CN201626959U - Micro perfusion device for cell culture - Google Patents
Micro perfusion device for cell culture Download PDFInfo
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- CN201626959U CN201626959U CN 201020106925 CN201020106925U CN201626959U CN 201626959 U CN201626959 U CN 201626959U CN 201020106925 CN201020106925 CN 201020106925 CN 201020106925 U CN201020106925 U CN 201020106925U CN 201626959 U CN201626959 U CN 201626959U
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- slight irrigation
- bottle
- cell
- flow reactor
- pipeline
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The utility model discloses a micro perfusion device for cell culture, which comprises a perfusion bottle and a waste liquid bottle, and also comprises a micro perfusion reactor arranged between the perfusion bottle and the waste liquid bottle, wherein one end of the micro perfusion reactor is provided with a liquid inlet, and the other end thereof is provided with a liquid outlet; the perfusion bottle is communicated with the liquid inlet of the micro perfusion reactor through a pipeline; the waste liquid bottle is communicated with the liquid outlet of the micro perfusion reactor through a pipeline; the micro perfusion reactor comprises a groove body capable of being sealed, and a cell culture hole plate pore plate arranged in the groove body; the cell culture pore plate comprises a flat plate and a plurality of culture holes arranged on the flat plate; and the bottom of the culture hole is provided with a semipermeable membrane the pore diameter of which can prevent cell to be cultured from passing through and but can make culture liquid pass through. With the utility model, the cell metabolic poison can be timely removed through the liquid flow, cell differentiation caused by the accumulation of cell metabolin is prevented and the pollution probability is effectively reduced.
Description
Technical field
The utility model relates to bioengineering field, particularly a kind of slight irrigation stream device that is used for cell cultures.
Background technology
Embryonic stem cell research is a big focus of current international biomedical sector.By reprogramming of somatic cells is the appearance of induced multi-potent stem cells technology, especially with stem-cell research field band to a new height.Induced multi-potent stem cells is closely similar at aspects such as cellular form, growth characteristics, surface marker, formation teratoma and mouse ES cells, so may be defined as embryonic stem cell-like.The embryonic stem cell of at present a lot of species is separated and is cultivated at subculture in vitro separately in body.Because it can and be induced to differentiate into any adult functive cell in external infinite copy, embryonic stem cell has wide application prospect in fields such as scientific research medical treatment such as genetically engineered and neomorph treatments.
At stem cell isolating initial stage or to induce the myeloid-lymphoid stem cell preinduction period be the critical period that stem cell adapts to external environment and reprogrammed in the animal body, the cell concentration in this period is few, and the mono-clonal after the screening needs single culture.Break up in the process of development to functioning cell stem cell, whenever take a step forward, stem cell is just lost some metabolic functions, the easier cell that is divided into other types of the metabolic stem cell of low speed, there are some researches show that the support of an all-round metabolic system is arranged in the stem cell tenuigenin.In traditional cell cultivation process, also cause lactic acid, ammonia and other metabolite to gather easily in the nutrient solution midium or long term, and the accumulation of other factor such as high osmotic pressure and reactive oxygen species, also may cell growth inhibiting, and cell is finally lost vigor and productivity.If can reduce the influence of these unfavorable factor cell growth as far as possible, under more efficiently metabolism state (when Δ L/ Δ G ratio is low), it is higher that cell concn might reach.In July, 2009, of being published on " science " magazine of people such as the Wang of department of biochemistry of Texas ,Usa university studies show that, unique hypermetabolism approach that mouse embryo stem cell exists---the Threonine cracking of threonate dehydrogenase mediation.This research has outstanding reference value to the metabolic basic theories of embryonic stem cell, has disclosed and should satisfy its special nutritional need and hypermetabolism condition in the stem cell culturing process.
The utility model content
The purpose of this utility model is to overcome above the deficiencies in the prior art, a kind of independent culture environment that can satisfy cell monoclonal is provided, can in time get rid of the cellular metabolism poisonous substance again, prevent the slight irrigation stream device that is used for cell cultures of the cytodifferentiation that the cell metabolite accumulation causes.
The slight irrigation stream device that is used for cell cultures that the utility model provides, comprise perfusion bottle and waste liquid bottle, also comprise the slight irrigation flow reactor that is arranged between perfusion bottle and the waste liquid bottle, one end of described slight irrigation flow reactor is provided with fluid inlet, the other end is provided with liquid outlet, described perfusion bottle is connected by the fluid inlet of pipeline with described slight irrigation flow reactor, and described waste liquid bottle is connected by the liquid outlet of pipeline with described slight irrigation flow reactor;
Described slight irrigation flow reactor comprises closed cell body and is arranged on the intravital cell cultures orifice plate of described groove, described cell cultures orifice plate comprises dull and stereotyped and opens a plurality of culture hole on described flat board, and the bottom of described culture hole is that the aperture can stop and treats that culturing cell passes but can realize the semi-permeable membranes that cell culture fluid passes through.
Be equipped with the gas filter that passes described bottle cap on the bottle cap of above-mentioned perfusion bottle and waste liquid bottle.Like this can be so that the pressure in jar stream bottle and the waste liquid bottle is with extraneous identical, and can completely cut off the bacterial contamination wherein of extraneous sky.
The bottle cap of above-mentioned perfusion bottle is provided with the pH meter that passes described bottle cap.The measuring junction of pH meter contacts with substratum in the perfusion bottle, can monitor the PH changing conditions of substratum in the perfusion bottle so in real time.
Above-mentioned pipeline is provided with at least one peristaltic pump.The flow velocity of control medium liquid in slight irrigation stream device is to adapt to the nutritional needs of cell like this, and the flow velocity of may command liquid is 0.02-0.45ml/h.
Specifically, on the pipeline between the fluid inlet of described perfusion bottle and slight irrigation flow reactor a peristaltic pump is set; A peristaltic pump is set on the liquid outlet of described slight irrigation flow reactor and the pipeline between the waste liquid bottle.
The fluid inlet of above-mentioned slight irrigation flow reactor and liquid outlet are angular distribution, can realize the maximization of fluid exchange area like this.
The fluid inlet of described slight irrigation flow reactor and be provided with the pipeline folder with the junction of described pipeline; The liquid outlet of described slight irrigation flow reactor and be provided with the pipeline folder with the junction of described pipeline can reinforce the connection like this.
The shape of above-mentioned culture hole can be U type or V-type.
Above-mentioned semi-permeable membranes is the wetting ability semi-permeable membranes.
Above-mentioned cell can be the positive cell mono-clonal of embryonic stem cell or transgenosis screening.
The hole wall of above-mentioned flat board and described culture hole is one-body molded, also can realize dismountable connection by inserting mode.
Above-mentioned semipolar linkage can be the Anopore film, and this Anopore film is a hydrophilic film, and the culture hole in the described cell cultures orifice plate can be 96 holes.
The utility model in use, nutrient solution places the perfusion bottle, motion by peristaltic pump is transported to fluid inlet with nutrient solution in input tube, enter in the slight irrigation flow reactor base of flowing through then, flow into by the Anopore film in the culture hole on top, for the cell in the culture hole provides nutrition, grow up for cell, can be seen through the Anopore film by the used medium liquid of cell, arrive Anopore film bottom, be transported in the waste liquid bottle by output tube and collect; In the control process of liquid medium rate of flow of fluid, the flow velocity of liquid can be adjusted the flow velocity of medium liquid at any time by the control of the peristaltic pump on input tube and the output tube according to the demand of cell cultures.So on the one hand fresh culture is constantly pumped into, the waste liquid that contains metabolism waste product (lactic acid, ammonia etc.) is on the other hand constantly discharged, by regulating irrigation rate, can make the mass transfer between nutritive substance and metabolism waste product and the cell, reduce harmful metabolic waste concentration accumulation.
The slight irrigation stream device that is used for cell cultures of the present utility model, not only can satisfy the independent culture environment of stem cell monoclonal, and can monitor the interior pH value of substratum in real time, in time get rid of the cellular metabolism poisonous substance by liquid-flow, better simulated in vivo environment, prevent the cytodifferentiation that the cell metabolite accumulation causes, promote stem cell clone and growth; It is few to have operation steps, can effectively reduce the characteristics of pollution probability.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment 1.
Fig. 2 is the vertical view of the slight irrigation flow reactor of the utility model embodiment 1.
Fig. 3 is the synoptic diagram of culture hole.
Figure number explanation: 1 perfusion bottle, 2 and 11 gas filters, 3pH meter, 4 input tubes, 5 and 9 peristaltic pumps, 6 and 8 pipelines folder, 7 slight irrigation flow reactors, 10 output tubes, 12 waste liquid bottles, 13 fluid inlets, 14 bases, 15 lids, 16 cell cultures orifice plates, 17 liquid outlets, 18Anopore film, 19 culture hole, 20 flat boards.
Embodiment
The utility model is described in further detail below in conjunction with specific embodiment, but the utility model is not limited to following examples.
As shown in Figure 1, a kind of slight irrigation stream device that is used for cell cultures comprises perfusion bottle 1 and waste liquid bottle 12.Be respectively equipped with on the bottle cap of the bottle cap of perfusion bottle 1 and waste liquid bottle 12 and pass the gas filter 2 and 11 of bottle cap separately, like this can be so that the pressure in jar stream bottle 1 and the waste liquid bottle 12 is with extraneous identical, and can completely cut off the bacterial contamination wherein of extraneous sky.Also be provided with the pH meter 3 of the bottle cap that passes the perfusion bottle on the perfusion bottle 1, the measuring junction of pH meter 3 contacts with substratum in the perfusion bottle, can monitor the PH changing conditions of substratum in the perfusion bottle so in real time.
As shown in Figure 1, slight irrigation stream device provided by the invention also comprises slight irrigation flow reactor 7, and this slight irrigation flow reactor 7 is arranged between perfusion bottle 1 and the waste liquid bottle 12.One end of described slight irrigation flow reactor 7 is provided with fluid inlet 13, the other end is provided with liquid outlet 17, wherein perfusion bottle 1 is connected with the fluid inlet 13 of slight irrigation flow reactor 7 by input tube 4, and waste liquid bottle 12 is connected with the liquid outlet 17 of described slight irrigation flow reactor 7 by output tube 10.In order to reinforce the connection, be provided with pipeline folder 6 in the junction of fluid inlet 13 and input tube 4, be provided with another pipeline folder 8 in the junction of liquid outlet 17 and output tube 10.As shown in Figure 2, for realizing the maximization of fluid exchange area, the fluid inlet 13 and the liquid outlet 17 of slight irrigation flow reactor 7 are angular distribution.
In order to control the flow velocity of medium liquid in slight irrigation stream device to adapt to the nutritional needs of cell, can on input tube 4 and output tube 10, peristaltic pump 5 and 9 be set respectively, the flow velocity of may command liquid is 0.02-0.45ml/h.
As illustrated in fig. 1 and 2, slight irrigation flow reactor 7 comprises closed cell body and is arranged on the intravital cell cultures orifice plate 16 of described groove.Wherein cell body comprises base 14 and lid 15, can realize sealing on the described base 14 when lid 15 places, and prevents to pollute.
As shown in Figure 3, cell cultures orifice plate 16 comprises flat board 20 and a plurality of culture hole 19 of opening on described flat board.The bottom of culture hole 19 is exactly a semi-permeable membranes 18, and semi-permeable membranes 18 is 2mm with the distance of cell body bottom (also being the bottom of base 14).
The pore size of semi-permeable membranes 18 with can stop treat culturing cell pass but the nutrient solution that can realize cell by being as the criterion.In the present embodiment, semi-permeable membranes 18 is Anopore films, and the aperture of Anopore film is 0.2 μ m.The shape of culture hole 19 can be U type or V-type, adopts the U type in the present embodiment.
When making cell cultures orifice plate 16, flat board 20 can be one-body molded with the hole wall of culture hole 19, also can realize dismountable connection by inserting mode, preferably adopt integrated mode to connect the hole wall of flat board 20 in the present embodiment with culture hole 19, be stained with the Anopore film in the bottom of each culture hole 19 then, constitute the bottom of culture hole 19.
The utility model in use, nutrient solution places perfusion bottle 1, motion by peristaltic pump 5 is transported to fluid inlet 13 with nutrient solution in input tube 4, enter in the slight irrigation flow reactor 7 base 14 of flowing through then, in the culture hole 19 by 18 inflows of Anopore film, for the cell in the culture hole 19 provides nutrition, grow up for cell, can be seen through Anopore film 18 by the used medium liquid of cell, arrive Anopore film 18 bottoms, be transported in the waste liquid bottle 12 by output tube 10 and collect; In the control process of liquid medium rate of flow of fluid, the flow velocity of liquid can be adjusted the flow velocity of medium liquid at any time by peristaltic pump 5 on input tube 4 and the output tube 10 and 9 controls according to the demand of cell cultures.So on the one hand fresh culture is constantly pumped into, the waste liquid that contains metabolism waste product (lactic acid, ammonia etc.) is on the other hand constantly discharged, by regulating irrigation rate, can make the mass transfer between nutritive substance and metabolism waste product and the cell, reduce harmful metabolic waste concentration accumulation.
The embodiment of the above is one of case study on implementation of the present utility model; be not to limit practical range of the present utility model with this; so the equivalence that all shapes according to the utility model, structure and principle are done changes, and all should be encompassed in the protection domain of the present utility model.
Claims (10)
1. the slight irrigation stream device that is used for cell cultures, comprise perfusion bottle and waste liquid bottle, it is characterized in that: described slight irrigation stream device also comprises the slight irrigation flow reactor that is arranged between perfusion bottle and the waste liquid bottle, one end of described slight irrigation flow reactor is provided with fluid inlet, the other end is provided with liquid outlet, described perfusion bottle is connected by the fluid inlet of pipeline with described slight irrigation flow reactor, and described waste liquid bottle is connected by the liquid outlet of pipeline with described slight irrigation flow reactor;
Described slight irrigation flow reactor comprises closed cell body and is arranged on the intravital cell cultures orifice plate of described groove, described cell cultures orifice plate comprises dull and stereotyped and opens a plurality of culture hole on described flat board, and the bottom of described culture hole is that the aperture can stop and treats that culturing cell passes but can realize the semi-permeable membranes that cell culture fluid passes through.
2. slight irrigation stream device according to claim 1 is characterized in that: be equipped with the gas filter that passes described bottle cap on the bottle cap of described perfusion bottle and waste liquid bottle.
3. slight irrigation stream device according to claim 2, it is characterized in that: the bottle cap of described perfusion bottle is provided with the pH meter that passes described bottle cap.
4. according to the arbitrary described slight irrigation stream device of claim 1-3, it is characterized in that: described pipeline is provided with at least one peristaltic pump.
5. slight irrigation stream device according to claim 4 is characterized in that: a peristaltic pump is set on the pipeline between the fluid inlet of described perfusion bottle and slight irrigation flow reactor; A peristaltic pump is set on the liquid outlet of described slight irrigation flow reactor and the pipeline between the waste liquid bottle.
6. according to the arbitrary described slight irrigation stream device of claim 1-3, it is characterized in that: the fluid inlet of described slight irrigation flow reactor and liquid outlet are angular distribution.
7. slight irrigation stream device according to claim 4, it is characterized in that: the fluid inlet of described slight irrigation flow reactor and liquid outlet are angular distribution.
8. slight irrigation stream device according to claim 5, it is characterized in that: the fluid inlet of described slight irrigation flow reactor and liquid outlet are angular distribution.
9. slight irrigation according to claim 6 stream device is characterized in that: the fluid inlet of described slight irrigation flow reactor and be provided with the pipeline folder with the junction of described pipeline; The liquid outlet of described slight irrigation flow reactor and be provided with the pipeline folder with the junction of described pipeline.
10. slight irrigation stream device according to claim 6, it is characterized in that: the shape of described culture hole is U type or V-type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020106925 CN201626959U (en) | 2010-02-01 | 2010-02-01 | Micro perfusion device for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020106925 CN201626959U (en) | 2010-02-01 | 2010-02-01 | Micro perfusion device for cell culture |
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| CN201626959U true CN201626959U (en) | 2010-11-10 |
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| CN 201020106925 Expired - Lifetime CN201626959U (en) | 2010-02-01 | 2010-02-01 | Micro perfusion device for cell culture |
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Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329726A (en) * | 2011-09-01 | 2012-01-25 | 山西医科大学 | Cell culture dish |
| CN102952753A (en) * | 2011-08-29 | 2013-03-06 | 北京清美联创干细胞科技有限公司 | Automatic perfusion device for preparing induced pluripotent stem cells |
| CN103087917A (en) * | 2011-10-27 | 2013-05-08 | 北京清美联创干细胞科技有限公司 | Apparatus used for preparing olfactory ensheathing cells |
| CN103087913A (en) * | 2011-11-07 | 2013-05-08 | 北京清美联创干细胞科技有限公司 | Automatic perfusion device for preparing CIK cells |
| WO2014179196A1 (en) * | 2013-04-30 | 2014-11-06 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
| CN104845881A (en) * | 2014-02-17 | 2015-08-19 | 上海泰因生物技术有限公司 | Apparatus and method for producing anti-CD52 monoclonal antibodies through online industrially robust regulation of cell states |
| CN106318866A (en) * | 2015-06-24 | 2017-01-11 | 中国科学院大连化学物理研究所 | Small multi-purpose cell culturing and testing device |
| CN107384789A (en) * | 2017-07-27 | 2017-11-24 | 九三极恒生物医药科技江苏有限公司 | For culture medium apparatus for automatic change in nanogel 3D cell cultivation process |
| CN108148749A (en) * | 2016-12-05 | 2018-06-12 | 中国科学院大连化学物理研究所 | Keep the micro-fluidic chip culture systems of culture solution dissolved oxygen concentration and acid-base value |
| CN109055210A (en) * | 2018-07-05 | 2018-12-21 | 曾小敏 | The device of inexpensive cell culture |
| CN112567016A (en) * | 2018-08-20 | 2021-03-26 | 爱平世股份有限公司 | Cell culture device |
| US11345880B2 (en) | 2017-07-14 | 2022-05-31 | Corning Incorporated | 3D cell culture vessels for manual or automatic media exchange |
| CN115595311A (en) * | 2022-12-15 | 2023-01-13 | 巴德生物科技有限公司（Cn） | Method and kit for rapidly screening monoclonal cells |
| US11584906B2 (en) | 2017-07-14 | 2023-02-21 | Corning Incorporated | Cell culture vessel for 3D culture and methods of culturing 3D cells |
| US11613722B2 (en) | 2014-10-29 | 2023-03-28 | Corning Incorporated | Perfusion bioreactor platform |
| US11661574B2 (en) | 2018-07-13 | 2023-05-30 | Corning Incorporated | Fluidic devices including microplates with interconnected wells |
| US11732227B2 (en) | 2018-07-13 | 2023-08-22 | Corning Incorporated | Cell culture vessels with stabilizer devices |
| US11767499B2 (en) | 2017-07-14 | 2023-09-26 | Corning Incorporated | Cell culture vessel |
| US11857970B2 (en) | 2017-07-14 | 2024-01-02 | Corning Incorporated | Cell culture vessel |
| US11912968B2 (en) | 2018-07-13 | 2024-02-27 | Corning Incorporated | Microcavity dishes with sidewall including liquid medium delivery surface |
| US11976263B2 (en) | 2014-10-29 | 2024-05-07 | Corning Incorporated | Cell culture insert |
| US12146154B2 (en) | 2020-08-28 | 2024-11-19 | Corning Incorporated | Spheroid cell culture article and methods thereof |
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2010
- 2010-02-01 CN CN 201020106925 patent/CN201626959U/en not_active Expired - Lifetime
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102952753A (en) * | 2011-08-29 | 2013-03-06 | 北京清美联创干细胞科技有限公司 | Automatic perfusion device for preparing induced pluripotent stem cells |
| CN102329726A (en) * | 2011-09-01 | 2012-01-25 | 山西医科大学 | Cell culture dish |
| CN102329726B (en) * | 2011-09-01 | 2016-03-02 | 山西医科大学 | Tissue Culture Dish |
| CN103087917A (en) * | 2011-10-27 | 2013-05-08 | 北京清美联创干细胞科技有限公司 | Apparatus used for preparing olfactory ensheathing cells |
| CN103087913A (en) * | 2011-11-07 | 2013-05-08 | 北京清美联创干细胞科技有限公司 | Automatic perfusion device for preparing CIK cells |
| WO2014179196A1 (en) * | 2013-04-30 | 2014-11-06 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
| US9790465B2 (en) | 2013-04-30 | 2017-10-17 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
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| CN104845881A (en) * | 2014-02-17 | 2015-08-19 | 上海泰因生物技术有限公司 | Apparatus and method for producing anti-CD52 monoclonal antibodies through online industrially robust regulation of cell states |
| US11976263B2 (en) | 2014-10-29 | 2024-05-07 | Corning Incorporated | Cell culture insert |
| US11613722B2 (en) | 2014-10-29 | 2023-03-28 | Corning Incorporated | Perfusion bioreactor platform |
| CN106318866A (en) * | 2015-06-24 | 2017-01-11 | 中国科学院大连化学物理研究所 | Small multi-purpose cell culturing and testing device |
| CN106318866B (en) * | 2015-06-24 | 2019-06-18 | 中国科学院大连化学物理研究所 | A kind of multipurpose small cell culture and experimental provision |
| CN108148749A (en) * | 2016-12-05 | 2018-06-12 | 中国科学院大连化学物理研究所 | Keep the micro-fluidic chip culture systems of culture solution dissolved oxygen concentration and acid-base value |
| US11345880B2 (en) | 2017-07-14 | 2022-05-31 | Corning Incorporated | 3D cell culture vessels for manual or automatic media exchange |
| US11970682B2 (en) | 2017-07-14 | 2024-04-30 | Corning Incorporated | 3D cell culture vessels for manual or automatic media exchange |
| US11584906B2 (en) | 2017-07-14 | 2023-02-21 | Corning Incorporated | Cell culture vessel for 3D culture and methods of culturing 3D cells |
| US11767499B2 (en) | 2017-07-14 | 2023-09-26 | Corning Incorporated | Cell culture vessel |
| US11857970B2 (en) | 2017-07-14 | 2024-01-02 | Corning Incorporated | Cell culture vessel |
| CN107384789A (en) * | 2017-07-27 | 2017-11-24 | 九三极恒生物医药科技江苏有限公司 | For culture medium apparatus for automatic change in nanogel 3D cell cultivation process |
| CN109055210A (en) * | 2018-07-05 | 2018-12-21 | 曾小敏 | The device of inexpensive cell culture |
| US11732227B2 (en) | 2018-07-13 | 2023-08-22 | Corning Incorporated | Cell culture vessels with stabilizer devices |
| US11661574B2 (en) | 2018-07-13 | 2023-05-30 | Corning Incorporated | Fluidic devices including microplates with interconnected wells |
| US11912968B2 (en) | 2018-07-13 | 2024-02-27 | Corning Incorporated | Microcavity dishes with sidewall including liquid medium delivery surface |
| CN112567016A (en) * | 2018-08-20 | 2021-03-26 | 爱平世股份有限公司 | Cell culture device |
| US12146154B2 (en) | 2020-08-28 | 2024-11-19 | Corning Incorporated | Spheroid cell culture article and methods thereof |
| CN115595311A (en) * | 2022-12-15 | 2023-01-13 | 巴德生物科技有限公司（Cn） | Method and kit for rapidly screening monoclonal cells |
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