CN205740986U - A kind of multi-functional nucleic acid augmentative instrument - Google Patents
A kind of multi-functional nucleic acid augmentative instrument Download PDFInfo
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- CN205740986U CN205740986U CN201620284715.9U CN201620284715U CN205740986U CN 205740986 U CN205740986 U CN 205740986U CN 201620284715 U CN201620284715 U CN 201620284715U CN 205740986 U CN205740986 U CN 205740986U
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- orifice plates
- nucleic acid
- functional nucleic
- instrument
- acid augmentative
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Abstract
The utility model discloses a kind of multi-functional nucleic acid augmentative instrument, including control system, Switching Power Supply, host computer, heating circulation system and sample module, described control system connects described Switching Power Supply, host computer and heating circulation system, described heating circulation system be connected above described sample module, described sample module is provided with 12 orifice plates of 384 orifice plates of 0.2mL, 96 orifice plates, 48 orifice plates and 1.5mL.The multi-functional nucleic acid augmentative instrument that this utility model provides, can realize the heated constant temperature bath of 384 a large amount of samples of sample of centrifuge tube of 0.2mL, PCR cycle amplification, constant-temperature amplification；Also centrifuge tube and the constant temperature bath of centrifuge tube 12 orifice plate of 1.5mL, PCR cycle amplification, the constant-temperature amplification etc. of the 0.2mL in low-volume samples 48 hole can be realized.
Description
Technical field
This utility model relates to gene-amplificative instrament technical field, is specifically related to a kind of multi-functional nucleic acid augmentative instrument.
Background technology
PCR gene amplification instrument be a for providing simulation gene amplification time temperature device needed for each stage, and
Increase the function such as low temperature storage and fluoroscopic examination, it is adaptable to human genome project learns, prudence, oncology, tissue and colony
The polymerization in the clinical diagnosis fields such as the research fields such as biology, paleontology, zoology, botany and virus, tumor, heredopathia
Enzyme chain reaction fluorescent quantitation detects.
PCR instrument has the heating and cooling of program to sample, through temperature cycles many times to reach the purpose of amplification gene.
Each temperature cycles of its program generally has 3 temperature spots: first is denaturation temperature, usually 94-98 DEG C；Second is
Annealing temperature, sets as required by usually 50-65 DEG C；3rd is elongating temperature, usually 68-74 DEG C.React at PCR
During beginning, sample is heated to denaturation temperature by PCR instrument, and keeps several seconds.Then sample temperature is dropped to annealing temperature, and
Keep several seconds.Then sample being heated to elongating temperature, and keeps a period of time, this time is determined by concrete reaction condition.
Sample is heated to denaturation temperature the most again, starts next temperature cycles.All PCR gene amplification instrument, include and write
Each phase temperature controls the host platform of program, then carries out merit to the module mechanism with refrigeration heating chip by host platform
Rate exports, and makes the test tube groove surfaces temperature on module mechanism reach the temperature needed for gene amplification, will be equipped with needing amplification examination
The test tube of agent expands in inserting the test tube groove on module mechanism, then exciting and absorbing fluorescence data by photoelectric probe
It is analyzed, thus draws the result of various judgement.
Currently also there is isothermal amplification technology, it is only necessary to a constant stable amplification, and need single constant-temperature amplification instrument, and expand
The quantity increased only has 4 rows 8 even hole, is only capable of expanding 32 samples, and common PCR only can be suitably used for the 200 μ LPCR pipes in 96 holes, this
For the laboratory or inspection center of a large amount of augmentation detection samples, need multiple stage PCR instrument could realize its testing goal, and mesh
Before not yet have for the amplification of 1.5mL centrifuge tube instrument or can the instrument of constant temperature.
Summary of the invention
The purpose of this utility model is to provide a kind of multi-functional nucleic acid augmentative instrument, is applicable not only to the 0.2mL of 96 orifice plates
PCR pipe, it may also be used for the centrifuge tube of 1.5mL, applying electronic constant temperature bath, PCR amplification, constant-temperature amplification；Solve current nucleic acid to expand
Increase instrument and be simply possible to use in the amplification of 200 μ L PCR pipe, the problems such as service efficiency is low.
For achieving the above object, this utility model is by the following technical solutions:
A kind of multi-functional nucleic acid augmentative instrument, including control system, Switching Power Supply, host computer, heating circulation system and sample
Module, described control system connects described Switching Power Supply, host computer and heating circulation system, the top of described heating circulation system
Connecting described sample module, described sample module is provided with 12 holes of 384 orifice plates of 0.2mL, 96 orifice plates, 48 orifice plates and 1.5mL
Plate.
Multi-functional nucleic acid augmentative instrument described above, it is preferable that 384 orifice plates of described 0.2mL, 96 casement plates, 48 orifice plates and
12 orifice plates of 1.5mL are integrated setting.
Multi-functional nucleic acid augmentative instrument described above, it is preferable that 384 orifice plates of described 0.2mL, 96 orifice plates, 48 orifice plates and
12 orifice plates of 1.5mL are for being independently arranged, and movable property is changed.
Multi-functional nucleic acid augmentative instrument described above, it is preferable that 384 orifice plates of described 0.2mL, 96 orifice plates, 48 orifice plates and
12 orifice plates of 1.5mL are each correspondingly provided with independent cover plate.
Multi-functional nucleic acid augmentative instrument described above, it is preferable that described heating circulation system includes: resistance filament plate, quasiconductor
Refrigerator and radiator, described semiconductor cooler is located at above described resistance filament plate, and described radiator is located at described resistance
The lower section of filament plate.
Multi-functional nucleic acid augmentative instrument described above, it is preferable that the lower section of described radiator is provided with blower fan.
The multi-functional nucleic acid augmentative instrument that this utility model provides, can realize 384 a large amount of samples of sample of centrifuge tube of 0.2mL
Heated constant temperature bath, PCR cycle amplification, constant-temperature amplification；Also centrifuge tube and the 1.5mL of the 0.2mL in low-volume samples 48 hole can be realized
The heated constant temperature bath of centrifuge tube 12 orifice plate, PCR cycle amplification, constant-temperature amplification etc..
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model one preferred embodiment.
Fig. 2 is the Split type structure schematic diagram of this utility model one preferred embodiment.
Detailed description of the invention
It should be noted that in the case of not conflicting, the embodiment in the application and the feature in embodiment can phases
Combination mutually.Turn next to accompanying drawing and describe this utility model in conjunction with the embodiments in detail.
This utility model is to solve current nucleic acid augmentative instrument, and the little of 0.2mL being simply possible to use in 96 samples is centrifuged
The PCR amplification of pipe, uses the problems such as single, utilization rate is low, it is provided that an a large amount of sample of confession, and availability is high, and large sample amount adds
The multi-functional nucleic acid augmentative instrument that the amplification of heat, PCR cycle, constant-temperature amplification several functions are integrated in one.This multi-functional nucleic acid augmentative instrument
Including: control system, Switching Power Supply, host computer, heating circulation system and sample module, described control system connects described switch
Power supply, host computer and heating circulation system, described heating circulation system be connected above described sample module, described sample module
It is provided with 12 orifice plates of 384 orifice plates of 0.2mL, 96 orifice plates, 48 orifice plates and 1.5mL.
Its operation principle: under software, host computer sends control command and receives life to control system, control system
Order, controls power supply, the work of heating circulation system, makes the temperature of sample module meet the temperature of program setting of host computer.For
Can be used in fluoroscopic examination, control system controls light source and the work of data collecting card, the signal of collecting sample module, and send
Entering host computer, host computer receives the signal that data collecting card is sent here, and is analyzed it, and print data is reported, the most upper
Machine receives control system, the work state information that feedback is come, and the duty of real-time display instrument.
As it is shown in figure 1, multi-functional nucleic acid augmentative instrument, in housing, it is provided with heating system, control system and host computer, in heating
System be arranged over sample module, sample module is divided into four regions, be respectively equipped with 0.2mL 384 orifice plate 1,96 orifice plates 2,
12 orifice plates 4 of 48 orifice plates 3 and 1.5mL；Host computer also includes that display screen 5 and heating schedule set button 7, and Switching Power Supply 6 sets
Set the side of button 7 at heating schedule, certain Switching Power Supply is set in other sides of housing as required.Display screen 5 can
It is set to touching display screen it can also be used to set heating schedule, show the real-time status of each sample form.
Four regions of sample module, can control to use, as in figure 2 it is shown, corresponding A district is provided with 384 orifice plates of 0.2mL respectively
1, corresponding B district is provided with 96 orifice plates 2 of 0.2mL, and corresponding C district is provided with 48 orifice plates 3 of 0.2mL, and corresponding D district is provided with 12 holes of 1.5mL
Plate 4.Wherein, 384 orifice plates, 96 orifice plates, 48 orifice plates and 12 orifice plates can be wholely set, convenient installation.
As required, each orifice plate can be independently arranged, say, that 384 orifice plates, 96 orifice plates, 48 orifice plates and 12 holes
Plate each be independent plate, movable property is dismantled, and can realize freely changing required plate.384 orifice plates, 96 orifice plates are arranged
For module size plate of the same size, simply the arrangement in hole is different from number, the module size of 48 orifice plates and 12 orifice plates is set to
Plate of the same size, is only that size and the number in hole, spread configuration are different.
The setting of movable property dismounting, can be by being provided with prominent limit bottom the three of the edge of each orifice plate outsides
Edge, is provided with the groove that can engage with edge on the semiconductor cooler of heating circulation system, prominent edge is inserted this recessed
In groove, each orifice plate can be fitted tightly over the top of heating circulation system, it is ensured that temperature conduction accurate, and realize removable
Purpose.
As required, time to be used, need the amplification of a large amount of sample, 96 orifice plates can be disassembled, be mounted to 384 orifice plates,
The amplification of 768 samples or the requirement of water bath with thermostatic control can be realized, 48 orifice plates of 0.2mL can be replaced by 1.5mL centrifugal equally
12 orifice plates of pipe, can be used for the water-bath of the centrifuge tube of 24 1.5mL, it is simple to when lots of genes extracts, and reach wanting of water bath with thermostatic control
Ask.
In order to can guarantee that in PCR amplification procedure, keep the temperature on the centrifuge tube top of PCR, and ensureing invisible spectro examination
Agent is not vaporized, and is arranged over cover plate at sample form.Owing to regional is individually to be used separately, by corresponding for cover plate each
Individual region is separately provided, and corresponding four orifice plates arrange cover plate, relatively independent use, convenient use respectively, also do not interfere with each
The amplification of region samples or the effect of constant temperature.
In order to realize higher temperature-controlled precision and temperature homogeneity, use resistance wire and semiconductor cooler as temperature control unit
Part.Specifically, heating circulation system includes: resistance filament plate, semiconductor cooler and radiator, and described semiconductor cooler is located at
Above described resistance filament plate, described radiator is located at the lower section of described resistance filament plate.Semiconductor cooler can heat up, and again can
Cooling, simple in construction, more lightly facilitate.In order to make heat conduction more rapid, when assembling instrument, at resistance filament plate, partly lead
Heat-conducting silicone grease is scribbled between chiller and radiator and sample module contact face.
Heat in order to be able to produce after making heating quickly sheds, and is provided with fan in the lower section of radiator, and in casing pair
The position answered is provided with air vent or vent window.
Multi-functional nucleic acid augmentative instrument of the present utility model, is applicable not only to PCR amplification, could be applicable to constant-temperature amplification, also
Can be used for water bath with thermostatic control to use.Regional individually controls, and is used alone, and utilization rate is high, can effectively reduce cost.
The above, be only preferred embodiment of the present utility model, is not other form of doing this utility model
Restriction, any those skilled in the art are changed possibly also with the technology contents of the disclosure above or are modified as equivalent
The Equivalent embodiments of change.But every without departing from technical solutions of the utility model content, real according to technology of the present utility model
Any simple modification, equivalent variations and the remodeling that confrontation above example is made, still falls within the guarantor of technical solutions of the utility model
Protect scope.
Claims (6)
1. a multi-functional nucleic acid augmentative instrument, it is characterised in that include control system, Switching Power Supply, host computer, heat cycles system
System and sample module, described control system connects described Switching Power Supply, host computer and heating circulation system, described heat cycles system
System be connected above described sample module, described sample module be provided with 384 orifice plates of 0.2mL, 96 orifice plates, 48 orifice plates and
12 orifice plates of 1.5mL.
Multi-functional nucleic acid augmentative instrument the most as claimed in claim 1, it is characterised in that 384 orifice plates of described 0.2mL, 96 casements
12 orifice plates of plate, 48 orifice plates and 1.5mL are integrated setting.
Multi-functional nucleic acid augmentative instrument the most as claimed in claim 1, it is characterised in that 384 orifice plates of described 0.2mL, 96 orifice plates,
12 orifice plates of 48 orifice plates and 1.5mL are for being independently arranged, and movable property is changed.
Multi-functional nucleic acid augmentative instrument the most as claimed in claim 1, it is characterised in that 384 orifice plates of described 0.2mL, 96 orifice plates,
12 orifice plates of 48 orifice plates and 1.5mL are each correspondingly provided with independent cover plate.
Multi-functional nucleic acid augmentative instrument the most as claimed in claim 1, it is characterised in that described heating circulation system includes resistance wire
Plate, semiconductor cooler and radiator, described semiconductor cooler is located at above described resistance filament plate, and described radiator is located at
The lower section of described resistance filament plate.
Multi-functional nucleic acid augmentative instrument the most as claimed in claim 5, it is characterised in that the lower section of described radiator is provided with blower fan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620284715.9U CN205740986U (en) | 2016-04-07 | 2016-04-07 | A kind of multi-functional nucleic acid augmentative instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620284715.9U CN205740986U (en) | 2016-04-07 | 2016-04-07 | A kind of multi-functional nucleic acid augmentative instrument |
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| CN205740986U true CN205740986U (en) | 2016-11-30 |
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| CN201620284715.9U Expired - Fee Related CN205740986U (en) | 2016-04-07 | 2016-04-07 | A kind of multi-functional nucleic acid augmentative instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652288A (en) * | 2019-02-25 | 2019-04-19 | 成都瀚辰光翼科技有限责任公司 | A kind of automation Genotyping device and method |
| CN112945420A (en) * | 2021-02-02 | 2021-06-11 | 四川淯源仪器科技有限公司 | Temperature calibration system of wireless PCR instrument |
| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
-
2016
- 2016-04-07 CN CN201620284715.9U patent/CN205740986U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652288A (en) * | 2019-02-25 | 2019-04-19 | 成都瀚辰光翼科技有限责任公司 | A kind of automation Genotyping device and method |
| CN109652288B (en) * | 2019-02-25 | 2022-06-28 | 成都瀚辰光翼科技有限责任公司 | Automatic genotyping equipment and method |
| CN112945420A (en) * | 2021-02-02 | 2021-06-11 | 四川淯源仪器科技有限公司 | Temperature calibration system of wireless PCR instrument |
| CN112945420B (en) * | 2021-02-02 | 2024-05-10 | 四川淯源仪器科技有限公司 | Temperature calibration system of wireless PCR instrument |
| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161130 Termination date: 20190407 |
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| CF01 | Termination of patent right due to non-payment of annual fee |