CN1679700A - Medicinal preparation containing notoginseng and pueraria root for treating cardio-cerebral blood vessel diseases and its preparing method - Google Patents
Medicinal preparation containing notoginseng and pueraria root for treating cardio-cerebral blood vessel diseases and its preparing method Download PDFInfo
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Abstract
A Chinese medicine for treating cerebrovascular and cardiovascular diseases is prepared from notoginseng and pueraria root through extracting.
Description
Technical field:
The present invention relates to a kind of pharmaceutical preparation for the treatment of cardiovascular and cerebrovascular disease and preparation method thereof, said preparation is formed through extracting processing and preparing by Chinese medicine Radix Notoginseng, Radix Puerariae.
Background technology:
Cardiovascular and cerebrovascular disease as coronary heart disease, cerebral infarction, is human disease and main causes of death, accounts for 1/3rd of general mortality rate, has surpassed cancer at its mortality rate of China.And along with the raising of China along with living standards of the people, the crowd who suffers from cardiovascular disease is huge day by day, leave invalid among the survivor, cause huge life to threaten to the patient, the patient needs to take medicine for a long time or all the life more simultaneously, quality of life is had a strong impact on, and causes white elephant also for patient family and even entire society.
At present, the Chinese medicine and western medicine of treatment coronary heart disease is a lot, but how many Western medicine has some side effect, and is not almost having effective method aspect the treatment of cerebrovascular disease.Chinese medicine has certain characteristic aspect the treatment cardiovascular and cerebrovascular disease, utilizes activating blood circulation to dissipate blood stasis method treatment cardiovascular and cerebrovascular disease that certain effect has been arranged, and becomes the focus of cardiovascular and cerebrovascular disease area research in recent years.
The invention provides a kind of Chinese prescription for the treatment of cardiovascular and cerebrovascular disease and preparation method thereof, form, extract pharmaceutically active substance, press pharmaceutical dosage form and add adjuvant, make preparation with the galenic pharmacy routine techniques by Radix Notoginseng and Radix Puerariae.Radix Notoginseng has removing stasis to stop bleeding, the function of the analgesic therapy of invigorating blood circulation.Cardiovascular and cerebrovascular disease mostly is deficiency in origin and excess in superficiality, the card of blood stasis due to qi deficiency.Therefore, if emphasize blood circulation promoting and blood stasis dispelling in the treatment simply, then can injure the human righteousness, it is empty to increase the weight of losing of healthy energy, and clinical can the appearance such as the curative effect instability, electrocardiogram improvement rate is lower, takes for a long time and causes nervous phenomenon such as breathe hard.And the pseudo-ginseng blood-circulation-invigovating blood stasis dispelling has the characteristics of " blood stasis dispelling is not just hindered ", simultaneously the strong effect of tonify deficiency is arranged again, can take into account the human righteousness in blood circulation promoting and blood stasis dispelling, so very suitable the use.Modern pharmacological research proves, Radix Notoginseng has tangible blood vessel dilating effect, can coronary artery dilator, cerebrovascular and peripheral blood vessel, increase coronary flow and cerebral blood flow, improve cerebral circulation, can also reduce myocardial oxygen consumption, reduce myocardial contraction, strengthen the hypoxia-bearing capability of heart and brain tissues, obviously to resisting myocardial ischemia and cerebral ischemia-reperfusion injury; Radix Notoginseng also has effects such as blood fat reducing, anti peroxidation of lipid, removing oxygen-derived free radicals, anticoagulant and thrombosis.The active component of Radix Notoginseng is that Radix Notoginseng total arasaponins is (referring to Yang Yun, Zhang Jing, the Chen Yuting chief editor, Natural Medicine Chemistry component extraction separation handbook (revised edition), Beijing: China Traditional Chinese Medicine Publishing House, 2003, second edition, 17 pages), the main component of the XUESHUANTONG ZHUSHEYE of list marketing, XUESAITONG ZHUSHEYE is exactly a Radix Notoginseng total arasaponins, clinical to be used for the treatment of cardiovascular and cerebrovascular disease respond well, no obvious adverse reaction.Drug use Radix Puerariae of the present invention and Radix Notoginseng compatibility use.Radix Puerariae has the function of promoting blood circulation and stopping pain, its main active be puerarin (referring to Pu Zihe, Wang Ning, the pharmaceutical research of Radix Puerariae progress, northwest pharmaceutical journal, 2000,15 (2): 81-83).Puerarin can be expanded the coronary vasodilator of normal and spasm significantly, thereby improves the myocardial blood flow of ischemic region, and this is to realize by its direct relexation to vascular smooth muscle.Puerarin can also obviously reduce the generation of the myocardium lactic acid that causes because of ischemia, reduces oxygen consumption and creatine phosphokinase burst size, and ultrastructure myocardium behind the ischemia-reperfusion is also made moderate progress, and shows that puerarin can improve myocardial metabolism.Puerarin can make the speed of heartbeat slow down, and myocardial contraction strengthens, and aortic pressure reduces, but does not reduce ischemic region CC blood flow, and this and puerarin are that a kind of beta receptor antagonist has substantial connection; After the dog hat was pricked, intramuscular injection gave puerarin, can promote myocardium side to prop up the open and formation of circulation, and ischemic myocardium is had protective effect.The effect that puerarin is also useful to cerebral circulation, can promote normal Golden Hamster cerebral circulation and improve the local microcirculation obstacle that modeling causes, mainly show as the amplitude that increases the blood capillary motion, improve regional blood flow, and now existing puerarin injection listing is strengthened in dosage increase effect.Drug use Radix Notoginseng of the present invention and Radix Puerariae compatibility compare with the above-mentioned preparation that has gone on the market, have increased active constituents of medicine; In addition, the mechanism of action of Radix Notoginseng total arasaponins, puerarin is also inequality, and Radix Notoginseng total arasaponins to the protective effect of cardiac-cerebral ischemia hypoxic damage is and its blocking-up Ca
2+The effect of passage is closely related, puerarin then may be mainly by its directly lax vascular smooth muscle, retardance beta receptor, to promote that myocardium side is propped up circulation open and form, improve approach such as myocardial metabolism and work.Therefore, both compatibilities use has increased action target spot and approach, has strengthened curative effect.
Summary of the invention:
The invention provides a kind of pharmaceutical preparation, said preparation is prepared from Chinese medicine Radix Notoginseng, Radix Puerariae, said preparation contains the pseudo-ginseng activity composition of effective dose and the Radix Puerariae active component of effective dose, the pseudo-ginseng activity composition is made with Radix Notoginseng, the Radix Puerariae active component is made with Radix Puerariae, in the preparation of per 1000 units, the amount that the pseudo-ginseng activity composition that contains is amounted to into crude drug is 2000-8000 part, and the amount that the Radix Puerariae active component that contains is amounted to into crude drug is 2000-8000 part.
Preferably Radix Notoginseng 3000-5000 part, Radix Puerariae 3000-5000 part.
More preferably 4000 parts of Radix Notoginseng, 4000 parts of Radix Puerariaes.
Described pseudo-ginseng activity composition is selected from: Radix Notoginseng total arasaponins, ginsenoside Rg
1, ginsenoside Rb
1, ginsenoside Re, Panax Notoginseng saponin R
1, Panax Notoginseng saponin R
2, dencichine, the Radix Puerariae active component is selected from: Radix Puerariae total flavones, Radix Puerariae isoflavone, puerarin, daiazi, daidzein.
Above pseudo-ginseng activity composition and Radix Puerariae active component can extract with the method for routine and make, it can be crude extract, also can extract, extract preferably, described crude extract, generally without purification step, described extract generally passes through purification step, and pseudo-ginseng activity composition and Radix Puerariae active component also can be bought from the market and obtain.
In more than forming, the weight of medicine is calculated with crude drug, and per 1 part can be 1 gram, also can be kilogram or ton, if be unit with gram, this prescription composition can be made into 1000 doses of pharmaceutical preparatioies.Described 1000 doses of fingers, the final drug preparation of making, as make 1000 of capsule preparations, 1000 in tablet, granule 1000g, oral liquid 1000ml etc., also can make big packing as granule, as the 100-500 bag, specifically can be 100 bags, 125 bags, 200 bags, 250 bags, 500 bags etc., every bag can be used as taking dose 1 time.
More than form, can be made into the preparation of 50-1000 taking dose, as tablet, make 1000, each taking dose can be the 1-20 sheet, can take 50-1000 time altogether.As granule, make 125 bags, take the 1-2 bag at every turn, can take 62.5-125 time altogether.
More than form to be by weight as proportioning, when producing, can increase or reduce according to corresponding proportion, as large-scale production can be unit with the kilogram, or be unit with the ton, small-scale production can be unit with the milligram also, weight can increase or reduce, but the constant rate of the raw medicinal herbs weight proportion between each composition.
The ratio of above weight proportion obtains through science screening, for especial patient, and as serious symptom or light disease, fat or modest patient, the proportioning of the amount of can corresponding adjustment forming increases or reduces being no more than 100%, and drug effect is constant.
Pharmaceutical preparation of the present invention is to process through extraction or other modes by the raw material of Chinese medicine that above-mentioned prescription is formed, and makes pharmaceutically active substance, subsequently, with this material is raw material, adds the medicine acceptable carrier when needing, and makes according to the routine techniques of galenic pharmacy.Described active substance can obtain by extracting raw material of Chinese medicine respectively, also can obtain by the co-extracted raw material of Chinese medicine, also can obtain by other modes, as: by pulverize, squeeze, calcine, grind, sieve, percolation, extraction, water are carried, alcohol extraction, ester are carried, methods such as ketone is carried, chromatography obtain, these active substances can be the material of extractum form, can be that dry extract also can be a fluid extract, make different concentration according to the different needs decision of preparation.
Pharmaceutically active substance in the pharmaceutical preparation of the present invention, its shared percentage by weight in preparation can be 0.1-99.9%, all the other are the medicine acceptable carrier.Pharmaceutical preparation of the present invention exists with unit dosage form, and described unit dosage form is meant the unit of preparation, as every of tablet, capsular every capsules, every of injection etc., in the unit dose, the amount that contains active substance is 5-800mg, preferably 20-500mg.
Pharmaceutical preparation of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, capsule, oral liquid, syrup, granule, pill, powder, unguentum, sublimed preparation, injection, suppository, cream, spray, drop pill, patch, slow releasing preparation, controlled release preparation.
Pharmaceutical preparation of the present invention, the preparation of its oral administration can contain excipient commonly used, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet in case of necessity.
The filler that is suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant, for example magnesium stearate.The acceptable wetting agent of appropriate drug comprises sodium lauryl sulphate.
Can fill by mixing, the method that tabletting etc. are commonly used prepares solid oral composition.Mix repeatedly active substance is distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid for example can be aqueous or oily suspensions, solution, Emulsion, syrup or elixir, perhaps can be a kind of available water before use or other suitable composite dry products of carrier.This liquid preparation can contain conventional additive, such as suspending agent, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat, emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if desired, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this chemical compound can be suspended or dissolving.The preparation of solution is normally by being dissolved in active substance in a kind of carrier filter-sterilized before it is packed into a kind of suitable bottle or ampoule, sealing then.For example a kind of local anesthetic of adjuvant, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after the bottle of packing into that this compositions is freezing, and under vacuum, water is removed.
Pharmaceutical preparation of the present invention, when being prepared into medicament, optionally add suitable medicine acceptable carrier, described medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, the EDTA disodium, EDTA calcium sodium, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, the phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Pharmaceutical preparation of the present invention, active component can extract two flavor medicines respectively, and extraction also can mix.When extracting respectively, method is as follows:
The preparation of Radix Puerariae active component: get Radix Puerariae and add 5～9 times of amount 65～75% alcohol reflux secondaries respectively, the time is 1～3 hour at every turn, has extracted the back and has reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, and reuse 40～80% ethanol carry out desorbing, collect eluent, cross polyamide column after having reclaimed ethanol, and gradient elution is collected the eluents that different sections are divided, and recrystallization got puerarin after eluent was concentrated;
The preparation of Radix Notoginseng total arasaponins: get and add 5～9 times of amount 65～75% alcohol reflux secondaries after pseudo-ginseng is pulverized respectively, the time is 1～3 hour at every turn, has extracted the back and has reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, and reuse 40～80% ethanol carry out desorbing, collect eluent, have reclaimed the ethanol after drying and have got dry extract, and dried cream adds 85%～95% ethanol extraction and gets Radix Notoginseng total arasaponins;
More than two kinds of active component mix, as active constituents of medicine, add the medicine acceptable carrier after, make pharmaceutical preparation by the galenic pharmacy routine techniques.
Medicine of the present invention has coronary artery dilator, cerebrovascular and peripheral blood vessel, increase coronary flow and cerebral blood flow, improve cerebral circulation, can also reduce myocardial oxygen consumption, obviously, also have anti peroxidation of lipid, effects such as removing oxygen-derived free radicals, anticoagulant and thrombosis to resisting myocardial ischemia and the effect of cerebral ischemia-reperfusion injury.
Below beneficial effect by pharmacological evaluation explanation medicine of the present invention:
(every 1000ml, by Radix Notoginseng 4000g, Radix Puerariae 4000g makes for Radix Notoginseng, Radix Puerariae compound injection.Hereinafter to be referred as compound injection) pharmacological research
One, to the protective effect of myocardial ischemia
Get the Wistar rat, be divided into 4 groups at random, be i.e. blank group, model group, compound injection group, XUESHUANTONG ZHUSHEYE matched group.Model is irritated stomach according to group give distilled water 4ml/kg, all the other each groups are pressed the table 1 dosage corresponding medicinal liquid of lumbar injection respectively.Be administered once every day, continuous 6 days.Behind the last administration 1h, urethane (5%, face upward the position and fix by 20ml/kg) anesthetized rat, cut off animal skin along midsternal line, at left border of sternum 6～7 intercostal openings, extrude heart rapidly after, ligation at once left side heart coronary artery anterior descending branch root, heart is put back to the thoracic cavity, close thoracic cavity and stitching, take out heart behind the 6h ,-20 ℃ of preservations are spent the night.In the experimentation, respectively at (normally), ligation before the operation at once, 2h, 6h recording ecg after the ligation, observe that the ST section changes and myocardial ischemia improvement situation.Active and malonaldehyde (MDA) content of superoxide dismutase (SOD) is measured in the muscular tissue homogenate of coring.
Table 1 compound injection is to the influence of ST section change absolute value and myocardial ischemia scope
| Group | ????n | Dosage mg/kg | ST section change absolute value (x ± s) | Myocardial ischemia scope ratio | ||
| Ligation at once | ????2h | ????6h | ||||
| Model group compound injection group XUESHUANTONG group | ????12 ????14 ????13 | ????- ????100 ????70 | ????2.44±1.30 ????2.48±1.27 ????2.44±1.44 | ????2.34±1.36 ????1.30±1.24 **△????????1.76±1.31 * | ????2.57±1.19 ????1.16±1.20 **△????????1.56±1.24 * | ????0.32±0.08 ????0.16±0.07 **△????????0.21±0.05 * |
Annotate: compare with model group,
*P＜0.05,
*P＜0.01.
Compare with the XUESHUANTONG model group,
△P＜0.05.
As seen from the above table, compound injection is raised ECG ST section and is significantly improved behind the ligation 2h, and compound injection, XUESHUANTONG group are also significantly improved its variation during 6h, and the compound recipe group is better than XUESHUANTONG treatment group (P＜0.05) in the improvement of every index.And compound injection also is better than XUESHUANTONG group (P＜0.05) aspect the myocardial ischemia scope alleviating.
Table 2 compound injection is to the influence of rats with myocardial ischemia lipid peroxidation
| Group | ?n | Dosage (mg/kg) | SOD (U/g weight in wet base) | MDA (nmol/g weight in wet base) |
| Blank group model group compound injection group XUESHUANTONG group | ?15 ?12 ?14 ?13 | ??- ??- ??100 ??70 | 486.46±13.48 **340.24±13.51 469.77±16.44 **△432.14±16.38 ** | 130.4±15.6 **354.7±13.4 166.2±15.1 **△△228.7±18.6 ** |
Annotate: compare with model group,
*P＜0.01,
Compare with the XUESHUANTONG group,
△P＜0.05,
△ △P＜0.01.
As seen from the above table, compound injection can obviously improve rat heart muscle tissue SOD activity, reduces the level of myocardium MDA, and the prompting compound injection has the rat lipid peroxidation that resists myocardial ischemia to a certain degree, and its effect is better than the XUESHUANTONG ZHUSHEYE group.
In sum, compound injection has protective effect to the Acute Myocardial Ischemia in Rats that crown moving 9 arteries and veins of ligation are caused.
Two, to the protective effect of rat cerebral ischemia
(Wu Junfang, Shi Yiju, Liu Tianpei, berberine be to the protective effect of mice and rat cerebral ischemia, Chinese J Pharmacol Toxicol, 1995,9 (2): 100-103 for list of references.) described reversibility middle cerebral artery infraction (MCAO) method duplicates the focal cerebral ischemia in rats model.Behind the modeling 2h to the nervous symptoms of animal according to document (Bedexson JB.Pitts LH, Tsuji M, et al.Stroke, 1986,17:472.) mark, broken end is got brain after 24 hours, measures cerebral infarction scope, cerebral tissue superoxide dismutase (SOD) activity and malonaldehyde (MDA) content.
Table 3 compound injection is to the protective effect of rat cerebral ischemia
| Group | ?n | Dosage mg/kg | The nervous symptoms scoring | Cerebral infarction scope (%) | SOD (U/mgpr) | MDA (nmol/mgpr)) |
| Sham operated rats model group compound injection group XUESHUANTONG group | ?20 ?14 ?18 ?17 | ??- ??- ??100 ??70 | 0.6±1.1 **8.1±0.7 5.8±0.6 **△7.5±0.7 ** | 0 **13.1±1.8 8.0±1.3 **△10.2±1.6 * | 44.7±6.7 **20.4±7.6 36.6±5.1 **△28.7±5.3 * | 6.8±1.6 **22.1±2.6 12.8±3.7 **△16.4±3.2 * |
Annotate: compare with model group,
*P＜0.05,
*P＜0.01.
Compare with the XUESHUANTONG matched group,
△P＜0.05.
As seen from the above table, compound injection can obviously reduce the delayed ischemic neurological deficits grade scoring of rat model, obviously reduces the cerebral infarction scope, improves cerebral tissue SOD vigor, reduce MDA content (P all＜0.01), and its effect is better than XUESHUANTONG ZHUSHEYE (P＜0.05).Show that compound injection of the present invention has significant protective effect to MCAO cerebral ischemic model rat.
Acute toxicity testing and long term toxicity test prove that medicine of the present invention does not have obvious toxic and side effects, and hepatic and renal function, routine blood test, the blood parameters of animal do not had obvious influence.
A kind of injection for the treatment of cardiovascular and cerebrovascular disease of the present invention can be an injection, capsule, granule, tablet, optimizing injection.Composition Radix Notoginseng total arasaponins, puerarin can be mixed in suitable carriers, in diluent or the excipient.
The specific embodiment:
Specific embodiment is as follows, includes but not limited to the following example.
Embodiment 1
The preparation of injection
The preparation method of active component is as follows:
The preparation of Radix Puerariae active component: take by weighing Radix Puerariae 4000g, add 8 times of amounts, 6 times of amount 70% alcohol reflux secondaries respectively, the time was respectively 2 hours, 2 hours at every turn, had extracted the back and had reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 60% ethanol carries out desorbing, collect eluent, has reclaimed mistake polyamide column behind the ethanol, wash remove impurity with water, reuse 20% ethanol remove impurity, use 90% ethanol elution at last, collect eluent, eluent concentrates the back and gets puerarin 26.2g with re-crystallizing in ethyl acetate;
The preparation of Radix Notoginseng total arasaponins: get and add 7 times of amounts, 5 times of amount 70% alcohol reflux secondaries after pseudo-ginseng 4000g pulverizes respectively, the time was respectively 2 hours, 2 hours, had extracted back recovery ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 70% ethanol carries out desorbing, collects eluent, reclaimed ethanol after drying under reduced pressure get dry extract, dried cream adds 90% ethanol extraction and gets Radix Notoginseng total arasaponins 21.6g;
Get pseudo-ginseng activity composition and Radix Puerariae active component and add water for injection and mannitol, heated and stirred makes dissolving, transfers pH value, filters, add active carbon heating 15 minutes, cooling filters, and adds the volume that the injection water is settled to regulation, with the 0.2 μ m microporous filter membrane fine straining of sterilizing, degerming, bottling, lyophilization gets 1000 bottles (200mg/ bottles).
Embodiment 2
Capsule preparation method thereof,
The preparation method of active component is as follows:
The preparation of Radix Puerariae active component: take by weighing Radix Puerariae 4000g, add 8 times of amounts, 6 times of amount 70% alcohol reflux secondaries respectively, the time was respectively 2 hours, 2 hours at every turn, had extracted the back and had reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 60% ethanol carries out desorbing, collect eluent, has reclaimed mistake polyamide column behind the ethanol, wash remove impurity with water, reuse 20% ethanol remove impurity, use 90% ethanol elution at last, collect eluent, eluent concentrates the back and gets puerarin 27.1g with re-crystallizing in ethyl acetate;
The preparation of Radix Notoginseng total arasaponins: get and add 7 times of amounts, 5 times of amount 70% alcohol reflux secondaries after pseudo-ginseng 4000g pulverizes respectively, the time was respectively 2 hours, 2 hours, had extracted back recovery ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 70% ethanol carries out desorbing, collects eluent, reclaimed ethanol after drying under reduced pressure get dry extract, dried cream adds 90% ethanol extraction and gets Radix Notoginseng total arasaponins 21.9g;
Get that two kinds of active component of Radix Notoginseng and Radix Puerariae are pulverized, behind the mix homogeneously, add a certain amount of adjuvant mix homogeneously and incapsulate, make 1000 capsules, reinstall in the bottle after aluminum-plastic packaged and seal.
Embodiment 3
Method for preparing tablet thereof,
The preparation method of active component is as follows:
The preparation of Radix Puerariae active component: take by weighing Radix Puerariae 2000g, add 8 times of amounts, 6 times of amount 70% alcohol reflux secondaries respectively, the time was respectively 2 hours, 2 hours at every turn, had extracted the back and had reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 60% ethanol carries out desorbing, collect eluent, has reclaimed mistake polyamide column behind the ethanol, wash remove impurity with water, reuse 20% ethanol remove impurity, use 90% ethanol elution at last, collect eluent, eluent concentrates the back and gets puerarin 13g with re-crystallizing in ethyl acetate;
The preparation of Radix Notoginseng total arasaponins: get and add 7 times of amounts, 5 times of amount 70% alcohol reflux secondaries after pseudo-ginseng 8000g pulverizes respectively, the time was respectively 2 hours, 2 hours, had extracted back recovery ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 70% ethanol carries out desorbing, collects eluent, reclaimed ethanol after drying under reduced pressure get dry extract, dried cream adds 90% ethanol extraction and gets Radix Notoginseng total arasaponins 22g;
After getting two kinds of active component pulverizing of Radix Notoginseng and Radix Puerariae, mix homogeneously, add a certain amount of adjuvant mix homogeneously tabletting, make 1000.
Embodiment 4
Process for producing granula,
The preparation method of active component is as follows:
The preparation of Radix Puerariae active component: take by weighing Radix Puerariae 8000g, add 8 times of amounts, 6 times of amount 70% alcohol reflux secondaries respectively, the time was respectively 2 hours, 2 hours at every turn, had extracted the back and had reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 60% ethanol carries out desorbing, collect eluent, has reclaimed mistake polyamide column behind the ethanol, wash remove impurity with water, reuse 20% ethanol remove impurity, use 90% ethanol elution at last, collect eluent, eluent concentrates the back and gets puerarin 53g with re-crystallizing in ethyl acetate;
The preparation of Radix Notoginseng total arasaponins: get and add 7 times of amounts, 5 times of amount 70% alcohol reflux secondaries after pseudo-ginseng 2000g pulverizes respectively, the time was respectively 2 hours, 2 hours, had extracted back recovery ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, reuse 70% ethanol carries out desorbing, collects eluent, reclaimed ethanol after drying under reduced pressure get dry extract, dried cream adds 90% ethanol extraction and gets Radix Notoginseng total arasaponins 11g;
After getting two kinds of active component pulverizing of Radix Notoginseng and Radix Puerariae, mix homogeneously, add a certain amount of adjuvant mix homogeneously system granule, get the 1000g granule.
Claims (8)
1. pharmaceutical preparation for the treatment of cardiovascular and cerebrovascular disease, it is characterized in that, contain the pseudo-ginseng activity composition of effective dose and the Radix Puerariae active component of effective dose, the pseudo-ginseng activity composition is made with Radix Notoginseng, the Radix Puerariae active component is made with Radix Puerariae, in the preparation of per 1000 units, the amount that the pseudo-ginseng activity composition that contains is amounted to into crude drug is 2000-8000 part, and the amount that the Radix Puerariae active component that contains is amounted to into crude drug is 2000-8000 part.
2. the pharmaceutical preparation of claim 1 is characterized in that, in the preparation of per 1000 units, the amount that the pseudo-ginseng activity composition that contains is amounted to into crude drug is 3000-5000 part, and the amount that the Radix Puerariae active component that contains is amounted to into crude drug is 3000-5000 part.
3. the pharmaceutical preparation of claim 1 is characterized in that, in the preparation of per 1000 units, the amount that the pseudo-ginseng activity composition that contains is amounted to into crude drug is 4000 parts, and the amount that the Radix Puerariae active component that contains is amounted to into crude drug is 4000 parts.
4. any one pharmaceutical preparation of claim 1-3 is characterized in that described pseudo-ginseng activity composition is selected from: Radix Notoginseng total arasaponins, ginsenoside Rg
1, ginsenoside Rb
1, ginsenoside Re, Panax Notoginseng saponin R
1, Panax Notoginseng saponin R
2, dencichine, the Radix Puerariae active component is selected from: Radix Puerariae total flavones, Radix Puerariae isoflavone, puerarin, daiazi, daidzein.
5. profit requires 1 pharmaceutical preparation, wherein also comprises the medicine acceptable carrier.
6. the pharmaceutical preparation of claim 4 is injection, tablet, granule, capsule, drop pill or micropill.
7. the application of the pharmaceutical preparation of claim 1 in the medicine of preparation treatment cardiovascular and cerebrovascular disease.
8. the preparation method of the pharmaceutical preparation of claim 1 is characterized in that, the process following steps:
The preparation of Radix Puerariae active component: get Radix Puerariae and add 5～9 times of amount 65～75% alcohol reflux secondaries respectively, the time is 1～3 hour at every turn, has extracted the back and has reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, and reuse 40～80% ethanol carry out desorbing, collect eluent, cross polyamide column after having reclaimed ethanol, and gradient elution is collected the eluents that different sections are divided, and recrystallization got puerarin after eluent was concentrated;
The preparation of Radix Notoginseng total arasaponins: get and add 5～9 times of amount 65～75% alcohol reflux secondaries after pseudo-ginseng is pulverized respectively, the time is 1～3 hour at every turn, has extracted the back and has reclaimed ethanol, add water carry out water precipitating after centrifugal D
101Macroporous resin, the resin absorption post washes remove impurity with water, and reuse 40～80% ethanol carry out desorbing, collect eluent, have reclaimed the ethanol after drying and have got dry extract, and dried cream adds 85%～95% ethanol extraction and gets Radix Notoginseng total arasaponins;
More than two kinds of active component lump together, as active constituents of medicine, this active component with make preparation after the medication medication acceptable carrier mixes.
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| CN 200510004927 CN1679700A (en) | 2005-01-28 | 2005-01-28 | Medicinal preparation containing notoginseng and pueraria root for treating cardio-cerebral blood vessel diseases and its preparing method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185669B (en) * | 2006-11-23 | 2011-01-12 | 河北智同医药控股集团有限公司 | Medicine composition for treating cardiovascular and cerebrovascular diseases |
| CN108640957A (en) * | 2018-06-28 | 2018-10-12 | 湖南华诚生物资源股份有限公司 | A method of extracting a variety of pueraria isoflavone glycosides simultaneously from pueraria crude extract |
| CN110122718A (en) * | 2019-06-10 | 2019-08-16 | 重庆柠柠柒饮料有限公司 | The beverage of the gentle physical fatigue of strengthen immunity |
-
2005
- 2005-01-28 CN CN 200510004927 patent/CN1679700A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185669B (en) * | 2006-11-23 | 2011-01-12 | 河北智同医药控股集团有限公司 | Medicine composition for treating cardiovascular and cerebrovascular diseases |
| CN108640957A (en) * | 2018-06-28 | 2018-10-12 | 湖南华诚生物资源股份有限公司 | A method of extracting a variety of pueraria isoflavone glycosides simultaneously from pueraria crude extract |
| CN108640957B (en) * | 2018-06-28 | 2021-07-06 | 湖南华诚生物资源股份有限公司 | Method for simultaneously extracting various pueraria isoflavone glycosides from pueraria crude extract |
| CN110122718A (en) * | 2019-06-10 | 2019-08-16 | 重庆柠柠柒饮料有限公司 | The beverage of the gentle physical fatigue of strengthen immunity |
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