outlet of fluid filling pump is connected with the liquid pushing tube for leading to cleaning box, drain component includes positive displacement pump, the inlet of positive displacement pump is connected with the drain pipe for leading to cleaning box, the liquid outlet of positive displacement pump is connected with liquid back pipe.Realize that automatic shaking, liquid feeding, tapping completion wash membrane operations automatically, it is time saving and energy saving, improve test efficiency.
Description
Technical field
The utility model relates to laboratory equipments, and detected by Western blot is specifically used in molecular biosciences laboratory
PBST washes the device of film in (Western blotting) experimentation.
Background technique
Detected by Western blot (Western blotting), abbreviation Western Blot is a kind of by high-resolution
The hybridization technique that gel electrophoresis and immunochemistry analytical technology combine.Western blot is big with analysis capacity, susceptibility is high,
The advantages that high specificity is a kind of most common method for detecting protein properties, expression and distribution, qualitative such as tissue antigen
Antibody or antigen detection of quantitative detection, the quality determination of peptide molecule and virus etc..
Western Blot is the experiment of a time-consuming consumption energy, and after electrophoresis and transferring film, albumen is transferred to NC film
(nitrocellulose filter is also known as NC film, and the supporting body of C/T line is used as in colloid gold test paper, while being also the hair of immune response
At raw.) on, destination protein is shown with developing solution after being immunoreacted with primary antibody secondary antibody, in primary antibody secondary antibody immunoreaction process
In, need to carry out repetitious PBST(PBST i.e. PBS+Tween:PBS is phosphate buffered saline solution (phosphate buffer
Saline) generally as solvent, play dissolution protection reagent；Tween is non-ionic detergent, there is the work of renaturation antigen
With.) membrane process is washed, film time and number difference are washed in each laboratory, but operate by being accomplished manually, and need experimenter anti-always
Multiple topples over, time-consuming and laborious.
Utility model content
The utility model purpose of utility model: to overcome defect of the existing technology, the utility model provides a kind of reality
The intelligence for detected by Western blot for now washing membrane operations automatically washes film device, realizes that automatic shaking, liquid feeding, tapping are washed automatically
Membrane operations are time saving and energy saving.
To achieve the above object, the utility model provides following technical solution:
A kind of intelligence for detected by Western blot washes film device, it is characterised in that: including base, wave pallet and
Wiper mechanism, the pallet horizontal location of waving are slidably fitted at the top of base, it is described wave tray bottom and be connected with driving shake
The swinging drive mechanism that moves back and forth horizontally of pendulum pallet, the wiper mechanism include the cleaning box placed for NC film, to cleaning box
The liquid feeding component of interior addition PBS solution and the drain component that PBST solution in cleaning box is discharged, liquid feeding component includes liquid feeding
Pump, the inlet of fluid filling pump are connected with inlet tube, and the liquid outlet of fluid filling pump is connected with the liquid pushing tube for leading to cleaning box, drain component
Including positive displacement pump, the inlet of positive displacement pump is connected with the drain pipe for leading to cleaning box, and the liquid outlet of positive displacement pump is connected with liquid back pipe.
By using above-mentioned technical proposal, NC film is placed in cleaning box, and fluid filling pump is sent into PBST cleaning into cleaning box
Liquid waves pallet and moves back and forth horizontally, to shake cleaning box, after the PBST cleaning solution in cleaning box is arranged through positive displacement pump
Out, it completes to wash film repeatedly；The parameters such as liquid inlet volume, scavenging period, washing times are set as needed；Realize that film is washed in automation
Movement reduces the inconvenience of manual operation bring, time saving and energy saving, while washing in membrane process, nurses always, mentions without special personnel
High test efficiency.
Preferably, the fluid filling pump and positive displacement pump are fixedly mounted in base, and the liquid pushing tube includes being fixedly connected on to shake
The third pipe of the first pipeline section, the second pipeline section and linking the first pipeline section and the second pipeline section that are fixed on the machine seat on pendulum pallet
Section, second pipeline section are connect with fluid filling pump, and the drain pipe includes being fixed on the 4th pipeline section, fixation and the machine waved on pallet
6th pipeline section of the 4th pipeline section and the 5th pipeline section of the 5th pipeline section and linking on seat, the 5th pipeline section are connect with positive displacement pump,
6th pipeline section and third pipeline section are with waving the telescopic structure to stretch when pallet moves back and forth horizontally.Structure design
Under, structure is clean and tidy, is easily installed arrangement.
Preferably, the 6th pipeline section and third pipeline section are the helical form extended consistent with pallet moving direction is waved.It should
Under structure design, structure is simple, and can preferably adapt to wave pallet by a small margin type frequently reciprocating the characteristics of.
Preferably, the pallet that waves is equipped at least two cleaning box, the first pipeline section and the 4th pipeline section include with
Each branch pipe of each one-to-one branch pipe of cleaning box, the first pipeline section is connected with feed liquor collecting pipe, feed liquor confluence through pipe fitting
Pipe is connect with third pipeline section, and each branch pipe of the 4th pipeline section is connected with drain collecting pipe, drain collecting pipe and the 6th through pipe fitting
Pipeline section connection, first pipeline section is equipped with controlling its each branch pipe simultaneously and feed liquor switch groups with third pipeline section connection respectively
Part, the 4th pipeline section is equipped with controlling its each branch pipe simultaneously and the drain changeover module with the 6th pipeline section connection respectively.The structure is set
, it can be achieved that multiple wash membrane operations under meter, test efficiency is improved, meanwhile, multiple branch pipes of feed liquor are picked out through single feed liquor collecting pipe,
Similarly going out liquid branch pipe is also individually to go out liquid collecting pipe to pick out, and preferably adapts to the movement for waving pallet, and reduction waves pallet dynamic
To the influence of pipeline when making, structure is more compact.
Preferably, the cleaning box, which is releaseably placed in, waves on pallet, and it is fixed equipped with cleaning box to wave pallet
Position slot, cleaning box positioning groove bottom wall protrusion are provided with locating convex block, and locating convex block is equipped with drainage hole, and support is waved in drainage hole perforation
Pan bottom, drainage hole are connect with drain pipe, and the cleaning box bottom is equipped with the recess with locating convex block corresponding matching, recess
Place is equipped with the liquid outlet connector mating with drainage hole, and liquid outlet connector is shunk in recess.Under structure design, cleaning box can be taken out
Pallet is waved, convenient for the operation that is removed and placed into of NC film, locating convex block, drainage hole, recess and liquid outlet connector cooperation, so that clearly
Positioning and the connection for washing box are reliable, and drainage hole is set to and waves tray bottom, convenient for drain and has the advantages that compact-sized.
Preferably, anti-drip valve is equipped in the liquid outlet connector, anti-drip valve includes conical valve seat mouth, the cooperation of conical valve seat mouth
Spool is blocked, spool is blocked and press is cooperated to block the compressed spring that spool tends to closing conical valve seat mouth, conical valve seat mouth top
With the web plate pressed for compressed spring, mesh sheet is equipped in drainage hole, mesh sheet center, which is equipped with, is plugged in drain in liquid outlet connector
Pushing tow closure spool overcomes compressed spring to be detached from the thimble of conical valve seat mouth behind hole.It is de- in liquid outlet connector under structure design
After the fluid apertures that begins to rehearse, under compressed spring effect anti-drip valve is closed, reach leakproof, when washing membrane operations, liquid outlet connector insertion
In drainage hole, thimble pushing tow blocks spool and realizes opening, it can be achieved that normally washing membrane operations.
Preferably, the swinging drive mechanism includes driving motor, eccentric driving and rocker arm, the fixed peace of driving motor
Loaded on base, on the output shaft of driving motor, one end bias is articulated in eccentric driving for eccentric driving installation,
The rocker arm other end is articulated in the bottom for waving pallet.Under structure design, swinging drive mechanism structure is simple, realizes fast by a small margin
Speed is shaken, and membrane efficiency height is washed.
The utility model is further described with reference to the accompanying drawing.
Detailed description of the invention
Fig. 1 is that intelligence of the utility model concrete example for detected by Western blot washes film device schematic perspective view；
Fig. 2 is that the utility model concrete example washes the inside after film device removal base for the intelligence of detected by Western blot
Structural schematic diagram one；
Fig. 3 is that the utility model concrete example washes the inside after film device removal base for the intelligence of detected by Western blot
Structural schematic diagram two；
Fig. 4 the utility model concrete example washes the structure after film device removes cleaning box for the intelligence of detected by Western blot
Schematic diagram；
Fig. 5 is the structural schematic diagram of the utility model concrete example cleaning box；
Fig. 6 is the structural schematic diagram of the utility model concrete example anti-drip valve and its fit structure.
Specific embodiment
Referring to attached drawing 1 ~ 6, a kind of intelligence for detected by Western blot disclosed by the utility model washes film device, wraps
It includes base 1, wave pallet 2 and wiper mechanism, it is described to wave pallet 2 and be slidably fitted in base through 3 horizontal location of guide rail mechanism
Top, guide rail mechanism 3 are there are the prior art of multiple structural forms, and so it will not be repeated, and 2 bottom of pallet of waving is connected with
The swinging drive mechanism 4 that pallet 2 moves back and forth horizontally is waved in driving, the wiper mechanism include the cleaning box 5 placed for NC film,
The drain component for adding the liquid feeding component of PBST solution into cleaning box 5 and PBST solution in cleaning box 5 being discharged, liquid feeding group
Part includes fluid filling pump 61, and the inlet of fluid filling pump 61 is connected with inlet tube 62, and the liquid outlet of fluid filling pump 61, which is connected with, leads to cleaning
The liquid pushing tube 63 of box 5, drain component include positive displacement pump 71, and the inlet of positive displacement pump 71 is connected with the drain pipe for leading to cleaning box 5
72, the liquid outlet of positive displacement pump 71 is connected with liquid back pipe 73.NC film is placed in cleaning box, and fluid filling pump is sent into PBST into cleaning box
Cleaning solution waves pallet and moves back and forth horizontally, to shake cleaning box, after through positive displacement pump by the PBST cleaning solution in cleaning box
Discharge is completed to wash film repeatedly；The parameters such as liquid inlet volume, scavenging period, washing times are set as needed；Realize that automation is washed
Film movement reduces the inconvenience of manual operation bring, time saving and energy saving, while washing in membrane process, nurses always without special personnel,
Improve test efficiency.As the conventional design of electronic field, the liquid crystal display 11 or operation panel of human-computer interaction can be equipped on base,
Realize the parameter settings such as liquid inlet volume, scavenging period, washing times.
Wherein, cleaning box can be waved directly directly to process on pallet and be formed, and operated and processed for convenience certainly, described clear
It washes box 5 and is releaseably placed in and wave on pallet 2, cleaning box 5 is independent accessory, and cleaning is equipped on waving pallet 2
Box locating slot 21,21 bottom wall protrusion of cleaning box locating slot are provided with locating convex block 211, and locating convex block 211 is equipped with drainage hole
212,2 bottom of pallet is waved in the perforation of drainage hole 212, and drainage hole 212 is connect with drain pipe 72, and 5 bottom of cleaning box is set
There is the recess 51 with 211 corresponding matching of locating convex block, be equipped with the liquid outlet connector 52 mating with drainage hole 212 at recess 51,
Liquid outlet connector 52 is shunk in recess 51.Cleaning box can be taken out and wave pallet, be removed and placed into operation, convex convenient for NC film
Block, drainage hole, recess and liquid outlet connector cooperation, so that the positioning and connection of cleaning box are reliable, drainage hole is set to and waves support
Pan bottom, convenient for the arrangement of drain and pipeline, so that compact-sized.And in order to enable cleaning box disengages when waving pallet, out
Phenomenon to be dripped outside the residual liquid of liquid joint, anti-drip valve is equipped in liquid outlet connector 52, anti-drip valve includes conical valve seat mouth 521,
The cooperation of conical valve seat mouth 521 blocks spool 522, and the closure cooperation press of spool 522 blocks spool 522 and tends to close conical valve seat mouth
521 compressed spring 523, conical valve seat mouth top have the web plate 524 pressed for compressed spring, are equipped in drainage hole 212
Mesh sheet 213,213 center of mesh sheet are equipped with the pushing tow closure spool 522 after liquid outlet connector 52 is plugged in drainage hole 212 and overcome compression bullet
Spring 524 is made under compressed spring effect after liquid outlet connector disengages drainage hole with the thimble 214 for being detached from conical valve seat mouth 521
Anti-drip valve is closed, and leakproof is reached, and when washing membrane operations, liquid outlet connector is inserted into drainage hole, and thimble pushing tow blocks spool realization and beats
It opens, it can be achieved that normally washing membrane operations, close-fitting or buckle structure etc. realization can be used in cleaning box and the connection for waving pallet.
Liquid pushing tube and cleaning box are also that disengagable formula structure designs, and drain pipe and cleaning box can also be used in the cooperation of liquid pushing tube and cleaning box
Fit structure form；It is liquid pushing tube when the pipe end of liquid pushing tube can also be used setting pipe end joint 63-1(more a cleaning box design
Pipe end joint is set on branch pipe pipe end), the pipe end joint connector connected for pipe end joint 631 is directly set on cleaning box
53, using pipe end joint connector is arranged on the box cover of cleaning box in figure.Cleaning box generally includes box body and box cover, box body and
Realize that (buckle structure of buckle structure omission in figure, box cover and box body is existing skill for connection using buckle structure between box cover
Art).
The fluid filling pump 61 and positive displacement pump 71 are fixedly mounted in base 1, and the mounting structure of pump is routine techniques, therefore no longer
It repeats, the liquid pushing tube 63 includes being fixedly connected on the first pipeline section 631 waved on pallet 2, second be fixed on base 1 pipe
Section 632 and the third pipeline section 633 for being connected the first pipeline section 631 and the second pipeline section 632, second pipeline section 632 and fluid filling pump 61
Connection, the drain pipe 72 include being fixed on the 4th pipeline section 721 waved on pallet 2, fixed the 5th pipeline section on base 1
722 and it is connected the 6th pipeline section 723 of the 4th pipeline section 721 and the 5th pipeline section 722, the 5th pipeline section 723 connects with positive displacement pump 71
Connect, the 6th pipeline section 723 and third pipeline section 633 be with wave pallet 2 it is mobile back and forth horizontally when flexible telescopic structure.Institute
It states the 6th pipeline section 723 and third pipeline section 633 is the helical form extended consistent with 2 moving direction of pallet is waved.Structure is simple, and energy
Preferably adapt to wave pallet by a small margin type frequently reciprocating the characteristics of.
In this specific embodiment, the pallet 2 that waves is equipped at least two cleaning box 5, the first pipeline section 631 and the 4th
Pipeline section 721 includes and each cleaning box 5 one-to-one branch pipe 631-1,721-1, each branch pipe 631- of the first pipeline section 631
1 is connected with feed liquor collecting pipe 631-2 through pipe fitting, and feed liquor collecting pipe 631-2 is connect with third pipeline section 633, the 4th pipeline section 721
Each branch pipe 721-1 is connected with drain collecting pipe 721-2 through pipe fitting, and drain collecting pipe 721-2 is connect with the 6th pipeline section 723,
First pipeline section 631 is equipped with controlling its each branch pipe simultaneously and the feed liquor changeover module with 633 connection of third pipeline section respectively, and the
Four pipeline sections 721 are equipped with controlling its each branch pipe simultaneously and the drain changeover module with 723 connection of the 6th pipeline section respectively.Wherein feed liquor
The shut-off valve on each branch pipe can be used in changeover module and drain changeover module, can be used certainly drain collecting pipe and into
Four position three-way valves can be used as used under two cleaning box designs in this specific embodiment in solenoid directional control valve on liquid collecting pipe
Design designs four position three-way valve 631-3 of feed liquor at feed liquor collecting pipe 631-2, and drain four is designed at drain collecting pipe 721-2
Position three-way valve 721-3 realizes that controlling each cleaning box simultaneously or separately connect with fluid filling pump, and each cleaning box of control is simultaneously
Or connect respectively with positive displacement pump, using two designs in figure, structure design is lower, it can be achieved that multiple wash membrane operations, raising test
Efficiency, meanwhile, multiple branch pipes of feed liquor are picked out through single feed liquor collecting pipe, and similarly liquid branch pipe is also individually to go out liquid collecting pipe to connect out
Out, the movement for preferably adapting to wave pallet, reduces the influence for waving pallet in movement to pipeline, and structure is more compact.
In this specific embodiment, the swinging drive mechanism 4 includes driving motor 41, eccentric driving 42 and rocker arm
43, driving motor 41 is fixedly installed on base 1, on the output shaft of the installation of eccentric driving 42 and driving motor 41, Rocker arm 43
One end bias is articulated in eccentric driving 42, and 3 other end of Rocker arm 4 is articulated in the bottom for waving pallet 2.Swinging drive mechanism knot
Structure is simple, realizes and quickly shakes by a small margin, washes membrane efficiency height.
Claims (7)
1. a kind of intelligence for detected by Western blot washes film device, it is characterised in that: including base, wave pallet and clear
Washing machine structure, the pallet horizontal location of waving are slidably fitted at the top of base, it is described wave tray bottom and be connected with driving wave
The swinging drive mechanism that pallet moves back and forth horizontally, the wiper mechanism include the cleaning box placed for NC film, into cleaning box
The drain component for adding the liquid feeding component of PBST solution and PBST solution in cleaning box being discharged, liquid feeding component includes liquid feeding
Pump, the inlet of fluid filling pump are connected with inlet tube, and the liquid outlet of fluid filling pump is connected with the liquid pushing tube for leading to cleaning box, drain component
Including positive displacement pump, the inlet of positive displacement pump is connected with the drain pipe for leading to cleaning box, and the liquid outlet of positive displacement pump is connected with liquid back pipe.
2. washing film device for the intelligence of detected by Western blot according to claim 1, it is characterised in that: the fluid filling pump
It is fixedly mounted in base with positive displacement pump, the liquid pushing tube includes being fixedly connected on the first pipeline section waved on pallet, being fixed on
The third pipeline section of the second pipeline section and linking the first pipeline section and the second pipeline section on base, second pipeline section and fluid filling pump connect
It connects, the drain pipe includes being fixed on the 4th pipeline section waved on pallet, fixation and the 5th pipeline section on base and being connected
6th pipeline section of four pipeline sections and the 5th pipeline section, the 5th pipeline section are connect with positive displacement pump, and the 6th pipeline section and third pipeline section are
With wave when pallet moves back and forth horizontally stretch telescopic structure.
3. washing film device for the intelligence of detected by Western blot according to claim 2, it is characterised in that: the described 6th
Pipeline section and third pipeline section are the helical form extended consistent with pallet moving direction is waved.
4. the intelligence according to Claims 2 or 3 for detected by Western blot washes film device, it is characterised in that: described to shake
It puts pallet and is equipped at least two cleaning box, the first pipeline section and the 4th pipeline section include propping up correspondingly with each cleaning box
Pipe, each branch pipe of the first pipeline section are connected with feed liquor collecting pipe through pipe fitting, and feed liquor collecting pipe is connect with third pipeline section, the 4th pipe
Each branch pipe of section is connected with drain collecting pipe through pipe fitting, and drain collecting pipe is connect with the 6th pipeline section, and first pipeline section is matched
There is its each branch pipe of control while and the feed liquor changeover module with third pipeline section connection, the 4th pipeline section are each equipped with its is controlled respectively
Branch pipe is simultaneously and the drain changeover module with the 6th pipeline section connection respectively.
5. according to claim 1 or 2 or 3 intelligence for detected by Western blot wash film device, it is characterised in that: described
Cleaning box is releaseably placed in and waves on pallet, waves pallet equipped with cleaning box locating slot, cleaning box locating slot bottom
Wall protrusion is provided with locating convex block, and locating convex block is equipped with drainage hole, and tray bottom, drainage hole and drain are waved in drainage hole perforation
Pipe connection, the cleaning box bottom are equipped with the recess with locating convex block corresponding matching, and indent is equipped with and drainage hole grafting
The liquid outlet connector of cooperation, liquid outlet connector are shunk in recess.
6. washing film device for the intelligence of detected by Western blot according to claim 5, it is characterised in that: in the liquid out
Anti-drip valve is equipped in connector, anti-drip valve includes conical valve seat mouth, and the cooperation of conical valve seat mouth blocks spool, blocks spool cooperation press
The compressed spring that spool tends to closing conical valve seat mouth is blocked, there is the web plate pressed for compressed spring above conical valve seat mouth,
Mesh sheet is equipped in drainage hole, mesh sheet center is equipped with the pushing tow closure spool after liquid outlet connector is plugged in drainage hole and overcomes compression bullet
Spring is to be detached from the thimble of conical valve seat mouth.
7. according to claim 1 or 2 or 3 intelligence for detected by Western blot wash film device, it is characterised in that: described
Swinging drive mechanism includes driving motor, eccentric driving and rocker arm, and driving motor is fixedly installed on base, eccentric drive
Disk installation is on the output shaft of driving motor, and one end bias is articulated in eccentric driving, and the rocker arm other end, which is articulated in, to be shaken
Put the bottom of pallet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820447632.6U CN208450048U (en) | 2018-03-30 | 2018-03-30 | Intelligence for detected by Western blot washes film device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820447632.6U CN208450048U (en) | 2018-03-30 | 2018-03-30 | Intelligence for detected by Western blot washes film device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208450048U true CN208450048U (en) | 2019-02-01 |
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ID=65156795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820447632.6U Active CN208450048U (en) | 2018-03-30 | 2018-03-30 | Intelligence for detected by Western blot washes film device |
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| CN (1) | CN208450048U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108453085A (en) * | 2018-03-30 | 2018-08-28 | 倪华静 | Western Blot intelligently wash film device |
| CN110221057A (en) * | 2019-07-05 | 2019-09-10 | 中国医科大学附属盛京医院 | A kind of automatic washing film device |
-
2018
- 2018-03-30 CN CN201820447632.6U patent/CN208450048U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108453085A (en) * | 2018-03-30 | 2018-08-28 | 倪华静 | Western Blot intelligently wash film device |
| CN110221057A (en) * | 2019-07-05 | 2019-09-10 | 中国医科大学附属盛京医院 | A kind of automatic washing film device |
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