CN205797240U - A kind of integrated micro-flow control chip - Google Patents
A kind of integrated micro-flow control chip Download PDFInfo
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- CN205797240U CN205797240U CN201620455263.6U CN201620455263U CN205797240U CN 205797240 U CN205797240 U CN 205797240U CN 201620455263 U CN201620455263 U CN 201620455263U CN 205797240 U CN205797240 U CN 205797240U
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Abstract
This utility model relates to a kind of integrated micro-flow control chip, at least one integrated reaction unit it is provided with on its substrate, and described integrated reaction unit at least includes the sample cell, mixing pit and the reaction tank that are connected by fluid passage, one end of described sample cell is provided with sample inlet, also include gas circuit internal circulation system, one end of described gas circuit internal circulation system is connected with described mixing pit, and the other end at least includes the first circulation branch road being connected with described sample cell away from one end of described sample inlet.This integrated micro-flow control chip ensure that the air pressure balance in sample cell and mixing pit, the most also ensures that sample smooth flow in the pre-routing of micro-fluidic chip, generates target product for sample and provides reliable basis.
Description
Technical field
This utility model relates to biology, medical test, chemical analysis field, particularly relates to a kind of integrated micro-flow control core
Sheet.
Background technology
Microfluidic chip technology is prepared by sample biological, that iatrochemistry analyzes process, to react, separate and detection etc.
Basic operation unit is integrated on the chip of one piece of micro-meter scale, is automatically performed analysis overall process.
Micro-fluidic chip is the main platform that microflow control technique realizes, and its device characteristic is mainly it and accommodates the effective of fluid
Structure (passage, reative cell and other functional parts) is at least micron order yardstick on a latitude, and micro-fluidic chip has liquid
The multiple advantages such as body flowing is controlled, consume sample and reagent is few, analysis speed is fast.
It is usually provided with sample cell, mixing pit and reaction tank on micro-fluidic chip, is connected by microchannel each other,
Outside corollary equipment provides power to make sample be sequentially entered in mixing pit and reaction tank by sample cell, to realize the life of target product
Become.
But, find in actual use, after being compressed due to the residual air in passage, air pressure raises, and causes sample
Sample in product pond cannot be circulated to inside mixing pit the most smoothly, and the liquid within mixing pit also cannot circulate the most smoothly
To reaction tank, the sample flow in whole micro-fluidic chip is extremely difficult.
Therefore, sample how can be made to flow in the pre-routing of micro-fluidic chip smoothly, so that sample generates target
Product is that current those skilled in the art need badly and solve the technical problem that.
Utility model content
The purpose of this utility model is to provide a kind of integrated micro-flow control chip, thus ensures that sample is smoothly at micro-fluidic core
Flowing in the pre-routing of sheet, and finally make sample generate target product.
For solve above-mentioned technical problem, this utility model provide integrated micro-flow control chip, its substrate is provided with to
A few integrated reaction unit, and described integrated reaction unit at least include being connected by fluid passage sample cell, mixing pit and
Reaction tank, one end of described sample cell is provided with sample inlet, also includes gas circuit internal circulation system, described gas circuit internal circulation system
One end be connected with described mixing pit, the other end at least includes being connected away from one end of described sample inlet with described sample cell
First circulation branch road.
Preferably, described first circulation branch road is further opened with steam vent to be opened/closed, and described gas circuit internal circulation system
It is additionally provided with the second liquid blocking-up structure for blocking liquid near the position of described mixing pit.
Preferably, described integrated micro-flow control chip is the centrifugal type microfludic chip with center of rotation, described sample
Pond, mixing pit and described reaction tank increase successively with the distance of described center of rotation.
Preferably, the Buffer Pool being arranged between described reaction tank and described mixing pit is also included.
Preferably, described reaction tank includes multiple, and described reaction tank is the most equal and described with the distance of described center of rotation
Mixing pit and described reaction tank are by distribution channel connection.
Preferably, described distribution passage is wavy, and its crest is near described center of rotation, and trough is away from described rotation
Center, described reaction tank connects with the wave trough position of described distribution passage, and described mixing pit connects with the head end of described distribution passage
Logical.
Preferably, the other end of described gas circuit internal circulation system also includes second connected with the end of described distribution passage
Circulation branch road.
Preferably, described gas circuit internal circulation system is referred to as the first junction point, described gas with the junction point of described mixing pit (2)
Described first circulation branch road and the junction point of described second circulation branch road in the internal circulation system of road are referred to as the second junction point, and institute
State first junction point distance with described center of rotation more than described second junction point and the distance of described center of rotation.
Preferably, adjacent with the head end of described distribution passage and end trough is connected to a waste liquid pool.
Preferably, described circulation branch road is additionally provided with first liquid blocking-up structure.
Preferably, described first liquid blocking-up structure is the first dilatation chamber of volume discontinuities.
Preferably, described gas circuit internal circulation system be additionally provided with near the position of described mixing pit for block liquid
Two liquid barrier structures.
Preferably, described second liquid blocks the second dilatation chamber that mechanism is volume discontinuities.
Preferably, described liquid barrier structure uses hydrophobic material to make, or is coated with thin in described liquid barrier structure
Water layer.
Preferably, also include being connected with described reaction tank, and with the distance of described center of rotation more than described reaction tank
Sedimentation tank.
Preferably, described mixing pit includes:
It is arranged on the sleeve in described substrate one side；
Run through the two sides of described substrate, and at least two micropore connected with described sleeve inner；
It is embedded in described sleeve, and the rotor coordinated away from the end face of described substrate with described sleeve, on described rotor
It is provided with the plug for blocking and open any one of micropore.
Preferably, described sample cell includes the multiple of parallel connection, and described sample cell all connects with described mixing pit.
Preferably, described mixing pit includes multiple, and described mixing pit is serially connected.
Preferably, described gas circuit internal circulation system is mixing pit steam vent with the junction point of described mixing pit, described mixing
The sample ostium in pond is mixing pit inlet opening, and described mixing pit inlet opening mixes more than described with the distance of described center of rotation
Pond steam vent and the distance of described center of rotation.
Preferably, also include that one end is connected with described first circulation branch road, the buffering that the other end is connected with described sample cell
District.
Preferably, it is embedded with required reaction reagent in described sample cell, mixing pit and reaction tank.
Preferably, described substrate material is the mixture of one or more in glass, silicon, metal, polymer.
Preferably, described substrate and the cover plate that coordinates with it use hot pressing, gluing, laser welder, ultra-sonic welded, screw thread to twist
A kind of mode in Jin encapsulates.
By in above technical scheme it can be seen that in integrated micro-flow control chip disclosed in this utility model, substrate
On be provided with at least one integrated reaction unit, and integrated reaction unit at least include connection sample cell, mixing pit and
Reaction tank, in addition, is additionally provided with gas path circulatory system, and one end of this gas path circulatory system is connected with mixing pit, the other end
At least include the first circulation branch road being connected with sample cell.
It can be appreciated that when adding sample to the sample inlet of sample cell, the air in sample cell would first, through liquid
Passage is driven to mixing pit, uses less speed to be centrifuged, and the liquid in sample cell gradually shifts in mixing pit, this
Time sample cell in the region that originally taken by fluid sample vacancy occurs, the air pressure in sample cell will reduce, and previously transferred to
Along with the entrance of liquid, pressure raises gas in mixing pit, owing to gas circuit internal circulation system one end connects mixing pit, another
End connection sample cell, therefore the gas in mixing pit comes back to sample cell along gas circuit internal circulation system under the effect of pressure reduction
In, this guarantees the air pressure balance in sample cell and mixing pit, it is ensured that sample is under less centrifugal force, at micro-fluidic core
Smooth flow in the pre-routing of sheet, it is to avoid under bigger centrifugal force, quickly mixing pit entrance is rushed by the sample of flowing
Hit, generate target product for sample and provide reliable basis.In like manner, distribution passage, distribution are flowed at sample through mixing pit
Air in pond enters mixing pit via gas circuit internal circulation system, it is ensured that mixed sample is filled up completely with all of distribution and leads to
Road, it is to avoid under high speed centrifugation, mixed sample has preferentially entered equipped with not in the case of being full of distribution passage
Pollute with the reaction tank of reactant.
Accompanying drawing explanation
Fig. 1 is that the structure of the integrated reaction unit of the integrated microcontroller chip disclosed in a kind of embodiment of this utility model is shown
It is intended to；
Fig. 2 is the structural representation of integrated microcontroller chip disclosed in a kind of embodiment of this utility model；
Fig. 3 is the structural representation of integrated microcontroller chip disclosed in another embodiment of this utility model；
Fig. 4 is the structural representation of integrated microcontroller chip disclosed in this utility model another embodiment；
Fig. 5 is the structural representation of mixing pit disclosed in this utility model embodiment；
Fig. 6 is the schematic top plan view of mixing pit disclosed in this utility model embodiment；
Fig. 7 is the structural representation of the integrated microcontroller chip of the multiple mixing pits series connection disclosed in this utility model embodiment
Figure；
Fig. 8 is the structural representation of the integrated microcontroller chip of the multiple sample cells parallel connection disclosed in this utility model embodiment
Figure.
Wherein, 1 is sample cell, and 2 is mixing pit, and 3 is reaction tank, and 4 is sample inlet, and 51 is first segment, and 52 is second
Sections, 53 is the 3rd sections, and 54 is steam vent, and 6 is fluid passage, and 7 is the second dilatation chamber, and 8 is the first dilatation chamber, and 9 is second
Circulation branch road, 10 is distribution passage, and 11 is Buffer Pool, and 12 is sedimentation tank, and 13 is waste liquid pool, and 14 is mixing pit inlet opening, and 15 are
Mixing pit steam vent, 16 is substrate, and 17 is chip tray, and 18 is sleeve, and 19 is rotor, and 20 is micropore, and 21 is plug, and 22 is micro-
Pipeline.
Detailed description of the invention
Core of the present utility model is to provide a kind of integrated micro-flow control chip, in order to ensure that sample is smoothly at micro-fluidic core
Flowing in the pre-routing of sheet, and finally make sample generate target product.
In order to make those skilled in the art be more fully understood that this utility model scheme, below in conjunction with the accompanying drawings and be embodied as
The utility model is described in further detail for mode.
In integrated micro-flow control chip disclosed in the utility model, its substrate 16 is provided with at least one integrated reaction
Unit, at least includes sample cell 1, mixing pit 2 and reaction tank 3 in this integrated reaction unit, wherein sample cell 1 is for sample
Temporary, mixing pit 2 is that reaction tank 3 is sample for needing the material carrying out mixing to carry out the place mixed with other in sample
Finally reacting and generate the place of target product, this three is connected by fluid passage, as shown in fig. 1, and sample
Pond 1, in strip, is followed in being additionally provided with gas circuit in one end of sample cell 1 is provided with sample inlet 4, and this micro-fluidic chip
Loop systems, one end of gas circuit internal circulation system is connected with mixing pit 2, and the other end at least includes with sample cell 1 away from sample inlet 4
One end be connected first circulation branch road.
Refer to Fig. 1, when adding sample to the sample inlet 4 of sample cell 1, the air in sample cell 1 would first, through
Fluid passage is driven to mixing pit 2, then by the corollary equipment coordinated with chip, the liquid in sample cell 1 gradually to
Transfer in mixing pit 2, there is vacancy in the region now originally taken by fluid sample in sample cell 1, and the air pressure in sample cell 1 will
Can reduce, pressure raises, due to gas circuit internal circulation system one along with the entrance of liquid previously to have transferred to gas in mixing pit 2
End connection mixing pit 2, other end connection sample cell 1, therefore the gas in mixing pit 2 follows under the effect of pressure reduction in gas circuit
Loop systems comes back in sample cell 1, this guarantees the air pressure balance in sample cell 1 and mixing pit 2, it is ensured that relatively low
Under rotating speed, sample in the pre-routing of micro-fluidic chip can smooth flow, reduce the sample impact to mixing pit injection port, for
Sample generates target product and provides reliable basis.
Further, in order to avoid (gas in sample cell 1, mixing pit 2 and both connecting pipes when adding sample
Road circulation canal and fluid passage) retain too much gas, also offering on above-mentioned first circulation branch road in the present embodiment can
The steam vent 54 of opening and closing.When adding sample in sample cell, steam vent 54 is also opened, and after sample-adding, sample enters
Mouth 4 and steam vent 54 all will be closed, to avoid the leakage of sample.
In order to avoid the liquid in mixing pit flows in gas circuit internal circulation system, gas circuit internal circulation system also include close
The second liquid blocking-up structure for blocking liquid that the position of mixing pit is arranged, as shown in fig. 1, this liquid barrier structure can
Think the second dilatation chamber of volume discontinuities.
Refer to Fig. 1, the first circulation branch road in the present embodiment is specifically formed by two sections, the respectively second sections 52 and the
Three sections 53, wherein, first segment 51 is drawn by mixing pit 2 as one end of gas circuit internal circulation system, and the 3rd sections 53 is by sample
Product pond 1 is drawn, and second stage 52 is to connect first segment 51 and the interlude of the 3rd sections 53.And first segment 51 and the 3rd
The passage section of sections 53 is less than the second sections, to avoid the liquid in sample cell 1 and mixing pit 2 to circulate in entering into gas circuit
In system.
Further, in order to avoid sample is flowed in gas circuit internal circulation system by mixing pit 2, gas circuit internal circulation system leans on
The position of nearly mixing pit 2 is additionally provided with the second liquid blocking-up structure for blocking liquid, and blocking-up structure can be by arranging
Valve body realizes, but the most complex, uses volume to become suddenly for the second liquid blocking-up structure in this present embodiment
The the second dilatation chamber 7 changed realizes, and the pipeline that area changes suddenly can form the effective obstruct to liquid stream, thus avoids liquid-like
Product enter in gas circuit internal circulation system.
Those skilled in the art know, and micro-fluidic chip needs to coordinate with corollary equipment when actually used, corollary equipment
Both can be that the flowing of sample provides power in micro-fluidic chip, it is possible to provide dynamic for the motion of other microcomponents in micro-fluidic chip
Power.Therefore, the sample in micro-fluidic chip (abbreviation of Integrated microfluidic chip) can have multiple different type of drive, example
Such as the gas push sample flow provided by corollary equipment, or by corollary equipment, micro-fluidic chip is rotated, from
And by the flowing of centrifugal force pulls sample.Integrated micro-flow control core in order to simplify corollary equipment, in this utility model embodiment
Sheet is the centrifugal type microfludic chip with center of rotation, accordingly, in order to realize sample under the influence of centrifugal force by sample
Flow successively in mixing pit 2, reaction tank 3 in pond 1, also need to ensure sample cell 1, mixing pit 2 and reaction tank 3 and center of rotation away from
From increasing successively.
Needing to illustrate, the center of rotation in above-described embodiment may be located on the substrate 16 of micro-fluidic chip,
May be alternatively located at outside micro-fluidic chip, because in actual centrifugal process, micro-fluidic chip is frequently placed at chip tray 17
On, therefore center of rotation may be alternatively located on chip tray 17.
As shown in Fig. 2 to Fig. 4, a micro-fluidic chip can contain only an integrated reaction unit, it is also possible to
The multiple integrated reaction unit of arrangement (in Fig. 3 being 4) in one micro-fluidic chip, it is, of course, also possible at a chip tray 17
It is embedded on each micro-fluidic chip in multiple micro-fluidic chip (in Fig. 4 being 2) embedded core tablet tray 17 and can also arrange
One or more integrated reaction unit.
Further, relief area also can be set between the first circulation branch road and sample cell 1, in order to micro-fluidic chip
Interior sample-adding or carry out playing cushioning effect, certainly during other operations, it is possible to do not set between sample cell 1 and the first circulation branch road
Put special relief area, only the first circulation branch road is joined directly together with sample cell 1.
As shown in fig. 1, in order to avoid producing bigger impact, time in sample entrance reaction tank 3 in the present embodiment preferably
Buffer Pool 11 is set between reaction tank 3 and mixing pit 2, to ensure that sample can enter in reaction tank 3 with unhurried current state.
According to different needs, reaction tank 3 can be single or multiple, when reaction tank 3 is multiple, and multiple reaction tanks
3 should be equal with the distance of center of rotation, and is connected by distribution passage 10 between mixing pit 2 with multiple reaction tanks 3, such as figure
Shown in 1 to Fig. 4, Fig. 7 to Fig. 8, distribution passage 10 disclosed in this utility model embodiment is specifically in wavy, and this is wavy
The crest of distribution passage 10 is near center of rotation, and trough is away from center of rotation, reaction tank 3 and the wave trough position phase distributing passage 10
Connection, mixing pit 2 connects with the head end of distribution passage 10.
Need to illustrate, owing to the overall bearing of trend of distribution passage 10 is the circumference along micro-fluidic chip, because of
Any one end of this distribution passage 10 all can be as its head end, and its other end is tail end, in actual design process, and mixing pit
2 only need with near self closer to distribution passage 10 one end be connected, accordingly, this end be distribute passage 10 head end.
As an alternative, when reaction tank 3 includes multiple, the trough adjacent with distribution passage 10 head end and end can divide
Not Lian Jie a waste liquid pool 13, the volume of this waste liquid pool 13 is 0.5-10 times of each reaction tank 3, after being arranged such, close
The waste liquid pool 13 of mixing pit 2 may be used for accommodating the remaining liq in mixing pit 2, and the waste liquid pool 13 away from mixing pit 2 can be used
Liquid unnecessary within accommodating distribution passage 10.
As shown in fig. 1, the other end of gas path circulatory system also includes the second circulation being connected with distribution passage 10 end
Branch road 9.This second circulation branch road 9 furthermore achieved that the interior circulation of gas in distribution passage 10.Concrete, at sample to distribution
In passage 10 during flowing, first the air in distribution passage 10 will be driven to the second circulation branch road 9, then set by supporting
Standby, the liquid in mixing pit 2 gradually shifts in distribution passage 10, the district now originally taken by fluid sample in mixing pit 2
Territory occurs that vacancy, the air pressure in mixing pit 2 will reduce, and has previously transferred to the gas in the second circulation branch road 9 along with liquid
Entering and pressure rising, owing to connecting with gas circuit internal circulation system, therefore the gas in the second circulation branch road 9 is in the effect of pressure reduction
Under first segment 51 in gas circuit internal circulation system enter in mixing pit 2, this guarantees mixing pit 2 and distribution passage 10
Interior air pressure balance, further ensures sample and is completely filled with distributing passage, flow smoothly in the pre-routing of micro-fluidic chip
Dynamic.
One end that cross-sectional area gas circuit the to be noticeably greater than inner cycle channel of the second circulation branch road 9 is connected with mixing pit 2,
The i.e. cross-sectional area of first segment 51.Further, the second circulation branch road 9 is flowed in order to avoid sample by distributing passage 10
In, the second circulation branch road 9 is additionally provided with the first liquid blocking-up structure for blocking liquid, blocking-up structure can be by arranging
Valve body realizes, but the most complex, uses volume to become suddenly for the first liquid blocking-up structure in this present embodiment
The the first dilatation chamber changed realizes 8, and the pipeline that area changes suddenly can form the effective obstruct to liquid stream, thus avoids liquid-like
Product enter in circulation branch road.
In order to improve liquid barrier effect, sample cell 1, mixing pit 2, distribution passage 10 and the connection pipeline of reaction tank 3
And the inner surface of pipeline of gas circuit internal circulation system also can use hydrophobic material to make, owing to hydrophobic material is known in this area
General knowledge, thus at this, hydrophobic material is not done too much introduction, especially first liquid blocking-up structure and second liquid blocking-up structure,
Both use hydrophobic material to make, or coat the liquid barrier effect that hydrophobic material can reach ideal on inwall.
As shown in fig. 1, gas circuit internal circulation system is referred to as the first junction point with the junction point of mixing pit 2, circulation in gas circuit
System is referred to as the second junction point with the junction point of the first circulation branch road and the second circulation branch road 9, and the first junction point with rotate
The distance of the heart is more than the distance of the second junction point with center of rotation.This kind of design makes gas circuit internal circulation system present eccentric shape
State, this liquid that can be prevented effectively from mixing pit 2 enters in gas circuit internal circulation system, it is ensured that gas circuit internal circulation system smooth
Logical.
As shown in fig. 1, also sedimentation tank 12, sedimentation tank 12 and the distance of center of rotation can be set at the end of reaction tank 3
More than the distance of reaction tank 3 to center of rotation, sedimentation tank 12 is the solid content of solution in collecting reaction tank 3, sedimentation tank 12
Volume is 0.05-1 times of reaction tank 3 volume.
Refer to the structural representation that Fig. 5, Fig. 5 are mixing pit disclosed in this utility model embodiment, mixing pit 2 wraps
Including sleeve 18, micropore 20 and rotor 19, its middle sleeve 18 is raised in the one side of substrate 16, and micropore 20 at least includes two, and
Micropore 20 runs through the two sides of substrate 16, connects internal for sleeve 18 with microchannel, and rotor 19 is embedded in sleeve 18, and with set
Cylinder 18 coordinates away from the end face of substrate 16, and rotor 19 is provided with the plug 21 for blocking and open any one micropore 20.
The power resources of rotor 19 are not restricted by, such as, can be provided by corollary equipment, it is also possible to for being separately provided
Micromachine on micro-fluidic chip or micromotor.
As shown in Figure 6, in mixing pit 2, gas circuit internal circulation system is mixing pit steam vent with the junction point of mixing pit 2
15, the sample ostium of mixing pit 2 is mixing pit inlet opening 14, and mixing pit inlet opening 14 is more than with the distance of center of rotation and mixes
Pond steam vent 15 and the distance of center of rotation.As shown in Figure 6, this can ensure also to be unlikely to enter after sample fills up mixing pit 2
Mixing pit steam vent 15, thus on the one hand the volume of mixing pit 2 is fully utilized, on the other hand also it is effectively ensured
In gas circuit internal circulation system, gas flowing is unimpeded.
Understandable, according to different samples, parallel connection, and the sample of parallel connection can be carried out by multiple sample cells 1
Pond 1 all connects with mixing pit 2, as shown in Figure 8, can realize multiple sample by this structure on a micro-fluidic chip
Process or realize different sample by the different sample cell 1 of control from the connection of mixing pit 2 order and mix with reagent successively；
Multiple mixing pits 2 can also be connected, depend on from different reagent as it is shown in fig. 7, the mixing pit 2 after Chuan Lian can realize same sample
Secondary realization mixes, reacts.
It is easily understood that the examination mutually of required reaction should be embedded with in sample cell 1, mixing pit 2 and reaction tank 3
Agent, these reagent are probably single, it is also possible to the mixing of many kinds of substance, and certainly, pre-buried reagent can be liquid or consolidate
State, powder, graininess, film like or gel etc., pre-buried mode can be to be directly added into liquid or solid matter, also
Solidification can be dried adding after liquid by naturally drying, dry, air-drying, by the way of lyophilizing.
Substrate 16 material of micro-fluidic chip can be the mixing of one or more in glass, silicon, metal, polymer
Thing, polymer can be PDMS (polydimethylsiloxane polydimethylsiloxane), PMMA (polymethyl
Methacrylate polymethyl methacrylate), PC engineering plastics, COC (copolymers of cycloolefin cycloolefin
Copolymer), PET (Polyethylene terephthalate polyethylene terephthalate), COP, ABS of Japanese auspicious father-in-law
In (Acrylonitrile butadiene Styrene copolymers acrylonitrile-butadiene-styrene copolymer) one
Planting or multiple, substrate and the cover plate coordinated with it of micro-fluidic chip use hot pressing, gluing, laser welder, ultra-sonic welded, screw thread
A kind of mode in tightening encapsulates.
Micro-fluidic chip disclosed in this utility model embodiment by simply, the most integrated, can complete biology,
The series of complex reaction in the field such as chemical, immune, it is possible to be automatically performed sample treatment in a chip, even detect
Overall process.
The using method of this integrated micro-flow control chip is as follows:
1) it is loaded in the sample cell of micro-fluidic chip by sample inlet；
2) sealed sample entrance and air vent；
3) under the auxiliary of corollary equipment, sample mixes with the reagent embedded in advance in sample cell and reacts, can
With rotary chip as required, or the temperature in sample cell is controlled；
4) low-speed centrifugal chip, by step 3) in liquid transfer in mixing pit, and under the auxiliary of corollary equipment, make
This liquid mixes with the reagent embedded in advance, reacts, this process rotary chip the most as required, or in sample cell
Temperature be controlled；
5) medium-speed centrifuge chip, by step 4) in mixed liquor transfer in distributing reservoir；
6) high speed centrifugation chip, is further transferred to liquid in distributing reservoir in each reaction tank；
7) under the auxiliary of corollary equipment, the reagent embedded in advance in making liquid and reaction tank reacts；
8) reaction result is detected and analyzed.
As can be seen here, integrated micro-flow control chip disclosed in the utility model, its architectural feature and corollary equipment are simple:
Centrifugal force is specifically utilized to drive liquid flowing in micro-fluidic chip；Utilize the plug control chip being positioned on the rotor of mixing pit
Fluid or the flowing of gas between interior different chamber；The gas circuit internal circulation system being interconnected is utilized to be uniformly controlled sample treatment
Air pressure balance required for the zoness of different such as district, mixed zone, reaction zone, it is to avoid the pollution problem in reaction tank, solves simultaneously
The sealing problem that mixing pit is with micro-fluidic chip integrated；The solid content utilizing sedimentation tank to have collected in reaction tank in solution, has
Effect avoids the step of purification needed for product；Utilize the relief area and sample cell, mixing pit, distribution being connected with sample cell
The special liquid barrier structure that the specific location connecting pipeline and gas circuit internal recycle pipeline between pond, reaction tank is arranged,
Effectively buffered due to liquid add overflow that thermal expansion causes and liquid transfer between differential responses pond during the overflow that causes.
This utility model full automatic treatment operation by fully integrated chip and corollary apparatus thereof, greatly simplifies staff's
Operation, improves work efficiency；One is provided for various ways reactions such as nucleic acid amplification reaction, biochemical reaction, immunoreation
Effective platform, can being widely used in clinical diagnosis, food safety, environmental monitoring etc., to need to use biological respinse many
Individual field.
Below as a example by the detection of saliva to integrated micro-flow control chip disclosed in this utility model make be used as
Further describe.
Saliva sample is joined by sample inlet in the sample cell of integrated micro-flow control chip, wrap in advance in sample cell
It is embedded with virolysis reagent；Said chip (abbreviation of integrated micro-flow control chip) is heated 30min by corollary equipment 65 DEG C,
Obtain viral nucleic acid extracting solution；Rotate mixing pit internal rotor, by the microporous connectivity sample being provided with in connecting pipeline and mixing pit
Pond and mixing pit, rotary chip is centrifuged 1min at 600 rpm, transfers to, in mixing pit, pass through by above-mentioned viral nucleic acid extracting solution
Rotate in the rotor in mixing pit makes mixing pit the constant-temperature amplification reagent embedded in advance to mix homogeneously with viral nucleic acid extracting solution；Rotation
Turning above-mentioned rotor connection mixing pit and distributing reservoir, rotary chip is centrifuged 1min at 800 rpm, is transferred to by above-mentioned mixed liquor point
Join in pond；Under 4000rpm, rotary chip is centrifuged 1min, by liquid uniform distribution in distributing reservoir to reaction tank, in reaction tank
It is embedded with in advance and plays the primer of specific reaction with sample nucleic acid；Chip is heated 60min, carries out constant temperature in reaction tank by 65 DEG C
Amplified reaction, finally uses necessary instrument detect the fluorescence in reaction tank in real time and draw testing result.
Above integrated micro-flow control chip provided by the utility model is described in detail.Tool used herein
Principle of the present utility model and embodiment are set forth by body example, and the explanation of above example is only intended to help to understand
Method of the present utility model and core concept thereof.It should be pointed out that, for those skilled in the art, do not taking off
On the premise of this utility model principle, it is also possible to this utility model is carried out some improvement and modification, these improve and modify
Also fall in this utility model scope of the claims.
Claims (22)
1. an integrated micro-flow control chip, its substrate (16) is provided with at least one integrated reaction unit, and described integrated
Reaction member at least includes sample cell (1), mixing pit (2) and reaction tank (3), the described sample cell connected by fluid passage (6)
(1) one end is provided with sample inlet (4), it is characterised in that also include gas circuit internal circulation system, described gas circuit internal circulation system
One end be connected with described mixing pit (2), the other end at least includes with described sample cell (1) away from described sample inlet (4)
The first circulation branch road that one end is connected.
Integrated micro-flow control chip the most according to claim 1, it is characterised in that also offer on described first circulation branch road
Have a steam vent to be opened/closed (54), and described gas circuit internal circulation system be additionally provided with near the position of described mixing pit (2) for
Block the second liquid blocking-up structure of liquid.
Integrated micro-flow control chip the most according to claim 1, it is characterised in that described integrated micro-flow control chip is tool
There are the centrifugal type microfludic chip of center of rotation, described sample cell (1), mixing pit (2) and described reaction tank (3) and described rotation
The distance at center increases successively.
Integrated micro-flow control chip the most according to claim 3, it is characterised in that also include being arranged at described reaction tank
(3) Buffer Pool (11) and between described mixing pit (2).
Integrated micro-flow control chip the most according to claim 3, it is characterised in that described reaction tank (3) includes multiple, institute
State reaction tank (3) the most equal with the distance of described center of rotation, and described mixing pit (2) passes through distribution with described reaction tank (3)
Passage (10) connects.
Integrated micro-flow control chip the most according to claim 5, it is characterised in that described distribution passage (10) is in wave
Shape, and its crest is near described center of rotation, trough is away from described center of rotation, described reaction tank (3) and described distribution passage
(10) wave trough position connection, described mixing pit (2) connects with the head end of described distribution passage (10).
Integrated micro-flow control chip the most according to claim 6, it is characterised in that another of described gas circuit internal circulation system
End also includes the second circulation branch road (9) connected with the end of described distribution passage (10).
Integrated micro-flow control chip the most according to claim 7, it is characterised in that described gas circuit internal circulation system is with described
The junction point of mixing pit (2) is referred to as the first junction point, the described first circulation branch road and described in described gas circuit internal circulation system
The junction point of the second circulation branch road is referred to as the second junction point, and the distance of described first junction point and described center of rotation is more than institute
State the distance of the second junction point and described center of rotation.
Integrated micro-flow control chip the most according to claim 7, it is characterised in that with the head end of described distribution passage (10)
A waste liquid pool (13) it is connected on the trough adjacent with end.
Integrated micro-flow control chip the most according to claim 7, it is characterised in that described second circulation branch road (9) is upper also
It is provided with first liquid blocking-up structure.
11. integrated micro-flow control chips according to claim 10, it is characterised in that described first liquid blocking-up structure is
First dilatation chamber (8) of volume discontinuities.
12. integrated micro-flow control chips according to claim 10, it is characterised in that described gas circuit internal circulation system is close
The position of described mixing pit (2) is additionally provided with the second liquid blocking-up structure for blocking liquid.
13. integrated micro-flow control chips according to claim 12, it is characterised in that described second liquid blocking-up structure is
Second dilatation chamber (7) of volume discontinuities.
14. integrated micro-flow control chips according to claim 12, it is characterised in that described first liquid blocking-up structure and
Described second liquid blocking-up structure uses hydrophobic material to make, or described first liquid blocking-up structure and described second liquid block
Hydrophobic layer it is coated with in structure.
15. integrated micro-flow control chips according to claim 5, it is characterised in that also include and described reaction tank (3) phase
Even, and with the distance of described center of rotation it is more than the sedimentation tank (12) of described reaction tank (3).
16. according to the integrated micro-flow control chip described in claim 1-15 any one, it is characterised in that described mixing pit
(2) including:
The sleeve (18) being arranged in described substrate (16) one side；
Run through the two sides of described substrate (16), and at least two micropore (20) that connect internal with described sleeve (18)；
It is embedded in described sleeve (18), and the rotor coordinated away from the end face of described substrate (16) with described sleeve (18)
(19), described rotor (19) is provided with the plug (21) for blocking and open any one of micropore (20).
17. according to the integrated micro-flow control chip described in claim 1-15 any one, it is characterised in that described sample cell
(1) include the multiple of parallel connection, and described sample cell (1) all connects with described mixing pit (2).
18. according to the integrated micro-flow control chip described in claim 1-15 any one, it is characterised in that described mixing pit
(2) including multiple, and described mixing pit (2) is serially connected.
19. integrated micro-flow control chips according to claim 7, it is characterised in that described gas circuit internal circulation system and institute
The junction point stating mixing pit (2) is mixing pit steam vent (15), and the sample ostium of described mixing pit (2) is mixing pit inlet opening
(14), described mixing pit inlet opening (14) and the distance of described center of rotation are more than described mixing pit steam vent (15) and described rotation
Turn the distance at center.
20. according to the integrated micro-flow control chip described in claim 1-15 any one, it is characterised in that also include one end with
Described first circulation branch road is connected, the relief area that the other end is connected with described sample cell (1).
21. integrated micro-flow control chips according to claim 1, it is characterised in that described sample cell (1), mixing pit (2)
It is embedded with required reaction reagent with in reaction tank (3).
22. integrated micro-flow control chips according to claim 1, it is characterised in that described substrate (16) and coordinating with it
Cover plate use a kind of mode in hot pressing, gluing, laser welder, ultra-sonic welded, screwed tight to encapsulate.
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| CN201620455263.6U CN205797240U (en) | 2016-05-18 | 2016-05-18 | A kind of integrated micro-flow control chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620455263.6U CN205797240U (en) | 2016-05-18 | 2016-05-18 | A kind of integrated micro-flow control chip |
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| CN205797240U true CN205797240U (en) | 2016-12-14 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240919 Address after: 102206 No. 18, life science Road, Beijing, Changping District Patentee after: CAPITALBIO Corp. Country or region after: China Patentee after: TSINGHUA University Address before: 102206 No. 18, life science Road, Beijing, Changping District Patentee before: CAPITALBIO Corp. Country or region before: China |