CN1659282A - Bacillus licheniformis mutant host cell - Google Patents
Bacillus licheniformis mutant host cell Download PDFInfo
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- CN1659282A CN1659282A CN038133164A CN03813316A CN1659282A CN 1659282 A CN1659282 A CN 1659282A CN 038133164 A CN038133164 A CN 038133164A CN 03813316 A CN03813316 A CN 03813316A CN 1659282 A CN1659282 A CN 1659282A
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Abstract
A Bacillus licheniformis mutant host cell comprising a mutation (deletion) in one or more genes encoding polypeptides having proteolytic activity wherein the mutant host cell expresses at least 5% less of the one or more proteases than the parent host cell, when cultivated under comparable conditions. The mutant host cell is used for producing heterologous polypeptides.
Description
Technical field
Genus bacillus (Bacillus sp.) is the interested host of preparation heterologous protein because they can be directly with protein excretion in substratum.They have the ability of high secretory protein, and are (amenable) that highly is obedient in heredity, and non-pathogenic (nonpathogenic), and do not have intracellular toxin.So, genus bacillus promptly Bacillus licheniformis (Bacillus licheniformis, B.licheniformis) in the efficient production justacrine from the multiple protein of different organisms (organisms).
In the biotechnology industry of high competition, even the genus bacillus host cell of improvement (improved) also needs a little, this host cell can provide, for example from product stability, say on the product purity etc., more interesting production system, or even be optional production system.
Background technology
The Industrial products that trade benefit can much be arranged with biological mode production in the genus bacillus host cell, heterologous polypeptide for example, amino acid, carbohydrate etc.Yet, also produce with genus bacillus type of production host cell homologous (native to) contaminative (contaminant) polypeptide with described product, some of them can be secreted into described substratum, and some of them can be retained in the cell or maintenance links to each other with cytolemma.This pollutant must be removed (removed) usually or suppress, for example guarantees, and the stability of described product or shelf life (shelf-life), or be used for obtaining enough product purities.
In Bacillus licheniformis, prepare heterologous polypeptide, especially can have the contaminative natural polypeptides of proteolytic activity and influenced owing to existence.Adopt different modes to avoid the problem of extracellular degraded, comprised the genetically deficient (Sloma etc., 1989 that make coding known protein enzyme; Wu etc., 1991; Sloma etc., 1991) or add protease inhibitor (Simonen﹠amp during the fermentation; Pala, 1993).
Summary of the invention
The problem to be solved in the present invention is, how a kind of Bacillus licheniformis host cell of improvement is provided, and the natural polypeptides that one or more of this cell expressing has proteolytic activity reduces.The invention provides a kind of way of head it off, this method be tested and appraised several from Bacillus licheniformis, have proteolytic activity, still unknown polypeptide up to now, and polynucleotides encoding them, make up a mutant host cell, these polypeptide of one or more of its expression and/or its homologue that is closely related reduce.
Therefore, one aspect of the present invention relates to, Bacillus licheniformis mutant host cell derived from parental generation Bacillus licheniformis host cell, described mutant host cell is encoded at one or more, and one or more has on the gene of polypeptide of proteolytic activity sudden change is arranged, described polypeptide and SEQ ID NO:2 to 64 (comprising SEQ ID NO:2 and 64), or SEQ ID NO:2 is identical to one or more polypeptide at least 80% shown in the SEQ ID NO:84 (comprising SEQ IDNO:2 and 84), preferably at least 85% is identical, more preferably at least 90% is identical, also more preferably at least 95% identical, most preferably at least 97% is identical, and the polypeptide that one or more of wherein said mutant host cell expression has proteolytic activity lacks at least 5% than the parental generation host cell of cultivating down at condition of equivalent (comparable conditions).One or more of preferred described mutant host cell expression has the polypeptide of proteolytic activity, lack at least 10% than the parental generation host cell of under condition of equivalent, cultivating, more preferably lack at least 20%, also more preferably lack at least 30%, also more preferably lack at least 40%, also more preferably lack at least 50%, or few at least 60%, or lack at least 70%, or lack at least 80%, or most preferably lack at least 90%.Most preferably, described mutant host cell is expressed the polypeptide that one or more has proteolytic activity anything but.
Must use condition of equivalent to cultivate, come in mutant host cell more of the present invention and the parental generation host cell, described one or more has proteolytic activity polypeptide expression level.Cultivate them respectively with identical setting (setup) under condition of equivalent, for example the conventional criteria of temperature control etc. is poor certainly to allow relevant with growth experiment usually operating parameters.Described one or more polypeptide expression level quantitatively be experimental technique with standard textbook known in the art, for example mRNA is quantitatively or based on (immuno-based) experiment of immunity.
The present invention relates on the other hand, a kind of method that in Bacillus licheniformis mutant host cell, prepares at least a interested product, it is included in cultivates the Bacillus licheniformis mutant host cell that above-mentioned aspect limits in the suitable medium, produce described product in this substratum.
The present invention relates in one aspect at last, the Bacillus licheniformis mutant host cell that first aspect limited, purposes at least a interested product of preparation, it is included in cultivates described mutant host cell in the suitable medium, produce described product in this substratum.
Definition
Nucleic acid construct (construct): term used herein " nucleic acid construct " refers to strand or double-stranded nucleic acid molecule, and it separates from naturally occurring gene or by modification (modified) and comprises nucleic acid fragment (segment) in a kind of natural non-existent mode.When described nucleic acid construct comprises when expressing the required control sequence of encoding sequence of the present invention this term nucleic acid construct and term " expression cassette (expression cassette) " synonym.
Control sequence: the term that this paper limited " control sequence " comprises that to express polypeptide of the present invention necessary or to its favourable all components.Each control sequence can with the nucleotide sequence homology or the allos of coding said polypeptide.This control sequence includes but not limited to, leader sequence, polyadenylation sequence (polyadenylation sequence), propeptide sequence, promotor, signal peptide sequence, and transcription terminator.Described control sequence comprises promotor at least, and transcribes and the translation termination signal.Described control sequence can belt lacing, to introduce the specific limited restriction enzyme site, impels described control sequence to be connected with the coding region of the nucleotide sequence of coded polypeptide.
Be operably connected: this paper limits term and " is operably connected " to a kind of configuration (configuration), and wherein control sequence is positioned at the appropriate location relevant with the encoding sequence of described dna sequence dna, thereby described control sequence can instruct polypeptide expression.
Encoding sequence: term used herein " encoding sequence " comprises nucleotide sequence, and it directly shows the aminoacid sequence of its protein product.The border of described encoding sequence is determined by open reading frame that usually it is usually from the ATG initiator codon.Described encoding sequence generally includes DNA, the nucleotide sequence of cDNA and reorganization.
Express: term in this paper context " expression " comprises any step that polypeptide production relates to, and it includes but not limited to transcribe, post transcriptional modificaiton, translation, posttranslational modification and secretion.
Expression vector: term in this paper context " expression vector " comprises linearity or Circular DNA molecular structure, and it comprises the fragment of code book invention polypeptide, and this fragment can be operatively attached to and makes on its other fragment of transcribing.
Summary of the invention
A kind of Bacillus licheniformis mutant host cell, it is derived from parental generation Bacillus licheniformis host cell, wherein said mutant host cell has one or more gene to undergo mutation, described genes encoding one or more have proteolytic activity and with SEQ ID NO:2 to SEQ ID NO:64 (comprising SEQ IDNO:2 and 64), or SEQ ID NO:2 is to the identical polypeptide of one or more polypeptide shown in the SEQ ID NO:84 (comprising SEQ ID NO:2 and 84) at least 80%, described one or more of wherein said mutant host cell expression has the polypeptide of proteolytic activity, lacks at least 5% than the parental generation host cell of cultivating under condition of equivalent.
Term in this paper context " parental generation host cell " refers to, one or more has the mutator gene of polypeptide of proteolytic activity except encoding at one or more of described mutant, in heredity (genetically) is identical with filial generation of the present invention (progeny) mutant or mutant cells, or the cell of isogenic (isogenic).
The identity of peptide sequence, or identity (%) can suitably be studied by carrying out sequence alignment with computer program known in the art, " GAP " (the Program Manual forthe Wisconsin Package that provides as the GCG routine package, Version 8, August 1994, Genetics Computer Group, 575Science Drive, Madison, Wisconsin, USA 53711) (Needleman, S.B.and Wunsch, C.D., (1970), Journal of Molecular Biology, 48,443-453).Use following the setting with GAP to carry out the dna sequence dna contrast: the point penalty that GAP produces is 5.0, and the point penalty that GAP extends is 0.3.
Purpose of the present invention provides a kind of bacterial cultures that does not contain natural bacteria proteolytic enzyme, and purifying products is dropped to minimum, and obtains better products stability and high product output.According to the present invention, that or those gene that this can have the natural polypeptides of proteolytic activity by mutagenesis coding reduces or even eliminates (abolish) described one or more expression of gene fully and finish.Guaranteeing that as everyone knows gene do not express one of method that becomes intracellular active polypeptide, in simple terms, is to make described encoding gene disappearance or excalation.This area has been described much about how from cellular genome (comprising certainly from the genome of Bacillus licheniformis cell) and has specifically been lacked or the technology (seeing for example Novozymes A/S WO 01/90393, Novozymes A/S WO 02/00907) of one or more gene of excalation.Therefore, the preferred embodiment of the invention relates to the host cell of first aspect, and it suddenlys change by part or all of disappearance one or more one or more gene with polypeptide of proteolytic activity of encoding.
The preferred embodiment of the invention relates to the host cell of first aspect, and two or more have on two or more genes of polypeptide of proteolytic activity and undergo mutation at coding for it.
By the interested product that the mutant host cell of first aspect produces, can be one or more polypeptide of one or more heterologous gene coding.So the preferred embodiment of the invention relates to the host cell of first aspect, it comprises one or more heterologous gene of one or more heterologous polypeptide of encoding.
In the industrial production of polypeptide, need to obtain high as far as possible product output.A kind of mode that increases output is the copy number that increases the gene of coding polypeptide of interest.This can be by placing the plasmid of high copy number to realize described gene.Yet plasmid is unsettled, and if in the culturing process of host cell non-selectivity pressure, it is often lost from host cell.The another kind of method that increases the copy number of described gene of interest is the karyomit(e) that it is incorporated into described host cell with the multiple copied number.WO 91/09129 and WO 94/14968 (Novozymes A/S) have described the integration of two genes, and its content is hereby incorporated by.The preferred embodiment of the invention relates to the host cell of first aspect, and wherein said heterologous gene exists at least 2 copies, preferably at least 4 copies, most preferably at least 6 copies.In another embodiment, there are at least ten copies in described heterologous gene.If carried by plasmid, can there be hundreds of copies of described gene in each cell, so in another embodiment of the present invention, there are at least 100 copies in described heterologous gene.
WO 99/41358 (Novozymes A/S) (its content is hereby incorporated by) has described two and has integrated with the compact arranged gene of antiparallel placed in-line mode, obtains stability preferably; And WO 02/00907 (Novozymes A/S) (its content is hereby incorporated by) has described the chromosomal multi-copy integration of gene.The preferred embodiment of the invention relates to the host cell of first aspect, and wherein said heterologous gene stable integration is to the genome of described cell.
Can be by using as the selected marker thing of antibiotic resistance markers selective staining body intasome easily.Yet, it is desirable to avoid using antibiotic resistance markers.WO 01/90393 disclose a kind of with gene integration in the karyomit(e) of host cell, and antibiotic resistance markers is not stayed method in the bacterial strain, its content is hereby incorporated by.The preferred embodiment of the invention relates to the host cell of first aspect, and wherein said heterologous gene is integrated in the karyomit(e) of described cell, and any antibiotic resistance markers gene is not stayed integration site.
The present invention also relates to nucleic acid construct, it comprises the nucleotide sequence of the product interested of encoding, this nucleotide sequence can be operably connected with one or more control sequence, this control sequence instructs described encoding sequence to express in the host cell that is fit under the condition compatible with described control sequence.
Can handle the nucleotide sequence of (manipulate) coding polypeptide of interest in many ways, express described polypeptide.Dirigibility before described nucleotide sequence is inserted carrier is ideal or necessity, and this depends on expression vector.Technology with the recombinant DNA method modified nucleotide sequence is known in this field.
Increasing other method of described product output (yield), is the promoter activity that improves the specific promotor of the expression of regulating specific gene of interest.Simultaneously, the activity that more fully strengthens simultaneously several promotors can make product output increase.Described control sequence can be suitable promoter sequence, is promptly discerned one section nucleotide sequence of expressing described nucleotide sequence by host cell.Described promoter sequence comprises transcriptional control sequence, and it mediates described polypeptide expression.Described promotor can be any nucleotide sequence that shows transcriptional activity in selected host cell, it comprises mutant, promotor brachymemma and heterozygosis, and can be outside Codocyte or in the cell polypeptide, obtain with described host cell homology or allogenic gene.
Instruct the example of the suitable promotor that nucleic acid construct of the present invention (particularly in bacterial host cell) transcribes, for deriving from following promotor: intestinal bacteria lac operon, the gelase gene (dagA) of streptomyces coelicolor (Streptomyces coelicolor), levansucrase (levansucrase) gene (sacB) of subtilis (Bacillussubtilis), the alpha-amylase gene of Bacillus licheniformis (amyL), maltose (maltogenic) amylase gene (amyM) of bacstearothermophilus (Bacillus stearothermophilus), the alpha-amylase gene (amyQ) of bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the penicillinase gene of Bacillus licheniformis (penP), xylA of subtilis and xylB gene, with procaryotic β-Nei Xiananmei gene (Villa-Kamaroff etc., 1978, Proceedings of the National Academy of Sciences USA 75:3727-3731), and tac promotor (DeBoer etc., 1983, Proceedings of the National Academy ofSciences USA80:21-25).More multiple promoter is seen " Useful proteins from recombinantbacteria " in Scientific American, 1980,242:74-94; With Sambrook etc., 1989 (seeing above) are described.
Other available promotor is seen WO93/10249, and WO 98/07846 and WO 99/43835 (Novozymes A/S) are described, and its full content is hereby incorporated by.The preferred embodiment of the invention relates to the host cell of first aspect, and wherein said heterologous gene is transcribed from allogeneic promoter or manual activation.
Described control sequence also can be suitable Transcription Termination subsequence, promptly a kind of sequence that stops transcribing of discerning for host cell.Described terminator sequence is operably connected to 3 ' end of the nucleotide sequence of coding said polypeptide.Any terminator sequence that works in selected host cell all can be used for the present invention.
Described control sequence also can be suitable leader sequence, the non-translational region of mRNA, and it is important for being translated by described host cell.Described leader sequence is operably connected to 5 ' end of the nucleotide sequence of coding said polypeptide.Any leader sequence that works in selected host cell all can be used for the present invention.
Described control sequence also can be the polyadenylation sequence, promptly is connected in to a kind of operability the sequence of 3 of described nucleotide sequence ' end, and it is added to polyadenous glycosides residue on the mRNA that transcribes as the signal of described host cell identification when transcribing.Any polyadenylation sequence that works at selected host cell all can be used for the present invention.
Described control sequence also can be signal peptide coding region, and the aminoacid sequence that its coding is connected with the N-terminal of polypeptide and guides the polypeptide that is encoded to enter described emiocytosis approach.5 ' end of the encoding sequence of described nucleotide sequence can congenital (inherently) comprise signal peptide coding region, and natural linking to each other of coding region fragment with the described secrete polypeptide of coding read in the frame in translation in the coding region of this signal peptide.Perhaps, 5 ' end of described encoding sequence can comprise and the allogenic signal peptide coding region of described encoding sequence.When described encoding sequence does not comprise signal peptide coding region natively, may need allogenic signal peptide coding region.Perhaps, available described allogenic signal peptide coding region substitute simply natural signals peptide-coding region, the secretion that improves described polypeptide.Yet any signal peptide coding region that instructs polypeptide expressed to enter the Secretory Pathway of selected host cell all can be used for the present invention.
The useful signal peptide-coding region of bacterial host cell is the signal peptide coding region that derives from the gene of following material: the maltogenic amylase of genus bacillus NCIB 11837, the α-Dian Fenmei of bacstearothermophilus, Bacillus licheniformis subtilisin (subtilisin), the β-Nei Xiananmei of Bacillus licheniformis, neutral protease (the nprT of bacstearothermophilus, nprS, nprM) and subtilis prsA.More signal peptides are seen Simonen and Palva, 1993, and Microbiological Reviews 57:109-137 is described.
Described control sequence also can be the preceding peptide-coding region that coding is positioned at the aminoterminal aminoacid sequence of polypeptide.The gained polypeptide is called proenzyme (proenzyme) or propolypeptide (propolypeptide) (or under the certain situation proenzyme (zymogen)).Propolypeptide is non-activity normally, and can be converted into sophisticated active polypeptide by cutting described propetide from propolypeptide catalysis or autocatalytically.Peptide-coding region can derive from the gene of following material before described: the Sumizyme MP of subtilis (aprE), the neutral protease of subtilis (nprT), α-the factor of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), the aspartate protease of Rhizomucor miehei (Rhizomucor miehei) and the thermophilic laccase (laccase) (WO 95/33836) of ruining an enzyme (Myceliophthorathermophila).
When signal peptide and propetide district were present in the N-terminal of polypeptide, described propetide district was positioned at by the N-terminal of described polypeptide, and described signal peptide district is positioned at by the N-terminal in described propetide district.
It is desirable to, add the regulating and controlling sequence of the regulation and control polypeptide expression relevant with described host cell growth.The example of described regulator control system is, answers chemistry or physical stimulation (comprise and have regulating compound), opens or closes those regulator control systems of (turned on or off) described expression of gene.Regulator control system in the prokaryotic organism system comprises: lac, tac and trp operon system.In the yeast, available ADH2 system or GAL1 system.In the eukaryote system, regulator control system comprises dihydrofolate reductase gene (it increases) and metallothionein gene (they increase with heavy metal) when Rheumatrex exists.In the case, the nucleotide sequence of coding said polypeptide can be operably connected with regulating and controlling sequence.
The present invention also relates to comprise the recombinant expression vector of nucleic acid construct of the present invention.Above-mentioned multiple Nucleotide and control sequence can interconnect (joined together) and produce recombinant expression vector, and this carrier comprises one or more restriction site easily, the feasible nucleotide sequence that can insert or substitute coding said polypeptide in described site.Perhaps, can express nucleotide sequence of the present invention by described nucleotide sequence or the nucleic acid construct that comprises described sequence are inserted suitable expression vector.When creating described expression vector, described encoding sequence is arranged in carrier, thereby makes encoding sequence be operably connected to suitable expression control sequenc.
Described recombinant expression vector can be any carrier (for example, plasmid or virus), and it can be handled with recombinant DNA method easily, and can express described nucleotide sequence.Select carrier to depend on the consistency of the host cell that described carrier and described carrier are introduced into usually.Described carrier can be linear or the closed hoop plasmid.
Described carrier can be an autonomously replicationg vector, i.e. the carrier that exists as the outer entity of karyomit(e), and it duplicates and does not rely on THE REPLICATION OF CHROMOSOME, for example, plasmid, extra-chromosomal element, minichromosome, or the duplicating of artificial chromosome.
Described carrier can comprise any instrument (means) of guaranteeing self-replication.Perhaps, described carrier can be when introducing described host cell, is be integrated into genome and duplicates with the karyomit(e) that it was integrated into.In addition, available single carrier or plasmid perhaps comprise the two or more carriers or the plasmid of the genomic all DNA that will introduce described host cell or available transposon altogether.
Carrier of the present invention preferably comprises one or more selected marker thing, and it can make selects cell transformed to become easy.The selected marker thing is a kind of gene, and its product provides biocide (biocide) or virus resistance, heavy metal resistance, prototroph of auxotroph (prototrophy to auxotroph) or the like.
The selected marker thing example of bacterium is dal gene of subtilis or the dal gene of Bacillus licheniformis, or brings antibiotics resistance, as penbritin, and kantlex, the marker of paraxin or tetracyclin resistance.
Carrier of the present invention preferably comprises a kind of element, and it makes described carrier stable integration to the genome of described host cell, or makes described carrier not rely on genome in cell and independently duplicate.
For being integrated into described host cell gene group, described carrier can be dependent on the nucleotide sequence of coding said polypeptide, or makes described carrier stably be incorporated into genomic any other carrier element by homology or non-homogeneous reorganization.Perhaps, described carrier can comprise additional nucleotide sequence, instructs the genome that is incorporated into described host cell by homologous recombination.This additional nucleotide sequence makes described carrier be integrated into the exact position of the group of host cell gene described in the karyomit(e).Be incorporated into the possibility in accurate site for raising, described integrated element should preferably comprise the Nucleotide of sufficient amount, as 100 to 1,500 base pairs, preferred 400 to 1,500 base pairs, most preferably 800 to 1,500 base pairs, it and corresponding target sequence height homology, thereby the possibility of raising homologous recombination.Described integrated element can be the target sequence homologous sequence in any and described host cell gene group.In addition, described integrated element can be non-code-shaped or code-shaped nucleotide sequence.On the other hand, described carrier can be incorporated into the genome of described host cell by non-homogeneous reorganization.
For self-replicating, described carrier also can comprise replication orgin, and it makes described carrier self-replicating in the purpose host cell.The example of bacterium replication orgin is plasmid pBR322, pUC19, the replication orgin of pACYC177 and pACYC184 (can duplicate in intestinal bacteria) and plasmid pUB110, pE194, the replication orgin of pTA1060 and pAMss1 (can duplicate in genus bacillus).Described replication orgin can have sudden change (to see for example Ehrlich so that its function in host cell is a thermally sensitized (temperature-sensitive), 1978, Proceedings of the National Academy of Sciences USA 75:1433).
The more than one copy of nucleotide sequence of the present invention can be inserted into described host cell, improves the output (product) of described gene product.Increase the copy number of described nucleotide sequence, can obtain in the following way: at least one other copy of described sequence is incorporated into described host cell gene group, if or cell contains the selected marker of the copy of amplification, can be by (amplifiable) selected marker thing gene of an amplification be incorporated in (included) described nucleotide sequence, thereby can select the additional copy of described nucleotide sequence by when having appropriate selection reagent, cultivating described cell.
Be used for connecting method that said elements makes up recombinant expression vector of the present invention to (see for example Sambrook etc., 1989, see above) as well known to those skilled in the art.
Carrier is introduced bacterial host cell, can be for example, (for example see with protoplast transformation, Chang and Cohen, 1979, Molecular General Genetics 168:111-115), (for example see with competent cell, Young and Spizizin, 1961, Journal of Bacteriology 81:823-829, or Dubnau and Davidoff-Abelson, 1971, Journal of Molecular Biology 56:209-221), electroporation (is for example seen Shigekawa and Dower, 1988, or connection method (conjugation) (is for example seen Koehler and Thorne Biotechniques 6:742-751),, 1987, Journal of Bacteriology 169:5771-5278) carry out.
The preferred embodiment of the invention relates to the host cell of first aspect, and wherein said heterologous gene is comprised in operon, in the preferred polycistronic operon.Term in the context of the invention " operon " refers to comprise the polynucleotide of several genes, and described gene is to cluster (cluster) and may even transcribe together becomes polycistronic mRNA, for example the gene of the enzyme of encoding metabolic pathway.Transcribing of described operon can be from promoter region, and is closed on the control of the regulatory gene of coding modulin, and the manipulation sequence that described modulin is attached to described operon conversely suppresses respectively or strengthen to transcribe.This gene or operon can be carried on the suitable plasmid of (maintained) that can stablize reservation, for example, can self-replacation stably in host cell (selection of plasmid depends on the consistency of the host cell that described plasmid and this plasmid be introduced into usually), perhaps described gene or operon can be carried on described host's the karyomit(e).Described gene can be that host cell is endogenous, and in this case, this interested product is the albumen by the natural generation of described host cell, and under most situation, this gene can be in the normal position on the karyomit(e).The described gene of product interested is a foreign gene if encode, and this gene can be carried by suitable plasmid, perhaps is integrated on the host chromosome.In one embodiment of the invention, eubacterium (eubacterium) is the eubacterium of reorganization.In another embodiment, described interested product also can be recombinant protein.
Described interested product is the product of any gene product or pathways metabolism, and it is industrial available, and can prepare in bacterial cell such as Bacillus licheniformis.
In the preferred embodiment, described heterologous polypeptide is antimicrobial peptide or fusogenic peptide, and this fusogenic peptide comprises the peptide moiety that its natural form has antimicrobial acivity.
In another preferred embodiment, described heterologous polypeptide has biosynthetic activity, and produces compound of interest or intermediate (intermediate).
Another embodiment relates to the host cell of first aspect, and wherein said compound of interest or intermediate comprise VITAMIN, amino acid, and microbiotic, carbohydrate, or tensio-active agent, and preferred described carbohydrate comprises hyaluronic acid.
In the preferred embodiment, described heterologous polypeptide is an enzyme, and described enzyme particularly is selected from the enzyme of following enzyme: oxydo-reductase (EC 1), transferring enzyme (EC 2), lytic enzyme (EC 3), lyase (EC 4), isomerase (EC 5), and ligase enzyme (EC 6).Preferred described enzyme is to have the activity that is selected from following enzyme: aminopeptidase, amylase, amyloglucosidase, mannase, carbohydrase, carboxypeptidase, catalase; Cellulase, chitinase (chitinase), at, Maltose 4-glucosyltransferase (cyclodextringlycosyltransferase), deoxyribonuclease, esterase, tilactase, beta-galactosidase enzymes, glucoamylase, glucose oxidase, Polyglucosidase, haloperoxidase (haloperoxidase), hemicellulase, saccharase, isomerase, laccase, ligase enzyme, lipase, lyase, mannosidase, oxydase, polygalacturonase, peroxidase, phytase (phytase), phenol oxidase, polyphenoloxidase, proteolytic enzyme, rnase, transferring enzyme, trans-glutaminases (transglutaminase) and zytase.Preferred described enzyme is amylase or mannase.
A second aspect of the present invention relates to the method for at least a product interested of preparation in Bacillus licheniformis mutant host cell, be included in and cultivate the Bacillus licheniformis mutant host cell that first aspect present invention limited in the suitable medium, from wherein having prepared described product.An embodiment relates to the method for second aspect, and it also comprises the described interested product of isolated or purified.The substratum of be fit to cultivating is described below, and the purifying or the method for separating prepared product, is the optional additional step of the inventive method.
Among the preparation method of the present invention, described cell is in the nutritional medium that is fit to the described polypeptide of preparation, cultivate with means known in the art.For example; described cell can be cultivated by shaking bottle (shake flask); on a small scale or large scale fermentation (comprise continuously, (batch), (fed-batch) or solid state fermentation in batches) in batches in laboratory or industrial fermentation jar, in suitable medium and make under described expression of polypeptides and/or the isolating condition and carry out this cultivation.Described cultivation is carried out with methods known in the art in suitable nutritional medium, and this substratum comprises carbon and nitrogenous source and inorganic salt.Suitable medium is from supplier (commercial suppliers) or can be according to disclosed preparation of compositions (for example catalogue of American type culture collection (the American Type Culture Collection)).If described polypeptide is secreted in the described nutritional medium, can directly reclaim this polypeptide from described substratum.If described polypeptide is not secreted, can reclaim it from cell lysate.
The substratum that is used to cultivate described cell can be the substratum of any suitable described host cell growth commonly used, and as minimal medium (minimal medium) or compound (complex) substratum, it comprises suitable fill-in (supplement).Suitable medium is from supplier or can prepare (for example catalogue of American type culture collection) according to disclosed prescription (recipes).This substratum (is for example seen bacterium and zymic book of reference (references), Bennett, J.W. and LaSure with the methods known in the art preparation, L., editors, More Gene Manipulations in Fungi, Academic Press, CA, 199l).
This polypeptide can detect this polypeptide specific method with known in the art.These detection methods can comprise uses specific antibody, forms the enzyme product, or the disappearance of enzyme substrates.For example, the activity of polypeptide described herein is determined in available enzyme test (assay).
The gained polypeptide can reclaim with methods known in the art.For example, available common method includes but not limited to, centrifugal, filtration, extraction, spraying drying (spray-drying), evaporation or precipitation reclaim described polypeptide from nutritional medium.
Polypeptide of the present invention can be with multiple means known in the art purifying, described method includes but not limited to, chromatography (for example, ion exchange chromatography, affinity chromatography, hydrophobic chromatography, chromatofocusing (chromatofocusing) and volume removing chromatogram (size exclusion)), electrophoresis method (isoelectrofocusing of for example preparation type (preparativeisoelectric focusing)), difference solubleness (differential solubility) (for example, ammonium sulfate precipitation), SDS-PAGE, or extract and (for example see, Protein Purification, J.-C.Janson and LarsRyden, editors, VCH Publishers, New York, 1989).
Third aspect present invention relates to the Bacillus licheniformis mutant host cell that first aspect limits, purposes at least a interested product of preparation, it is included in and cultivates described mutant host cell in the suitable culture medium, thereby prepare described product, and the prepared product of optional isolated or purified.
Sequence table
＜110〉Novozymes Company (Novozymes A/S)
Michael .D. Lars Ma Sen
Dave Steen .T. Qiao Gensen
Peter .B. Mancur Olson
Zhan Si .T. Anderson
＜120〉Gai Liang genus bacillus host cell
<130>10196.204
<160>84
<170>PatentIn?version?3.2
<210>1
<211>4325
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(3)..(3836)
<400>1
tg?gaa?caa?gta?gcg?gct?ttt?ccg?gaa?gta?gaa?aaa?gtg?ctt?ccg?aac 47
Glu?Gln?Val?Ala?Ala?Phe?Pro?Glu?Val?Glu?Lys?Val?Leu?Pro?Asn
1 5 10 15
gaa?aaa?agg?cag?ctg?atc?aaa?ccg?act?aaa?cag?tct?gtg?aaa?aaa?tca 95
G1u?Lys?Arg?Gln?Leu?Ile?Lys?Pro?Thr?Lys?Gln?Ser?Val?Lys?Lys?Ser
20 25 30
gca?gcc?aag?gat?gaa?aaa?gaa?atc?gaa?tgg?aat?atc?aat?cga?gtc?gac 143
Ala?Ala?Lys?Asp?Glu?Lys?Glu?Ile?Glu?Trp?Asn?Ile?Asn?Arg?Val?Asp
35 40 45
gca?cca?aaa?gcc?tgg?aaa?ttg?gga?tat?gac?gga?tcg?ggc?acg?gtt?gtc 191
Ala?Pro?Lys?Ala?Trp?Lys?Leu?Gly?Tyr?Asp?Gly?Ser?Gly?Thr?Val?Val
50 55 60
gcg?tcg?att?gat?acg?ggc?gtg?cag?tgg?gat?cat?ccg?gcc?ttg?aaa?gaa 239
Ala?Ser?Ile?Asp?Thr?Gly?Val?Gln?Trp?Asp?His?Pro?Ala?Leu?Lys?Glu
65 70 75
aaa?tac?cgc?gga?tat?gac?ccg?cag?cat?ccc?gat?cag?cct?aac?cac?gaa 287
Lys?Tyr?Arg?Gly?Tyr?Asp?Pro?Gln?His?Pro?Asp?Gln?Pro?Asn?His?Glu
80 85 90 95
ttc?agt?tgg?tat?gat?gcg?gtt?tca?ggc?gct?tct?gaa?cca?ttt?gac?gat 335
Phe?Ser?Trp?Tyr?Asp?Ala?Val?Ser?Gly?Ala?Ser?Glu?Pro?Phe?Asp?Asp
100 105 110
ctg?gag?cac?ggc?acg?cat?gta?acc?ggg?acg?atg?gtc?ggc?tca?gag?cct 383
Leu?Glu?His?Gly?Thr?His?Val?Thr?Gly?Thr?Met?Val?Gly?Ser?Glu?Pro
115 120 125
gac?ggc?caa?aac?caa?atc?ggc?gtg?gcg?ccg?ggt?gcg?aag?tgg?att?gct 431
Asp?Gly?Gln?Asn?Gln?Ile?Gly?Val?Ala?Pro?Gly?Ala?Lys?Trp?Ile?Ala
130 135 140
gtg?aaa?gct?ttt?tca?gat?gac?ggc?ggt?acg?gac?gaa?gac?ctt?att?gca 479
Val?Lys?Ala?Phe?Ser?Asp?Asp?Gly?Gly?Thr?Asp?Glu?Asp?Leu?Ile?Ala
145 150 155
gca?ggt?gaa?tgg?att?ttg?gcg?cca?aaa?gat?aaa?gac?ggc?aac?ccg?cac 527
Ala?Gly?Glu?Trp?Ile?Leu?Ala?Pro?Lys?Asp?Lys?Asp?Gly?Asn?Pro?His
160 165 170 175
cct?gaa?atg?gcg?ccg?gat?gtc?gtc?aat?aac?tca?tgg?tca?ggg?gga?gcc 575
Pro?Glu?Met?Ala?Pro?Asp?Val?Val?Asn?Asn?Ser?Trp?Ser?Gly?Gly?Ala
180 185 190
ggg?att?gat?gag?ttt?tac?agg?gat?atc?gta?aaa?gcg?tgg?cga?gct?gcc 623
Gly?Ile?Asp?Glu?Phe?Tyr?Arg?Asp?Ile?Val?Lys?Ala?Trp?Arg?Ala?Ala
195 200 205
ggc?atc?ttt?cct?gag?ttc?tct?gcg?ggg?aac?gtc?gat?ttc?gcc?aat?ccg 671
Gly?Ile?Phe?Pro?Glu?Phe?Ser?Ala?Gly?Asn?Val?Asp?Phe?Ala?Asn?Pro
210 215 220
gga?gga?cct?gct?tcg?att?gcc?aat?ccg?gca?aac?tac?cct?gaa?gcg?ttc 719
Gly?Gly?Pro?Ala?Ser?Ile?Ala?Asn?Pro?Ala?Asn?Tyr?Pro?Glu?Ala?Phe
225 230 235
gca?acc?gga?gcg?aca?gac?atc?gat?aat?cgc?tta?gcc?gat?ttt?tca?ctt 767
Ala?Thr?Gly?Ala?Thr?Asp?Ile?Asp?Asn?Arg?Leu?Ala?Asp?Phe?Ser?Leu
240 245 250 255
caa?ggt?cct?tcc?ccg?tat?gat?gag?act?aag?cct?gaa?ata?tcg?gcg?cct 815
Gln?Gly?Pro?Ser?Pro?Tyr?Asp?Glu?Thr?Lys?Pro?Glu?Ile?Ser?Ala?Pro
260 265 270
ggt?gtc?aac?att?cgc?tca?tct?gtg?cct?gga?agc?ggc?tat?caa?gac?ggc 863
Gly?Val?Asn?Ile?Arg?Ser?Ser?Val?Pro?Gly?Ser?Gly?Tyr?Gln?Asp?Gly
275 280 285
tgg?gac?ggc?act?tca?atg?gct?ggc?ccg?cat?gtc?gca?gcc?gcc?gcc?gct 911
Trp?Asp?Gly?Thr?Ser?Met?Ala?Gly?Pro?His?Val?Ala?Ala?Ala?Ala?Ala
290 295 300
ctt?atc?aag?caa?gcc?gat?tcg?tcg?atc?act?gtt?gat?gag?acc?gaa?aag 959
Leu?Ile?Lys?Gln?Ala?Asp?Ser?Ser?Ile?Thr?Val?Asp?Glu?Thr?Glu?Lys
305 310 315
att?ttg?atg?gaa?act?gca?aca?ccg?ctc?aca?gac?agc?aaa?ttt?acc?gaa 1007
Ile?Leu?Met?Glu?Thr?Ala?Thr?Pro?Leu?Thr?Asp?Ser?Lys?Phe?Thr?Glu
320 325 330 335
tcg?ccg?aac?aac?gga?tac?ggc?cac?gga?ctc?gtg?aac?gtg?ttt?gac?gca 1055
Ser?Pro?Asn?Asn?Gly?Tyr?Gly?His?Gly?Leu?Val?Asn?Val?Phe?Asp?Ala
340 345 350
gtg?tcc?gct?gtg?aca?gac?ggg?ttg?ggc?aga?gcg?gaa?gga?caa?gtc?gga 1103
Val?Ser?Ala?Val?Thr?Asp?Gly?Leu?Gly?Arg?Ala?Glu?Gly?Gln?Val?Gly
355 360 365
aaa?gaa?ggc?gaa?gac?aaa?agt?ccg?ccg?gcc?tta?aac?cat?caa?gag?atc 1151
Lys?Glu?Gly?Glu?Asp?Lys?Ser?Pro?Pro?Ala?Leu?Asn?His?Gln?Glu?Ile
370 375 380
acc?gaa?att?tac?tca?ggc?tcg?gaa?acg?cct?ctt?aaa?gct?gat?gta?gaa 1199
Thr?Glu?Ile?Tyr?Ser?Gly?Ser?Glu?Thr?Pro?Leu?Lys?Ala?Asp?Val?Glu
385 390 395
gac?gat?gtc?agc?atc?ata?tcg?gtc?aaa?ttg?agc?tat?aaa?aca?gat?gac 1247
Asp?Asp?Val?Ser?Ile?Ile?Ser?Val?Lys?Leu?Ser?Tyr?Lys?Thr?Asp?Asp
400 405 410 415
gcc?gat?tgg?gag?acg?atc?gct?gcc?aaa?caa?act?tcc?ggc?gac?tat?aaa 1295
Ala?Asp?Trp?Glu?Thr?Ile?Ala?Ala?Lys?Gln?Thr?Ser?Gly?Asp?Tyr?Lys
420 425 430
aaa?gga?acg?tac?gag?gcg?gct?gta?act?gtt?tct?gaa?ggg?aaa?acc?ctt 1343
Lys?Gly?Thr?Tyr?Glu?Ala?Ala?Val?Thr?Val?Ser?Glu?Gly?Lys?Thr?Leu
435 440 445
tcc?tac?aag?tgg?att?gtc?acc?gat?ttc?gga?ggc?aac?aaa?acg?gaa?tcc 1391
Ser?Tyr?Lys?Trp?Ile?Val?Thr?Asp?Phe?Gly?Gly?Asn?Lys?Thr?Glu?Ser
450 455 460
aaa?gtg?tac?gaa?gtc?ccg?att?tcc?ccg?gct?gtg?aca?aca?ggg?tat?aaa 1439
Lys?Val?Tyr?Glu?Val?Pro?Ile?Ser?Pro?Ala?Val?Thr?Thr?Gly?Tyr?Lys
465 470 475
caa?gat?ttc?gag?aat?tcc?gcc?agc?ggc?tgg?ctc?aca?tca?ggc?gtg?aag 1487
Gln?Asp?Phe?Glu?Asn?Ser?Ala?Ser?Gly?Trp?Leu?Thr?Ser?Gly?Val?Lys
480 485 490 495
aat?tca?tgg?gag?cgc?gga?att?cct?cag?tca?ggc?ccg?aac?gcc?gca?gca 1535
Asn?Ser?Trp?Glu?Arg?Gly?Ile?Pro?Gln?Ser?Gly?Pro?Asn?Ala?Ala?Ala
500 505 510
tcg?gga?aaa?aac?gta?ttc?gca?aca?aat?ctg?aca?ggg?ccg?tat?gaa?agt 1583
Ser?Gly?Lys?Asn?Val?Phe?Ala?Thr?Asn?Leu?Thr?Gly?Pro?Tyr?Glu?Ser
515 520 525
tcg?gct?aat?atg?aat?ctt?ctc?atg?cct?cct?gtc?agc?gtt?cca?aaa?aat 1631
Ser?Ala?Asn?Met?Asn?Leu?Leu?Met?Pro?Pro?Val?Ser?Val?Pro?Lys?Asn
530 535 540
caa?aag?ctt?tat?ctc?aca?tac?aaa?tat?tgg?cgc?gat?att?gaa?gag?gac 1679
Gln?Lys?Leu?Tyr?Leu?Thr?Tyr?Lys?Tyr?Trp?Arg?Asp?Ile?Glu?Glu?Asp
545 550 555
ttt?gat?tac?ggc?ttc?gtt?tac?gta?cag?cct?gaa?gga?aaa?ggt?gaa?tgg 1727
Phe?Asp?Tyr?Gly?Phe?Val?Tyr?Val?Gln?Pro?Glu?Gly?Lys?Gly?Glu?Trp
560 565 570 575
atc?ccg?gct?gcc?gag?tac?agc?ggc?aag?aca?tca?gaa?tgg?aaa?gac?gga 1775
Ile?Pro?Ala?Ala?Glu?Tyr?Ser?Gly?Lys?Thr?Ser?Glu?Trp?Lys?Asp?Gly
580 585 590
cag?atc?gat?ttg?tcg?gaa?tac?ggg?gga?cag?acg?att?aag?gtc?atg?ttc 1823
Gln?Ile?Asp?Leu?Ser?Glu?Tyr?Gly?Gly?Gln?Thr?Ile?Lys?Val?Met?Phe
595 600 605
aac?ctt?caa?tct?gac?gac?agc?att?gaa?ggt?gac?ggg?ctg?tac?atc?gat 1871
Asn?Leu?Gln?Ser?Asp?Asp?Ser?Ile?Glu?Gly?Asp?Gly?Leu?Tyr?Ile?Asp
610 615 620
gat?gta?gcg?ctt?gtc?aag?gaa?gtg?aag?agc?gcc?ggt?acg?aaa?aaa?cga 1919
Asp?Val?Ala?Leu?Val?Lys?Glu?Val?Lys?Ser?Ala?Gly?Thr?Lys?Lys?Arg
625 630 635
ttg?ggc?gtt?gaa?aaa?cag?ccg?gcc?aaa?atg?aag?gat?aag?aaa?aca?aag 1967
Leu?Gly?Val?Glu?Lys?Gln?Pro?Ala?Lys?Met?Lys?Asp?Lys?Lys?Thr?Lys
640 645 650 655
aaa?cga?atg?att?gat?ccg?aaa?aaa?gca?aaa?ccg?gca?gaa?gcc?ctt?cag 2015
Lys?Arg?Met?Ile?Asp?Pro?Lys?Lys?Ala?Lys?Pro?Ala?Glu?Ala?Leu?Gln
660 665 670
gaa?aaa?acc?gaa?acg?aaa?aaa?gca?gct?cct?gcg?gtt?ctt?ccg?gtg?cgc 2063
Glu?Lys?Thr?Glu?Thr?Lys?Lys?Ala?Ala?Pro?Ala?Val?Leu?Pro?Val?Arg
675 680 685
gct?cag?gtc?agc?gtg?ctg?gaa?acg?ggc?aaa?tcg?aca?tat?tcc?aac?cag 2111
Ala?Gln?Val?Ser?Val?Leu?Glu?Thr?Gly?Lys?Ser?Thr?Tyr?Ser?Asn?Gln
690 695 700
gca?aca?ggc?gca?tac?agc?ttg?gct?cat?gca?cct?gga?acc?tat?acg?ctg 2159
Ala?Thr?Gly?Ala?Tyr?Ser?Leu?Ala?His?Ala?Pro?Gly?Thr?Tyr?Thr?Leu
705 710 715
aaa?gcg?gaa?gcg?tac?gga?tat?gaa?tca?acc?gcc?caa?acc?gta?aaa?atc 2207
Lys?Ala?Glu?Ala?Tyr?Gly?Tyr?Glu?Ser?Thr?Ala?Gln?Thr?Val?Lys?Ile
720 725 730 735
gag?tcc?gac?aag?aca?aca?acg?gca?gac?ttt?gtt?tta?aaa?gaa?cta?aaa 2255
Glu?Ser?Asp?Lys?Thr?Thr?Thr?Ala?Asp?Phe?Val?Leu?Lys?Glu?Leu?Lys
740 745 750
aaa?ggg?acg?ctc?acc?gga?acg?ata?aaa?aat?aaa?aaa?acg?ggc?gaa?ccc 2303
Lys?Gly?Thr?Leu?Thr?Gly?Thr?Ile?Lys?Asn?Lys?Lys?Thr?Gly?Glu?Pro
755 760 765
gtc?cgg?cat?gca?aag?ttg?tat?atc?gtt?gaa?gat?gca?gct?gta?aaa?cca 2351
Val?Arg?His?Ala?Lys?Leu?Tyr?Ile?Val?Glu?Asp?Ala?Ala?Val?Lys?Pro
770 775 780
gtc?cag?acg?gat?gat?gac?gga?agc?tat?tca?tta?acg?gcc?tat?gaa?ggc 2399
Val?Gln?Thr?Asp?Asp?Asp?Gly?Ser?Tyr?Ser?Leu?Thr?Ala?Tyr?Glu?Gly
785 790 795
tcg?tat?acg?gta?aag?gtc?tct?gca?aac?gga?tat?tac?agc?agc?gag?ttt 2447
Ser?Tyr?Thr?Val?Lys?Val?Ser?Ala?Asn?Gly?Tyr?Tyr?Ser?Ser?Glu?Phe
800 805 810 815
tcc?gtt?gat?tta?aaa?ggc?gat?gtc?tcg?aaa?gat?atc?gat?ctc?gat?cct 2495
Ser?Val?Asp?Leu?Lys?Gly?Asp?Val?Ser?Lys?Asp?Ile?Asp?Leu?Asp?Pro
820 825 830
ttc?atc?ggc?tat?ccg?ggg?gaa?ata?ggc?tat?gat?gac?gga?acc?ggg?gaa 2543
Phe?Ile?Gly?Tyr?Pro?Gly?Glu?Ile?Gly?Tyr?Asp?Asp?Gly?Thr?Gly?Glu
835 840 845
aac?gcc?tgg?gct?ttc?tat?gaa?tcc?ggc?aac?ggg?ctg?gcg?gtt?aaa?atg 2591
Asn?Ala?Trp?Ala?Phe?Tyr?Glu?Ser?Gly?Asn?Gly?Leu?Ala?Val?Lys?Met
850 855 860
acg?ctt?gaa?aac?ggg?cag?gaa?aaa?gcc?atg?ctg?aaa?ggc?ggt?ttg?ttt 2639
Thr?Leu?Glu?Asn?Gly?Gln?Glu?Lys?Ala?Met?Leu?Lys?Gly?Gly?Leu?Phe
865 870 875
aaa?ttc?tgg?gat?aca?gaa?ttc?cct?gat?ccg?ggc?ggc?act?gat?ttt?gca 2687
Lys?Phe?Trp?Asp?Thr?Glu?Phe?Pro?Asp?Pro?Gly?Gly?Thr?Asp?Phe?Ala
880 885 890 895
gta?gag?gtc?tat?gat?gct?tcg?ggt?gaa?aaa?ggc?tct?ccg?ggc?aaa?aaa 2735
Val?Glu?Val?Tyr?Asp?Ala?Ser?Gly?Glu?Lys?Gly?Ser?Pro?Gly?Lys?Lys
900 905 910
atc?gcc?ggc?ccg?ttc?aag?gct?gaa?gcg?ctc?cgc?aca?ggc?gaa?tgg?acg 2783
Ile?Ala?Gly?Pro?Phe?Lys?Ala?Glu?Ala?Leu?Arg?Thr?Gly?Glu?Trp?Thr
915 920 925
acc?gtt?gat?tta?ggt?gac?gaa?ggc?atc?atc?gtc?gga?aaa?gac?ttt?tac 2831
Thr?Val?Asp?Leu?Gly?Asp?Glu?Gly?Ile?Ile?Val?Gly?Lys?Asp?Phe?Tyr
930 935 940
ctc?gta?tac?gtc?cag?aaa?gag?gat?ctg?gcc?aac?tca?cct?gga?ctt?gct 2879
Leu?Val?Tyr?Val?Gln?Lys?Glu?Asp?Leu?Ala?Asn?Ser?Pro?Gly?Leu?Ala
945 950 955
aca?gac?gaa?gac?gga?gaa?tat?tcc?ggc?cgc?aac?tgg?cag?tat?acg?gac 2927
Thr?Asp?Glu?Asp?Gly?Glu?Tyr?Ser?Gly?Arg?Asn?Trp?Gln?Tyr?Thr?Asp
960 965 970 975
ggc?tca?tgg?tca?aaa?gcg?cca?tca?gac?caa?ggc?aac?ttt?atg?atc?cgc 2975
Gly?Ser?Trp?Ser?Lys?Ala?Pro?Ser?Asp?Gln?Gly?Asn?Phe?Met?Ile?Arg
980 985 990
gct?ttg?gtc?gac?tat?gaa?ttg?tcg?gtg?cct?gtg?att?acg?tct?cct?aaa 3023
Ala?Leu?Val?Asp?Tyr?Glu?Leu?Ser?Val?Pro?Val?Ile?Thr?Ser?Pro?Lys
995 1000 1005
gac?gga?ttc?atc?acc?aac?caa?aag?aac?gct?gtc?att?gaa?ggg?aca 3068
Asp?Gly?Phe?Ile?Thr?Asn?Gln?Lys?Asn?Ala?Val?Ile?Glu?Gly?Thr
1010 1015 1020
tcc?tcg?ccg?aat?acg?acc?gtt?cat?ctc?ttt?aac?ggg?gat?gaa?gaa 3113
Ser?Ser?Pro?Asn?Thr?Thr?Val?His?Leu?Phe?Asn?Gly?Asp?G1u?Glu
1025 1030 1035
gcc?gga?aca?gcg?gaa?acg?gcg?gca?gac?ggc?act?ttc?tca?aaa?gaa 3158
Ala?Gly?Thr?Ala?Glu?Thr?Ala?Ala?Asp?Gly?Thr?Phe?Ser?Lys?Glu
1040 1045 1050
ata?ccg?ttg?aat?aaa?ggg?gaa?aat?gtc?atc?acc?gcg?aaa?tcg?tca 3203
Ile?Pro?Leu?Asn?Lys?Gly?Glu?Asn?Val?Ile?Thr?Ala?Lys?Ser?Ser
1055 1060 1065
tcg?gct?tcc?gga?acg?act?gat?gct?tca?gaa?ccg?gtc?cgg?atc?gtc 3248
Ser?Ala?Ser?Gly?Thr?Thr?Asp?Ala?Ser?Glu?Pro?Val?Arg?Ile?Val
1070 1075 1080
ctt?gac?cag?aaa?aag?ccg?aag?ctt?aca?atc?gac?acg?ccg?gaa?agc 3293
Leu?Asp?Gln?Lys?Lys?Pro?Lys?Leu?Thr?Ile?Asp?Thr?Pro?Glu?Ser
1085 1090 1095
ggc?tca?aaa?ctc?aat?aaa?gag?acc?gtc?acc?gtg?aaa?gga?aca?gta 3338
Gly?Ser?Lys?Leu?Asn?Lys?Glu?Thr?Val?Thr?Val?Lys?Gly?Thr?Val
1100 1105 1110
tca?gac?gat?cac?tta?gaa?tct?gtt?cat?gta?aac?ggc?aaa?aaa?gca 3383
Ser?Asp?Asp?His?Leu?Glu?Ser?Val?His?Val?Asn?Gly?Lys?Lys?Ala
1115 1120 1125
gct?gtt?gac?aat?ggc?gaa?tat?tct?gca?agg?att?atg?ctt?gac?aac 3428
Ala?Val?Asp?Asn?Gly?Glu?Tyr?Ser?Ala?Arg?Ile?Met?Leu?Asp?Asn
1130 1135 1140
ggc?aaa?aat?gaa?atc?aaa?gta?acg?gca?tcg?gat?gcg?gca?ggc?aac 3473
Gly?Lys?Asn?Glu?Ile?Lys?Val?Thr?Ala?Ser?Asp?Ala?Ala?Gly?Asn
1145 1150 1155
aaa?acg?acc?aaa?aag?gtc?acg?gta?gat?gtc?aac?ttt?gaa?gcg?ccg 3518
Lys?Thr?Thr?Lys?Lys?Val?Thr?Val?Asp?Val?Asn?Phe?Glu?Ala?Pro
1160 1165 1170
caa?atc?acc?ggc?ttg?aaa?ccg?gcc?gaa?gat?ctc?gag?ctc?aaa?acg 3563
Gln?Ile?Thr?Gly?Leu?Lys?Pro?Ala?Glu?Asp?Leu?Glu?Leu?Lys?Thr
1175 1180 1185
gga?gaa?acc?gtg?aaa?atc?gag?ttt?gaa?agc?gcc?gct?gat?ttg?gat 3608
Gly?Glu?Thr?Val?Lys?Ile?Glu?Phe?Glu?Ser?Ala?Ala?Asp?Leu?Asp
1190 1195 1200
gct?gtc?ttt?gtg?atc?aga?atg?ccg?ctg?acc?aat?ttc?aaa?acc?gct 3653
Ala?Val?Phe?Val?Ile?Arg?Met?Pro?Leu?Thr?Asn?Phe?Lys?Thr?Ala
1205 1210 1215
gcc?caa?aac?gta?acg?gag?ctg?ccg?atc?aga?gaa?gtc?tca?aaa?gga 3698
Ala?Gln?Asn?Val?Thr?Glu?Leu?Pro?Ile?Arg?Glu?Val?Ser?Lys?Gly
1220 1225 1230
aaa?tat?gaa?gga?tat?tgg?acc?gct?act?tca?act?gca?aaa?gca?aaa 3743
Lys?Tyr?Glu?Gly?Tyr?Trp?Thr?Ala?Thr?Ser?Thr?Ala?Lys?Ala?Lys
1235 1240 1245
gga?gcg?gaa?atc?gag?gtc?atc?gtc?aga?gat?gat?tac?ggc?aat?gaa 3788
Gly?Ala?Glu?Ile?Glu?Val?Ile?Val?Arg?Asp?Asp?Tyr?Gly?Asn?Glu
1250 1255 1260
acg?aga?caa?acg?gca?aaa?ggc?aag?ctg?tat?atc?aat?gaa?aag?ctg 3833
Thr?Arg?Gln?Thr?Ala?Lys?Gly?Lys?Leu?Tyr?Ile?Asn?Glu?Lys?Leu
1265 1270 1275
aaa?taaaggtgaa?aagacgctgt?ctttaatggc?agcgtttttt?tcgttttacg 3886
Lys
atcgacaaat?tcagtacgaa?aacttcaaaa?aatgtacgat?ttacgcaaca?ttaattgaca?3946
gactttacct?ttgggcttga?tttatactta?ggaaaacaaa?cactaaggtc?accgagccgc?4006
agaaagggga?aggatgtgga?aatctattta?gatgcgatat?ggctgttaaa?cttttgtttt?4066
gacttgctgc?ttttaatgat?gaccgcattt?attttaaagc?gaagggttaa?aaagcggagg?4126
ctgatcctag?gggcatttgt?cgcgtcaagc?atcgttctgt?ttatgtttac?acctttttca?4186
ccgtacgtcc?ttcatcctgc?cggcaaactg?tcgttttcgg?ttgtgatcgt?tcttgtggca?4246
tttggtttta?agcggttccg?gttttttttg?cagaatttgt?tttcttttta?ttttgccact?4306
tttttaatgg?gaggaggga 4325
<210>2
<211>1278
<212>PRT
＜213〉Bacillus licheniformis
<400>2
Glu?Gln?Val?Ala?Ala?Phe?Pro?Glu?Val?Glu?Lys?Val?Leu?Pro?Asn?Glu
1 5 10 15
Lys?Arg?Gln?Leu?Ile?Lys?Pro?Thr?Lys?Gln?Ser?Val?Lys?Lys?Ser?Ala
20 25 30
Ala?Lys?Asp?Glu?Lys?Glu?Ile?Glu?Trp?Asn?Ile?Asn?Arg?Val?Asp?Ala
35 40 45
Pro?Lys?Ala?Trp?Lys?Leu?Gly?Tyr?Asp?Gly?Ser?Gly?Thr?Val?Val?Ala
50 55 60
Ser?Ile?Asp?Thr?Gly?Val?Gln?Trp?Asp?His?Pro?Ala?Leu?Lys?Glu?Lys
65 70 75 80
Tyr?Arg?Gly?Tyr?Asp?Pro?Gln?His?Pro?Asp?Gln?Pro?Asn?His?Glu?Phe
85 90 95
Ser?Trp?Tyr?Asp?Ala?Val?Ser?Gly?Ala?Ser?Glu?Pro?Phe?Asp?Asp?Leu
100 105 110
Glu?His?Gly?Thr?His?Val?Thr?Gly?Thr?Met?Val?Gly?Ser?Glu?Pro?Asp
115 120 125
Gly?Gln?Asn?Gln?Ile?Gly?Val?Ala?Pro?Gly?Ala?Lys?Trp?Ile?Ala?Val
130 135 140
Lys?Ala?Phe?Ser?Asp?Asp?Gly?Gly?Thr?Asp?Glu?Asp?Leu?Ile?Ala?Ala
145 150 155 160
Gly?Glu?Trp?Ile?Leu?Ala?Pro?Lys?Asp?Lys?Asp?Gly?Asn?Pro?His?Pro
165 170 l75
Glu?Met?Ala?Pro?Asp?Val?Val?Asn?Asn?Ser?Trp?Ser?Gly?Gly?Ala?Gly
180 185 190
Ile?Asp?Glu?Phe?Tyr?Arg?Asp?Ile?Val?Lys?Ala?Trp?Arg?Ala?Ala?Gly
195 200 205
Ile?Phe?Pro?Glu?Phe?Ser?Ala?Gly?Asn?Val?Asp?Phe?Ala?Asn?Pro?Gly
210 215 220
Gly?Pro?Ala?Ser?Ile?Ala?Asn?Pro?Ala?Asn?Tyr?Pro?Glu?Ala?Phe?Ala
225 230 235 240
Thr?Gly?Ala?Thr?Asp?Ile?Asp?Asn?Arg?Leu?Ala?Asp?Phe?Ser?Leu?Gln
245 250 255
Gly?Pro?Ser?Pro?Tyr?Asp?Glu?Thr?Lys?Pro?Glu?Ile?Ser?Ala?Pro?Gly
260 265 270
Val?Asn?Ile?Arg?Ser?Ser?Val?Pro?Gly?Ser?Gly?Tyr?Gln?Asp?Gly?Trp
275 280 285
Asp?Gly?Thr?Ser?Met?Ala?Gly?Pro?His?Val?Ala?Ala?Ala?Ala?Ala?Leu
290 295 300
Ile?Lys?Gln?Ala?Asp?Ser?Ser?Ile?Thr?Val?Asp?Glu?Thr?Glu?Lys?Ile
305 310 315 320
Leu?Met?Glu?Thr?Ala?Thr?Pro?Leu?Thr?Asp?Ser?Lys?Phe?Thr?Glu?Ser
325 330 335
Pro?Asn?Asn?Gly?Tyr?Gly?His?Gly?Leu?Val?Asn?Val?Phe?Asp?Ala?Val
340 345 350
Ser?Ala?Val?Thr?Asp?Gly?Leu?Gly?Arg?Ala?Glu?Gly?Gln?Val?Gly?Lys
355 360 365
Glu?Gly?Glu?Asp?Lys?Ser?Pro?Pro?Ala?Leu?Asn?His?Gln?Glu?Ile?Thr
370 375 380
Glu?Ile?Tyr?Ser?Gly?Ser?Glu?Thr?Pro?Leu?Lys?Ala?Asp?Val?Glu?Asp
385 390 395 400
Asp?Val?Ser?Ile?Ile?Ser?Val?Lys?Leu?Ser?Tyr?Lys?Thr?Asp?Asp?Ala
405 410 415
Asp?Trp?Glu?Thr?Ile?Ala?Ala?Lys?Gln?Thr?Ser?Gly?Asp?Tyr?Lys?Lys
420 425 430
Gly?Thr?Tyr?Glu?Ala?Ala?Val?Thr?Val?Ser?Glu?Gly?Lys?Thr?Leu?Ser
435 440 445
Tyr?Lys?Trp?Ile?Val?Thr?Asp?Phe?Gly?Gly?Asn?Lys?Thr?Glu?Ser?Lys
450 455 460
Val?Tyr?Glu?Val?Pro?Ile?Ser?Pro?Ala?Val?Thr?Thr?Gly?Tyr?Lys?Gln
465 470 475 480
Asp?Phe?Glu?Asn?Ser?Ala?Ser?Gly?Trp?Leu?Thr?Ser?Gly?Val?Lys?Asn
485 490 495
Ser?Trp?Glu?Arg?Gly?Ile?Pro?Gln?Ser?Gly?Pro?Asn?Ala?Ala?Ala?Ser
500 505 510
Gly?Lys?Asn?Val?Phe?Ala?Thr?Asn?Leu?Thr?Gly?Pro?Tyr?Glu?Ser?Ser
515 520 525
Ala?Asn?Met?Asn?Leu?Leu?Met?Pro?Pro?Val?Ser?Val?Pro?Lys?Asn?Gln
530 535 540
Lys?Leu?Tyr?Leu?Thr?Tyr?Lys?Tyr?Trp?Arg?Asp?Ile?Glu?Glu?Asp?Phe
545 550 555 560
Asp?Tyr?Gly?Phe?Val?Tyr?Val?Gln?Pro?Glu?Gly?Lys?Gly?Glu?Trp?Ile
565 570 575
Pro?Ala?Ala?Glu?Tyr?Ser?Gly?Lys?Thr?Ser?Glu?Trp?Lys?Asp?Gly?Gln
580 585 590
Ile?Asp?Leu?Ser?Glu?Tyr?Gly?Gly?Gln?Thr?Ile?Lys?Val?Met?Phe?Asn
595 600 605
Leu?Gln?Ser?Asp?Asp?Ser?Ile?Glu?Gly?Asp?Gly?Leu?Tyr?Ile?Asp?Asp
610 615 620
Val?Ala?Leu?Val?Lys?Glu?Val?Lys?Ser?Ala?Gly?Thr?Lys?Lys?Arg?Leu
625 630 635 640
Gly?Val?Glu?Lys?G1n?Pro?Ala?Lys?Met?Lys?Asp?Lys?Lys?Thr?Lys?Lys
645 650 655
Arg?Met?Ile?Asp?Pro?Lys?Lys?Ala?Lys?Pro?Ala?Glu?Ala?Leu?Gln?Glu
660 665 670
Lys?Thr?Glu?Thr?Lys?Lys?Ala?Ala?Pro?Ala?Val?Leu?Pro?Val?Arg?A1a
675 680 685
Gln?Val?Ser?Val?Leu?Glu?Thr?Gly?Lys?Ser?Thr?Tyr?Ser?Asn?Gln?Ala
690 695 700
Thr?Gly?Ala?Tyr?Ser?Leu?Ala?His?Ala?Pro?Gly?Thr?Tyr?Thr?Leu?Lys
705 710 715 720
Ala?Glu?Ala?Tyr?Gly?Tyr?Glu?Ser?Thr?Ala?Gln?Thr?Val?Lys?Ile?Glu
725 730 735
Ser?Asp?Lys?Thr?Thr?Thr?Ala?Asp?Phe?Val?Leu?Lys?Glu?Leu?Lys?Lys
740 745 750
Gly?Thr?Leu?Thr?Gly?Thr?Ile?Lys?Asn?Lys?Lys?Thr?Gly?Glu?Pro?Val
755 760 765
Arg?His?Ala?Lys?Leu?Tyr?Ile?Val?Glu?Asp?Ala?Ala?Val?Lys?Pro?Val
770 775 780
Gln?Thr?Asp?Asp?Asp?Gly?Ser?Tyr?Ser?Leu?Thr?Ala?Tyr?Glu?Gly?Ser
785 790 795 800
Tyr?Thr?Val?Lys?Val?Ser?Ala?Asn?Gly?Tyr?Tyr?Ser?Ser?Glu?Phe?Ser
805 810 815
Val?Asp?Leu?Lys?Gly?Asp?Val?Ser?Lys?Asp?Ile?Asp?Leu?Asp?Pro?Phe
820 825 830
Ile?Gly?Tyr?Pro?Gly?Glu?Ile?Gly?Tyr?Asp?Asp?Gly?Thr?Gly?Glu?Asn
835 840 845
Ala?Trp?Ala?Phe?Tyr?Glu?Ser?Gly?Asn?Gly?Leu?Ala?Val?Lys?Met?Thr
850 855 860
Leu?Glu?Asn?Gly?Gln?Glu?Lys?Ala?Met?Leu?Lys?Gly?Gly?Leu?Phe?Lys
865 870 875 880
Phe?Trp?Asp?Thr?Glu?Phe?Pro?Asp?Pro?Gly?Gly?Thr?Asp?Phe?Ala?Val
885 890 895
Glu?Val?Tyr?Asp?Ala?Ser?Gly?Glu?Lys?Gly?Ser?Pro?Gly?Lys?Lys?Ile
900 905 910
Ala?Gly?Pro?Phe?Lys?Ala?Glu?Ala?Leu?Arg?Thr?Gly?Glu?Trp?Thr?Thr
915 920 925
Val?Asp?Leu?Gly?Asp?Glu?Gly?Ile?Ile?Val?Gly?Lys?Asp?Phe?Tyr?Leu
930 935 940
Val?Tyr?Val?Gln?Lys?Glu?Asp?Leu?Ala?Asn?Ser?Pro?Gly?Leu?Ala?Thr
945 950 955 960
Asp?Glu?Asp?Gly?Glu?Tyr?Ser?Gly?Arg?Asn?Trp?Gln?Tyr?Thr?Asp?Gly
965 970 975
Ser?Trp?Ser?Lys?Ala?Pro?Ser?Asp?Gln?Gly?Asn?Phe?Met?Ile?Arg?Ala
980 985 990
Leu?Val?Asp?Tyr?Glu?Leu?Ser?Val?Pro?Val?Ile?Thr?Ser?Pro?Lys?Asp
995 1000 1005
Gly?Phe?Ile?Thr?Asn?Gln?Lys?Asn?Ala?Val?Ile?Glu?Gly?Thr?Ser
1010 1015 1020
Ser?Pro?Asn?Thr?Thr?Val?His?Leu?Phe?Asn?Gly?Asp?Glu?Glu?Ala
1025 1030 1035
Gly?Thr?Ala?Glu?Thr?Ala?Ala?Asp?Gly?Thr?Phe?Ser?Lys?Glu?Ile
1040 1045 1050
Pro?Leu?Asn?Lys?Gly?Glu?Asn?Val?Ile?Thr?Ala?Lys?Ser?Ser?Ser
1055 1060 1065
Ala?Ser?Gly?Thr?Thr?Asp?Ala?Ser?Glu?Pro?Val?Arg?Ile?Val?Leu
1070 1075 1080
Asp?Gln?Lys?Lys?Pro?Lys?Leu?Thr?Ile?Asp?Thr?Pro?Glu?Ser?Gly
1085 1090 1095
Ser?Lys?Leu?Asn?Lys?Glu?Thr?Val?Thr?Val?Lys?Gly?Thr?Val?Ser
1100 1105 1110
Asp?Asp?His?Leu?Glu?Ser?Val?His?Val?Asn?Gly?Lys?Lys?Ala?Ala
1115 1120 1125
Val?Asp?Asn?Gly?Glu?Tyr?Ser?Ala?Arg?Ile?Met?Leu?Asp?Asn?Gly
1130 1135 1140
Lys?Asn?Glu?Ile?Lys?Val?Thr?Ala?Ser?Asp?Ala?Ala?Gly?Asn?Lys
1145 1150 1155
Thr?Thr?Lys?Lys?Val?Thr?Val?Asp?Val?Asn?Phe?Glu?Ala?Pro?Gln
1160 1165 1170
Ile?Thr?Gly?Leu?Lys?Pro?Ala?Glu?Asp?Leu?Glu?Leu?Lys?Thr?Gly
1175 1180 1185
Glu?Thr?Val?Lys?Ile?Glu?Phe?Glu?Ser?Ala?Ala?Asp?Leu?Asp?Ala
1190 1195 1200
Val?Phe?Val?Ile?Arg?Met?Pro?Leu?Thr?Asn?Phe?Lys?Thr?Ala?Ala
1205 1210 1215
Gln?Asn?Val?Thr?Glu?Leu?Pro?Ile?Arg?Glu?Val?Ser?Lys?Gly?Lys
1220 1225 1230
Tyr?Glu?Gly?Tyr?Trp?Thr?Ala?Thr?Ser?Thr?Ala?Lys?Ala?Lys?Gly
1235 1240 1245
Ala?Glu?Ile?Glu?Val?Ile?Val?Arg?Asp?Asp?Tyr?Gly?Asn?Glu?Thr
1250 1255 1260
Arg?Gln?Thr?Ala?Lys?Gly?Lys?Leu?Tyr?Ile?Asn?Glu?Lys?Leu?Lys
1265 1270 1275
<210>3
<211>3405
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(2906)
<400>3
aatgatatca?aacctttcat?accgatccct?ccagtttcgt?tttgataaaa?ctagcaactc 60
tattaaactt?tcttgctcta?tcttatccca?gcaaaatgaa?aatgtttgtc?acaatgtgtg 120
tgcaaaatga?ttctagtttt?tagaagtttt?gttgaaaact?gaaggaatcg?catgattcag 180
cggatacaaa?ccatgaatgt?aacttactca?cagcttatcc?taaggataaa?cacatattac 240
ccacaggata?tatccacata?tccacatact?tattcaatat?ttagtataag?aacgtatatt 300
ccctacaata?tctatacaca?agtttattca?cttatacaca?gtaaattgtg?cataaatcta 360
gagaaattca?ctccaataca?ttgaatcttt?gaaaattatt?tctatatata?gaaggatttt 420
tttgaaactg?agagaatatt?ttaaaagttc?gaaactctaa?taattacaaa?gaaacattta 480
tccagaaggg?ggaaaacaga?ttg?aga?aaa?agt?atc?gtg?cgc?tat?ttt?gtt?atg 533
Leu?Arg?Lys?Ser?Ile?Val?Arg?Tyr?Phe?Val?Met
1 5 10
gct?ttt?att?cta?tta?ttt?gcg?tta?tcc?aca?ttc?ctc?acc?gga?gtg?cag 581
Ala?Phe?Ile?Leu?Leu?Phe?Ala?Leu?Ser?Thr?Phe?Leu?Thr?Gly?Val?Gln
15 20 25
gca?act?tcc?gtt?ccc?gat?aaa?aag?tcg?cct?gag?ctt?gag?aaa?gct?gaa 629
Ala?Thr?Ser?Val?Pro?Asp?Lys?Lys?Ser?Pro?Glu?Leu?Glu?Lys?Ala?Glu
30 35 40
atc?tac?ggt?gat?att?gat?gtg?acg?tct?gat?aaa?cag?acg?acg?gtt?atc 677
Ile?Tyr?Gly?Asp?Ile?Asp?Val?Thr?Ser?Asp?Lys?Gln?Thr?Thr?Val?Ile
45 50 55
gtg?gaa?ctg?aaa?gaa?aag?tcg?ctt?gcc?gaa?gca?aaa?gcg?gac?gga?gaa 725
Val?Glu?Leu?Lys?Glu?Lys?Ser?Leu?Ala?Glu?Ala?Lys?Ala?Asp?Gly?Glu
60 65 70 75
aaa?caa?acg?aag?gct?tct?cta?aaa?aca?gct?cga?agc?aaa?gcg?ctg?aaa 773
Lys?Gln?Thr?Lys?Ala?Ser?Leu?Lys?Thr?Ala?Arg?Ser?Lys?Ala?Leu?Lys
80 85 90
aca?ctt?aaa?aaa?gcg?aaa?gta?aac?cgc?gaa?tac?gac?cgt?gta?ttt?tcg 821
Thr?Leu?Lys?Lys?Ala?Lys?Val?Asn?Arg?Glu?Tyr?Asp?Arg?Val?Phe?Ser
95 100 105
ggc?ttt?tct?atg?aaa?ctg?ccg?gcc?agt?gaa?att?cca?aag?ctg?ctc?gcc 869
Gly?Phe?Ser?Met?Lys?Leu?Pro?Ala?Ser?Glu?Ile?Pro?Lys?Leu?Leu?Ala
110 115 120
gtc?aag?gaa?gtt?aaa?gcg?gtt?tat?cca?aac?gca?act?tac?aaa?cct?gac 917
Val?Lys?Glu?Val?Lys?Ala?Val?Tyr?Pro?Asn?Ala?Thr?Tyr?Lys?Pro?Asp
125 130 135
agc?gta?aaa?gga?aaa?gac?gtg?aca?ctt?gca?gca?gac?gcc?att?tat?cca 965
Ser?Val?Lys?Gly?Lys?Asp?Val?Thr?Leu?Ala?Ala?Asp?Ala?Ile?Tyr?Pro
140 145 150 155
cag?atg?gat?aaa?agt?gcc?ccg?ttc?atc?gga?gcg?gat?cag?gca?tgg?aaa 1013
Gln?Met?Asp?Lys?Ser?Ala?Pro?Phe?Ile?Gly?Ala?Asp?Gln?Ala?Trp?Lys
160 165 170
tcg?ggc?tat?acc?ggt?aaa?ggc?att?aag?gta?gcc?gtt?att?gat?acg?ggc 1061
Ser?Gly?Tyr?Thr?Gly?Lys?Gly?Ile?Lys?Val?Ala?Val?Ile?Asp?Thr?Gly
175 180 185
gtg?gat?tac?acc?cat?ccc?gac?tta?aag?aat?aat?ttc?ggc?cca?tat?aag 1109
Val?Asp?Tyr?Thr?His?Pro?Asp?Leu?Lys?Asn?Asn?Phe?Gly?Pro?Tyr?Lys
190 195 200
ggc?tat?gac?ttt?gtg?gat?aat?gat?tat?gat?cca?cag?gaa?aca?ccg?aca 1157
Gly?Tyr?Asp?Phe?Val?Asp?Asn?Asp?Tyr?Asp?Pro?Gln?Glu?Thr?Pro?Thr
205 210 215
ggc?gac?ccg?cgc?ggc?gga?gcg?act?gat?cac?ggc?act?cat?gtt?gca?gga 1205
Gly?Asp?Pro?Arg?Gly?Gly?Ala?Thr?Asp?His?Gly?Thr?His?Val?Ala?Gly
220 225 230 235
aca?atc?gcc?gcg?aac?gga?cag?att?aaa?ggc?gtg?gcg?cct?gaa?gca?acg 1253
Thr?Ile?Ala?Ala?Asn?Gly?Gln?Ile?Lys?Gly?Val?Ala?Pro?Glu?Ala?Thr
240 245 250
ctt?ctt?gct?tac?cgc?gtg?ctc?ggc?ccc?ggc?ggc?tca?ggc?aca?acc?gag 1301
Leu?Leu?Ala?Tyr?Arg?Val?Leu?Gly?Pro?Gly?Gly?Ser?Gly?Thr?Thr?Glu
255 260 265
aat?gtg?att?gcg?ggc?att?gaa?aaa?gcc?gtt?gca?gac?gga?gca?aaa?gtg 1349
Asn?Val?Ile?Ala?Gly?Ile?Glu?Lys?Ala?Val?Ala?Asp?Gly?Ala?Lys?Val
270 275 280
atg?aac?ctc?tca?ttg?gga?aat?tcg?ctg?aac?agc?cct?gat?tat?gca?aca 1397
Met?Asn?Leu?Ser?Leu?Gly?Asn?Ser?Leu?Asn?Ser?Pro?Asp?Tyr?Ala?Thr
285 290 295
agc?atc?gca?ctg?gac?tgg?gcg?atg?gct?gaa?ggg?gtt?gtc?gcc?gtt?acg 1445
Ser?Ile?Ala?Leu?Asp?Trp?Ala?Met?Ala?Glu?Gly?Val?Val?Ala?Val?Thr
300 305 310 315
tca?aac?ggt?aac?agc?gga?ccg?gaa?aac?tgg?acg?gtc?ggc?tcg?ccg?gga 1493
Ser?Asn?Gly?Asn?Ser?Gly?Pro?Glu?Asn?Trp?Thr?Val?Gly?Ser?Pro?Gly
320 325 330
aca?tca?agg?gtt?gcg?att?tcc?gtc?ggc?gca?tca?cag?ctt?ccg?tat?aat 1541
Thr?Ser?Arg?Val?Ala?Ile?Ser?Val?Gly?Ala?Ser?Gln?Leu?Pro?Tyr?Asn
335 340 345
gag?tat?tcg?gtg?acg?ctt?cct?tcg?tat?tca?tca?gca?aag?gtg?atg?gga 1589
Glu?Tyr?Ser?Val?Thr?Leu?Pro?Ser?Tyr?Ser?Ser?Ala?Lys?Val?Met?Gly
350 355 360
tac?caa?gag?gaa?aag?gat?ctt?gaa?gca?ttg?aac?ggt?caa?gag?gtg?gaa 1637
Tyr?Gln?Glu?Glu?Lys?Asp?Leu?Glu?Ala?Leu?Asn?Gly?Gln?Glu?Val?Glu
365 370 375
ctc?gtt?gaa?gcc?ggc?ctc?gga?caa?gct?gac?gat?ttc?tca?ggc?aaa?gat 1685
Leu?Val?Glu?Ala?Gly?Leu?Gly?Gln?Ala?Asp?Asp?Phe?Ser?Gly?Lys?Asp
380 385 390 395
gtg?aaa?gga?aaa?gtt?gcg?gtt?atc?cag?aga?ggc?gtc?ata?cca?ttt?gtt 1733
Val?Lys?Gly?Lys?Val?Ala?Val?Ile?Gln?Arg?Gly?Val?Ile?Pro?Phe?Val
400 405 410
gat?aag?gct?gaa?aat?gcc?aaa?aac?gcc?ggc?gct?atc?gga?gcc?gtg?att 178l
Asp?Lys?Ala?Glu?Asn?Ala?Lys?Asn?Ala?Gly?Ala?Ile?Gly?Ala?Val?Ile
415 420 425
tac?aat?aat?gca?aca?ggg?gaa?atc?gag?gca?aac?gtc?atg?ggg?atg?gcc 1829
Tyr?Asn?Asn?Ala?Thr?Gly?Glu?Ile?Glu?Ala?Asn?Val?Met?Gly?Met?Ala
430 435 440
gta?ccg?acc?gtc?aaa?ttg?tca?aaa?gaa?gag?ggc?gaa?aag?ctt?gtt?caa 1877
Val?Pro?Thr?Val?Lys?Leu?Ser?Lys?Glu?Glu?Gly?Glu?Lys?Leu?Val?Gln
445 450 455
cag?atc?aaa?gaa?ggg?aaa?cac?tcc?gtt?gtc?ttt?tct?ttc?aaa?ttg?gac 1925
Gln?Ile?Lys?Glu?Gly?Lys?His?Ser?Val?Val?Phe?Ser?Phe?Lys?Leu?Asp
460 465 470 475
aaa?aag?ctg?ggg?gaa?aca?att?gcc?tca?ttc?tcg?tcc?cgc?ggg?cct?gtc 1973
Lys?Lys?Leu?Gly?Glu?Thr?Ile?Ala?Ser?Phe?Ser?Ser?Arg?Gly?Pro?Val
480 485 490
atg?gat?aca?tgg?atg?att?aaa?cct?gac?gtt?tca?gcg?cca?ggc?gtc?aac 2021
Met?Asp?Thr?Trp?Met?Ile?Lys?Pro?Asp?Val?Ser?Ala?Pro?Gly?Val?Asn
495 500 505
atc?gtc?agc?acc?att?ccg?acc?cac?gat?ccg?aaa?aac?ccg?tac?ggt?tac 2069
Ile?Val?Ser?Thr?Ile?Pro?Thr?His?Asp?Pro?Lys?Asn?Pro?Tyr?Gly?Tyr
510 515 520
ggt?tca?aaa?cag?gga?aca?agc?atg?gct?tcc?ccg?cat?gtc?gcg?gga?acg 2117
Gly?Ser?Lys?Gln?Gly?Thr?Ser?Met?Ala?Ser?Pro?His?Val?Ala?Gly?Thr
525 530 535
gca?gcc?ata?tta?aag?cag?gcc?aaa?ccg?gat?tgg?acg?cct?gag?caa?atc 2165
Ala?Ala?Ile?Leu?Lys?Gln?Ala?Lys?Pro?Asp?Trp?Thr?Pro?Glu?Gln?Ile
540 545 550 555
aaa?ggc?gta?ctg?atg?aat?acg?gcg?gaa?aaa?ctg?acg?gat?gaa?aat?gga 2213
Lys?Gly?Val?Leu?Met?Asn?Thr?Ala?Glu?Lys?Leu?Thr?Asp?Glu?Asn?Gly
560 565 570
aag?cct?ctc?cct?cac?aat?acg?caa?gga?gcg?ggc?agc?atc?cgc?atc?atg 2261
Lys?Pro?Leu?Pro?His?Asn?Thr?Gln?Gly?Ala?Gly?Ser?Ile?Arg?Ile?Met
575 580 585
gag?gcc?ctt?aaa?gct?tca?tcc?att?gta?acg?ccg?ggc?agc?cat?tca?tac 2309
Glu?Ala?Leu?Lys?Ala?Ser?Ser?Ile?Val?Thr?Pro?Gly?Ser?His?Ser?Tyr
590 595 600
gga?aca?ttc?ctg?aaa?gac?aaa?ggg?aaa?cag?acg?aaa?aaa?caa?gcg?ttc 2357
Gly?Thr?Phe?Leu?Lys?Asp?Lys?Gly?Lys?Gln?Thr?Lys?Lys?Gln?Ala?Phe
605 610 615
acg?att?gaa?aac?ctt?tct?tca?cac?aga?aaa?gcc?tat?cag?ctc?gaa?tac 2405
Thr?Ile?Glu?Asn?Leu?Ser?Ser?His?Arg?Lys?Ala?Tyr?Gln?Leu?Glu?Tyr
620 625 630 635
tcc?ttt?aaa?gga?acg?ggc?atc?acg?gta?tca?gga?acg?gaa?cga?gtc?gtg 2453
Ser?Phe?Lys?Gly?Thr?Gly?Ile?Thr?Val?Ser?Gly?Thr?Glu?Arg?Val?Val
640 645 650
gta?ccg?gcc?aat?caa?aca?ggt?aaa?gca?gcg?gca?aaa?gta?acc?gtc?aat 2501
Val?Pro?Ala?Asn?Gln?Thr?Gly?Lys?Ala?Ala?Ala?Lys?Val?Thr?Val?Asn
655 660 665
tcc?gcg?aaa?acg?aaa?gca?ggc?aca?tat?gaa?ggc?acg?gtt?tac?atc?cgt 2549
Ser?Ala?Lys?Thr?Lys?Ala?Gly?Thr?Tyr?Glu?Gly?Thr?Val?Tyr?Ile?Arg
670 675 680
gaa?gac?gga?aga?aaa?gtc?gcc?gaa?atc?ccg?ctc?cta?ttg?atc?gtc?aaa 2597
Glu?Asp?Gly?Arg?Lys?Val?Ala?Glu?Ile?Pro?Leu?Leu?Leu?Ile?Val?Lys
685 690 695
gag?cca?gac?tac?ccg?cgc?gtc?aca?tcc?gta?aca?gtt?gaa?ccg?gga?gca 2645
Glu?Pro?Asp?Tyr?Pro?Arg?Val?Thr?Ser?Val?Thr?Val?Glu?Pro?Gly?Ala
700 705 710 715
aag?cag?ggc?gct?tac?acg?atc?gaa?gcc?tac?ctg?ccg?ggc?ggg?gct?gaa 2693
Lys?Gln?Gly?Ala?Tyr?Thr?Ile?Glu?Ala?Tyr?Leu?Pro?Gly?Gly?Ala?Glu
720 725 730
gag?ctc?gca?ttt?ctc?gtc?tat?gat?gaa?aac?ctg?aac?ctt?ctc?ggc?cag 2741
Glu?Leu?Ala?Phe?Leu?Val?Tyr?Asp?Glu?Asn?Leu?Asn?Leu?Leu?Gly?Gln
735 740 745
gcc?ggc?gtt?tac?aaa?aac?cag?ggc?aaa?ggc?tat?caa?tct?tat?caa?tgg 2789
Ala?Gly?Val?Tyr?Lys?Asn?Gln?Gly?Lys?Gly?Tyr?Gln?Ser?Tyr?Gln?Trp
750 755 760
aac?ggc?aaa?atc?aat?gac?gcc?gca?tcc?ctt?aag?tcc?gga?aaa?tac?tat 2837
Asn?Gly?Lys?Ile?Asn?Asp?Ala?Ala?Ser?Leu?Lys?Ser?Gly?Lys?Tyr?Tyr
765 770 775
atg?ctt?gcc?tat?gca?tcc?gcc?aaa?ggg?aaa?tca?agc?tac?gta?ttg?acg 2885
Met?Leu?Ala?Tyr?Ala?Ser?Ala?Lys?Gly?Lys?Ser?Ser?Tyr?Val?Leu?Thr
780 785 790 795
gaa?gac?cct?ttt?atc?gtc?gaa?taatgacaag?ccttgttgag?aaccactcaa 2936
Glu?Asp?Pro?Phe?Ile?Val?Glu
800
caaggctttt?ttatgttaaa?atacggataa?tgcgttcagg?agaagctccc?ccttctcttc 2996
aaaacgtgaa?aaaagcaatc?ggaggacatc?gtgtatatgc?tttcttttat?cgtattattc 3056
ggcttatcct?tcattattgt?ctgctttata?tttttcacga?ttttgtactt?cgccgtcaac 3116
ctgcagaagc?gcgagcccaa?gccttttcaa?aaagctgcgg?agcaaaccgt?cgataccatc 3176
atcctcattc?cgatcagctg?gctgtttacc?gctttataca?tatgcattct?gtttattctt 3236
ttcccaatcc?gccattttct?cgattttttt?cagcaaaaac?gctaaattga?ctgatgaaac 3296
gcttcggcca?gcagccggta?tgaatccaat?ctgtcttgaa?aatcgtgggt?gatcgtcacc 3356
gccatgattt?cgtccgttcc?gtaagcgccg?gccagttcaa?gcagctgtt 3405
<210>4
<211>802
<212>PRT
＜213〉Bacillus licheniformis
<400>4
Leu?Arg?Lys?Ser?Ile?Val?Arg?Tyr?Phe?Val?Met?Ala?Phe?Ile?Leu?Leu
1 5 10 15
Phe?Ala?Leu?Ser?Thr?Phe?Leu?Thr?Gly?Val?Gln?Ala?Thr?Ser?Val?Pro
20 25 30
Asp?Lys?Lys?Ser?Pro?Glu?Leu?Glu?Lys?Ala?Glu?Ile?Tyr?Gly?Asp?Ile
35 40 45
Asp?Val?Thr?Ser?Asp?Lys?Gln?Thr?Thr?Val?Ile?Val?Glu?Leu?Lys?Glu
50 55 60
Lys?Ser?Leu?Ala?Glu?Ala?Lys?Ala?Asp?Gly?Glu?Lys?Gln?Thr?Lys?Ala
65 70 75 80
Ser?Leu?Lys?Thr?Ala?Arg?Ser?Lys?Ala?Leu?Lys?Thr?Leu?Lys?Lys?Ala
85 90 95
Lys?Val?Asn?Arg?Glu?Tyr?Asp?Arg?Val?Phe?Ser?Gly?Phe?Ser?Met?Lys
100 105 110
Leu?Pro?Ala?Ser?Glu?Ile?Pro?Lys?Leu?Leu?Ala?Val?Lys?Glu?Val?Lys
115 120 125
Ala?Val?Tyr?Pro?Asn?Ala?Thr?Tyr?Lys?Pro?Asp?Ser?Val?Lys?Gly?Lys
130 135 140
Asp?Val?Thr?Leu?Ala?Ala?Asp?Ala?Ile?Tyr?Pro?Gln?Met?Asp?Lys?Ser
145 150 155 160
Ala?Pro?Phe?Ile?Gly?Ala?Asp?Gln?Ala?Trp?Lys?Ser?Gly?Tyr?Thr?Gly
165 170 175
Lys?Gly?Ile?Lys?Val?Ala?Val?Ile?Asp?Thr?Gly?Val?Asp?Tyr?Thr?His
180 185 190
Pro?Asp?Leu?Lys?Asn?Asn?Phe?Gly?Pro?Tyr?Lys?Gly?Tyr?Asp?Phe?Val
195 200 205
Asp?Asn?Asp?Tyr?Asp?Pro?Gln?Glu?Thr?Pro?Thr?Gly?Asp?Pro?Arg?Gly
210 215 220
Gly?Ala?Thr?Asp?His?Gly?Thr?His?Val?Ala?Gly?Thr?Ile?Ala?Ala?Asn
225 230 235 240
Gly?Gln?Ile?Lys?Gly?Val?Ala?Pro?Glu?Ala?Thr?Leu?Leu?Ala?Tyr?Arg
245 250 255
Val?Leu?Gly?Pro?Gly?Gly?Ser?Gly?Thr?Thr?Glu?Asn?Val?Ile?Ala?Gly
260 265 270
Ile?Glu?Lys?Ala?Val?Ala?Asp?Gly?Ala?Lys?Val?Met?Asn?Leu?Ser?Leu
275 280 285
Gly?Asn?Ser?Leu?Asn?Ser?Pro?Asp?Tyr?Ala?Thr?Ser?Ile?Ala?Leu?Asp
290 295 300
Trp?Ala?Met?Ala?Glu?Gly?Val?Val?Ala?Val?Thr?Ser?Asn?Gly?Asn?Ser
305 310 315 320
Gly?Pro?Glu?Asn?Trp?Thr?Val?Gly?Ser?Pro?Gly?Thr?Ser?Arg?Val?Ala
325 330 335
Il?e?Ser?Val?Gly?Ala?Ser?Gln?Leu?Pro?Tyr?Asn?Glu?Tyr?Ser?Val?Thr
340 345 350
Leu?Pro?Ser?Tyr?Ser?Ser?Ala?Lys?Val?Met?Gly?Tyr?Gln?Glu?Glu?Lys
355 360 365
Asp?Leu?Glu?Ala?Leu?Asn?Gly?Gln?Glu?Val?Glu?Leu?Val?Glu?Ala?Gly
370 375 380
Leu?Gly?Gln?Ala?Asp?Asp?Phe?Ser?Gly?Lys?Asp?Val?Lys?Gly?Lys?Val
385 390 395 400
Ala?Val?Ile?Gln?Arg?Gly?Val?Ile?Pro?Phe?Val?Asp?Lys?Ala?Glu?Asn
405 410 415
Ala?Lys?Asn?Ala?Gly?Ala?Ile?Gly?Ala?Val?Ile?Tyr?Asn?Asn?Ala?Thr
420 425 430
Gly?Glu?Ile?Glu?Ala?Asn?Val?Met?Gly?Met?Ala?Val?Pro?Thr?Val?Lys
435 440 445
Leu?Ser?Lys?Glu?Glu?Gly?Glu?Lys?Leu?Val?Gln?Gln?Ile?Lys?Glu?Gly
450 455 460
Lys?His?Ser?Val?Val?Phe?Ser?Phe?Lys?Leu?Asp?Lys?Lys?Leu?Gly?Glu
465 470 475 480
Thr?Ile?Ala?Ser?Phe?Ser?Ser?Arg?Gly?Pro?Val?Met?Asp?Thr?Trp?Met
485 490 495
Ile?Lys?Pro?Asp?Val?Ser?Ala?Pro?Gly?Val?Asn?Ile?Val?Ser?Thr?Ile
500 505 510
Pro?Thr?His?Asp?Pro?Lys?Asn?Pro?Tyr?Gly?Tyr?Gly?Ser?Lys?Gln?Gly
515 520 525
Thr?Ser?Met?Ala?Ser?Pro?His?Val?Ala?Gly?Thr?Ala?Ala?Ile?Leu?Lys
530 535 540
Gln?Ala?Lys?Pro?Asp?Trp?Thr?Pro?Glu?Gln?Ile?Lys?Gly?Val?Leu?Met
545 550 555 560
Asn?Thr?Ala?Glu?Lys?Leu?Thr?Asp?Glu?Asn?Gly?Lys?Pro?Leu?Pro?His
565 570 575
Asn?Thr?Gln?Gly?Ala?Gly?Ser?Ile?Arg?Ile?Met?Glu?Ala?Leu?Lys?Ala
580 585 590
Ser?Ser?Ile?Val?Thr?Pro?Gly?Ser?His?Ser?Tyr?Gly?Thr?Phe?Leu?Lys
595 600 605
Asp?Lys?Gly?Lys?Gln?Thr?Lys?Lys?Gln?Ala?Phe?Thr?Ile?Glu?Asn?Leu
610 615 620
Ser?Ser?His?Arg?Lys?Ala?Tyr?Gln?Leu?Glu?Tyr?Ser?Phe?Lys?Gly?Thr
625 630 635 640
Gly?Ile?Thr?Val?Ser?Gly?Thr?Glu?Arg?Val?Val?Val?Pro?Ala?Asn?Gln
645 650 655
Thr?Gly?Lys?Ala?Ala?Ala?Lys?Val?Thr?Val?Asn?Ser?Ala?Lys?Thr?Lys
660 665 670
Ala?Gly?Thr?Tyr?Glu?Gly?Thr?Val?Tyr?Ile?Arg?Glu?Asp?Gly?Arg?Lys
675 680 685
Val?Ala?Glu?Ile?Pro?Leu?Leu?Leu?Ile?Val?Lys?Glu?Pro?Asp?Tyr?Pro
690 695 700
Arg?Val?Thr?Ser?Val?Thr?Val?Glu?Pro?Gly?Ala?Lys?Gln?Gly?Ala?Tyr
705 710 715 720
Thr?Ile?Glu?Ala?Tyr?Leu?Pro?Gly?Gly?Ala?Glu?Glu?Leu?Ala?Phe?Leu
725 730 735
Val?Tyr?Asp?Glu?Asn?Leu?Asn?Leu?Leu?Gly?Gln?Ala?Gly?Val?Tyr?Lys
740 745 750
Asn?Gln?Gly?Lys?Gly?Tyr?Gln?Ser?Tyr?Gln?Trp?Asn?Gly?Lys?Ile?Asn
755 760 765
Asp?Ala?Ala?Ser?Leu?Lys?Ser?Gly?Lys?Tyr?Tyr?Met?Leu?Ala?Tyr?Ala
770 775 780
Ser?Ala?Lys?Gly?Lys?Ser?Ser?Tyr?Val?Leu?Thr?Glu?Asp?Pro?Phe?Ile
785 790 795 800
Val?Glu
<210>5
<211>2771
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(175)..(2268)
<400>5
gggcctactg?tccttaaaaa?attacacttc?tatccagaac?aaaagaaaaa?gctagaactt 60
gaaagtcaaa?gaaggtcaat?gtatactaat?agteagaaat?ggtcaaactg?cagaccattt 120
tttctaagtc?aattggtcaa?aaaaggtcaa?attcaataaa?gggggttttg?gcaa?atg 177
Met
1
cgt?tgt?caa?cat?tgt?caa?aaa?aat?gag?gcg?acg?att?cgc?cta?aac?atg 225
Arg?Cys?Gln?His?Cys?Gln?Lys?Asn?Glu?Ala?Thr?Ile?Arg?Leu?Asn?Met
5 10 15
caa?atc?aac?ttt?aca?aaa?aaa?caa?atg?gat?tta?tgt?gaa?gac?tgc?tat 273
Gln?Ile?Asn?Phe?Thr?Lys?Lys?Gln?Met?Asp?Leu?Cys?Glu?Asp?Cys?Tyr
20 25 30
aaa?gag?ctg?act?cag?cct?tct?atg?ttt?tca?gga?aat?cac?ttt?ttt?ggc 321
Lys?Glu?Leu?Thr?Gln?Pro?Ser?Met?Phe?Ser?Gly?Asn?His?Phe?Phe?Gly
35 40 45
ggc?gga?ggt?tct?ttc?ttc?agc?cag?cca?tca?caa?gcg?cag?acg?caa?acc 369
Gly?Gly?Gly?Ser?Phe?Phe?Ser?Gln?Pro?Ser?Gln?Ala?Gln?Thr?Gln?Thr
50 55 60 65
gct?gcc?aaa?aaa?ggc?ttg?ctc?gat?gaa?ctt?gcc?gtc?aat?tta?aca?aat 417
Ala?Ala?Lys?Lys?Gly?Leu?Leu?Asp?Glu?Leu?Ala?Val?Asn?Leu?Thr?Asn
70 75 80
gca?gca?aag?acc?ggc?ttg?atc?gat?ccg?gtg?atc?ggc?cgc?gat?gat?gaa 465
Ala?Ala?Lys?Thr?Gly?Leu?Ile?Asp?Pro?Val?Ile?Gly?Arg?Asp?Asp?Glu
85 90 95
gta?gcc?cgt?gtg?atc?gag?atc?tta?aac?cgc?aga?aat?aaa?aac?aat?ccg 513
Val?Ala?Arg?Val?Ile?Glu?Ile?Leu?Asn?Arg?Arg?Asn?Lys?Asn?Asn?Pro
100 105 110
gtc?tta?atc?ggt?gaa?ccg?ggc?gtc?ggt?aaa?aca?gcc?gtc?gct?gaa?gga 561
Val?Leu?Ile?Gly?Glu?Pro?Gly?Val?Gly?Lys?Thr?Ala?Val?Ala?Glu?Gly
115 120 125
ctt?gct?ctc?aaa?atc?gcc?gaa?ggc?gat?gtt?cca?aat?aaa?ttg?aaa?aac 609
Leu?Ala?Leu?Lys?Ile?Ala?Glu?Gly?Asp?Val?Pro?Asn?Lys?Leu?Lys?Asn
130 135 140 145
aaa?gag?ctc?tac?ctg?ctt?gat?gtc?gct?tcc?ctc?gtg?gca?aac?acc?ggc 657
Lys?Glu?Leu?Tyr?Leu?Leu?Asp?Val?Ala?Ser?Leu?Val?Ala?Asn?Thr?Gly
150 155 160
atc?aga?ggg?caa?ttt?gaa?gaa?aga?atg?aaa?cag?ctg?atg?aaa?gag?ctg 705
Ile?Arg?Gly?Gln?Phe?Glu?Glu?Arg?Met?Lys?Gln?Leu?Met?Lys?Glu?Leu
165 170 175
aaa?gaa?cgc?aaa?aac?gca?atc?ttg?ttt?att?gac?gaa?att?cac?ctt?ctt 753
Lys?Glu?Arg?Lys?Asn?Ala?Ile?Leu?Phe?Ile?Asp?Glu?Ile?His?Leu?Leu
180 185 190
gtc?ggg?gcc?ggc?tct?gca?gaa?ggc?tcc?atg?gat?gcg?ggc?aac?att?tta 801
Val?Gly?Ala?Gly?Ser?Ala?Glu?Gly?Ser?Met?Asp?Ala?Gly?Asn?Ile?Leu
195 200 205
aaa?cct?gca?ctt?gcc?aga?ggc?gag?ctg?caa?gtt?atc?ggc?gct?acg?acg 849
Lys?Pro?Ala?Leu?Ala?Arg?Gly?Glu?Leu?Gln?Val?Ile?Gly?Ala?Thr?Thr
210 215 220 225
ttg?aag?gaa?tat?cgc?caa?att?gaa?aaa?gat?gcg?gcg?tta?gag?cgg?cgc 897
Leu?Lys?Glu?Tyr?Arg?Gln?Ile?Glu?Lys?Asp?Ala?Ala?Leu?Glu?Arg?Arg
230 235 240
ttt?cag?ccg?atc?atg?gtg?cac?gaa?ccg?aca?att?gag?cag?gcc?gca?gcg 945
Phe?Gln?Pro?Ile?Met?Val?His?Glu?Pro?Thr?Ile?Glu?Gln?Ala?Ala?Ala
245 250 255
att?tta?aac?ggg?ctc?aag?gaa?aaa?tac?gaa?gca?tat?cac?gat?gtg?act 993
Ile?Leu?Asn?Gly?Leu?Lys?Glu?Lys?Tyr?Glu?Ala?Tyr?His?Asp?Val?Thr
260 265 270
tac?acg?gat?gaa?gcg?atc?aaa?gcc?tgc?gtc?att?ctg?tca?agc?cgc?tat 1041
Tyr?Thr?Asp?Glu?Ala?Ile?Lys?Ala?Cys?Val?Ile?Leu?Ser?Ser?Arg?Tyr
275 280 285
att?cag?gac?cgc?cat?ctt?ccc?gat?aag?gcg?atc?gac?ctt?ttg?gat?gaa 1089
Ile?Gln?Asp?Arg?His?Leu?Pro?Asp?Lys?Ala?Ile?Asp?Leu?Leu?Asp?Glu
290 295 300 305
gcc?ggt?tca?aaa?gcg?aac?ttg?aaa?atc?gac?acg?gtc?agc?ggt?gaa?caa 1137
Ala?Gly?Ser?Lys?Ala?Asn?Leu?Lys?Ile?Asp?Thr?Val?Ser?Gly?Glu?Gln
310 315 320
gct?tcc?caa?cgc?ctg?caa?gaa?atc?gaa?gcg?gaa?aaa?gcc?aaa?gcg?ctt 1185
Ala?Ser?Gln?Arg?Leu?Gln?Glu?Ile?Glu?Ala?Glu?Lys?Ala?Lys?Ala?Leu
325 330 335
caa?gaa?gaa?aac?tac?gag?ctt?gca?gca?aaa?ctc?cgc?gac?gaa?gaa?gaa 1233
Gln?Glu?Glu?Asn?Tyr?Glu?Leu?Ala?Ala?Lys?Leu?Arg?Asp?Glu?Glu?Glu
340 345 350
gcg?ctg?cat?aaa?aaa?atg?gaa?agc?cat?gag?gaa?gaa?aaa?cgg?gca?aca 128l
Ala?Leu?His?Lys?Lys?Met?Glu?Ser?His?Glu?Glu?Glu?Lys?Arg?Ala?Thr
355 360 365
gtt?gaa?gcg?gaa?gac?att?caa?gcg?atc?atc?gaa?caa?aaa?acc?ggc?att 1329
Val?Glu?Ala?Glu?Asp?Ile?Gln?Ala?Ile?Ile?Glu?Gln?Lys?Thr?Gly?Ile
370 375 380 385
ccg?gtc?ggc?aag?ctt?cag?cac?gac?gat?cag?aag?aaa?atg?aaa?gag?ctt 1377
Pro?Val?Gly?Lys?Leu?Gln?His?Asp?Asp?Gln?Lys?Lys?Met?Lys?Glu?Leu
390 395 400
gaa?gca?cac?ctt?tca?agc?cgc?gtg?atc?ggt?caa?aaa?gaa?gcg?gtg?aaa 1425
Glu?Ala?His?Leu?Ser?Ser?Arg?Val?Ile?Gly?Gln?Lys?Glu?Ala?Val?Lys
405 410 415
aaa?gtg?gca?aaa?gcc?gtc?aga?aga?agc?cgc?gcc?ggc?ctg?aaa?tca?aaa 1473
Lys?Val?Ala?Lys?Ala?Val?Arg?Arg?Ser?Arg?Ala?Gly?Leu?Lys?Ser?Lys
420 425 430
aac?agg?cct?gtc?ggt?tca?ttc?ctg?ttc?gtc?ggc?ccg?aca?ggt?gtc?ggt 1521
Asn?Arg?Pro?Val?Gly?Ser?Phe?Leu?Phe?Val?Gly?Pro?Thr?Gly?Val?Gly
435 440 445
aaa?aca?gaa?ctt?tct?aaa?agg?ctt?gcg?gaa?gaa?ctg?ttc?ggc?acg?aag 1569
Lys?Thr?Glu?Leu?Ser?Lys?Arg?Leu?Ala?Glu?Glu?Leu?Phe?Gly?Thr?Lys
450 455 460 465
gat?gcc?atc?atc?cgc?ctc?gat?atg?agc?gaa?tac?atg?gaa?aaa?cac?gcc 1617
Asp?Ala?Ile?Ile?Arg?Leu?Asp?Met?Ser?Glu?Tyr?Met?Glu?Lys?His?Ala
470 475 480
gta?tca?aag?ctg?atc?ggt?tcg?cct?cct?gga?tat?gtc?ggc?cac?gaa?gaa 1665
Val?Ser?Lys?Leu?Ile?Gly?Ser?Pro?Pro?Gly?Tyr?Val?Gly?His?Glu?Glu
485 490 495
gcc?gga?cag?ctt?acc?gaa?aaa?gtc?cgg?agg?aac?ccg?tac?agc?atc?gtt 1713
Ala?Gly?Gln?Leu?Thr?Glu?Lys?Val?Arg?Arg?Asn?Pro?Tyr?Ser?Ile?Val
500 505 510
ttg?ctc?gat?gaa?atc?gaa?aaa?gcc?cat?ccc?gat?gtc?cag?cac?atg?ttc 1761
Leu?Leu?Asp?Glu?Ile?Glu?Lys?Ala?His?Pro?Asp?Val?Gln?His?Met?Phe
515 520 525
ctg?caa?atc?atg?gaa?gac?gga?cgt?ctg?aca?gac?agc?caa?ggg?cgg?acg 1809
Leu?Gln?Ile?Met?Glu?Asp?Gly?Arg?Leu?Thr?Asp?Ser?Gln?Gly?Arg?Thr
530 535 540 545
gtc?agc?ttt?aaa?gat?acc?gtc?att?att?atg?aca?agc?aat?gcc?ggt?gta 1857
Val?Ser?Phe?Lys?Asp?Thr?Val?Ile?Ile?Met?Thr?Ser?Asn?Ala?Gly?Val
550 555 560
tct?gaa?aag?cgg?aca?acc?gtc?ggt?ttt?aac?cgg?gaa?gag?caa?gta?atg 1905
Ser?Glu?Lys?Arg?Thr?Thr?Val?Gly?Phe?Asn?Arg?Glu?Glu?Gln?Val?Met
565 570 575
ggc?gaa?caa?tca?atg?atc?gat?tca?tta?agc?ggc?tac?ttt?aag?ccg?gaa 1953
Gly?Glu?Gln?Ser?Met?Ile?Asp?Ser?Leu?Ser?Gly?Tyr?Phe?Lys?Pro?Glu
580 585 590
ttc?ctg?aac?aga?ttt?gac?agc?atc?atc?gaa?ttc?cag?ccg?ctc?gaa?aaa 2001
Phe?Leu?Asn?Arg?Phe?Asp?Ser?Ile?Ile?Glu?Phe?Gln?Pro?Leu?Glu?Lys
595 600 605
gaa?gat?ctg?gtc?aag?atc?gcc?gcc?ctc?ttg?ctg?aaa?gag?ctt?gaa?gaa 2049
Glu?Asp?Leu?Val?Lys?Ile?Ala?Ala?Leu?Leu?Leu?Lys?Glu?Leu?Glu?Glu
610 615 620 625
acg?tta?aaa?gag?caa?aac?atg?acg?ctc?gaa?gtt?tca?gat?gag?gca?aaa 2097
Thr?Leu?Lys?Glu?Gln?Asn?Met?Thr?Leu?Glu?Val?Ser?Asp?Glu?Ala?Lys
630 635 640
gaa?aaa?atc?gct?gaa?atc?gga?tac?cat?ccg?gcc?ttt?ggc?gcc?cgc?cca 2145
Glu?Lys?Ile?Ala?Glu?Ile?Gly?Tyr?His?Pro?Ala?Phe?Gly?Ala?Arg?Pro
645 650 655
tta?aga?aga?acc?att?caa?aca?gcg?gtc?gaa?gat?caa?atg?acc?gat?ctg 2193
Leu?Arg?Arg?Thr?Ile?Gln?Thr?Ala?Val?Glu?Asp?Gln?Met?Thr?Asp?Leu
660 665 670
ctg?ctt?gaa?gaa?gaa?cac?atc?aaa?ggt?ttt?gcg?gtt?atc?gtt?gaa?aac 2241
Leu?Leu?Glu?Glu?Glu?His?Ile?Lys?Gly?Phe?Ala?Val?Ile?Val?Glu?Asn
675 680 685
gat?gaa?atc?cgg?gtg?aaa?aca?acc?aga?taacacacac?aacggcctcc 2288
Asp?Glu?Ile?Arg?Val?Lys?Thr?Thr?Arg
690 695
gatcgcggag?gtcgttttac?atacaacata?agaatcacaa?aagttctatt?ttttaacatt 2348
aaataaccct?aaagtccctt?ttcaccatga?ttttgcaaca?atttaagaaa?cagcctaaag 2408
ttcctatcac?gcaacaccga?tattaacgat?agaaaagtta?gttaaaggaa?ttggtgaaaa 2468
aatggacaaa?acatcgttaa?ttggaatcat?cttagctatc?atcggagtag?gtgtcggcat 2528
ggtcatgaaa?ggtgtcagcc?cttccgttct?cgtcaatccc?gcggcatggc?tgatcatcct 2588
tgtcgggaca?atcgcagcag?ttgtgatcgc?ttttccaatc?cgcgagctca?aaaaggttcc 2648
caaactcttc?ggcatcttgt?tcaaagagaa?gaagatgatc?gaaatcaacg?aactgatccc 2708
gatgttttcc?gagtgggcgc?aaatcgcaag?acgcgaagga?cttctcgctt?tggaagcaaa 2768
ttt 2771
<210>6
<211>698
<212>PRT
＜213〉Bacillus licheniformis
<400>6
Met?Arg?Cys?Gln?His?Cys?Gln?Lys?Asn?Glu?Ala?Thr?Ile?Arg?Leu?Asn
1 5 10 15
Met?Gln?Ile?Asn?Phe?Thr?Lys?Lys?Gln?Met?Asp?Leu?Cys?Glu?Asp?Cys
20 25 30
Tyr?Lys?Glu?Leu?Thr?Gln?Pro?Ser?Met?Phe?Ser?Gly?Asn?His?Phe?Phe
35 40 45
Gly?Gly?Gly?Gly?Ser?Phe?Phe?Ser?Gln?Pro?Ser?Gln?Ala?Gln?Thr?Gln
50 55 60
Thr?Ala?Ala?Lys?Lys?Gly?Leu?Leu?Asp?Glu?Leu?Ala?Val?Asn?Leu?Thr
65 70 75 80
Asn?Ala?Ala?Lys?Thr?Gly?Leu?Ile?Asp?Pro?Val?Ile?Gly?Arg?Asp?Asp
85 90 95
Glu?Val?Ala?Arg?Val?Ile?Glu?Ile?Leu?Asn?Arg?Arg?Asn?Lys?Asn?Asn
100 105 110
Pro?Val?Leu?Ile?Gly?Glu?Pro?Gly?Val?Gly?Lys?Thr?Ala?Val?Ala?Glu
115 120 125
Gly?Leu?Ala?Leu?Lys?Ile?Ala?Glu?Gly?Asp?Val?Pro?Asn?Lys?Leu?Lys
130 135 140
Asn?Lys?Glu?Leu?Tyr?Leu?Leu?Asp?Val?Ala?Ser?Leu?Val?Ala?Asn?Thr
145 150 155 160
Gly?Ile?Arg?Gly?Gln?Phe?Glu?Glu?Arg?Met?Lys?Gln?Leu?Met?Lys?Glu
165 170 175
Leu?Lys?Glu?Arg?Lys?Asn?Ala?Ile?Leu?Phe?Ile?Asp?Glu?Ile?His?Leu
180 185 190
Leu?Val?Gly?Ala?Gly?Ser?Ala?Glu?Gly?Ser?Met?Asp?Ala?Gly?Asn?Ile
195 200 205
Leu?Lys?Pro?Ala?Leu?Ala?Arg?Gly?Glu?Leu?Gln?Val?Ile?Gly?Ala?Thr
2l0 215 220
Thr?Leu?Lys?Glu?Tyr?Arg?Gln?Ile?Glu?Lys?Asp?Ala?Ala?Leu?Glu?Arg
225 230 235 240
Arg?Phe?Gln?Pro?Ile?Met?Val?His?Glu?Pro?Thr?Ile?Glu?Gln?Ala?Ala
245 250 255
Ala?Ile?Leu?Asn?Gly?Leu?Lys?Glu?Lys?Tyr?Glu?Ala?Tyr?His?Asp?Val
260 265 270
Thr?Tyr?Thr?Asp?Glu?Ala?Ile?Lys?Ala?Cys?Val?Ile?Leu?Ser?Ser?Arg
275 280 285
Tyr?Ile?Gln?Asp?Arg?His?Leu?Pro?Asp?Lys?Ala?Ile?Asp?Leu?Leu?Asp
290 295 300
Glu?Ala?Gly?Ser?Lys?Ala?Asn?Leu?Lys?Ile?Asp?Thr?Val?Ser?Gly?Glu
305 310 315 320
Gln?Ala?Ser?Gln?Arg?Leu?Gln?Glu?Ile?Glu?Ala?Glu?Lys?Ala?Lys?Ala
325 330 335
Leu?Gln?Glu?Glu?Asn?Tyr?Glu?Leu?Ala?Ala?Lys?Leu?Arg?Asp?Glu?Glu
340 345 350
Glu?Ala?Leu?His?Lys?Lys?Met?Glu?Ser?His?Glu?Glu?Glu?Lys?Arg?Ala
355 360 365
Thr?Val?Glu?Ala?Glu?Asp?Ile?Gln?Ala?Ile?Ile?Glu?Gln?Lys?Thr?Gly
370 375 380
Ile?Pro?Val?Gly?Lys?Leu?Gln?His?Asp?Asp?Gln?Lys?Lys?Met?Lys?Glu
385 390 395 400
Leu?Glu?Ala?His?Leu?Ser?Ser?Arg?Val?Ile?Gly?Gln?Lys?Glu?Ala?Val
405 410 415
Lys?Lys?Val?Ala?Lys?Ala?Val?Arg?Arg?Ser?Arg?Ala?Gly?Leu?Lys?Ser
420 425 430
Lys?Asn?Arg?Pro?Val?Gly?Ser?Phe?Leu?Phe?Val?Gly?Pro?Thr?Gly?Val
435 440 445
Gly?Lys?Thr?Glu?Leu?Ser?Lys?Arg?Leu?Ala?Glu?Glu?Leu?Phe?Gly?Thr
450 455 460
Lys?Asp?Ala?Ile?Ile?Arg?Leu?Asp?Met?Ser?Glu?Tyr?Met?Glu?Lys?His
465 470 475 480
Ala?Val?Ser?Lys?Leu?Ile?Gly?Ser?Pro?Pro?Gly?Tyr?Val?Gly?His?Glu
485 490 495
Glu?Ala?Gly?Gln?Leu?Thr?Glu?Lys?Val?Arg?Arg?Asn?Pro?Tyr?Ser?Ile
500 505 510
Val?Leu?Leu?Asp?Glu?Ile?Glu?Lys?Ala?His?Pro?Asp?Val?Gln?His?Met
515 520 525
Phe?Leu?Gln?Ile?Met?Glu?Asp?Gly?Arg?Leu?Thr?Asp?Ser?Gln?Gly?Arg
530 535 540
Thr?Val?Ser?Phe?Lys?Asp?Thr?Val?Ile?Ile?Met?Thr?Ser?Asn?Ala?Gly
545 550 555 560
Val?Ser?Glu?Lys?Arg?Thr?Thr?Val?Gly?Phe?Asn?Arg?Glu?Glu?Gln?Val
565 570 575
Met?Gly?Glu?Gln?Ser?Met?Ile?Asp?Ser?Leu?Ser?Gly?Tyr?Phe?Lys?Pro
580 585 590
Glu?Phe?Leu?Asn?Arg?Phe?Asp?Ser?Ile?Ile?Glu?Phe?Gln?Pro?Leu?Glu
595 600 605
Lys?Glu?Asp?Leu?Val?Lys?Ile?Ala?Ala?Leu?Leu?Leu?Lys?Glu?Leu?Glu
610 615 620
Glu?Thr?Leu?Lys?Glu?Gln?Asn?Met?Thr?Leu?Glu?Val?Ser?Asp?Glu?Ala
625 630 635 640
Lys?Glu?Lys?Ile?Ala?Glu?Ile?Gly?Tyr?His?Pro?Ala?Phe?Gly?Ala?Arg
645 650 655
Pro?Leu?Arg?Arg?Thr?Ile?Gln?Thr?Ala?Val?Glu?Asp?Gln?Met?Thr?Asp
660 665 670
Leu?Leu?Leu?Glu?Glu?Glu?His?Ile?Lys?Gly?Phe?Ala?Val?Ile?Val?Glu
675 680 685
Asn?Asp?Glu?Ile?Arg?Val?Lys?Thr?Thr?Arg
690 695
<210>7
<211>2958
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(2459)
<400>7
gctgacaaaa?gatgcgcggg?atgttcttcc?cgaaagcctc?tcttatccga?aagccgtgga 60
agcaagcgcg?gtcagcaata?tgctcgtcgc?cggactgatg?gcgaaagatt?ggaagcttgt 120
cggccgcatg?atgcaaaaag?acctctttca?ccagccatac?cgaagggcgc?tcgtccctga 180
gctttcgaaa?gtggagcatg?aagcaggcca?aaacggcgcg?ttcgggacgg?ctttaagcgg 240
agccggcccg?acgatccttt?cctttattga?aaaaggaaaa?ggagaggcgc?tgagaaacca 300
gcttgcgtcc?aagtttccgc?actgtgaggt?cgactgcctt?tatgtcccgg?atacaggcat 360
tatcgttgaa?agaaaatcag?taaacagtgt?ttaacgaaaa?aagagctttt?tcggctcttt 420
ttttgtttgc?cttcaaaaca?aggattttta?tttaggaagt?cgaagtagta?tggcaaacag 480
acatgaggag?gagaaacgac?atg?aag?cag?ctg?ata?acc?gaa?aaa?gac?ctc?atc 533
Met?Lys?Gln?Leu?Ile?Thr?Glu?Lys?Asp?Leu?Ile
1 5 10
aag?ctt?gta?tcc?att?acc?gat?ccc?cag?tat?tcg?ccc?gat?ggt?gca?aaa 581
Lys?Leu?Val?Ser?Ile?Thr?Asp?Pro?Gln?Tyr?Ser?Pro?Asp?Gly?Ala?Lys
15 20 25
atc?gcc?tac?gtc?caa?aea?aaa?gta?aac?gaa?aaa?cag?gac?tca?tat?gat 629
Ile?Ala?Tyr?Val?Gln?Thr?Lys?Val?Asn?Glu?Lys?Gln?Asp?Ser?Tyr?Asp
30 35 40
tcg?cat?atc?atg?atc?tac?gac?cgc?gaa?aaa?caa?gct?tcg?gtg?caa?tgg 677
Ser?His?Ile?Met?Ile?Tyr?Asp?Arg?Glu?Lys?Gln?Ala?Ser?Val?Gln?Trp
45 50 55
acg?ttt?ggg?aaa?ggc?aga?aac?cag?cat?ccc?cgc?tgg?tcg?ccg?gac?ggc 725
Thr?Phe?Gly?Lys?Gly?Arg?Asn?Gln?His?Pro?Arg?Trp?Ser?Pro?Asp?Gly
60 65 70 75
aaa?tac?ctt?gca?ttt?acg?tcc?aat?cgc?gag?gaa?acc?gca?caa?att?tac 773
Lys?Tyr?Leu?Ala?Phe?Thr?Ser?Asn?Arg?Glu?Glu?Thr?Ala?Gln?Ile?Tyr
80 85 90
gtg?ata?agc?gcc?gcc?ggg?gga?gaa?gcg?aga?aaa?gtg?acc?gat?att?ccc 821
Val?Ile?Ser?Ala?Ala?Gly?Gly?Glu?Ala?Arg?Lys?Val?Thr?Asp?Ile?Pro
95 100 105
tat?gat?gta?tca?cag?cct?gaa?tgg?tcg?ccg?gac?ggc?aag?tca?ttg?ctt 869
Tyr?Asp?Val?Ser?Gln?Pro?Glu?Trp?Ser?Pro?Asp?Gly?Lys?Ser?Leu?Leu
110 115 120
tgt?tca?gtg?aag?ctg?aca?aaa?gag?gaa?agc?gtc?gac?gat?gag?aag?aaa 917
Cys?Ser?Val?Lys?Leu?Thr?Lys?Glu?Glu?Ser?Val?Asp?Asp?Glu?Lys?Lys
125 130 135
acc?gag?att?gaa?gac?cac?gaa?ccg?ctg?gaa?gtc?gat?tct?ttg?tca?tat 965
Thr?Glu?Ile?Glu?Asp?His?Glu?Pro?Leu?Glu?Val?Asp?Ser?Leu?Ser?Tyr
140 145 150 155
aaa?gca?gac?ggc?caa?ggg?ttt?aaa?agg?gga?aag?tat?acg?cag?ctt?gtc 1013
Lys?Ala?Asp?Gly?Gln?Gly?Phe?Lys?Arg?Gly?Lys?Tyr?Thr?Gln?Leu?Val
160 165 170
ctc?gtt?caa?gtg?gaa?acc?ggg?gaa?atg?aaa?cag?ctg?acc?gat?ctt?gaa 1061
Leu?Val?Gln?Val?Glu?Thr?Gly?Glu?Met?Lys?Gln?Leu?Thr?Asp?Leu?Glu
175 180 185
cgc?gac?cat?ttc?agc?cat?gca?ttt?tca?ccg?tgc?gga?gat?cag?att?gca 1109
Arg?Asp?His?Phe?Ser?His?Ala?Phe?Ser?Pro?Cys?Gly?Asp?Gln?Ile?Ala
190 195 200
ttt?tgc?gcc?aat?caa?acg?gat?atg?cgt?gtg?aac?gat?gtc?tac?ttg?atg 1157
Phe?Cys?Ala?Asn?Gln?Thr?Asp?Met?Arg?Val?Asn?Asp?Val?Tyr?Leu?Met
205 210 215
aac?ctt?tcg?aca?ggt?gat?ttg?aag?cgc?ctc?aca?ggt?caa?aac?ggc?att 1205
Asn?Leu?Ser?Thr?Gly?Asp?Leu?Lys?Arg?Leu?Thr?Gly?Gln?Asn?Gly?Ile
220 225 230 235
ttt?tct?tca?cta?tcg?ttt?tct?cct?gac?ggc?aaa?cac?ctt?gca?ttt?atc 1253
Phe?Ser?Ser?Leu?Ser?Phe?Ser?Pro?Asp?Gly?Lys?His?Leu?Ala?Phe?Ile
240 245 250
gga?aac?gag?aaa?gaa?tat?caa?aac?gcc?acg?ctc?gat?aaa?gca?tgg?ctg 1301
Gly?Asn?Glu?Lys?Glu?Tyr?Gln?Asn?Ala?Thr?Leu?Asp?Lys?Ala?Trp?Leu
255 260 265
tat?gat?att?gaa?aca?gga?aaa?cta?acg?tgt?ctg?acg?gaa?atg?ctt?gac 1349
Tyr?Asp?Ile?Glu?Thr?Gly?Lys?Leu?Thr?Cys?Leu?Thr?Glu?Met?Leu?Asp
270 275 280
gtc?cat?ctc?agc?gat?gcg?gtg?gca?gga?gac?agc?ctt?gtc?gga?ggc?gtt 1397
Val?His?Leu?Ser?Asp?Ala?Val?Ala?Gly?Asp?Ser?Leu?Val?Gly?Gly?Val
285 290 295
ctt?ccg?aaa?ccg?gcc?tgg?acc?aaa?gac?gga?aac?ggc?ttt?tat?gtc?atc 1445
Leu?Pro?Lys?Pro?Ala?Trp?Thr?Lys?Asp?Gly?Asn?Gly?Phe?Tyr?Val?Ile
300 305 310 315
gga?tct?gac?cag?ggt?tct?aca?ggc?att?tac?tac?ata?tca?atc?gaa?ggt 1493
Gly?Ser?Asp?Gln?Gly?Ser?Thr?Gly?Ile?Tyr?Tyr?Ile?Ser?Ile?Glu?Gly
320 325 330
ctt?gct?tat?ccg?gtc?cgg?ttg?gaa?aaa?gag?cat?gtg?aac?gga?ttc?agc 1541
Leu?Ala?Tyr?Pro?Val?Arg?Leu?Glu?Lys?Glu?His?Val?Asn?Gly?Phe?Ser
335 340 345
ctg?cat?ccg?gac?gaa?agt?ggc?ttt?gcg?gca?tcc?ata?gcc?ctg?ccc?gtc 1589
Leu?His?Pro?Asp?Glu?Ser?Gly?Phe?Ala?Ala?Ser?Ile?Ala?Leu?Pro?Val
350 355 360
cgg?ccg?agc?gaa?ctt?tac?cat?ctc?caa?ttg?ggt?gaa?gaa?aaa?gcg?gag 1637
Arg?Pro?Ser?Glu?Leu?Tyr?His?Leu?Gln?Leu?Gly?Glu?Glu?Lys?Ala?Glu
365 370 375
cga?cta?aca?gat?gcc?aac?caa?gcg?ttt?acg?gaa?gaa?cat?atc?ata?tcg 1685
Arg?Leu?Thr?Asp?Ala?Asn?Gln?Ala?Phe?Thr?Glu?Glu?His?Ile?Ile?Ser
380 385 390 395
gaa?ccg?gag?gag?ctt?caa?ttt?cag?gca?gcg?gac?ggt?ctg?aca?att?cac 1733
Glu?Pro?Glu?Glu?Leu?Gln?Phe?Gln?Ala?Ala?Asp?Gly?Leu?Thr?Ile?His
400 405 410
gga?tgg?ctg?ata?aag?ccc?gcc?caa?tat?gaa?aaa?gga?aac?acg?tat?ccg 1781
Gly?Trp?Leu?Ile?Lys?Pro?Ala?Gln?Tyr?Glu?Lys?Gly?Asn?Thr?Tyr?Pro
415 420 425
ctc?att?ctg?gaa?gtg?cac?ggc?gga?cct?cac?gcc?atg?tat?gcg?aat?gct 1829
Leu?Ile?Leu?Glu?Val?His?Gly?Gly?Pro?His?Ala?Met?Tyr?Ala?Asn?Ala
430 435 440
tat?ttt?cat?gaa?ttt?caa?gtg?ctg?gcc?gca?aag?gga?agc?gcc?gtc?gta 1877
Tyr?Phe?His?Glu?Phe?Gln?Val?Leu?Ala?Ala?Lys?Gly?Ser?Ala?Val?Val
445 450 455
tat?gtt?aac?ccg?cgg?gga?agc?cac?ggc?tac?gga?cag?gat?ttt?gtc?aac 1925
Tyr?Val?Asn?Pro?Arg?Gly?Ser?His?Gly?Tyr?Gly?Gln?Asp?Phe?Val?Asn
460 465 470 475
cgg?gtg?agg?ggc?gac?tac?ggc?ggt?gga?gat?ttt?aag?gat?gta?atg?gcg 1973
Arg?Val?Arg?Gly?Asp?Tyr?Gly?Gly?Gly?Asp?Phe?Lys?Asp?Val?Met?Ala
480 485 490
gct?gtt?gac?cac?gtg?ctt?gaa?cat?tat?gat?ttc?gtc?gat?caa?gaa?agg 2021
Ala?Val?Asp?His?Val?Leu?Glu?His?Tyr?Asp?Phe?Val?Asp?Gln?Glu?Arg
495 500 505
ctc?ggc?atc?acc?gga?gga?agc?tac?ggg?ggc?ttt?atg?aca?aac?tgg?gct 2069
Leu?Gly?Ile?Thr?Gly?Gly?Ser?Tyr?Gly?Gly?Phe?Met?Thr?Asn?Trp?Ala
510 515 520
gtc?ggc?cac?acg?aag?cgc?ttc?aag?gcc?gca?gtg?aca?cag?agg?tcc?att 2117
Val?Gly?His?Thr?Lys?Arg?Phe?Lys?Ala?Ala?Val?Thr?Gln?Arg?Ser?Ile
525 530 535
tcc?aac?tgg?atc?agt?ttt?tac?ggg?gta?agc?gac?atc?ggc?tat?ttt?ttc 2165
Ser?Asn?Trp?Ile?Ser?Phe?Tyr?Gly?Val?Ser?Asp?Ile?Gly?Tyr?Phe?Phe
540 545 550 555
aca?gac?tgg?cag?ctc?ggt?gca?gac?ctt?ttt?gaa?gat?cca?ggc?aaa?ctg 2213
Thr?Asp?Trp?Gln?Leu?Gly?Ala?Asp?Leu?Phe?Glu?Asp?Pro?Gly?Lys?Leu
560 565 570
tgg?gag?cat?tcg?ccg?ctc?aaa?tac?gcg?gac?aag?gtg?gag?acc?cct?ctt 2261
Trp?Glu?His?Ser?Pro?Leu?Lys?Tyr?Ala?Asp?Lys?Val?Glu?Thr?Pro?Leu
575 580 585
ctc?att?ctg?cat?ggc?gaa?cgg?gac?gac?agg?tgc?ccg?att?gaa?cag?gcg 2309
Leu?Ile?Leu?His?Gly?Glu?Arg?Asp?Asp?Arg?Cys?Pro?Ile?Glu?Gln?Ala
590 595 600
gaa?cag?ctg?ttc?acg?gcg?ctg?aaa?aaa?atg?ggg?aaa?gaa?gta?aaa?ctc 2357
Glu?Gln?Leu?Phe?Thr?Ala?Leu?Lys?Lys?Met?Gly?Lys?Glu?Val?Lys?Leu
605 610 615
gtc?aga?ttt?ccg?aac?gcg?tca?cac?gat?tta?tca?aga?agc?ggg?cat?ccg 2405
Val?Arg?Phe?Pro?Asn?Ala?Ser?His?Asp?Leu?Ser?Arg?Ser?Gly?His?Pro
620 625 630 635
aag?cag?cgg?atc?agg?cgg?ctt?gag?tat?att?gca?ggc?tgg?ttt?gaa?gct 2453
Lys?Gln?Arg?Ile?Arg?Arg?Leu?Glu?Tyr?Ile?Ala?Gly?Trp?Phe?Glu?Ala
640 645 650
tat?cta?taaaaaagaa?aaggctgccg?tacactacgg?cagcctcagc?gtgtcgacaa 2509
Tyr?Leu
accctcgcat?tcgttgtcag?ggctgcgcgt?cggtgctcac?gaattccaac?attcgctccg 2569
ctccgatgct?cgaccttcct?agactgcaag?ggttttcaat?cacgctgaga?ggatgacaaa 2629
atcctaaact?gaaaaccgtt?ttaggatttt?gtcaacaatt?taggctgccg?ttacggcagc 2689
cttctcaaat?taaaatactt?gctctacttc?aatgacgccc?ggaacttctt?ctaaaagggc 2749
gcgctcaatt?ccagctttaa?gggtgatggt?ggaacttggg?cagcttccgc?atgcgccgag 2809
caggcgaagc?ttgacgatgc?cgtcttcaac?atcgacgagt?tcacagtcac?cgccgtcacg 2869
gagtaaaaac?ggacgaagtt?tgtccagcac?ttcctgtact?tggtccttca?tttcgacctc 2929
tgtcgccatt?cgtatcgctc?cttttaaat 2958
<210>8
<211>653
<212>PRT
＜213〉Bacillus licheniformis
<400>8
Met?Lys?Gln?Leu?Ile?Thr?Glu?Lys?Asp?Leu?Ile?Lys?Leu?Val?Ser?Ile
1 5 10 15
Thr?Asp?Pro?Gln?Tyr?Ser?Pro?Asp?Gly?Ala?Lys?Ile?Ala?Tyr?Val?Gln
20 25 30
Thr?Lys?Val?Asn?Glu?Lys?Gln?Asp?Ser?Tyr?Asp?Ser?His?Ile?Met?Ile
35 40 45
Tyr?Asp?Arg?Glu?Lys?Gln?Ala?Ser?Val?Gln?Trp?Thr?Phe?Gly?Lys?Gly
50 55 60
Arg?Asn?Gln?His?Pro?Arg?Trp?Ser?Pro?Asp?Gly?Lys?Tyr?Leu?Ala?Phe
65 70 75 80
Thr?Ser?Asn?Arg?Glu?Glu?Thr?Ala?Gln?Ile?Tyr?Val?Ile?Ser?Ala?Ala
85 90 95
Gly?Gly?Glu?Ala?Arg?Lys?Val?Thr?Asp?Ile?Pro?Tyr?Asp?Val?Ser?Gln
100 105 110
Pro?Glu?Trp?Ser?Pro?Asp?Gly?Lys?Ser?Leu?Leu?Cys?Ser?Val?Lys?Leu
115 120 125
Thr?Lys?Glu?Glu?Ser?Val?Asp?Asp?Glu?Lys?Lys?Thr?Glu?Ile?Glu?Asp
130 135 140
His?Glu?Pro?Leu?Glu?Val?Asp?Ser?Leu?Ser?Tyr?Lys?Ala?Asp?Gly?Gln
145 150 155 160
Gly?Phe?Lys?Arg?Gly?Lys?Tyr?Thr?Gln?Leu?Val?Leu?Val?Gln?Val?Glu
165 170 175
Thr?Gly?Glu?Met?Lys?Gln?Leu?Thr?Asp?Leu?Glu?Arg?Asp?His?Phe?Ser
180 185 190
His?Ala?Phe?Ser?Pro?Cys?Gly?Asp?Gln?Ile?Ala?Phe?Cys?Ala?Asn?Gln
195 200 205
Thr?Asp?Met?Arg?Val?Asn?Asp?Val?Tyr?Leu?Met?Asn?Leu?Ser?Thr?Gly
210 215 220
Asp?Leu?Lys?Arg?Leu?Thr?Gly?Gln?Asn?Gly?Ile?Phe?Ser?Ser?Leu?Ser
225 230 235 240
Phe?Ser?Pro?Asp?Gly?Lys?His?Leu?Ala?Phe?Ile?Gly?Asn?Glu?Lys?Glu
245 250 255
Tyr?Gln?Asn?Ala?Thr?Leu?Asp?Lys?Ala?Trp?Leu?Tyr?Asp?Ile?Glu?Thr
260 265 270
Gly?Lys?Leu?Thr?Cys?Leu?Thr?Glu?Met?Leu?Asp?Val?His?Leu?Ser?Asp
275 280 285
Ala?Val?Ala?Gly?Asp?Ser?Leu?Val?Gly?Gly?Val?Leu?Pro?Lys?Pro?Ala
290 295 300
Trp?Thr?Lys?Asp?Gly?Asn?Gly?Phe?Tyr?Val?Ile?Gly?Ser?Asp?Gln?Gly
305 310 315 320
Ser?Thr?Gly?Ile?Tyr?Tyr?Ile?Ser?Ile?Glu?Gly?Leu?Ala?Tyr?Pro?Val
325 330 335
Arg?Leu?Glu?Lys?Glu?His?Val?Asn?Gly?Phe?Ser?Leu?His?Pro?Asp?Glu
340 345 350
Ser?Gly?Phe?Ala?Ala?Ser?Ile?Ala?Leu?Pro?Val?Arg?Pro?Ser?Glu?Leu
355 360 365
Tyr?His?Leu?Gln?Leu?Gly?Glu?Glu?Lys?Ala?Glu?Arg?Leu?Thr?Asp?Ala
370 375 380
Asn?Gln?Ala?Phe?Thr?Glu?Glu?His?Ile?Ile?Ser?Glu?Pro?Glu?Glu?Leu
385 390 395 400
Gln?Phe?Gln?Ala?Ala?Asp?Gly?Leu?Thr?Ile?His?Gly?Trp?Leu?Ile?Lys
405 410 415
Pro?Ala?Gln?Tyr?Glu?Lys?Gly?Asn?Thr?Tyr?Pro?Leu?Ile?Leu?Glu?Val
420 425 430
His?Gly?Gly?Pro?His?Ala?Met?Tyr?Ala?Asn?Ala?Tyr?Phe?His?Glu?Phe
435 440 445
Gln?Val?Leu?Ala?Ala?Lys?Gly?Ser?Ala?Val?Val?Tyr?Val?Asn?Pro?Arg
450 455 460
Gly?Ser?His?Gly?Tyr?Gly?Gln?Asp?Phe?Val?Asn?Arg?Val?Arg?Gly?Asp
465 470 475 480
Tyr?Gly?Gly?Gly?Asp?Phe?Lys?Asp?Val?Met?Ala?Ala?Val?Asp?His?Val
485 490 495
Leu?Glu?His?Tyr?Asp?Phe?Val?Asp?Gln?Glu?Arg?Leu?Gly?Ile?Thr?Gly
500 505 5l0
Gly?Ser?Tyr?Gly?Gly?Phe?Met?Thr?Asn?Trp?Ala?Val?Gly?His?Thr?Lys
515 520 525
Arg?Phe?Lys?Ala?Ala?Val?Thr?Gln?Arg?Ser?Ile?Ser?Asn?Trp?Ile?Ser
530 535 540
Phe?Tyr?Gly?Val?Ser?Asp?Ile?Gly?Tyr?Phe?Phe?Thr?Asp?Trp?Gln?Leu
545 550 555 560
Gly?Ala?Asp?Leu?Phe?Glu?Asp?Pro?Gly?Lys?Leu?Trp?Glu?His?Ser?Pro
565 570 575
Leu?Lys?Tyr?Ala?Asp?Lys?Val?Glu?Thr?Pro?Leu?Leu?Ile?Leu?His?Gly
580 585 590
Glu?Arg?Asp?Asp?Arg?Cys?Pro?Ile?Glu?Gln?Ala?Glu?Gln?Leu?Phe?Thr
595 600 605
Ala?Leu?Lys?Lys?Met?Gly?Lys?Glu?Val?Lys?Leu?Val?Arg?Phe?Pro?Asn
610 615 620
Ala?Ser?His?Asp?Leu?Ser?Arg?Ser?Gly?His?Pro?Lys?Gln?Arg?Ile?Arg
625 630 635 640
Arg?Leu?Glu?Tyr?Ile?Ala?Gly?Trp?Phe?Glu?Ala?Tyr?Leu
645 650
<210>9
<211>2473
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(148)..(1974)
<400>9
tatgtatttt?attttttaac?ggcgggattt?tgtgtctttt?ttttcgcata?tgatttgaca 60
aaagacggtg?aaaaaacaga?agatgaacac?aatacaagtt?tattggataa?caatggactt 120
ggagtaaaga?aagcgagggg?attgaca?gtg?gct?gaa?gaa?aaa?aaa?tcg?aag?aaa 174
Val?Ala?Glu?Glu?Lys?Lys?Ser?Lys?Lys
1 5
ctg?cct?tca?aga?gaa?gag?gtg?aag?caa?gaa?gac?acg?tgg?aga?ctg?gag 222
Leu?Pro?Ser?Arg?Glu?Glu?Val?Lys?Gln?Glu?Asp?Thr?Trp?Arg?Leu?Glu
10 15 20 25
gat?atc?ttc?ccg?tca?gat?gac?gct?tgg?aac?gag?gaa?ttt?caa?gcc?gtt 270
Asp?Ile?Phe?Pro?Ser?Asp?Asp?Ala?Trp?Asn?Glu?Glu?Phe?Gln?Ala?Val
30 35 40
aaa?gaa?ctg?ctg?ccc?aag?ctt?tcc?gaa?ttc?aag?ggc?aag?ctc?ggc?cat 318
Lys?Glu?Leu?Leu?Pro?Lys?Leu?Ser?Glu?Phe?Lys?Gly?Lys?Leu?Gly?His
45 50 55
tcc?gcc?gat?gat?tta?tat?gaa?gcg?ctg?act?tat?cag?gat?aag?gtg?atg 366
Ser?Ala?Asp?Asp?Leu?Tyr?Glu?Ala?Leu?Thr?Tyr?Gln?Asp?Lys?Val?Met
60 65 70
gag?cgt?ctc?ggg?aag?ctg?tat?act?tac?gct?cat?atg?cgc?tat?gat?cag 414
G1u?Arg?Leu?Gly?Lys?Leu?Tyr?Thr?Tyr?Ala?His?Met?Arg?Tyr?Asp?Gln
75 80 85
gat?acg?gga?aat?tct?ttt?tac?caa?ggt?ctc?aat?gat?aaa?gcg?gcg?aac 462
Asp?Thr?Gly?Asn?Ser?Phe?Tyr?Gln?Gly?Leu?Asn?Asp?Lys?Ala?Ala?Asn
90 95 100 105
ctc?tat?act?cag?gct?gcg?agc?gcc?acg?gcc?tac?atg?gtg?ccg?gaa?ata 510
Leu?Tyr?Thr?Gln?Ala?Ala?Ser?Ala?Thr?Ala?Tyr?Met?Val?Pro?Glu?Ile
110 115 120
tta?tcg?att?cag?gaa?gaa?aag?ctt?cag?cag?ttc?ctg?ttg?gaa?aaa?gag 558
Leu?Ser?Ile?Gln?Glu?Glu?Lys?Leu?Gln?Gln?Phe?Leu?Leu?Glu?Lys?Glu
125 130 135
gaa?tta?aag?ctt?tac?tca?cac?gct?ctc?gaa?gaa?atc?aat?aaa?gag?cgc 606
Glu?Leu?Lys?Leu?Tyr?Ser?His?Ala?Leu?Glu?Glu?Ile?Asn?Lys?Glu?Arg
140 145 150
cct?cat?gtg?ctt?agc?gag?gaa?gag?gaa?ggg?att?ttg?gcc?gaa?gcg?tct 654
Pro?His?Val?Leu?Ser?Glu?Glu?Glu?Glu?Gly?Ile?Leu?Ala?Glu?Ala?Ser
155 160 165
gat?gtt?ctt?tca?tct?cct?tcc?aat?aca?ttc?ggc?atg?ctg?aat?aac?gcc 702
Asp?Val?Leu?Ser?Ser?Pro?Ser?Asn?Thr?Phe?Gly?Met?Leu?Asn?Asn?Ala
170 175 180 185
gat?atg?gaa?ttt?ccg?gag?atc?acc?gat?gaa?aac?gga?gaa?aag?gtg?cag 750
Asp?Met?Glu?Phe?Pro?Glu?Ile?Thr?Asp?Glu?Asn?Gly?Glu?Lys?Val?Gln
190 195 200
ctc?aca?cac?ggc?aat?tat?atc?acc?ttt?ttg?gaa?agc?gag?aac?cgc?gat 798
Leu?Thr?His?Gly?Asn?Tyr?Ile?Thr?Phe?Leu?Glu?Ser?Glu?Asn?Arg?Asp
205 210 215
gtc?cgc?cgc?gcg?gca?ttc?aag?gcc?gtt?tat?gaa?aca?tac?ggc?cgc?ttt 846
Val?Arg?Arg?Ala?Ala?Phe?Lys?Ala?Val?Tyr?Glu?Thr?Tyr?Gly?Arg?Phe
220 225 230
aaa?aac?acg?cta?gct?tcc?acg?ctc?agc?gga?gct?gtg?aaa?aaa?gac?aac 894
Lys?Asn?Thr?Leu?Ala?Ser?Thr?Leu?Ser?Gly?Ala?Val?Lys?Lys?Asp?Asn
235 240 245
ttt?tat?gcg?aag?gtt?aaa?cat?tat?aaa?tcg?gcg?cgg?gaa?gct?gcc?ttg 942
Phe?Tyr?Ala?Lys?Val?Lys?His?Tyr?Lys?Ser?Ala?Arg?Glu?Ala?Ala?Leu
250 255 260 265
tcg?cgc?aac?agc?att?cct?gaa?gaa?gtc?tac?gac?aat?ctt?gtc?agc?acg 990
Ser?Arg?Asn?Ser?Ile?Pro?Glu?Glu?Val?Tyr?Asp?Asn?Leu?Val?Ser?Thr
270 275 280
atc?aac?aag?cat?ctc?ccg?ctt?ctt?cac?cgc?tat?gtt?gag?att?aga?aaa 1038
Ile?Asn?Lys?His?Leu?Pro?Leu?Leu?His?Arg?Tyr?Val?Glu?Ile?Arg?Lys
285 290 295
aaa?gtg?ctt?gag?ctt?gat?gaa?gtg?cac?atg?tat?gac?ctg?tat?acc?cct 1086
Lys?Val?Leu?Glu?Leu?Asp?Glu?Val?His?Met?Tyr?Asp?Leu?Tyr?Thr?Pro
300 305 310
ctg?gtc?aag?gat?tct?gga?atg?aaa?gtc?act?tac?gag?cag?gca?aag?gac 1134
Leu?Val?Lys?Asp?Ser?Gly?Met?Lys?Val?Thr?Tyr?Glu?Gln?Ala?Lys?Asp
315 320 325
tat?atg?ctg?aag?ggt?ctg?gcg?cct?ttg?ggg?gaa?gaa?tat?tca?tcc?atc 1182
Tyr?Met?Leu?Lys?Gly?Leu?Ala?Pro?Leu?Gly?Glu?Glu?Tyr?Ser?Ser?Ile
330 335 340 345
ctg?aaa?gaa?gga?ttg?aac?aac?cgc?tgg?gtc?gat?gtg?tat?gaa?aac?aaa 1230
Leu?Lys?Glu?Gly?Leu?Asn?Asn?Arg?Trp?Val?Asp?Val?Tyr?Glu?Asn?Lys
350 355 360
gga?aaa?cga?agc?ggc?gcc?tat?tct?tca?gga?act?tac?gga?acc?aat?ccg 1278
Gly?Lys?Arg?Ser?Gly?Ala?Tyr?Ser?Ser?Gly?Thr?Tyr?Gly?Thr?Asn?Pro
365 370 375
tat?att?ttg?atg?aac?tgg?cag?gac?aac?gtc?aac?aat?ctg?ttt?acg?ctc 1326
Tyr?Ile?Leu?Met?Asn?Trp?Gln?Asp?Asn?Val?Asn?Asn?Leu?Phe?Thr?Leu
380 385 390
gtc?cat?gaa?ttc?ggc?cat?tcc?gtg?cac?agc?tac?tat?acg?aga?aaa?tat 1374
Val?His?Glu?Phe?Gly?His?Ser?Val?His?Ser?Tyr?Tyr?Thr?Arg?Lys?Tyr
395 400 405
cag?cct?tac?ccg?tac?ggt?aac?tac?agc?att?ttc?gta?gcc?gaa?gtt?gct 1422
Gln?Pro?Tyr?Pro?Tyr?Gly?Asn?Tyr?Ser?Ile?Phe?Val?Ala?Glu?Val?Ala
410 415 420 425
tct?acg?acg?aat?gaa?gcg?ctg?ttg?gga?gaa?tac?ctg?ctg?aat?aca?atc 1470
Ser?Thr?Thr?Asn?Glu?Ala?Leu?Leu?Gly?Glu?Tyr?Leu?Leu?Asn?Thr?Ile
430 435 440
gaa?gat?gaa?aaa?cag?cgg?ctc?tat?att?ttg?aat?cat?atg?ctc?gaa?ggc 1518
Glu?Asp?Glu?Lys?Gln?Arg?Leu?Tyr?Ile?Leu?Asn?His?Met?Leu?Glu?Gly
445 450 455
ttt?aaa?gga?acg?gtt?ttc?aga?cag?acg?atg?ttt?gcc?gaa?ttt?gaa?cat 1566
Phe?Lys?Gly?Thr?Val?Phe?Arg?Gln?Thr?Met?Phe?Ala?Glu?Phe?Glu?His
460 465 470
gag?atc?cat?gtc?aaa?gcc?cag?gaa?ggg?gaa?ccg?ctg?aca?cct?gag?ctg 1614
Glu?Ile?His?Val?Lys?Ala?Gln?Glu?Gly?Glu?Pro?Leu?Thr?Pro?Glu?Leu
475 480 485
tta?acg?agc?ata?tac?tat?gat?ttg?aat?aag?aaa?tat?ttc?gga?gac?aat 1662
Leu?Thr?Ser?Ile?Tyr?Tyr?Asp?Leu?Asn?Lys?Lys?Tyr?Phe?Gly?Asp?Asn
490 495 500 505
att?gag?att?gat?aag?gaa?atc?ggt?ctt?gag?tgg?tca?aga?att?ccg?cat 1710
Ile?Glu?Ile?Asp?Lys?Glu?Ile?Gly?Leu?Glu?Trp?Ser?Arg?Ile?Pro?His
510 515 520
ttc?tat?tac?aat?tat?tat?gta?tat?cag?tat?gcg?aca?ggc?ttc?agt?gca 1758
Phe?Tyr?Tyr?Asn?Tyr?Tyr?Val?Tyr?Gln?Tyr?Ala?Thr?Gly?Phe?Ser?Ala
525 530 535
gct?cag?gcg?ctc?agc?cag?cag?att?tta?aaa?gaa?ggc?aaa?tcg?gct?gtt 1806
Ala?Gln?Ala?Leu?Ser?Gln?Gln?Ile?Leu?Lys?Glu?Gly?Lys?Ser?Ala?Val
540 545 550
gac?cgc?tat?att?gag?ttc?ttg?aaa?gct?gga?agc?tcg?gat?tat?ccg?att 1854
Asp?Arg?Tyr?Ile?Glu?Phe?Leu?Lys?Ala?Gly?Ser?Ser?Asp?Tyr?Pro?Ile
555 560 565
gaa?gtg?ctg?aaa?aaa?gcc?ggc?gtc?gat?atg?acg?tct?tca?gag?ccg?atc 1902
Glu?Val?Leu?Lys?Lys?Ala?Gly?Val?Asp?Met?Thr?Ser?Ser?Glu?Pro?Ile
570 575 580 585
gaa?gct?gcg?tgc?aaa?aag?ttt?gaa?gag?cag?ctg?aat?gaa?atg?gaa?gaa 1950
Glu?Ala?Ala?Cys?Lys?Lys?Phe?Glu?Glu?Gln?Leu?Asn?Glu?Met?Glu?Glu
590 595 600
ctc?ctg?caa?aaa?gtc?aat?cat?tca?taaaaggcgt?atgcatgaaa?gaggctgccg 2004
Leu?Leu?Gln?Lys?Val?Asn?His?Ser
605
gtctaccggc?agccttgttc?gcgatgatgt?caaaaacctt?taaacgtttt?gcggtggttg 2064
aggcggctga?tcgtaaaaag?gattgatata?aataataggg?gagacgtaat?ataaaggggt 2124
agcatattca?tcaaacctaa?aatattcaag?acgaaaaaaa?ataaaattga?tgcaagtccc 2184
aaaatgatct?gaagcataaa?aaccctccta?aggactgata?aaatattaag?ggggcgacga 2244
agcgcaataa?gaccggcaaa?accgggcgcc?agtccgcata?attcggggtt?tggcagcgtg 2304
cgtcgggctc?gttttctttg?ttactttgat?catatgatgg?acatctatta?tttatgtttt 2364
tgtgacaaaa?atgt?gaaaac?taatgaaatc?agttgtcaaa?actcgacatg?atttgatatt?2424
ataaagatgt?gaaattgatc?acaaacaaac?atttacccct?ttgtttgac 2473
<210>10
<211>609
<212>PRT
＜213〉Bacillus licheniformis
<400>10
Val?Ala?Glu?Glu?Lys?Lys?Ser?Lys?Lys?Leu?Pro?Ser?Arg?Glu?Glu?Val
1 5 10 15
Lys?Gln?Glu?Asp?Thr?Trp?Arg?Leu?Glu?Asp?Ile?Phe?Pro?Ser?Asp?Asp
20 25 30
Ala?Trp?Asn?Glu?Glu?Phe?Gln?Ala?Val?Lys?Glu?Leu?Leu?Pro?Lys?Leu
35 40 45
Ser?Glu?Phe?Lys?Gly?Lys?Leu?Gly?His?Ser?Ala?Asp?Asp?Leu?Tyr?Glu
50 55 60
Ala?Leu?Thr?Tyr?Gln?Asp?Lys?Val?Met?Glu?Arg?Leu?Gly?Lys?Leu?Tyr
65 70 75 80
Thr?Tyr?Ala?His?Met?Arg?Tyr?Asp?Gln?Asp?Thr?Gly?Asn?Ser?Phe?Tyr
85 90 95
Gln?Gly?Leu?Asn?Asp?Lys?Ala?Ala?Asn?Leu?Tyr?Thr?Gln?Ala?Ala?Ser
100 105 110
Ala?Thr?Ala?Tyr?Met?Val?Pro?Glu?Ile?Leu?Ser?Ile?Gln?Glu?Glu?Lys
115 120 125
Leu?Gln?Gln?Phe?Leu?Leu?Glu?Lys?Glu?Glu?Leu?Lys?Leu?Tyr?Ser?His
130 135 140
Ala?Leu?Glu?Glu?Ile?Asn?Lys?Glu?Arg?Pro?His?Val?Leu?Ser?Glu?Glu
145 150 155 160
Glu?Glu?Gly?Ile?Leu?Ala?Glu?Ala?Ser?Asp?Val?Leu?Ser?Ser?Pro?Ser
165 170 175
Asn?Thr?Phe?Gly?Met?Leu?Asn?Asn?Ala?Asp?Met?Glu?Phe?Pro?Glu?Ile
180 185 190
Thr?Asp?Glu?Asn?Gly?Glu?Lys?Val?Gln?Leu?Thr?His?Gly?Asn?Tyr?Ile
195 200 205
Thr?Phe?Leu?Glu?Ser?Glu?Asn?Arg?Asp?Val?Arg?Arg?Ala?Ala?Phe?Lys
210 215 220
Ala?Val?Tyr?Glu?Thr?Tyr?Gly?Arg?Phe?Lys?Asn?Thr?Leu?Ala?Ser?Thr
225 230 235 240
Leu?Ser?Gly?Ala?Val?Lys?Lys?Asp?Asn?Phe?Tyr?Ala?Lys?Val?Lys?His
245 250 255
Tyr?Lys?Ser?Ala?Arg?Glu?Ala?Ala?Leu?Ser?Arg?Asn?Ser?Ile?Pro?Glu
260 265 270
Glu?Val?Tyr?Asp?Asn?Leu?Val?Ser?Thr?Ile?Asn?Lys?His?Leu?Pro?Leu
275 280 285
Leu?His?Arg?Tyr?Val?Glu?Ile?Arg?Lys?Lys?Val?Leu?Glu?Leu?Asp?Glu
290 295 300
Val?His?Met?Tyr?Asp?Leu?Tyr?Thr?Pro?Leu?Val?Lys?Asp?Ser?Gly?Met
305 3I0 315 320
Lys?Val?Thr?Tyr?Glu?Gln?Ala?Lys?Asp?Tyr?Met?Leu?Lys?Gly?Leu?Ala
325 330 335
Pro?Leu?Gly?Glu?Glu?Tyr?Ser?Ser?Ile?Leu?Lys?Glu?Gly?Leu?Asn?Asn
340 345 350
Arg?Trp?Val?Asp?Val?Tyr?Glu?Asn?Lys?Gly?Lys?Arg?Ser?Gly?Ala?Tyr
355 360 365
Ser?Ser?Gly?Thr?Tyr?Gly?Thr?Asn?Pro?Tyr?Ile?Leu?Met?Asn?Trp?Gln
370 375 380
Asp?Asn?Val?Asn?Asn?Leu?Phe?Thr?Leu?Val?His?Glu?Phe?Gly?His?Ser
385 390 395 400
Val?His?Ser?Tyr?Tyr?Thr?Arg?Lys?Tyr?Gln?Pro?Tyr?Pro?Tyr?Gly?Asn
405 410 415
Tyr?Ser?Ile?Phe?Val?Ala?Glu?Val?Ala?Ser?Thr?Thr?Asn?Glu?Ala?Leu
420 425 430
Leu?Gly?Glu?Tyr?Leu?Leu?Asn?Thr?Ile?Glu?Asp?Glu?Lys?Gln?Arg?Leu
435 440 445
Tyr?Ile?Leu?Asn?His?Met?Leu?Glu?Gly?Phe?Lys?Gly?Thr?Val?Phe?Arg
450 455 460
Gln?Thr?Met?Phe?Ala?Glu?Phe?Glu?His?Glu?Ile?His?Val?Lys?Ala?Gln
465 470 475 480
Glu?Gly?Glu?Pro?Leu?Thr?Pro?Glu?Leu?Leu?Thr?Ser?Ile?Tyr?Tyr?Asp
485 490 495
Leu?Asn?Lys?Lys?Tyr?Phe?Gly?Asp?Asn?Ile?Glu?Ile?Asp?Lys?Glu?Ile
500 505 510
Gly?Leu?Glu?Trp?Ser?Arg?Ile?Pro?His?Phe?Tyr?Tyr?Asn?Tyr?Tyr?Val
515 520 525
Tyr?Gln?Tyr?Ala?Thr?Gly?Phe?Ser?Ala?Ala?Gln?Ala?Leu?Ser?Gln?Gln
530 535 540
Ile?Leu?Lys?Glu?Gly?Lys?Ser?Ala?Val?Asp?Arg?Tyr?lle?Glu?Phe?Leu
545 550 555 560
Lys?Ala?Gly?Ser?Ser?Asp?Tyr?Pro?Ile?Glu?Val?Leu?Lys?Lys?Ala?Gly
565 570 575
Val?Asp?Met?Thr?Ser?Ser?Glu?Pro?Ile?Glu?Ala?Ala?Cys?Lys?Lys?Phe
580 585 590
Glu?Glu?Gln?Leu?Asn?Glu?Met?Glu?Glu?Leu?Leu?Gln?Lys?Val?Asn?His
595 600 605
Ser
<210>11
<211>2746
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(491)..(2245)
<400>11
atctcctttt?gtctcatact?cttttaaggt?atccgcgaat?tctttaaggg?ttgtctgatt 60
cgtcacttta?tagttaacaa?aatactcagc?tgtttcatct?aaattttttg?caatttccgt 120
caaaagcacg?gaaaacttgt?ctttttttct?tttcaacatt?tgtaaccctc?cattgtttaa 180
atgagacaat?gcacacatca?aaatattata?taactttttg?tcgaaaaaaa?acaggtgcag 240
tcgaaaaaaa?caattaattt?tcaaatcgtc?atacctttta?gataggcgca?agcaccagct 300
ggcatccggg?attttaagat?gacaataggc?gatttttcct?ttgatatcat?cgttccaaaa 360
gaacggatct?caggatcgtt?tcgagaaatt?tgggagttta?ggttcaaaaa?aatagaaaat 420
tgggagtttt?tcttattttc?agtccgcttt?cctgttatag?tagtaataaa?ctggaaagga 480
aggtgatctg?atg?aga?cgg?tta?gct?ttc?tta?gtt?gtt?gcg?ttt?tgc?ttg 529
Met?Arg?Arg?Leu?Ala?Phe?Leu?Val?Val?Ala?Phe?Cys?Leu
1 5 10
gcg?gtt?ggt?tgc?ttt?ttc?agt?ccg?gtg?tcg?aaa?gca?gaa?gga?gtc?atg 577
Ala?Val?Gly?Cys?Phe?Phe?Ser?Pro?Val?Ser?Lys?Ala?Glu?Gly?Val?Met
15 20 25
agc?ggg?gga?gat?ggt?gat?aaa?gtc?gcc?gtc?ggg?aaa?gac?ggt?atg?gtg 625
Ser?Gly?Gly?Asp?Gly?Asp?Lys?Val?Ala?Val?Gly?Lys?Asp?Gly?Met?Val
30 35 40 45
gct?acg?gcc?cat?ccg?ctt?gca?tca?aaa?atc?gga?gcc?gaa?gtt?ttg?aaa 673
Ala?Thr?Ala?His?Pro?Leu?Ala?Ser?Lys?Ile?Gly?Ala?Glu?Val?Leu?Lys
50 55 60
aag?ggc?ggg?aat?gcc?att?gat?gca?gct?att?gcg?atc?cag?tac?gca?ctg 721
Lys?Gly?Gly?Asn?Ala?Ile?Asp?Ala?Ala?Ile?Ala?Ile?Gln?Tyr?Ala?Leu
65 70 75
aat?gta?aca?gag?ccg?atg?atg?tct?gga?atc?ggc?ggc?ggc?ggt?ttc?atg 769
Asn?Val?Thr?Glu?Pro?Met?Met?Ser?Gly?Ile?Gly?Gly?Gly?Gly?Phe?Met
80 85 90
atg?gtc?tac?gac?ggc?gag?acg?aag?gaa?acg?tcg?atc?atc?aac?agc?agg 817
Met?Val?Tyr?Asp?Gly?Glu?Thr?Lys?Glu?Thr?Ser?Ile?Ile?Asn?Ser?Arg
95 100 105
gag?cgt?gct?ccg?gag?gga?gcc?aag?cct?gac?atg?ttt?ttg?gat?gaa?gac 865
Glu?Arg?Ala?Pro?Glu?Gly?Ala?Lys?Pro?Asp?Met?Phe?Leu?Asp?Glu?Asp
110 115 120 125
gga?aag?gtg?att?ccg?ttt?tca?gaa?agg?tcg?aga?cac?gga?aat?gcg?gtc 913
Gly?Lys?Val?Ile?Pro?Phe?Ser?Glu?Arg?Ser?Arg?His?Gly?Asn?Ala?Val
130 135 140
ggt?gtg?ccg?gga?acg?ctg?aaa?ggg?ctt?gaa?gca?gcg?cat?aag?aag?tgg 961
Gly?Val?Pro?Gly?Thr?Leu?Lys?Gly?Leu?Glu?Ala?Ala?His?Lys?Lys?Trp
145 150 155
gga?aca?aag?aaa?atg?gaa?gac?ttg?att?tcg?cct?tct?ata?aag?ctt?gca 1009
Gly?Thr?Lys?Lys?Met?Glu?Asp?Leu?Ile?Ser?Pro?Ser?Ile?Lys?Leu?Ala
160 165 170
gag?gaa?ggg?ttc?cca?atc?gac?tcg?gtt?ttg?gct?gac?gcg?ata?aag?gat 1057
Glu?Glu?Gly?Phe?Pro?Ile?Asp?Ser?Val?Leu?Ala?Asp?Ala?Ile?Lys?Asp
175 180 185
cat?cag?gac?aag?ctg?agc?aaa?acg?gcc?gca?aag?gat?att?ttc?ctt?ccg 1105
His?Gln?Asp?Lys?Leu?Ser?Lys?Thr?Ala?Ala?Lys?Asp?Ile?Phe?Leu?Pro
190 195 200 205
gac?ggt?gaa?cct?ttg?aaa?gaa?ggc?gat?atc?ctt?gta?caa?aag?gat?ttg 1153
Asp?Gly?Glu?Pro?Leu?Lys?Glu?Gly?Asp?Ile?Leu?Val?Gln?Lys?Asp?Leu
210 215 220
gcc?aaa?acg?ttt?aaa?ttg?ata?cgg?aaa?gaa?ggg?tca?aaa?gcc?ttt?tat 1201
Ala?Lys?Thr?Phe?Lys?Leu?Ile?Arg?Lys?Glu?Gly?Ser?Lys?Ala?Phe?Tyr
225 230 235
gat?ggg?gaa?atc?ggc?cgg?gcg?att?gca?gat?gtc?gtg?cag?gat?ttc?ggg 1249
Asp?Gly?Glu?Ile?Gly?Arg?Ala?Ile?Ala?Asp?Val?Val?Gln?Asp?Phe?Gly
240 245 250
ggt?tca?atg?aca?cct?gat?gac?ctg?tcc?cgc?tac?gag?gtc?aca?aca?gat 1297
Gly?Ser?Met?Thr?Pro?Asp?Asp?Leu?Ser?Arg?Tyr?Glu?Val?Thr?Thr?Asp
255 260 265
aag?ccc?atc?tgg?ggc?gag?tac?cac?ggc?tat?gat?att?gca?agc?atg?cct 1345
Lys?Pro?Ile?Trp?Gly?Glu?Tyr?His?Gly?Tyr?Asp?Ile?Ala?Ser?Met?Pro
270 275 280 285
cct?cca?agc?tca?ggc?gga?gtg?ttc?atg?ctg?cag?atg?ctg?aaa?ttg?att 1393
Pro?Pro?Ser?Ser?Gly?Gly?Val?Phe?Met?Leu?Gln?Met?Leu?Lys?Leu?Ile
290 295 300
gat?gat?ttt?cat?tta?tcg?caa?tac?gat?cca?aag?tcg?ttt?gaa?aaa?tat 1441
Asp?Asp?Phe?His?Leu?Ser?Gln?Tyr?Asp?Pro?Lys?Ser?Phe?Glu?Lys?Tyr
305 310 315
cat?ctg?ctc?gcg?gaa?acg?atg?cat?ctc?tcc?tat?gcg?gac?agg?gct?gca 1489
His?Leu?Leu?Ala?Glu?Thr?Met?His?Leu?Ser?Tyr?Ala?Asp?Arg?Ala?Ala
320 325 330
tat?gcg?ggc?gat?ccg?gaa?ttt?gtc?gat?gtt?ccg?ctc?aga?ggg?ctg?ctc 1537
Tyr?Ala?Gly?Asp?Pro?Glu?Phe?Val?Asp?Val?Pro?Leu?Arg?Gly?Leu?Leu
335 340 345
gat?ccc?gat?tat?att?aag?gag?cgg?caa?aag?ctg?atc?agc?ctt?gat?tcg 1585
Asp?Pro?Asp?Tyr?Ile?Lys?Glu?Arg?Gln?Lys?Leu?Ile?Ser?Leu?Asp?Ser
350 355 360 365
atg?aac?cgc?gat?gtg?aaa?gag?ggc?gat?ccg?tgg?aaa?tat?gag?gaa?ggc 1633
Met?Asn?Arg?Asp?Val?Lys?Glu?Gly?Asp?Pro?Trp?Lys?Tyr?Glu?Glu?Gly
370 375 380
gag?ccg?aat?tat?gag?atc?gtg?cct?cag?cct?gaa?gat?aaa?acg?atc?ggg 1681
Glu?Pro?Asn?Tyr?Glu?Ile?Val?Pro?Gln?Pro?Glu?Asp?Lys?Thr?Ile?Gly
385 390 395
gag?acg?acg?cat?ttt?acc?gtc?acg?gat?cag?tgg?ggc?aat?gtt?gtc?tct 1729
Glu?Thr?Thr?His?Phe?Thr?Val?Thr?Asp?Gln?Trp?Gly?Asn?Val?Val?Ser
400 405 410
tat?acg?aca?acg?att?gaa?cag?ctt?ttt?gga?acg?ggg?att?tta?gtg?ccc 1777
Tyr?Thr?Thr?Thr?Ile?Glu?Gln?Leu?Phe?Gly?Thr?Gly?Ile?Leu?Val?Pro
415 420 425
ggg?tac?gga?ctg?ttt?ttg?aac?aat?gaa?ctg?acc?gat?ttt?gat?gcg?att 1825
Gly?Tyr?Gly?Leu?Phe?Leu?Asn?Asn?Glu?Leu?Thr?Asp?Phe?Asp?Ala?Ile
430 435 440 445
cca?ggc?ggc?gcc?aac?gaa?gtg?cag?ccg?aat?aaa?agg?ccg?ctt?tca?agc 1873
Pro?Gly?Gly?Ala?Asn?Glu?Val?Gln?Pro?Asn?Lys?Arg?Pro?Leu?Ser?Ser
450 455 460
atg?acg?ccg?acg?atc?gta?ttc?aaa?gat?gaa?aaa?ccg?gtg?ctg?act?gtc 1921
Met?Thr?Pro?Thr?Ile?Val?Phe?Lys?Asp?Glu?Lys?Pro?Val?Leu?Thr?Val
465 470 475
ggt?tcc?cct?ggc?gga?acg?acg?atc?att?gct?tcc?gtt?ttc?cag?acg?atc 1969
Gly?Ser?Pro?Gly?Gly?Thr?Thr?Ile?Ile?Ala?Ser?Val?Phe?Gln?Thr?Ile
480 485 490
ttg?aat?tat?ttt?gag?tac?ggc?atg?agc?ctt?cag?gat?gcg?att?gaa?gag 2017
Leu?Asn?Tyr?Phe?Glu?Tyr?Gly?Met?Ser?Leu?Gln?Asp?Ala?Ile?Glu?Glu
495 500 505
ccg?agg?atc?tat?acg?aac?agc?ctg?act?tcg?tac?cgc?tat?gaa?tcc?gga 2065
Pro?Arg?Ile?Tyr?Thr?Asn?Ser?Leu?Thr?Ser?Tyr?Arg?Tyr?Glu?Ser?Gly
510 515 520 525
atg?ccg?gag?gat?gtc?aga?cgg?aag?ctc?aat?gac?ttc?ggc?cac?aaa?ttc 2113
Met?Pro?Glu?Asp?Val?Arg?Arg?Lys?Leu?Asn?Asp?Phe?Gly?His?Lys?Phe
530 535 540
ggc?tcg?aat?ccg?gtt?gat?atc?ggc?aat?gtc?caa?agc?att?ttc?att?gac 2161
Gly?Ser?Asn?Pro?Val?Asp?Ile?Gly?Asn?Val?Gln?Ser?Ile?Phe?Ile?Asp
545 550 555
cgg?gag?aac?aaa?acg?ttt?atg?ggg?gtt?gcc?gat?tca?agc?aga?aac?gga 2209
Arg?Glu?Asn?Lys?Thr?Phe?Met?Gly?Val?Ala?Asp?Ser?Ser?Arg?Asn?Gly
560 565 570
acg?gcg?gtt?ggg?gtc?aac?att?aag?aca?tcg?gct?aaa?tagcggagca 2255
Thr?Ala?Val?Gly?Val?Asn?Ile?Lys?Thr?Ser?Ala?Lys
575 580 585
aggcttggtc?taaagtattt?agatcaagcc?ttcttccgtc?agcggggaaa?agaggatttt 2315
tttcgaaaga?atagaaatat?attttttgaa?agcgtttacc?aatttataaa?ggaggggtac 2375
catgattcac?acgttggcaa?atggatttta?ccgcttttgc?gagtgggtga?tgcggcttgc 2435
ctatttgaat?ctgctgtgga?tcggctttac?gctggcggga?gcggtcatct?tcggtttagc 2495
gccggcgacc?gccgcgatgt?tcgctgtgac?tagacagtgg?acgctgggaa?aaacggatgt 2555
cccggtgttt?cagacgtttt?ttcgggcgtt?taaaaagaaa?tgggccaaat?caagcgttct 2615
cggtttgata?ttgggtctgg?tcggcttatt?gctgtttgtt?gattttcaga?ttgcggccgt 2675
ctattttcac?gatcagccag?tgtttttaag?cctgttcatc?agtttgttta?tcatttatgc 2735
catcatcctc?t 2746
<210>12
<211>585
<212>PRT
＜213〉Bacillus licheniformis
<400>12
Met?Arg?Arg?Leu?Ala?Phe?Leu?Val?Val?Ala?Phe?Cys?Leu?Ala?Val?Gly
1 5 10 15
Cys?Phe?Phe?Ser?Pro?Val?Ser?Lys?Ala?Glu?Gly?Val?Met?Ser?Gly?Gly
20 25 30
Asp?Gly?Asp?Lys?Val?Ala?Val?Gly?Lys?Asp?Gly?Met?Val?Ala?Thr?Ala
35 40 45
His?Pro?Leu?Ala?Ser?Lys?Ile?Gly?Ala?Glu?Val?Leu?Lys?Lys?Gly?Gly
50 55 60
Asn?Ala?Ile?Asp?Ala?Ala?Ile?Ala?Ile?Gln?Tyr?Ala?Leu?Asn?Val?Thr
65 70 75 80
Glu?Pro?Met?Met?Ser?Gly?Ile?Gly?Gly?Gly?Gly?Phe?Met?Met?Val?Tyr
85 90 95
Asp?Gly?Glu?Thr?Lys?Glu?Thr?Ser?Ile?Ile?Asn?Ser?Arg?Glu?Arg?Ala
100 105 110
Pro?Glu?Gly?Ala?Lys?Pro?Asp?Met?Phe?Leu?Asp?Glu?Asp?Gly?Lys?Val
115 120 125
Ile?Pro?Phe?Ser?Glu?Arg?Ser?Arg?His?Gly?Asn?Ala?Val?Gly?Val?Pro
130 135 140
Gly?Thr?Leu?Lys?Gly?Leu?Glu?Ala?Ala?His?Lys?Lys?Trp?Gly?Thr?Lys
145 150 155 160
Lys?Met?Glu?Asp?Leu?Ile?Ser?Pro?Ser?Ile?Lys?Leu?Ala?Glu?Glu?Gly
165 170 175
Phe?Pro?Ile?Asp?Ser?Val?Leu?Ala?Asp?Ala?Ile?Lys?Asp?His?Gln?Asp
180 185 190
Lys?Leu?Ser?Lys?Thr?Ala?Ala?Lys?Asp?Ile?Phe?Leu?Pro?Asp?Gly?Glu
195 200 205
Pro?Leu?Lys?Glu?Gly?Asp?Ile?Leu?Val?Gln?Lys?Asp?Leu?Ala?Lys?Thr
210 215 220
Phe?Lys?Leu?Ile?Arg?Lys?Glu?Gly?Ser?Lys?Ala?Phe?Tyr?Asp?Gly?Glu
225 230 235 240
Ile?Gly?Arg?Ala?Ile?Ala?Asp?Val?Val?Gln?Asp?Phe?Gly?Gly?Ser?Met
245 250 255
Thr?Pro?Asp?Asp?Leu?Ser?Arg?Tyr?Glu?Val?Thr?Thr?Asp?Lys?Pro?Ile
260 265 270
Trp?Gly?Glu?Tyr?His?Gly?Tyr?Asp?Ile?Ala?Ser?Met?Pro?Pro?Pro?Ser
275 280 285
Ser?Gly?Gly?Val?Phe?Met?Leu?Gln?Met?Leu?Lys?Leu?Ile?Asp?Asp?Phe
290 295 300
His?Leu?Ser?Gln?Tyr?Asp?Pro?Lys?Ser?Phe?Glu?Lys?Tyr?His?Leu?Leu
305 310 315 320
Ala?Glu?Thr?Met?His?Leu?Ser?Tyr?Ala?Asp?Arg?Ala?Ala?Tyr?Ala?Gly
325 330 335
Asp?Pro?Glu?Phe?Val?Asp?Val?Pro?Leu?Arg?Gly?Leu?Leu?Asp?Pro?Asp
340 345 350
Tyr?Ile?Lys?Glu?Arg?Gln?Lys?Leu?Ile?Ser?Leu?Asp?Ser?Met?Asn?Arg
355 360 365
Asp?Val?Lys?Glu?Gly?Asp?Pro?Trp?Lys?Tyr?Glu?Glu?Gly?Glu?Pro?Asn
370 375 380
Tyr?Glu?Ile?Val?Pro?Gln?Pro?Glu?Asp?Lys?Thr?Ile?Gly?Glu?Thr?Thr
385 390 395 400
His?Phe?Thr?Val?Thr?Asp?Gln?Trp?Gly?Asn?Val?Val?Ser?Tyr?Thr?Thr
405 410 415
Thr?Ile?Glu?Gln?Leu?Phe?Gly?Thr?Gly?Ile?Leu?Val?Pro?Gly?Tyr?Gly
420 425 430
Leu?Phe?Leu?Asn?Asn?Glu?Leu?Thr?Asp?Phe?Asp?Ala?Ile?Pro?Gly?Gly
435 440 445
Ala?Asn?Glu?Val?Gln?Pro?Asn?Lys?Arg?Pro?Leu?Ser?Ser?Met?Thr?Pro
450 455 460
Thr?Ile?Val?Phe?Lys?Asp?Glu?Lys?Pro?Val?Leu?Thr?Val?Gly?Ser?Pro
465 470 475 480
Gly?Gly?Thr?Thr?Ile?Ile?Ala?Ser?Val?Phe?Gln?Thr?Ile?Leu?Asn?Tyr
485 490 495
Phe?Glu?Tyr?Gly?Met?Ser?Leu?Gln?Asp?Ala?Ile?Glu?Glu?Pro?Arg?Ile
500 505 510
Tyr?Thr?Asn?Ser?Leu?Thr?Ser?Tyr?Arg?Tyr?Glu?Ser?Gly?Met?Pro?Glu
515 520 525
Asp?Val?Arg?Arg?Lys?Leu?Asn?Asp?Phe?Gly?His?Lys?Phe?Gly?Ser?Asn
530 535 540
Pro?Val?Asp?Ile?Gly?Asn?Val?Gln?Ser?Ile?Phe?Ile?Asp?Arg?Glu?Asn
545 550 555 560
Lys?Thr?Phe?Met?Gly?Val?Ala?Asp?Ser?Ser?Arg?Asn?Gly?Thr?Ala?Val
565 570 575
Gly?Val?Asn?Ile?Lys?Thr?Ser?Ala?Lys
580 585
<210>13
<211>2314
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(493)..(1818)
<400>13
ctgaaatcta?tcaaggcaga?cgctttaaag?tttatcgcgg?catgggttct?gtagctgcga 60
tggaaaaagg?aagcaaagac?cgctacttcc?aagaggaaaa?caaaaaattc?gttcctgaag 120
gcattgaagg?ccgtacgcct?tataaaggcc?ctgttgctga?taccgtttat?cagcttgtcg 180
gcggcttgaa?atccggcatg?ggctactgcg?gaacgaaaga?tttgcacgca?ttgagggaag 240
atgcgcagtt?catccgcatg?acaggagcag?gcctgcggga?aagccatcct?cacgatgtgc 300
aaattaccaa?agaatcgcct?aactatacca?tttcctaatc?aaatatgaca?aaaattaaac 360
cgtagacaga?gtgatactct?gtctattttt?ttttgcttca?ttatgataaa?atttataatg 420
ttgtgtttga?aaaatgtctt?aaaaggcttt?gatatagatt?attagtctga?tgaaaagcgg 480
aggtatcacg?at?ttg?aaa?agc?aag?aga?tta?aag?cag?ctg?atc?atg?tta?atc 531
Leu?Lys?Ser?Lys?Arg?Leu?Lys?Gln?Leu?Ile?Met?Leu?Ile
1 5 10
gtt?gct?ttt?gcc?gtg?act?gta?ggt?gca?ttt?tct?cct?atg?tca?act?gca 579
Val?Ala?Phe?Ala?Val?Thr?Val?Gly?Ala?Phe?Ser?Pro?Met?Ser?Thr?Ala
15 20 25
aaa?gct?gca?aat?gat?cca?atc?aat?gta?gac?gcg?aaa?gca?gca?arc?ctg 627
Lys?Ala?Ala?Asn?Asp?Pro?Ile?Asn?Val?Asp?Ala?Lys?Ala?Ala?Ile?Leu
30 35 40 45
gtg?gaa?gct?tca?tcg?ggg?aaa?att?ttg?tac?agt?aaa?aat?gcc?gac?cag 675
Val?Glu?Ala?Ser?Ser?Gly?Lys?Ile?Leu?Tyr?Ser?Lys?Asn?Ala?Asp?Gln
50 55 60
cgt?ctt?cct?gtt?gcc?agc?atg?gca?aaa?atg?atg?acg?gag?tat?ctt?ctg 723
Arg?Leu?Pro?Val?Ala?Ser?Met?Ala?Lys?Met?Met?Thr?Glu?Tyr?Leu?Leu
65 70 75
ttg?gaa?gcg?att?cac?gaa?ggc?aaa?gtg?aaa?tgg?gat?cag?aag?tac?acg 771
Leu?Glu?Ala?Ile?His?Glu?Gly?Lys?Val?Lys?Trp?Asp?Gln?Lys?Tyr?Thr
80 85 90
cct?gac?gat?tat?gta?tac?gag?att?tcc?caa?gat?cgt?tca?cta?tcc?aat 819
Pro?Asp?Asp?Tyr?Val?Tyr?Glu?Ile?Ser?Gln?Asp?Arg?Ser?Leu?Ser?Asn
95 100 105
gtt?cct?ctt?cgt?aaa?gac?gga?tca?tac?act?gta?aaa?gag?ctt?tat?cag 867
Val?Pro?Leu?Arg?Lys?Asp?Gly?Ser?Tyr?Thr?Val?Lys?Glu?Leu?Tyr?Gln
110 115 120 125
gcg?act?gca?att?tac?tct?gca?aac?gcg?gcg?gct?att?gca?ctt?tct?gaa 915
Ala?Thr?Ala?Ile?Tyr?Ser?Ala?Asn?Ala?Ala?Ala?Ile?Ala?Leu?Ser?Glu
130 135 140
att?gtt?gcc?ggt?tct?gaa?tcg?aag?ttt?gta?gag?tta?atg?aac?aaa?aaa 963
Ile?Val?Ala?Gly?Ser?Glu?Ser?Lys?Phe?Val?Glu?Leu?Met?Asn?Lys?Lys
145 150 155
gcc?aaa?gag?ctt?gga?atg?aaa?gat?ttc?aaa?ttt?gtg?aat?gcc?aca?ggt 1011
Ala?Lys?Glu?Leu?Gly?Met?Lys?Asp?Phe?Lys?Phe?Val?Asn?Ala?Thr?Gly
160 165 170
ttg?gaa?aac?aag?gat?ctt?cac?ggc?aaa?cat?ccg?agc?gga?aca?aat?gcc 1059
Leu?Glu?Asn?Lys?Asp?Leu?His?Gly?Lys?His?Pro?Ser?Gly?Thr?Asn?Ala
175 180 185
aat?gaa?gaa?aat?gaa?gtg?tct?gcg?aga?gac?atg?gcg?ctc?ctt?gcc?gat 1107
Asn?Glu?Glu?Asn?Glu?Val?Ser?Ala?Arg?Asp?Met?Ala?Leu?Leu?Ala?Asp
190 195 200 205
cat?tta?gtg?agc?gat?tat?cca?gag?atc?ctc?gat?acg?gcg?agc?att?gcc 1155
His?Leu?Val?Ser?Asp?Tyr?Pro?Glu?Ile?Leu?Asp?Thr?Ala?Ser?Ile?Ala
210 215 220
aaa?acg?aag?ttc?aga?aaa?ggc?aca?gac?gat?gaa?atg?gat?atg?ccg?aac 1203
Lys?Thr?Lys?Phe?Arg?Lys?Gly?Thr?Asp?Asp?Glu?Met?Asp?Met?Pro?Asn
225 230 235
tgg?aac?ttt?atg?ctg?aaa?ggc?ctt?gtt?cag?caa?tat?gaa?ggt?gtg?gac 1251
Trp?Asn?Phe?Met?Leu?Lys?Gly?Leu?Val?Gln?Gln?Tyr?Glu?Gly?Val?Asp
240 245 250
ggg?ctg?aaa?acc?ggg?tca?acc?gac?tca?gcc?gga?tca?tgc?ttt?acg?gct 1299
Gly?Leu?Lys?Thr?Gly?Ser?Thr?Asp?Ser?Ala?Gly?Ser?Cys?Phe?Thr?Ala
255 260 265
act?gct?gaa?aga?aac?ggc?atg?aga?gtg?att?tcc?gtc?gtt?ctg?aat?gcg 1347
Thr?Ala?Glu?Arg?Asn?Gly?Met?Arg?Val?Ile?Ser?Val?Val?Leu?Asn?Ala
270 275 280 285
aaa?ggc?gac?ctt?cat?acg?gcg?cga?ttt?aaa?gaa?acg?aaa?aaa?atg?ctc 1395
Lys?Gly?Asp?Leu?His?Thr?Ala?Arg?Phe?Lys?Glu?Thr?Lys?Lys?Met?Leu
290 295 300
gat?tat?gca?ttt?gaa?aac?ttc?act?atg?aaa?gag?ttt?tac?gca?aaa?ggc 1443
Asp?Tyr?Ala?Phe?Glu?Asn?Phe?Thr?Met?Lys?Glu?Phe?Tyr?Ala?Lys?Gly
305 310 315
gca?caa?gtg?aaa?ggc?aac?gaa?aca?gtc?aca?gtc?gac?aaa?ggg?aaa?gaa 1491
Ala?Gln?Val?Lys?Gly?Asn?Glu?Thr?Val?Thr?Val?Asp?Lys?Gly?Lys?Glu
320 325 330
act?gaa?gtc?ggc?atc?gtc?aca?gaa?aaa?gcc?ttt?tca?atg?cct?gtg?aag 1539
Thr?Glu?Val?Gly?Ile?Val?Thr?Glu?Lys?Ala?Phe?Ser?Met?Pro?Val?Lys
335 340 345
aac?ggt?gaa?gaa?aag?aat?tac?aaa?gcg?aaa?gtg?acg?ctg?aac?aaa?gac 1587
Asn?Gly?Glu?Glu?Lys?Asn?Tyr?Lys?Ala?Lys?Val?Thr?Leu?Asn?Lys?Asp
350 355 360 365
gag?ctg?acc?gca?cct?gtt?aaa?aaa?ggt?gag?aaa?gtc?ggt?acc?ttg?acg 1635
Glu?Leu?Thr?Ala?Pro?Val?Lys?Lys?Gly?Glu?Lys?Val?Gly?Thr?Leu?Thr
370 375 380
gcc?tcc?tat?aag?gga?gaa?gaa?aag?gac?tac?gga?ttt?ctc?ggc?agc?gat 1683
Ala?Ser?Tyr?Lys?Gly?Glu?Glu?Lys?Asp?Tyr?GIy?Phe?Leu?Gly?Ser?Asp
385 390 395
gta?tca?ggt?gta?aac?ctg?gtg?aca?aaa?gaa?gat?gat?gag?aaa?gca?aac 1731
Val?Ser?Gly?Val?Asn?Leu?Val?Thr?Lys?Glu?Asp?Asp?Glu?Lys?Ala?Ash
400 405 410
tgg?ttt?att?tta?acg?atg?aga?agc?atc?ggc?ggc?ttt?ttt?gcc?ggt?ata 1779
Trp?Phe?Ile?Leu?Thr?Met?Arg?Ser?Ile?Gly?Gly?Phe?Phe?Ala?Gly?Ile
415 420 425
tgg?aac?agc?att?gtt?gaa?atg?gta?acc?ggc?tgg?ttt?taa?aaaataaaga 1828
Trp?Asn?Ser?Ile?Val?Glu?Met?Val?Thr?Gly?Trp?Phe
430 435 440
gctctatttg?atagggctct?ttattgtcat?attcagatag?aattaccatt?tacaatccta 1888
acgatttgtt?attaaaatag?aaataatctt?cgtgtttttc?atatgatttt?gtggaattat 1948
aggatacaat?aggattaaat?ctgacaattc?aatctttgat?tagggggatc?cttgaaatgg 2008
ctcaaacagg?tactgatcgc?gtaaaacgcg?gtatggcaga?aatgcagaag?ggcggcgtca 2068
ttatggacgt?tgtcaatgct?gaacaagcaa?aaatcgcaga?agaagcagga?gccgtcgcag 2128
tcatggcgct?ggagcgcgtt?ccggctgata?tccgtgcggc?aggcggagtt?gcccgcatgg 2188
cagatccgac?tattgtcgaa?gaagtgatga?acgctgtttc?aatcccggtt?atggcaaaag 2248
cacgcatcgg?gcatatcgtt?gaagcgcgtg?ttcttgaagc?gcttggcgtt?gactatattg 2308
atgaaa 2314
<210>14
<211>441
<212>PRT
＜213〉Bacillus licheniformis
<400>14
Leu?Lys?Ser?Lys?Arg?Leu?Lys?Gln?Leu?Ile?Met?Leu?Ile?Val?Ala?Phe
1 5 10 15
Ala?Val?Thr?Val?Gly?Ala?Phe?Set?Pro?Met?Ser?Thr?Ala?Lys?Ala?Ala
20 25 30
Asn?Asp?Pro?Ile?Asn?Val?Asp?Ala?Lys?Ala?Ala?Ile?Leu?Val?Glu?Ala
35 40 45
Ser?Ser?Gly?Lys?Ile?Leu?Tyr?Ser?Lys?Asn?Ala?Asp?Gln?Arg?Leu?Pro
50 55 60
Val?Ala?Ser?Met?Ala?Lys?Met?Met?Thr?Glu?Tyr?Leu?Leu?Leu?Glu?Ala
65 70 75 80
Ile?His?Glu?Gly?Lys?Val?Lys?Trp?Asp?Gln?Lys?Tyr?Thr?Pro?Asp?Asp
85 90 95
Tyr?Val?Tyr?Glu?Ile?Ser?Gln?Asp?Arg?Ser?Leu?Ser?Asn?Val?Pro?Leu
100 105 110
Arg?Lys?Asp?Gly?Ser?Tyr?Thr?Val?Lys?Glu?Leu?Tyr?Gln?Ala?Thr?Ala
115 120 125
Ile?Tyr?Ser?Ala?Asn?Ala?Ala?Ala?Ile?Ala?Leu?Ser?Glu?Ile?Val?Ala
130 135 140
Gly?Ser?Glu?Ser?Lys?Phe?Val?Glu?Leu?Met?Asn?Lys?Lys?Ala?Lys?Glu
145 150 155 160
Leu?Gly?Met?Lys?Asp?Phe?Lys?Phe?Val?Asn?Ala?Thr?Gly?Leu?Glu?Asn
165 170 175
Lys?Asp?Leu?His?Gly?Lys?His?Pro?Ser?Gly?Thr?Asn?Ala?Asn?Glu?Glu
180 185 190
Asn?Glu?Val?Ser?Ala?Arg?Asp?Met?Ala?Leu?Leu?Ala?Asp?His?Leu?Val
195 200 205
Ser?Asp?Tyr?Pro?Glu?Ile?Leu?Asp?Thr?Ala?Ser?Ile?Ala?Lys?Thr?Lys
210 215 220
Phe?Arg?Lys?Gly?Thr?Asp?Asp?Glu?Met?Asp?Met?Pro?Asn?Trp?Asn?Phe
225 230 235 240
Met?Leu?Lys?Gly?Leu?Val?Gln?Gln?Tyr?Glu?Gly?Val?Asp?Gly?Leu?Lys
245 250 255
Thr?Gly?Ser?Thr?Asp?Ser?Ala?Gly?Ser?Cys?Phe?Thr?Ala?Thr?Ala?Glu
260 265 270
Arg?Asn?Gly?Met?Arg?Val?Ile?Ser?Val?Val?Leu?Asn?Ala?Lys?Gly?Asp
275 280 285
Leu?His?Thr?Ala?Arg?Phe?Lys?Glu?Thr?Lys?Lys?Met?Leu?Asp?Tyr?Ala
290 295 300
Phe?Glu?Asn?Phe?Thr?Met?Lys?Glu?Phe?Tyr?Ala?Lys?Gly?Ala?Gln?Val
305 310 315 320
Lys?Gly?Asn?Glu?Thr?Val?Thr?Val?Asp?Lys?Gly?Lys?Glu?Thr?Glu?Val
325 330 335
Gly?Ile?Val?Thr?Glu?Lys?Ala?Phe?Ser?Met?Pro?Val?Lys?Asn?Gly?Glu
340 345 350
Glu?Lys?Asn?Tyr?Lys?Ala?Lys?Val?Thr?Leu?Asn?Lys?Asp?Glu?Leu?Thr
355 360 365
Ala?Pro?Val?Lys?Lys?Gly?Glu?Lys?Val?Gly?Thr?Leu?Thr?Ala?Ser?Tyr
370 375 380
Lys?Gly?Glu?Glu?Lys?Asp?Tyr?Gly?Phe?Leu?Gly?Ser?Asp?Val?Ser?Gly
385 390 395 400
Val?Asn?Leu?Val?Thr?Lys?Glu?Asp?Asp?Glu?Lys?Ala?Asn?Trp?Phe?Ile
405 410 415
Leu?Thr?Met?Arg?Ser?Ile?Gly?Gly?Phe?Phe?Ala?Gly?Ile?Trp?Asn?Ser
420 425 430
Ile?Val?Glu?Met?Val?Thr?Gly?Trp?Phe
435 440
<210>15
<211>2431
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(519)..(1958)
<400>15
agaagcttga?cgtcattgtt?gccagcaatt?tattcggcga?tattttgacc?gatatcgggg 60
cggcgattat?gggaagcata?ggtgtggcgc?cggccgccaa?cattaatgta?aacggcaaat 120
atccatctat?gtttgagcct?gtgcacggct?ctgctccgga?catcatctga?tagcgagtca 180
tcatcggaaa?aggaatcgcc?aatccgatcg?gccaaatttg?gacggcaaaa?atgatgctcg 240
atcatttcgg?ggaagaagaa?ctcggtgcaa?gattgcttga?cgtgattgaa?gacgttctaa 300
agagcggcta?cttaacgcct?gatatcggcg?gttcaagtac?gacggatgat?gtgacagaag 360
aaatcatcaa?gcgcgtgagg?accatataag?cgaaagctgc?cagcactccg?gcagcttttt 420
ttgctgtgga?atggaaaatc?gattacatga?tacgggcggt?tttggcgcat?actacatcaa 480
gcaacaaaac?atagtgcagg?cagaagagga?ggtatagg?atg?aat?cag?aag?att?gtg 536
Met?Asn?Gln?Lys?Ile?Val
1 5
tct?gtg?ctc?atc?att?gcg?ctc?agc?ctg?tta?tgc?ggc?ttg?gta?aca?ggc 584
Ser?Val?Leu?Ile?Ile?Ala?Leu?Ser?Leu?Leu?Cys?Gly?Leu?Val?Thr?Gly
10 15 20
gcc?ggc?ccg?att?ccg?tta?cat?gat?gtc?gtt?tca?aaa?gca?tca?gcg?gct 632
Ala?Gly?Pro?Ile?Pro?Leu?His?Asp?Val?Val?Ser?Lys?Ala?Ser?Ala?Ala
25 30 35
gaa?gaa?gct?gca?gcc?aaa?agc?acc?gct?aga?aac?gaa?ttg?gag?aaa?att 680
Glu?Glu?Ala?Ala?Ala?Lys?Ser?Thr?Ala?Arg?Asn?Glu?Leu?Glu?Lys?Ile
40 45 50
gag?aaa?gca?tat?gac?ctg?att?tca?aat?gaa?tat?gtg?gag?cag?gtc?gac 728
Glu?Lys?Ala?Tyr?Asp?Leu?Ile?Ser?Asn?Glu?Tyr?Val?Glu?Gln?Val?Asp
55 60 65 70
agg?gaa?aaa?ctg?ttg?gaa?gga?gcc?ata?cag?ggc?atg?ctt?tca?aca?ttg 776
Arg?Glu?Lys?Leu?Leu?Glu?Gly?Ala?Ile?Gln?Gly?Met?Leu?Ser?Thr?Leu
75 80 85
aat?gac?cca?tac?tcg?gtc?tat?atg?gat?aaa?cag?acg?gca?aag?cgg?ttt 824
Asn?Asp?Pro?Tyr?Ser?Val?Tyr?Met?Asp?Lys?Gln?Thr?Ala?Lys?Arg?Phe
90 95 100
tca?gat?tcc?ctt?gat?tcc?tct?ttt?gaa?gga?atc?ggt?gca?gaa?atc?gga 872
Ser?Asp?Ser?Leu?Asp?Ser?Ser?Phe?Glu?Gly?Ile?Gly?Ala?Glu?Ile?Gly
105 110 115
atg?gaa?gac?aga?aaa?atc?att?atc?gtt?tct?cct?ttt?aaa?cag?tct?ccg 920
Met?Glu?Asp?Arg?Lys?Ile?Ile?Ile?Val?Ser?Pro?Phe?Lys?Gln?Ser?Pro
120 125 130
gca?gag?aaa?gcc?gga?tta?aag?ccg?aac?gat?gaa?atc?att?agc?atc?gac 968
Ala?Glu?Lys?Ala?Gly?Leu?Lys?Pro?Asn?Asp?Glu?Ile?Ile?Ser?Ile?Asp
135 140 145 150
ggc?gat?tcc?atg?agc?gga?atg?gat?ttg?aat?gac?gcc?gta?tta?aag?atc 1016
Gly?Asp?Ser?Met?Ser?Gly?Met?Asp?Leu?Asn?Asp?Ala?Val?Leu?Lys?Ile
155 160 165
aga?ggc?aaa?aag?gga?tcg?acg?gtt?aca?ctg?aaa?gtt?cac?cga?ccc?gga 1064
Arg?Gly?Lys?Lys?Gly?Ser?Thr?Val?Thr?Leu?Lys?Val?His?Arg?Pro?Gly
170 175 180
atg?aaa?gac?cag?ctg?aca?ttt?acg?att?aag?cgc?gat?gaa?atc?ccg?ctg 1112
Met?Lys?Asp?Gln?Leu?Thr?Phe?Thr?Ile?Lys?Arg?Asp?Glu?Ile?Pro?Leu
185 190 195
gaa?acg?gtg?ttt?gct?tcc?ata?aag?aaa?gta?cag?gat?aaa?cct?gtc?ggc 1160
Glu?Thr?Val?Phe?Ala?Ser?Ile?Lys?Lys?Val?Gln?Asp?Lys?Pro?Val?Gly
200 205 210
tat?atc?gca?ata?tct?tcc?ttt?tcc?gaa?cat?acg?gca?aaa?gat?ttt?act 1208
Tyr?Ile?Ala?Ile?Ser?Ser?Phe?Ser?Glu?His?Thr?Ala?Lys?Asp?Phe?Thr
215 220 225 230
gct?gaa?ctg?aaa?aag?ctt?gag?aaa?aag?gga?ata?aaa?ggg?ctc?gtt?ttg 1256
Ala?Glu?Leu?Lys?Lys?Leu?Glu?Lys?Lys?Gly?Ile?Lys?Gly?Leu?Val?Leu
235 240 245
gat?gta?aga?gga?aat?ccg?ggc?ggg?tac?ttg?caa?agc?gtg?gaa?gac?att 1304
Asp?Val?Arg?Gly?Asn?Pro?Gly?Gly?Tyr?Leu?Gln?Ser?Val?Glu?Asp?Ile
250 255 260
tta?aaa?cat?ttt?gtg?aca?aaa?gat?cat?ccg?tat?ata?caa?atc?gcc?gaa 1352
Leu?Lys?His?Phe?Val?Thr?Lys?Asp?His?Pro?Tyr?Ile?Gln?Ile?Ala?Glu
265 270 275
aga?aac?gga?aat?aaa?aaa?caa?tac?ttc?tca?aaa?tta?aaa?gag?aaa?aaa 1400
Arg?Asn?Gly?Asn?Lys?Lys?Gln?Tyr?Phe?Ser?Lys?Leu?Lys?Glu?Lys?Lys
280 285 290
cca?tac?cct?gtg?agc?gtg?atc?acg?gac?aag?ggc?agc?gct?tcg?gct?tcg 1448
Pro?Tyr?Pro?Val?Ser?Val?Ile?Thr?Asp?Lys?Gly?Ser?Ala?Ser?Ala?Ser
295 300 305 310
gaa?att?ctt?gca?ggc?gca?tta?aaa?gaa?gcc?gag?ggc?tac?gac?gtg?gtt 1496
Glu?Ile?Leu?Ala?Gly?Ala?Leu?Lys?Glu?Ala?Glu?Gly?Tyr?Asp?Val?Val
315 320 325
ggc?gat?cct?tcc?ttt?gga?aag?gga?acc?gtc?cag?cag?gcg?gtg?ccg?atg 1544
Gly?Asp?Pro?Ser?Phe?Gly?Lys?Gly?Thr?Val?Gln?Gln?Ala?Val?Pro?Met
330 335 340
gga?gac?ggt?agc?aat?att?aag?ctg?acg?ctg?tac?aaa?tgg?ctg?aca?cct 1592
Gly?Asp?Gly?Ser?Asn?Ile?Lys?Leu?Thr?Leu?Tyr?Lys?Trp?Leu?Thr?Pro
345 350 355
aaa?gga?aat?tgg?atc?cat?aag?cag?ggc?att?cag?ccg?acc?gtt?cca?gtc 1640
Lys?Gly?Asn?Trp?Ile?His?Lys?Gln?Gly?Ile?Gln?Pro?Thr?Val?Pro?Val
360 365 370
acg?cag?cct?gca?tat?ttc?tcg?gcg?ggg?cct?tta?cag?ctg?aaa?gaa?ccc 1688
Thr?Gln?Pro?Ala?Tyr?Phe?Ser?Ala?Gly?Pro?Leu?Gln?Leu?Lys?Glu?Pro
375 380 385 390
ctc?aag?ccg?gat?atg?aac?aac?aat?gaa?att?aag?cgg?gcg?cag?ttc?ctg 1736
Leu?Lys?Pro?Asp?Met?Asn?Asn?Asn?Glu?Ile?Lys?Arg?Ala?Gln?Phe?Leu
395 400 405
ctg?aaa?gga?ctc?ggg?ttt?gtc?ccc?ggc?cgg?gaa?gac?ggc?tat?tac?aat 1784
Leu?Lys?Gly?Leu?Gly?Phe?Val?Pro?Gly?Arg?Glu?Asp?Gly?Tyr?Tyr?Asn
410 415 420
gaa?agc?aca?aaa?aaa?gcc?gtc?atg?gcg?ttt?cag?gca?gca?aac?aag?ctg 1832
Glu?Ser?Thr?Lys?Lys?Ala?Val?Met?Ala?Phe?Gln?Ala?Ala?Asn?Lys?Leu
425 430 435
aag?caa?aca?ggg?atc?att?gac?caa?aaa?acg?gca?aat?aca?atg?aac?ctg 1880
Lys?Gln?Thr?Gly?Ile?Ile?Asp?Gln?Lys?Thr?Ala?Asn?Thr?Met?Asn?Leu
440 445 450
cgg?att?gag?gag?aaa?aag?atg?gat?gaa?aag?aac?gat?ctt?cag?ctg?caa 1928
Arg?Ile?Glu?Glu?Lys?Lys?Met?Asp?Glu?Lys?Asn?Asp?Leu?Gln?Leu?Gln
455 460 465 470
gcc?gca?tta?aaa?gtg?ctg?ttt?aac?aaa?aag?tgagggcacg?ggatgtcctc 1978
Ala?Ala?Leu?Lys?Val?Leu?Phe?Asn?Lys?Lys
475 480
acttttttta?ttctcagaaa?gtaaattctc?aatcttttta?cctaaaattt?ttgctttttc 2038
ataaaaaaaa?cattcataat?gaatagaatt?gacccgataa?cataagtata?tgggagactt 2098
tataaggatg?taaagatgca?gtaaaaatgt?aacttttgca?agttgtataa?cataggaaaa 2158
ctttttaaaa?tagaaccaaa?gctttttcaa?gaattataat?gtgtgcattt?tttttgcgga 2218
gaatgttagc?gctgtttgcg?ttcttcgtct?ctgttggtgg?agtcgtctga?cgcttgcgct 2278
tttcttatat?ataaataata?aatgggggta?cacgattgaa?aagggcaagt?attgtgagag 2338
agaaaaaata?ctatgaatta?gtggagcagt?taaaagttcg?atcacaagac?gttacgtttt 2398
ccgctacaaa?ggcagtagga?ttgcttatgc?tgt 2431
<210>16
<211>480
<212>PRT
＜213〉Bacillus licheniformis
<400>16
Met?Asn?Gln?Lys?Ile?Val?Ser?Val?Leu?Ile?Ile?Ala?Leu?Ser?Leu?Leu
1 5 10 15
Cys?Gly?Leu?Val?Thr?Gly?Ala?Gly?Pro?Ile?Pro?Leu?His?Asp?Val?Val
20 25 30
Ser?Lys?Ala?Ser?Ala?Ala?Glu?Glu?Ala?Ala?Ala?Lys?Ser?Thr?Ala?Arg
35 40 45
Asn?Glu?Leu?Glu?Lys?Ile?Glu?Lys?Ala?Tyr?Asp?Leu?Ile?Ser?Asn?Glu
50 55 60
Tyr?Val?Glu?Gln?Val?Asp?Arg?Glu?Lys?Leu?Leu?Glu?Gly?Ala?Ile?Gln
65 70 75 80
Gly?Met?Leu?Ser?Thr?Leu?Asn?Asp?Pro?Tyr?Ser?Val?Tyr?Met?Asp?Lys
85 90 95
Gln?Thr?Ala?Lys?Arg?Phe?Ser?Asp?Ser?Leu?Asp?Ser?Ser?Phe?Glu?Gly
100 105 110
Ile?Gly?Ala?Glu?Ile?Gly?Met?Glu?Asp?Arg?Lys?Ile?Ile?Ile?Val?Ser
115 120 125
Pro?Phe?Lys?Gln?Ser?Pro?Ala?Glu?Lys?Ala?Gly?Leu?Lys?Pro?Asn?Asp
130 135 140
Glu?Ile?Ile?Ser?Ile?Asp?Gly?Asp?Ser?Met?Ser?Gly?Met?Asp?Leu?Asn
145 150 155 160
Asp?Ala?Val?Leu?Lys?Ile?Arg?Gly?Lys?Lys?Gly?Ser?Thr?Val?Thr?Leu
165 170 175
Lys?Val?His?Arg?Pro?Gly?Met?Lys?Asp?Gln?Leu?Thr?Phe?Thr?Ile?Lys
180 185 190
Arg?Asp?Glu?Ile?Pro?Leu?Glu?Thr?Val?Phe?Ala?Ser?Ile?Lys?Lys?Val
195 200 205
Gln?Asp?Lys?Pro?Val?Gly?Tyr?Ile?Ala?Ile?Ser?Ser?Phe?Ser?Glu?His
210 215 220
Thr?Ala?Lys?Asp?Phe?Thr?Ala?Glu?Leu?Lys?Lys?Leu?Glu?Lys?Lys?Gly
225 230 235 240
Ile?Lys?Gly?Leu?Val?Leu?Asp?Val?Arg?Gly?Asn?Pro?Gly?Gly?Tyr?Leu
245 250 255
Gln?Ser?Val?Glu?Asp?Ile?Leu?Lys?His?Phe?Val?Thr?Lys?Asp?His?Pro
260 265 270
Tyr?Ile?Gln?Ile?Ala?Glu?Arg?Asn?Gly?Asn?Lys?Lys?Gln?Tyr?Phe?Ser
275 280 285
Lys?Leu?Lys?Glu?Lys?Lys?Pro?Tyr?Pro?Val?Ser?Val?Ile?Thr?Asp?Lys
290 295 300
Gly?Ser?Ala?Ser?Ala?Ser?Glu?Ile?Leu?Ala?Gly?Ala?Leu?Lys?Glu?Ala
305 310 315 320
Glu?Gly?Tyr?Asp?Val?Val?Gly?Asp?Pro?Ser?Phe?Gty?Lys?Gly?Thr?Val
325 330 335
Gln?Gln?Ala?Val?Pro?Met?Gly?Asp?Gly?Ser?Asn?Ile?Lys?Leu?Thr?Leu
340 345 350
Tyr?Lys?Trp?Leu?Thr?Pro?Lys?Gly?Asn?Trp?Ile?His?Lys?Gln?Gly?Ile
355 360 365
Gln?Pro?Thr?Val?Pro?Val?Thr?Gln?Pro?Ala?Tyr?Phe?Ser?Ala?Gly?Pro
370 375 380
Leu?Gln?Leu?Lys?Glu?Pro?Leu?Lys?Pro?Asp?Met?Asn?Asn?Asn?Glu?Ile
385 390 395 400
Lys?Arg?Ala?Gln?Phe?Leu?Leu?Lys?Gly?Leu?Gly?Phe?Val?Pro?Gly?Arg
405 410 415
Glu?Asp?Gly?Tyr?Tyr?Asn?Glu?Ser?Thr?Lys?Lys?Ala?Val?Met?Ala?Phe
420 425 430
Gln?Ala?Ala?Asn?Lys?Leu?Lys?Gln?Thr?Gly?Ile?Ile?Asp?Gln?Lys?Thr
435 440 445
Ala?Asn?Thr?Met?Asn?Leu?Arg?Ile?Glu?Glu?Lys?Lys?Met?Asp?Glu?Lys
450 455 460
Asn?Asp?Leu?Gln?Leu?Gln?Ala?Ala?Leu?Lys?Val?Leu?Phe?Asn?Lys?Lys
465 470 475 480
<210>17
<211>2230
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1730)
<400>17
ctgtcctccg?gagctgagcg?gagacattgt?cgaccggggc?gtgattttga?ccggaggagg 60
cgcgcttttg?aacggcatca?aagaatggct?gtcacaggaa?atcgttgtgc?ccgtgcatgt 120
tgctgcaaat?ccgcttgaat?ctgtcgctat?cggaacgggt?cgttctctcg?aagtgatcga 180
taaattgcaa?aaagcggcta?aataattttt?gttttcgcgt?cttttttccc?ggcttctttc 240
atatcgtgag?gaatggagaa?acgataagga?ggaagaccat?gcttagagat?atgggaagac 300
gcctggccat?caccgttatt?ttaagcggta?ttattctcgg?ggggatgagc?atctcccttg 360
caaacatgcc?aagctcgccc?cccggccaaa?cggccaaaat?caaccgttaa?aaaagaagca 420
gcctgaaccg?ggctgcttct?ttttttcttt?tcaatcaaat?atttgatatg?atgttatgga 480
caataaagga?ggttttgcca?atg?aat?tcg?ttt?act?gat?aaa?ctg?agc?aaa?tat 533
Met?Asn?Ser?Phe?Thr?Asp?Lys?Leu?Ser?Lys?Tyr
1 5 10
gca?aag?ctt?gtg?gcc?gaa?gtc?ggc?gta?aac?gtt?caa?aaa?gga?cag?caa 581
Ala?Lys?Leu?Val?Ala?Glu?Val?Gly?Val?Asn?Val?Gln?Lys?Gly?Gln?Gln
15 20 25
gtc?gtc?atc?aac?gcg?tca?aca?gaa?gta?cgc?gat?ttt?gca?cgg?ctc?ctc 629
Val?Val?Ile?Asn?Ala?Ser?Thr?Glu?Val?Arg?Asp?Phe?Ala?Arg?Leu?Leu
30 35 40
gtc?aaa?agc?gcc?tat?caa?aga?ggg?gcg?aaa?aac?gtg?acg?gtc?cgc?tgg 677
Val?Lys?Ser?Ala?Tyr?Gln?Arg?Gly?Ala?Lys?Asn?Val?Thr?Val?Arg?Trp
45 50 55
cag?gat?gat?gag?gtc?aca?agg?ctg?aaa?tac?gag?ctg?gct?cct?gcg?gaa 725
Gln?Asp?Asp?Glu?Val?Thr?Arg?Leu?Lys?Tyr?Glu?Leu?Ala?Pro?Ala?Glu
60 65 70 75
gcc?ttt?gaa?gag?ttc?cct?gaa?tgg?gaa?gca?aag?ggg?ctt?gag?acg?ctc 773
Ala?Phe?Glu?Glu?Phe?Pro?Glu?Trp?Glu?Ala?Lys?Gly?Leu?Glu?Thr?Leu
80 85 90
gcc?aaa?gaa?ggc?gct?gct?ttt?att?tcc?att?gtc?tct?tca?agt?cct?gat 821
Ala?Lys?Glu?Gly?Ala?Ala?Phe?Ile?Ser?Ile?Val?Ser?Ser?Ser?Pro?Asp
95 100 105
ctt?ttg?aaa?ggc?atc?gat?tcc?aaa?aaa?atc?tcc?gca?cag?cag?aaa?gcc 869
Leu?Leu?Lys?Gly?Ile?Asp?Ser?Lys?Lys?Ile?Ser?Ala?Gln?Gln?Lys?Ala
110 115 120
gca?gga?aaa?gcg?atg?cat?aca?tac?agg?caa?atg?att?caa?tcg?gat?aaa 917
Ala?Gly?Lys?Ala?Met?His?Thr?Tyr?Arg?Gln?Met?Ile?Gln?Ser?Asp?Lys
125 130 135
gtc?agc?tgg?act?gtc?gtc?gca?gcg?cct?tcg?ccg?gcc?tgg?gcg?aaa?aaa 965
Val?Ser?Trp?Thr?Val?Val?Ala?Ala?Pro?Ser?Pro?Ala?Trp?Ala?Lys?Lys
140 145 150 155
gta?ttc?cct?gag?gca?gat?gaa?gag?aca?gcc?gtg?caa?aag?cta?tgg?aat 1013
Val?Phe?Pro?Glu?Ala?Asp?Glu?Glu?Thr?Ala?Val?Gln?Lys?Leu?Trp?Asn
160 165 170
gag?att?ttc?aaa?acg?acc?cgc?gtt?gac?cgt?gaa?gat?ccc?gtt?caa?gcg 1061
Glu?Ile?Phe?Lys?Thr?Thr?Arg?Val?Asp?Arg?Glu?Asp?Pro?Val?Gln?Ala
175 180 185
tgg?aaa?gat?cac?gat?caa?acc?ttg?cgc?gat?aaa?gta?agc?gtc?tta?aac 1109
Trp?Lys?Asp?His?Asp?Gln?Thr?Leu?Arg?Asp?Lys?Val?Ser?Val?Leu?Asn
190 195 200
gac?aag?cat?tat?cac?gcc?ctt?cat?tac?caa?gca?aag?gga?aca?gat?ttg 1157
Asp?Lys?His?Tyr?His?Ala?Leu?His?Tyr?Gln?Ala?Lys?Gly?Thr?Asp?Leu
205 210 215
acg?atc?gag?ctt?ccc?gag?aag?cat?ctg?tgg?gtc?ggc?gcc?gga?agc?aca 1205
Thr?Ile?Glu?Leu?Pro?Glu?Lys?His?Leu?Trp?Val?Gly?Ala?Gly?Ser?Thr
220 225 230 235
aac?gaa?aac?ggc?gtc?gag?ttc?atg?gcc?aat?atg?ccg?aca?gag?gaa?gta 1253
Asn?Glu?Asn?Gly?Val?Glu?Phe?Met?Ala?Asn?Met?Pro?Thr?Glu?Glu?Val
240 245 250
ttc?act?gct?ccg?aaa?aaa?gac?ggg?gtg?aac?ggc?acc?gtt?tca?agc?acg 1301
Phe?Thr?Ala?Pro?Lys?Lys?Asp?Gly?Val?Asn?Gly?Thr?Val?Ser?Ser?Thr
255 260 265
aag?ccg?tta?agc?tat?ggc?ggc?aat?tta?atc?gac?ggc?ttt?aca?ctg?aca 1349
Lys?Pro?Leu?Ser?Tyr?Gly?Gly?Asn?Leu?Ile?Asp?Gly?Phe?Thr?Leu?Thr
270 275 280
ttt?aaa?aac?gga?cgg?att?acg?gat?gtc?aaa?gct?gga?caa?ggc?gaa?gaa 1397
Phe?Lys?Asn?Gly?Arg?Ile?Thr?Asp?Val?Lys?Ala?Gly?Gln?Gly?Glu?Glu
285 290 295
att?tta?aaa?gag?ctg?atc?gaa?acg?gat?gag?ggt?tca?cac?tac?tta?ggc 1445
Ile?Leu?Lys?Glu?Leu?Ile?Glu?Thr?Asp?Glu?Gly?Ser?His?Tyr?Leu?Gly
300 305 310 315
gaa?att?gcg?ctt?gtt?gcc?cac?gat?tcg?ccg?att?tcc?aag?tcc?aat?att 1493
Glu?Ile?Ala?Leu?Val?Ala?His?Asp?Ser?Pro?Ile?Ser?Lys?Ser?Asn?Ile
320 325 330
ctg?ttt?tac?aac?aca?ctg?ttt?gat?gaa?aat?gcc?tca?aac?cac?ctc?gcg 1541
Leu?Phe?Tyr?Asn?Thr?Leu?Phe?Asp?Glu?Asn?Ala?Ser?Asn?His?Leu?Ala
335 340 345
atc?ggc?agt?gca?tac?gca?ttc?aac?atc?gaa?ggc?gga?aaa?cag?atg?tcg 1589
Ile?Gly?Ser?Ala?Tyr?Ala?Phe?Asn?Ile?Glu?Gly?Gly?Lys?Gln?Met?Ser
350 355 360
cgc?gag?gag?ctg?gcg?aaa?gaa?gga?tta?aac?gaa?agc?atc?aca?cat?gtt 1637
Arg?Glu?Glu?Leu?Ala?Lys?Glu?Gly?Leu?Asn?Glu?Ser?Ile?Thr?His?Val
365 370 375
gac?ttt?atg?atc?ggt?tca?gga?gaa?atg?aat?ata?gac?gga?atc?act?gct 1685
Asp?Phe?Met?Ile?Gly?Ser?Gly?Glu?Met?Asn?Ile?Asp?Gly?Ile?Thr?Ala
380 385 390 395
gac?gga?aaa?aga?gag?ccg?ata?ttc?aga?aac?ggc?aac?tgg?gcg?ata 1730
Asp?Gly?Lys?Arg?Glu?Pro?Ile?Phe?Arg?Asn?Gly?Asn?Trp?Ala?Ile
400 405 410
taaagccttc?cgcaaaaaaa?gacctcagaa?aacttctgag?gtccttttat?atcgtgtccg 1790
catgccgccc?gcaatcctaa?acttccctgt?acatgcatgg?cggctgtaac?agctgaaaat 1850
caggtccatg?ctccgccgcc?tcatcgcttg?ggcaaactgc?ttaaatgttc?atgcttcagt 1910
ttccagcgtc?ctgactcgtc?ctttacaaat?acgtttgtcg?cacggcctgc?cccttgaacc 1970
ggttcgcccc?tatataaccc?tttccattcg?tatgtataaa?gacagacggc?ctgttcgtcc 2030
gtttccgtga?tccagctgac?atgaccggcg?ctgtatactt?cattttcgat?ttgcttccag 2090
gcgttttcaa?aataacgctg?aatctcctca?aagcccgagc?acgtttgatc?cgaaaaccag 2150
tacacagcgc?cggggtcaag?gagggctttg?acattctcaa?actcatgtgt?gtttgttgct 2210
tgaatatatt?gctcaagagc 2230
<210>18
<211>410
<212>PRT
＜213〉Bacillus licheniformis
<400>18
Met?Asn?Ser?Phe?Thr?Asp?Lys?Leu?Ser?Lys?Tyr?Ala?Lys?Leu?Val?Ala
1 5 10 15
Glu?Val?Gly?Val?Asn?Val?Gln?Lys?Gly?Gln?Gln?Val?Val?Ile?Asn?Ala
20 25 30
Ser?Thr?Glu?Val?Arg?Asp?Phe?Ala?Arg?Leu?Leu?Val?Lys?Ser?Ala?Tyr
35 40 45
Gln?Arg?Gly?Ala?Lys?Asn?Val?Thr?Val?Arg?Trp?Gln?Asp?Asp?Glu?Val
50 55 60
Thr?Arg?Leu?Lys?Tyr?Glu?Leu?Ala?Pro?Ala?Glu?Ala?Phe?Glu?Glu?Phe
65 70 75 80
Pro?Glu?Trp?Glu?Ala?Lys?Gly?Leu?Glu?Thr?Leu?Ala?Lys?Glu?Gly?Ala
85 90 95
Ala?Phe?Ile?Ser?Ile?Val?Ser?Ser?Ser?Pro?Asp?Leu?Leu?Lys?Gly?Ile
100 105 110
Asp?Ser?Lys?Lys?Ile?Ser?Ala?Gln?Gln?Lys?Ala?Ala?Gly?Lys?Ala?Met
115 120 125
His?Thr?Tyr?Arg?Gln?Met?Ile?Gln?Ser?Asp?Lys?Val?Ser?Trp?Thr?Val
130 135 140
Val?Ala?Ala?Pro?Ser?Pro?Ala?Trp?Ala?Lys?Lys?Val?Phe?Pro?Glu?Ala
145 150 155 160
Asp?Glu?Glu?Thr?Ala?Val?Gln?Lys?Leu?Trp?Asn?Glu?Ile?Phe?Lys?Thr
165 170 175
Thr?Arg?Val?Asp?Arg?Glu?Asp?Pro?Val?Gln?Ala?Trp?Lys?Asp?His?Asp
180 185 190
Gln?Thr?Leu?Arg?Asp?Lys?Val?Ser?Val?Leu?Asn?Asp?Lys?His?Tyr?His
195 200 205
Ala?Leu?His?Tyr?Gln?Ala?Lys?Gly?Thr?Asp?Leu?Thr?Ile?Glu?Leu?Pro
210 215 220
Glu?Lys?His?Leu?Trp?Val?Gly?Ala?Gly?Ser?Thr?Asn?Glu?Asn?Gly?Val
225 230 235 240
Glu?Phe?Met?Ala?Asn?Met?Pro?Thr?Glu?Glu?Val?Phe?Thr?Ala?Pro?Lys
245 250 255
Lys?Asp?Gly?Val?Asn?Gly?Thr?Val?Ser?Ser?Thr?Lys?Pro?Leu?Ser?Tyr
260 265 270
Gly?Gly?Asn?Leu?Ile?Asp?Gly?Phe?Thr?Leu?Thr?Phe?Lys?Asn?Gly?Arg
275 280 285
Ile?Thr?Asp?Val?Lys?Ala?Gly?Gln?Gly?Glu?Glu?Ile?Leu?Lys?Glu?Leu
290 295 300
Ile?Glu?Thr?Asp?Glu?Gly?Ser?His?Tyr?Leu?Gly?Glu?Ile?Ala?Leu?Val
305 310 315 320
Ala?His?Asp?Ser?Pro?Ile?Ser?Lys?Ser?Asn?Ile?Leu?Phe?Tyr?Asn?Thr
325 330 335
Leu?Phe?Asp?Glu?Asn?Ala?Ser?Asn?His?Leu?Ala?Ile?Gly?Ser?Ala?Tyr
340 345 350
Ala?Phe?Asn?Ile?Glu?Gly?Gly?Lys?Gln?Met?Ser?Arg?Glu?Glu?Leu?Ala
355 360 365
Lys?Glu?Gly?Leu?Asn?Glu?Ser?Ile?Thr?His?Val?Asp?Phe?Met?Ile?Gly
370 375 380
Ser?Gly?Glu?Met?Asn?Ile?Asp?Gly?Ile?Thr?Ala?Asp?Gly?Lys?Arg?Glu
385 390 395 400
Pro?Ile?Phe?Arg?Asn?Gly?Asn?Trp?Ala?Ile
405 410
<210>19
<211>2390
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(1273)..(2388)
<400>19
caggtgaaaa?agatataaaa?atgaaagggc?cgatatcaat?cggccttttc?atattaccaa 60
aaaaacaagc?ctgcaagagc?cacaccggca?attagaccga?ggccaagtcc?gtatacaggg 120
ccccaacccc?accagccgcc?gtaatatccg?taggcgaacc?cgcggcctcc?ccgtctgatc 180
atcggggcaa?tatatacttt?gcttggtgaa?acacggacga?ttcttccgac?atggacgcgg 240
cctcctttgt?cggtgattct?cgcaactctg?ccaatatttt?ggtggcaaaa?cctataatat 300
cggtccactt?gaatgctcct?tccatcatct?aatctattcc?tagtcagctt?atgacggatt 360
taatagaaaa?ggttggacga?aagaaggagg?acaaatgatg?atttatagat?gtgatataaa 420
aataatatga?tcgagagagt?cccggtattt?ttccaggtaa?tgcaggtgtt?tttccatcgt 480
ttcatagtct?ttgtcggaaa?ttgcaggcga?cagatccatt?tcaacggcgt?catccgggtc 540
tcctgcatct?tccaatacga?caggaatgat?gtcaatcttt?gaaaatcctt?cttctcgaag 600
gacattgacc?cactcatcct?cgtcatataa?tgcggtgaat?ccgtaaaatt?ccgtgatttc 660
tttttttgct?tcgtctgtca?gtgcgtttcc?ttccttaagg?cagacttcga?tcgcgaccag 720
catccctccg?ggttttaaaa?tccgccggat?ttcctgtaaa?gcagaccgga?tatttgtgaa 780
gctcagcaca?gattcagtta?atacacaggc?aaagctttca?gactgaaacg?gcatttcctc 840
aagtggagag?cggacagccg?gaatggaaag?ctgttctttt?ttaaaacgct?gtcgggcttt 900
atcgatcatg?accgggtcgg?catcaagggc?agtgacagga?aacagaagct?gacccaaata 960
agcggcggtc?tggccggttc?cgcagcctgc?atcaagaatc?ggctgctcag?ggtcgatgcc 1020
ggctttggcg?agcactgcct?ttgacaggga?catcccgccg?ggatgagcgg?atgagacgcc 1080
gaagctggcg?agcatgtcta?aataatcgct?caaattgaga?tccctccttt?tttgtatgca 1140
tatgcaaaag?cggaataatg?gttgaaaacg?atcgaatgcc?agcatcccgg?aaaaatcggt 1200
atttaagtga?ttcgagttga?cgaataaaga?ttcgtccatg?ataatatggt?aagaagaaaa 1260
ggtggtgttc?gt?ttg?aaa?cag?cag?ttg?aaa?ctc?ttt?ttg?gct?gta?ttg?att 1311
Leu?Lys?Gln?Gln?Leu?Lys?Leu?Phe?Leu?Ala?Val?Leu?Ile
l 5 10
acg?gcc?gtc?gcc?gcc?tcc?gca?ctg?acg?ctt?tat?ctg?gtg?ggc?aaa?ggc 1359
Thr?Ala?Val?Ala?Ala?Ser?Ala?Leu?Thr?Leu?Tyr?Leu?Val?Gly?Lys?Gly
15 20 25
agc?ggc?ggt?tca?gta?tat?tcc?gct?tca?gat?tcc?gag?aaa?ttc?gaa?aag 1407
Ser?Gly?Gly?Ser?Val?Tyr?Ser?Ala?Ser?Asp?Ser?Glu?Lys?Phe?Glu?Lys
30 35 40 45
ctg?atg?gcg?gcg?tac?gac?aaa?atc?aag?agc?gac?tat?tat?caa?aat?gca 1455
Leu?Met?Ala?Ala?Tyr?Asp?Lys?Ile?Lys?Ser?Asp?Tyr?Tyr?Gln?Asn?Ala
50 55 60
gac?gat?gaa?aag?ctg?act?gac?ggc?gcg?att?aaa?ggg?atg?ctt?gga?gct 1503
Asp?Asp?Glu?Lys?Leu?Thr?Asp?Gly?Ala?Ile?Lys?Gly?Met?Leu?Gly?Ala
65 70 75
ttg?gat?gat?cca?tat?tct?aca?tac?atg?gat?aag?aaa?gaa?gct?aaa?agc 1551
Leu?Asp?Asp?Pro?Tyr?Ser?Thr?Tyr?Met?Asp?Lys?Lys?Glu?Ala?Lys?Ser
80 85 90
ttt?gaa?gaa?agc?att?aca?tct?tct?ttt?gaa?gga?atc?ggc?gcc?caa?gta 1599
Phe?Glu?Glu?Ser?Ile?Thr?Ser?Ser?Phe?Glu?Gly?Ile?Gly?Ala?Gln?Val
95 100 105
gaa?gaa?aaa?aat?gga?caa?att?cta?atc?gtc?gcg?cct?att?aaa?gga?tct 1647
Glu?Glu?Lys?Asn?Gly?Gln?Ile?Leu?Ile?Val?Ala?Pro?Ile?Lys?Gly?Ser
110 115 120 125
ccc?gca?gaa?aaa?gcc?ggc?tta?aag?ccg?cat?gat?gaa?att?cag?aaa?gtg 1695
Pro?Ala?Glu?Lys?Ala?Gly?Leu?Lys?Pro?His?Asp?Glu?Ile?Gln?Lys?Val
130 135 140
gac?ggc?aaa?agc?gta?aaa?ggc?aag?acc?gtc?aac?gaa?gcg?aca?gcg?atg 1743
Asp?Gly?Lys?Ser?Val?Lys?Gly?Lys?Thr?Val?Asn?Glu?Ala?Thr?Ala?Met
145 150 155
atc?cgc?ggc?aaa?aaa?ggc?acg?aat?gtc?aaa?ctt?gtt?ctc?aac?agg?gaa 1791
Ile?Arg?Gly?Lys?Lys?Gly?Thr?Asn?Val?Lys?Leu?Val?Leu?Asn?Arg?Glu
160 165 170
ggc?gtc?ggc?caa?atc?gat?gtt?acg?att?aag?cgc?gat?acg?att?ccg?atc 1839
Gly?Val?Gly?Gln?Ile?Asp?Val?Thr?Ile?Lys?Arg?Asp?Thr?Ile?Pro?Ile
175 180 185
gaa?acg?gtt?tat?tcc?aaa?atg?atc?gac?ggc?aat?atc?ggt?gaa?atc?caa 1887
Glu?Thr?Val?Tyr?Ser?Lys?Met?Ile?Asp?Gly?Asn?Ile?Gly?Glu?Ile?Gln
190 195 200 205
att?acg?tct?ttc?tct?gaa?aat?acc?gca?aaa?gaa?ctg?aca?aaa?gcc?att 1935
Ile?Thr?Ser?Phe?Ser?Glu?Asn?Thr?Ala?Lys?Glu?Leu?Thr?Lys?Ala?Ile
210 215 220
gat?gac?ttg?tct?gaa?aaa?ggt?gcg?gaa?cga?ttc?gtg?ctg?gat?ctc?aga 1983
Asp?Asp?Leu?Ser?Glu?Lys?Gly?Ala?Glu?Arg?Phe?Val?Leu?Asp?Leu?Arg
225 230 235
gga?aat?cca?ggc?ggg?ctg?atg?gac?caa?gcg?att?atg?atg?agc?aac?atg 2031
Gly?Asn?Pro?Gly?Gly?Leu?Met?Asp?Gln?Ala?Ile?Met?Met?Ser?Asn?Met
240 245 250
ttt?gtc?gat?aaa?ggc?aaa?acc?atc?atg?cag?gtt?gaa?tcc?aaa?aac?gga 2079
Phe?Val?Asp?Lys?Gly?Lys?Thr?Ile?Met?Gln?Val?Glu?Ser?Lys?Asn?Gly
255 260 265
aaa?aaa?gaa?gtg?tac?aaa?gct?gag?aag?gag?cga?aaa?gtg?aac?aag?ccg 2127
Lys?Lys?Glu?Val?Tyr?Lys?Ala?Glu?Lys?Glu?Arg?Lys?Val?Asn?Lys?Pro
270 275 280 285
acc?gtt?gtt?ctc?gtc?aac?gga?gga?acg?gca?agc?gcc?gct?gag?atc?atg 2175
Thr?Val?Val?Leu?Val?Asn?Gly?Gly?Thr?Ala?Ser?Ala?Ala?Glu?Ile?Met
290 295 300
gcc?gca?gca?ctt?cac?cag?tct?tca?ggc?atc?ccg?atc?gtc?ggt?gaa?aat 2223
Ala?Ala?Ala?Leu?His?Gln?Ser?Ser?Gly?Ile?Pro?Ile?Val?Gly?Glu?Asn
305 310 315
acg?ttt?ggg?aaa?ggg?acg?gtt?caa?aat?gcg?gag?agc?ttc?tcc?gac?gga 2271
Thr?Phe?Gly?Lys?Gly?Thr?Val?Gln?Asn?Ala?Glu?Ser?Phe?Ser?Asp?Gly
320 325 330
tcg?acc?gtc?aag?ctg?acg?att?gcc?aag?tgg?ctg?acg?ccg?aat?ggg?gac 2319
Ser?Thr?Val?Lys?Leu?Thr?Ile?Ala?Lys?Trp?Leu?Thr?Pro?Asn?Gly?Asp
335 340 345
tgg?att?cac?gaa?aaa?gga?atc?aaa?cct?caa?tat?aaa?gcg?gag?ctc?cca 2367
Trp?Ile?His?Glu?Lys?Gly?Ile?Lys?Pro?Gln?Tyr?Lys?Ala?Glu?Leu?Pro
350 355 360 365
agc?tat?gcc?aaa?ctg?ccg?tac?ct 2390
Ser?Tyr?Ala?Lys?Leu?Pro?Tyr
370
<210>20
<211>372
<212>PRT
＜213〉Bacillus licheniformis
<400>20
Leu?Lys?Gln?Gln?Leu?Lys?Leu?Phe?Leu?Ala?Val?Leu?Ile?Thr?Ala?Val
1 5 10 15
Ala?Ala?Ser?Ala?Leu?Thr?Leu?Tyr?Leu?Val?Gly?Lys?Gly?Ser?Gly?Gly
20 25 30
Ser?Val?Tyr?Ser?Ala?Ser?Asp?Ser?Glu?Lys?Phe?Glu?Lys?Leu?Met?Ala
35 40 45
Ala?Tyr?Asp?Lys?Ile?Lys?Ser?Asp?Tyr?Tyr?Gln?Asn?Ala?Asp?Asp?Glu
50 55 60
Lys?Leu?Thr?Asp?Gly?Ala?Ile?Lys?Gly?Met?Leu?Gly?Ala?Leu?Asp?Asp
65 70 75 80
Pro?Tyr?Ser?Thr?Tyr?Met?Asp?Lys?Lys?Glu?Ala?Lys?Ser?Phe?Glu?Glu
85 90 95
Ser?Ile?Thr?Ser?Ser?Phe?Glu?Gly?Ile?Gly?Ala?Gln?Val?Glu?Glu?Lys
100 105 110
Asn?Gly?Gln?Ile?Leu?Ile?Val?Ala?Pro?Ile?Lys?Gly?Ser?Pro?Ala?Glu
115 120 125
Lys?Ala?Gly?Leu?Lys?Pro?His?Asp?Glu?Ile?Gln?Lys?Val?Asp?Gly?Lys
130 135 140
Ser?Val?Lys?Gly?Lys?Thr?Val?Asn?Glu?Ala?Thr?Ala?Met?Ile?Arg?Gly
145 150 155 160
Lys?Lys?Gly?Thr?Asn?Val?Lys?Leu?Val?Leu?Asn?Arg?Glu?Gly?Val?Gly
165 170 175
Gln?Ile?Asp?Val?Thr?Ile?Lys?Arg?Asp?Thr?Ile?Pro?Ile?Glu?Thr?Val
180 185 190
Tyr?Ser?Lys?Met?Ile?Asp?Gly?Asn?Ile?Gly?Glu?Ile?Gln?Ile?Thr?Ser
195 200 205
Phe?Ser?Glu?Asn?Thr?Ala?Lys?Glu?Leu?Thr?Lys?Ala?Ile?Asp?Asp?Leu
210 215 220
Ser?Glu?Lys?Gly?Ala?Glu?Arg?Phe?Val?Leu?Asp?Leu?Arg?Gly?Asn?Pro
225 230 235 240
Gly?Gly?Leu?Met?Asp?Gln?Ala?Ile?Met?Met?Ser?Asn?Met?Phe?Val?Asp
245 250 255
Lys?Gly?Lys?Thr?Ile?Met?Gln?Val?Glu?Ser?Lys?Asn?Gly?Lys?Lys?Glu
260 265 270
Val?Tyr?Lys?Ala?Glu?Lys?Glu?Arg?Lys?Val?Asn?Lys?Pro?Thr?Val?Val
275 280 285
Leu?Val?Asn?Gly?Gly?Thr?Ala?Ser?Ala?Ala?Glu?Ile?Met?Ala?Ala?Ala
290 295 300
Leu?His?Gln?Ser?Ser?Gly?Ile?Pro?Ile?Val?Gly?Glu?Asn?Thr?Phe?Gly
305 310 315 320
Lys?Gly?Thr?Val?Gln?Asn?Ala?Glu?Ser?Phe?Ser?Asp?Gly?Ser?Thr?Val
325 330 335
Lys?Leu?Thr?Ile?Ala?Lys?Trp?Leu?Thr?Pro?Asn?Gly?Asp?Trp?Ile?His
340 345 350
Glu?Lys?Gly?Ile?Lys?Pro?Gln?Tyr?Lys?Ala?Glu?Leu?Pro?Ser?Tyr?Ala
355 360 365
Lys?Leu?Pro?Tyr
370
<210>21
<211>2772
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1406)
<400>21
ttcttgaatc?tgcggatata?ttggcgtaag?tattgtacga?taaatccaaa?tagcgaccca 60
ccctaaacac?aatgcaataa?ttatcttttt?gtaataattg?gggtcatgtt?taattgtttt 120
ttccacggta?tgagaacgct?cccttctttt?aatctatttt?ttatttaatt?aaattgtagg 180
ttgtaagcca?gtcaataaca?ttgttcactt?tcaacaaaaa?caaaagtaac?attatacatt 240
ttagacaaaa?ggcgtcgctt?aaaaatgatt?catcgataga?agctggcctt?atgactcttt 300
tttaaaacta?acttggagta?cccaatttaa?cttcccgaat?aaacaggaag?cttcacaaat 360
gttgatataa?cagtgttttt?aacgttttaa?aaactaaagg?tgcatgacaa?gcgaccttaa 420
gataagcttt?ctttctcttt?gaaaattcaa?aaatcctccg?atatatatag?cgaacgccgc 480
taaaaaagga?ggagagccgg?atg?aaa?aag?tta?tgg?aaa?atc?gcg?gtt?tcg?gct 533
Met?Lys?Lys?Leu?Trp?Lys?Ile?Ala?Val?Ser?Ala
1 5 10
gca?atg?ttc?gtc?ggt?ttt?ttc?gca?aat?tca?ccc?cgc?att?caa?gcg?gaa 581
Ala?Met?Phe?Val?Gly?Phe?Phe?Ala?Asn?Ser?Pro?Arg?Ile?Gln?Ala?Glu
15 20 25
agc?aat?aag?cag?gaa?aac?gag?gtc?att?gtt?gta?tat?aaa?aat?acc?agc 629
Ser?Asn?Lys?Gln?Glu?Asn?Glu?Val?Ile?Val?Val?Tyr?Lys?Asn?Thr?Ser
30 35 40
gga?aaa?gaa?acc?gtc?atc?gaa?cag?gca?gac?acg?gta?gaa?cac?gtt?tac 677
Gly?Lys?Glu?Thr?Val?Ile?Glu?Gln?Ala?Asp?Thr?Val?Glu?His?Val?Tyr
45 50 55
cgg?cac?att?ccc?gca?gcc?gct?gtc?act?gcg?gac?gac?aaa?aca?gtg?cgc 725
Arg?His?Ile?Pro?Ala?Ala?Ala?Val?Thr?Ala?Asp?Asp?Lys?Thr?Val?Arg
60 65 70 75
gaa?ctt?gaa?cac?gat?ccc?gac?gtc?ctg?tat?gtc?gaa?gac?aac?ctc?ccg 773
Glu?Leu?Glu?His?Asp?Pro?Asp?Val?Leu?Tyr?Val?Glu?Asp?Asn?Leu?Pro
80 85 90
gta?gct?gct?gcc?gac?agc?acc?gct?cta?aaa?gct?ttc?tcc?agc?agc?aca 821
Val?Ala?Ala?Ala?Asp?Ser?Thr?Ala?Leu?Lys?Ala?Phe?Ser?Ser?Ser?Thr
95 100 105
gcg?caa?aac?gcc?tcc?gcg?ttt?tca?cag?tgg?aac?atc?aag?ctg?att?caa 869
Ala?Gln?Asn?Ala?Ser?Ala?Phe?Ser?Gln?Trp?Asn?Ile?Lys?Leu?Ile?Gln
110 115 120
gcc?gca?ctg?gct?tgg?aat?aaa?ggt?ttg?acc?gga?aaa?cag?gtg?aag?atc 917
Ala?Ala?Leu?Ala?Trp?Asn?Lys?Gly?Leu?Thr?Gly?Lys?Gln?Val?Lys?Ile
125 130 135
gcg?gtt?att?gac?agc?ggg?att?tcc?ccc?cat?gag?gag?ctg?tcg?atc?gcc 965
Ala?Val?Ile?Asp?Ser?Gly?Ile?Ser?Pro?His?Glu?Glu?Leu?Ser?Ile?Ala
140 145 150 155
ggc?ggt?gca?tcg?atg?gtc?ggc?tat?acc?gct?tca?tac?cgt?gac?gat?aat 1013
Gly?Gly?Ala?Ser?Met?Val?Gly?Tyr?Thr?Ala?Ser?Tyr?Arg?Asp?Asp?Asn
160 165 170
ggc?cac?gga?acc?cat?gtt?gcc?gga?atc?atc?gga?gcg?aag?cat?aac?gga 1061
Gly?His?Gly?Thr?His?Val?Ala?Gly?Ile?Ile?Gly?Ala?Lys?His?Asn?Gly
175 180 185
cgg?ggg?atc?gac?ggc?atc?gcg?ccc?ggc?gcg?cag?ctg?tat?gcc?gta?aaa 1109
Arg?Gly?Ile?Asp?Gly?Ile?Ala?Pro?Gly?Ala?Gln?Leu?Tyr?Ala?Val?Lys
190 195 200
gcg?ctg?gac?cgg?aat?ggt?gcg?ggg?gat?ctg?aaa?ggt?atc?tta?caa?ggc 1157
Ala?Leu?Asp?Arg?Asn?Gly?Ala?Gly?Asp?Leu?Lys?Gly?Ile?Leu?Gln?Gly
205 210 215
atc?gat?tgg?tcg?atc?caa?cac?gga?atc?gat?atc?atc?aat?atg?agc?ctc 1205
Ile?Asp?Trp?Ser?Ile?Gln?His?Gly?Ile?Asp?Ile?Ile?Asn?Met?Ser?Leu
220 225 230 235
gtc?gtt?tca?ggc?gac?agt?caa?gtt?ctc?cac?gat?gcg?gta?gat?aaa?gca 1253
Val?Val?Ser?Gly?Asp?Ser?Gln?Val?Leu?His?Asp?Ala?Val?Asp?Lys?Ala
240 245 250
tac?aaa?cgg?gga?atc?att?tta?gtc?gga?gcg?agc?gga?aat?gcc?gga?aac 1301
Tyr?Lys?Arg?Gly?Ile?Ile?Leu?Val?Gly?Ala?Ser?Gly?Asn?Ala?Gly?Asn
255 260 265
gga?aaa?tcc?gtt?tat?tac?ccg?gcc?gcc?tac?agc?agc?gtc?att?gcc?gtc 1349
Gly?Lys?Ser?Val?Tyr?Tyr?Pro?Ala?Ala?Tyr?Ser?Ser?Val?Ile?Ala?Val
270 275 280
tcg?gca?acc?aat?gaa?aaa?aat?cag?atc?gcc?tca?ttt?acc?aac?acc?cgg 1397
Ser?Ala?Thr?Asn?Glu?Lys?Asn?Gln?Ile?Ala?Ser?Phe?Thr?Asn?Thr?Arg
285 290 295
agc?gcc?gta?tagtattccg?ctccgagcac?atctattatc?aatacatcgg 1446
Ser?Ala?Val
300
gcaatcgcgg?atatgcaatc?ggaagctgca?ctgcacaagg?gacacccgac?gtcacctgcg 1506
tgatcgcact?tatgaaacag?ctgcacccaa?ccgcttcaaa?tgctgagctg?cgcaagaaaa 1566
tgcagtttta?tacaagcgat?ttgggcgctc?ccggtcgtga?tcatttgttc?ggctaccgat 1626
taatccgctt?caaagaggtc?acacagccat?tggaaaaagc?gcaaaaagcc?gtcggacaag 1686
cggaaaagac?aaagaaaaaa?gcggacattc?aaaccgcaca?aaaagcgatc?gaaccgctcc 1746
ctgcagacgc?agataaaccc?gctttgaaaa?agagactcaa?tactgttaaa?gagcagctta 1806
aaaaaacagc?cgaaagcaaa?gtgaaactcg?ctgagaagca?aaaaaagaaa?accaatgccg 1866
actccgccca?aaaagcggtc?aacgaactcg?acagcggcac?tttcaaaacg?aatttgcaaa 1926
aacggatcaa?cgcggtccgt?tcaagcctct?tgaagaccgc?aaagcaggcc?gttgccaaag 1986
ctgaaaaagc?ggcaacagac?tcaaacctcg?gcaaagcgca?aaaagcgatc?aatgagcttc 2046
ccgccggaaa?agacaaatca?aatctgcaaa?aaaggctgaa?caccgcgaaa?aagcaagcag 2106
ctgcagccta?taataaaaaa?gtttctgcag?caaaggccaa?agtcaaaacg?gccgaacaaa 2166
agagaacgaa?aaaaacaaaa?tcagcagcgc?aatcagcagt?aggcaagctg?aaagcatccg 2226
ccgaaaaaac?gaagctgcaa?aaacggatca?atgccattaa?gctgaagtag?tacggaaaaa 2286
agcccggaat?catccgggcc?ttttaatttt?catatttagc?aaatgcgtca?ttattgatgc 2346
tgttgtacta?attgacattt?cgggctcgtc?atttgcgtaa?tcgtattcct?tggtcggccg 2406
cttactctcc?cccgtactct?ccaatcactt?cttatcaatt?ctttccgtca?caatcccgac 2466
gatgtcccgt?tctgaaacaa?agccgaaatg?gcggctgtca?aagctgtgga?tccggttgtc 2526
cccgaggacg?aagtactcgt?tttcaggcac?cttctcctct?ccggtcactt?cctgcagtgt 2586
aaaatcccct?gtcaagtccc?ctccggcggt?gacggccttt?aagtcgtcca?gataaggctc 2646
tttgatcttt?tcttcgttga?tatacagctg?atcatcttca?tacctgaggg?tttcgcccgg 2706
aagcccgatc?acccttttaa?tgaatatatc?tttgtcaggt?cctttaaaca?gcacgatgtc 2766
aaatcg 2772
<210>22
<211>302
<212>PRT
＜213〉Bacillus licheniformis
<400>22
Met?Lys?Lys?Leu?Trp?Lys?Ile?Ala?Val?Ser?Ala?Ala?Met?Phe?Val?Gly
1 5 10 15
Phe?Phe?Ala?Asn?Ser?Pro?Arg?Ile?Gln?Ala?Glu?Ser?Asn?Lys?Gln?Glu
20 25 30
Asn?Glu?Val?Ile?Val?Val?Tyr?Lys?Asn?Thr?Ser?Gly?Lys?Glu?Thr?Val
35 40 45
Ile?Glu?Gln?Ala?Asp?Thr?Val?Glu?His?Val?Tyr?Arg?His?Ile?Pro?Ala
50 55 60
Ala?Ala?Val?Thr?Ala?Asp?Asp?Lys?Thr?Val?Arg?Glu?Leu?Glu?His?Asp
65 70 75 80
Pro?Asp?Val?Leu?Tyr?Val?Glu?Asp?Asn?Leu?Pro?Val?Ala?Ala?Ala?Asp
85 90 95
Ser?Thr?Ala?Leu?Lys?Ala?Phe?Ser?Ser?Ser?Thr?Ala?Gln?Asn?Ala?Ser
100 105 110
Ala?Phe?Ser?Gln?Trp?Asn?Ile?Lys?Leu?Ile?Gln?Ala?Ala?Leu?Ala?Trp
115 120 125
Asn?Lys?Gly?Leu?Thr?Gly?Lys?Gln?Val?Lys?Ile?Ala?Val?Ile?Asp?Ser
130 135 140
Gly?Ile?Ser?Pro?His?Glu?Glu?Leu?Ser?Ile?Ala?Gly?Gly?Ala?Ser?Met
145 150 155 160
Val?Gly?Tyr?Thr?Ala?Ser?Tyr?Arg?Asp?Asp?Asn?Gly?His?Gly?Thr?His
165 170 175
Val?Ala?Gly?Ile?Ile?Gly?Ala?Lys?His?Asn?Gly?Arg?Gly?Ile?Asp?Gly
180 185 190
Ile?Ala?Pro?Gly?Ala?Gln?Leu?Tyr?Ala?Val?Lys?Ala?Leu?Asp?Arg?Asn
195 200 205
Gly?Ala?Gly?Asp?Leu?Lys?Gly?Ile?Leu?Gln?Gly?Ile?Asp?Trp?Ser?Ile
210 215 220
Gln?His?Gly?Ile?Asp?Ile?Ile?Asn?Met?Ser?Leu?Val?Val?Ser?Gly?Asp
225 230 235 240
Ser?Gln?Val?Leu?His?Asp?Ala?Val?Asp?Lys?Ala?Tyr?Lys?Arg?Gly?Ile
245 250 255
Ile?Leu?Val?Gly?Ala?Ser?Gly?Asn?Ala?Gly?Asn?Gly?Lys?Ser?Val?Tyr
260 265 270
Tyr?Pro?Ala?Ala?Tyr?Ser?Ser?Val?Ile?Ala?Val?Ser?Ala?Thr?Asn?Glu
275 280 285
Lys?Asn?Gln?Ile?Ala?Ser?Phe?Thr?Asn?Thr?Arg?Ser?Ala?Val
290 295 300
<210>23
<211>2185
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(214)..(1149)
<400>23
atcagggcat?gcagacggac?cggtttattt?tcaacctcgg?acacgggatc?tttccagaag 60
tgagtcccga?cacactgaaa?aaattgacgg?catttgttca?tgagtattcc?gcctcaaaaa 120
aatcgagcct?gatcacatga?gcatttcagt?tggtcttttc?aatataaact?gtcaaaatac 180
gtatagatca?tgatctttaa?agaggtgttc?acg?ttg?ggt?aaa?aag?aaa?atg?ggg 234
Leu?Gly?Lys?Lys?Lys?Met?Gly
ctt?ctt?gtc?atg?gca?tat?ggg?acg?ccg?tac?aaa?gag?gaa?gac?att?gaa 282
Leu?Leu?Val?Met?Ala?Tyr?Gly?Thr?Pro?Tyr?Lys?Glu?Glu?Asp?Ile?Glu
10 15 20
cgt?tat?tat?aca?cat?atc?aga?aga?ggc?aga?aag?cct?gag?cct?gac?atg 330
Arg?Tyr?Tyr?Thr?His?Ile?Arg?Arg?Gly?Arg?Lys?Pro?Glu?Pro?Asp?Met
25 30 35
ctt?cag?gac?tta?aaa?gac?cgc?tat?aag?gcg?atc?gga?gga?att?tct?ccg 378
Leu?Gln?Asp?Leu?Lys?Asp?Arg?Tyr?Lys?Ala?Ile?Gly?Gly?Ile?Ser?Pro
40 45 50 55
ctt?gca?aag?atc?acg?cag?gag?cag?acg?aag?cag?ctg?gaa?aaa?cgc?tta 426
Leu?Ala?Lys?Ile?Thr?Gln?Glu?Gln?Thr?Lys?Gln?Leu?Glu?Lys?Arg?Leu
60 65 70
aac?gag?ctt?cag?gat?gat?gtg?gtg?ttc?aag?gca?tat?atc?ggt?ctc?aaa 474
Asn?Glu?Leu?Gln?Asp?Asp?Val?Val?Phe?Lys?Ala?Tyr?Ile?Gly?Leu?Lys
75 80 85
cat?atc?gaa?cct?ttc?att?gag?gat?gcc?gtt?cgg?gag?atg?cat?aaa?gac 522
His?Ile?Glu?Pro?Phe?Ile?Glu?Asp?Ala?Val?Arg?Glu?Met?His?Lys?Asp
90 95 100
ggg?att?aca?gaa?gcc?gtc?agc?atc?gtg?ctc?gcg?ccc?cat?ttc?tcc?acc 570
Gly?Ile?Thr?Glu?Ala?Val?Ser?Ile?Val?Leu?Ala?Pro?His?Phe?Ser?Thr
105 110 115
ttt?agc?gta?cag?tcg?tac?aac?aag?cgg?gcg?aaa?gac?gag?gct?gaa?aag 618
Phe?Ser?Val?Gln?Ser?Tyr?Asn?Lys?Arg?Ala?Lys?Asp?Glu?Ala?Glu?Lys
120 125 130 135
ctc?ggc?gaa?ttg?tcg?atc?aca?tcg?atc?aac?agc?tgg?tat?gat?gag?ccg 666
Leu?Gly?Glu?Leu?Ser?Ile?Thr?Ser?Ile?Asn?Ser?Trp?Tyr?Asp?Glu?Pro
140 145 150
aaa?ttt?atc?gct?tat?tgg?gcg?gat?cag?gtg?aga?aaa?atc?tat?gac?gaa 714
Lys?Phe?Ile?Ala?Tyr?Trp?Ala?Asp?Gln?Val?Arg?Lys?Ile?Tyr?Asp?Glu
155 160 165
atg?cct?tcg?gaa?gag?agg?gaa?aac?gcc?gtc?ttg?att?gta?tcg?gcg?cac 762
Met?Pro?Ser?Glu?Glu?Arg?Glu?Asn?Ala?Val?Leu?Ile?Val?Ser?Ala?His
170 175 180
agc?ctt?ccc?gaa?aaa?atc?gtc?gaa?atg?ggt?gat?ccg?tat?cct?gaa?cag 810
Ser?Leu?Pro?Glu?Lys?Ile?Val?Glu?Met?Gly?Asp?Pro?Tyr?Pro?Glu?Gln
185 190 195
ctt?aaa?gaa?tca?gcg?aag?ctg?att?gcc?gaa?gcg?gcg?gga?gta?aaa?gat 858
Leu?Lys?Glu?Ser?Ala?Lys?Leu?Ile?Ala?Glu?Ala?Ala?Gly?Val?Lys?Asp
200 205 210 215
tac?gcc?gtc?ggc?tgg?caa?agc?gaa?gga?aac?acc?cct?gat?ccg?tgg?ctg 906
Tyr?Ala?Val?Gly?Trp?Gln?Ser?Glu?Gly?Asn?Thr?Pro?Asp?Pro?Trp?Leu
220 225 230
gga?ccg?gat?gtt?cag?gat?ttg?acg?cgc?gac?ttg?tca?gag?caa?aaa?ggg 954
Gly?Pro?Asp?Val?Gln?Asp?Leu?Thr?Arg?Asp?Leu?Ser?Glu?Gln?Lys?Gly
235 240 245
tac?agc?gct?ttt?gtt?tat?gct?ccc?gtc?ggc?ttt?gta?gcc?gac?cat?ttg 1002
Tyr?Ser?Ala?Phe?Val?Tyr?Ala?Pro?Val?Gly?Phe?Val?Ala?Asp?His?Leu
250 255 260
gaa?gtc?ctt?tat?gac?aat?gac?tat?gag?tgc?aaa?gtc?gtc?acg?gac?gat 1050
Glu?Val?Leu?Tyr?Asp?Asn?Asp?Tyr?Glu?Cys?Lys?Val?Val?Thr?Asp?Asp
265 270 275
atc?gga?gca?agc?tat?tac?aga?cct?gaa?atg?cct?aac?gct?aaa?cat?gag 1098
Ile?Gly?Ala?Ser?Tyr?Tyr?Arg?Pro?Glu?Met?Pro?Asn?Ala?Lys?His?Glu
280 285 290 295
ttt?atc?gat?gct?tta?gcg?gat?gtt?gta?tta?aaa?caa?ttg?gag?aaa?gaa 1146
Phe?Ile?Asp?Ala?Leu?Ala?Asp?Val?Val?Leu?Lys?Gln?Leu?Glu?Lys?Glu
300 305 310
cag?taaagaaggc?gattgtaaat?gagtggagaa?cgcaggcaga?ttgtcatcat 1199
Gln
cggcggcggt?attaccgggt?tggccgccgc?cttctacttg?gagaaggaaa?tcaaaaagaa 1259
tgatctcccg?gccgaagtca?cgcttgttga?agcaagcccg?agactgggcg?gaaagatgca 1319
gacggtacac?aaagacggct?atgtgattga?acggggtccg?gattcatttt?tggaacggaa 1379
acaaagcgca?cctcagctcg?tgaaggatct?cggactggag?catttgcttg?tgaacaatgc 1439
gactggccag?tcttatgtgc?ttgtcagcga?aacgctctat?ccgattccgg?aaggagccgt 1499
gatgggggtg?ccgacaaaaa?tcggtccttt?tctgacaacc?ggcttgtttt?ccttgccggg 1559
gaagttgaga?gccggaatgg?atttggtgct?tccagcaagc?aagccgcagg?aagaccaatc 1619
gctcggagaa?tttttcagaa?ggcgcgtcgg?cggtgaagtg?gtggagaacc?tgatcgaacc 1679
gcttttgtca?ggcatttatg?ccggggacat?cgaccgtctc?agtctgatgt?cgactttccc 1739
gcagttctat?cagacagagc?agaagtacag?aagcttgatc?atcgggatga?aaaaatcgcg 1799
cggcgcccag?agcgtcggaa?agcctgcaaa?caaaaagaaa?ggccagttcc?agacggtgaa 1859
aaccggcctg?cagacgatag?ttgaagaatt?ggaaaagcag?ctcggcatca?caaaggttta 1919
taaaggcaca?aaggtcgtca?gcattgagcg?tgcgggctcg?gcttacagca?tgaagcttga 1979
caacggcaag?gtgcttcacg?ccgattcggc?cgtggtcacc?gctcctcata?aagcagctgc 2039
cgcgatgttt?caaaaagagg?attggctgaa?gggtttagag?gatatggtgt?caacatctgt 2099
cgccaatgtg?gcgctcggat?ttccggaaga?agccgtccaa?atggagcatc?aaggcacggg 2159
ctttgtcatt?tcaagaaaca?gcgatt 2185
<210>24
<211>312
<212>PRT
＜213〉Bacillus licheniformis
<400>24
Leu?Gly?Lys?Lys?Lys?Met?Gly?Leu?Leu?Val?Met?Ala?Tyr?Gly?Thr?Pro
1 5 10 15
Tyr?Lys?Glu?Glu?Asp?Ile?Glu?Arg?Tyr?Tyr?Thr?His?Ile?Arg?Arg?Gly
20 25 30
Arg?Lys?Pro?Glu?Pro?Asp?Met?Leu?Gln?Asp?Leu?Lys?Asp?Arg?Tyr?Lys
35 40 45
Ala?Ile?Gly?Gly?Ile?Ser?Pro?Leu?Ala?Lys?Ile?Thr?Gln?Glu?Gln?Thr
50 55 60
Lys?Gln?Leu?Glu?Lys?Arg?Leu?Asn?Glu?Leu?Gln?Asp?Asp?Val?Val?Phe
65 70 75 80
Lys?Ala?Tyr?Ile?Gly?Leu?Lys?His?Ile?Glu?Pro?Phe?Ile?Glu?Asp?Ala
85 90 95
Val?Arg?Glu?Met?His?Lys?Asp?Gly?Ile?Thr?Glu?Ala?Val?Ser?Ile?Val
100 105 110
Leu?Ala?Pro?His?Phe?Ser?Thr?Phe?Ser?Val?Gln?Ser?Tyr?Asn?Lys?Arg
115 120 125
Ala?Lys?Asp?Glu?Ala?Glu?Lys?Leu?Gly?Glu?Leu?Ser?Ile?Thr?Ser?Ile
130 135 140
Asn?Ser?Trp?Tyr?Asp?Glu?Pro?Lys?Phe?Ile?Ala?Tyr?Trp?Ala?Asp?Gln
145 150 155 160
Val?Arg?Lys?Ile?Tyr?Asp?Glu?Met?Pro?Ser?Glu?Glu?Arg?Glu?Asn?Ala
165 170 175
Val?Leu?Ile?Val?Ser?Ala?His?Ser?Leu?Pro?Glu?Lys?Ile?Val?Glu?Met
180 185 190
Gly?Asp?Pro?Tyr?Pro?Glu?Gln?Leu?Lys?Glu?Ser?Ala?Lys?Leu?Ile?Ala
195 200 205
Glu?Ala?Ala?Gly?Val?Lys?Asp?Tyr?Ala?Val?Gly?Trp?Gln?Ser?Glu?Gly
210 215 220
Asn?Thr?Pro?Asp?Pro?Trp?Leu?Gly?Pro?Asp?Val?Gln?Asp?Leu?Thr?Arg
225 230 235 240
Asp?Leu?Ser?Glu?Gln?Lys?Gly?Tyr?Ser?Ala?Phe?Val?Tyr?Ala?Pro?Val
245 250 255
Gly?Phe?Val?Ala?Asp?His?Leu?Glu?Val?Leu?Tyr?Asp?Asn?Asp?Tyr?Glu
260 265 270
Cys?Lys?Val?Val?Thr?Asp?Asp?Ile?Gly?Ala?Ser?Tyr?Tyr?Arg?Pro?Glu
275 280 285
Met?Pro?Asn?Ala?Lys?His?Glu?Phe?Ile?Asp?Ala?Leu?Ala?Asp?Val?Val
290 295 300
Leu?Lys?Gln?Leu?Glu?Lys?Glu?Gln
305 310
<210>25
<211>2107
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1604)
<400>25
aaaaaagctt?ggcatagcca?agctttctat?tatgcagaaa?gtccgttcac?ttttttcgct 60
aggtttgatt?tgtaacgagc?ggcggtgttt?ttgtggacaa?gacctttttt?agctgcttta 120
tcgattttct?ttgctgctgc?agatagagct?gctttcgctt?tgtctgcttc?gttgttgctt 180
acgaaagttt?caacttgttt?catagcagtg?cgcattgcag?atttgattgt?tgcgttgtgc 240
gcgcggcgct?cgttgtttgt?ttttgtgcgt?ttgatcgctg?atttaatgtt?tggcaatgtg 300
tttcacctcc?taagacataa?cctctgttcc?ttttctatag?gttagatctc?aacaatagaa 360
caaattgtat?tctatcaaac?acacccttag?attgcaatat?aaatgtaaag?tatttttatt 420
tgaaagctcc?gcttcgcggg?aatgtttgca?gaccttgtgg?gcacactaac?accagttatg 480
tttttggagg?gaatgcaaat?atg?gag?aaa?aag?aag?ctc?gat?tta?agt?caa?tat 533
Met?Glu?Lys?Lys?Lys?Leu?Asp?Leu?Ser?Gln?Tyr
1 5 10
gca?gtc?cgg?aca?gac?tta?gcc?gtg?gaa?gcg?agg?gat?ctg?gca?gag?gaa 581
Ala?Val?Arg?Thr?Asp?Leu?Ala?Val?Glu?Ala?Arg?Asp?Leu?Ala?Glu?Glu
15 20 25
aag?gaa?gct?tct?cca?aag?aaa?gag?ctg?aaa?ggg?ttt?aca?gtc?aaa?gaa 629
Lys?Glu?Ala?Ser?Pro?Lys?Lys?Glu?Leu?Lys?Gly?Phe?Thr?Val?Lys?Glu
30 35 40
tac?gaa?aaa?gac?ggg?atc?aaa?att?cag?acg?atg?gat?att?gat?gaa?gaa 677
Tyr?Glu?Lys?Asp?Gly?Ile?Lys?Ile?Gln?Thr?Met?Asp?Ile?Asp?Glu?Glu
45 50 55
gga?gcg?aag?ctg?tcg?ggg?aaa?aaa?gcc?ggc?cgc?tat?tta?acc?ttt?gaa 725
Gly?Ala?Lys?Leu?Set?Gly?Lys?Lys?Ala?Gly?Arg?Tyr?Leu?Thr?Phe?Glu
60 65 70 75
aca?caa?ggg?atc?aga?cag?cag?gat?tcc?gta?ttg?cag?gaa?aaa?gtc?gtc 773
Thr?Gln?Gly?Ile?Arg?Gln?Gln?Asp?Ser?Val?Leu?Gln?Glu?Lys?Val?Val
80 85 90
gat?gtc?ttc?gca?aag?gaa?ttt?tcc?tcg?ttt?ctc?gat?tat?ttg?ggc?att 821
Asp?Val?Phe?Ala?Lys?Glu?Phe?Ser?Ser?Phe?Leu?Asp?Tyr?Leu?Gly?Ile
95 100 105
ccg?cgg?gat?gcc?agc?tgc?tta?ata?gtc?ggg?ctc?ggc?aac?tgg?aat?gtc 869
Pro?Arg?Asp?Ala?Ser?Cys?Leu?Ile?Val?Gly?Leu?Gly?Asn?Trp?Asn?Val
110 115 120
acg?ccc?gac?tcg?ctc?ggc?ccc?cta?gtg?acg?gag?aac?ctt?ctc?gtg?acg 917
Thr?Pro?Asp?Ser?Leu?Gly?Pro?Leu?Val?Thr?Glu?Asn?Leu?Leu?Val?Thr
125 130 135
agg?cac?ctg?ttt?cag?ctg?cag?ccc?gaa?aat?gta?gag?gaa?ggc?tac?cgt 965
Arg?His?Leu?Phe?Gln?Leu?Gln?Pro?Glu?Asn?Val?Glu?Glu?Gly?Tyr?Arg
140 145 150 155
ccg?gtg?agt?gcg?ctt?tcc?ccg?gga?gtc?atg?ggc?ttg?acc?gga?att?gaa 1013
Pro?Val?Ser?Ala?Leu?Ser?Pro?Gly?Val?Met?Gly?Leu?Thr?Gly?Ile?Glu
160 165 170
acg?agc?gac?att?atc?caa?ggg?gtg?atc?gat?cgc?tca?aag?ccc?gat?ttt 1061
Thr?Ser?Asp?Ile?Ile?Gln?Gly?Val?Ile?Asp?Arg?Ser?Lys?Pro?Asp?Phe
175 180 185
gtc?att?gcc?att?gat?gcg?ctg?gca?tca?aga?ggg?atc?gag?cgt?gtg?aat 1109
Val?Ile?Ala?Ile?Asp?Ala?Leu?Ala?Ser?Arg?Gly?Ile?Glu?Arg?Val?Asn
190 195 200
tct?acc?att?caa?att?tca?gac?agc?ggg?ata?cac?ccc?ggg?tct?ggt?gtg 1157
Ser?Thr?Ile?Gln?Ile?Ser?Asp?Ser?Gly?Ile?His?Pro?Gly?Ser?Gly?Val
205 210 215
ggg?aat?aaa?aga?aaa?gag?tta?agc?aag?gat?acg?ctc?ggc?att?ccg?gtc 1205
Gly?Asn?Lys?Arg?Lys?Glu?Leu?Ser?Lys?Asp?Thr?Leu?Gly?Ile?Pro?Val
220 225 230 235
atc?gcc?atc?ggt?gtt?ccg?aca?gtg?gtc?gat?gcc?gtc?acg?atc?acg?agc 1253
Ile?Ala?Ile?Gly?Val?Pro?Thr?Val?Val?Asp?Ala?Val?Thr?Ile?Thr?Ser
240 245 250
gac?acg?att?gat?tac?atg?ctg?aag?cat?ttt?gga?aga?gag?atg?aga?gac 1301
Asp?Thr?Ile?Asp?Tyr?Met?Leu?Lys?His?Phe?Gly?Arg?Glu?Met?Arg?Asp
255 260 265
gac?agt?ccg?tcc?agg?tcg?ctc?gtt?ccc?gcg?ggg?atg?agc?ttt?ggg?aaa 1349
Asp?Ser?Pro?Ser?Arg?Ser?Leu?Val?Pro?Ala?Gly?Met?Ser?Phe?Gly?Lys
270 275 280
aga?aag?gtc?ctc?acc?gaa?gaa?gac?ctt?ccc?gat?gaa?gag?cac?cgc?aaa 1397
Arg?Lys?Val?Leu?Thr?Glu?Glu?Asp?Leu?Pro?Asp?Glu?Glu?His?Arg?Lys
285 290 295
tcg?ttt?ctc?ggt?ata?gtc?gga?ggc?ctc?gca?gag?gat?gaa?aaa?agg?cag 1445
Ser?Phe?Leu?Gly?Ile?Val?Gly?Gly?Leu?Ala?Glu?Asp?Glu?Lys?Arg?Gln
300 305 310 315
ctg?atc?cat?gaa?gtc?ctc?gct?ccg?ctc?ggc?cat?aac?tta?atg?gtg?acc 1493
Leu?Ile?His?Glu?Val?Leu?Ala?Pro?Leu?Gly?His?Asn?Leu?Met?Val?Thr
320 325 330
cct?aaa?gag?gtc?gat?aca?ttt?atc?gat?gat?atg?gca?aat?gtg?att?gca 1541
Pro?Lys?Glu?Val?Asp?Thr?Phe?Ile?Asp?Asp?Met?Ala?Asn?Val?Ile?Ala
335 340 345
aac?ggt?ctg?aac?aca?gcg?ctc?cat?gaa?aat?gta?tcg?caa?gac?aat?aaa 1589
Asn?Gly?Leu?Asn?Thr?Ala?Leu?His?Glu?Asn?Val?Ser?Gln?Asp?Asn?Lys
350 355 360
gga?atg?tat?aac?cac?taagatcaag?gcggctggcc?cggccgcttt?ttttcatgac 1644
Gly?Met?Tyr?Asn?His
365
atttagcatt?gccggaactt?gttctacttc?ctctatcttg?tacatagtct?atttactaga 1704
caagctctgg?agggatttac?aatgagaaaa?agaggcagga?atcgtcagtt?tgtgctggct l764
gtaaatggaa?gaagcgcggt?taagacagta?ttcttattta?tcgtcagcct?gctgctcgtt 1824
tttattttat?caggcgtgct?cacatcgctg?cgtcctgagt?taaggccgtc?ttcatcgttg 1884
taccgggtgg?ctgatgaact?gaagggcgag?acctttgggc?tcattttggg?aatggaaaac 1944
cactactttg?catcagaatt?gccggaaccg?aataagcgct?ttgagctttc?ccccctcgtc 2004
ctgaagctgg?cgaccagcat?taatttgaaa?gatccgcgaa?gttttctcgg?acgggagctt 2064
ccgggattct?ctcattttga?ctcggaaatc?ctcattgccg?ggc 2107
<2l0>26
<211>368
<212>PRT
＜213〉Bacillus licheniformis
<400>26
Met?Glu?Lys?Lys?Lys?Leu?Asp?Leu?Ser?Gln?Tyr?Ala?Val?Arg?Thr?Asp
1 5 10 15
Leu?Ala?Val?Glu?Ala?Arg?Asp?Leu?Ala?Glu?Glu?Lys?Glu?Ala?Ser?Pro
20 25 30
Lys?Lys?Glu?Leu?Lys?Gly?Phe?Thr?Val?Lys?Glu?Tyr?Glu?Lys?Asp?Gly
35 40 45
Ile?Lys?Ile?Gln?Thr?Met?Asp?Ile?Asp?Glu?Glu?Gly?Ala?Lys?Leu?Ser
50 55 60
Gly?Lys?Lys?Ala?Gly?Arg?Tyr?Leu?Thr?Phe?Glu?Thr?Gln?Gly?Ile?Arg
65 70 75 80
Gln?Gln?Asp?Ser?Val?Leu?Gln?Glu?Lys?Val?Val?Asp?Val?Phe?Ala?Lys
85 90 95
Glu?Phe?Ser?Ser?Phe?Leu?Asp?Tyr?Leu?Gly?Ile?Pro?Arg?Asp?Ala?Ser
100 105 110
Cys?Leu?Ile?Val?Gly?Leu?Gly?Asn?Trp?Asn?Val?Thr?Pro?Asp?Ser?Leu
115 120 125
Gly?Pro?Leu?Val?Thr?Glu?Asn?Leu?Leu?Val?Thr?Arg?His?Leu?Phe?Gln
130 135 140
Leu?Gln?Pro?Glu?Asn?Val?Glu?Glu?Gly?Tyr?Arg?Pro?Val?Ser?Ala?Leu
145 150 155 160
Ser?Pro?Gly?Val?Met?Gly?Leu?Thr?Gly?Ile?Glu?Thr?Ser?Asp?Ile?Ile
165 170 175
Gln?Gly?Val?Ile?Asp?Arg?Ser?Lys?Pro?Asp?Phe?Val?Ile?Ala?Ile?Asp
180 185 190
Ala?Leu?Ala?Ser?Arg?Gly?Ile?Glu?Arg?Val?Asn?Ser?Thr?Ile?Gln?Ile
195 200 205
Ser?Asp?Ser?Gly?Ile?His?Pro?Gly?Ser?Gly?Val?Gly?Asn?Lys?Arg?Lys
210 215 220
Glu?Leu?Ser?Lys?Asp?Thr?Leu?Gly?Ile?Pro?Val?Ile?Ala?Ile?Gly?Val
225 230 235 240
Pro?Thr?Val?Val?Asp?Ala?Val?Thr?Ile?Thr?Ser?Asp?Thr?Ile?Asp?Tyr
245 250 255
Met?Leu?Lys?His?Phe?Gly?Arg?Glu?Met?Arg?Asp?Asp?Ser?Pro?Ser?Arg
260 265 270
Ser?Leu?Val?Pro?Ala?Gly?Met?Ser?Phe?Gly?Lys?Arg?Lys?Val?Leu?Thr
275 280 285
Glu?Glu?Asp?Leu?Pro?Asp?Glu?Glu?His?Arg?Lys?Ser?Phe?Leu?Gly?Ile
290 295 300
Val?Gly?Gly?Leu?Ala?Glu?Asp?Glu?Lys?Arg?Gln?Leu?Ile?His?Glu?Val
305 310 315 320
Leu?Ala?Pro?Leu?Gly?His?Asn?Leu?Met?Val?Thr?Pro?Lys?Glu?Val?Asp
325 330 335
Thr?Phe?Ile?Asp?Asp?Met?Ala?Asn?Val?Ile?Ala?Asn?Gly?Leu?Asn?Thr
340 345 350
Ala?Leu?His?Glu?Asn?Val?Ser?Gln?Asp?Asn?Lys?Gly?Met?Tyr?Asn?His
355 360 365
<210>27
<211>2358
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1868)
<400>27
tgtcaatgtc?ggcatcgcgc?gcagaaataa?aaatgacggg?gacttccgga?tcttgatttt 60
ttatttcttt?tattagcgta?tacccgtcga?tatctggtaa?catgatatcg?agaatccata 120
aatgaggtga?ctggcctatt?ttttgcctgg?cgctttcacc?gtttagaaaa?gaagtgacgt 180
gccagccttc?actctctaag?tattttgtca?gcaattcatt?taaattctct?tcatcttcaa 240
ctagataaat?ggagtatgac?aagggcggtt?cacatccttt?ctacgtcatt?atactagttt 300
taacataagt?ctggtcaatt?ttcataattt?cacatattct?tttcattttt?atcccacaat 360
gttttcgtaa?catattcatc?agggaagaaa?gggaatgaaa?acagagtgaa?cgatggccgg 420
gtctcttttt?ttgtggatgt?taaagcttta?atgccatttg?cgatagttga?agacaagaaa 480
aataaaggaa?gtgtacgctt?atg?gat?ttt?aga?cgc?gat?gac?gaa?caa?aaa?cat 533
Met?Asp?Phe?Arg?Arg?Asp?Asp?Glu?Gln?Lys?His
1 5 10
gca?aat?gaa?gag?cag?ttt?cac?gaa?gaa?gcg?cag?acg?ccg?caa?acg?gca 581
Ala?Asn?Glu?Glu?Gln?Phe?His?Glu?Glu?Ala?Gln?Thr?Pro?Gln?Thr?Ala
15 20 25
ggt?tct?gag?gag?cag?cag?tca?gaa?cag?ccg?gca?aag?gag?ccc?gag?ctg 629
Gly?Ser?Glu?Glu?Gln?Gln?Ser?Glu?Gln?Pro?Ala?Lys?Glu?Pro?Glu?Leu
30 35 40
att?tta?aag?aag?gat?aca?gac?gat?cag?tca?gat?ctt?acg?aat?aaa?gaa 677
Ile?Leu?Lys?Lys?Asp?Thr?Asp?Asp?Gln?Ser?Asp?Leu?Thr?Asn?Lys?Glu
45 50 55
acc?gct?gca?gcc?ctg?gaa?gat?gcc?agg?gaa?acg?aga?gcg?gca?aaa?gaa 725
Thr?Ala?Ala?Ala?Leu?Glu?Asp?Ala?Arg?Glu?Thr?Arg?Ala?Ala?Lys?Glu
60 65 70 75
aag?cgg?cgc?aag?gct?tcg?tgg?ctg?agc?ccg?att?ttg?ggc?ggg?atc?atc 773
Lys?Arg?Arg?Lys?Ala?Ser?Trp?Leu?Ser?Pro?Ile?Leu?Gly?Gly?Ile?Ile
80 85 90
gga?ggc?ggc?ttg?gtg?ctt?gga?atc?tcc?ccg?tat?ctg?ccg?ggc?gat?cat 821
Gly?Gly?Gly?Leu?Val?Leu?Gly?Ile?Ser?Pro?Tyr?Leu?Pro?Gly?Asp?His
95 100 105
ccg?agc?gaa?act?gcc?gga?acc?gga?cag?aca?gag?cca?aag?cag?tcg?cag 869
Pro?Ser?Glu?Thr?Ala?Gly?Thr?Gly?Gln?Thr?Glu?Pro?Lys?Gln?Ser?Gln
110 115 120
aat?ttc?acc?aca?aaa?cct?gtc?acg?aac?gca?gat?aac?gta?cct?gat?atg 917
Asn?Phe?Thr?Thr?Lys?Pro?Val?Thr?Asn?Ala?Asp?Asn?Val?Pro?Asp?Met
125 130 135
gtg?gaa?gat?ttg?gag?cct?gcg?atc?gtc?gga?gtt?tca?aat?att?caa?acg 965
Val?Glu?Asp?Leu?Glu?Pro?Ala?Ile?Val?Gly?Val?Ser?Asn?Ile?Gln?Thr
140 145 150 155
agc?ttc?ggc?ttc?tct?gaa?gac?gac?gtg?gag?gag?agc?ggc?acg?gga?tca 1013
Ser?Phe?Gly?Phe?Ser?Glu?Asp?Asp?Val?Glu?Glu?Ser?Gly?Thr?Gly?Ser
160 165 170
ggc?gtc?atc?ttt?aag?aaa?gac?ggc?ggc?aag?gcc?tac?att?atc?aca?aac 1061
Gly?Val?Ile?Phe?Lys?Lys?Asp?Gly?Gly?Lys?Ala?Tyr?Ile?Ile?Thr?Asn
175 180 185
aac?cat?gtc?gtt?gaa?ggc?gcc?tcg?aag?gtg?acc?atc?tcc?tta?tac?aac 1109
Asn?His?Val?Val?Glu?Gly?Ala?Ser?Lys?Val?Thr?Ile?Ser?Leu?Tyr?Asn
190 195 200
gga?aag?acc?gcg?gat?gcc?aag?att?atc?ggc?agc?gac?gct?ttg?act?gat 1157
Gly?Lys?Thr?Ala?Asp?Ala?Lys?Ile?Ile?Gly?Ser?Asp?Ala?Leu?Thr?Asp
205 210 215
ctg?gct?gtt?tta?gag?atc?agc?agc?aaa?ggc?gtt?gat?aag?gtt?gca?agt 1205
Leu?Ala?Val?Leu?Glu?Ile?Ser?Ser?Lys?Gly?Val?Asp?Lys?Val?Ala?Ser
220 225 230 235
ttc?gga?gac?tcc?gca?aaa?ctg?cgc?gcc?ggc?gaa?aaa?gta?att?gcg?atc 1253
Phe?Gly?Asp?Ser?Ala?Lys?Leu?Arg?Ala?Gly?Glu?Lys?Val?Ile?Ala?Ile
240 245 250
ggg?aac?cct?ctc?ggc?ctt?caa?ttt?tca?aga?acg?gtt?aca?gaa?ggc?atc 1301
Gly?Asn?Pro?Leu?Gly?Leu?Gln?Phe?Ser?Arg?Thr?Val?Thr?Glu?Gly?Ile
255 260 265
atc?agc?ggt?gtc?aac?cgg?acg?att?gaa?gtc?tca?aca?tct?gaa?ggg?aat 1349
Ile?Ser?Gly?Val?Asn?Arg?Thr?Ile?Glu?Val?Ser?Thr?Ser?Glu?Gly?Asn
270 275 280
tgg?gat?atg?aac?gtc?ctt?cag?aca?gat?gcg?gcg?atc?aac?cct?gga?aac 1397
Trp?Asp?Met?Asn?Val?Leu?Gln?Thr?Asp?Ala?Ala?Ile?Asn?Pro?Gly?Asn
285 290 295
agc?ggc?ggg?cct?ctg?att?aac?agc?agc?ggg?cag?gtc?atc?ggc?atc?aac 1445
Ser?Gly?Gly?Pro?Leu?Ile?Asn?Ser?Ser?Gly?Gln?Val?Ile?Gly?Ile?Asn
300 305 310 315
agc?ctg?aag?atc?agc?caa?agc?ggt?gtt?gaa?tcg?ctt?ggc?ttc?gcg?att 1493
Ser?Leu?Lys?Ile?Ser?Gln?Ser?Gly?Val?Glu?Ser?Leu?Gly?Phe?Ala?Ile
320 325 330
ccg?agc?aac?gat?gtt?cag?ccg?atc?gtt?gac?gag?ctt?ttg?gaa?aaa?ggg 1541
Pro?Ser?Asn?Asp?Val?Gln?Pro?Ile?Val?Asp?Glu?Leu?Leu?Glu?Lys?Gly
335 340 345
aaa?gtg?gaa?cgg?cct?ttc?ctc?ggc?gtg?caa?atg?atc?gat?atg?cag?cag 1589
Lys?Val?Glu?Arg?Pro?Phe?Leu?Gly?Val?Gln?Met?Ile?Asp?Met?Gln?Gln
350 355 360
gtg?cct?gaa?cag?tac?cag?caa?aat?acg?ctc?ggc?cta?ttc?gga?gac?cag 1637
Val?Pro?Glu?Gln?Tyr?Gln?Gln?Asn?Thr?Leu?Gly?Leu?Phe?Gly?Asp?Gln
365 370 375
ctt?aat?aaa?ggc?gtg?tac?atc?gat?aaa?gta?tcg?ccg?aaa?tcg?cct?gcg 1685
Leu?Asn?Lys?Gly?Val?Tyr?Ile?Asp?Lys?Val?Ser?Pro?Lys?Ser?Pro?Ala
380 385 390 395
gca?gac?gcg?ggt?atg?aaa?gcc?ggc?gac?gtc?atc?acc?aaa?atg?aac?ggc 1733
Ala?Asp?Ala?Gly?Met?Lys?Ala?Gly?Asp?Val?Ile?Thr?Lys?Met?Asn?Gly
400 405 410
aaa?aac?gtc?gaa?acc?acc?tca?gac?ctc?aga?aaa?att?ctt?tat?aca?gaa 1781
Lys?Asn?Val?Glu?Thr?Thr?Ser?Asp?Leu?Arg?Lys?Ile?Leu?Tyr?Thr?Glu
415 420 425
gca?aaa?gca?gga?gat?act?gtc?aca?ttt?gag?gtg?ctg?aga?aac?gga?aag 1829
Ala?Lys?Ala?Gly?Asp?Thr?Val?Thr?Phe?Glu?Val?Leu?Arg?Asn?Gly?Lys
430 435 440
caa?aca?aca?atg?aaa?gca?aaa?ctt?gcg?aaa?agc?aaa?tca?taaataaaag 1878
Gln?Thr?Thr?Met?Lys?Ala?Lys?Leu?Ala?Lys?Ser?Lys?Ser
445 450 455
ccatggcaaa?cgccatggct?ttttcatatt?acttgtccaa?aaatgaagaa?agcatccaaa 1938
tctgcttatc?aatttcctct?gtcagggcga?tataaaggtc?agcggtagaa?tgatctgatt 1998
tatcttccgc?aagttcgatg?gtatgctgaa?tttcatcgcg?gatttgacgg?taatcgctta 2058
ccagtgcgga?aaccatttct?tcggccgttt?tttcggctcc?gttttcctca?attgtgccgt 2118
ggtcaatgta?ttctttcatt?gtggcgagcg?gctgtccgcc?gatggccagc?aagcgttccg 2178
cgatcacatc?tgctgtttct?gacgcatggt?tgtatagctc?ttcaaatttt?tcatgtaggg 2238
taaagaactg?cggccctttt?acgtaccagt?gaaaacggtg?aagcttcgtg?taaaggatga 2298
accagtttga?caaattcgta?ttcattgagt?tttcaagtac?aggatttgct?gttttaattt 2358
<210>28
<211>456
<212>PRT
＜213〉Bacillus licheniformis
<400>28
Met?Asp?Phe?Arg?Arg?Asp?Asp?Glu?Gln?Lys?His?Ala?Asn?Glu?Glu?Gln
1 5 10 15
Phe?His?Glu?Glu?Ala?Gln?Thr?Pro?Gln?Thr?Ala?Gly?Ser?Glu?Glu?Gln
20 25 30
Gln?Ser?Glu?Gln?Pro?Ala?Lys?Glu?Pro?Glu?Leu?Ile?Leu?Lys?Lys?Asp
35 40 45
Thr?Asp?Asp?Gln?Ser?Asp?Leu?Thr?Asn?Lys?Glu?Thr?Ala?Ala?Ala?Leu
50 55 60
Glu?Asp?Ala?Arg?Glu?Thr?Arg?Ala?Ala?Lys?Glu?Lys?Arg?Arg?Lys?Ala
65 70 75 80
Ser?Trp?Leu?Ser?Pro?Ile?Leu?Gly?Gly?Ile?Ile?Gly?Gly?Gly?Leu?Val
85 90 95
Leu?Gly?Ile?Ser?Pro?Tyr?Leu?Pro?Gly?Asp?His?Pro?Ser?Glu?Thr?Ala
100 105 110
Gly?Thr?Gly?Gln?Thr?Glu?Pro?Lys?Gln?Ser?Gln?Asn?Phe?Thr?Thr?Lys
115 120 125
Pro?Val?Thr?Asn?Ala?Asp?Asn?Val?Pro?Asp?Met?Val?Glu?Asp?Leu?Glu
130 135 140
Pro?Ala?Ile?Val?Gly?Val?Ser?Asn?Ile?Gln?Thr?Ser?Phe?Gly?Phe?Ser
145 150 155 160
Glu?Asp?Asp?Val?Glu?Glu?Ser?Gly?Thr?Gly?Ser?Gly?Val?Ile?Phe?Lys
165 170 175
Lys?Asp?Gly?Gly?Lys?Ala?Tyr?Ile?Ile?Thr?Asn?Asn?His?Val?Val?Glu
180 185 190
Gly?Ala?Ser?Lys?Val?Thr?Ile?Ser?Leu?Tyr?Asn?Gly?Lys?Thr?Ala?Asp
195 200 205
Ala?Lys?Ile?Ile?Gly?Ser?Asp?Ala?Leu?Thr?Asp?Leu?Ala?Val?Leu?Glu
210 215 220
Ile?Ser?Ser?Lys?Gly?Val?Asp?Lys?Val?Ala?Ser?Phe?Gly?Asp?Ser?Ala
225 230 235 240
Lys?Leu?Arg?Ala?Gly?Glu?Lys?Val?Ile?Ala?Ile?Gly?Asn?Pro?Leu?Gly
245 250 255
Leu?Gln?Phe?Ser?Arg?Thr?Val?Thr?Glu?Gly?Ile?Ile?Ser?Gly?Val?Asn
260 265 270
Arg?Thr?Ile?Glu?Val?Ser?Thr?Ser?Glu?Gly?Asn?Trp?Asp?Met?Asn?Val
275 280 285
Leu?Gln?Thr?Asp?Ala?Ala?Ile?Asn?Pro?Gly?Asn?Ser?Gly?Gly?Pro?Leu
290 295 300
Ile?Asn?Ser?Ser?Gly?Gln?Val?Ile?Gly?Ile?Asn?Ser?Leu?Lys?Ile?Ser
305 310 315 320
Gln?Ser?Gly?Val?Glu?Ser?Leu?Gly?Phe?Ala?Ile?Pro?Ser?Asn?Asp?Val
325 330 335
Gln?Pro?Ile?Val?Asp?Glu?Leu?Leu?Glu?Lys?Gly?Lys?Val?Glu?Arg?Pro
340 345 350
Phe?Leu?Gly?Val?Gln?Met?Ile?Asp?Met?Gln?Gln?Val?Pro?Glu?Gln?Tyr
355 360 365
Gln?Gln?Asn?Thr?Leu?Gly?Leu?Phe?Gly?Asp?Gln?Leu?Asn?Lys?Gly?Val
370 375 380
Tyr?Ile?Asp?Lys?Val?Ser?Pro?Lys?Ser?Pro?Ala?Ala?Asp?Ala?Gly?Met
385 390 395 400
Lys?Ala?Gly?Asp?Val?Ile?Thr?Lys?Met?Asn?Gly?Lys?Asn?Val?Glu?Thr
405 410 415
Thr?Ser?Asp?Leu?Arg?Lys?Ile?Leu?Tyr?Thr?Glu?Ala?Lys?Ala?Gly?Asp
420 425 430
Thr?Val?Thr?Phe?Glu?Val?Leu?Arg?Asn?Gly?Lys?Gln?Thr?Thr?Met?Lys
435 440 445
Ala?Lys?Leu?Ala?Lys?Ser?Lys?Ser
450 455
<210>29
<211>1647
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1559)
<400>29
cactgtaagt?ggacattttt?ttgcgtgatg?agaaggggga?ggcagagttg?ccgcatattt 60
taattttgaa?cggaccgaat?ttaaaccggc?ttggaaaacg?cgaacccgat?gtatacggga 120
cagacacatt?aaccgatttg?gaacaaagac?tttttcaatt?tgccgaaggc?attcagacag 180
aattgacttt?ttttcagtca?aaccacgaag?gggacctgat?tgatgctctt?catgaggcag 240
aggagcaata?cgatggaatc?gtcttgaatc?cgggggcttt?ctcgcattac?agctatgccc 300
tccgcgatgc?ggtggctgca?atcagcatac?cggtgattga?ggttcatctt?tcaaatcctc 360
atgcaaggga?agagttccgg?caccgctcgg?tcatcgctcc?ggttgcaaga?ggccagatca 420
ccggactcgg?ctttgaaggt?tataagctgg?ccatctctta?ttttatgaac?acaaataaca 480
aataggggga?ctagtggaca?atg?aaa?cta?aac?aaa?ttg?aga?gag?ctt?ttc?ggc 533
Met?Lys?Leu?Asn?Lys?Leu?Arg?Glu?Leu?Phe?Gly
1 5 10
ggt?tta?ggc?att?gac?ggt?ata?ttg?gtg?acg?agc?ggc?gtt?aat?ttg?cgg 581
Gly?Leu?Gly?Ile?Asp?Gly?Ile?Leu?Val?Thr?Ser?Gly?Val?Asn?Leu?Arg
15 20 25
tac?atc?acc?gga?ttt?acc?gga?tct?tca?gga?ctg?gcg?gtc?atc?tca?gac 629
Tyr?Ile?Thr?Gly?Phe?Thr?Gly?Ser?Ser?Gly?Leu?Ala?Val?Ile?Ser?Asp
30 35 40
gat?caa?gca?gtc?ttt?att?acg?gat?ttc?cgc?tat?aca?gaa?cag?gcg?aag 677
Asp?Gln?Ala?Val?Phe?Ile?Thr?Asp?Phe?Arg?Tyr?Thr?Glu?Gln?Ala?Lys
45 50 55
gat?caa?atc?aaa?agc?ttt?gac?atc?gtt?cag?cat?gcc?ggc?gga?atc?gtt 725
Asp?Gln?Ile?Lys?Ser?Phe?Asp?Ile?Val?Gln?His?Ala?Gly?Gly?Ile?Val
60 65 70 75
caa?aag?acc?gcc?gaa?gtg?atc?aaa?gag?atg?ggg?ata?aac?aaa?atc?ggt 773
Gln?Lys?Thr?Ala?Glu?Val?Ile?Lys?Glu?Met?Gly?Ile?Asn?Lys?Ile?Gly
80 85 90
ttt?gaa?cag?gat?aaa?atg?aca?tac?gga?acg?tat?gcc?gcc?tac?aaa?gag 821
Phe?Glu?Gln?Asp?Lys?Met?Thr?Tyr?Gly?Thr?Tyr?Ala?Ala?Tyr?Lys?Glu
95 100 105
cag?ctt?ggc?gca?gct?gaa?ctc?gtt?ccc?gtt?tct?caa?tct?gtg?gaa?aag 869
Gln?Leu?Gly?Ala?Ala?Glu?Leu?Val?Pro?Val?Ser?Gln?Ser?Val?Glu?Lys
110 115 120
ttg?cgc?ttg?att?aag?tca?agt?gaa?gag?att?aag?ata?tta?aag?gaa?gct 917
Leu?Arg?Leu?Ile?Lys?Ser?Ser?Glu?Glu?Ile?Lys?Ile?Leu?Lys?Glu?Ala
125 130 135
gca?aag?att?gca?gat?gat?gca?ttc?agc?cat?att?ctg?acg?gtc?atc?aag 965
Ala?Lys?Ile?Ala?Asp?Asp?Ala?Phe?Ser?His?Ile?Leu?Thr?Val?Ile?Lys
140 145 150 155
ccc?ggc?atc?acc?gaa?att?gaa?gtc?gcc?aat?gag?ctc?gag?ttt?tac?atg 1013
Pro?Gly?Ile?Thr?Glu?Ile?Glu?Val?Ala?Asn?Glu?Leu?Glu?Phe?Tyr?Met
160 165 170
cgg?agc?cag?gga?gcc?gat?cat?tcg?tcc?ttc?gat?atg?atc?gtg?gcg?tca 1061
Arg?Ser?Gln?Gly?Ala?Asp?His?Ser?Ser?Phe?Asp?Met?Ile?Val?Ala?Ser
175 180 185
ggc?gta?cgc?tca?agt?ctg?ccg?cac?gga?gtg?gca?agc?ggc?aaa?gcg?atc 1109
Gly?Val?Arg?Ser?Ser?Leu?Pro?His?Gly?Val?Ala?Ser?Gly?Lys?Ala?Ile
190 195 200
gag?aaa?ggc?gac?ctt?gtg?acg?ctg?gac?ttt?ggg?gct?tac?tat?aaa?ggt 1157
Glu?Lys?Gly?Asp?Leu?Val?Thr?Leu?Asp?Phe?Gly?Ala?Tyr?Tyr?Lys?Gly
205 210 215
tac?tgc?tcg?gac?att?acg?cgt?aca?gtc?gcg?gta?gga?gag?ccg?gat?gat 1205
Tyr?Cys?Ser?Asp?Ile?Thr?Arg?Thr?Val?Ala?Val?Gly?Glu?Pro?Asp?Asp
220 225 230 235
gag?ctg?aaa?cgc?att?tat?caa?acc?gtg?ttt?gag?gcg?cag?gct?atc?ggc 1253
Glu?Leu?Lys?Arg?Ile?Tyr?Gln?Thr?Val?Phe?Glu?Ala?Gln?Ala?Ile?Gly
240 245 250
atg?cgg?agc?atc?aag?ccg?gga?atc?acc?ggg?aaa?cag?gct?gac?gca?tac 1301
Met?Arg?Ser?Ile?Lys?Pro?Gly?Ile?Thr?Gly?Lys?Gln?Ala?Asp?Ala?Tyr
255 260 265
aca?agg?gac?tac?ata?tct?tca?caa?ggc?tac?ggc?gat?tat?ttc?ggc?cat 1349
Thr?Arg?Asp?Tyr?Ile?Ser?Ser?Gln?Gly?Tyr?Gly?Asp?Tyr?Phe?Gly?His
270 275 280
tca?aca?ggc?cat?ggc?ctc?ggc?atg?gaa?gtt?cat?gaa?agt?ecg?gcg?ctt 1397
Ser?Thr?Gly?His?Gly?Leu?Gly?Met?Glu?Val?His?Glu?Ser?Pro?Ala?Leu
285 290 295
tca?gca?cgt?tct?gac?cag?atg?ctt?gaa?aaa?ggc?atg?gtt?gta?acg?gtt 1445
Ser?Ala?Arg?Ser?Asp?Gln?Met?Leu?Glu?Lys?Gly?Met?Val?Val?Thr?Val
300 305 310 315
gaa?ccg?gga?att?tac?ata?ccg?ggc?aaa?ggc?ggg?gtc?agg?ata?gaa?gat 1493
Glu?Pro?Gly?Ile?Tyr?Ile?Pro?Gly?Lys?Gly?Gly?Val?Arg?Ile?Glu?Asp
320 325 330
gat?att?gtc?ctg?aca?gag?gaa?gga?aac?gaa?tcc?ctt?acc?cat?tcc?gcg 1541
Asp?Ile?Val?Leu?Thr?Glu?Glu?Gly?Asn?Glu?Ser?Leu?Thr?His?Ser?Ala
335 340 345
aaa?gat?tta?att?att?tta?taatccgttt?gattggagca?ataggaggat 1589
Lys?Asp?Leu?Ile?Ile?Leu
350
aatcatgatt?tcagtaaacg?atttcgcaca?gtttaagtat?caataaggga?gtttctaa 1647
<210>30
<211>353
<212>PRT
＜213〉Bacillus licheniformis
<400>30
Met?Lys?Leu?Asn?Lys?Leu?Arg?Glu?Leu?Phe?Gly?Gly?Leu?Gly?Ile?Asp
1 5 10 15
Gly?Ile?Leu?Val?Thr?Ser?Gly?Val?Asn?Leu?Arg?Tyr?Ile?Thr?Gly?Phe
20 25 30
Thr?Gly?Ser?Ser?Gly?Leu?Ala?Val?Ile?Ser?Asp?Asp?Gln?Ala?Val?Phe
35 40 45
Ile?Thr?Asp?Phe?Arg?Tyr?Thr?Glu?Gln?Ala?Lys?Asp?Gln?Ile?Lys?Ser
50 55 60
Phe?Asp?Ile?Val?Gln?His?Ala?Gly?Gly?Ile?Val?Gln?Lys?Thr?Ala?Glu
65 70 75 80
Val?Ile?Lys?Glu?Met?Gly?Ile?Asn?Lys?Ile?Gly?Phe?Glu?Gln?Asp?Lys
85 90 95
Met?Thr?Tyr?Gly?Thr?Tyr?Ala?Ala?Tyr?Lys?Glu?Gln?Leu?Gly?Ala?Ala
100 105 110
Glu?Leu?Val?Pro?Val?Ser?Gln?Ser?Val?Glu?Lys?Leu?Arg?Leu?Ile?Lys
115 120 125
Ser?Ser?Glu?Glu?Ile?Lys?Ile?Leu?Lys?Glu?Ala?Ala?Lys?Ile?Ala?Asp
130 135 140
Asp?Ala?Phe?Ser?His?Ile?Leu?Thr?Val?Ile?Lys?Pro?Gly?Ile?Thr?Glu
145 150 155 160
Ile?Glu?Val?Ala?Asn?Glu?Leu?Glu?Phe?Tyr?Met?Arg?Ser?Gln?Gly?Ala
165 170 175
Asp?His?Ser?Ser?Phe?Asp?Met?Ile?Val?Ala?Ser?Gly?Val?Arg?Ser?Ser
180 185 190
Leu?Pro?His?Gly?Val?Ala?Ser?Gly?Lys?Ala?Ile?Glu?Lys?Gly?Asp?Leu
195 200 205
Val?Thr?Leu?Asp?Phe?Gly?Ala?Tyr?Tyr?Lys?Gly?Tyr?Cys?Ser?Asp?Ile
210 215 220
Thr?Arg?Thr?Val?Ala?Val?Gly?Glu?Pro?Asp?Asp?Glu?Leu?Lys?Arg?Ile
225 230 235 240
Tyr?Gln?Thr?Val?Phe?Glu?Ala?Gln?Ala?Ile?Gly?Met?Arg?Ser?Ile?Lys
245 250 255
Pro?Gly?Ile?Thr?Gly?Lys?Gln?Ala?Asp?Ala?Tyr?Thr?Arg?Asp?Tyr?Ile
260 265 270
Ser?Ser?Gln?Gly?Tyr?Gly?Asp?Tyr?Phe?Gly?His?Ser?Thr?Gly?His?Gly
275 280 285
Leu?Gly?Met?Glu?Val?His?Glu?Ser?Pro?Ala?Leu?Ser?Ala?Arg?Ser?Asp
290 295 300
Gln?Met?Leu?Glu?Lys?Gly?Met?Val?Val?Thr?Val?Glu?Pro?Gly?Ile?Tyr
305 310 315 320
Ile?Pro?Gly?Lys?Gly?Gly?Val?Arg?Ile?Glu?Asp?Asp?Ile?Val?Leu?Thr
325 330 335
Glu?Glu?Gly?Asn?Glu?Ser?Leu?Thr?His?Ser?Ala?Lys?Asp?Leu?Ile?Ile
340 345 350
Leu
<210>31
<211>1941
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1451)
<400>31
gcaaaaaaat?cgaaaagccg?gaaattacaa?agctcacaga?catcatcgat?acagatttga 60
tcgaacctca?tttgtcagga?gatacgagag?acgacattat?tgatgaaatg?atccaaaaat 120
tgtcccggaa?aggcgtactg?cattcagaaa?gcgaatttaa?acaagctatc?atcaatcgcg 180
aacttgaggg?tacaacggcg?atcggtatga?atatcgcgat?cccgcacggg?aagtctgatg 240
ccgtcaagaa?gccgagtgta?gcattcggca?ttaaacgttc?gggcgtagac?tggaggagtc 300
ttgacggaac?tgacgccaaa?ctgattttta?tgatcgccgt?cccgaaagaa?agcggaggaa 360
acgagcatct?gaagcttctg?caaatgctct?ccagaaaact?gatggatgac?acctaccggg 420
acaagcttct?ctctgcccaa?acaaaagaag?aagcatacaa?acttctggat?gaaatcatct 480
aaaaaaggag?gacgcccgtc?atg?act?gaa?ccg?tta?ttt?ttc?gag?cct?gtt?ttt 533
Met?Thr?Glu?Pro?Leu?Phe?Phe?Glu?Pro?Val?Phe
1 5 10
aaa?gaa?aga?ata?tgg?ggc?gga?acg?gct?tta?gcc?tct?ttc?ggc?tat?gac 581
Lys?Glu?Arg?Ile?Trp?Gly?Gly?Thr?Ala?Leu?Ala?Ser?Phe?Gly?Tyr?Asp
15 20 25
att?cca?tcc?gaa?caa?aca?ggg?gag?tgc?tgg?gct?ttc?gcc?gca?cat?caa 629
Ile?Pro?Ser?Glu?Gln?Thr?Gly?Glu?Cys?Trp?Ala?Phe?Ala?Ala?His?Gln
30 35 40
aat?gga?caa?agc?gtc?gtg?aaa?aac?ggc?ttg?tat?aaa?ggg?ctc?aca?tta 677
Asn?Gly?Gln?Ser?Val?Val?Lys?Asn?Gly?Leu?Tyr?Lys?Gly?Leu?Thr?Leu
45 50 55
agc?gaa?tta?tgg?gat?gaa?cac?cgt?cat?tta?ttc?gga?cat?ata?aag?gga 725
Ser?Glu?Leu?Trp?Asp?Glu?His?Arg?His?Leu?Phe?Gly?His?Ile?Lys?Gly
60 65 70 75
gac?cgc?ttt?cct?ctt?ttg?aca?aaa?att?ttg?gac?gcc?gac?caa?gac?cta 773
Asp?Arg?Phe?Pro?Leu?Leu?Thr?Lys?Ile?Leu?Asp?Ala?Asp?Gln?Asp?Leu
80 85 90
tcc?gta?caa?gtg?cat?cca?aat?gat?gaa?tat?gca?aag?ctg?cac?gaa?aac 821
Ser?Val?Gln?Val?His?Pro?Asn?Asp?Glu?Tyr?Ala?Lys?Leu?His?Glu?Asn
95 100 105
ggg?gag?ctt?gga?aag?acg?gaa?tgc?tgg?tat?atc?att?gat?tgc?aag?gaa 869
Gly?Glu?Leu?Gly?Lys?Thr?Glu?Cys?Trp?Tyr?Ile?Ile?Asp?Cys?Lys?Glu
110 115 120
gat?gcg?gag?atc?att?tac?ggc?cac?cat?gcc?aag?acg?cgg?gaa?gaa?ctg 917
Asp?Ala?Glu?Ile?Ile?Tyr?Gly?His?His?Ala?Lys?Thr?Arg?Glu?Glu?Leu
125 130 135
gtc?tca?atg?atc?gaa?aac?gga?aaa?tgg?aat?gag?ctt?ttg?cgc?cgc?gtc 965
Val?Ser?Met?Ile?Glu?Asn?Gly?Lys?Trp?Asn?Glu?Leu?Leu?Arg?Arg?Val
140 145 150 155
aaa?gtg?aag?ccg?gga?gat?ttc?ttc?tat?gtg?ccg?agc?gga?acc?gtc?cat 1013
Lys?Val?Lys?Pro?Gly?Asp?Phe?Phe?Tyr?Val?Pro?Ser?Gly?Thr?Val?His
160 165 170
gcg?atc?gga?aaa?ggc?att?ttg?att?ttg?gag?acg?cag?cag?aat?tct?gac 1061
Ala?Ile?Gly?Lys?Gly?Ile?Leu?Ile?Leu?Glu?Thr?Gln?Gln?Asn?Ser?Asp
175 180 185
aca?acc?tac?aga?cta?tat?gat?tat?gac?aga?aaa?gac?gct?gaa?gga?aac 1109
Thr?Thr?Tyr?Arg?Leu?Tyr?Asp?Tyr?Asp?Arg?Lys?Asp?Ala?Glu?Gly?Asn
190 195 200
ctg?cgc?gag?ctc?cat?ttg?caa?aaa?agc?ata?gaa?gtg?atc?gac?gtt?ccg 1157
Leu?Arg?Glu?Leu?His?Leu?Gln?Lys?Ser?Ile?Glu?Val?Ile?Asp?Val?Pro
205 210 215
tct?tcc?cct?gat?cgc?gaa?acc?gtt?caa?tac?gaa?aca?tcg?ggc?gac?gtc 1205
Ser?Ser?Pro?Asp?Arg?Glu?Thr?Val?Gln?Tyr?Glu?Thr?Ser?Gly?Asp?Val
220 225 230 235
aag?act?gcc?gca?ctg?att?gat?tgt?cct?tat?ttc?tca?gta?gaa?aaa?tgg 1253
Lys?Thr?Ala?Ala?Leu?Ile?Asp?Cys?Pro?Tyr?Phe?Ser?Val?Glu?Lys?Trp
240 245 250
gat?gta?aaa?gga?tcg?gcc?tgt?ttc?aaa?cag?gat?aaa?ccg?ttt?ctt?ctt 1301
Asp?Val?Lys?Gly?Ser?Ala?Cys?Phe?Lys?Gln?Asp?Lys?Pro?Phe?Leu?Leu
255 260 265
gcc?agc?gtc?ata?gaa?ggg?gaa?ggg?cgc?atc?gca?tct?ggc?ggc?cat?aac 1349
Ala?Ser?Val?Ile?Glu?Gly?Glu?Gly?Arg?Ile?Ala?Ser?Gly?Gly?His?Asn
270 275 280
ttt?tca?ttt?aaa?aaa?gga?gat?cac?atg?ctg?ctg?ccc?tgg?ggc?ttt?gga 1397
Phe?Ser?Phe?Lys?Lys?Gly?Asp?His?Met?Leu?Leu?Pro?Trp?Gly?Phe?Gly
285 290 295
gaa?ttt?acg?ctc?gcc?ggc?cat?gcg?gaa?tgt?atc?gtc?tca?agc?tta?tca 1445
Glu?Phe?Thr?Leu?Ala?Gly?His?Ala?Glu?Cys?Ile?Val?Ser?Ser?Leu?Ser
300 305 310 315
att?gaa?taaacgatca?gcggcgccct?ttctgaacag?accataaaac?tatggtctgt 1501
Ile?Glu
tttatttttg?caaaaaaaat?aatctcgact?tgccaagacg?tgttcacaga?gggctgacaa 1561
tgcatattcc?caagctttca?caagtaaaat?agagtaaacc?aaaaaaacat?ttggaaatct 1621
tccaattatt?tgataagctg?atattaaaag?cttcatatct?ttcatcagct?gcactgcccg 1681
ggacttttcg?cggaacagct?gcttcatgaa?acaaaaaggc?ggtcctatgt?tccggcgtct 1741
ggcaagaaaa?gcctgctaaa?tgaccgaaac?aaccgtgccc?ttcataattg?gttggtcctt 1801
atctggatga?gaaaaggaga?cgatgattcg?tatcagcaaa?aatgtaagcg?gattcatcgt 1861
ctttttatct?cggtttatca?ctctttttag?cggaagcgag?gaggtaagca?atggggaaag 1921
caaaacttcg?atggaatgaa 1941
<210>32
<211>317
<212>PRT
＜213〉Bacillus licheniformis
<400>32
Met?Thr?Glu?Pro?Leu?Phe?Phe?Glu?Pro?Val?Phe?Lys?Glu?Arg?Ile?Trp
1 5 10 15
Gly?G1y?Thr?A1a?Leu?A1a?Ser?Phe?Gly?Tyr?Asp?Ile?Pro?Ser?G1u?Gln
20 25 30
Thr?Gly?Glu?Cys?Trp?Ala?Phe?Ala?A1a?His?G1n?Asn?Gly?Gln?Ser?Val
35 40 45
Val?Lys?Asn?G1y?Leu?Tyr?Lys?Gly?Leu?Thr?Leu?Ser?Glu?Leu?Trp?Asp
50 55 60
Glu?His?Arg?His?Leu?Phe?Gly?His?Ile?Lys?Gly?Asp?Arg?Phe?Pro?Leu
65 70 75 80
Leu?Thr?Lys?Ile?Leu?Asp?Ala?Asp?Gln?Asp?Leu?Ser?Val?Gln?Val?His
85 90 95
Pro?Asn?Asp?Glu?Tyr?Ala?Lys?Leu?His?Glu?Asn?Gly?Glu?Leu?Gly?Lys
100 105 110
Thr?Glu?Cys?Trp?Tyr?Ile?Ile?Asp?Cys?Lys?Glu?Asp?Ala?Glu?Ile?Ile
115 120 125
Tyr?Gly?His?His?Ala?Lys?Thr?Arg?Glu?Glu?Leu?Val?Ser?Met?Ile?Glu
130 135 140
Asn?Gly?Lys?Trp?Asn?Glu?Leu?Leu?Arg?Arg?Val?Lys?Val?Lys?Pro?Gly
145 150 155 160
Asp?Phe?Phe?Tyr?Val?Pro?Ser?Gly?Thr?Val?His?Ala?Ile?Gly?Lys?Gly
165 170 175
Ile?Leu?Ile?Leu?Glu?Thr?Gln?Gln?Asn?Ser?Asp?Thr?Thr?Tyr?Arg?Leu
180 185 190
Tyr?Asp?Tyr?Asp?Arg?Lys?Asp?Ala?Glu?Gly?Asn?Leu?Arg?Glu?Leu?His
195 200 205
Leu?Gln?Lys?Ser?Ile?Glu?Val?Ile?Asp?Val?Pro?Ser?Ser?Pro?Asp?Arg
210 215 220
Glu?Thr?Val?Gln?Tyr?Glu?Thr?Ser?Gly?Asp?Val?Lys?Thr?Ala?Ala?Leu
225 230 235 240
Ile?Asp?Cys?Pro?Tyr?Phe?Ser?Val?Glu?Lys?Trp?Asp?Val?Lys?Gly?Ser
245 250 255
Ala?Cys?Phe?Lys?Gln?Asp?Lys?Pro?Phe?Leu?Leu?Ala?Ser?Val?Ile?Glu
260 265 270
Gly?Glu?Gly?Arg?Ile?Ala?Ser?Gly?Gly?His?Asn?Phe?Ser?Phe?Lys?Lys
275 280 285
Gly?Asp?His?Met?Leu?Leu?Pro?Trp?Gly?Phe?Gly?Glu?Phe?Thr?Leu?Ala
290 295 300
Gly?His?Ala?Glu?Cys?Ile?Val?Ser?Ser?Leu?Ser?Ile?Glu
305 310 315
<210>33
<211>1747
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1244)
<400>33
tcatacattg?ataaaggtga?gcttgttccg?gatgaagtga?caattggtat?tgtcagggag 60
agacttggca?agaatgattg?tgacggaggt?tttcttctgg?acggatttcc?gagaacagtc 120
gctcaagctg?aagcacttga?ggaaattctt?aaaggacttg?gcaagtcgat?tgaccatgtc 180
atcaacattc?aagtcgataa?agatgcattg?atggaacgtc?tgacaggacg?cagaatctgc 240
aaaaactgcg?gtgcaactta?tcatttagtc?tttaatccgc?ctgcaaaaga?aaatgtatgc 300
gacaagtgtg?gaggagagct?ttatcagcgt?gaagacgata?atgaagctac?cgtatccacg 360
cggttagagg?tcaacatgaa?gcaaacccag?cctttactcg?atttctatga?agataaagga 420
tatctcgtaa?acattgacgg?gcagaagcac?atcaatgaag?tttacgctga?tataaaggag 480
cttctcggag?gccgggacaa?atg?att?att?tgt?aaa?act?ccc?cga?gaa?atc?gaa 533
Met?Ile?Ile?Cys?Lys?Thr?Pro?Arg?Glu?Ile?Glu
1 5 10
atc?atg?cgg?gag?gcc?gga?cga?att?gtc?gct?tta?act?cat?cag?gag?cta 581
Ile?Met?Arg?Glu?Ala?Gly?Arg?Ile?Val?Ala?Leu?Thr?His?Gln?Glu?Leu
15 20 25
aaa?aag?cac?att?aaa?ccc?ggc?atc?tcg?aca?aaa?gaa?ttg?gat caa?att 629
Lys?Lys?His?Ile?Lys?Pro?Gly?Ile?Ser?Thr?Lys?Glu?Leu?Asp?Gln?Ile
30 35 40
gcc?gaa?cgt?ttt?att?aca?aag?caa?ggt?gca?atc?cca?tct?ttt?aaa?ggg 677
Ala?Glu?Arg?Phe?Ile?Thr?Lys?Gln?Gly?Ala?Ile?Pro?Ser?Phe?Lys?Gly
45 50 55
tat?aat?ggt?ttt?cgc?ggg?agc?att?tgc?gtt?tcg?gtc?aac?gaa?gaa?ctc 725
Tyr?Asn?Gly?Phe?Arg?Gly?Ser?Ile?Cys?Val?Ser?Val?Asn?Glu?Glu?Leu
60 65 70 75
gtg?cac?ggt?att?ccg?gga?aaa?cga?gtc?ctt?cgc?gac?ggg?gac?atc?atc 773
Val?His?Gly?Ile?Pro?Gly?Lys?Arg?Val?Leu?Arg?Asp?Gly?Asp?Ile?Ile
80 85 90
agc?atc?gat?atc?ggt?gcg?aag?cta?aac?ggt?tat?cat?ggt?gac?tcc?gct 821
Ser?Ile?Asp?Ile?Gly?Ala?Lys?Leu?Asn?Gly?Tyr?His?Gly?Asp?Ser?Ala
95 100 105
tgg?act?tat?cca?gtg?ggt?acg?atc?agc?gat?gat?gat?cag?aag?ctg?ctg 869
Trp?Thr?Tyr?Pro?Val?Gly?Thr?Ile?Ser?Asp?Asp?Asp?Gln?Lys?Leu?Leu
110 115 120
gat?gtg?aca?gag?gag?tct?tta?tat?aga?ggc?ttg?aag?gaa?gca?aaa?ccg 917
Asp?Val?Thr?Glu?Glu?Ser?Leu?Tyr?Arg?Gly?Leu?Lys?Glu?Ala?Lys?Pro
125 130 135
gga?gaa?cgt?ttg?tcg?aat?att?tcc?cac?gca?ata?caa?acg?tat?gtc?gaa 965
Gly?Glu?Arg?Leu?Ser?Asn?Ile?Ser?His?Ala?Ile?Gln?Thr?Tyr?Val?Glu
140 145 150 155
agc?gaa?caa?ttt?tca?gtc?gtc?agg?gaa?tat?gta?gga?cac?ggg?gta?ggg 1013
Ser?Glu?Gln?Phe?Ser?Val?Val?Arg?Glu?Tyr?Val?Gly?His?Gly?Val?Gly
160 165 170
cag?gat?ctg?cat?gag?gat?ccg?caa?att?cct?cac?tac?ggt?ccg?ccg?aac 1061
Gln?Asp?Leu?His?Glu?Asp?Pro?Gln?Ile?Pro?His?Tyr?Gly?Pro?Pro?Asn
175 180 185
aaa?ggc?ccg?cgt?tta?aag?cct?ggc?atg?gtc?ttg?gcc?att?gag?ccg?atg 1109
Lys?Gly?Pro?Arg?Leu?Lys?Pro?Gly?Met?Val?Leu?Ala?Ile?Glu?Pro?Met
190 195 200
gtc?aat?gca?ggt?aca?cgc?tat?gtc?aaa?aca?ttg?gct?gac?aac?tgg?acg 1157
Val?Asn?Ala?Gly?Thr?Arg?Tyr?Val?Lys?Thr?Leu?Ala?Asp?Asn?Trp?Thr
205 210 215
gtt?gta?act?gtg?gac?ggg?aag?aag?tgt?gct?cac?ttt?gaa?cac?acg?att 1205
Val?Val?Thr?Val?Asp?Gly?Lys?Lys?Cys?Ala?His?Phe?Glu?His?Thr?Ile
220 225 230 235
gcc?att?acg?gat?gcc?ggt?ttt?gag?ata?ctg?aca?aaa?gcc?taggtgaaat 1254
Ala?Ile?Thr?Asp?Ala?Gly?Phe?Glu?Ile?Leu?Thr?Lys?Ala
240 245
gagatgtatc?ggaaaacacc?tgaaattgga?cagttcgtac?gcatgacaag?aggccgcgaa 1314
atgaaccaat?acgcagttgt?tattgatata?gtcgatcatc?atcatgtgct?ggtagcagac 1374
ggagaaaagc?gtaaatttca?ttctcctaag?aaaaagaata?tcaatcattt?aaccttttat 1434
gattgcgtat?ctccggaagt?tcagaacagt?ataaacgaaa?cagggcgtgt?gacaaacgga 1494
aaattgagat?tttctctttt?gaaatttgtc?agagagcaag?ttactgattt?gaagaaggga 1554
gaacacttga?atggcgaaag?acgatgtaat?tgaagtggaa?ggtactgtag?tcgaaacgct 1614
gccaaacgca?atgttcaaag?ttgaacttga?gaatggccac?acggttttgg?ctcacgtatc 1674
tggtaaaatc?cgcatgcact?tcattcgcat?tttacctgga?gacaaagtta?cggtagaatt 1734
atctccatat?gac 1747
<210>34
<211>248
<212>PRT
＜213〉Bacillus licheniformis
<400>34
Met?Ile?Ile?Cys?Lys?Thr?Pro?Arg?Glu?Ile?Glu?Ile?Met?Arg?Glu?Ala
1 5 10 15
Gly?Arg?Ile?Val?Ala?Leu?Thr?His?Gln?Glu?Leu?Lys?Lys?His?Ile?Lys
20 25 30
Pro?Gly?Ile?Ser?Thr?Lys?Glu?Leu?Asp?Gln?Ile?Ala?Glu?Arg?Phe?Ile
35 40 45
Thr?Lys?Gln?Gly?Ala?Ile?Pro?Ser?Phe?Lys?Gly?Tyr?Asn?Gly?Phe?Arg
50 55 60
Gly?Ser?Ile?Cys?Val?Ser?Val?Asn?Glu?Glu?Leu?Val?His?Gly?Ile?Pro
65 70 75 80
Gly?Lys?Arg?Val?Leu?Arg?Asp?Gly?Asp?Ile?Ile?Ser?Ile?Asp?Ile?Gly
85 90 95
Ala?Lys?Leu?Asn?Gly?Tyr?His?Gly?Asp?Ser?Ala?Trp?Thr?Tyr?Pro?Val
100 105 110
Gly?Thr?Ile?Ser?Asp?Asp?Asp?Gln?Lys?Leu?Leu?Asp?Val?Thr?Glu?Glu
115 120 125
Ser?Leu?Tyr?Arg?Gly?Leu?Lys?Glu?Ala?Lys?Pro?Gly?Glu?Arg?Leu?Ser
130 135 140
Asn?Ile?Ser?His?Ala?Ile?Gln?Thr?Tyr?Val?Glu?Ser?Glu?Gln?Phe?Ser
145 150 155 160
Val?Val?Arg?Glu?Tyr?Val?Gly?His?Gly?Val?Gly?Gln?Asp?Leu?His?Glu
165 170 175
Asp?Pro?Gln?Ile?Pro?His?Tyr?Gly?Pro?Pro?Asn?Lys?Gly?Pro?Arg?Leu
180 185 190
Lys?Pro?Gly?Met?Val?Leu?Ala?Ile?Glu?Pro?Met?Val?Asn?Ala?Gly?Thr
195 200 205
Arg?Tyr?Val?Lys?Thr?Leu?Ala?Asp?Asn?Trp?Thr?Val?Val?Thr?Val?Asp
210 215 220
Gly?Lys?Lys?Cys?Ala?His?Phe?Glu?His?Thr?Ile?Ala?Ile?Thr?Asp?Ala
225 230 235 240
Gly?Phe?Glu?Ile?Leu?Thr?Lys?Ala
245
<210>35
<211>1928
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1427)
<400>35
atcaccggct?tgaaaccggc?cgaagatctc?gagctcaaaa?cgggagaaac?cgtgaaaatc 60
gagtttgaaa?gcgccgctga?tttggatgct?gtctttgtga?tcagaatgcc?gctgaccaat 120
ttcaaaaccg?ctgcccaaaa?cgtaacggag?ctgccgatca?gagaagtctc?aaaaggaaaa 180
tatgaaggat?attggaccgc?tacttcaact?gcaaaagcaa?aaggagcgga?aatcgaggtc 240
atcgtcagag?atgattacgg?caatgaaacg?agacaaacgg?caaaaggcaa?gctgtatatc 300
aatgaaaagc?tgaaataaag?gtgaaaagac?gctgtcttta?atggcagcgt?ttttttcgtt 360
ttacgatcga?caaattcagt?acgaaaactt?caaaaaatgt?acgatttacg?caacattaat 420
tgacagactt?tacctttggg?cttgatttat?acttaggaaa?acaaacacta?aggtcaccga 480
gccgcagaaa?ggggaaggat?gtg?gaa?atc?tat?tta?gat?gcg?ata?tgg?ctg?tta 533
Val?Glu?Ile?Tyr?Leu?Asp?Ala?Ile?Trp?Leu?Leu
1 5 10
aac?ttt?tgt?ttt?gac?ttg?ctg?ctt?tta?atg?atg?acc?gca?ttt?att?tta 581
Asn?Phe?Cys?Phe?Asp?Leu?Leu?Leu?Leu?Met?Met?Thr?Ala?Phe?Ile?Leu
15 20 25
aag?cga?agg?gtt?aaa?aag?cgg?agg?ctg?atc?cta?ggg?gca?ttt gtc?gcg 629
Lys?Arg?Arg?Val?Lys?Lys?Arg?Arg?Leu?Ile?Leu?Gly?Ala?Phe?Val?Ala
30 35 40
tca?agc?atc?gtt?ctg?ttt?atg?ttt?aca?cct?ttt?tca?ccg?tac?gtc?ctt 677
Ser?Ser?Ile?Val?Leu?Phe?Met?Phe?Thr?Pro?Phe?Ser?Pro?Tyr?Val?Leu
45 50 55
cat?cct?gcc?ggc?aaa?ctg?tcg?ttt?tcg?gtt?gtg?atc?gtt?ctt?gtg?gca 725
His?Pro?Ala?Gly?Lys?Leu?Ser?Phe?Ser?Val?Val?Ile?Val?Leu?Val?Ala
60 65 70 75
ttt?ggt?ttt?aag?cgg?ttc?cgg?ttt?ttt?ttg?cag?aat?ttg?ttt?tct?ttt 773
Phe?Gly?Phe?Lys?Arg?Phe?Arg?Phe?Phe?Leu?Gln?Asn?Leu?Phe?Ser?Phe
80 85 90
tat?ttt?gcc?act?ttt?tta?atg?gga?gga?ggg?att?atc?gga?gcg?cat?tct 821
Tyr?Phe?Ala?Thr?Phe?Leu?Met?Gly?Gly?Gly?Ile?Ile?Gly?Ala?His?Ser
95 100 105
ttg?ctt?gaa?acg?gat?tcg?atc?atg?gaa?aac?ggc?gtc?ttt?atg?acg?aat 869
Leu?Leu?Glu?Thr?Asp?Ser?Ile?Met?Glu?Asn?Gly?Val?Phe?Met?Thr?Asn
110 115 120
tgg?tcc?ggt?ttt?gga?gac?ccc?gtc?agc?tgg?ctg?ttt?gtc?tgt?gtg?ggt 917
Trp?Ser?Gly?Phe?Gly?Asp?Pro?Val?Ser?Trp?Leu?Phe?Val?Cys?Val?Gly
125 130 135
ttt?gcg?gct?gta?tgg?ctg?ttt?tca?aaa?aag?cgt?ttt?gaa?gat?gct?gaa 965
Phe?Ala?Ala?Val?Trp?Leu?Phe?Ser?Lys?Lys?Arg?Phe?Glu?Asp?Ala?Glu
140 145 150 155
gcg?aag?aaa?att?caa?tac?gaa?gaa?cgc?gtc?cgc?cta?gag?gcc?tgc?att 1013
Ala?Lys?Lys?Ile?Gln?Tyr?Glu?Glu?Arg?Val?Arg?Leu?Glu?Ala?Cys?Ile
160 165 170
ggt?gaa?cat?acg?ctt?cat?ttc?acc?gga?ttg?att?gac?tcc?gga?aac?cag 1061
Gly?Glu?His?Thr?Leu?His?Phe?Thr?Gly?Leu?Ile?Asp?Ser?Gly?Asn?Gln
175 180 185
ctc?tac?gat?cca?atc?aca?aaa?acg?ccc?gtc?atg?atc?gtc?aat?att?gaa 1109
Leu?Tyr?Asp?Pro?Ile?Thr?Lys?Thr?Pro?Val?Met?Ile?Val?Asn?Ile?Glu
190 195 200
aaa?ttg?aaa?gtt?gta?ttg?gga?gaa?gag?gca?agt?gtg?acc?atc?aag?gaa 1157
Lys?Leu?Lys?Val?Val?Leu?Gly?Glu?Glu?Ala?Ser?Val?Thr?Ile?Lys?Glu
205 210 215
atg?agc?ccg?ctt?gat?gcc?gtc?ggg?aaa?ctg?gat?gaa?gca?ctg?ccg?tat 1205
Met?Ser?Pro?Leu?Asp?Ala?Val?Gly?Lys?Leu?Asp?Glu?Ala?Leu?Pro?Tyr
220 225 230 235
atc?ggg?cgg?atc?cgc?ctg?att?ccg?tac?cgc?ggg?gtc?ggc?cat?cag?cat 1253
Ile?Gly?Arg?Ile?Arg?Leu?Ile?Pro?Tyr?Arg?Gly?Val?Gly?His?Gln?His
240 245 250
cag?ttt?ctg?ctc?tgc?tta?aag?ccg?gat?cat?gtg?ctc?gtt?tgt?acg?gaa 1301
Gln?Phe?Leu?Leu?Cys?Leu?Lys?Pro?Asp?His?Val?Leu?Val?Cys?Thr?Glu
255 260 265
aga?gaa?gtg?att?gaa?gcg?ccg?aaa?tgc?ctg?att?ggc?atc?agc?aca?tca 1349
Arg?Glu?Val?Ile?Glu?Ala?Pro?Lys?Cys?Leu?Ile?Gly?Ile?Ser?Thr?Ser
270 275 280
ccg?ctt?tcc?gct?gac?ggc?gaa?ttt?gac?gcc?atc?gtc?cat?ccg?aaa?atg 1397
Pro?Leu?Ser?Ala?Asp?Gly?Glu?Phe?Asp?Ala?Ile?Val?His?Pro?Lys?Met
285 290 295
ctg?gcc?gga?aac?ccg?gtc?aaa?cac?gtt?tct?taaacttgaa?gtctgttaca 1447
Leu?Ala?Gly?Asn?Pro?Val?Lys?His?Val?Ser
300 305
ttatcatact?cctgaagacg?tttatttaga?agggggagga?agatgaaaaa?actaaaatta 1507
aggttaacct?atctatggta?caaactttta?atgaaactgg?ggctgaaaag?cgacgaaatt 1567
tattatatcg?gcggaagcga?ggcgcttccc?ccgccattgt?caaaagatga?agagcaggtg 1627
cttctccata?agctgcctga?cggtgatcag?gcggcacgag?cgattttgat?tgaacgaaat 1687
ctcagactgg?tcgtgtacat?cgcgagaaaa?tttgaaaata?caggaatcaa?tatcgaggat 1747
ttaatctcca?tcggcacgat?cgggctcatc?aaagcggtga?atacgtttaa?tcccgagaaa 1807
aaaatcaaac?tggctacata?tgcttccaga?tgcattgaaa?atgaaatttt?gatgtattta 1867
agaagaaaca?ataaaatccg?ttcagaggta?tcattcgacg?aaccgctgaa?catcgattgg 1927
g 1928
<210>36
<211>309
<212>PRT
＜213〉Bacillus licheniformis
<400>36
Val?Glu?Ile?Tyr?Leu?Asp?Ala?Ile?Trp?Leu?Leu?Asn?Phe?Cys?Phe?Asp
1 5 10 15
Leu?Leu?Leu?Leu?Met?Met?Thr?Ala?Phe?Ile?Leu?Lys?Arg?Arg?Val?Lys
20 25 30
Lys?Arg?Arg?Leu?Ile?Leu?Gly?Ala?Phe?Val?Ala?Ser?Ser?Ile?Val?Leu
35 40 45
Phe?Met?Phe?Thr?Pro?Phe?Ser?Pro?Tyr?Val?Leu?His?Pro?Ala?Gly?Lys
50 55 60
Leu?Ser?Phe?Ser?Val?Val?Ile?Val?Leu?Val?Ala?Phe?Gly?Phe?Lys?Arg
65 70 75 80
Phe?Arg?Phe?Phe?Leu?Gln?Asn?Leu?Phe?Ser?Phe?Tyr?Phe?Ala?Thr?Phe
85 90 95
Leu?Met?Gly?Gly?Gly?Ile?Ile?Gly?Ala?His?Ser?Leu?Leu?Glu?Thr?Asp
100 105 110
Ser?Ile?Met?Glu?Asn?Gly?Val?Phe?Met?Thr?Asn?Trp?Ser?Gly?Phe?Gly
115 120 125
Asp?Pro?Val?Ser?Trp?Leu?Phe?Val?Cys?Val?Gly?Phe?Ala?Ala?Val?Trp
130 135 140
Leu?Phe?Ser?Lys?Lys?Arg?Phe?Glu?Asp?Ala?Glu?Ala?Lys?Lys?Ile?Gln
145 150 155 160
Tyr?Glu?Glu?Arg?Val?Arg?Leu?Glu?Ala?Cys?Ile?Gly?Glu?His?Thr?Leu
165 170 175
His?Phe?Thr?Gly?Leu?Ile?Asp?Ser?Gly?Asn?Gln?Leu?Tyr?Asp?Pro?Ile
180 185 190
Thr?Lys?Thr?Pro?Val?Met?Ile?Val?Asn?Ile?Glu?Lys?Leu?Lys?Val?Val
195 200 205
Leu?Gly?Glu?Glu?Ala?Ser?Val?Thr?Ile?Lys?Glu?Met?Ser?Pro?Leu?Asp
210 215 220
Ala?Val?Gly?Lys?Leu?Asp?Glu?Ala?Leu?Pro?Tyr?Ile?Gly?Arg?Ile?Arg
225 230 235 240
Leu?Ile?Pro?Tyr?Arg?Gly?Val?Gly?His?Gln?His?Gln?Phe?Leu?Leu?Cys
245 250 255
Leu?Lys?Pro?Asp?His?Val?Leu?Val?Cys?Thr?Glu?Arg?Glu?Val?Ile?Glu
260 265 270
Ala?Pro?Lys?Cys?Leu?Ile?Gly?Ile?Ser?Thr?Ser?Pro?Leu?Ser?Ala?Asp
275 280 285
Gly?Glu?Phe?Asp?Ala?Ile?Val?His?Pro?Lys?Met?Leu?Ala?Gly?Asn?Pro
290 295 300
Val?Lys?His?Val?Ser
305
<210>37
<211>1390
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1310)
<400>37
gaagaatttg?ctcaagccgc?cgtcagaaac?agcagcctca?cgaaagaaga?agcgtttcaa 60
tttctgattc?attcattgct?gggaacaggc?aagctgttaa?tggaaaaaga?catgacgttt 120
gctcaaacgc?ttgatcgggt?ggcgacaaaa?ggcggcataa?ccgcagaagg?tgcggaagtc 180
attcaagatg?ccatgccgga?agtctttgac?gagctgtttg?ccagaacgat?gaagaaatat 240
gaagtcatca?aagacgctgt?ccgccggcag?tgaaccgcgg?cattcatcga?catatccgga 300
tatccgcaag?ctgcctgacc?aggcagcttt?tttatttgca?ttcgacaaca?ttcatcaaaa 360
ttccaatttt?atattaaatt?tctaaaatta?ccaattgtta?ttgcatttct?attaaaattc 420
taatataatt?tgttagaata?ttcccaaaat?aataaaaaat?ttgagacgtt?tttaaggggt 480
ggttttttgt?gaagctgtat?atg?tct?gtt?gat?atg?gag?ggg?att?tcc?ggg?ctt 533
Met?Ser?Val?Asp?Met?Glu?Gly?Ile?Ser?Gly?Leu
1 5 10
ccg?gat?gat?acg?ttc?gtc?gat?tca?agc?aag?ccg?aac?tat?gag?aga?ggc 581
Pro?Asp?Asp?Thr?Phe?Val?Asp?Ser?Ser?Lys?Pro?Asn?Tyr?Glu?Arg?Gly
15 20 25
cgg?aag?atc?atg?acg?gac?gag?gcc?aat?cat?gtg?att?gac?gaa?gcg?ttc 629
Arg?Lys?Ile?Met?Thr?Asp?Glu?Ala?Asn?His?Val?Ile?Asp?Glu?Ala?Phe
30 35 40
aaa?aat?gga?tgc?aga?gag?gtg?att?gtt?aat?gac?agc?cac?tct?aaa?atg 677
Lys?Asn?Gly?Cys?Arg?Glu?Val?Ile?Val?Asn?Asp?Ser?His?Ser?Lys?Met
45 50 55
aac?aac?ctg?tta?att?gag?agg?ctc?cat?cct?gaa?gcg?cgg?ctt?att?tcg 725
Asn?Asn?Leu?Leu?Ile?Glu?Arg?Leu?His?Pro?G1u?Ala?Arg?Leu?Ile?Ser
60 65 70 75
gga?gat?gtc?aag?ccg?ttt?tcg?atg?gtt?cag?ggg?ctt?gat?gat?aca?tac 773
G1y?Asp?Val?Lys?Pro?Phe?Ser?Met?Val?Gln?G1y?Leu?Asp?Asp?Thr?Tyr
80 85 90
gac?gga?gcc?gtg?ttc?gtc?ggc?tac?cat?gcg?aga?gcg?tcg?atg?aga?ggc 821
Asp?Gly?Ala?Val?Phe?Val?Gly?Tyr?His?Ala?Arg?Ala?Ser?Met?Arg?Gly
95 100 105
gtg?atg?tcg?cac?agc?atg?att?ttc?ggc?gtc?aga?cat?ttt?tac?atc?aac 869
Val?Met?Ser?His?Ser?Met?Ile?Phe?Gly?Val?Arg?His?Phe?Tyr?Ile?Asn
110 115 120
gat?cag?gcg?gtc?ggc?gag?ctg?ggg?ttc?aac?gcc?tat?gtt?gcc?ggt?tat 917
Asp?Gln?Ala?Val?Gly?Glu?Leu?Gly?Phe?Asn?Ala?Tyr?Val?Ala?Gly?Tyr
125 130 135
tac?ggc?gtc?ccg?gtc?atc?atg?gct?gcc?ggt?gac?agc?gag?gcg?gca?gct 965
Tyr?Gly?Val?Pro?Val?Ile?Met?Ala?Ala?Gly?Asp?Ser?Glu?Ala?Ala?Ala
140 145 150 155
gag?gcg?gag?gag?ctg?att?ccg?aac?gta?acg?aca?gca?gcc?gtc?aaa?gaa 1013
Glu?Ala?Glu?Glu?Leu?Ile?Pro?Asn?Val?Thr?Thr?Ala?Ala?Val?Lys?Glu
160 165 170
acg?gtc?tcc?cgt?tcg?gcg?gtc?aaa?tgt?ctg?acg?ccg?gag?aaa?gcg?ggg 1061
Thr?Val?Ser?Arg?Ser?Ala?Val?Lys?Cys?Leu?Thr?Pro?Glu?Lys?Ala?Gly
175 180 185
cgg?cta?tta?aga?gaa?cgg?atg?gca?tat?gcg?att?caa?aac?cgc?ggt?caa 1109
Arg?Leu?Leu?Arg?Glu?Arg?Met?Ala?Tyr?Ala?Ile?Gln?Asn?Arg?Gly?Gln
190 195 200
gtc?aag?ccg?ctc?aca?cct?ccg?gac?agg?ccg?gtg?ctg?cgg?ata?gaa?ttt 1157
Val?Lys?Pro?Leu?Thr?Pro?Pro?Asp?Arg?Pro?Val?Leu?Arg?Ile?Glu?Phe
205 210 215
gce?aat?tac?ggc?caa?gcc?gag?tgg?gcc?agt?ctg?atg?ccg?ggt?gct?gaa 1205
Ala?Asn?Tyr?Gly?Gln?Ala?Glu?Trp?Ala?Ser?Leu?Met?Pro?Gly?Ala?Glu
220 225 230 235
atc?gaa?ccg?caa?acg?acg?act?gtc?cgc?tat?caa?gcg?aaa?gac?att?ctt 1253
Ile?Glu?Pro?Gln?Thr?Thr?Thr?Val?Arg?Tyr?Gln?Ala?Lys?Asp?Ile?Leu
240 245 250
gaa?gcc?tat?caa?gcg?atg?ctt?gtc?atg?acc?gag?ctt?gcg?atg?aga?acg 1301
Glu?Ala?Tyr?Gln?Ala?Met?Leu?Val?Met?Thr?Glu?Leu?Ala?Met?Arg?Thr
255 260 265
aca?ttc?tcc?taggaaggtg?ggatgaaaat?ggcgggttac?atcatgaagc 1350
Thr?Phe?Ser
270
ggctgctgtg?gatgctggca?accattatgg?tactccgagg 1390
<210>38
<211>270
<212>PRT
＜213〉Bacillus licheniformis
<400>38
Met?Ser?Val?Asp?Met?Glu?Gly?Ile?Ser?Gly?Leu?Pro?Asp?Asp?Thr?Phe
1 5 10 15
Val?Asp?Ser?Ser?Lys?Pro?Asn?Tyr?Glu?Arg?Gly?Arg?Lys?Ile?Met?Thr
20 25 30
Asp?Glu?Ala?Asn?His?Val?Ile?Asp?Glu?Ala?Phe?Lys?Asn?Gly?Cys?Arg
35 40 45
Glu?Val?Ile?Val?Asn?Asp?Ser?His?Ser?Lys?Met?Asn?Asn?Leu?Leu?Ile
50 55 60
Glu?Arg?Leu?His?Pro?Glu?Ala?Arg?Leu?Ile?Ser?Gly?Asp?Val?Lys?Pro
65 70 75 80
Phe?Ser?Met?Val?Gln?Gly?Leu?Asp?Asp?Thr?Tyr?Asp?Gly?Ala?Val?Phe
85 90 95
Val?Gly?Tyr?His?Ala?Arg?Ala?Ser?Met?Arg?Gly?Val?Met?Ser?His?Ser
100 105 110
Met?Ile?Phe?Gly?Val?Arg?His?Phe?Tyr?Ile?Asn?Asp?Gln?Ala?Val?Gly
115 120 125
Glu?Leu?Gly?Phe?Asn?Ala?Tyr?Val?Ala?Gly?Tyr?Tyr?Gly?Val?Pro?Val
130 135 140
Ile?Met?Ala?Ala?Gly?Asp?Ser?Glu?Ala?Ala?Ala?Glu?Ala?Glu?Glu?Leu
145 150 155 160
Ile?Pro?Asn?Val?Thr?Thr?Ala?Ala?Val?Lys?Glu?Thr?Val?Ser?Arg?Ser
165 170 175
Ala?Val?Lys?Cys?Leu?Thr?Pro?Glu?Lys?Ala?Gly?Arg?Leu?Leu?Arg?Glu
180 185 190
Arg?Met?Ala?Tyr?Ala?Ile?Gln?Asn?Arg?Gly?Gln?Val?Lys?Pro?Leu?Thr
195 200 205
Pro?Pro?Asp?Arg?Pro?Val?Leu?Arg?Ile?Glu?Phe?Ala?Asn?Tyr?Gly?Gln
210 215 220
Ala?Glu?Trp?Ala?Ser?Leu?Met?Pro?Gly?Ala?Glu?Ile?Glu?Pro?Gln?Thr
225 230 235 240
Thr?Thr?Val?Arg?Tyr?Gln?Ala?Lys?Asp?Ile?Leu?Glu?Ala?Tyr?Gln?Ala
245 250 255
Met?Leu?Val?Met?Thr?Glu?Leu?Ala?Met?Arg?Thr?Thr?Phe?Ser
260 265 270
<210>39
<211>2119
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(745)..(1974)
<400>39
gcttctttta?aagttaatcc?cagtgctgct?tttactaggc?gttcttcttc?gtcctcagtc 60
aagctaacga?ttgcccgatt?ttgcccttga?ttcatttcga?tcattttctt?gagcgtttct 120
ttaatttctg?caaagctggg?caattgaaac?tctacaattg?tgatgtcctt?ttccaattca 180
ggaggaagcg?ttaatgttgg?cgccacaagc?acaacatttt?tggaaacaag?actcttttcc 240
aatacagaga?caagatcttt?cattttacga?atcaaacggt?attcaggggg?gcgattattt 300
gcacccaaat?aaacataaaa?atccttcaat?ataaagactg?ctggctgatc?atatttttca 360
atgaacgcca?aagcctttaa?cgggtctttt?gtttcggctg?atggaggcat?tccattgctt 420
tcaaaccctt?tggttaaact?ccaaacaaat?aaggttcttg?gtgttctaat?caattctgta 480
tccgaaacaa?tatgactcag?ttgatgtatg?acacgttctt?cttcccatgt?agaaatatac 540
aggaacggaa?agcgggccct?gaaataattc?gctacttctt?ttctaaagtg?atgatatcct 600
tccatttttc?tccctttcta?ttgacctatt?ttttattatt?atactaaatt?tttccacaac 660
aggaataaaa?gatttcaatc?ctcccattcg?ccttctctaa?atgataagat?taaaataaaa 720
ccaacaattg?gagatgaagg?aaac?atg?aag?aac?aaa?ttg?att?gaa?cgc?ctc 771
Met?Lys?Asn?Lys?Leu?Ile?Glu?Arg?Leu
1 5
att?tct?tat?gcg?aaa?gtg?gat?acg?cag?tca?aat?gaa?aac?agt?cag?acg 819
Ile?Ser?Tyr?Ala?Lys?Val?Asp?Thr?Gln?Ser?Asn?Glu?Asn?Ser?Gln?Thr
10 15 20 25
acg?cct?tcc?act?ccc?ggg?cag?ctg?gcg?ctt?gcc?aat?atg?ctt?gtc?gaa 867
Thr?Pro?Ser?Thr?Pro?Gly?Gln?Leu?Ala?Leu?Ala?Asn?Met?Leu?Val?Glu
30 35 40
gag?ctg?aaa?gag?atc?ggc?atg?aag?gac?gtc?aca?atc?gat?gaa?aac?ggc 915
Glu?Leu?Lys?Glu?Ile?Gly?Met?Lys?Asp?Val?Thr?Ile?Asp?Glu?Asn?Gly
45 50 55
tat?gtg?atg?gcg?acg?ctt?cct?tcg?aat?aca?gaa?aaa?gag?gtg?ccg?acg 963
Tyr?Val?Met?Ala?Thr?Leu?Pro?Ser?Asn?Thr?Glu?Lys?Glu?Val?Pro?Thr
60 65 70
atc?ggt?ttc?ttg?gct?cat?gtg?gat?aca?gca?aca?gat?ttt?acc?gga?aag 1011
Ile?Gly?Phe?Leu?Ala?His?Val?Asp?Thr?Ala?Thr?Asp?Phe?Thr?Gly?Lys
75 80 85
aac?gtc?aat?ccg?cag?gtt?atc?gaa?caa?tac?gac?gga?aag?gat?att?gtg 1059
Asn?Val?Asn?Pro?Gln?Val?Ile?Glu?Gln?Tyr?Asp?Gly?Lys?Asp?Ile?Val
90 95 100 105
ctg?aat?gaa?tcc?ctc?aat?gtc?gtc?tta?tcg?ccc?aag?gaa?ttc?ccc?gag 1107
Leu?Asn?Glu?Ser?Leu?Asn?Val?Val?Leu?Ser?Pro?Lys?Glu?Phe?Pro?Glu
110 115 120
cta?gca?gat?tat?gcg?gga?cat?acg?ttg?att?aca?acc?gac?gga?acg?act 1155
Leu?Ala?Asp?Tyr?Ala?Gly?His?Thr?Leu?Ile?Thr?Thr?Asp?Gly?Thr?Thr
125 130 135
ttg?ctc?ggc?gcc?gac?aat?aaa?gct?ggg?atc?tct?gaa?atc?atg?acg?gca 1203
Leu?Leu?Gly?Ala?Asp?Asn?Lys?Ala?Gly?Ile?Ser?Glu?Ile?Met?Thr?Ala
140 145 150
atg?gaa?tat?tta?att?gcg?cac?ccc?gaa?atc?aaa?cac?ggg?aag?atc?aga 1251
Met?Glu?Tyr?Leu?Ile?Ala?His?Pro?Glu?Ile?Lys?His?Gly?Lys?Ile?Arg
155 160 165
gtc?gct?ttt?aca?cct?gat?gaa?gag?atc?ggc?aga?ggg?ccg?cac?aag?ttt 1299
Val?Ala?Phe?Thr?Pro?Asp?Glu?Glu?Ile?Gly?Arg?Gly?Pro?His?Lys?Phe
170 175 180 185
gac?gtc?gag?gcg?ttc?aat?gcc?aaa?ttc?gcg?tat?acg?gtt?gac?ggc?gga 1347
Asp?Val?Glu?Ala?Phe?Asn?Ala?Lys?Phe?Ala?Tyr?Thr?Val?Asp?Gly?Gly
190 195 200
ccg?ctt?ggc?gaa?ctg?cag?tac?gaa?agc?ttc?aat?gcc?gca?gcc?gcg?aaa 1395
Pro?Leu?Gly?Glu?Leu?Gln?Tyr?Glu?Ser?Phe?Asn?Ala?Ala?Ala?Ala?Lys
205 210 215
atc?act?tgt?aaa?gga?acg?aac?gtc?cac?ccc?ggc?aca?gca?aaa?gga?aaa 1443
Ile?Thr?Cys?Lys?Gly?Thr?Asn?Val?His?Pro?Gly?Thr?Ala?Lys?Gly?Lys
220 225 230
atg?gtc?aat?gcc?gcc?aaa?atc?gcg?atg?cag?ttc?cac?gcc?gca?ttg?ccg 1491
Met?Val?Asn?Ala?Ala?Lys?Ile?Ala?Met?Gln?Phe?His?Ala?Ala?Leu?Pro
235 240 245
gaa?aac?gaa?gct?cct?gaa?ttt?aca?gaa?ggt?tat?gaa?ggc?ttt?tat?cat 1539
Glu?Asn?Glu?Ala?Pro?Glu?Phe?Thr?Glu?Gly?Tyr?Glu?Gly?Phe?Tyr?His
250 255 260 265
ctg?ctt?tca?atc?aag?ggc?gat?gtt?tct?gaa?acg?agc?ctc?tct?tat?atc 1587
Leu?Leu?Ser?Ile?Lys?Gly?Asp?Val?Ser?Glu?Thr?Ser?Leu?Ser?Tyr?Ile
270 275 280
att?aga?gat?ttt?gac?aga?gac?cga?ttt?aac?gag?aga?aaa?gac?acc?gtc 1635
Ile?Arg?Asp?Phe?Asp?Arg?Asp?Arg?Phe?Asn?Glu?Arg?Lys?Asp?Thr?Val
285 290 295
caa?aaa?atc?gca?aac?aac?ctt?aaa?gcg?aaa?tac?ggc?gaa?aac?agc?gtc 1683
Gln?Lys?Ile?Ala?Asn?Asn?Leu?Lys?Ala?Lys?Tyr?Gly?Glu?Asn?Ser?Val
300 305 310
aca?gtg?gac?atg?aat?gat?caa?tat?tac?aac?atg?agg?gaa?aag?atc?gaa 1731
Thr?Val?Asp?Met?Asn?Asp?Gln?Tyr?Tyr?Asn?Met?Arg?Glu?Lys?Ile?Glu
315 320 325
ccg?gtc?aaa?gag?att?gtc?gac?att?gcc?tat?aaa?gcg?atg?aaa?aac?ctt 1779
Pro?Val?Lys?Glu?Ile?Val?Asp?Ile?Ala?Tyr?Lys?Ala?Met?Lys?Asn?Leu
330 335 340 345
gat?att?gaa?ccg?gtc?gtt?aaa?ccg?atc?cgc?ggc?ggt?aca?gac?ggc?tct 1827
Asp?Ile?Glu?Pro?Val?Val?Lys?Pro?Ile?Arg?Gly?Gly?Thr?Asp?Gly?Ser
350 355 360
cag?ctc?tca?tat?atg?ggg?ctt?cct?tgt?ccg?aac?att?ttc?aca?ggc?ggg 1875
Gln?Leu?Ser?Tyr?Met?Gly?Leu?Pro?Cys?Pro?Asn?Ile?Phe?Thr?Gly?Gly
365 370 375
gaa?aac?ttt?cac?gga?aaa?tac?gag?tat?att?tcc?gcc?gac?aat?atg?gta 1923
Glu?Asn?Phe?His?Gly?Lys?Tyr?Glu?Tyr?Ile?Ser?Ala?Asp?Asn?Met?Val
380 385 390
aaa?gcc?gcg?aat?gtc?atc?gtg?gaa?att?gtg?aag?ctg?ttt?gaa?gaa?aga 1971
Lys?Ala?Ala?Asn?Val?Ile?Val?Glu?Ile?Val?Lys?Leu?Phe?Glu?Glu?Arg
395 400 405
gct?taaacgaaaa?aagcggaaag?cggctgcaag?acaaagcagc?cgcttttttt 2024
Ala
410
tccagatcaa?tctttatctg?cgctttgctg?aaattagaac?atgttgcaag?gcggtttctt 2084
tttggagcgg?gataaagcca?attaacatct?caccg 2119
<210>40
<211>410
<212>PRT
＜213〉Bacillus licheniformis
<400>40
Met?Lys?Asn?Lys?Leu?Ile?Glu?Arg?Leu?Ile?Ser?Tyr?Ala?Lys?Val?Asp
1 5 10 15
Thr?Gln?Ser?Asn?Glu?Asn?Ser?Gln?Thr?Thr?Pro?Ser?Thr?Pro?Gly?Gln
20 25 30
Leu?Ala?Leu?Ala?Asn?Met?Leu?Val?Glu?Glu?Leu?Lys?Glu?Ile?Gly?Met
35 40 45
Lys?Asp?Val?Thr?Ile?Asp?Glu?Asn?Gly?Tyr?Val?Met?Ala?Thr?Leu?Pro
50 55 60
Ser?Asn?Thr?Glu?Lys?Glu?Val?Pro?Thr?Ile?Gly?Phe?Leu?Ala?His?Val
65 70 75 80
Asp?Thr?Ala?Thr?Asp?Phe?Thr?Gly?Lys?Asn?Val?Asn?Pro?Gln?Val?Ile
85 90 95
Glu?Gln?Tyr?Asp?Gly?Lys?Asp?Ile?Val?Leu?Asn?Glu?Ser?Leu?Asn?Val
100 105 110
Val?Leu?Ser?Pro?Lys?Glu?Phe?Pro?Glu?Leu?Ala?Asp?Tyr?Ala?Gly?His
115 120 125
Thr?Leu?Ile?Thr?Thr?Asp?Gly?Thr?Thr?Leu?Leu?Gly?Ala?Asp?Asn?Lys
130 135 140
Ala?Gly?Ile?Ser?Glu?Ile?Met?Thr?Ala?Met?Glu?Tyr?Leu?Ile?Ala?His
145 150 155 160
Pro?Glu?Ile?Lys?His?Gly?Lys?Ile?Arg?Val?Ala?Phe?Thr?Pro?Asp?Glu
165 170 175
Glu?Ile?Gly?Arg?Gly?Pro?His?Lys?Phe?Asp?Val?Glu?Ala?Phe?Asn?Ala
180 185 190
Lys?Phe?Ala?Tyr?Thr?Val?Asp?Gly?Gly?Pro?Leu?Gly?Glu?Leu?Gln?Tyr
195 200 205
Glu?Ser?Phe?Asn?Ala?Ala?Ala?Ala?Lys?Ile?Thr?Cys?Lys?Gly?Thr?Asn
210 215 220
Val?His?Pro?Gly?Thr?Ala?Lys?Gly?Lys?Met?Val?Asn?Ala?Ala?Lys?Ile
225 230 235 240
Ala?Met?Gln?Phe?His?Ala?Ala?Leu?Pro?Glu?Asn?Glu?Ala?Pro?Glu?Phe
245 250 255
Thr?Glu?Gly?Tyr?Glu?Gly?Phe?Tyr?His?Leu?Leu?Ser?Ile?Lys?Gly?Asp
260 265 270
Val?Ser?Glu?Thr?Ser?Leu?Ser?Tyr?Ile?Ile?Arg?Asp?Phe?Asp?Arg?Asp
275 280 285
Arg?Phe?Asn?Glu?Arg?Lys?Asp?Thr?Val?Gln?Lys?Ile?Ala?Asn?Asn?Leu
290 295 300
Lys?Ala?Lys?Tyr?Gly?Glu?Asn?Ser?Val?Thr?Val?Asp?Met?Asn?Asp?Gln
305 310 315 320
Tyr?Tyr?Asn?Met?Arg?Glu?Lys?Ile?Glu?Pro?Val?Lys?Glu?Ile?Val?Asp
325 330 335
Ile?Ala?Tyr?Lys?Ala?Met?Lys?Asn?Leu?Asp?Ile?Glu?Pro?Val?Val?Lys
340 345 350
Pro?Ile?Arg?Gly?Gly?Thr?Asp?Gly?Ser?Gln?Leu?Ser?Tyr?Met?Gly?Leu
355 360 365
Pro?Cys?Pro?Asn?Ile?Phe?Thr?Gly?Gly?Glu?Asn?Phe?His?Gly?Lys?Tyr
370 375 380
Glu?Tyr?Ile?Ser?Ala?Asp?Asn?Met?Val?Lys?Ala?Ala?Asn?Val?Ile?Val
385 390 395 400
Glu?Ile?Val?Lys?Leu?Phe?Glu?Glu?Arg?Ala
405 410
<210>41
<211>1782
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(1238)..(1780)
<400>41
acaggcacca?caacgcataa?tcacactcag?ccgagaccga?aaataaacac?ctcaccaaca 60
aacctaacac?caacacaccc?cacactcaaa?ccacccaagg?ataacaaaac?cgaacgacca 120
cgacatcacg?caaaaacggc?ccccacaagc?agacaaaata?caaccgcaca?tccaaaaaat 180
aaacacatcc?caaaataaag?acacacgaaa?agcaccacct?gacaacaaga?ccacacacac 240
aacaccgaga?acaacccacc?aaaaccggcc?ccacaaaaca?catcacacac?aaccgagacg 300
aaaaccgcag?caacgtccca?aaacaaccac?accccgccac?gatgacaaca?gacgcacaac 360
cgcgcataaa?gtagaaaaaa?agacgatacg?ccacagcaaa?aaacagacac?agcaccctta 420
acctcctacc?cccgcatgcc?atatacacac?tactcatccc?caccaaaaca?ccccagggca 480
cacgcccaca?ccgcaaccaa?tcccatcacg?gaagcgaaca?aaaaaaaatc?ggcgccatac 540
aacaccaacg?gaccggaaac?caagagaccc?gctgcgcgca?taatatatcg?ctgatacata 600
tttggaaaac?gttgaatgtt?ataacgaagt?acggggaatg?tacgggttta?ccggcactta 660
taaaggaaag?cgcgtttccg?ttcaggggac?gggcatgggt?gtgccgtcta?tttcaattta 720
tgtcaatgaa?ctgatcagaa?gctatgatgt?gaaaaacctg?atccgcgtcg?gatcgtgcgg 780
tgcgatcaga?aaagacgtca?atgtaagaga?tgtcattctt?gcgatgactt?catcaactga 840
ttcacaaatc?aacagagtgg?cattcggctc?gatcgacttt?gcgccctgcg?cggacttcgg 900
cctgctaaaa?gctgcttatg?acgctgcaag?tgagcggaac?atcccggtta?cggcagggaa 960
tgtgtttacg?gcggatcaat?tctataacga?cgacagccaa?attgagaagc?ttgccaaaca 1020
cggagtgctt?gcggtcgaga?tggaaacgac?ggcgctttat?acattggctg?cgaaattcgg 1080
ccgaaaagcc?ctctccattt?taacggtcag?cgatcacgtc?attaccggcg?aggaaacaac 1140
ggcagaagaa?agacagacga?cctttaatga?tatgattctg?ctggctctgg?atactgcttt 1200
gtaaaagcat?acatgcactg?aaaaaggatg?gattcga?atg?aac?gga?aag?tac?aaa 1255
Met?Asn?Gly?Lys?Tyr?Lys
1 5
tac?gtg?acg?att?gct?tca?ttg?ctg?agt?gca?gcg?gtc?ctg?ctt?ggc?aca 1303
Tyr?Val?Thr?Ile?Ala?Ser?Leu?Leu?Ser?Ala?Ala?Val?Leu?Leu?Gly?Thr
10 15 20
gga?tgt?acc?atg?acc?gaa?cag?aag?caa?aat?gac?acg?gct?aaa?aca?cag 1351
Gly?Cys?Thr?Met?Thr?Glu?Gln?Lys?Gln?Asn?Asp?Thr?Ala?Lys?Thr?Gln
25 30 35
aat?act?gca?aat?gca?agc?aag?gat?gca?aaa?gac?caa?aag?tca?gac?agc 1399
Asn?Thr?Ala?Asn?Ala?Ser?Lys?Asp?Ala?Lys?Asp?Gln?Lys?Ser?Asp?Ser
40 45 50
agc?ctg?gaa?gat?cag?gac?ttc?ata?ctc?gag?agc?aag?tac?ttt?aat?caa 1447
Ser?Leu?Glu?Asp?Gln?Asp?Phe?Ile?Leu?Glu?Ser?Lys?Tyr?Phe?Asn?Gln
55 60 65 70
ata?aaa?gat?gta?gac?ggg?ctg?ccg?acg?att?caa?aat?ccg?gaa?aac?ata 1495
Ile?Lys?Asp?Val?Asp?Gly?Leu?Pro?Thr?Ile?Gln?Asn?Pro?Glu?Asn?Ile
75 80 85
atg?gcg?ctt?gta?aat?aaa?gaa?tac?gcg?ctt?ccc?ggc?aac?tat?gca?ccg 1543
Met?Ala?Leu?Val?Asn?Lys?Glu?Tyr?Ala?Leu?Pro?Gly?Asn?Tyr?Ala?Pro
90 95 100
agc?gac?ctg?aca?gtg?cct?gat?gtc?gcg?ttt?tct?ttt?acc?gag?gat?atc 1591
Ser?Asp?Leu?Thr?Val?Pro?Asp?Val?Ala?Phe?Ser?Phe?Thr?Glu?Asp?Ile
105 110 115
gac?aag?cgt?tat?atc?aga?aaa?gaa?gcc?gcc?aaa?gcg?ctt?gaa?gag?atg 1639
Asp?Lys?Arg?Tyr?Ile?Arg?Lys?Glu?Ala?Ala?Lys?Ala?Leu?Glu?Glu?Met
120 125 130
ttc?agc?gcc?gct?aag?aaa?gaa?ggc?tat?gaa?ctc?gtt?gca?gtg?tcc?ggc 1687
Phe?Ser?Ala?Ala?Lys?Lys?Glu?Gly?Tyr?Glu?Leu?Val?Ala?Val?Ser?Gly
135 140 145 150
tac?aga?tcc?tat?gac?aga?caa?aaa?gcg?atc?tat?aac?aac?gaa?gtc?agc 1735
Tyr?Arg?Ser?Tyr?Asp?Arg?Gln?Lys?Ala?Ile?Tyr?Asn?Asn?Glu?Val?Ser
155 160 165
caa?aag?gga?gaa?gaa?aag?gcg?aaa?gaa?gct?gtg?gca?tat?ccg?ggc?ca 1782
Gln?Lys?Gly?Glu?Glu?Lys?Ala?Lys?Glu?Ala?Val?Ala?Tyr?Pro?Gly
170 175 180
<210>42
<211>181
<212>PRT
＜213〉Bacillus licheniformis
<400>42
Met?Asn?Gly?Lys?Tyr?Lys?Tyr?Val?Thr?Ile?Ala?Ser?Leu?Leu?Ser?Ala
1 5 10 15
Ala?Val?Leu?Leu?Gly?Thr?Gly?Cys?Thr?Met?Thr?Glu?Gln?Lys?Gln?Asn
20 25 30
Asp?Thr?Ala?Lys?Thr?Gln?Asn?Thr?Ala?Asn?Ala?Ser?Lys?Asp?Ala?Lys
35 40 45
Asp?Gln?Lys?Ser?Asp?Ser?Ser?Leu?Glu?Asp?Gln?Asp?Phe?Ile?Leu?Glu
50 55 60
Ser?Lys?Tyr?Phe?Asn?Gln?Ile?Lys?Asp?Val?Asp?Gly?Leu?Pro?Thr?Ile
65 70 75 80
Gln?Asn?Pro?Glu?Asn?Ile?Met?Ala?Leu?VaI?Asn?Lys?Glu?Tyr?Ala?Leu
85 90 95
Pro?Gly?Asn?Tyr?Ala?Pro?Ser?Asp?Leu?Thr?Val?Pro?Asp?Val?Ala?Phe
100 105 110
Ser?Phe?Thr?Glu?Asp?Ile?Asp?Lys?Arg?Tyr?Ile?Arg?Lys?Glu?Ala?Ala
115 120 125
Lys?Ala?Leu?Glu?Glu?Met?Phe?Ser?Ala?Ala?Lys?Lys?Glu?Gly?Tyr?Glu
130 135 140
Leu?Val?Ala?Val?Ser?Gly?Tyr?Arg?Ser?Tyr?Asp?Arg?Gln?Lys?Ala?Ile
145 150 155 160
Tyr?Asn?Asn?Glu?Val?Ser?Gln?Lys?Gly?Glu?Glu?Lys?Ala?Lys?Glu?Ala
165 170 175
Val?Ala?Tyr?Pro?Gly
180
<210>43
<211>1663
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(3)..(1166)
<400>43
tc?cgg?tct?gtg?gcc?atc?gga?gtt?tgg?att?gga?acc?gga?tca?aga?cat 47
Arg?Ser?Val?Ala?Ile?Gly?Val?Trp?Ile?Gly?Thr?Gly?Ser?Arg?His
1 5 10 15
gaa?aca?ccc?gaa?att?aac?ggc?att?tcg?cac?ttt?ctt?gag?cac?atg?ttt 95
Glu?Thr?Pro?Glu?Ile?Asn?Gly?Ile?Ser?His?Phe?Leu?Glu?His?Met?Phe
20 25 30
ttc?aag?ggg?aca?aaa?acg?cgc?act?gca?agg?gac?atc?gca?gag?tct?ttc 143
Phe?Lys?Gly?Thr?Lys?Thr?Arg?Thr?Ala?Arg?Asp?Ile?Ala?Glu?Ser?Phe
35 40 45
gat?aga?atc?ggc?gga?cag?gtc?aac?gcg?ttt?acc?tca?aag?gaa?tat?acg 191
Asp?Arg?Ile?Gly?Gly?Gln?Val?Asn?Ala?Phe?Thr?Ser?Lys?Glu?Tyr?Thr
50 55 60
tgc?tat?tac?gcc?aaa?gtg?ctc?gat?gag?cat?gca?agc?tat?gct?ctg?gaa 239
Cys?Tyr?Tyr?Ala?Lys?Val?Leu?Asp?Glu?His?Ala?Ser?Tyr?Ala?Leu?Glu
65 70 75
gtg?ctt?tct?gac?atg?ttc?ttt?cat?tca?tca?ttt?gac?gaa?gaa?gaa?tta 287
Val?Leu?Set?Asp?Met?Phe?Phe?His?Ser?Ser?Phe?Asp?Glu?Glu?Glu?Leu
80 85 90 95
aaa?aaa?gaa?aaa?aac?gtc?gtc?tat?gaa?gag?att?aaa?atg?tat?gag?gac 335
Lys?Lys?Glu?Lys?Asn?Val?Val?Tyr?Glu?Glu?Ile?Lys?Met?Tyr?Glu?Asp
100 105 110
aca?cct?gat?gac?atc?gtc?cac?gat?ctc?ttg?agc?aag?gcg?tca?tac?ggc 383
Thr?Pro?Asp?Asp?Ile?Val?His?Asp?Leu?Leu?Ser?Lys?Ala?Ser?Tyr?Gly
115 120 125
agc?cat?tcc?ctc?ggc?tat?ccg?atc?ctc?ggc?aca?gag?gaa?aca?ctt?gcc 431
Ser?His?Ser?Leu?Gly?Tyr?Pro?Ile?Leu?Gly?Thr?Glu?Glu?Thr?Leu?Ala
130 135 140
gaa?ttt?gac?gga?gat?tcg?ctc?aga?aaa?tat?atg?aac?gaa?tat?tac?acg 479
Glu?Phe?Asp?Gly?Asp?Ser?Leu?Arg?Lys?Tyr?Met?Asn?Glu?Tyr?Tyr?Thr
145 150 155
cct?gac?cga?gtc?gtc?att?tcc?ata?gcc?gga?aat?gtg?ccg?gaa?act?ttt 527
Pro?Asp?Arg?Val?Val?Ile?Ser?Ile?Ala?Gly?Asn?Val?Pro?Glu?Thr?Phe
160 165 170 175
ata?aaa?gaa?gct?gaa?aag?cat?ttc?ggt?tca?tat?gaa?gcc?aaa?ggc?aaa 575
Ile?Lys?Glu?Ala?Glu?Lys?His?Phe?Gly?Ser?Tyr?Glu?Ala?Lys?Gly?Lys
180 185 190
aga?acc?ggc?atg?aca?aaa?ccg?gat?ttt?cac?cat?gag?aaa?atg?acg?cgc 623
Arg?Thr?Gly?Met?Thr?Lys?Pro?Asp?Phe?His?His?Glu?Lys?Met?Thr?Arg
195 200 205
aaa?aag?gaa?acc?gaa?cag?gct?cac?ctc?tgc?ctc?ggc?ttc?aac?ggg?ctg 671
Lys?Lys?Glu?Thr?Glu?Gln?Ala?His?Leu?Cys?Leu?Gly?Phe?Asn?Gly?Leu
210 215 220
gaa?gca?ggc?cat?ccg?gaa?atc?tac?gat?ttg?atc?gtt?ctc?aat?aat?att 719
Glu?Ala?Gly?His?Pro?Glu?Ile?Tyr?Asp?Leu?Ile?Val?Leu?Asn?Asn?Ile
225 230 235
ttg?gga?gga?agc?atg?agc?agc?cgc?ctc?ttt?cag?gat?gtc?cgc?gaa?gat 767
Leu?Gly?Gly?Ser?Met?Ser?Ser?Arg?Leu?Phe?Gln?Asp?Val?Arg?Glu?Asp
240 245 250 255
aaa?ggt?ctt?gca?tat?tcc?gtg?ttc?agc?tac?cac?act?tcc?tat?gaa?gac 815
Lys?Gly?Leu?Ala?Tyr?Ser?Val?Phe?Ser?Tyr?His?Thr?Ser?Tyr?Glu?Asp
260 265 270
agc?ggc?atg?atg?acc?atc?tac?gcg?gga?acc?ggc?gca?aat?cag?ctt?cag 863
Ser?Gly?Met?Met?Thr?Ile?Tyr?Ala?Gly?Thr?Gly?Ala?Asn?Gln?Leu?Gln
275 280 285
ctc?ctg?tca?gaa?acg?att?cat?gaa?aca?ctg?cgt?gcg?ctg?aaa?agc?gac 911
Leu?Leu?Ser?Glu?Thr?Ile?His?Glu?Thr?Leu?Arg?Ala?Leu?Lys?Ser?Asp
290 295 300
ggt?att?aca?ccg?aaa?gag?ctt?gaa?aac?agc?aaa?gag?caa?atg?aaa?ggc 959
Gly?Ile?Thr?Pro?Lys?Glu?Leu?Glu?Asn?Ser?Lys?Glu?Gln?Met?Lys?Gly
305 310 315
agc?ctg?atg?ctc?agt?ctc?gaa?agc?aca?aac?agc?aaa?atg?agc?cgc?aac 1007
Ser?Leu?Met?Leu?Ser?Leu?Glu?Ser?Thr?Asn?Ser?Lys?Met?Ser?Arg?Asn
320 325 330 335
ggc?aaa?aat?gag?ctt?ttg?ctc?ggt?aaa?cac?cgg?acg?ctt?gat?gaa?atc 1055
Gly?Lys?Asn?Glu?Leu?Leu?Leu?Gly?Lys?His?Arg?Thr?Leu?Asp?Glu?Ile
340 345 350
atc?gaa?aag?ttg?aat?gcc?gtc?agc?ctc?gag?cgt?gtg?aac?aat?ctt?gcc 1103
Ile?Glu?Lys?Leu?Asn?Ala?Val?Ser?Leu?Glu?Arg?Val?Asn?Asn?Leu?Ala
355 360 365
aac?cgg?att?ttc?acc?gat?gat?tac?tca?tct?gca?tta?atc?agc?ccg?tcg 1151
Asn?Arg?Ile?Phe?Thr?Asp?Asp?Tyr?Ser?Ser?Ala?Leu?Ile?Ser?Pro?Ser
370 375 380
ggt?gag?ctg?ccg?aaa?taatgcctcc?ttaaaacatg?cttgtctttt?taggcaggca 1206
Gly?Glu?Leu?Pro?Lys
385
tgttttttgt?tatcggttca?tacatcataa?caaaaggggg?tttttttcat?gaggctgagt 1266
gaactttcgg?gaaaagaaat?cgttgatgtt?aagcgggccg?aacggctcgg?cgtactgggg 1326
cagacggacc?ttgagatcaa?tgagcaggac?ggtcagatta?ccgctcttat?catcccttct 1386
gttaaatggt?tcggactagg?aagaaaacag?gggaatgaca?ttaaggtgcc?ctggtcgcaa 1446
attcaaaaaa?tcggctcgga?tatgatcatt?ttggatgttc?cggaaagcag?cgtgacaaaa 1506
gaggagtaag?caaggtggaa?acccgcccgt?cccatggggg?cgggcttttt?gtttttctgg 1566
atttcaattc?accgctattt?cctttttgtc?atatgatgaa?attagcttat?gaattagatc 1626
cttgtcaaaa?aagaaggtga?atgttagagc?catgtta 1663
<210>44
<211>388
<212>PRT
＜213〉Bacillus licheniformis
<400>44
Arg?Ser?Val?Ala?Ile?Gly?Val?Trp?Ile?Gly?Thr?Gly?Ser?Arg?His?Glu
1 5 10 15
Thr?Pro?Glu?Ile?Asn?Gly?Ile?Ser?His?Phe?Leu?Glu?His?Met?Phe?Phe
20 25 30
Lys?Gly?Thr?Lys?Thr?Arg?Thr?Ala?Arg?Asp?Ile?Ala?Glu?Ser?Phe?Asp
35 40 45
Arg?Ile?Gly?Gly?Gln?Val?Asn?Ala?Phe?Thr?Ser?Lys?Glu?Tyr?Thr?Cys
50 55 60
Tyr?Tyr?Ala?Lys?Val?Leu?Asp?Glu?Hi Ala?Ser?Tyr?Ala?Leu?Glu?Val
65 70 75 80
Leu?Ser?Asp?Met?Phe?Phe?His?Ser?Ser?Phe?Asp?Glu?Glu?Glu?Leu?Lys
85 90 95
Lys?Glu?Lys?Asn?Val?Val?Tyr?Glu?Glu?Ile?Lys?Met?Tyr?Glu?Asp?Thr
100 105 110
Pro?Asp?Asp?Ile?Val?His?Asp?Leu?Leu?Ser?Lys?Ala?Ser?Tyr?Gly?Ser
115 120 125
His?Ser?Leu?Gly?Tyr?Pro?Ile?Leu?Gly?Thr?Glu?Glu?Thr?Leu?Ala?Glu
130 135 140
Phe?Asp?Gly?Asp?Ser?Leu?Arg?Lys?Tyr?Met?Asn?Glu?Tyr?Tyr?Thr?Pro
145 150 155 160
Asp?Arg?Val?Val?Ile?Ser?Ile?Ala?Gly?Asn?Val?Pro?Glu?Thr?Phe?Ile
165 170 175
Lys?Glu?Ala?Glu?Lys?His?Phe?Gly?Ser?Tyr?Glu?Ala?Lys?Gly?Lys?Arg
180 185 190
Thr?Gly?Met?Thr?Lys?Pro?Asp?Phe?His?His?Glu?Lys?Met?Thr?Arg?Lys
195 200 205
Lys?Glu?Thr?Glu?Gln?Ala?His?Leu?Cys?Leu?Gly?Phe?Asn?Gly?Leu?Glu
210 215 220
Ala?Gly?His?Pro?Glu?Ile?Tyr?Asp?Leu?Ile?Val?Leu?Asn?Asn?Ile?Leu
225 230 235 240
Gly?Gly?Ser?Met?Ser?Ser?Arg?Leu?Phe?Gln?Asp?Val?Arg?Glu?Asp?Lys
245 250 255
Gly?Leu?Ala?Tyr?Ser?Val?Phe?Ser?Tyr?His?Thr?Ser?Tyr?Glu?Asp?Ser
260 265 270
Gly?Met?Met?Thr?Ile?Tyr?Ala?Gly?Thr?Gly?Ala?Asn?Gln?Leu?Gln?Leu
275 280 285
Leu?Ser?Glu?Thr?Ile?His?Glu?Thr?Leu?Arg?Ala?Leu?Lys?Ser?Asp?Gly
290 295 300
Ile?Thr?Pro?Lys?Glu?Leu?Glu?Asn?Ser?Lys?Glu?Gln?Met?Lys?Gly?Ser
305 310 315 320
Leu?Met?Leu?Ser?Leu?Glu?Ser?Thr?Asn?Ser?Lys?Met?Ser?Arg?Asn?Gly
325 330 335
Lys?Asn?Glu?Leu?Leu?Leu?Gly?Lys?His?Arg?Thr?Leu?Asp?Glu?Ile?Ile
340 345 350
Glu?Lys?Leu?Asn?Ala?Val?Ser?Leu?Glu?Arg?Val?Asn?Asn?Leu?Ala?Asn
355 360 365
Arg?Ile?Phe?Thr?Asp?Asp?Tyr?Ser?Ser?Ala?Leu?Ile?Ser?Pro?Ser?Gly
370 375 380
Glu?Leu?Pro?Lys
385
<210>45
<211>1553
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(499)..(1296)
<400>45
ctttcaaatc?atcccagctg?aaatgccccg?aatcgttcct?ggagacgagg?aaggttccgt 60
ccgtctgggt?aagctgtgca?aaattaatca?ccggatcgct?tgcgccctgg?gcatttacgt 120
atattgacgt?ttccgagccg?acaagcgcaa?tatcggcgcc?gtttgacaga?agggcagtca 180
tcgttttatc?tccgccccag?gtcgttttca?gttcaacatc?aaggccttct?tctttaaaaa 240
aaccttcaga?taaggcgata?tataaaggcg?tatagaaaac?ggaacgggtc?acttccgcca 300
gacggatctt?ttcggtcttt?tgtccgccgc?atgaagcgag?gataaacagc?atcatgatga 360
aggataatat?ggtaaaaaag?aaacgtttca?aaccgatttc?cctcctttga?tttcggttat 420
tttcggcata?aaatagttta?tgttatatac?taaacaggtg?tgagggacag?ctgcggatta 480
aaagaggtga?cgtgacga?atg?tgg?ata?gaa?aaa?aga?agg?ttt?cca?tcg?ccg 531
Met?Trp?Ile?Glu?Lys?Arg?Arg?Phe?Pro?Ser?Pro
1 5 10
aac?aaa?gag?gtg?cgt?ctg?ttt?tcc?gtc?aca?tac?ctt?tca?caa?gga?ctc 579
Asn?Lys?Glu?Val?Arg?Leu?Phe?Ser?Val?Thr?Tyr?Leu?Ser?Gln?Gly?Leu
15 20 25
aag?gtt?aag?ggg?ctt?ttg?gcc?gaa?ccg?gcc?gct?ccc?gga?aat?tat?gac 627
Lys?Val?Lys?Gly?Leu?Leu?Ala?Glu?Pro?Ala?Ala?Pro?Gly?Asn?Tyr?Asp
30 35 40
ggg?ttt?ttg?tat?ttg?cgc?ggc?gga?att?aaa?aac?gtc?ggg?atg?gtc?aga 675
Gly?Phe?Leu?Tyr?Leu?Arg?Gly?Gly?Ile?Lys?Asn?Val?Gly?Met?Val?Arg
45 50 55
ccg?ggc?cga?atc?gtc?cag?ttc?gct?tca?cag?ggc?ttt?gtc?gtt?ctt?gct 723
Pro?Gly?Arg?Ile?Val?Gln?Phe?Ala?Ser?Gln?Gly?Phe?Val?Val?Leu?Ala
60 65 70 75
ccg?ttt?tac?agg?ggc?aat?caa?gga?gga?gaa?ggc?gat?gag?gat?ttt?gca 771
Pro?Phe?Tyr?Arg?Gly?Asn?Gln?Gly?Gly?Glu?Gly?Asp?Glu?Asp?Phe?Ala
80 85 90
ggt?aat?gac?cgg?gaa?gat?gct?ttt?gcc?gct?ttt?cgt?ctt?ctt?cag?aag 819
Gly?Asn?Asp?Arg?Glu?Asp?Ala?Phe?Ala?Ala?Phe?Arg?Leu?Leu?Gln?Lys
95 100 105
cac?gaa?ctg?gta?aag?gac?gga?cgc?gtt?cac?att?ttt?ggc?ttt?tcc?cgc 867
His?Glu?Leu?Val?Lys?Asp?Gly?Arg?Val?His?Ile?Phe?Gly?Phe?Ser?Arg
110 115 120
ggc?gga?atc?atg?ggc?atc?tta?acg?gca?atc?gaa?atg?aaa?gca?gcg?gcc 915
Gly?Gly?Ile?Met?Gly?Ile?Leu?Thr?Ala?Ile?Glu?Met?Lys?Ala?Ala?Ala
125 130 135
gct?tcg?ttt?gtg?tct?tgg?ggc?ggt?gtc?agc?gac?atg?gtc?atg?acc?tat 963
Ala?Ser?Phe?Val?Ser?Trp?Gly?Gly?Val?Ser?Asp?Met?Val?Met?Thr?Tyr
140 145 150 155
cgc?gaa?cga?aaa?gat?ttg?cgg?cgg?atg?atg?aag?cgg?gtc?atc?ggc?ggg 1011
Arg?Glu?Arg?Lys?Asp?Leu?Arg?Arg?Met?Met?Lys?Arg?Val?Ile?Gly?Gly
160 165 170
acg?cct?gaa?aag?gtt?ccc?gaa?gcg?tat?gaa?tgg?agg?acg?cct?ttt?gac 1059
Thr?Pro?Glu?Lys?Val?Pro?Glu?Ala?Tyr?Glu?Trp?Arg?Thr?Pro?Phe?Asp
175 180 185
gag?ctg?gag?aag?att?gaa?gcg?ccc?ggc?ttt?ttg?atc?cac?gga?gca?acc 1107
Glu?Leu?Glu?Lys?Ile?Glu?Ala?Pro?Gly?Phe?Leu?Ile?His?Gly?Ala?Thr
190 195 200
gtt?gaa?aat?gtc?tcc?atc?gag?cat?gcg?cgg?cgc?ctt?gaa?gcg?gga?ctt 1155
Val?Glu?Asn?Val?Ser?Ile?Glu?His?Ala?Arg?Arg?Leu?Glu?Ala?Gly?Leu
205 210 215
ctg?cga?ggg?aca?agt?ctg?ttg?gaa?act?ggg?aat?ttt?agc?cca?tat?acc 1203
Leu?Arg?Gly?Thr?Ser?Leu?Leu?Glu?Thr?Gly?Asn?Phe?Ser?Pro?Tyr?Thr
220 225 230 235
cac?tat?ttt?cct?ccg?cgc?gaa?aac?agg?cgg?att?gtt?aga?gaa?tta?tcc 1251
His?Tyr?Phe?Pro?Pro?Arg?Glu?Asn?Arg?Arg?Ile?Val?Arg?Glu?Leu?Ser
240 245 250
caa?tgg?atg?aaa?agc?cgt?cct?tgg?aca?agt?tat?ggt?aaa?cta?aag 1296
Gln?Trp?Met?Lys?Ser?Arg?Pro?Trp?Thr?Ser?Tyr?Gly?Lys?Leu?Lys
255 260 265
taaaagagaa?aaggaggggg?actttaggtg?ggaatgccag?ttgaatttaa?cacgatgatt 1356
gtgacaaaag?gaaaagaaac?gcgggttgag?gaaaatgtgt?ttacactcga?gaaagaaggt 1416
tatcgggttt?accctcttca?tgtgccgctt?gaagtcagaa?agaccaagcg?gggcgacatt 1476
accggcaccg?cgcaggcgga?gcgccttgaa?tggtcagacg?gacggactca?tttaacatac 1536
aggctcgtat?cattaaa 1553
<210>46
<211>266
<212>PRT
＜213〉Bacillus licheniformis
<400>46
Met?Trp?Ile?Glu?Lys?Arg?Arg?Phe?Pro?Ser?Pro?Asn?Lys?Glu?Val?Arg
1 5 10 15
Leu?Phe?Ser?Val?Thr?Tyr?Leu?Ser?Gln?Gly?Leu?Lys?Val?Lys?Gly?Leu
20 25 30
Leu?Ala?Glu?Pro?Ala?Ala?Pro?Gly?Asn?Tyr?Asp?Gly?Phe?Leu?Tyr?Leu
35 40 45
Arg?Gly?Gly?Ile?Lys?Asn?Val?Gly?Met?Val?Arg?Pro?Gly?Arg?Ile?Val
50 55 60
Gln?Phe?Ala?Ser?Gln?Gly?Phe?Val?Val?Leu?Ala?Pro?Phe?Tyr?Arg?Gly
65 70 75 80
Asn?Gln?Gly?Gly?Glu?Gly?Asp?Glu?Asp?Phe?Ala?Gly?Asn?Asp?Arg?Glu
85 90 95
Asp?Ala?Phe?Ala?Ala?Phe?Arg?Leu?Leu?Gln?Lys?His?Glu?Leu?Val?Lys
100 105 110
Asp?Gly?Arg?Val?His?Ile?Phe?Gly?Phe?Ser?Arg?Gly?Gly?Ile?Met?Gly
115 120 125
Ile?Leu?Thr?Ala?Ile?Glu?Met?Lys?Ala?Ala?Ala?Ala?Ser?Phe?Val?Ser
130 135 140
Trp?Gly?Gly?Val?Ser?Asp?Met?Val?Met?Thr?Tyr?Arg?Glu?Arg?Lys?Asp
145 150 155 160
Leu?Arg?Arg?Met?Met?Lys?Arg?Val?Ile?Gly?Gly?Thr?Pro?Glu?Lys?Val
165 170 175
Pro?Glu?Ala?Tyr?Glu?Trp?Arg?Thr?Pro?Phe?Asp?Glu?Leu?Glu?Lys?Ile
180 185 190
Glu?Ala?Pro?Gly?Phe?Leu?Ile?His?Gly?Ala?Thr?Val?Glu?Asn?Val?Ser
195 200 205
Ile?Glu?His?Ala?Arg?Arg?Leu?Glu?Ala?Gly?Leu?Leu?Arg?Gly?Thr?Ser
210 215 220
Leu?Leu?Glu?Thr?Gly?Asn?Phe?Ser?Pro?Tyr?Thr?His?Tyr?Phe?Pro?Pro
225 230 235 240
Arg?Glu?Asn?Arg?Arg?Ile?Val?Arg?Glu?Leu?Ser?Gln?Trp?Met?Lys?Ser
245 250 255
Arg?Pro?Trp?Thr?Ser?Tyr?Gly?Lys?Leu?Lys
260 265
<210>47
<211>1576
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1073)
<400>47
acgaagacga?aaggaacggt?ctgtgcaccg?ctgacattgt?ttgcagaata?taaaaaccgc 60
ggcagtcaaa?tcgccgactc?tgaatggaag?tgggagcttc?ataaactcgg?tcattccaaa 120
aagccgctag?aggacgggac?tgtgatcgat?aaaggcgtgt?tcaaaaaaga?agccgaaagc 180
agcatctacc?ggatagagtc?aaagcgggca?gcaaaaggag?ggctatacgc?ttttaaattg 240
atcagacctg?aaggccatcc?agatgttaaa?aagggcaaac?ctttcatttg?gtcaaatgta 300
atggagcttc?aagactgcaa?tgaagaaaca?ccgaagcctc?ctaaaaaagc?ttcagcaaaa 360
ccaaagcaaa?ctgaacagac?tcaaacaacg?caaaagtctg?aacaggatat?agaggaaaca 420
ccaaaggaca?gtgaatccgt?agataaagaa?aatagaaaag?aagataattc?attagaaagc 480
ggggaagcgt?catgaaacat?gtg?atg?aaa?tgg?atc?agc?aat?ttc?tta?tat?gtg 533
Val?Met?Lys?Trp?Ile?Ser?Asn?Phe?Leu?Tyr?Val
1 5 10
att?att?ttc?aca?atc?atc?atc?gcc?gcg?gtc?atc?gtc?gtg?att?tcg?aca 581
Ile?Ile?Phe?Thr?Ile?Ile?Ile?Ala?Ala?Val?Ile?Val?Val?Ile?Ser?Thr
15 20 25
aag?tcg?tcc?ggc?ggg?gag?ccg?cag?ctc?ttc?ggc?tat?caa?ttg?aaa?acg 629
Lys?Ser?Ser?Gly?Gly?Glu?Pro?Gln?Leu?Phe?Gly?Tyr?Gln?Leu?Lys?Thr
30 35 40
gtt?tta?tcc?gga?tcg?atg?gag?ccg?gag?ttt?aaa?aca?ggt?tct?gtg?att 677
Val?Leu?Ser?Gly?Ser?Met?Glu?Pro?Glu?Phe?Lys?Thr?Gly?Ser?Val?Ile
45 50 55
gcc?gtt?caa?aaa?gtt?gaa?aat?ccc?ggg?tct?ttg?aaa?aag?gga?gat?atc 725
Ala?Val?Gln?Lys?Val?Glu?Asn?Pro?Gly?Ser?Leu?Lys?Lys?Gly?Asp?Ile
60 65 70 75
att?aca?ttc?atg?caa?gac?gaa?aac?acc?atg?gtt?acc?cac?cga?att?atc 773
Ile?Thr?Phe?Met?Gln?Asp?Glu?Asn?Thr?Met?Val?Thr?His?Arg?Ile?Ile
80 85 90
ggt?ata?aca?aaa?aat?aaa?tca?aat?ctc?atg?ttc?aag?aca?aag?ggt?gac 821
Gly?Ile?Thr?Lys?Asn?Lys?Ser?Asn?Leu?Met?Phe?Lys?Thr?Lys?Gly?Asp
95 100 105
aat?aac?caa?aac?cot?gat?tcc?gat?ccg?gta?ctg?gcg?gaa?aat?gtt?gtc 869
Asn?Asn?Gln?Asn?Pro?Asp?Ser?Asp?Pro?Val?Leu?Ala?Glu?Asn?Val?Val
110 115 120
gct?aag?tat?tcg?ggc?att?acg?gtt?ccg?tat?gcc?ggg?tat?ttg?ctg?gac 917
Ala?Lys?Tyr?Ser?Gly?Ile?Thr?Val?Pro?Tyr?Ala?Gly?Tyr?Leu?Leu?Asp
125 130 135
ttt?gca?agt?aaa?ccg?atc?ggc?aca?gcc?att?ttg?ctg?atc?gtg?ccg?gga 965
Phe?Ala?Ser?Lys?Pro?Ile?Gly?Thr?Ala?Ile?Leu?Leu?Ile?Val?Pro?Gly
140 145 150 155
ctc?ttg?ttg?att?ctt?tat?gca?gta?att?act?gta?tcc?gcg?gct?tta?aga 1013
Leu?Leu?Leu?Ile?Leu?Tyr?Ala?Val?Ile?Thr?Val?Ser?Ala?Ala?Leu?Arg
160 165 170
gag?att?gac?caa?aaa?gct?aaa?gca?att?gaa?gcc?gct?gga?aaa?gat?caa 1061
Glu?Ile?Asp?Gln?Lys?Ala?Lys?Ala?Ile?Glu?Ala?Ala?Gly?Lys?Asp?Gln
175 180 185
tca?gtt?tcc?atg?taatcttcgt?tggtcacctg?aacattcagg?tctccatata 1113
Ser?Val?Ser?Met
190
tacagatttt?cttaattata?aaaggaggag?ttatcaatgg?gtacaaagaa?aaaactagga 1173
ttaggcgttg?cgtctgctgc?gcttggactg?gcattagtag?gaggaggaac?ttgggctgcg 1233
tttaacgaca?tcgaaacaac?tcaagcaact?tatgcagcag?gtacgcttga?cttaaatgcg 1293
aaagatacat?ctgcaagagt?gaacttgtcc?aacttaaaac?caggcgacaa?attcactaaa 1353
gatttcgagt?tcaaaaatga?cggatcactt?gcgattaaag?aagtgctgat?gcaggttggc 1413
tacagcaatt?tcgttgacgg?aaacgcgaaa?aacggcggaa?aaagcacagc?ggaagacttc 1473
ctgaaacaat?ttaaagtcag?cgttctgact?gtcggagttg?aaggcggtaa?cggctatcct 1533
aaaaacatca?ttttagatga?agccaacctt?tatgatctgt?aca 1576
<210>48
<211>191
<212>PRT
＜213〉Bacillus licheniformis
<400>48
Val?Met?Lys?Trp?Ile?Ser?Asn?Phe?Leu?Tyr?Val?Ile?Ile?Phe?Thr?Ile
1 5 10 15
Ile?Ile?Ala?Ala?Val?Ile?Val?Val?Ile?Ser?Thr?Lys?Ser?Ser?Gly?Gly
20 25 30
Glu?Pro?Gln?Leu?Phe?Gly?Tyr?Gln?Leu?Lys?Thr?Val?Leu?Ser?Gly?Ser
35 40 45
Met?Glu?Pro?Glu?Phe?Lys?Thr?Gly?Ser?Val?Ile?Ala?Val?Gln?Lys?Val
50 55 60
Glu?Asn?Pro?Gly?Ser?Leu?Lys?Lys?Gly?Asp?Ile?Ile?Thr?Phe?Met?Gln
65 70 75 80
Asp?Glu?Asn?Thr?Met?Val?Thr?His?Arg?Ile?Ile?Gly?Ile?Thr?Lys?Asn
85 90 95
Lys?Ser?Asn?Leu?Met?Phe?Lys?Thr?Lys?Gly?Asp?Asn?Asn?Gln?Asn?Pro
100 105 110
Asp?Ser?Asp?Pro?Val?Leu?Ala?Glu?Asn?Val?Val?Ala?Lys?Tyr?Ser?Gly
115 120 125
Ile?Thr?Val?Pro?Tyr?Ala?Gly?Tyr?Leu?Leu?Asp?Phe?Ala?Ser?Lys?Pro
130 135 140
Ile?Gly?Thr?Ala?Ile?Leu?Leu?Ile?Val?Pro?Gly?Leu?Leu?Leu?Ile?Leu
145 150 155 160
Tyr?Ala?Val?Ile?Thr?Val?Ser?Ala?Ala?Leu?Arg?Glu?Ile?Asp?Gln?Lys
165 170 175
Ala?Lys?Ala?Ile?Glu?Ala?Ala?Gly?Lys?Asp?Gln?Ser?Val?Ser?Met
180 185 190
<210>49
<211>1514
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1016)
<400>49
tgcccctgct?cgtgtttgtt?gttgccaatg?taaattcgtt?cggtttatgg?caggggtttt 60
tgttgacgtg?ggtcggggca?tctttaggtt?ctatagcggt?ttttcttttt?atccgccatt 120
tcggccagca?aaaagcactc?ggttttatcc?gcaggcatcc?atccgtaaaa?aaactgatga 180
tctgggtgga?aaaacggggc?ttcggtccgc?tgtttctcct?gctttgcttt?ccattcacgc 240
cttcggcggc?ggtaaatgtt?gtggcaggac?tttcgcgagt?cagcctgctg?cctttttcat 300
tggcggtgct?ttgcggcaag?ttcgtgatga?tttttatggt?gagctttgtc?ggccatgatc 360
tgaaggcgct?ttttacccag?cctttgcgga?cagccgccgt?gatcatcgtc?atgctgatct 420
tatggtacgc?cggaaaacgg?gttgaacatc?gtctgaacgt?gagattcagc?cagagagaga 480
aagactaggg?ggacacagaa?ttg?aaa?aag?cgt?ttt?ttg?gtc?ata?gcc?ggg?gta 533
Leu?Lys?Lys?Arg?Phe?Leu?Val?Ile?Ala?Gly?Val
1 5 10
ctg?ctt?gtc?atc?gtc?gtc?ggg?att?cag?gtc?aaa?aat?gcc?gtt?ttc?att 581
Leu?Leu?Val?Ile?Val?Val?Gly?Ile?Gln?Val?Lys?Asn?Ala?Val?Phe?Ile
15 20 25
gaa?tat?aaa?gta?gaa?ggc?gtc?agc?atg?aat?ccg?acc?ttt?cag?gag?ggc 629
Glu?Tyr?Lys?Val?Glu?Gly?Val?Ser?Met?Asn?Pro?Thr?Phe?Gln?Glu?Gly
30 35 40
aac?gag?ctt?ttg?atc?aac?agg?ttc?gcc?cac?cgc?ttt?aaa?acg?atc?agc 677
Asn?Glu?Leu?Leu?Ile?Asn?Arg?Phe?Ala?His?Arg?Phe?Lys?Thr?Ile?Ser
45 50 55
cga?ttt?gac?atc?gtg?ctg?ttt?aaa?gga?cct?gac?aaa?gat?ata?ttc?att 725
Arg?Phe?Asp?Ile?Val?Leu?Phe?Lys?Gly?Pro?Asp?Lys?Asp?Ile?Phe?Ile
60 65 70 75
aaa?agg?gtg?atc?ggg?ctt?ccg?ggc?gaa?acc?ctc?agg?tat?gaa?gat?gat 773
Lys?Arg?Val?Ile?Gly?Leu?Pro?Gly?Glu?Thr?Leu?Arg?Tyr?Glu?Asp?Asp
80 85 90
cag?ctg?tat?atc?aac?gaa?gaa?aag?atc?aaa?gag?cct?tat?ctg?gac?gac 821
Gln?Leu?Tyr?Ile?Asn?Glu?Glu?Lys?Ile?Lys?Glu?Pro?Tyr?Leu?Asp?Asp
95 100 105
tta?aag?gcc?gtc?acc?gcc?gga?ggg?gac?ttg?aca?ggg?gat?ttt?aca?ctg 869
Leu?Lys?Ala?Val?Thr?Ala?Gly?Gly?Asp?Leu?Thr?Gly?Asp?Phe?Thr?Leu
110 115 120
cag?gaa?gtg?acc?gga?gag?gag?aag?gtg?cct?gaa?aac?gag?tac?ttc?gtc 917
Gln?Glu?Val?Thr?Gly?Glu?Glu?Lys?Val?Pro?Glu?Asn?Glu?Tyr?Phe?Val
125 130 135
ctc?ggg?gac?aac?cgg?atc?cac?agc?ttt?gac?agc?cgc?cat?ttc?ggc?ttt 965
Leu?Gly?Asp?Asn?Arg?Ile?His?Ser?Phe?Asp?Ser?Arg?His?Phe?Gly?Phe
140 145 150 155
gtt?tca?gaa?cgg?gac?atc?gtc?ggg?att?gtg?acg?gaa?aga?att?gat?aag 1013
Val?Ser?Glu?Arg?Asp?Ile?Val?Gly?Ile?Val?Thr?Glu?Arg?Ile?Asp?Lys
160 165 170
aag?tgat?tggaga?gtacggggga?gagtaagcgg?ccgaccaagg?aatacgatta 1066
Lys
cgcaaatgac?gagcccgaaa?tgtcaattag?tacaacagca?tcaataatga?cgcatttgct 1126
aaatatgaaa?attaaaaggc?ccggatgatt?ccgggctttt?ttccgtacta?cttcagctta 1186
atggcattga?tccgtttttg?cagcttcgtt?ttttcggcgg?atgctttcag?cttgcctact 1246
gctgattgcg?ctgctgattt?tgtttttttc?gttctctttt?gttcggccgt?tttgactttg 1306
gcctttgctg?cagaaacttt?tttattatag?gctgcagctg?cttgcttttt?cgcggtgttc 1366
agcctttttt?gcagatttga?tttgtctttt?ccggcgggaa?gctcattgat?cgctttttgc 1426
gctttgccga?ggtttgagtc?tgttgccgct?ttttcagctt?tggcaacggc?ctgctttgcg 1486
gtcttcaaga?ggcttgaacg?gaccgcgt 1514
<210>50
<211>172
<212>PRT
＜213〉Bacillus licheniformis
<400>50
Leu?Lys?Lys?Arg?Phe?Leu?Val?Ile?Ala?Gly?Val?Leu?Leu?Val?Ile?Val
1 5 10 15
Val?Gly?Ile?Gln?Val?Lys?Asn?Ala?Val?Phe?Ile?Glu?Tyr?Lys?Val?Glu
20 25 30
Gly?Val?Ser?Met?Asn?Pro?Thr?Phe?Gln?Glu?Gly?Asn?Glu?Leu?Leu?Ile
35 40 45
Asn?Arg?Phe?Ala?His?Arg?Phe?Lys?Thr?Ile?Ser?Arg?Phe?Asp?Ile?Val
50 55 60
Leu?Phe?Lys?Gly?Pro?Asp?Lys?Asp?Ile?Phe?Ile?Lys?Arg?Val?Ile?Gly
65 70 75 80
Leu?Pro?Gly?Glu?Thr?Leu?Arg?Tyr?Glu?Asp?Asp?Gln?Leu?Tyr?Ile?Asn
85 90 95
Glu?Glu?Lys?Ile?Lys?Glu?Pro?Tyr?Leu?Asp?Asp?Leu?Lys?Ala?Val?Thr
100 105 110
Ala?Gly?Gly?Asp?Leu?Thr?Gly?Asp?Phe?Thr?Leu?Gln?Glu?Val?Thr?Gly
115 120 125
Glu?Glu?Lys?Val?Pro?Glu?Asn?Glu?Tyr?Phe?Val?Leu?Gly?Asp?Asn?Arg
130 135 140
Ile?His?Ser?Phe?Asp?Ser?Arg?His?Phe?Gly?Phe?Val?Ser?Glu?Arg?Asp
145 150 155 160
Ile?Val?Gly?Ile?Val?Thr?Glu?Arg?Ile?Asp?Lys?Lys
165 170
<210>51
<211>1081
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(1)..(585)
<400>51
aaa?cag?ctg?tgg?tgc?atc?ttt?tta?acc?ctc?gga?gtc?aga?agt?ttc?ggc 48
Lys?Gln?Leu?Trp?Cys?Ile?Phe?Leu?Thr?Leu?Gly?Val?Arg?Ser?Phe?Gly
1 5 10 15
ctg?aca?tgg?aac?tgg?gcc?aat?acg?ttc?gca?gac?ggc?gct?tta?gaa?gaa 96
Leu?Thr?Trp?Asn?Trp?Ala?Asn?Thr?Phe?Ala?Asp?Gly?Ala?Leu?Glu?Glu
20 25 30
aga?aac?gcc?ggc?ttg?act?tct?tac?ggg?gaa?aga?ctg?att?cgc?aag?gcg 144
Arg?Asn?Ala?Gly?Leu?Thr?Ser?Tyr?Gly?Glu?Arg?Leu?Ile?Arg?Lys?Ala
35 40 45
aac?aaa?aac?cgg?gca?tgg?aca?gat?ctc?tcc?cat?tta?tcc?gaa?aaa?agc 192
Asn?Lys?Asn?Arg?Ala?Trp?Thr?Asp?Leu?Ser?His?Leu?Ser?Glu?Lys?Ser
50 55 60
ttt?tgg?ggt?gct?att?gaa?acg?gcg?gcg?tat?ccg?atc?gcc?tct?cat?tcg 240
Phe?Trp?Gly?Ala?Ile?Glu?Thr?Ala?Ala?Tyr?Pro?Ile?Ala?Ser?His?Ser
65 70 75 80
aat?gcc?cgc?agc?ctt?tgc?cct?cat?ccg?aga?aat?tta?aaa?gac?gat?caa 288
Asn?Ala?Arg?Ser?Leu?Cys?Pro?His?Pro?Arg?Asn?Leu?Lys?Asp?Asp?Gln
85 90 95
atc?aaa?gcg?ctt?att?gat?aag?aac?ggc?gtc?atc?ggc?ttg?aca?ttt?gtc 336
Ile?Lys?Ala?Leu?Ile?Asp?Lys?Asn?Gly?Val?Ile?Gly?Leu?Thr?Phe?Val
100 105 110
cct?gaa?ttc?gtg?aga?aac?gga?aaa?acg?cct?gtg?atc?aaa?gat?atc?ctc 384
Pro?Glu?Phe?Val?Arg?Asn?Gly?Lys?Thr?Pro?Val?Ile?Lys?Asp?Ile?Leu
115 120 125
tcg?cat?att?gac?cat?gtc?tgc?tct?tta?cga?ggc?gag?cgg?cat?atc?gga 432
Ser?His?Ile?Asp?His?Val?Cys?Ser?Leu?Arg?Gly?Glu?Arg?His?Ile?Gly
130 135 140
ttc?ggg?tct?gat?ttt?gac?ggc?atc?gac?cgt?gtc?att?ccg?aat?ctt?gaa 480
Phe?Gly?Ser?Asp?Phe?Asp?Gly?Ile?Asp?Arg?Val?Ile?Pro?Asn?Leu?Glu
145 150 155 160
gcc?cac?aaa?gat?tac?ggg?aat?ctg?atc?gaa?gcg?ctg?cag?cgc?tca?tat 528
Ala?His?Lys?Asp?Tyr?Gly?Asn?Leu?Ile?Glu?Ala?Leu?Gln?Arg?Ser?Tyr
165 170 175
aca?cca?agt?caa?gtc?gac?gga?ttt?ctc?ttt?caa?aat?ttc?atc?agc?cgc 576
Thr?Pro?Ser?Gln?Val?Asp?Gly?Phe?Leu?Phe?Gln?Asn?Phe?Ile?Ser?Arg
180 185 190
atc?cca?ttt?taaaaaaata?tcatacattt?ttcagagggt?gttcatctgt 625
Ile?Pro?Phe
195
tttctccaga?aggacactgg?tgtttaccaa?gactgattat?cgtgctacaa?tccttttgta 685
aagcgatttt?acaaaggggg?tctgttcatc?ttggacggaa?atatgaaagc?gcttatcaag 745
aagccggggg?agccgggcgc?cagcttcgag?ctcgtcccga?ttccgaagat?cgacaaacac 805
gatgtcctga?tccaagtcac?agccgcatcg?atttgcggaa?acgatgtcca?tatttataat 865
tgggcatgaa?tgcgccacac?acctccggta?aacccgacct?aatgtctccg?gacaccgaac 925
tttctcggcc?tacaatcctt?ccaatgcacc?ccacatccgt?ccaacaactc?cccccagaac 985
ccacctagca?ttccccacac?cccccccact?acccttttcg?ccccatcacc?acctcccccc 1045
cctcccccta?atattatccc?tcctccatag?aaattt 1081
<210>52
<211>195
<212>PRT
＜213〉Bacillus licheniformis
<400>52
Lys?Gln?Leu?Trp?Cys?Ile?Phe?Leu?Thr?Leu?Gly?Val?Arg?Ser?Phe?Gly
1 5 10 15
Leu?Thr?Trp?Asn?Trp?Ala?Asn?Thr?Phe?Ala?Asp?Gly?Ala?Leu?Glu?Glu
20 25 30
Arg?Asn?Ala?Gly?Leu?Thr?Ser?Tyr?Gly?Glu?Arg?Leu?Ile?Arg?Lys?Ala
35 40 45
Asn?Lys?Asn?Arg?Ala?Trp?Thr?Asp?Leu?Ser?His?Leu?Ser?Glu?Lys?Ser
50 55 60
Phe?Trp?Gly?Ala?Ile?Glu?Thr?Ala?Ala?Tyr?Pro?Ile?Ala?Ser?His?Ser
65 70 75 80
Asn?Ala?Arg?Ser?Leu?Cys?Pro?His?Pro?Arg?Asn?Leu?Lys?Asp?Asp?Gln
85 90 95
Ile?Lys?Ala?Leu?Ile?Asp?Lys?Asn?Gly?Val?Ile?Gly?Leu?Thr?Phe?Val
100 105 110
Pro?G1u?Phe?Val?Arg?Asn?Gly?Lys?Thr?Pro?Val?Ile?Lys?Asp?Ile?Leu
115 120 125
Ser?His?Ile?Asp?His?Val?Cys?Ser?Leu?Arg?G1y?Glu?Arg?His?Ile?Gly
130 135 140
Phe?Gly?Ser?Asp?Phe?Asp?G1y?11e?Asp?Arg?Val?Ile?Pro?Asn?Leu?Glu
145 150 155 160
Ala?His?Lys?Asp?Tyr?Gly?Asn?Leu?Ile?Glu?Ala?Leu?Gln?Arg?Ser?Tyr
165 170 175
Thr?Pro?Ser?Gln?Val?Asp?Gly?Phe?Leu?Phe?Gln?Asn?Phe?Ile?Ser?Arg
180 185 190
Ile?Pro?Phe
195
<210>53
<211>3560
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(3122)
<400>53
aagaaggaaa?agccgaatta?aagcttggcc?atgccgaaca?aattccttat?ccggatcgtt 60
cttttcataa?agtattttca?gtccacacca?tttatttttg?gacggatttc?aaacaggcat 120
taaaagaaat?ttaccgtgtt?ttacaggttg?acggcacgct?gtttctcgct?gtgcatctgg 180
aagggcaaat?gaaaaaatcg?aaaaaaacaa?aaggcttttc?cttatactca?gaagagcaaa 240
tcaagcaact?gcttgaggaa?agccatttca?gagacatcac?ggtacatatg?aacaaaaatt 300
actgctgcat?ttctgcggta?aaatcatgaa?ctttgtatga?tcatcccttt?caatacggaa 360
gggatttttt?atgtttgata?gagttgaaac?tggatcttaa?atatcatatt?tttgattttt 420
aaagaaaagt?attccattaa?catagcaaac?atggtttaat?atcaaagtga?acgtttttac 480
tatattttcc?ggaggtattt?atg?aac?aaa?agg?atc?gtg?aaa?agt?tca?att?gtt 533
Met?Asn?Lys?Arg?Ile?Val?Lys?Ser?Ser?Ile?Val
1 5 10
ttc?ttt?ctc?ctt?gcc?gca?tta?att?ttt?ggc?cag?ctg?cct?tta?ccg?aag 581
Phe?Phe?Leu?Leu?Ala?Ala?Leu?Ile?Phe?Gly?Gln?Leu?Pro?Leu?Pro?Lys
15 20 25
aca?atg?gca?gcg?gaa?gac?agc?gtt?ccg?aat?aac?gaa?acc?aca?ttg?acc 629
Thr?Met?Ala?Ala?Glu?Asp?Ser?Val?Pro?Asn?Asn?Glu?Thr?Thr?Leu?Thr
30 35 40
agc?gcg?tcc?cct?gtt?gaa?gct?tcg?ttt?caa?agc?gat?gac?gag?gtg?cat 677
Ser?Ala?Ser?Pro?Val?Glu?Ala?Ser?Phe?Gln?Ser?Asp?Asp?Glu?Val?His
45 50 55
tgg?tat?aaa?gtc?aat?cct?tca?aat?cag?gaa?atc?gca?aac?tat?acg?cac 725
Trp?Tyr?Lys?Val?Asn?Pro?Ser?Asn?Gln?Glu?Ile?Ala?Asn?Tyr?Thr?His
60 65 70 75
ttc?cgc?gtc?aaa?ttg?aaa?tca?gat?gca?gag?ctg?aac?att?tcc?gtc?tac 773
Phe?Arg?Val?Lys?Leu?Lys?Ser?Asp?Ala?Glu?Leu?Asn?Ile?Ser?Val?Tyr
80 85 90
tcc?agc?ctg?gaa?aat?gca?act?ggt?cat?caa?acg?ttt?gac?cga?tac?aac 821
Ser?Ser?Leu?Glu?Asn?Ala?Thr?Gly?His?Gln?Thr?Phe?Asp?Arg?Tyr?Asn
95 100 105
ggc?tac?tcc?tat?gaa?aat?aat?cct?gct?tta?atc?gat?ttt?ccg?att?gcc 869
Gly?Tyr?Ser?Tyr?Glu?Asn?Asn?Pro?Ala?Leu?Ile?Asp?Phe?Pro?Ile?Ala
110 115 120
tgg?aaa?ggt?cct?tac?tac?ata?aaa?gta?gaa?aat?cac?cat?gat?gag?gaa 917
Trp?Lys?Gly?Pro?Tyr?Tyr?Ile?Lys?Val?Glu?Asn?His?His?Asp?Glu?Glu
125 130 135
aac?gaa?acc?act?tca?att?aca?gat?att?tct?tac?acc?atc?agc?tat?gaa 965
Asn?Glu?Thr?Thr?Ser?Ile?Thr?Asp?Ile?Ser?Tyr?Thr?Ile?Ser?Tyr?Glu
140 145 150 155
ggc?gtc?acc?ctg?cct?ccg?tca?atc?caa?gag?gca?gaa?gaa?gag?tgt?ccg 1013
Gly?Val?Thr?Leu?Pro?Pro?Ser?Ile?Gln?Glu?Ala?Glu?Glu?Glu?Cys?Pro
160 165 170
gca?gaa?tta?agc?gtt?tcc?gaa?agg?gaa?aca?ggc?aag?ggc?ata?tta?aaa 1061
Ala?Glu?Leu?Ser?Val?Ser?Glu?Arg?Glu?Thr?Gly?Lys?Gly?Ile?Leu?Lys
175 180 185
cag?tta?aga?acg?atc?agg?gat?gaa?gtt?ctt?tca?aaa?act?gaa?aaa?gga 1109
Gln?Leu?Arg?Thr?Ile?Arg?Asp?Glu?Val?Leu?Ser?Lys?Thr?Glu?Lys?Gly
190 195 200
aaa?gag?ctg?tct?tcc?ctt?tac?tat?aaa?gca?gct?cca?ttc?atc?agc?gca 1157
Lys?Glu?Leu?Ser?Ser?Leu?Tyr?Tyr?Lys?Ala?Ala?Pro?Phe?Ile?Ser?Ala
205 210 215
aaa?atg?ctc?ttt?aac?aaa?tcg?atg?aga?gac?agt?gtc?tac?aaa?gac?ctg 1205
Lys?Met?Leu?Phe?Asn?Lys?Ser?Met?Arg?Asp?Ser?Val?Tyr?Lys?Asp?Leu
220 225 230 235
gtg?cag?ctg?aag?ccg?ctg?ttt?gca?gat?gtc?gct?aaa?aac?gga?caa?gtg 1253
Val?Gln?Leu?Lys?Pro?Leu?Phe?Ala?Asp?Val?Ala?Lys?Asn?Gly?Gln?Val
240 245 250
agc?gca?tac?tcg?att?acg?aat?gat?gat?caa?aaa?gcg?atc?agt?cgc?ctg 1301
Ser?Ala?Tyr?Ser?Ile?Thr?Asn?Asp?Asp?Gln?Lys?Ala?Ile?Ser?Arg?Leu
255 260 265
tac?gaa?aca?gct?cgc?gcg?tcc?gtt?ccc?gag?ccg?ttg?aaa?aaa?cag?ctg 1349
Tyr?Glu?Thr?Ala?Arg?Ala?Ser?Val?Pro?Glu?Pro?Leu?Lys?Lys?Gln?Leu
270 275 280
gat?caa?gtc?gcg?aaa?gac?atc?ggc?atc?gaa?caa?tta?aca?ggc?agc?aaa 1397
Asp?Gln?Val?Ala?Lys?Asp?Ile?Gly?Ile?Glu?Gln?Leu?Thr?Gly?Ser?Lys
285 290 295
gta?tcg?gct?gtg?ctt?gaa?aaa?gcg?ggg?atg?gcg?aca?gct?tca?tca?agc 1445
Val?Ser?Ala?Val?Leu?Glu?Lys?Ala?Gly?Met?Ala?Thr?Ala?Ser?Ser?Ser
300 305 310 315
gcg?ccc?gaa?aac?cgt?tac?atc?gta?aaa?ttg?aaa?gaa?ggc?aaa?aaa?ccg 1493
Ala?Pro?Glu?Asn?Arg?Tyr?Ile?Val?Lys?Leu?Lys?Glu?Gly?Lys?Lys?Pro
320 325 330
gga?tct?ttc?aaa?tct?aaa?gcc?caa?tca?tcc?ggc?gtc?cag?gca?tta?gag 1541
Gly?Ser?Phe?Lys?Ser?Lys?Ala?Gln?Ser?Ser?Gly?Val?Gln?Ala?Leu?Glu
335 340 345
ccc?ctc?ggt?aaa?agc?aaa?acg?gca?ttt?aaa?gat?atg?tac?gtt?gtg?gaa 1589
Pro?Leu?Gly?Lys?Ser?Lys?Thr?Ala?Phe?Lys?Asp?Met?Tyr?Val?Val?Glu
350 355 360
atg?aag?gaa?agc?cgt?tct?tcc?gga?ttc?aaa?gcg?gcg?gca?aag?caa?tat 1637
Met?Lys?Glu?Ser?Arg?Ser?Ser?Gly?Phe?Lys?Ala?Ala?Ala?Lys?Gln?Tyr
365 370 375
cag?gcg?gca?gcc?tcc?aag?atc?gcc?aag?atg?cct?gaa?gtg?gaa?ttc?gtc 1685
Gln?Ala?Ala?Ala?Ser?Lys?Ile?Ala?Lys?Met?Pro?Glu?Val?Glu?Phe?Val
380 385 390 395
gaa?cag?gtt?cag?caa?tat?gaa?gca?ctg?tca?aga?gac?acc?caa?tat?cca 1733
Glu?Gln?Val?Gln?Gln?Tyr?Glu?Ala?Leu?Ser?Arg?Asp?Thr?Gln?Tyr?Pro
400 405 410
tat?caa?tgg?tcg?ctc?aaa?aat?aac?ggc?aaa?aac?cgt?gct?gcg?aat?gct 1781
Tyr?Gln?Trp?Ser?Leu?Lys?Asn?Asn?Gly?Lys?Asn?Arg?Ala?Ala?Asn?Ala
415 420 425
gac?ata?caa?ttt?gaa?cag?ctt?cag?aag?ctg?atg?aaa?ggc?aaa?aag?ctg 1829
Asp?Ile?Gln?Phe?Glu?Gln?Leu?Gln?Lys?Leu?Met?Lys?Gly?Lys?Lys?Leu
430 435 440
aaa?gat?aca?gta?atc?gcc?gtc?gtt?gac?aca?ggc?gtt?gat?cat?acc?ctt 1877
Lys?Asp?Thr?Val?Ile?Ala?Val?Val?Asp?Thr?Gly?Val?Asp?His?Thr?Leu
445 450 455
gcg?gat?tta?agc?ggc?agc?gtc?aaa?aaa?gac?gaa?ggc?tat?aac?tat?gtc 1925
Ala?Asp?Leu?Ser?Gly?Ser?Val?Lys?Lys?Asp?Glu?Gly?Tyr?Asn?Tyr?Val
460 465 470 475
ggc?cgc?acg?gcg?gat?gcg?atg?gat?gac?aat?ggc?cac?ggc?aca?cac?gtg 1973
Gly?Arg?Thr?Ala?Asp?Ala?Met?Asp?Asp?Asn?Gly?His?Gly?Thr?His?Val
480 485 490
tca?ggc?atc?att?gca?gcc?gcg?caa?gac?aac?cat?ttt?tcg?atg?gcg?gga 2021
Ser?Gly?Ile?Ile?Ala?Ala?Ala?Gln?Asp?Asn?His?Phe?Ser?Met?Ala?Gly
495 500 505
atc?aat?gct?tat?gcc?aaa?att?ctg?cct?gtc?aaa?gtg?ctg?gat?tct?tca 2069
Ile?Asn?Ala?Tyr?Ala?Lys?Ile?Leu?Pro?Val?Lys?Val?Leu?Asp?Ser?Ser
510 515 520
ggc?agt?gga?gat?acg?gaa?cag?att?gca?aac?ggc?atc?atc?tat?gcc?gcc 2117
Gly?Ser?Gly?Asp?Thr?Glu?Gln?Ile?Ala?Asn?Gly?Ile?Ile?Tyr?Ala?Ala
525 530 535
gac?cac?ggt?gca?aaa?gtc?atc?aat?tta?agt?ctt?ggc?ggg?cca?tac?agc 2165
Asp?His?Gly?Ala?Lys?Val?Ile?Asn?Leu?Ser?Leu?Gly?Gly?Pro?Tyr?Ser
540 545 550 555
cgg?gtg?atg?gaa?tat?gcg?ctt?aaa?tat?gcg?gct?tct?aaa?aat?gtg?acg 2213
Arg?Val?Met?Glu?Tyr?Ala?Leu?Lys?Tyr?Ala?Ala?Ser?Lys?Asn?Val?Thr
560 565 570
atc?gtt?gcc?gcc?acc?gga?aat?gac?gga?gta?tcg?gag?att?tcc?tac?cct 2261
Ile?Val?Ala?Ala?Thr?Gly?Asn?Asp?Gly?Val?Ser?Glu?Ile?Ser?Tyr?Pro
575 580 585
gca?tct?tcg?aaa?tat?acg?ctt?tca?gtc?ggg?gcg?acc?aat?aat?ctc?gat 2309
Ala?Ser?Ser?Lys?Tyr?Thr?Leu?Ser?Val?Gly?Ala?Thr?Asn?Asn?Leu?Asp
590 595 600
ctt?gtc?tcg?gac?tac?tcc?aat?tat?gga?aaa?ggt?ctc?gat?atg?gtg?gcg 2357
Leu?Val?Ser?Asp?Tyr?Ser?Asn?Tyr?Gly?Lys?Gly?Leu?Asp?Met?Val?Ala
605 610 615
ccg?gga?acc?gat?att?cca?agc?ctc?gtt?ccg?gac?ggg?aat?gtc?act?tat 2405
Pro?Gly?Thr?Asp?Ile?Pro?Ser?Leu?Val?Pro?Asp?Gly?Asn?Val?Thr?Tyr
620 625 630 635
atg?agc?gga?aca?tcg?atg?gcg?gcg?ccg?cac?gtg?gca?gct?gca?gca?gga 2453
Met?Ser?Gly?Thr?Ser?Met?Ala?Ala?Pro?His?Val?Ala?Ala?Ala?Ala?Gly
640 645 650
ctt?ctt?ttg?tca?cag?aat?ccg?tcc?ttg?aaa?cca?aag?caa?atc?gca?agc 2501
Leu?Leu?Leu?Ser?Gln?Asn?Pro?Ser?Leu?Lys?Pro?Lys?Gln?Ile?Ala?Ser
655 660 665
cta?ttg?acc?gag?acg?aca?gca?gat?gtg?gca?ttt?gaa?gag?cag?gat?aat 2549
Leu?Leu?Thr?Glu?Thr?Thr?Ala?Asp?Val?Ala?Phe?Glu?Glu?Gln?Asp?Asn
670 675 680
cca?aac?ccg?gat?tat?gac?ctg?gat?ata?gaa?ccg?gct?gca?caa?att?ccc 2597
Pro?Asn?Pro?Asp?Tyr?Asp?Leu?Asp?Ile?Glu?Pro?Ala?Ala?Gln?Ile?Pro
685 690 695
gga?tat?gac?ttc?gtc?tcc?ggg?tgg?gga?agg?ctg?aat?gtt?ttt?cat?gca 2645
Gly?Tyr?Asp?Phe?Val?Ser?Gly?Trp?Gly?Arg?Leu?Asn?Val?Phe?His?Ala
700 705 710 715
gcc?agc?gtt?ttt?gag?ctg?aac?atg?aag?gtt?cat?ccc?gtt?tta?aac?cgc 2693
Ala?Ser?Val?Phe?Glu?Leu?Asn?Met?Lys?Val?His?Pro?Val?Leu?Asn?Arg
720 725 730
cat?acg?gca?gtg?aca?ggc?aca?gcc?aaa?agc?ggt?gtg?acg?gtc?aaa?atc 2741
His?Thr?Ala?Val?Thr?Gly?Thr?Ala?Lys?Ser?Gly?Val?Thr?Val?Lys?Ile
735 740 745
ttg?cga?ggg?aag?caa?gta?ttg?ggg?acg?ggc?acg?gcc?gga?aaa?tca?ggc 2789
Leu?Arg?Gly?Lys?Gln?Val?Leu?Gly?Thr?Gly?Thr?Ala?Gly?Lys?Ser?Gly
750 755 760
gcg?ttt?tca?gtg?aaa?att?ccg?gcc?cag?aag?gcg?ggg?caa?gtt?ctt?cat 2837
Ala?Phe?Ser?Val?Lys?Ile?Pro?Ala?Gln?Lys?Ala?Gly?Gln?Val?Leu?His
765 770 775
gtc?gcg?gca?tcg?ggc?cat?cag?gcg?gaa?acc?tcg?ctc?aga?acc?gtc?gtg 2885
Val?Ala?Ala?Ser?Gly?His?Gln?Ala?Glu?Thr?Ser?Leu?Arg?Thr?Val?Val
780 785 790 795
gaa?aaa?gcg?ccg?aaa?aac?ccg?tcc?gtc?aaa?cgc?atc?acg?aac?aaa?gat 2933
Glu?Lys?Ala?Pro?Lys?Asn?Pro?Ser?Val?Lys?Arg?Ile?Thr?Asn?Lys?Asp
800 805 810
act?gcc?gta?acg?ggt?aga?acg?gca?gcc?ggc?tac?acg?atc?aaa?gtg?aaa 2981
Thr?Ala?Val?Thr?Gly?Arg?Thr?Ala?Ala?Gly?Tyr?Thr?Ile?Lys?Val?Lys
815 820 825
aac?gcg?tgc?aaa?aaa?gtg?atc?gcg?caa?ggc?aga?gcg?gat?gca?tcc?gtg 3029
Asn?Ala?Cys?Lys?Lys?Val?Ile?Ala?Gln?Gly?Arg?Ala?Asp?Ala?Ser?Val
830 835 840
agc?gtt?aaa?gtg?aaa?atc?aac?aag?caa?aaa?gat?atg?ccg?ttt?tgt?atg 3077
Ser?Val?Lys?Val?Lys?Ile?Asn?Lys?Gln?Lys?Asp?Met?Pro?Phe?Cys?Met
845 850 855
tct?ccg?cat?ctg?ctg?atg?acc?aca?gag?aaa?gcg?gcg?atg?tca?aaa 3122
Ser?Pro?His?Leu?Leu?Met?Thr?Thr?Glu?Lys?Ala?Ala?Met?Ser?Lys
860 865 870
tgacggtggc?tgacgtcatc?ccgccaggcg?ccccgaaagt?ttatcaggtt?tccgataaaa 3182
gtacggtgat?tcagggaaaa?acagaagcaa?acgcgcaagt?cagcgcaaaa?gctaaaggaa 3242
agacgatcgc?ttccggcaaa?gcgaacggaa?aaggtgaata?caagctgaaa?atcagcaggc 3302
aaaaagccgg?aaccgtcatc?ggtgtaacag?caaaggataa?agccggaaat?gtcagcaaag 3362
cgacagccgt?cactgtgctt?gacaaaaccc?cgccgtctgc?gccgaaggtg?aatccggtga 3422
caaacaagag?cacggccgtt?aaggggaaag?cagaagcgaa?cgccgccatc?atcgtcaaat 3482
caggaaagaa?aacgatcgga?accggcaagg?ccgataaaaa?aggcgcgttt?tttgtcaaaa 3542
taaaaaaaca?aaaggcaa 3560
<210>54
<211>874
<212>PRT
＜213〉Bacillus licheniformis
<400>54
Met?Asn?Lys?Arg?Ile?Val?Lys?Ser?Ser?Ile?Val?Phe?Phe?Leu?Leu?Ala
1 5 10 15
Ala?Leu?Ile?Phe?Gly?Gln?Leu?Pro?Leu?Pro?Lys?Thr?Met?Ala?Ala?Glu
20 25 30
Asp?Ser?Val?Pro?Asn?Asn?Glu?Thr?Thr?Leu?Thr?Ser?Ala?Ser?Pro?Val
35 40 45
Glu?Ala?Ser?Phe?Gln?Ser?Asp?Asp?Glu?Val?His?Trp?Tyr?Lys?Val?Asn
50 55 60
Pro?Ser?Asn?Gln?Glu?Ile?Ala?Asn?Tyr?Thr?His?Phe?Arg?Val?Lys?Leu
65 70 75 80
Lys?Ser?Asp?Ala?Glu?Leu?Asn?Ile?Ser?Val?Tyr?Ser?Ser?Leu?Glu?Asn
85 90 95
Ala?Thr?Gly?His?Gln?Thr?Phe?Asp?Arg?Tyr?Asn?Gly?Tyr?Ser?Tyr?Glu
100 105 110
Asn?Asn?Pro?Ala?Leu?Ile?Asp?Phe?Pro?Ile?Ala?Trp?Lys?Gly?Pro?Tyr
115 120 125
Tyr?Ile?Lys?Val?Glu?Asn?His?His?Asp?Glu?Glu?Asn?Glu?Thr?Thr?Ser
130 135 140
Ile?Thr?Asp?Ile?Ser?Tyr?Thr?Ile?Ser?Tyr?Glu?Gly?Val?Thr?Leu?Pro
145 150 155 160
Pro?Ser?Ile?Gln?Glu?Ala?Glu?Glu?Glu?Cys?Pro?Ala?Glu?Leu?Ser?Val
165 170 175
Ser?Glu?Arg?Glu?Thr?Gly?Lys?Gly?Ile?Leu?Lys?Gln?Leu?Arg?Thr?Ile
180 185 190
Arg?Asp?Glu?Val?Leu?Ser?Lys?Thr?Glu?Lys?Gly?Lys?Glu?Leu?Ser?Ser
195 200 205
Leu?Tyr?Tyr?Lys?Ala?Ala?Pro?Phe?Ile?Ser?Ala?Lys?Met?Leu?Phe?Asn
210 215 220
Lys?Ser?Met?Arg?Asp?Ser?Val?Tyr?Lys?Asp?Leu?Val?Gln?Leu?Lys?Pro
225 230 235 240
Leu?Phe?Ala?Asp?Val?Ala?Lys?Asn?Gly?Gln?Val?Ser?Ala?Tyr?Ser?Ile
245 250 255
Thr?Asn?Asp?Asp?Gln?Lys?Ala?Ile?Ser?Arg?Leu?Tyr?Glu?Thr?Ala?Arg
260 265 270
Ala?Ser?Val?Pro?Glu?Pro?Leu?Lys?Lys?Gln?Leu?Asp?Gln?Val?Ala?Lys
275 280 285
Asp?Ile?Gly?Ile?Glu?Gln?Leu?Thr?Gly?Ser?Lys?Val?Ser?Ala?Val?Leu
290 295 300
Glu?Lys?Ala?Gly?Met?Ala?Thr?Ala?Ser?Ser?Ser?Ala?Pro?Glu?Asn?Arg
305 310 315 320
Tyr?Ile?Val?Lys?Leu?Lys?Glu?Gly?Lys?Lys?Pro?Gly?Ser?Phe?Lys?Ser
325 330 335
Lys?Ala?Gln?Ser?Ser?Gly?Val?Gln?Ala?Leu?Glu?Pro?Leu?Gly?Lys?Ser
340 345 350
Lys?Thr?Ala?Phe?Lys?Asp?Met?Tyr?Val?Val?Glu?Met?Lys?Glu?Ser?Arg
355 360 365
Ser?Ser?Gly?Phe?Lys?Ala?Ala?Ala?Lys?Gln?Tyr?Gln?Ala?Ala?Ala?Ser
370 375 380
Lys?Ile?Ala?Lys?Met?Pro?Glu?Val?Glu?Phe?Val?Glu?Gln?Val?Gln?Gln
385 390 395 400
Tyr?Glu?Ala?Leu?Ser?Arg?Asp?Thr?Gln?Tyr?Pro?Tyr?Gln?Trp?Ser?Leu
405 410 415
Lys?Asn?Asn?Gly?Lys?Asn?Arg?Ala?Ala?Asn?Ala?Asp?Ile?Gln?Phe?Glu
420 425 430
Gln?Leu?Gln?Lys?Leu?Met?Lys?Gly?Lys?Lys?Leu?Lys?Asp?Thr?Val?Ile
435 440 445
Ala?Val?Val?Asp?Thr?Gly?Val?Asp?His?Thr?Leu?Ala?Asp?Leu?Ser?Gly
450 455 460
Ser?Val?Lys?Lys?Asp?Glu?Gly?Tyr?Asn?Tyr?Val?Gly?Arg?Thr?Ala?Asp
465 470 475 480
Ala?Met?Asp?Asp?Asn?Gly?His?Gly?Thr?His?Val?Ser?Gly?Ile?Ile?Ala
485 490 495
Ala?Ala?Gln?Asp?Asn?His?Phe?Ser?Met?Ala?Gly?Ile?Asn?Ala?Tyr?Ala
500 505 510
Lys?Ile?Leu?Pro?Val?Lys?Val?Leu?Asp?Ser?Ser?Gly?Ser?Gly?Asp?Thr
515 520 525
Glu?Gln?Ile?Ala?Asn?Gly?Ile?Ile?Tyr?Ala?Ala?Asp?His?Gly?Ala?Lys
530 535 540
Val?Ile?Asn?Leu?Ser?Leu?Gly?Gly?Pro?Tyr?Ser?Arg?Val?Met?Glu?Tyr
545 550 555 560
Ala?Leu?Lys?Tyr?Ala?Ala?Ser?Lys?Asn?Val?Thr?Ile?Val?Ala?Ala?Thr
565 570 575
Gly?Asn?Asp?Gly?Val?Ser?Glu?Ile?Ser?Tyr?Pro?Ala?Ser?Ser?Lys?Tyr
580 585 590
Thr?Leu?Ser?Val?Gly?Ala?Thr?Asn?Asn?Leu?Asp?Leu?Val?Ser?Asp?Tyr
595 600 605
Ser?Asn?Tyr?Gly?Lys?Gly?Leu?Asp?Met?Val?Ala?Pro?Gly?Thr?Asp?Ile
610 615 620
Pro?Ser?Leu?Val?Pro?Asp?Gly?Asn?Val?Thr?Tyr?Met?Ser?Gly?Thr?Ser
625 630 635 640
Met?Ala?Ala?Pro?His?Val?Ala?Ala?Ala?Ala?Gly?Leu?Leu?Leu?Ser?Gln
645 650 655
Asn?Pro?Ser?Leu?Lys?Pro?Lys?Gln?Ile?Ala?Ser?Leu?Leu?Thr?Glu?Thr
660 665 570
Thr?Ala?Asp?Val?Ala?Phe?Glu?Glu?Gln?Asp?Asn?Pro?Asn?Pro?Asp?Tyr
675 680 685
Asp?Leu?Asp?Ile?Glu?Pro?Ala?Ala?Gln?Ile?Pro?Gly?Tyr?Asp?Phe?Val
690 695 700
Ser?Gly?Trp?Gly?Arg?Leu?Asn?Val?Phe?His?Ala?Ala?Ser?Val?Phe?Glu
705 710 715 720
Leu?Asn?Met?Lys?Val?His?Pro?Val?Leu?Asn?Arg?His?Thr?Ala?Val?Thr
725 730 735
Gly?Thr?Ala?Lys?Ser?Gly?Val?Thr?Val?Lys?Ile?Leu?Arg?Gly?Lys?Gln
740 745 750
Val?Leu?Gly?Thr?Gly?Thr?Ala?Gly?Lys?Ser?Gly?Ala?Phe?Ser?Val?Lys
755 760 765
Ile?Pro?Ala?Gln?Lys?Ala?Gly?Gln?Val?Leu?His?Val?Ala?Ala?Ser?Gly
770 775 780
His?Gln?Ala?Glu?Thr?Ser?Leu?Arg?Thr?Val?Val?Glu?Lys?Ala?Pro?Lys
785 790 795 800
Asn?Pro?Ser?Val?Lys?Arg?Ile?Thr?Asn?Lys?Asp?Thr?Ala?Val?Thr?Gly
805 810 815
Arg?Thr?Ala?Ala?Gly?Tyr?Thr?Ile?Lys?Val?Lys?Asn?Ala?Cys?Lys?Lys
820 825 830
Val?Ile?Ala?Gln?Gly?Arg?Ala?Asp?Ala?Ser?Val?Ser?Val?Lys?Val?Lys
835 840 845
Ile?Asn?Lys?Gln?Lys?Asp?Met?Pro?Phe?Cys?Met?Ser?Pro?His?Leu?Leu
850 855 860
Met?Thr?Thr?Glu?Lys?Ala?Ala?Met?Ser?Lys
865 870
<210>55
<211>1540
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(505)..(1050)
<400>55
catcagaaaa?gcactttctt?tttctgcatt?tgaaccggtc?atcgtcagag?ctgcgggttt 60
tatccgtttt?atccgcttta?gctgttttat?ctgttttatt?cgttttatct?gttttattac 120
tatccgccga?acgccgcgct?ttcattgact?gtctttcttt?ttcccgctca?gggctgtatg 180
gatcgtaatt?gtcatagtac?tcttcgtagc?tgctcatttt?gaggcaatca?ccgcctttgg 240
gtcatgatat?gataatgtat?tataaagagg?ggcgttttgc?ctgtgcatat?ataacggttt 300
ttcgtgctgt?aaataagctg?atcaactgac?aaaattcaac?attgaataaa?ggatttttcg 360
tttcaaccgc?taatattata?tgtatcaacc?atttttttac?ggctgcgcct?gccggtgctt 420
gcggaaagca?ttcagacgga?cggcctgata?tgatagaagg?agcggaatgc?cgatgaaacg 480
atccggaagt?gaaggagagt?actt?atg?aaa?aat?agt?aga?aaa?aaa?gag?atc 531
Met?Lys?Asn?Ser?Arg?Lys?Lys?Glu?Ile
1 5
tta?tcg?tgg?gtg?aaa?aca?ctt?gtc?atc?gcc?gca?gcg?ctt?gtg?atg?gtt 579
Leu?Ser?Trp?Val?Lys?Thr?Leu?Val?Ile?Ala?Ala?Ala?Leu?Val?Met?Val
10 15 20 25
tgc?cgc?tat?ttt?ctg?ttt?acg?ccg?tca?acc?gtt?ttg?gga?gat?tca?atg 627
Cys?Arg?Tyr?Phe?Leu?Phe?Thr?Pro?Ser?Thr?Val?Leu?Gly?Asp?Ser?Met
30 35 40
tat?ccg?act?tta?gag?gac?ggc?aac?atg?gtc?atg?gtt?agt?aag?atc?agc 675
Tyr?Pro?Thr?Leu?G1u?Asp?Gly?Asn?Met?Val?Met?Val?Ser?Lys?Ile?Ser
45 50 55
gat?att?cag?cgc?ttt?gat?aaa?att?att?ttt?cac?gcg?cct?gat?tcg?gat 723
Asp?Ile?Gln?Arg?Phe?Asp?Lys?Ile?Ile?Phe?His?Ala?Pro?Asp?Ser?Asp
60 65 70
gaa?aac?tat?gtg?aaa?agg?gtg?atc?ggg?ctc?ccg?gga?gat?acg?atc?gaa 771
G1u?Asn?Tyr?Val?Lys?Arg?Val?Ile?Gly?Leu?Pro?Gly?Asp?Thr?Ile?Glu
75 80 85
atg?aag?gat?gat?gtc?ctt?tat?ata?aac?gga?aaa?gca?tac?gat?gaa?cct 819
Met?Lys?Asp?Asp?Val?Leu?Tyr?Ile?Asn?Gly?Lys?Ala?Tyr?Asp?Glu?Pro
90 95 100 105
tat?tta?aag?aaa?aac?aag?caa?aag?ctg?acg?ctt?cat?gaa?cat?tta?acc 867
Tyr?Leu?Lys?Lys?Asn?Lys?Gln?Lys?Leu?Thr?Leu?His?Glu?His?Leu?Thr
110 115 120
gat?aat?ttc?aca?ttg?gag?aaa?tta?aca?ggt?gaa?caa?aag?gtg?ccg?gaa 915
Asp?Asn?Phe?Thr?Leu?Glu?Lys?Leu?Thr?Gly?Glu?Gln?Lys?Val?Pro?Glu
125 130 135
gat?cac?ttg?ttc?gtg?atg?ggg?gat?aac?cgt?caa?aac?agc?aaa?gac?agc 963
Asp?His?Leu?Phe?Val?Met?Gly?Asp?Asn?Arg?Gln?Asn?Ser?Lys?Asp?Ser
140 145 150
cgc?ttt?ttc?gga?ttt?atc?aca?atg?gac?tcc?gtt?gtc?ggc?aaa?gtt?gag 1011
Arg?Phe?Phe?Gly?Phe?Ile?Thr?Met?Asp?Ser?Val?Val?Gly?Lys?Val?Glu
155 160 165
ttc?cgt?tat?ttc?cct?ttc?aat?gag?atc?ggc?gga?ata?gaa?tagaaaacaa 1060
Phe?Arg?Tyr?Phe?Pro?Phe?Asn?Glu?Ile?Gly?Gly?Ile?Glu
170 175 180
aaaggcttcc?catgatacct?aatgggaagt?ctttttgttt?gtcgcaaagt?caccaaaagt 1120
ggtttaaacc?aagccgctat?gcttgagcac?ggcggcaatt?ctactattca?cttaaattta 1180
aattagagcc?agtttttgat?atcgcagttg?cagaattagg?tctctctgaa?ttataaggat 1240
tgcctttaat?ttggtataaa?agcctgttta?atcgctgatt?acccaaacaa?aaataactaa 1300
aaaatcctcg?atttaacaat?ttagtgtatg?gtactcttta?aacatgtaat?caaaaaacta 1360
tctttttcgc?tccaaagcaa?cataccgtat?ttcttggttt?gttttttagt?tgttctaaat 1420
attttttatt?gttatcgcaa?ttcttacgtt?gtttgctgca?atgattgaag?tactatatta 1480
cataaattct?gataggaggt?cattatgttt?aatagacaac?agatagatca?tctgcttgaa 1540
<210>56
<211>182
<212>PRT
＜213〉Bacillus licheniformis
<400>56
Met?Lys?Asn?Ser?Arg?Lys?Lys?Glu?Ile?Leu?Ser?Trp?Val?Lys?Thr?Leu
1 5 10 15
Val?Ile?Ala?Ala?Ala?Leu?Val?Met?Val?Cys?Arg?Tyr?Phe?Leu?Phe?Thr
20 25 30
Pro?Ser?Thr?Val?Leu?Gly?Asp?Ser?Met?Tyr?Pro?Thr?Leu?Glu?Asp?Gly
35 40 45
Asn?Met?Val?Met?Val?Ser?Lys?Ile?Ser?Asp?Ile?Gln?Arg?Phe?Asp?Lys
50 55 60
Ile?Ile?Phe?His?Ala?Pro?Asp?Ser?Asp?Glu?Asn?Tyr?Val?Lys?Arg?Val
65 70 75 80
Ile?Gly?Leu?Pro?Gly?Asp?Thr?Ile?Glu?Met?Lys?Asp?Asp?Val?Leu?Tyr
85 90 95
Ile?Asn?Gly?Lys?Ala?Tyr?Asp?Glu?Pro?Tyr?Leu?Lys?Lys?Asn?Lys?Gln
100 105 110
Lys?Leu?Thr?Leu?His?Glu?His?Leu?Thr?Asp?Asn?Phe?Thr?Leu?Glu?Lys
115 120 125
Leu?Thr?Gly?Glu?Gln?Lys?Val?Pro?Glu?Asp?His?Leu?Phe?Val?Met?Gly
130 135 140
Asp?Asn?Arg?Gln?Asn?Ser?Lys?Asp?Ser?Arg?Phe?Phe?Gly?Phe?Ile?Thr
145 150 155 160
Met?Asp?Ser?Val?Val?Gly?Lys?Val?Glu?Phe?Arg?Tyr?Phe?Pro?Phe?Asn
165 170 175
Glu?Ile?Gly?Gly?Ile?Glu
180
<210>57
<211>2212
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(2)..(604)
<400>57
g?ttt?tca?ggc?tct?gtt?aca?caa?gga?atc?atc?tca?gga?acc?gaa?cgc?gct 49
Phe?Ser?Gly?Ser?Val?Thr?Gln?Gly?Ile?Ile?Ser?Gly?Thr?Glu?Arg?Ala
1 5 10 15
gtg?ccg?gtc?gat?tcc?aac?gga?gac?gga?cag?ccg?gac?tgg?aac?gcc?gaa 97
Val?Pro?Val?Asp?Ser?Asn?Gly?Asp?Gly?Gln?Pro?Asp?Trp?Asn?Ala?Glu
20 25 30
gta?ctg?cag?acg?gat?gct?gcc?att?aac?ccg?ggt?aac?agc?gga?ggc?ggg 145
Val?Leu?Gln?Thr?Asp?Ala?Ala?Ile?Asn?Pro?Gly?Asn?Ser?Gly?Gly?Gly
35 40 45
ctg?ttt?aat?att?gat?gga?aaa?gtc?atc?ggc?att?aat?tcg?atg?aaa?atc 193
Leu?Phe?Asn?Ile?Asp?Gly?Lys?Val?Ile?Gly?Ile?Asn?Ser?Met?Lys?Ile
50 55 60
gct?gaa?tca?gcc?gtc?gaa?ggc?atc?ggt?ctg?agc?att?ccg?gcc?aac?ctt 241
Ala?Glu?Ser?Ala?Val?Glu?Gly?Ile?Gly?Leu?Ser?Ile?Pro?Ala?Asn?Leu
65 70 75 80
gcc?att?ccg?gtt?atc?gaa?gat?ttg?gaa?acg?tac?ggt?gaa?gtg?aga?cgg 289
Ala?Ile?Pro?Val?Ile?Glu?Asp?Leu?Glu?Thr?Tyr?Gly?Glu?Val?Arg?Arg
85 90 95
ccg?tat?ctc?gga?att?gag?atg?aaa?tca?ctt?ggc?gat?atc?gca?agc?tac 337
Pro?Tyr?Leu?Gly?Ile?Glu?Met?Lys?Ser?Leu?Gly?Asp?Ile?Ala?Ser?Tyr
100 105 110
cac?tgg?cag?gaa?acg?ctt?aaa?ctg?ccg?aaa?aac?gta?aca?tca?ggc?gta 385
His?Trp?Gln?Glu?Thr?Leu?Lys?Leu?Pro?Lys?Asn?Val?Thr?Ser?Gly?Val
115 120 125
gtt?gtg?atg?ggt?gtt?cag?ccg?gta?tct?cct?gca?ggc?aga?gcc?ggt?ctg 433
Val?Val?Met?Gly?Val?Gln?Pro?Val?Ser?Pro?Ala?Gly?Arg?Ala?Gly?Leu
130 135 140
aaa?gag?ctc?gat?gtc?atc?gtc?gaa?ttt?aac?ggt?gac?cgt?gtt?tac?gat 481
Lys?Glu?Leu?Asp?Val?Ile?Val?Glu?Phe?Asn?Gly?Asp?Arg?Val?Tyr?Asp
145 150 155 160
atc?gtt?gat?ctg?cgt?aaa?aag?ctt?tac?acc?aaa?aac?gtc?ggc?gac?aag 529
Ile?Val?Asp?Leu?Arg?Lys?Lys?Leu?Tyr?Thr?Lys?Asn?Val?Gly?Asp?Lys
165 170 175
gta?aaa?atc?aaa?tac?ctg?cgg?ggc?gga?aaa?gaa?aag?aca?aca?gaa?gta 577
Val?Lys?Ile?Lys?Tyr?Leu?Arg?Gly?Gly?Lys?Glu?Lys?Thr?Thr?Glu?Val
180 185 190
aag?ctg?acc?cga?tcc?caa?tta?gga?agc?tgataaagac?agtctggccg 624
Lys?Leu?Thr?Arg?Ser?Gln?Leu?Gly?Ser
195 200
gtacggccgg?gctgtttttt?tattatgaag?cgcccaacga?atgatcgtca?aaaggaaaaa 684
atgtgatttt?tggagagtga?aatgagattt?tttctgagtg?aataatcgaa?tttttgggtg 744
tgaattccaa?gtttttgccc?tgcaaaaatt?atagtttgta?aaaaatggac?aaagcatcat 804
attgcgtttc?aattttgcgt?acgatatgat?gactttaata?ttctgacaaa?tggccgattt 864
aatgcaggat?ttaaagatca?gggcggaaag?ggagatagga?tgagtcagca?tgcacttgta 924
ttacaatctg?attttggcct?tgatgacggg?gcggtaaatg?cgatgtacgg?tgtggcttat 984
tcggttgaca?gttcaattcg?gatttttgat?ttgacgcaca?acattcccgt?ttttcacatt 1044
tgtgaagctt?cctatcggct?gctgcagtcc?gtttcttatt?ggcctgaagg?cactgtcttc 1104
gtgtcggtcg?tcgatccagg?ggtcggctca?gaacgccgca?gcgtcgcagt?cagaacctct 1164
tccaatcaat?atatcatcac?accagacaac?ggaacactca?cccacatcag?ccggcaaaat 1224
ggaattgccg?aggcgcgcta?tcttgatgaa?gcgcaaaaca?ggcttccaaa?atcaggcgaa 1284
tcacatacat?tccatggacg?cgacatttac?gcctacaccg?gggcaagact?ggcagccggc 1344
gctctcagct?tcgacaggat?cggtccggaa?gtttctatag?attccatcgt?caaactgccg 1404
gtcatggaag?cgtacatcga?aaatgattgg?ataaccggaa?cgatcgatat?tttagatgtc 1464
cggtttggaa?acctttggac?gaatatcagc?cggacgctct?tcaagtcact?tgatgttcaa 1524
tacggagatc?cagtagaagt?cgtcatcaag?catgaagaca?caaaagtcta?tcagcaaacg 1584
ttggcatttg?gccggtcgtt?tgccgatatg?cctgtaggca?agccggttgt?ttatatcaat 1644
tcattagacc?gcttgggcat?tgccctcaac?caaggatcat?ttgctaaaac?ataccatatt 1704
ggcacaggca?tcaactggcg?gatttctctt?cgaaaaagta?tatagcgtta?gacaaaagag 1764
cctgattttc?cagtcaggct?cttgcttttg?tgtcattctt?atgacataaa?aacggttgtc 1824
acacattttt?cagctaatcg?tgaagacagc?aacctcttca?aaaaatggtt?cttttgtcac 1884
attggtacct?aagtcatatt?gtctaaaaca?agagaaaaga?atatattgag?ggggtgtgcc 1944
gctgctatga?aaaaattcat?tcaattcatt?tgtttggaaa?acgagtgtcc?ggaggaatga 2004
tatgaacgac?aatgtgcagg?ctgcgttgaa?aacgctgtct?aatgtaaaga?gcagaagtat 2064
cttgtccgtt?tttgtgctat?gtctgatcac?actgggggtt?tacataccat?actggtttct 2124
atccaggaaa?aaaagcttcg?atttgctgcc?ttatcatgat?attccctaca?ccacgttaaa 2184
agctatcata?ttttatcatt?tattttct 2212
<210>58
<211>201
<212>PRT
＜213〉Bacillus licheniformis
<400>58
Phe?Ser?Gly?Ser?Val?Thr?Gln?Gly?Ile?Ile?Ser?Gly?Thr?Glu?Arg?Ala
1 5 10 15
Val?Pro?Val?Asp?Ser?Asn?Gly?Asp?Gly?Gln?Pro?Asp?Trp?Asn?Ala?Glu
20 25 30
Val?Leu?Gln?Thr?Asp?Ala?Ala?Ile?Asn?Pro?Gly?Asn?Ser?Gly?Gly?Gly
35 40 45
Leu?Phe?Asn?Ile?Asp?Gly?Lys?Val?Ile?Gly?Ile?Asn?Ser?Met?Lys?Ile
50 55 60
Ala?Glu?Ser?Ala?Val?Glu?Gly?Ile?Gly?Leu?Ser?Ile?Pro?Ala?Asn?Leu
65 70 75 80
Ala?Ile?Pro?Val?Ile?Glu?Asp?Leu?Glu?Thr?Tyr?Gly?Glu?Val?Arg?Arg
85 90 95
Pro?Tyr?Leu?Gly?Ile?Glu?Met?Lys?Ser?Leu?Gly?Asp?Ile?Ala?Ser?Tyr
100 105 110
His?Trp?Gln?Glu?Thr?Leu?Lys?Leu?Pro?Lys?Asn?Val?Thr?Ser?Gly?Val
115 120 125
Val?Val?Met?Gly?Val?Gln?Pro?Val?Ser?Pro?Ala?Gly?Arg?Ala?Gly?Leu
130 135 140
Lys?Glu?Leu?Asp?Val?Ile?Val?Glu?Phe?Asn?Gly?Asp?Arg?Val?Tyr?Asp
145 150 155 160
Ile?Val?Asp?Leu?Arg?Lys?Lys?Leu?Tyr?Thr?Lys?Asn?Val?Gly?Asp?Lys
165 170 175
Val?Lys?Ile?Lys?Tyr?Leu?Arg?Gly?Gly?Lys?Glu?Lys?Thr?Thr?Glu?Val
180 185 190
Lys?Leu?Thr?Arg?Ser?Gln?Leu?Gly?Ser
195 200
<210>59
<211>1519
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1016)
<400>59
aaaaagctcg?aaaggggttt?atgatgaagg?atattctgcg?tgaaattgga?atgattgccc 60
gagcattgga?ttctataagc?aatatcgaat?ttaaagaaca?tgacctgaca?aaagggcaat 120
atttgtacct?tgtccgaata?tgcgaaaacc?cgggaatcat?tcaggagaag?ctggctgaga 180
tgattaaagt?agacaggacc?accgcagccc?gtgcaataaa?aaaacttgaa?atgaagggct 240
ttattgaacg?gagagatgat?gaacataaca?aaaaaataaa?aaaacttttt?ccgacggaaa 300
agggaaatga?ggtatttcct?tttataaaaa?gagaaaatga?ccattcgaat?cgtgtggcat 360
tagagggatt?ttccgagggg?gaagcggaga?tcatcttcaa?tcttcttcaa?agagtaagaa 420
aaaatgtaga?aaaggactgg?gaattcgtga?aaaaggggaa?caagagaaat?tattgacacg 480
taaaggagcg?gcagattgaa?atg?aca?gta?aat?ata?aaa?agg?tgt?acc?ctt?gaa 533
Met?Thr?Val?Asn?Ile?Lys?Arg?Cys?Thr?Leu?Glu
1 5 10
gac?tta?cac?aag?ctt?cag?gaa?att?ggt?tat?gaa?aca?ttt?aat?gag?aca 581
Asp?Leu?His?Lys?Leu?Gln?Glu?Ile?Gly?Tyr?Glu?Thr?Phe?Asn?Glu?Thr
15 20 25
ttt?aag?cat?cag?aat?tcg?cca?gaa?aat?atg?aaa?gcc?tac?ttg?gac?aag 629
Phe?Lys?His?Gln?Asn?Ser?Pro?Glu?Asn?Met?Lys?Ala?Tyr?Leu?Asp?Lys
30 35 40
gca?ttt?aac?tta?aaa?caa?tta?gaa?aaa?gaa?tta?tcc?aat?agc?tct?tcg 677
Ala?Phe?Asn?Leu?Lys?Gln?Leu?Glu?Lys?Glu?Leu?Ser?Asn?Ser?Ser?Ser
45 50 55
caa?ttc?ttt?ttt?gtt?tat?ttt?aac?aac?gaa?atc?gct?gga?tat?tta?aag 725
Gln?Phe?Phe?Phe?Val?Tyr?Phe?Asn?Asn?Glu?Ile?Ala?Gly?Tyr?Leu?Lys
60 65 70 75
gtc?aac?acc?gat?gag?gcc?cag?tct?gaa?aaa?atg?ggt?gat?gaa?tca?ctt 773
Val?Asn?Thr?Asp?Glu?Ala?Gln?Ser?Glu?Lys?Met?Gly?Asp?Glu?Ser?Leu
80 85 90
gaa?atc?gag?aga?att?tat?atc?aag?aac?aac?ttt?caa?aaa?cac?ggc?ctg 821
Glu?Ile?Glu?Arg?Ile?Tyr?Ile?Lys?Asn?Asn?Phe?Gln?Lys?His?Gly?Leu
95 100 105
ggc?aaa?tat?ctg?ttc?aat?aaa?gct?gtg?gaa?att?gca?aag?gaa?ctg?aat 869
Gly?Lys?Tyr?Leu?Phe?Asn?Lys?Ala?Val?Glu?Ile?Ala?Lys?Glu?Leu?Asn
110 115 120
aaa?aag?aaa?atc?tgg?ctg?ggc?gta?tgg?gaa?aaa?aac?gaa?aat?gcg?att 917
Lys?Lys?Lys?Ile?Trp?Leu?Gly?Val?Trp?Glu?Lys?Asn?Glu?Asn?Ala?Ile
125 130 135
gct?ttt?tat?aag?aaa?atg?ggg?ttt?gta?caa?acc?ggc?gcc?cac?tct?ttt 965
Ala?Phe?Tyr?Lys?Lys?Met?Gly?Phe?Val?Gln?Thr?Gly?Ala?His?Ser?Phe
140 145 150 155
tat?atg?gga?gat?gaa?gag?caa?acg?gac?ttt?atc?atg?gcc?aaa?aca?atc 1013
Tyr?Met?Gly?Asp?Glu?Glu?Gln?Thr?Asp?Phe?Ile?Met?Ala?Lys?Thr?Ile
160 165 170
cta?taacttttta?aaaggaggat?gacgatgtat?attccaaaat?attttaaagt 1066
Leu
cgaaaatgct?gatgaaattt?tggattttgt?ccaaaaaaac?tcttttggca?cgattgttac 1126
gacagaacaa?gggaagccaa?tcgcgactca?tttgccttta?gggctcaata?aaaaaggcga 1186
tgattactat?atcactggcc?atatcgctta?tggaaacccg?caggtgggaa?ctttggaaac 1246
ctgtaaagat?gtgcttgtca?tgtttcaggg?gccgcacgca?tatatttctt?cttcctggta 1306
tgggcatgag?gatgttccaa?catggaatta?tcaagccgtc?catatatacg?gtaaagcaag 1366
cattctagag?agagatgaat?taatagaaga?attaacaatc?atgatggaaa?aatacgagaa 1426
gcatcgggaa?aatccaattt?tatgggataa?cctttctcct?caacttttgg?agagacaact 1486
gaaagcgatt?gtcgggttta?agattaaggt?gga 1519
<210>60
<211>172
<212>PRT
＜213〉Bacillus licheniformis
<400>60
Met?Thr?Val?Asn?Ile?Lys?Arg?Cys?Thr?Leu?Glu?Asp?Leu?His?Lys?Leu
1 5 10 15
Gln?Glu?Ile?Gly?Tyr?Glu?Thr?Phe?Asn?Glu?Thr?Phe?Lys?His?Gln?Asn
20 25 30
Ser?Pro?Glu?Asn?Met?Lys?Ala?Tyr?Leu?Asp?Lys?Ala?Phe?Asn?Leu?Lys
35 40 45
Gln?Leu?Glu?Lys?Glu?Leu?Ser?Asn?Ser?Ser?Ser?Gln?Phe?Phe?Phe?Val
50 55 60
Tyr?Phe?Asn?Asn?Glu?Ile?Ala?Gly?Tyr?Leu?Lys?Val?Asn?Thr?Asp?Glu
65 70 75 80
Ala?Gln?Ser?Glu?Lys?Met?Gly?Asp?Glu?Ser?Leu?Glu?Ile?Glu?Arg?Ile
85 90 95
Tyr?Ile?Lys?Asn?Asn?Phe?Gln?Lys?His?Gly?Leu?Gly?Lys?Tyr?Leu?Phe
100 105 110
Asn?Lys?Ala?Val?Glu?Ile?Ala?Lys?Glu?Leu?Asn?Lys?Lys?Lys?Ile?Trp
115 120 125
Leu?Gly?Val?Trp?Glu?Lys?Asn?Glu?Asn?Ala?Ile?Ala?Phe?Tyr?Lys?Lys
130 135 140
Met?Gly?Phe?Val?Gln?Thr?Gly?Ala?His?Ser?Phe?Tyr?Met?Gly?Asp?Glu
145 150 155 160
Glu?Gln?Thr?Asp?Phe?Ile?Met?Ala?Lys?Thr?Ile?Leu
165 170
<210>61
<211>2072
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(2009)
<400>61
tcgttgacaa?agcggttaaa?atcgcggaca?agccggttga?tcactgttgc?ttgttctccg 60
gcatgtcttc?tcactccggt?tgcatcaaac?atcgtaataa?cggcaaatat?tgcggaaacg 120
gcgaacaaag?aagaatccat?cccgtgatca?agagctacac?cggtcgagag?cgccgtgacg 180
gccgctgagt?gtgaactcgg?cattccgccc?gtgcttgtga?caagccgcca?atcccatttt 240
tttgagacga?caaagtaaat?gggaaccttg?ataaactgag?cgaaaaagat?cgcggcaaag 300
ctcgccagca?aaggaaagtt?tgtgaggatt?tccattttgc?agaacatcct?ttctataaag 360
aaaatagaga?tgaattcaga?aaagcacgca?gttatttgct?aaaatatatg?atatgaaacc 420
ccatcgggaa?aaaatgtttt?tcctattata?acattgttgt?tttaatttta?ctcactttgc 480
tgataaggag?ctgtgaacgc?atg?ttt?tat?gcc?ttt?aaa?gat?ttc?gaa?aaa?aaa 533
Met?Phe?Tyr?Ala?Phe?Lys?Asp?Phe?Glu?Lys?Lys
1 5 10
gaa?acg?ctt?tta?atc?gga?ctg?ttt?aaa?aaa?agc?cgg?ctg?tac?ggt?aag 581
Glu?Thr?Leu?Leu?Ile?Gly?Leu?Phe?Lys?Lys?Ser?Arg?Leu?Tyr?Gly?Lys
15 20 25
gct?gaa?gaa?atc?gac?cgc?ctt?ttg?aac?ggc?cag?ctt?tct?cag?ctg?ctg 629
Ala?Glu?Glu?Ile?Asp?Arg?Leu?Leu?Asn?Gly?Gln?Leu?Ser?Gln?Leu?Leu
30 35 40
aaa?gac?ggg?gat?gta?tct?tcc?aaa?aaa?gcg?aaa?gtg?tct?aaa?ata?ttt 677
Lys?Asp?Gly?Asp?Val?Ser?Ser?Lys?Lys?Ala?Lys?Val?Ser?Lys?Ile?Phe
45 50 55
acc?cct?tcg?ctt?cag?gga?gtc?aaa?cgc?att?tat?atc?gtc?gga?ttg?ggc 725
Thr?Pro?Ser?Leu?Gln?Gly?Val?Lys?Arg?Ile?Tyr?Ile?Val?Gly?Leu?Gly
60 65 70 75
cgt?gaa?gcg?gaa?ttt?acc?ttc?gag?gat?gcg?aag?cag?tgc?ttt?gcc?gaa 773
Arg?Glu?Ala?Glu?Phe?Thr?Phe?Glu?Asp?Ala?Lys?Gln?Cys?Phe?Ala?Glu
80 85 90
gct?gtt?cag?ctg?att?cac?aaa?gat?cga?aag?cag?gag?tta?acc?gtc?atg 821
Ala?Val?Gln?Leu?Ile?His?Lys?Asp?Arg?Lys?Gln?Glu?Leu?Thr?Val?Met
95 100 105
ctc?gac?agc?ttc?gta?tcc?gaa?gaa?gtc?cca?gct?gct?gac?gcc?gca?cat 869
Leu?Asp?Ser?Phe?Val?Ser?Glu?Glu?Val?Pro?Ala?Ala?Asp?Ala?Ala?His
110 115 120
gca?ttg?gct?gaa?tcg?tgc?atg?ctg?tca?tgc?tat?gaa?gtg?cag?gat?tac 917
Ala?Leu?Ala?Glu?Ser?Cys?Met?Leu?Ser?Cys?Tyr?Glu?Val?Gln?Asp?Tyr
125 130 135
aag?cac?aga?tca?aat?gtg?cct?gat?caa?tgt?ctg?caa?agc?gtc?tat?gtt 965
Lys?His?Arg?Ser?Asn?Val?Pro?Asp?Gln?Cys?Leu?Gln?Ser?Val?Tyr?Val
140 145 150 155
ttg?aca?gac?cat?gac?ctg?aag?gag?att?cag?gcg?agt?ctg?cat?gtc?gga 1013
Leu?Thr?Asp?His?Asp?Leu?Lys?Glu?Ile?Gln?Ala?Ser?Leu?His?Val?Gly
160 165 170
cag?gtg?tac?gga?aat?gcg?acg?aat?tcg?gcg?aga?acc?ctc?gtg?aac?atg 1061
Gln?Val?Tyr?Gly?Asn?Ala?Thr?Asn?Ser?Ala?Arg?Thr?Leu?Val?Asn?Met
175 180 185
ccg?ggg?aac?atg?ctg?aca?gcg?gcg?gat?ctc?gcg?tca?tat?gcc?gct?gaa 1109
Pro?Gly?Asn?Met?Leu?Thr?Ala?Ala?Asp?Leu?Ala?Ser?Tyr?Ala?Ala?Glu
190 195 200
ttg?gcg?gcg?aaa?tac?gaa?ttt?gaa?tgt?gaa?att?ctt?gaa?aag?gct?gaa 1157
Leu?Ala?Ala?Lys?Tyr?Glu?Phe?Glu?Cys?Glu?Ile?Leu?Glu?Lys?Ala?Glu
205 210 215
atg?gaa?gag?ctc?ggg?atg?ggc?ggt?ctc?ctc?gcg?gtc?aac?caa?gga?tca 1205
Met?Glu?Glu?Leu?Gly?Met?Gly?Gly?Leu?Leu?Ala?Val?Asn?Gln?Gly?Ser
220 225 230 235
gaa?gag?ccg?ccg?aaa?atg?atc?gtc?ttg?aaa?tat?caa?gga?aaa?gaa?aca 1253
Glu?Glu?Pro?Pro?Lys?Met?Ile?Val?Leu?Lys?Tyr?Gln?Gly?Lys?Glu?Thr
240 245 250
tgg?gat?gac?gtc?atc?ggt?tta?gtc?ggc?aaa?ggg?att?acg?ttt?gat?acc 1301
Trp?Asp?Asp?Val?Ile?Gly?Leu?Val?Gly?Lys?Gly?Ile?Thr?Phe?Asp?Thr
255 260 265
gga?ggc?tat?tcg?att?aag?aca?aag?agc?ggg?att?gtc?ggc?atg?aag?tcc 1349
Gly?Gly?Tyr?Ser?Ile?Lys?Thr?Lys?Ser?Gly?Ile?Val?Gly?Met?Lys?Ser
270 275 280
gat?atg?ggc?gga?gcc?gcc?agc?gtt?ttg?gga?gcg?atg?gaa?gcg?atc?ggc 1397
Asp?Met?Gly?Gly?Ala?Ala?Ser?Val?Leu?Gly?Ala?Met?Glu?Ala?Ile?Gly
285 290 295
gaa?ttg?agg?ccg?gaa?caa?aac?gtg?ctt?gcc?gtg?att?ccg?tcg?act?gat 1445
Glu?Leu?Arg?Pro?Glu?Gln?Asn?Val?Leu?Ala?Val?Ile?Pro?Ser?Thr?Asp
300 305 310 315
aac?atg?att?tcg?gga?agc?gca?atg?aag?ccg?gac?gac?gtc?atc?gtt?tcc 1493
Asn?Met?Ile?Ser?Gly?Ser?Ala?Met?Lys?Pro?Asp?Asp?Val?Ile?Val?Ser
320 325 330
ttg?agc?ggc?aag?acg?att?gag?atc?ttg?aat?acg?gac?gcc?gaa?gga?agg 1541
Leu?Ser?Gly?Lys?Thr?Ile?Glu?Ile?Leu?Asn?Thr?Asp?Ala?Glu?Gly?Arg
335 340 345
ctg?gcg?ctc?gct?gac?gga?ctg?aca?tat?gcg?aag?cac?cac?gga?gct?tcc 1589
Leu?Ala?Leu?Ala?Asp?Gly?Leu?Thr?Tyr?Ala?Lys?His?His?Gly?Ala?Ser
350 355 360
gtg?ctg?atc?gat?gtc?gcg?aca?ctg?acc?gga?ggc?gtc?gtc?gta?gct?ctg 1637
Val?Leu?Ile?Asp?Val?Ala?Thr?Leu?Thr?Gly?Gly?Val?Val?Val?Ala?Leu
365 370 375
gga?acg?gaa?acg?acg?gga?gcg?atg?aca?aat?cat?gat?ccg?ctt?tat?cag 1685
Gly?Thr?Glu?Thr?Thr?Gly?Ala?Met?Thr?Asn?His?Asp?Pro?Leu?Tyr?Gln
380 385 390 395
cag?gtg?aga?cag?gcg?gct?gaa?gaa?gcg?gga?gaa?gcg?att?tgg?cag?ctt 1733
Gln?Val?Arg?Gln?Ala?Ala?Glu?Glu?Ala?Gly?Glu?Ala?Ile?Trp?Gln?Leu
400 405 410
ccg?att?act?gaa?aaa?gac?aaa?aaa?aga?gta?aaa?aac?agc?caa?atg?gcc 1781
Pro?Ile?Thr?Glu?Lys?Asp?Lys?Lys?Arg?Val?Lys?Asn?Ser?Gln?Met?Ala
415 420 425
gat?ctt?aac?aat?tca?ccg?ggc?agg?gaa?ggc?cat?gcg?att?atg?gcc?gga 1829
Asp?Leu?Asn?Asn?Ser?Pro?Gly?Arg?Glu?Gly?His?Ala?Ile?Met?Ala?Gly
430 435 440
acg?ttc?ctc?ggc?gaa?ttc?gcc?gag?cag?acg?cca?tgg?gtt?cac?ctt?gac 1877
Thr?Phe?Leu?Gly?Glu?Phe?Ala?Glu?Gln?Thr?Pro?Trp?Val?His?Leu?Asp
445 450 455
atc?gcc?gga?acg?gca?acg?aca?gct?caa?aac?tca?tgc?ttt?gga?ccg?aaa 1925
Ile?Ala?Gly?Thr?Ala?Thr?Thr?Ala?Gln?Asn?Ser?Cys?Phe?Gly?Pro?Lys
460 465 470 475
ggc?gga?acg?ggc?gtg?atg?gtg?aga?acg?ctt?gtc?acg?ttt?gtc?gag?cgg 1973
Gly?Gly?Thr?Gly?Val?Met?Val?Arg?Thr?Leu?Val?Thr?Phe?Val?Glu?Arg
480 485 490
gtt?tcg?gga?aat?ttt?gta?gtc?ttt?cat?tcc?ctt?tgc?tagaggggga 2019
Val?Ser?Gly?Asn?Phe?Val?Val?Phe?His?Ser?Leu?Cys
495 500
tttttttatg?gaaaaacgca?gatgtctcat?tgacgcatta?taaacatggt?gta 2072
<210>62
<211>503
<212>PRT
＜213〉Bacillus licheniformis
<400>62
Met?Phe?Tyr?Ala?Phe?Lys?Asp?Phe?Glu?Lys?Lys?Glu?Thr?Leu?Leu?Ile
1 5 10 15
Gly?Leu?Phe?Lys?Lys?Ser?Arg?Leu?Tyr?Gly?Lys?Ala?Glu?Glu?Ile?Asp
20 25 30
Arg?Leu?Leu?Asn?Gly?Gln?Leu?Ser?Gln?Leu?Leu?Lys?Asp?Gly?Asp?Val
35 40 45
Ser?Ser?Lys?Lys?Ala?Lys?Val?Ser?Lys?Ile?Phe?Thr?Pro?Ser?Leu?Gln
50 55 60
Gly?Val?Lys?Arg?Ile?Tyr?Ile?Val?Gly?Leu?Gly?Arg?Glu?Ala?Glu?Phe
65 70 75 80
Thr?Phe?Glu?Asp?Ala?Lys?Gln?Cys?Phe?Ala?Glu?Ala?Val?Gln?Leu?Ile
85 90 95
His?Lys?Asp?Arg?Lys?Gln?Glu?Leu?Thr?Val?Met?Leu?Asp?Ser?Phe?Val
100 105 110
Ser?Glu?Glu?Val?Pro?Ala?Ala?Asp?Ala?Ala?His?Ala?Leu?Ala?Glu?Ser
115 120 125
Cys?Met?Leu?Ser?Cys?Tyr?Glu?Val?Gln?Asp?Tyr?Lys?His?Arg?Ser?Asn
130 135 140
Val?Pro?Asp?Gln?Cys?Leu?Gln?Ser?Val?Tyr?Val?Leu?Thr?Asp?His?Asp
145 150 155 160
Leu?Lys?Glu?Ile?Gln?Ala?Ser?Leu?His?Val?Gly?Gln?Val?Tyr?Gly?Asn
165 170 175
Ala?Thr?Asn?Ser?Ala?Arg?Thr?Leu?Val?Asn?Met?Pro?Gly?Asn?Met?Leu
180 185 190
Thr?Ala?Ala?Asp?Leu?Ala?Ser?Tyr?Ala?Ala?Glu?Leu?Ala?Ala?Lys?Tyr
195 200 205
Glu?Phe?Glu?Cys?Glu?Ile?Leu?Glu?Lys?Ala?Glu?Met?Glu?Glu?Leu?Gly
210 215 220
Met?Gly?Gly?Leu?Leu?Ala?Val?Asn?Gln?Gly?Ser?Glu?Glu?Pro?Pro?Lys
225 230 235 240
Met?Ile?Val?Leu?Lys?Tyr?Gln?Gly?Lys?Glu?Thr?Trp?Asp?Asp?Val?Ile
245 250 255
Gly?Leu?Val?Gly?Lys?Gly?Ile?Thr?Phe?Asp?Thr?Gly?Gly?Tyr?Ser?Ile
260 265 270
Lys?Thr?Lys?Ser?Gly?Ile?Val?Gly?Met?Lys?Ser?Asp?Met?Gly?Gly?Ala
275 280 285
Ala?Ser?Val?Leu?Gly?Ala?Met?Glu?Ala?Ile?Gly?Glu?Leu?Arg?Pro?Glu
290 295 300
Gln?Asn?Val?Leu?Ala?Val?Ile?Pro?Ser?Thr?Asp?Asn?Met?Ile?Ser?Gly
305 310 315 320
Ser?Ala?Met?Lys?Pro?Asp?Asp?Val?Ile?Val?Ser?Leu?Ser?Gly?Lys?Thr
325 330 335
Ile?Glu?Ile?Leu?Asn?Thr?Asp?Ala?Glu?Gly?Arg?Leu?Ala?Leu?Ala?Asp
340 345 350
Gly?Leu?Thr?Tyr?Ala?Lys?His?His?Gly?Ala?Ser?Val?Leu?Ile?Asp?Val
355 360 365
Ala?Thr?Leu?Thr?Gly?Gly?Val?Val?Val?Ala?Leu?Gly?Thr?Glu?Thr?Thr
370 375 380
Gly?Ala?Met?Thr?Asn?His?Asp?Pro?Leu?Tyr?Gln?Gln?Val?Arg?Gln?Ala
385 390 395 400
Ala?Glu?Glu?Ala?Gly?Glu?Ala?Ile?Trp?Gln?Leu?Pro?Ile?Thr?Glu?Lys
405 410 415
Asp?Lys?Lys?Arg?Val?Lys?Asn?Ser?Gln?Met?Ala?Asp?Leu?Asn?Asn?Ser
420 425 430
Pro?Gly?Arg?Glu?Gly?His?Ala?Ile?Met?Ala?Gly?Thr?Phe?Leu?Gly?Glu
435 440 445
Phe?Ala?Glu?Gln?Thr?Pro?Trp?Val?His?Leu?Asp?Ile?Ala?Gly?Thr?Ala
450 455 460
Thr?Thr?Ala?Gln?Asn?Ser?Cys?Phe?Gly?Pro?Lys?Gly?Gly?Thr?Gly?Val
65 470 475 480
Met?Val?Arg?Thr?Leu?Val?Thr?Phe?Val?Glu?Arg?Val?Ser?Gly?Asn?Phe
485 490 495
Val?Val?Phe?His?Ser?Leu?Cys
500
<210>63
<211>1544
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1043)
<400>63
ctacgcgaca?ggcatgcggg?taagcgaact?ttgcagcctg?aaggaatctg?atttggatct 60
gtttttagat?acggttttgg?tccacgggaa?aggcaggaag?caaaggtata?ttccatttgg 120
ttcattcgcc?cgggaggctt?tggatctgta?tttgcaaaat?ggaagacgca?tccttctgtt 180
aaaagcgaaa?gagccctgcc?cttttatttt?tttgaatcaa?aggggcgggc?ccttaacccc 240
gaggggggtt?cgttacattt?tgggagaact?ggtcaagaag?acttcgggta?cattacatat 300
acatccgcat?atgctccgcc?atacatttgc?cacccatttg?ttaaatgagg?gtgcggatct 360
gaggagcgtt?caggaactgc?ttgggcattc?aaatctttct?tcaacccagg?tgtatacgca 420
tgtatctaaa?gatatgctca?gaaagacata?tatgtctcat?caccctcggg?cacataaagg 480
gaaataaagg?aggttctttt?atg?tca?tct?ttt?cac?gca?acg?acg?ata?ttt?gcc 533
Met?Ser?Ser?Phe?His?Ala?Thr?Thr?Ile?Phe?Ala
1 5 10
gtc?cag?cac?aac?gga?aaa?agc?gcg?atg?gcg?gga?gac?ggc?cag?gtt?acg 581
Val?Gln?His?Asn?Gly?Lys?Ser?Ala?Met?Ala?Gly?Asp?Gly?Gln?Val?Thr
15 20 25
ttc?ggt?cag?gct?gtc?gtc?atg?aag?cat?acc?gca?aga?aag?gta?aga?aaa 629
Phe?Gly?Gln?Ala?Val?Val?Met?Lys?His?Thr?Ala?Arg?Lys?Val?Arg?Lys
30 35 40
ctc?ttc?aac?gga?aag?gtc?att?gcc?ggt?ttt?gcc?ggt?tct?gtt?gca?gac 677
Leu?Phe?Asn?Gly?Lys?Val?Ile?Ala?Gly?Phe?Ala?Gly?Ser?Val?Ala?Asp
45 50 55
gct?ttc?acg?ctc?ttt?gaa?atg?ttt?gaa?gcg?aag?ctt?gaa?gaa?tac?aac 725
Ala?Phe?Thr?Leu?Phe?Glu?Met?Phe?Glu?Ala?Lys?Leu?Glu?Glu?Tyr?Asn
60 65 70 75
ggc?aat?ttg?cag?cgg?gcg?gca?gtc?gag?ctt?gca?aaa?gag?tgg?cga?agc 773
Gly?Asn?Leu?Gln?Arg?Ala?Ala?Val?Glu?Leu?Ala?Lys?Glu?Trp?Arg?Ser
80 85 90
gat?aaa?gtc?ctc?aga?aag?ctt?gag?gcg?atg?ctg?atc?gtc?atg?aat?gcc 821
Asp?Lys?Val?Leu?Arg?Lys?Leu?Glu?Ala?Met?Leu?Ile?Val?Met?Asn?Ala
95 100 105
gac?agc?atg?ctg?ctc?gtt?tcc?gga?aca?ggc?gaa?gtg?att?gaa?ccg?gac 869
Asp?Ser?Met?Leu?Leu?Val?Ser?Gly?Thr?Gly?Glu?Val?Ile?Glu?Pro?Asp
110 115 120
gac?ggc?att?ttg?gcg?atc?ggc?tca?ggt?gga?aac?tac?gcg?ctt?gca?gcc 917
Asp?Gly?Ile?Leu?Ala?Ile?Gly?Ser?Gly?Gly?Asn?Tyr?Ala?Leu?Ala?Ala
125 130 135
gga?agg?gcg?ctg?aaa?cgt?cat?gcc?ggc?agc?cag?ctt?gac?gcg?aaa?gcg 965
Gly?Arg?Ala?Leu?Lys?Arg?His?Ala?Gly?Ser?Gln?Leu?Asp?Ala?Lys?Ala
140 145 150 155
att?gcg?agg?gct?tca?ctt?gaa?acc?gcg?ggc?gaa?atc?tgt?gtg?tat?acg 1013
Ile?Ala?Arg?Ala?Ser?Leu?Glu?Thr?Ala?Gly?Glu?Ile?Cys?Val?Tyr?Thr
160 165 170
aac?gat?cag?atc?att?gta?gaa?gaa?ctt?gaa?tagaaaggat?atgaggcata 1063
Asn?Asp?Gln?Ile?Ile?Val?Glu?Glu?Leu?Glu
175 180
tggaaaaaaa?gccgctgact?cctaggcaga?tcgtcgaaag?gcttgatcaa?tacatcgtcg 1123
gccagctcga?tgcgaaaaaa?gcggtcgccg?tggcattgag?gaaccgctac?agaagaagtc 1183
ttcttgatga?aaaactgcgc?gaagaaatcg?ttccgaaaaa?catattgatg?atgggcccga 1243
caggtgtggg?aaaaaccgag?attgcaagac?gaatcgcgaa?actcgtcggc?gctccgttcg 1303
tcaaaatcga?agcgacgaag?ttcacggaag?tcggttatgt?gggcagagat?gtggagtcga 1363
tggtcaggga?tcttgtggaa?acgtcagtcc?gcctcgtcaa?agaggaaaaa?atgaacgaag 1423
tgaaaggcat?cgctgaagaa?aacgcaaata?aacgcctcgt?ccgcctgctt?gtaccgggaa 1483
gaaaaaaaca?gacaggtgcg?aaaaatcctt?ttgaaatgct?gtttggcgga?aatcaggatc 1543
a 1544
<210>64
<211>181
<212>PRT
＜213〉Bacillus licheniformis
<400>64
Met?Ser?Ser?Phe?His?Ala?Thr?Thr?Ile?Phe?Ala?Val?Gln?His?Asn?Gly
1 5 10 15
Lys?Ser?Ala?Met?Ala?Gly?Asp?Gly?Gln?Val?Thr?Phe?Gly?Gln?Ala?Val
20 25 30
Val?Met?Lys?His?Thr?Ala?Arg?Lys?Val?Arg?Lys?Leu?Phe?Asn?Gly?Lys
35 40 45
Val?Ile?Ala?Gly?Phe?Ala?Gly?Ser?Val?Ala?Asp?Ala?Phe?Thr?Leu?Phe
50 55 60
Glu?Met?Phe?Glu?Ala?Lys?Leu?Glu?Glu?Tyr?Asn?Gly?Asn?Leu?Gln?Arg
65 70 75 80
Ala?Ala?Val?Glu?Leu?Ala?Lys?Glu?Trp?Arg?Ser?Asp?Lys?Val?Leu?Arg
85 90 95
Lys?Leu?Glu?Ala?Met?Leu?Ile?Val?Met?Asn?Ala?Asp?Ser?Met?Leu?Leu
100 105 110
Val?Ser?Gly?Thr?Gly?Glu?Val?Ile?Glu?Pro?Asp?Asp?Gly?Ile?Leu?Ala
115 120 125
Ile?Gly?Ser?Gly?Gly?Asn?Tyr?Ala?Leu?Ala?Ala?Gly?Arg?Ala?Leu?Lys
130 135 140
Arg?His?Ala?Gly?Ser?Gln?Leu?Asp?Ala?Lys?Ala?Ile?Ala?Arg?Ala?Ser
145 150 155 160
Leu?Glu?Thr?Ala?Gly?Glu?Ile?Cys?Val?Tyr?Thr?Asn?Asp?Gln?Ile?Ile
165 170 175
Val?Glu?Glu?Leu?Glu
180
<210>65
<211>2263
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1763)
<400>65
aatgaaagag?gatgctgaaa?aacgcgtgaa?atccaacctg?acactggaag?cgatcgccaa 60
agcggaaaac?cttcaagtca?ctgacgaaga?agtcgaagaa?gagctttcta?aaatggctga 120
agcatacaac?atgcctgttg?aaaacatcaa?acaagcaatc?ggttctacag?acggaatgaa 180
agaggactta?aaagttcgca?aagcaattga?ttttcttgta?gaaaaccgtt?aatatgtttc 240
ataatcatac?tttatataaa?acagggcgcg?aatgtttcgt?gccttgtttt?atacaatttt 300
gacttatcat?cttttctttg?ggaaaggata?gataatgtcg?ttttgatcaa?catacatatt 360
gaatatgccg?gacggcaaac?acaacgttat?cgcctttttg?gaaagtgtgg?taaaatgcaa 420
tacataatgg?aacgaatgtt?ttcaagcgat?gtttctttta?acgaagaaac?aggaatgaca 480
aggatataag?gggtgaaaga?atg?ttt?aaa?ttc?aac?gag?gaa?aaa?gga?caa?ttg 533
Met?Phe?Lys?Phe?Asn?Glu?Glu?Lys?Gly?Gln?Leu
1 5 10
aaa?tgc?tcg?ttt?tgc?ggt?aaa?aca?caa?gat?cag?gta?cgc?aag?crt?gtc 581
Lys?Cys?Ser?Phe?Cys?Gly?Lys?Thr?Gln?Asp?Gln?Val?Arg?Lys?Leu?Val
15 20 25
gcc?gga?cca?ggc?gta?tat?ata?tgt?gac?gag?tgc?atc?gaa?ctt?tgc?act 629
Ala?Gly?Pro?Gly?Val?Tyr?Ile?Cys?Asp?Glu?Cys?Ile?Glu?Leu?Cys?Thr
30 35 40
gaa?att?gta?gaa?gag?gaa?ctc?ggc?tct?gaa?gaa?gaa?gtg?gag?ttc?aaa 677
Glu?Ile?Val?Glu?Glu?Glu?Leu?Gly?Ser?Glu?Glu?Glu?Val?Glu?Phe?Lys
45 50 55
gat?gtt?ccg?aag?cca?aag?gaa?atc?cgc?gag?att?ctt?gat?gaa?tac?gtc 725
Asp?Val?Pro?Lys?Pro?Lys?Glu?Ile?Arg?Glu?Ile?Leu?Asp?Glu?Tyr?Val
60 65 70 75
atc?ggc?cag?gac?cag?gca?aaa?aaa?tcc?ctt?gcc?gtg?gcc?gtc?tac?aat 773
Ile?Gly?Gln?Asp?Gln?Ala?Lys?Lys?Ser?Leu?Ala?Val?Ala?Val?Tyr?Asn
80 85 90
cat?tac?aag?cgg?atc?aat?tcg?aac?agc?aaa?gtc?gac?gac?gtc?gag?ctg 821
His?Tyr?Lys?Arg?Ile?Asn?Ser?Asn?Ser?Lys?Val?Asp?Asp?Val?Glu?Leu
95 100 105
tca?aaa?agt?aac?att?tcc?atg?atc?gga?ccg?act?gga?agc?gga?aaa?acg 869
Ser?Lys?Ser?Asn?Ile?Ser?Met?Ile?Gly?Pro?Thr?Gly?Ser?Gly?Lys?Thr
110 115 120
ctt?ctt?gct?caa?acg?ctt?gcc?cgc?atc?ctg?aat?gtg?ccg?ttc?gcc?att 917
Leu?Leu?Ala?Gln?Thr?Leu?Ala?Arg?Ile?Leu?Asn?Val?Pro?Phe?Ala?Ile
125 130 135
gcg?gat?gcc?acg?tcg?ctt?act?gaa?gca?ggc?tat?gtc?ggt?gaa?gat?gtg 965
Ala?Asp?Ala?Thr?Ser?Leu?Thr?Glu?Ala?Gly?Tyr?Val?Gly?Glu?Asp?Val
140 145 150 155
gag?aac?att?ttg?ctt?aag?ctg?atc?cag?gca?gct?gac?tac?gat?gtt?gaa 1013
Glu?Asn?Ile?Leu?Leu?Lys?Leu?Ile?Gln?Ala?Ala?Asp?Tyr?Asp?Val?Glu
160 165 170
aaa?gcg?gaa?aaa?ggc?att?atc?tac?atc?gat?gaa?atc?gat?aaa?gtg?gcc 1061
Lys?Ala?Glu?Lys?Gly?Ile?Ile?Tyr?Ile?Asp?Glu?Ile?Asp?Lys?Val?Ala
175 180 185
aga?aag?tcc?gag?aac?cct?tcg?att?aca?cgc?gat?gta?tca?ggc?gaa?ggc 1109
Arg?Lys?Ser?Glu?Asn?Pro?Ser?Ile?Thr?Arg?Asp?Val?Ser?Gly?Glu?Gly
190 195 200
gtt?cag?cag?gca?ttg?ctt?aaa?att?ctt?gaa?ggt?acg?gtt?gcc?agc?gtt 1157
Val?Gln?Gln?Ala?Leu?Leu?Lys?Ile?Leu?Glu?Gly?Thr?Val?Ala?Ser?Val
205 210 215
cct?cca?caa?ggc?gga?aga?aag?cat?cct?cat?caa?gag?ttc?atc?caa?att 1205
Pro?Pro?Gln?Gly?Gly?Arg?Lys?His?Pro?His?Gln?Glu?Phe?Ile?Gln?Ile
220 225 230 235
gac?aca?acc?aac?atc?ctg?ttc?att?tgc?ggc?gga?gct?ttt?gac?gga?atc 1253
Asp?Thr?Thr?Asn?Ile?Leu?Phe?Ile?Cys?Gly?Gly?Ala?Phe?Asp?Gly?Ile
240 245 250
gag?cag?atc?atc?aag?cgc?cgt?ctc?ggc?cag?aag?gtc?atc?ggc?ttc?ggc 1301
Glu?Gln?Ile?Ile?Lys?Arg?Arg?Leu?Gly?Gln?Lys?Val?Ile?Gly?Phe?Gly
255 260 265
tct?gac?aac?aag?cat?gaa?gac?ctt?gaa?aaa?gaa?gcg?ctt?ctt?tcc?aaa 1349
Ser?Asp?Asn?Lys?His?Glu?Asp?Leu?Glu?Lys?Glu?Ala?Leu?Leu?Ser?Lys
270 275 280
gtg?ctt?ccg?gaa?gac?ttg?ctt?cgc?ttc?ggg?ctg?atc?cct?gaa?ttc?atc 1397
Val?Leu?Pro?Glu?Asp?Leu?Leu?Arg?Phe?Gly?Leu?Ile?Pro?Glu?Phe?Ile
285 290 295
ggc?cgt?ctc?ccg?atc?atc?gca?agc?ctt?gag?ccg?ctg?gat?gaa?aaa?gcg 1445
Gly?Arg?Leu?Pro?Ile?Ile?Ala?Ser?Leu?Glu?Pro?Leu?Asp?Glu?Lys?Ala
300 305 310 315
ctg?gtt?gaa?att?tta?aca?aaa?ccg?aaa?aac?gcg?ctt?gtt?aaa?caa?tat 1493
Leu?Val?Glu?Ile?Leu?Thr?Lys?Pro?Lys?Asn?Ala?Leu?Val?Lys?Gln?Tyr
320 325 330
aga?aaa?atg?ctt?gaa?ttg?gat?gat?gtg?gag?ctt?gtg?ttt?gaa?gac?gaa 1541
Arg?Lys?Met?Leu?Glu?Leu?Asp?Asp?Val?Glu?Leu?Val?Phe?Glu?Asp?Glu
335 340 345
gcg?ctc?aca?gaa?atc?gct?aaa?aaa?gca?att?gaa?cgt?aaa?aca?gga?gca 1589
Ala?Leu?Thr?Glu?Ile?Ala?Lys?Lys?Ala?Ile?Glu?Arg?Lys?Thr?Gly?Ala
350 355 360
cgc?gga?ctt?cgt?tcc?att?atc?gaa?gga?atc?atg?ctc?gac?gtg?atg?ttt 1637
Arg?Gly?Leu?Arg?Ser?Ile?Ile?Glu?Gly?Ile?Met?Leu?Asp?Val?Met?Phe
365 370 375
gat?ctg?ccg?tct?cgc?gag?gat?att?gaa?aaa?tgc?gtg?atc?aca?ggc?aaa 1685
Asp?Leu?Pro?Ser?Arg?Glu?Asp?Ile?Glu?Lys?Cys?Val?Ile?Thr?Gly?Lys
380 385 390 395
acc?gtc?act?gac?ggc?gaa?ccg?ccg?cgt?ctc?att?atg?aaa?gac?ggc?acg 1733
Thr?Val?Thr?Asp?Gly?Glu?Pro?Pro?Arg?Leu?Ile?Met?Lys?Asp?Gly?Thr
400 405 410
gtc?gtc?aat?aaa?gac?aaa?aaa?act?tca?gca?taatgcagag?aacctcctgg 1783
Val?Val?Asn?Lys?Asp?Lys?Lys?Thr?Ser?Ala
415 420
cctaaaggag?gttctttttt?tgcatccgga?aaccgtcggc?gtcccggagc?agagtgacgg 1843
cttcaaaagc?aaaataatcc?ctttctgtac?gtttattccc?tccctgatca?ggaaatacta 1903
tctgacatac?taaagatacg?aaaccatttc?aggagggacc?atcagttgag?ctggacgagc 1963
attgcacttt?ttgtacaact?atttttcggg?atcatcatcg?gattatattt?ttggaatttg 2023
ctcaaaaatc?aaagaaccca?aaaggttacg?atagacagag?agtccaaaaa?agagatggaa 2083
cagctccgga?aaatgagatc?aatccattta?tcagaacctt?tatcggaaaa?ggtgaggccg 2143
acggctttta?aagacatcgt?cggccaggag?gacggcatta?aagcgctgaa?agccgccctt 2203
tgcggaccga?atccgcagca?tgtcattatt?tacggccctc?caggtgtggg?aaaaacggca 2263
<210>66
<211>421
<212>PRT
＜213〉Bacillus licheniformis
<400>66
Met?Phe?Lys?Phe?Asn?Glu?Glu?Lys?Gly?Gln?Leu?Lys?Cys?Ser?Phe?Cys
1 5 10 15
Gly?Lys?Thr?Gln?Asp?Gln?Val?Arg?Lys?Leu?Val?Ala?Gly?Pro?Gly?Val
20 25 30
Tyr?Ile?Cys?Asp?Glu?Cys?Ile?Glu?Leu?Cys?Thr?Glu?Ile?Val?Glu?Glu
35 40 45
Glu?Leu?Gly?Ser?Glu?Glu?Glu?Val?Glu?Phe?Lys?Asp?Val?Pro?Lys?Pro
50 55 60
Lys?Glu?Ile?Arg?Glu?Ile?Leu?Asp?Glu?Tyr?Val?Ile?Gly?Gln?Asp?Gln
65 70 75 80
Ala?Lys?Lys?Ser?Leu?Ala?Val?Ala?Val?Tyr?Asn?His?Tyr?Lys?Arg?Ile
85 90 95
Asn?Ser?Asn?Ser?Lys?Val?Asp?Asp?Val?Glu?Leu?Ser?Lys?Ser?Asn?Ile
100 105 110
Ser?Met?Ile?Gly?Pro?Thr?Gly?Ser?Gly?Lys?Thr?Leu?Leu?Ala?Gln?Thr
115 120 125
Leu?Ala?Arg?Ile?Leu?Asn?Val?Pro?Phe?Ala?Ile?Ala?Asp?Ala?Thr?Ser
130 135 140
Leu?Thr?Glu?Ala?Gly?Tyr?Val?Gly?Glu?Asp?Val?Glu?Asn?Ile?Leu?Leu
145 150 155 160
Lys?Leu?Ile?Gln?Ala?Ala?Asp?Tyr?Asp?Val?Glu?Lys?Ala?Glu?Lys?Gly
165 170 175
Ile?Ile?Tyr?Ile?Asp?Glu?Ile?Asp?Lys?Val?Ala?Arg?Lys?Ser?Glu?Asn
180 185 190
Pro?Ser?Ile?Thr?Arg?Asp?Val?Ser?Gly?Glu?Gly?Val?Gln?Gln?Ala?Leu
195 200 205
Leu?Lys?Ile?Leu?Glu?Gly?Thr?Val?Ala?Ser?Val?Pro?Pro?Gln?Gly?Gly
210 215 220
Arg?Lys?His?Pro?His?Gln?Glu?Phe?Ile?Gln?Ile?Asp?Thr?Thr?Asn?Ile
225 230 235 240
Leu?Phe?Ile?Cys?Gly?Gly?Ala?Phe?Asp?Gly?Ile?Glu?Gln?Ile?Ile?Lys
245 250 255
Arg?Arg?Leu?Gly?Gln?Lys?Val?Ile?Gly?Phe?Gly?Ser?Asp?Asn?Lys?His
260 265 270
Glu?Asp?Leu?Glu?Lys?Glu?Ala?Leu?Leu?Ser?Lys?Val?Leu?Pro?Glu?Asp
275 280 285
Leu?Leu?Arg?Phe?Gly?Leu?Ile?Pro?Glu?Phe?Ile?Gly?Arg?Leu?Pro?Ile
290 295 300
Ile?Ala?Ser?Leu?Glu?Pro?Leu?Asp?Glu?Lys?Ala?Leu?Val?Glu?Ile?Leu
305 310 315 320
Thr?Lys?Pro?Lys?Asn?Ala?Leu?Val?Lys?Gln?Tyr?Arg?Lys?Met?Leu?Glu
325 330 335
Leu?Asp?Asp?Val?Glu?Leu?Val?Phe?Glu?Asp?Glu?Ala?Leu?Thr?Glu?Ile
340 345 350
Ala?Lys?Lys?Ala?Ile?Glu?Arg?Lys?Thr?Gly?Ala?Arg?Gly?Leu?Arg?Ser
355 360 365
Ile?Ile?Glu?Gly?Ile?Met?Leu?Asp?Val?Met?Phe?Asp?Leu?Pro?Ser?Arg
370 375 380
Glu?Asp?Ile?Glu?Lys?Cys?Val?Ile?Thr?Gly?Lys?Thr?Val?Thr?Asp?Gly
385 390 395 400
Glu?Pro?Pro?Arg?Leu?Ile?Met?Lys?Asp?Gly?Thr?Val?Val?Asn?Lys?Asp
405 410 415
Lys?Lys?Thr?Ser?Ala
420
<210>67
<211>2653
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(2153)
<400>67
gttgaaattt?taacaaaacc?gaaaaacgcg?cttgttaaac?aatatagaaa?aatgcttgaa 60
ttggatgatg?tggagcttgt?gtttgaagac?gaagcgctca?cagaaatcgc?taaaaaagca 120
attgaacgta?aaacaggagc?acgcggactt?cgttccatta?tcgaaggaat?catgctcgac 180
gtgatgtttg?atctgccgtc?tcgcgaggat?attgaaaaat?gcgtgatcac?aggcaaaacc 240
gtcactgacg?gcgaaccgcc?gcgtctcatt?atgaaagacg?gcacggtcgt?caataaagac 300
aaaaaaactt?cagcataatg?cagagaacct?cctggcctaa?aggaggttct?ttttttgcat 360
ccggaaaccg?tcggcatccc?ggagcagagt?gacggcttca?aaagcaaaat?aatccctttc 420
tgtacgttta?ttccctccct?gatcaggaaa?tactatctga?catactaaag?atacgaaacc 480
atttcaggag?ggaccatcag?ttg?agc?tgg?acg?agc?att?gca?ctt?ttt?gta?caa 533
Leu?Ser?Trp?Thr?Ser?Ile?Ala?Leu?Phe?Val?Gln
1 5 10
cta?ttt?ttc?ggg?atc?atc?atc?gga?tta?tat?ttt?tgg?aat?ttg?ctc?aaa 581
Leu?Phe?Phe?Gly?Ile?Ile?Ile?Gly?Leu?Tyr?Phe?Trp?Asn?Leu?Leu?Lys
15 20 25
aat?caa?aga?acc?caa?aag?gtt?acg?ata?gac?aga?gag?tcc?aaa?aaa?gag 629
Asn?Gln?Arg?Thr?Gln?Lys?Val?Thr?Ile?Asp?Arg?Glu?Ser?Lys?Lys?Glu
30 35 40
atg?gaa?cag?ctc?cgg?aaa?atg?aga?tca?atc?cat?tta?tca?gaa?cct?tta 677
Met?Glu?Gln?Leu?Arg?Lys?Met?Arg?Ser?Ile?His?Leu?Ser?Glu?Pro?Leu
45 50 55
tcg?gaa?aag?gtg?agg?ccg?acg?gct?ttt?aaa?gac?atc?gtc?ggc?cag?gag 725
Ser?Glu?Lys?Val?Arg?Pro?Thr?Ala?Phe?Lys?Asp?Ile?Val?Gly?Gln?Glu
60 65 70 75
gac?ggc?att?aaa?gcg?ctg?aaa?gcc?gcc?ctt?tgc?gga?ccg?aat?ccg?cag 773
Asp?Gly?Ile?Lys?Ala?Leu?Lys?Ala?Ala?Leu?Cys?Gly?Pro?Asn?Pro?Gln
80 85 90
cat?gtc?att?att?tac?ggc?cct?cca?ggt?gtg?gga?aaa?acg?gca?gct?gca 821
His?Val?Ile?Ile?Tyr?Gly?Pro?Pro?Gly?Val?Gly?Lys?Thr?Ala?Ala?Ala
95 100 105
agg?ctt?gtc?ctt?gaa?gaa?gcg?aaa?aag?aac?aag?cgc?tea?ccg?ttc?cag 869
Arg?Leu?Val?Leu?Glu?Glu?Ala?Lys?Lys?Asn?Lys?Arg?Ser?Pro?Phe?Gln
110 115 120
gaa?cat?tcc?gcc?ttt?gtc?gag?ctt?gac?gcg?aca?acg?gcc?cgt?ttc?gac 917
Glu?His?Ser?Ala?Phe?Val?Glu?Leu?Asp?Ala?Thr?Thr?Ala?Arg?Phe?Asp
125 130 135
gag?cgg?gga?atc?gcc?gac?ccg?ttg?atc?ggt?tcg?gtc?cac?gat?ccg?att 965
Glu?Arg?Gly?Ile?Ala?Asp?Pro?Leu?Ile?Gly?Ser?Val?His?Asp?Pro?Ile
140 145 150 155
tat?cag?ggc?gcc?gga?gcg?atg?ggg?cag?gcc?gga?att?ccc?cag?cca?aag 1013
Tyr?Gln?Gly?Ala?Gly?Ala?Met?Gly?Gln?Ala?Gly?Ile?Pro?Gln?Pro?Lys
160 165 170
cag?gga?gcc?gtc?acg?cat?gca?cac?ggt?gga?gta?tta?ttc?atc?gat?gaa 1061
Gln?Gly?Ala?Val?Thr?His?Ala?His?Gly?Gly?Val?Leu?Phe?Ile?Asp?Glu
175 180 185
atc?gga?gag?ctt?cat?ccg?att?caa?atg?aat?aaa?atg?ctg?aag?gtg?ctc 1109
Ile?Gly?Glu?Leu?His?Pro?Ile?Gln?Met?Asn?Lys?Met?Leu?Lys?Val?Leu
190 195 200
gaa?gat?cgg?aaa?gtt?ttt?ttg?gaa?agc?gcc?tat?tac?agt?gaa?gaa?aac 1157
Glu?Asp?Arg?Lys?Val?Phe?Leu?Glu?Ser?Ala?Tyr?Tyr?Ser?Glu?Glu?Asn
205 210 215
acg?caa?atc?ccc?aag?cat?att?cac?gac?att?ttt?caa?aac?ggg?ctg?ccg 1205
Thr?Gln?Ile?Pro?Lys?His?Ile?His?Asp?Ile?Phe?Gln?Asn?Gly?Leu?Pro
220 225 230 235
gcg?gat?ttc?aga?ctg?atc?ggc?gcg?acg?aca?agg?atg?cct?gat?gaa?atc 1253
Ala?Asp?Phe?Arg?Leu?Ile?Gly?Ala?Thr?Thr?Arg?Met?Pro?Asp?Glu?Ile
240 245 250
ccg?cct?gcc?att?cgg?tcg?aga?tgt?ttg?gaa?gtg?ttc?ttc?cgc?gac?ctt 1301
Pro?Pro?Ala?Ile?Arg?Ser?Arg?Cys?Leu?Glu?Val?Phe?Phe?Arg?Asp?Leu
255 260 265
gaa?aag?cac?gaa?tta?aaa?acg?gtc?gcc?aaa?aaa?gcg?gca?gat?aag?att 1349
Glu?Lys?His?Glu?Leu?Lys?Thr?Val?Ala?Lys?Lys?Ala?Ala?Asp?Lys?Ile
270 275 280
caa?aag?aaa?gtc?gca?gag?gaa?gga?ctc?gac?ctg?ttg?acg?cgg?tat?gcg 1397
Gln?Lys?Lys?Val?Ala?Glu?Glu?Gly?Leu?Asp?Leu?Leu?Thr?Arg?Tyr?Ala
285 290 295
aga?aac?ggc?cgc?gaa?gtc?gtc?aat?atg?atg?caa?atc?gcc?gcc?gga?atg 1445
Arg?Asn?Gly?Arg?Glu?Val?Val?Asn?Met?Met?Gln?Ile?Ala?Ala?Gly?Met
300 305 310 315
gct?ttg?act?gaa?gag?cgc?gac?gag?gtg?acg?gtt?gaa?gat?atc?gaa?tgg 1493
Ala?Leu?Thr?Glu?Glu?Arg?Asp?Glu?Val?Thr?Val?Glu?Asp?Ile?Glu?Trp
320 325 330
gtg?atc?cat?tcg?agc?cag?ctg?aca?ccg?aaa?tac?gaa?caa?aaa?atc?gcc 1541
Val?Ile?His?Ser?Ser?Gln?Leu?Thr?Pro?Lys?Tyr?Glu?Gln?Lys?Ile?Ala
335 340 345
tct?aaa?ccg?cag?gtc?ggc?atc?gtc?aac?gga?ctt?gcc?gtc?cac?ggg?cct 1589
Ser?Lys?Pro?Gln?Val?Gly?Ile?Val?Asn?Gly?Leu?Ala?Val?His?Gly?Pro
350 355 360
aac?agc?ggt?tcg?ctg?ctc?gaa?att?gag?gtc?aca?gtc?aat?cag?gcg?gct 1637
Asn?Ser?Gly?Ser?Leu?Leu?Glu?Ile?Glu?Val?Thr?Val?Asn?Gln?Ala?Ala
365 370 375
gat?aaa?gga?tcg?att?aat?atc?aca?ggc?att?gca?gaa?gaa?gag?aat?atc 1685
Asp?Lys?Gly?Ser?Ile?Asn?Ile?Thr?Gly?Ile?Ala?Glu?Glu?Glu?Asn?Ile
380 385 390 395
ggc?aac?cag?tca?aaa?tcg?atc?cgc?cgg?aaa?agc?atg?gcg?aaa?gga?tcg 1733
Gly?Asn?Gln?Ser?Lys?Ser?Ile?Arg?Arg?Lys?Ser?Met?Ala?Lys?Gly?Ser
400 405 410
gtc?gaa?aac?gtc?atg?act?gta?ttg?cgg?aca?atg?gga?gtc?aaa?gct?tcg 1781
Val?Glu?Asn?Val?Met?Thr?Val?Leu?Arg?Thr?Met?Gly?Val?Lys?Ala?Ser
415 420 425
gat?tat?gat?att?cac?gtg?aat?ttc?ccc?ggc?ggt?atc?ccg?gtt?gac?gga 1829
Asp?Tyr?Asp?Ile?His?Val?Asn?Phe?Pro?Gly?Gly?Ile?Pro?Val?Asp?Gly
430 435 440
cca?tct?gca?ggg?att?gca?atg?gcg?gcg?ggc?ata?ttc?tcc?gct?att?cat 1877
Pro?Ser?Ala?Gly?Ile?Ala?Met?Ala?Ala?Gly?Ile?Phe?Ser?Ala?Ile?His
445 450 455
aag?att?ccg?ata?gac?cat?act?gtc?gcc?atg?acg?ggt?gaa?atc?agc?ctg 1925
Lys?Ile?Pro?Ile?Asp?His?Thr?Val?Ala?Met?Thr?Gly?Glu?Ile?Ser?Leu
460 465 470 475
aac?ggc?cgg?gta?aag?ccg?atc?ggc?ggc?gtg?att?ccg?aaa?atc?aaa?gcg 1973
Asn?Gly?Arg?Val?Lys?Pro?Ile?Gly?Gly?Val?Ile?Pro?Lys?Ile?Lys?Ala
480 485 490
gcc?aag?cat?gcg?ggg?gca?aaa?aca?gtc?att?att?ccg?cac?gaa?aat?gtt 2021
Ala?Lys?His?Ala?Gly?Ala?Lys?Thr?Val?Ile?Ile?Pro?His?Glu?Asn?Val
495 500 505
cag?tcg?att?tta?aag?cgg?ata?gaa?ggc?ata?cat?atc?gtt?ccc?gtc?aaa 2069
Gln?Ser?Ile?Leu?Lys?Arg?Ile?Glu?Gly?Ile?His?Ile?Val?Pro?Val?Lys
510 515 520
acc?ttg?cag?gaa?gta?ctc?gac?ctc?gta?ttg?gtc?aac?cct?cct?tca?gaa 2117
Thr?Leu?Gln?Glu?Val?Leu?Asp?Leu?Val?Leu?Val?Asn?Pro?Pro?Ser?Glu
525 530 535
atg?ccc?gac?ggc?gtt?caa?atg?aaa?aga?gaa?tcc?gtt?taaatcccca 2163
Met?Pro?Asp?Gly?Val?Gln?Met?Lys?Arg?Glu?Ser?Val
540 545 550
tcgtttcaga?tggggatttt?tttcggggcg?gtttctccga?agccgggagc?cggcatgttt 2223
tcttagcgga?ggacatcttt?tcctttttca?gaactgaaag?aaagggtata?ctacaggaga 2283
ccctttttat?ttcgccgggc?ttacaattgt?acaggaataa?aaaaagtata?ttataatggt 2343
tcatactaaa?gttacggagg?tgtcagtcaa?aatggcagat?gaaacgaaac?gaaacatccc 2403
gctcctccct?ttaagaggtt?tactcgtcta?tccgacgatg?gttttgcacc?ttgacgtcgg 2463
gcgtgaaaag?tcggttcagg?cacttgaaca?ggcgatgatg?aatgaccata?tgatcttttt 2523
ggcaacgcaa?aaggacatat?ccatagacga?accggatgaa?gacgagattt?ttacattcgg 2583
aacctatacg?aaaatcaagc?agatgctcaa?gctgccgaac?ggaacaatcc?gcgttttggt 2643
ggaggggttg 2653
<210>68
<211>551
<212>PRT
＜213〉Bacillus licheniformis
<400>68
Leu?Ser?Trp?Thr?Ser?Ile?Ala?Leu?Phe?Val?Gln?Leu?Phe?Phe?Gly?Ile
1 5 10 15
Ile?Ile?Gly?Leu?Tyr?Phe?Trp?Asn?Leu?Leu?Lys?Asn?Gln?Arg?Thr?Gln
20 25 30
Lys?Val?Thr?Ile?Asp?Arg?Glu?Ser?Lys?Lys?Glu?Met?Glu?Gln?Leu?Arg
35 40 45
Lys?Met?Arg?Ser?Ile?His?Leu?Ser?Glu?Pro?Leu?Ser?Glu?Lys?Val?Arg
50 55 60
Pro?Thr?Ala?Phe?Lys?Asp?Ile?Val?Gly?Gln?Glu?Asp?Gly?Ile?Lys?Ala
65 70 75 80
Leu?Lys?Ala?Ala?Leu?Cys?Gly?Pro?Asn?Pro?Gln?His?Val?Ile?Ile?Tyr
85 90 95
Gly?Pro?Pro?Gly?Val?Gly?Lys?Thr?Ala?Ala?Ala?Arg?Leu?Val?Leu?Glu
100 105 110
Glu?Ala?Lys?Lys?Asn?Lys?Arg?Ser?Pro?Phe?Gln?Glu?His?Ser?Ala?Phe
115 120 125
Val?Glu?Leu?Asp?Ala?Thr?Thr?Ala?Arg?Phe?Asp?Glu?Arg?Gly?Ile?Ala
130 135 140
Asp?Pro?Leu?Ile?Gly?Ser?Val?His?Asp?Pro?Ile?Tyr?Gln?Gly?Ala?Gly
145 150 155 160
Ala?Met?Gly?Gln?Ala?Gly?Ile?Pro?Gln?Pro?Lys?Gln?Gly?Ala?Val?Thr
165 170 175
His?Ala?His?Gly?Gly?Val?Leu?Phe?Ile?Asp?Glu?Ile?Gly?Glu?Leu?His
180 185 190
Pro?Ile?Gln?Met?Asn?Lys?Met?Leu?Lys?Val?Leu?Glu?Asp?Arg?Lys?Val
l95 200 205
Phe?Leu?Glu?Ser?Ala?Tyr?Tyr?Ser?Glu?Glu?Asn?Thr?Gln?Ile?Pro?Lys
210 215 220
His?Ile?His?Asp?Ile?Phe?Gln?Asn?Gly?Leu?Pro?Ala?Asp?Phe?Arg?Leu
225 230 235 240
Ile?Gly?Ala?Thr?Thr?Arg?Met?Pro?Asp?Glu?Ile?Pro?Pro?Ala?Ile?Arg
245 250 255
Ser?Arg?Cys?Leu?Glu?Val?Phe?Phe?Arg?Asp?Leu?Glu?Lys?His?Glu?Leu
260 265 270
Lys?Thr?Val?Ala?Lys?Lys?Ala?Ala?Asp?Lys?Ile?Gln?Lys?Lys?Val?Ala
275 280 285
Glu?Glu?Gly?Leu?Asp?Leu?Leu?Thr?Arg?Tyr?Ala?Arg?Asn?Gly?Arg?Glu
290 295 300
Val?Val?Asn?Met?Met?Gln?Ile?Ala?Ala?Gly?Met?Ala?Leu?Thr?Glu?Glu
305 310 315 320
Arg?Asp?Glu?Val?Thr?Val?Glu?Asp?Ile?Glu?Trp?Val?Ile?His?Ser?Ser
325 330 335
Gln?Leu?Thr?Pro?Lys?Tyr?Glu?Gln?Lys?Ile?Ala?Ser?Lys?Pro?Gln?Val
340 345 350
Gly?Ile?Val?Asn?Gly?Leu?Ala?Val?His?Gly?Pro?Asn?Ser?Gly?Ser?Leu
355 360 365
Leu?Glu?Ile?Glu?Val?Thr?Val?Asn?Gln?Ala?Ala?Asp?Lys?Gly?Ser?Ile
370 375 380
Asn?Ile?Thr?Gly?Ile?Ala?Glu?Glu?Glu?Asn?Ile?Gly?Asn?Gln?Ser?Lys
385 390 395 400
Ser?Ile?Arg?Arg?Lys?Ser?Met?Ala?Lys?Gly?Ser?Val?Glu?Asn?Val?Met
405 410 415
Thr?Val?Leu?Arg?Thr?Met?Gly?Val?Lys?Ala?Ser?Asp?Tyr?Asp?Ile?His
420 425 430
Val?Asn?Phe?Pro?Gly?Gly?Ile?Pro?Val?Asp?Gly?Pro?Ser?Ala?Gly?Ile
435 440 445
Ala?Met?Ala?Ala?Gly?Ile?Phe?Ser?Ala?Ile?His?Lys?Ile?Pro?Ile?Asp
450 455 460
His?Thr?Val?Ala?Met?Thr?Gly?Glu?Ile?Ser?Leu?Asn?Gly?Arg?Val?Lys
465 470 475 480
Pro?Ile?Gly?Gly?Val?Ile?Pro?Lys?Ile?Lys?Ala?Ala?Lys?His?Ala?Gly
485 490 495
Ala?Lys?Thr?Val?Ile?Ile?Pro?His?Glu?Asn?Val?Gln?Ser?Ile?Leu?Lys
500 505 510
Arg?Ile?Glu?Gly?Ile?His?Ile?Val?Pro?Val?Lys?Thr?Leu?Gln?Glu?Val
515 520 525
Leu?Asp?Leu?Val?Leu?Val?Asn?Pro?Pro?Ser?Glu?Met?Pro?Asp?Gly?Val
530 535 540
Gln?Met?Lys?Arg?Glu?Ser?Val
545 550
<210>69
<211>2230
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1730)
<400>69
cagagacaag?atctttcatt?ttacgaatca?aacggtattc?aggggggcga?ttatttgcac 60
ccaaataaac?ataaaaatcc?ttcaatataa?agactgctgg?ctgatcatat?ttttcaatga 120
acgccaaagc?ctttaacggg?tcttttgttt?cggctgatgg?aggcattcca?ttgctttcaa 180
accctttggt?taaactccaa?acaaataagg?ttcttggtgt?tctaatcaat?tctgtatccg 240
aaacaatatg?actcagttga?tctatgacac?gttcttcttc?ccatgtagaa?atatacagga 300
acggaaagcg?ggccctgaaa?taattcgcta?cttcttttct?aaagtgatga?tatccttcca 360
tttttctccc?tttctattga?cctatttttt?attattatac?taaatttttc?cacaacagga 420
ataaaagattt?caatcctcc?cattcgcctt?ctctaaatga?taagattaaa?ataaaaccaa 480
caattggaga?tgaaggaaac?atg?aag?aac?aaa?ttg?att?gaa?cgc?ctc?att?tct 533
Met?Lys?Asn?Lys?Leu?Ile?Glu?Arg?Leu?Ile?Ser
1 5 10
tat?gcg?aaa?gtg?gat?acg?cag?tca?aat?gaa?aac?agt?cag?acg?acg?cct 581
Tyr?Ala?Lys?Val?Asp?Thr?Gln?Ser?Asn?Glu?Asn?Ser?Gln?Thr?Thr?Pro
15 20 25
tcc?act?ccc?ggg?cag?ctg?gcg?ctt?gcc?aat?atg?ctt?gtc?gaa?gag?ctg 629
Ser?Thr?Pro?Gly?Gln?Leu?Ala?Leu?Ala?Asn?Met?Leu?Val?Glu?Glu?Leu
30 35 40
aaa?gag?atc?ggc?atg?aag?gac?gtc?aca?atc?gat?gaa?aac?ggc?tat?gtg 677
Lys?Glu?Ile?Gly?Met?Lys?Asp?Val?Thr?Ile?Asp?Glu?Asn?Gly?Tyr?Val
45 50 55
atg?gcg?acg?ctt?cct?tcg?aat?aca?gaa?aaa?gag?gtg?ccg?acg?atc?ggt 725
Met?Ala?Thr?Leu?Pro?Ser?Asn?Thr?Glu?Lys?Glu?Val?Pro?Thr?Ile?Gly
60 65 70 75
ttc?ttg?gct?cat?gtg?gat?aca?gca?aca?gat?ttt?acc?gga?aag?aac?gtc 773
Phe?Leu?Ala?His?Val?Asp?Thr?Ala?Thr?Asp?Phe?Thr?Gly?Lys?Asn?Val
80 85 90
aat?ccg?cag?gtt?atc?gaa?caa?tac?gac?gga?aag?gat?att?gtg?ctg?aat 821
Asn?Pro?Gln?Val?Ile?Glu?Gln?Tyr?Asp?Gly?Lys?Asp?Ile?Val?Leu?Asn
95 100 105
gaa?tcc?ctc?aat?gtc?gtc?tta?tcg?ccc?aag?gaa?ttc?ccc?gag?cta?gca 869
Glu?Ser?Leu?Asn?Val?Val?Leu?Ser?Pro?Lys?Glu?Phe?Pro?Glu?Leu?Ala
110 115 120
gat?tat?gcg?gga?cat?acg?ttg?att?aca?acc?gac?gga?acg?act?ttg?ctc 917
Asp?Tyr?Ala?Gly?His?Thr?Leu?Ile?Thr?Thr?Asp?Gly?Thr?Thr?Leu?Leu
125 130 135
ggc?gcc?gac?aat?aaa?gct?ggg?atc?tct?gaa?atc?atg?acg?gca?atg?gaa 965
Gly?Ala?Asp?Asn?Lys?Ala?Gly?Ile?Ser?Glu?Ile?Met?Thr?Ala?Met?Glu
140 145 150 155
tat?tta?att?gcg?cac?ccc?gaa?atc?aaa?cac?ggg?aag?atc?aga?gtc?gct 1013
Tyr?Leu?Ile?Ala?His?Pro?Glu?Ile?Lys?His?Gly?Lys?Ile?Arg?Val?Ala
160 165 170
ttt?aca?cct?gat?gaa?gag?atc?ggc?aga?ggg?ccg?cac?aag?ttt?gac?gtc 1061
Phe?Thr?Pro?Asp?Glu?Glu?Ile?Gly?Arg?Gly?Pro?His?Lys?Phe?Asp?Val
175 180 185
gag?gcg?ttc?aat?gcc?aaa?ttc?gcg?tat?acg?gtt?gac?ggc?gga?ccg?ctt 1109
Glu?Ala?Phe?Asn?Ala?Lys?Phe?Ala?Tyr?Thr?Val?Asp?Gly?Gly?Pro?Leu
190 195 200
ggc?gaa?ctg?cag?tac?gaa?agc?ttc?aat?gcc?gca?gcc?gcg?aaa?atc?act 1157
Gly?Glu?Leu?Gln?Tyr?Glu?Ser?Phe?Asn?Ala?Ala?Ala?Ala?Lys?Ile?Thr
205 210 215
tgt?aaa?gga?acg?aac?gtc?cac?ccc?ggc?aca?gca?aaa?gga?aaa?atg?gtc 1205
Cys?Lys?Gly?Thr?Asn?Val?His?Pro?Gly?Thr?Ala?Lys?Gly?Lys?Met?Val
220 225 230 235
aat?gcc?gcc?aaa?atc?gcg?atg?cag?ttc?cac?gcc?gca?ttg?ccg?gaa?aac 1253
Asn?Ala?Ala?Lys?Ile?Ala?Met?Gln?Phe?His?Ala?Ala?Leu?Pro?Glu?Asn
240 245 2 50
gaa?gct?cct?gaa?ttt?aca?gaa?ggt?tat?gaa?ggc?ttt?tat?cat?ctg?ctt 1301
Glu?Ala?Pro?Glu?Phe?Thr?Glu?Gly?Tyr?Glu?Gly?Phe?Tyr?His?Leu?Leu
255 260 265
tca?atc?aag?ggc?gat?gtt?tct?gaa?acg?agc?ctc?tct?tat?atc?att?aga 1349
Ser?Ile?Lys?Gly?Asp?Val?Ser?Glu?Thr?Ser?Leu?Ser?Tyr?Ile?Ile?Arg
270 275 280
gat?ttt?gac?aga?gac?cga?ttt?aac?gag?aga?aaa?gac?acc?gtc?caa?aaa 1397
Asp?Phe?Asp?Arg?Asp?Arg?Phe?Asn?Glu?Arg?Lys?Asp?Thr?Val?Gln?Lys
285 290 295
atc?gca?aac?aac?ctt?aaa?gcg?aaa?tac?ggc?gaa?aac?agc?gtc?aca?gtg 1445
Ile?Ala?Asn?Asn?Leu?Lys?Ala?Lys?Tyr?Gly?Glu?Asn?Ser?Val?Thr?Val
300 305 310 315
gac?atg?aat?gat?caa?tat?tac?aac?atg?agg?gaa?aag?atc?gaa?ccg?gtc 1493
Asp?Met?Asn?Asp?Gln?Tyr?Tyr?Asn?Met?Arg?Glu?Lys?Ile?Glu?Pro?Val
320 325 330
aaa?gag?att?gtc?gac?att?gcc?tat?aaa?gcg?atg?aaa?aac?ctt?gat?att 1541
Lys?Glu?Ile?Val?Asp?Ile?Ala?Tyr?Lys?Ala?Met?Lys?Asn?Leu?Asp?Ile
335 340 345
gaa?ccg?gtc?gtt?aaa?ccg?atc?cgc?ggc?ggt?aca?gac?ggc?tct?cag?ctc 1589
Glu?Pro?Val?Val?Lys?Pro?Ile?Arg?Gly?Gly?Thr?Asp?Gly?Ser?Gln?Leu
350 355 360
tca?tat?atg?ggg?ctt?cct?tgt?ccg?aac?att?ttc?aca?ggc?ggg?gaa?aac 1637
Ser?Tyr?Met?Gly?Leu?Pro?Cys?Pro?Asn?Ile?Phe?Thr?Gly?Gly?Glu?Asn
365 370 375
ttt?cac?gga?aaa?tac?gag?tat?att?tcc?gcc?gac?aat?atg?gta?aaa?gcc 1685
Phe?His?Gly?Lys?Tyr?Glu?Tyr?Ile?Ser?Ala?Asp?Asn?Met?Val?Lys?Ala
380 385 390 395
gcg?aat?gtc?atc?gtg?gaa?att?gtg?aag?ctg?ttt?gaa?gaa?aga?gct 1730
Ala?Asn?Val?Ile?Val?Glu?Ile?Val?Lys?Leu?Phe?Glu?Glu?Arg?Ala
400 405 410
taaacgaaaa?aagcggaaag?cggctgcaag?acaaagcagc?cgcttttttt?tccagatcaa 1790
tctttatctg?cgctttgctg?aaattagaac?atgttgcaag?gcggtttctt?tttggagcgg 1850
gataaagcca?attaacatct?caccgatgca?aaaaattctg?ttcatttggc?ttcatcacct 1910
ttccaaatat?ctttcaacgt?ccagtaagtg?aattcttcaa?cccgtacgtt?gcctttctca 1970
gaaaacagct?caatccccgc?tcttccttcc?ttcggatata?tgcggcttgt?cattgtcgct 2030
tcaccatgat?ttgcaaatac?ttcaatagac?gatctgtcga?gaaacagatg?caaagtgagc 2090
ttctcatccg?cttcaagccg?tacgtttctc?acaccgtccc?tcgctttgcc?ggactttgaa 2150
cagtcgagcg?tcagcttttg?atcaatcaag?ctgtacttga?tggttgtttc?ttcctgttca 2210
attccccgga?tctttaagcc 2230
<210>70
<211>410
<212>PRT
＜213〉Bacillus licheniformis
<400>70
Met?Lys?Asn?Lys?Leu?Ile?Glu?Arg?Leu?Ile?Ser?Tyr?Ala?Lys?Val?Asp
1 5 10 15
Thr?Gln?Ser?Asn?Glu?Asn?Ser?Gln?Thr?Thr?Pro?Ser?Thr?Pro?Gly?Gln
20 25 30
Leu?Ala?Leu?Ala?Asn?Met?Leu?Val?Glu?Glu?Leu?Lys?Glu?Ile?Gly?Met
35 40 45
Lys?Asp?Val?Thr?Ile?Asp?Glu?Asn?Gly?Tyr?Val?Met?Ala?Thr?Leu?Pro
50 55 60
Ser?Asn?Thr?Glu?Lys?Glu?Val?Pro?Thr?Ile?Gly?Phe?Leu?Ala?His?Val
65 70 75 80
Asp?Thr?Ala?Thr?Asp?Phe?Thr?Gly?Lys?Asn?Val?Asn?Pro?Gln?Val?Ile
85 90 95
Glu?Gln?Tyr?Asp?Gly?Lys?Asp?Ile?Val?Leu?Asn?Glu?Ser?Leu?Asn?Val
100 105 110
Val?Leu?Ser?Pro?Lys?Glu?Phe?Pro?Glu?Leu?Ala?Asp?Tyr?Ala?Gly?His
115 120 125
Thr?Leu?Ile?Thr?Thr?Asp?Gly?Thr?Thr?Leu?Leu?Gly?Ala?Asp?Asn?Lys
130 135 140
Ala?Gly?Ile?Ser?Glu?Ile?Met?Thr?Ala?Met?Glu?Tyr?Leu?Ile?Ala?His
145 150 155 160
Pro?Glu?Ile?Lys?His?Gly?Lys?Ile?Arg?Val?Ala?Phe?Thr?Pro?Asp?Glu
165 170 175
Glu?Ile?Gly?Arg?Gly?Pro?His?Lys?Phe?Asp?Val?Glu?Ala?Phe?Asn?Ala
180 185 190
Lys?Phe?Ala?Tyr?Thr?Val?Asp?Gly?Gly?Pro?Leu?Gly?Glu?Leu?Gln?Tyr
195 200 205
Glu?Ser?Phe?Asn?Ala?Ala?Ala?Ala?Lys?Ile?Thr?Cys?Lys?Gly?Thr?Asn
210 215 220
Val?His?Pro?Gly?Thr?Ala?Lys?Gly?Lys?Met?Val?Asn?Ala?Ala?Lys?Ile
225 230 235 240
Ala?Met?Gln?Phe?His?Ala?Ala?Leu?Pro?Glu?Asn?Glu?Ala?Pro?Glu?Phe
245 250 255
Thr?Glu?Gly?Tyr?Glu?Gly?Phe?Tyr?His?Leu?Leu?Ser?Ile?Lys?Gly?Asp
260 265 270
Val?Ser?Glu?Thr?Ser?Leu?Ser?Tyr?Ile?Ile?Arg?Asp?Phe?Asp?Arg?Asp
275 280 285
Arg?Phe?Asn?Glu?Arg?Lys?Asp?Thr?Val?Gln?Lys?Ile?Ala?Asn?Asn?Leu
290 295 300
Lys?Ala?Lys?Tyr?Gly?Glu?Asn?Ser?Val?Thr?Val?Asp?Met?Asn?Asp?Gln
305 310 315 320
Tyr?Tyr?Asn?Met?Arg?Glu?Lys?Ile?Glu?Pro?Val?Lys?Glu?Ile?Val?Asp
325 330 335
Ile?Ala?Tyr?Lys?Ala?Met?Lys?Asn?Leu?Asp?Ile?Glu?Pro?Val?Val?Lys
340 345 350
Pro?Ile?Arg?Gly?Gly?Thr?Asp?Gly?Ser?Gln?Leu?Ser?Tyr?Met?Gly?Leu
355 360 365
Pro?Cys?Pro?Asn?Ile?Phe?Thr?Gly?Gly?Glu?Asn?Phe?His?Gly?Lys?Tyr
370 375 380
Glu?Tyr?Ile?Ser?Ala?Asp?Asn?Met?Val?Lys?Ala?Ala?Asn?Val?Ile?Val
385 390 395 400
Glu?Ile?Val?Lys?Leu?Phe?Glu?Glu?Arg?Ala
405 410
<210>71
<211>2002
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1502)
<400>71
ttttattatg?gggcattttt?atcacagttg?gtaaatagcg?gatttgcccc?agctattttt 60
gaaaatagcg?aaaaaagacg?tatttataaa?ttgtcaacta?attatggtga?ctataaagtt 120
tatacgaagt?atgtatcgaa?gcaaagtaat?aaaggtcaaa?acagaatgtg?gcattttcca 180
ttcaatttgg?atgaagtaaa?caacattcaa?caagaccaat?ctatcaatat?gtttgctttt 240
atttgcgggg?ttgaagattt?agttaatagt?gagatagtga?ttttaagtag?agaggaattt 300
aatcagtgta?tcggtgtagg?ttttaaaaca?gaaaatcgac?gtgtgtcggt?taaatctgaa 360
aaaggtgcat?ggaaccattt?agtttatgga?actggtatag?aactaaaaga?aactccactt 420
aaggttatta?aaaacttgaa?taagagatta?agtgaatttt?caatggtctc?tacttgatga 480
taattaaaag?aggtgattag?gtg?aat?aat?gaa?tct?aat?ttt?cta?atg?tac?caa 533
Val?Asn?Asn?Glu?Ser?Asn?Phe?Leu?Met?Tyr?Gln
1 5 10
acc?gaa?aat?ggc?gat?acg?aaa?att?caa?gtc?cgt?tta?gaa?ggt?gaa?acc 581
Thr?Glu?Asn?Gly?Asp?Thr?Lys?Ile?Gln?Val?Arg?Leu?Glu?Gly?Glu?Thr
15 20 25
gtc?tgg?atg?acg?caa?aag?gca?atg?gca?gag?ctg?ttt?caa?aag?ggt?gtt 629
Val?Trp?Met?Thr?Gln?Lys?Ala?Met?Ala?Glu?Leu?Phe?Gln?Lys?Gly?Val
30 35 40
cca?aca?ata?aat?gag?cat?att?aaa?aat?ata?tat?gct?gaa?ggt?gag?tta 677
Pro?Thr?Ile?Asn?Glu?His?Ile?Lys?Asn?Ile?Tyr?Ala?Glu?Gly?Glu?Leu
45 50 55
aca?gag?gag?gca?act?att?cgg?aaa?aac?cga?ata?gtt?caa?gtt?gag?ggt 725
Thr?Glu?Glu?Ala?Thr?Ile?Arg?Lys?Asn?Arg?Ile?Val?Gln?Val?Glu?Gly
60 65 70 75
tct?cgt?gaa?gtt?gaa?cgt?gaa?gtg?acc?ttt?tac?aac?ctt?gaa?gtt?atc 773
Ser?Arg?Glu?Val?Glu?Arg?Glu?Val?Thr?Phe?Tyr?Asn?Leu?Glu?Val?Ile
80 85 90
att?gca?gtc?ggc?tat?cgt?gtt?cga?tct?cat?cgt?ggt?aca?caa?ttc?cgt 821
Ile?Ala?Val?Gly?Tyr?Arg?Val?Arg?Ser?His?Arg?Gly?Thr?Gln?Phe?Arg
95 100 105
caa?tgg?gca?aca?gag?cgt?tta?aat?gaa?tac?atg?gta?aaa?ggt?ttt?acg 869
Gln?Trp?Ala?Thr?Glu?Arg?Leu?Asn?Glu?Tyr?Met?Val?Lys?Gly?Phe?Thr
110 115 120
atg?gat?gat?gaa?cgc?tta?aaa?gaa?atg?cgg?aat?att?ggg?gca?gat?tat 917
Met?Asp?Asp?Glu?Arg?Leu?Lys?Glu?Met?Arg?Asn?Ile?Gly?Ala?Asp?Tyr
125 130 135
ttt?gat?gaa?tta?ttg?gaa?cgt?att?cgt?gat?att?cgt?gct?tcc?gaa?aga 965
Phe?Asp?Glu?Leu?Leu?Glu?Arg?Ile?Arg?Asp?Ile?Arg?Ala?Ser?Glu?Arg
140 145 150 155
cgt?ttt?tat?tac?aaa?ata?aca?gat?atc?tat?gct?aca?tct?att?gat?tac 1013
Arg?Phe?Tyr?Tyr?Lys?Ile?Thr?Asp?Ile?Tyr?Ala?Thr?Ser?Ile?Asp?Tyr
160 165 170
gat?ccg?aat?aca?cca?att?gca?aga?gaa?ttc?ttt?gca?acc?gtt?caa?aat 1061
Asp?Pro?Asn?Thr?Pro?Ile?Ala?Arg?Glu?Phe?Phe?Ala?Thr?Val?Gln?Asn
175 180 185
aaa?ctc?cat?ttt?gca?atc?cat?gga?cat?act?gct?tcg?gaa?ctg?ata?atg 1109
Lys?Leu?His?Phe?Ala?Ile?His?Gly?His?Thr?Ala?Ser?Glu?Leu?Ile?Met
190 195 200
aaa?cga?gcg?gat?gca?aca?aag?cca?aac?atg?ggc?tta?acg?agt?tgg?aaa 1157
Lys?Arg?Ala?Asp?Ala?Thr?Lys?Pro?Asn?Met?Gly?Leu?Thr?Ser?Trp?Lys
205 210 215
ggt?gac?aaa?gtg?cgt?aaa?cat?gat?gtc?act?gtg?gcg?aaa?aat?tat?tta 1205
Gly?Asp?Lys?Val?Arg?Lys?His?Asp?Val?Thr?Val?Ala?Lys?Asn?Tyr?Leu
220 225 230 235
aca?caa?gaa?gaa?ctc?agc?gat?tta?aac?cgt?atc?gtg?aca?atg?tat?tta 1253
Thr?Gln?Glu?Glu?Leu?Ser?Asp?Leu?Asn?Arg?Ile?Val?Thr?Met?Tyr?Leu
240 245 250
gac?tat?gca?gaa?aca?caa?gcg?aaa?aag?aaa?aag?ccg?atg?tac?atg?aag 1301
Asp?Tyr?Ala?Glu?Thr?Gln?Ala?Lys?Lys?Lys?Lys?Pro?Met?Tyr?Met?Lys
255 260 265
gat?tgg?gca?gaa?aaa?tta?gat?gca?ttt?tta?gag?ttt?aat?gaa?cat?gaa 1349
Asp?Trp?Ala?Glu?Lys?Leu?Asp?Ala?Phe?Leu?Glu?Phe?Asn?Glu?His?Glu
270 275 280
ata?tta?act?aac?gct?ggg?aaa?att?aaa?gcc?aaa?gta?gcg?gaa?caa?ttt 1397
Ile?Leu?Thr?Asn?Ala?Gly?Lys?Ile?Lys?Ala?Lys?Val?Ala?Glu?Gln?Phe
285 290 295
gca?aac?gaa?caa?tat?gaa?gtg?ttt?cat?caa?caa?cga?tta?gca?gaa?ccg 1445
Ala?Asn?Glu?Gln?Tyr?Glu?Val?Phe?His?Gln?Gln?Arg?Leu?Ala?Glu?Pro
300 305 310 315
aag?aaa?gat?gat?ttt?gat?caa?ttt?ttg?gaa?caa?agg?aag?cag?tta?gac 1493
Lys?Lys?Asp?Asp?Phe?Asp?Gln?Phe?Leu?Glu?Gln?Arg?Lys?Gln?Leu?Asp
320 325 330
agt?gaa?aaa?tagaaaggat?tttaggtgaa?tttcatggaa?gcatttaagc 1542
Ser?Glu?Lys
aatacaaatg?ggaaaatgga?aatcttagtg?acattgctga?cattactatg?ggccagtcgc 1602
caccaggtaa?ttcctataat?gatatcaagg?atggaatcgg?tttaatcaat?ggaccaacag 1662
aatttacgaa?taaataccca?gttgtaaaac?aatggacatc?taaacctaca?aaactttgta 1722
aagctgggga?tatattattg?tgtgtaagag?gaagttcaac?aggacggatg?aatatagctg 1782
atgatgaata?ttgcattggt?cgaggagtag?cttctattag?agctaaaaaa?gataaagctg 1842
aaacaagttt?tatttattac?acattaaatt?ataaagttaa?tcagttatta?caaaagactg 1902
cgggttctac?tttcccaaat?cttagtagta?atgaaataaa?agatatgatt?gtcggtattc 1962
cattatttgc?agaacaacaa?aaaatcgcct?ccatcctctc 2002
<210>72
<211>334
<212>PRT
＜213〉Bacillus licheniformis
<400>72
Val?Asn?Asn?Glu?Ser?Asn?Phe?Leu?Met?Tyr?Gln?Thr?Glu?Asn?Gly?Asp
1 5 10 15
Thr?Lys?Ile?Gln?Val?Arg?Leu?Glu?Gly?Glu?Thr?Val?Trp?Met?Thr?Gln
20 25 30
Lys?Ala?Met?Ala?Glu?Leu?Phe?Gln?Lys?Gly?Val?Pro?Thr?Ile?Asn?Glu
35 40 45
His?Ile?Lys?Asn?Ile?Tyr?Ala?Glu?Gly?Glu?Leu?Thr?Glu?Glu?Ala?Thr
50 55 60
Ile?Arg?Lys?Asn?Arg?Ile?Val?Gln?Val?Glu?Gly?Ser?Arg?Glu?Val?Glu
65 70 75 80
Arg?Glu?Val?Thr?Phe?Tyr?Asn?Leu?Glu?Val?Ile?Ile?Ala?Val?Gly?Tyr
85 90 95
Arg?Val?Arg?Ser?His?Arg?Gly?Thr?Gln?Phe?Arg?Gln?Trp?Ala?Thr?Glu
100 105 110
Arg?Leu?Asn?Glu?Tyr?Met?Val?Lys?Gly?Phe?Thr?Met?Asp?Asp?Glu?Arg
115 120 125
Leu?Lys?Glu?Met?Arg?Asn?Ile?Gly?Ala?Asp?Tyr?Phe?Asp?Glu?Leu?Leu
130 135 140
Glu?Arg?Ile?Arg?Asp?Ile?Arg?Ala?Ser?Glu?Arg?Arg?Phe?Tyr?Tyr?Lys
145 150 155 160
Ile?Thr?Asp?Ile?Tyr?Ala?Thr?Ser?Ile?Asp?Tyr?Asp?Pro?Asn?Thr?Pro
165 170 175
Ile?A1a?Arg?Glu?Phe?Phe?Ala?Thr?Val?Gln?Asn?Lys?Leu?His?Phe?Ala
180 185 190
Ile?His?Gly?His?Thr?Ala?Ser?Glu?Leu?Ile?Met?Lys?Arg?Ala?Asp?Ala
195 200 205
Thr?Lys?Pro?Asn?Met?Gly?Leu?Thr?Ser?Trp?Lys?Gly?Asp?Lys?Val?Arg
210 215 220
Lys?His?Asp?Val?Thr?Val?Ala?Lys?Asn?Tyr?Leu?Thr?Gln?Glu?Glu?Leu
225 230 235 240
Ser?Asp?Leu?Asn?Arg?Ile?Val?Thr?Met?Tyr?Leu?Asp?Tyr?Ala?Glu?Thr
245 250 255
Gln?Ala?Lys?Lys?Lys?Lys?Pro?Met?Tyr?Met?Lys?Asp?Trp?Ala?Glu?Lys
260 265 270
Leu?Asp?Ala?Phe?Leu?Glu?Phe?Asn?Glu?His?Glu?Ile?Leu?Thr?Asn?Ala
275 280 285
Gly?Lys?Ile?Lys?Ala?Lys?Val?Ala?Glu?Gln?Phe?Ala?Asn?Glu?Gln?Tyr
290 295 300
Glu?Val?Phe?His?Gln?Gln?Arg?Leu?Ala?Glu?Pro?Lys?Lys?Asp?Asp?Phe
305 310 315 320
Asp?Gln?Phe?Leu?Glu?Gln?Arg?Lys?Gln?Leu?Asp?Ser?Glu?Lys
325 330
<210>73
<211>2170
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1670)
<400>73
cgaatacacc?aattgcaaga?gaattctttg?caaccgttca?aaataaactc?cattttgcaa 60
tccatggaca?tactgcttcg?gaactgataa?tgaaacgagc?ggatgcaaca?aagccaaaca 120
tgggcttaac?gagttggaaa?ggtgacaaag?tgcgtaaaca?tgatgtcact?gtggcgaaaa 180
attatttaac?acaagaagaa?ctcagcgatt?taaaccgtat?cgtgacaatg?tatttagact 240
atgcagaaac?acaagcgaaa?aagaaaaagc?cgatgtacat?gaaggattgg?gcagaaaaat 300
tagatgcatt?tttagagttt?aatgaacatg?aaatattaac?taacgctggg?aaaattaaag 360
ccaaagtagc?ggaacaattt?gcaaacgaac?aatatgaagt?gtttcatcaa?caacgattag 420
cagaaccgaa?gaaagatgat?tttgatcaat?ttttggaaca?aaggaagcag?ttagacagtg 480
aaaaatagaa?aggattttag?gtg?aat?ttc?atg?gaa?gca?ttt?aag?caa?tac?aaa 533
Val?Asn?Phe?Met?Glu?Ala?Phe?Lys?Gln?Tyr?Lys
1 5 10
tgg?gaa?aat?gga?aat?ctt?agt?gac?att?gct?gac?att?act?atg?ggc?cag 581
Trp?Glu?Asn?Gly?Asn?Leu?Ser?Asp?Ile?Ala?Asp?Ile?Thr?Met?Gly?Gln
15 20 25
tcg?cca?cca?ggt?aat?tcc?tat?aat?gat?atc?aag?gat?gga?atc?ggt?tta 629
Ser?Pro?Pro?Gly?Asn?Ser?Tyr?Asn?Asp?Ile?Lys?Asp?Gly?Ile?Gly?Leu
30 35 40
atc?aat?gga?cca?aca?gaa?ttt?acg?aat?aaa?tac?cca?gtt?gta?aaa?caa 677
Ile?Asn?Gly?Pro?Thr?Glu?Phe?Thr?Asn?Lys?Tyr?Pro?Val?Val?Lys?Gln
45 50 55
tgg?aca?tct?aaa?cct?aca?aaa?ctt?tgt?aaa?gct?ggg?gat?ata?tta?ttg 725
Trp?Thr?Ser?Lys?Pro?Thr?Lys?Leu?Cys?Lys?Ala?Gly?Asp?Ile?Leu?Leu
60 65 70 75
tgt?gta?aga?gga?agt?tca?aca?gga?cgg?atg?aat?ata?gct?gat?gat?gaa 773
Cys?Val?Arg?Gly?Ser?Ser?Thr?Gly?Arg?Met?Asn?Ile?Ala?Asp?Asp?Glu
80 85 90
tat?tgc?att?ggt?cga?gga?gta?gct?tct?att?aga?gct?aaa?aaa?gat?aaa 821
Tyr?Cys?Ile?Gly?Arg?Gly?Val?Ala?Ser?Ile?Arg?Ala?Lys?Lys?Asp?Lys
95 100 105
gct?gaa?aca?agt?ttt?att?tat?tac?aca?tta?aat?tat?aaa?gtt?aat?cag 869
Ala?Glu?Thr?Ser?Phe?Ile?Tyr?Tyr?Thr?Leu?Asn?Tyr?Lys?Val?Asn?Gln
110 115 120
tta?tta?caa?aag?act?gcg?ggt?tct?act?ttc?cca?aat?ctt?agt?agt?aat 917
Leu?Leu?Gln?Lys?Thr?Ala?Gly?Ser?Thr?Phe?Pro?Asn?Leu?Ser?Ser?Asn
125 130 135
gaa?ata?aaa?gat?atg?att?gtc?ggt?att?cca?tta?ttt?gca?gaa?caa?caa 965
Glu?Ile?Lys?Asp?Met?Ile?Val?Gly?Ile?Pro?Leu?Phe?Ala?Glu?Gln?Gln
140 145 150 155
aaa?atc?gcc?tcc?atc?ctc?tct?acc?tgg?gat?aag?gca?att?gag?cta?aaa 1013
Lys?Ile?Ala?Ser?Ile?Leu?Ser?Thr?Trp?Asp?Lys?Ala?Ile?Glu?Leu?Lys
160 165 170
gaa?aag?cta?atc?gag?cag?aag?aaa?gag?cag?aaa?aaa?ggg?tta?atg?cag 1061
Glu?Lys?Leu?Ile?Glu?Gln?Lys?Lys?Glu?Gln?Lys?Lys?Gly?Leu?Met?Gln
175 180 185
aag?ttg?ctg?act?ggt?aaa?gtg?aga?ttg?cct?ggg?ttt?agt?gac?aaa?tgg 1109
Lys?Leu?Leu?Thr?Gly?Lys?Val?Arg?Leu?Pro?Gly?Phe?Ser?Asp?Lys?Trp
190 195 200
gaa?aag?aaa?aaa?atc?ggt?gaa?cta?tta?gaa?gaa?agc?aag?gta?att?gca 1157
Glu?Lys?Lys?Lys?Ile?Gly?Glu?Leu?Leu?Glu?Glu?Ser?Lys?Val?Ile?Ala
205 210 215
aaa?aat?ccg?caa?tta?gat?aaa?agg?att?act?gtg?cga?tta?aat?tta?aaa 1205
Lys?Asn?Pro?Gln?Leu?Asp?Lys?Arg?Ile?Thr?Val?Arg?Leu?Asn?Leu?Lys
220 225 230 235
ggt?gtt?tgt?aaa?aga?gaa?ata?tct?acg?gtg?gaa?aaa?gag?gga?gca?act 1253
Gly?Val?Cys?Lys?Arg?Glu?Ile?Ser?Thr?Val?Glu?Lys?Glu?Gly?Ala?Thr
240 245 250
aca?caa?tat?atc?aga?aaa?gaa?ggc?caa?ttt?att?tat?ggg?aaa?cag?aat 1301
Thr?Gln?Tyr?Ile?Arg?Lys?Glu?Gly?Gln?Phe?Ile?Tyr?Gly?Lys?Gln?Asn
255 260 265
ctt?cat?aag?ggg?gca?ttt?gga?ttg?att?cca?aaa?gag?tta?gat?gga?ttc 1349
Leu?His?Lys?Gly?Ala?Phe?Gly?Leu?Ile?Pro?Lys?Glu?Leu?Asp?Gly?Phe
270 275 280
cag?tct?tca?tct?gac?ata?cct?tgt?ttt?gat?ttt?aaa?gaa?ggt?gta?gat 1397
Gln?Ser?Ser?Ser?Asp?Ile?Pro?Cys?Phe?Asp?Phe?Lys?Glu?Gly?Val?Asp
285 290 295
ggt?ctc?tgg?ttt?tat?tat?tat?ttt?tca?aga?gaa?agc?ttc?tat?act?aat 1445
Gly?Leu?Trp?Phe?Tyr?Tyr?Tyr?Phe?Ser?Arg?Glu?Ser?Phe?Tyr?Thr?Asn
300 305 310 315
tta?gaa?aat?atc?tca?agt?ggt?aca?ggt?tct?aaa?cgg?atc?caa?cct?aag 1493
Leu?Glu?Asn?Ile?Ser?Ser?Gly?Thr?Gly?Ser?Lys?Arg?Ile?Gln?Pro?Lys
320 325 330
gaa?tta?tac?aag?tta?aca?att?aaa?ctc?ccg?tca?ctt?aga?gaa?caa?caa 1541
Glu?Leu?Tyr?Lys?Leu?Thr?Ile?Lys?Leu?Pro?Ser?Leu?Arg?Glu?Gln?Gln
335 340 345
aga?cag?tca?aaa?att?tta?gaa?tgt?agt?gat?aaa?gaa?att?tat?ttg?tta 1589
Arg?Gln?Ser?Lys?Ile?Leu?Glu?Cys?Ser?Asp?Lys?Glu?Ile?Tyr?Leu?Leu
350 355 360
gag?aaa?gaa?tta?gag?act?tat?aga?aaa?caa?aaa?caa?ggt?ctt?atg?caa 1637
Glu?Lys?Glu?Leu?Glu?Thr?Tyr?Arg?Lys?Gln?Lys?Gln?Gly?Leu?Met?Gln
365 370 375
ctt?ctt?cta?aca?ggg?aaa?gtc?cga?gtg?aag?gta?taattccttt?actccttttc 1690
Leu?Leu?Leu?Thr?Gly?Lys?Val?Arg?Val?Lys?Val
380 385 390
ctttattctc?aacaatggag?gtggagaaat?gtcgattccg?caaagttatg?atgaacgtta 1750
cattagtcaa?cagcctgcga?tcgaggtgtt?gcaaggctta?ggatatgagt?acatagaggc 1810
tgaacgagct?gaggcgatgc?gtgagaatct?ctatagcgtt?ttgctgaaaa?cggtattgga 1870
aaataaattg?aaggagttaa?attcctatca?gtataaaggt?gttacgtaca?agttcagtga 1930
agcgaacatc?caacaagcga?tgcgagattt?ggatgagcca?ctcacaaacg?gacttgtaaa 1990
ggcaaatgaa?agcatttatg?aaacgttgat?gttaggaaga?acttatacgg?aattacttcc 2050
agacggctcg?aaaaagtcat?ttaccattca?atatattgat?tgggagaatg?ttgaaaataa 2110
tgtttttcac?attgtagagg?aattttctgt?tgaacgtgta?gacggcaggg?gtacagttcg 2170
<210>74
<211>390
<212>PRT
＜213〉Bacillus licheniformis
<400>74
Val?Asn?Phe?Met?Glu?Ala?Phe?Lys?Gln?Tyr?Lys?Trp?Glu?Asn?Gly?Asn
1 5 10 15
Leu?Ser?Asp?Ile?Ala?Asp?Ile?Thr?Met?Gly?Gln?Ser?Pro?Pro?Gly?Asn
20 25 30
Ser?Tyr?Asn?Asp?Ile?Lys?Asp?Gly?Ile?Gly?Leu?Ile?Asn?Gly?Pro?Thr
35 40 45
Glu?Phe?Thr?Asn?Lys?Tyr?Pro?Val?Val?Lys?Gln?Trp?Thr?Ser?Lys?Pro
50 55 60
Thr?Lys?Leu?Cys?Lys?Ala?Gly?Asp?Ile?Leu?Leu?Cys?Val?Arg?Gly?Ser
65 70 75 80
Ser?Thr?Gly?Arg?Met?Asn?Ile?Ala?Asp?Asp?Glu?Tyr?Cys?Ile?Gly?Arg
85 90 95
Gly?Val?Ala?Ser?Ile?Arg?Ala?Lys?Lys?Asp?Lys?Ala?Glu?Thr?Ser?Phe
100 105 110
Ile?Tyr?Tyr?Thr?Leu?Asn?Tyr?Lys?Val?Asn?Gln?Leu?Leu?Gln?Lys?Thr
115 120 125
Ala?Gly?Ser?Thr?Phe?Pro?Asn?Leu?Ser?Ser?Asn?Glu?Ile?Lys?Asp?Met
130 135 140
Ile?Val?Gly?Ile?Pro?Leu?Phe?Ala?Glu?Gln?Gln?Lys?Ile?Ala?Ser?Ile
145 150 155 160
Leu?Ser?Thr?Trp?Asp?Lys?Ala?Ile?Glu?Leu?Lys?Glu?Lys?Leu?Ile?Glu
165 170 175
Gln?Lys?Lys?Glu?Gln?Lys?Lys?Gly?Leu?Met?Gln?Lys?Leu?Leu?Thr?Gly
180 185 190
Lys?Val?Arg?Leu?Pro?Gly?Phe?Ser?Asp?Lys?Trp?Glu?Lys?Lys?Lys?Ile
195 200 205
Gly?Glu?Leu?Leu?Glu?Glu?Ser?Lys?Val?Ile?Ala?Lys?Asn?Pro?Gln?Leu
210 215 220
Asp?Lys?Arg?Ile?Thr?Val?Arg?Leu?Asn?Leu?Lys?Gly?Val?Cys?Lys?Arg
225 230 235 240
Glu?Ile?Ser?Thr?Val?Glu?Lys?Glu?Gly?Ala?Thr?Thr?Gln?Tyr?Ile?Arg
245 250 255
Lys?Glu?Gly?Gln?Phe?Ile?Tyr?Gly?Lys?Gln?Asn?Leu?His?Lys?Gly?Ala
260 265 270
Phe?Gly?Leu?Ile?Pro?Lys?Glu?Leu?Asp?Gly?Phe?Gln?Ser?Ser?Ser?Asp
275 280 285
Ile?Pro?Cys?Phe?Asp?Phe?Lys?Glu?Gly?Val?Asp?Gly?Leu?Trp?Phe?Tyr
290 295 300
Tyr?Tyr?Phe?Ser?Arg?Glu?Ser?Phe?Tyr?Thr?Asn?Leu?Glu?Asn?Ile?Ser
305 310 315 320
Ser?Gly?Thr?Gly?Ser?Lys?Arg?Ile?Gln?Pro?Lys?Glu?Leu?Tyr?Lys?Leu
325 330 335
Thr?Ile?Lys?Leu?Pro?Ser?Leu?Arg?Glu?Gln?Gln?Arg?Gln?Ser?Lys?Ile
340 345 350
Leu?Glu?Cys?Ser?Asp?Lys?Glu?Ile?Tyr?Leu?Leu?Glu?Lys?Glu?Leu?Glu
355 360 365
Thr?Tyr?Arg?Lys?Gln?Lys?Gln?Gly?Leu?Met?Gln?Leu?Leu?Leu?Thr?Gly
370 375 380
Lys?Val?Arg?Val?Lys?Val
385 390
<210>75
<211>1528
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1118)
<220>
<221>misc_feature
<222>(1518)..(1518)
<223>n?is?a，c，g，or?t
<400>75
cggcggtgtt?gcagcattgc?tgaccgcgcc?gacttcagga?aaaaacttga?gaggacagct 60
taaagccaac?tgcggtaaat?gggaagacac?cgtcaaaagg?ctgaaaaacg?acggaatcgc 120
attaaaagag?caattcgtca?aaaccgccat?agaaggcgcg?gaaatcatta?aagacgtcgg 180
cagcgaactg?caaacgtcca?tccaggaatg?gcgcgaggaa?atcaaacctc?atcagcagga 240
cttaaaaaag?gaaatcgctg?agatcgaaga?aaagataaaa?cagctggaga?aaaccttaca 300
aaactaatat?actcagcgaa?aaaaataaac?cttaaaaaag?gggtgtgaat?ggaaaatttt 360
cacgctcctt?ttttaggtaa?aatcctgatt?tcacaccgta?aaactggatt?ttttaaaaaa 420
ttcgtaaatt?tacatcttta?ttaatcttta?ttttattggc?ataatagaaa?ttataagata 480
aagagaagca?ggtgaagtga?atg?aat?cga?gcg?gaa?gag?ccc?tat?acg?gtg?aaa 533
Met?Asn?Arg?Ala?Glu?Glu?Pro?Tyr?Thr?Val?Lys
1 5 10
gaa?gcc?ctt?tta?ttc?agt?caa?aga?atg?gca?cag?ctt?agc?aag?gcg?ctt 581
Glu?Ala?Leu?Leu?Phe?Ser?Gln?Arg?Met?Ala?Gln?Leu?Ser?Lys?Ala?Leu
15 20 25
tgg?aag?tcg?ata?gaa?aag?gat?tgg?cag?cag?tgg?att?aaa?cct?tat?gat 629
Trp?Lys?Ser?Ile?Glu?Lys?Asp?Trp?Gln?Gln?Trp?Ile?Lys?Pro?Tyr?Asp
30 35 40
ctg?aac?att?aac?gag?cac?cat?att?ttg?tgg?atc?gcc?tac?cag?ctg?aac 677
Leu?Asn?Ile?Asn?Glu?His?His?Ile?Leu?Trp?Ile?Ala?Tyr?Gln?Leu?Asn
45 50 55
gga?gca?tcc?att?tca?gaa?atc?gcc?aag?ttc?ggc?gtc?atg?cac?gta?tcc 725
Gly?Ala?Ser?Ile?Ser?Glu?Ile?Ala?Lys?Phe?Gly?Val?Met?His?Val?Ser
60 65 70 75
aca?gcg?ttt?aat?ttt?tca?aaa?aag?ctt?gag?gaa?aga?ggt?tac?ctg?gag 773
Thr?Ala?Phe?Asn?Phe?Ser?Lys?Lys?Leu?Glu?Glu?Arg?Gly?Tyr?Leu?Glu
80 85 90
ttt?tcc?aaa?aaa?ttg?aat?gac?aaa?cgc?aac?act?tac?att?cag?ctg?act 821
Phe?Ser?Lys?Lys?Leu?Asn?Asp?Lys?Arg?Asn?Thr?Tyr?Ile?Gln?Leu?Thr
95 100 105
ccg?aaa?ggt?gaa?gaa?gtt?ttc?ctt?aaa?atc?ctc?gag?tct?tac?gat?ccg 869
Pro?Lys?Gly?Glu?Glu?Val?Phe?Leu?Lys?Ile?Leu?Glu?Ser?Tyr?Asp?Pro
110 115 120
acc?cgg?aac?gcc?gtc?tta?aaa?ggc?gct?cag?ccc?ctt?cat?cag?ctg?tac 917
Thr?Arg?Asn?Ala?Val?Leu?Lys?Gly?Ala?Gln?Pro?Leu?His?Gln?Leu?Tyr
125 130 135
ggg?aag?ttt?cca?gaa?atc?gtc?gag?atg?atg?tcg?atc?atc?cgc?cat?att 965
Gly?Lys?Phe?Pro?Glu?Ile?Val?Glu?Met?Met?Ser?Ile?Ile?Arg?His?Ile
140 145 150 155
tac?ggc?gat?gat?ttc?atg?gaa?att?ttc?gaa?aaa?tcg?ttt?tca?aac?atc 1013
Tyr?Gly?Asp?Asp?Phe?Met?Glu?Ile?Phe?Glu?Lys?Ser?Phe?Ser?Asn?Ile
160 165 170
gaa?aat?gaa?ttt?acg?agc?gaa?gag?ggc?aaa?atg?aag?aaa?aaa?caa?gaa 1061
Glu?Asn?Glu?Phe?Thr?Ser?Glu?Glu?Gly?Lys?Met?Lys?Lys?Lys?Gln?Glu
175 180 185
gca?aaa?gaa?gcc?ggg?gaa?tcg?ata?gag?gtg?gac?aaa?ccg?ctt?gag?cct 1109
Ala?Lys?Glu?Ala?Gly?Glu?Ser?Ile?Glu?Val?Asp?Lys?Pro?Leu?Glu?Pro
190 195 200
ctt?aaa?aac?taagccagat?cgtactgcct?gataatgctg?atgaattcgc 1158
Leu?Lys?Asn
205
tcatcagcgg?tttgtaaaga?ccttgtctgt?gaagggcgaa?aatcgtttcg?tgcacttcaa 1218
gtttttcatt?taactgtccg?gcaaaaagcg?tgaggagatg?atcgaacatc?acaaatcctt 1278
gcgctttttg?tgcttcaagc?tcctttgaga?gctcttcgca?cagcttttcc?gtttcctttg 1338
cttcttcctc?tgataagccc?ttttcgatca?atattttata?gtacggatac?tcttccatat 1398
ccgcattgtt?caataacagt?ttgatgtaat?attccagccg?ctcgaggcgt?tgctccattg 1458
agttcatatt?tgtctcttct?ctccctttct?cttattttag?ccgatattcc?cttctgatan 1518
aagttcaaca 1528
<210>76
<211>206
<212>PRT
＜213〉Bacillus licheniformis
<400>76
Met?Asn?Arg?Ala?Glu?Glu?Pro?Tyr?Thr?Val?Lys?Glu?Ala?Leu?Leu?Phe
1 5 10 15
Ser?Gln?Arg?Met?Ala?Gln?Leu?Ser?Lys?Ala?Leu?Trp?Lys?Ser?Ile?Glu
20 25 30
Lys?Asp?Trp?Gln?Gln?Trp?Ile?Lys?Pro?Tyr?Asp?Leu?Asn?Ile?Asn?Glu
35 40 45
His?His?Ile?Leu?Trp?Ile?Ala?Tyr?Gln?Leu?Asn?Gly?Ala?Ser?Ile?Ser
50 55 60
Glu?Ile?Ala?Lys?Phe?Gly?Val?Met?His?Val?Ser?Thr?Ala?Phe?Asn?Phe
65 70 75 80
Ser?Lys?Lys?Leu?Glu?Glu?Arg?Gly?Tyr?Leu?Glu?Phe?Ser?Lys?Lys?Leu
85 90 95
Asn?Asp?Lys?Arg?Asn?Thr?Tyr?Ile?Gln?Leu?Thr?Pro?Lys?Gly?Glu?Glu
100 105 110
Val?Phe?Leu?Lys?Ile?Leu?Glu?Ser?Tyr?Asp?Pro?Thr?Arg?Asn?Ala?Val
115 120 125
Leu?Lys?Gly?Ala?Gln?Pro?Leu?His?Gln?Leu?Tyr?Gly?Lys?Phe?Pro?Glu
130 135 140
Ile?Val?Glu?Met?Met?Ser?Ile?Ile?Arg?His?Ile?Tyr?Gly?Asp?Asp?Phe
145 150 155 160
Met?Glu?Ile?Phe?Glu?Lys?Ser?Phe?Ser?Asn?Ile?Glu?Asn?Glu?Phe?Thr
165 170 175
Ser?Glu?Glu?Gly?Lys?Met?Lys?Lys?Lys?Gln?Glu?Ala?Lys?Glu?Ala?Gly
180 185 190
Glu?Ser?Ile?Glu?Val?Asp?Lys?Pro?Leu?Glu?Pro?Leu?Lys?Asn
195 200 205
<210>77
<211>2104
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1604)
<400>77
ggaaacgtca?gtccctgtcg?gttctgtcca?ggatacttta?tagacgcctt?cagtcagctt 60
gacgatatcg?gctttttgat?cgcgaaccca?gcgtccccca?accattccgc?tgtgaatgcg 120
gtaatcgatg?gtatggtcat?ttttaatgta?gatttcatat?tcccatccgt?tttcatacgt 180
atagatcata?tggcttccta?caaactcttt?tacatcttga?ttcatatgaa?ccgctccttt 240
atttgtttat?tgtaatcgaa?acatgttgat?atttacatat?ataattataa?aatgccgtca 300
aaaaagatgt?caaacgaaaa?tacttcctga?gagatttgca?caaataaaga?agattgttac 360
gattaatgtc?agaattttga?gttatcttag?gaaattatgc?caatatttag?aaaagtgatt 420
gtcaaaaaat?aagcgattct?gtaaaatgaa?aaacaaccca?taaaaaggaa?atgacatggg 480
aagaaaggaa?ggataaacga?ttg?aag?aca?aaa?att?gcg?tat?gag?gaa?gtt?gcg 533
Leu?Lys?Thr?Lys?Ile?Ala?Tyr?Glu?Glu?Val?Ala
1 5 10
gga?atg?ctt?aat?caa?tgg?tat?gtc?atg?atc?aag?cgt?cac?gaa?gta?tca 581
Gly?Met?Leu?Asn?Gln?Trp?Tyr?Val?Met?Ile?Lys?Arg?His?Glu?Val?Ser
15 20 25
caa?gcg?gtc?tcg?att?aaa?tgc?gac?att?gag?cac?cag?ctg?ccg?aat?atg 629
Gln?Ala?Val?Ser?Ile?Lys?Cys?Asp?Ile?Glu?His?Gln?Leu?Pro?Asn?Met
30 35 40
gaa?gaa?aat?caa?gat?ctg?ctt?ctt?tat?ttt?aat?ctt?tta?gac?tat?cgg 677
Glu?Glu?Asn?Gln?Asp?Leu?Leu?Leu?Tyr?Phe?Asn?Leu?Leu?Asp?Tyr?Arg
45 50 55
cac?aag?ctg?ctg?aca?gaa?gag?ttt?gcc?gct?tcc?aac?aaa?ctg?ttc?gag 725
His?Lys?Leu?Leu?Thr?Glu?Glu?Phe?Ala?Ala?Ser?Asn?Lys?Leu?Phe?Glu
60 65 70 75
gat?att?cag?gag?caa?aaa?gcc?gat?atg?caa?agc?aca?gat?gac?atg?att 773
Asp?Ile?Gln?Glu?Gln?Lys?Ala?Asp?Met?Gln?Ser?Thr?Asp?Asp?Met?Ile
80 85 90
gaa?tat?tat?tat?ttc?ttt?ttc?gct?ggc?atg?tac?gaa?ttt?cat?aag?aag 821
Glu?Tyr?Tyr?Tyr?Phe?Phe?Phe?Ala?Gly?Met?Tyr?Glu?Phe?His?Lys?Lys
95 100 105
gat?tat?aca?aat?gca?atc?aat?tat?tat?aaa?tta?gcc?gag?gaa?aag?ctc 869
Asp?Tyr?Thr?Asn?Ala?Ile?Asn?Tyr?Tyr?Lys?Leu?Ala?Glu?Glu?Lys?Leu
110 115 120
agg?aca?atc?ccc?gat?caa?atc?gaa?atc?gcc?gaa?ttc?cat?tac?aaa?ctg 917
Arg?Thr?Ile?Pro?Asp?Gln?Ile?Glu?Ile?Ala?Glu?Phe?His?Tyr?Lys?Leu
125 130 135
gct?atc?gcc?tac?tat?caa?atc?aaa?caa?aat?ttc?ctt?tcc?tta?aac?cat 965
Ala?Ile?Ala?Tyr?Tyr?Gln?Ile?Lys?Gln?Asn?Phe?Leu?Ser?Leu?Asn?His
140 145 150 155
gcg?aaa?aca?gct?cta?aaa?acc?ttc?aaa?gca?cat?gat?gat?tac?att?caa 1013
Ala?Lys?Thr?Ala?Leu?Lys?Thr?Phe?Lys?Ala?His?Asp?Asp?Tyr?Ile?Gln
160 165 170
aaa?gcg?atc?agc?aac?gat?atg?ctg?atc?ggg?gca?aat?aaa?ctc?gat?tta 1061
Lys?Ala?Ile?Ser?Asn?Asp?Met?Leu?Ile?Gly?Ala?Asn?Lys?Leu?Asp?Leu
175 180 185
ttt?cgt?ttt?gat?gaa?gcc?gaa?cag?cat?tac?aag?caa?gcc?ctt?aaa?gac 1109
Phe?Arg?Phe?Asp?Glu?Ala?Glu?Gln?His?Tyr?Lys?Gln?Ala?Leu?Lys?Asp
190 195 200
gcg?gca?ctg?atc?aaa?cat?cat?gtc?ctc?ctc?ggc?atg?gct?cac?cac?aac 1157
Ala?Ala?Leu?Ile?Lys?His?His?Val?Leu?Leu?Gly?Met?Ala?His?His?Asn
205 210 215
tta?ggg?ttg?agc?tat?gtc?aat?cgc?aac?ctc?ctc?aca?ttg?gct?gaa?cat 1205
Leu?Gly?Leu?Ser?Tyr?Val?Asn?Arg?Asn?Leu?Leu?Thr?Leu?Ala?Glu?His
220 225 230 235
cat?ttc?aaa?gaa?gcg?ctg?ctt?atc?aaa?gag?cat?gaa?gaa?tcg?gtt?tac 1253
Hi Phe?Lys?Glu?Ala?Leu?Leu?Ile?Lys?Glu?His?Glu?Glu?Ser?Val?Tyr
240 245 250
ggc?atc?cat?tcc?atg?ttt?gaa?ctg?aca?cat?gtg?ctg?tac?aaa?tca?aat 1301
Gly?Ile?His?Ser?Met?Phe?Glu?Leu?Thr?His?Val?Leu?Tyr?Lys?Ser?Asn
255 260 265
gtt?gtc?aaa?gaa?gca?cgc?aaa?ttg?tat?gaa?aaa?gga?ttt?ttc?cgt?gcg 1349
Val?Val?Lys?Glu?Ala?Arg?Lys?Leu?Tyr?Glu?Lys?Gly?Phe?Phe?Arg?Ala
270 275 280
gaa?aaa?gca?gga?gaa?agg?gaa?tat?ttg?tcg?aaa?ttt?aaa?ctt?att?cat 1397
Glu?Lys?Ala?Gly?Glu?Arg?Glu?Tyr?Leu?Ser?Lys?Phe?Lys?Leu?Ile?His
285 290 295
gct?ctg?tat?gat?gaa?cag?gat?cca?ctt?acg?gtt?gaa?cat?gct?tta?gaa 1445
Ala?Leu?Tyr?Asp?Glu?Gln?Asp?Pro?Leu?Thr?Val?Glu?His?Ala?Leu?Glu
300 305 3l0 315
tat?ctt?aaa?acg?atc?aat?ctc?tgg?acg?gat?gta?gcg?gaa?tta?aca?ttt 1493
Tyr?Leu?Lys?Thr?Ile?Asn?Leu?Trp?Thr?Asp?Val?Ala?Glu?Leu?Thr?Phe
320 325 330
gat?atc?gca?ctt?tac?tat?aaa?gaa?aat?gga?gat?gca?gac?aaa?gct?gcc 1541
Asp?Ile?Ala?Leu?Tyr?Tyr?Lys?Glu?Asn?Gly?Asp?Ala?Asp?Lys?Ala?Ala
335 340 345
gaa?tat?ttt?gaa?gaa?tct?cat?cat?gca?aga?gac?caa?att?ctt?aaa?aga 1589
Glu?Tyr?Phe?Glu?Glu?Ser?His?His?Ala?Arg?Asp?Gln?Ile?Leu?Lys?Arg
350 355 360
acg?gag?gag?tta?aag?tgaaaaagat?gattgccgtt?gcgttgactg?ccgtctttgc 1644
Thr?Glu?Glu?Leu?Lys
365
aagccttgtc?gttttaagtt?tttcgtctca?gccgaaggga?gatgccgagt?ttgccggccg 1704
agccattttt?cttgacgata?agccggctca?gctgatggcg?ggcagagcga?tctttctcga 1764
cagctttgac?ggaaattcgc?cagccgcctg?acaaaacacc?gcataaccct?gcttgtcacc 1824
ggacaagctc?tcttcgaaaa?gcgccgccag?cggcgctttt?tttatgaaag?caaatataac 1884
cggcgtccct?gcaaatcgct?tgcgaagatt?aaaaatatga?aacacatgac?aggaatggcg 1944
ctcaccggct?tttggtcgaa?tcctattgaa?aaatctgccc?gatttatagt?gcttgtcatt 2004
ctataaatcc?ttataatggt?cttaacgcca?atgtgaggca?tgccaataga?cagattgcag 2064
gggaaattct?atactgaaca?gcgcaattta?gcatgtaatc 2104
<210>78
<211>368
<212>PRT
＜213〉Bacillus licheniformis
<400>78
Leu?Lys?Thr?Lys?Ile?Ala?Tyr?Glu?Glu?Val?Ala?Gly?Met?Leu?Asn?Gln
1 5 10 15
Trp?Tyr?Val?Met?Ile?Lys?Arg?His?Glu?Val?Ser?Gln?Ala?Val?Ser?Ile
20 25 30
Lys?Cys?Asp?Ile?Glu?His?Gln?Leu?Pro?Asn?Met?Glu?Glu?Asn?Gln?Asp
35 40 45
Leu?Leu?Leu?Tyr?Phe?Asn?Leu?Leu?Asp?Tyr?Arg?His?Lys?Leu?Leu?Thr
50 55 60
Glu?Glu?Phe?Ala?Ala?Ser?Asn?Lys?Leu?Phe?Glu?Asp?Ile?Gln?Glu?Gln
65 70 75 80
Lys?Ala?Asp?Met?Gln?Ser?Thr?Asp?Asp?Met?Ile?Glu?Tyr?Tyr?Tyr?Phe
85 90 95
Phe?Phe?Ala?Gly?Met?Tyr?Glu?Phe?His?Lys?Lys?Asp?Tyr?Thr?Asn?Ala
100 105 110
Ile?Asn?Tyr?Tyr?Lys?Leu?Ala?Glu?Glu?Lys?Leu?Arg?Thr?Ile?Pro?Asp
115 120 125
Gln?Ile?Glu?Ile?Ala?Glu?Phe?His?Tyr?Lys?Leu?Ala?Ile?Ala?Tyr?Tyr
130 135 140
Gln?Ile?Lys?Gln?Asn?Phe?Leu?Ser?Leu?Asn?His?Ala?Lys?Thr?Ala?Leu
145 150 155 160
Lys?Thr?Phe?Lys?Ala?His?Asp?Asp?Tyr?Ile?Gln?Lys?Ala?Ile?Set?Asn
165 170 175
Asp?Met?Leu?Ile?Gly?Ala?Asn?Lys?Leu?Asp?Leu?Phe?Arg?Phe?Asp?Glu
180 185 190
Ala?Glu?Gln?His?Tyr?Lys?Gln?Ala?Leu?Lys?Asp?Ala?Ala?Leu?Ile?Lys
195 200 205
His?His?Val?Leu?Leu?Gly?Met?Ala?His?His?Asn?Leu?Gly?Leu?Ser?Tyr
210 215 220
Val?Asn?Arg?Asn?Leu?Leu?Thr?Leu?Ala?Glu?His?His?Phe?Lys?Glu?Ala
225 230 235 240
Leu?Leu?Ile?Lys?Glu?His?Glu?Glu?Ser?Val?Tyr?Gly?Ile?His?Ser?Met
245 250 255
Phe?Glu?Leu?Thr?His?Val?Leu?Tyr?Lys?Ser?Asn?Val?Val?Lys?Glu?Ala
260 265 270
Arg?Lys?Leu?Tyr?Glu?Lys?Gly?Phe?Phe?Arg?Ala?Glu?Lys?Ala?Gly?Glu
275 280 285
Arg?Glu?Tyr?Leu?Ser?Lys?Phe?Lys?Leu?Ile?His?Ala?Leu?Tyr?Asp?Glu
290 295 300
Gln?Asp?Pro?Leu?Thr?Val?Glu?His?Ala?Leu?Glu?Tyr?Leu?Lys?Thr?Ile
305 310 315 320
Asn?Leu?Trp?Thr?Asp?Val?Ala?Glu?Leu?Thr?Phe?Asp?Ile?Ala?Leu?Tyr
325 330 335
Tyr?Lys?Glu?Asn?Gly?Asp?Ala?Asp?Lys?Ala?Ala?Glu?Tyr?Phe?Glu?Glu
340 345 350
Ser?His?His?Ala?Arg?Asp?Gln?Ile?Leu?Lys?Arg?Thr?Glu?Glu?Leu?Lys
355 360 365
<210>79
<211>2005
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1505)
<400>79
acctcgattt?gctccgagtt?tgtcgcttca?atcattttat?cggtatcata?tatattaaaa 60
tccgcataca?aatgagcttt?cccctttttg?gccacgccta?cgtacaggca?gtcatccctg 120
aagttcgtct?ttcgaacagc?ctgcaaaaat?gtcccgtcgc?cgccgatgct?tgcgataata 180
ttggcttcat?tgtgatggtg?aacgacgcga?aatccgtgtt?cttcggcaag?ctgtttcagt 240
tttttgcact?gctcgtcggt?ttcttcgctt?tttttgtaaa?agaaataaat?gtttcggcgg 300
tctgtcacaa?tcattctccc?ctttgtctgt?taagtttaga?tttttctgcc?atctaacata 360
tatgttaaaa?tgacctgtga?cccttagttt?accatgtcca?aaaacttctt?gtatatgaag 420
cggcatcata?atgaactttt?tctcttcata?tacgtactaa?gacatgcagc?ttttttattc 480
aaaggaggag?aacagacaaa?atg?aat?gcg?aaa?aga?tgg?atc?gca?ctt?gtc?atc 533
Met?Asn?Ala?Lys?Arg?Trp?Ile?Ala?Leu?Val?Ile
1 5 10
gcg?ctg?ggc?gtt?ttt?gcg?tta?tct?gcg?gtg?atg?agc?ctt?ttg?ctt?gca 581
Ala?Leu?Gly?Val?Phe?Ala?Leu?Ser?Ala?Val?Met?Ser?Leu?Leu?Leu?Ala
15 20 25
gta?ttt?gac?acg?atg?ggg?aat?gac?gga?aag?atg?cag?ttt?ggc?ctc?aat 629
Val?Phe?Asp?Thr?Met?Gly?Asn?Asp?Gly?Lys?Met?Gln?Phe?Gly?Leu?Asn
30 35 40
gac?acc?cag?gaa?gaa?acg?gtg?ctt?gaa?caa?gga?aac?agc?tca?aga?aaa 677
Asp?Thr?Gln?Glu?Glu?Thr?Val?Leu?Glu?Gln?Gly?Asn?Ser?Ser?Arg?Lys
45 50 55
atc?gcc?gtt?ctt?gaa?gtg?aac?gga?acg?att?tcc?gac?aat?ggc?ggc?gct 725
Ile?Ala?Val?Leu?Glu?Val?Asn?Gly?Thr?Ile?Ser?Asp?Asn?Gly?Gly?Ala
60 65 70 75
tca?ggc?ctg?ttc?agc?tct?gaa?ggc?tac?aac?cac?aga?tcg?ttt?ttg?caa 773
Ser?Gly?Leu?Phe?Ser?Ser?Glu?Gly?Tyr?Asn?His?Arg?Ser?Phe?Leu?Gln
80 85 90
atg?ctt?gaa?aaa?gca?aaa?gac?gat?tct?gcc?gta?aag?ggc?att?gtt?ctg 821
Met?Leu?Glu?Lys?Ala?Lys?Asp?Asp?Ser?Ala?Val?Lys?Gly?Ile?Val?Leu
95 100 105
cgc?gtc?aat?tct?ccg?ggt?ggc?gga?gta?tac?gag?agc?gct?gag?ata?cat 869
Arg?Val?Asn?Ser?Pro?Gly?Gly?Gly?Val?Tyr?Glu?Ser?Ala?Glu?Ile?His
110 115 120
aag?aag?ctt?gaa?gag?atc?aaa?aaa?gat?acg?aaa?aaa?ccg?att?tac?gta 917
Lys?Lys?Leu?Glu?Glu?Ile?Lys?Lys?Asp?Thr?Lys?Lys?Pro?Ile?Tyr?Val
125 130 135
tca?atg?gga?tcg?atg?gct?gcg?tcc?ggc?ggc?tat?tac?att?tca?acg?cct 965
Ser?Met?Gly?Ser?Met?Ala?Ala?Ser?Gly?Gly?Tyr?Tyr?Ile?Ser?Thr?Pro
140 145 150 155
gcc?gac?aaa?atc?ttt?gca?gcg?cct?gac?acg?ctg?acg?ggt?tca?ctc?ggg 1013
Ala?Asp?Lys?Ile?Phe?Ala?Ala?Pro?Asp?Thr?Leu?Thr?Gly?Ser?Leu?Gly
160 165 170
gtc?atc?atg?gaa?agc?ctt?aac?tac?agc?aag?ctt?gca?gag?aag?ctt?gga 1061
Val?Ile?Met?Glu?Ser?Leu?Asn?Tyr?Ser?Lys?Leu?Ala?Glu?Lys?Leu?Gly
175 180 185
tta?aaa?acg?gag?acg?att?aaa?agc?ggt?gag?ttt?aaa?gat?atc?atg?tca 1109
Leu?Lys?Thr?Glu?Thr?Ile?Lys?Ser?Gly?Glu?Phe?Lys?Asp?Ile?Met?Ser
190 195 200
ccg?acg?cgc?gat?atg?acg?aaa?aaa?gaa?aga?gaa?atc?atg?cag?tca?atg 1157
Pro?Thr?Arg?Asp?Met?Thr?Lys?Lys?Glu?Arg?Glu?Ile?Met?Gln?Ser?Met
205 210 215
gtc?gat?gac?gcg?tac?gaa?ggg?ttt?gta?gat?gtt?att?gcc?gaa?ggg?cgc 1205
Val?Asp?Asp?Ala?Tyr?Glu?Gly?Phe?Val?Asp?Val?Ile?Ala?Glu?Gly?Arg
220 225 230 235
ggc?atg?tcc?gaa?aat?gac?gtg?aaa?aaa?att?gcc?gac?gga?cgt?gtc?tat 1253
Gly?Met?Ser?Glu?Asn?Asp?Val?Lys?Lys?Ile?Ala?Asp?Gly?Arg?Val?Tyr
240 245 250
gac?ggc?cgc?cag?gcg?aaa?cag?aac?cat?ctc?att?gat?gag?ctc?ggc?tat 1301
Asp?Gly?Arg?Gln?Ala?Lys?Gln?Asn?His?Leu?Ile?Asp?Glu?Leu?Gly?Tyr
255 260 265
tat?gaa?gat?gcg?gtc?aaa?gcg?atg?aaa?aag?gac?cac?aaa?aac?ctt?gcc 1349
Tyr?Glu?Asp?Ala?Val?Lys?Ala?Met?Lys?Lys?Asp?His?Lys?Asn?Leu?Ala
270 275 280
ggc?gca?tct?gtt?gtc?agc?tat?gaa?gaa?tca?gcc?ggc?ctt?gcc?tcg?ctg 1397
Gly?Ala?Ser?Val?Val?Ser?Tyr?Glu?Glu?Ser?Ala?Gly?Leu?Ala?Ser?Leu
285 290 295
ttt?tca?atg?act?gca?aat?aag?atg?ttt?aaa?agc?gaa?gct?gat?ttc?tta 1445
Phe?Ser?Met?Thr?Ala?Asn?Lys?Met?Phe?Lys?Ser?Glu?Ala?Asp?Phe?Leu
300 305 310 315
aac?att?aaa?gaa?gcg?att?tct?caa?tcc?ggt?gca?ccg?aga?ctc?atg?tat 1493
Asn?Ile?Lys?Glu?Ala?Ile?Ser?Gln?Ser?Gly?Ala?Pro?Arg?Leu?Met?Tyr
320 325 330
tta?tat?gcc?aaa?taggagggga?ccgaaaaaat?ggatgtgact?tatgatggaa 1545
Leu?Tyr?Ala?Lys
335
aggatcataa?tgaattatcg?ccgaatgtcg?gcgtttccag?ggaagaatcg?gccccgcatg 1605
tagagcatgc?ctatgccggt?ttttggatca?ggctgtttgc?ttttctgctt?gacggggttg 1665
ttatcggcag?catcaacaac?ttagcggttt?cacccgtttt?cagcctgctg?aacctcccta 1725
aagaatcagg?gtttttcacc?ttttcgcttt?attcttttgt?gacggcggca?gtattttttg 1785
cttactttgt?cctgatgacg?aagtatttcg?gacagacgtt?gggcaagatg?gtattcgggc 1845
tgaaggtcgt?gtcgcttgtc?ccggaaaaag?ggctgacatg?ggatgtggtc?ctgtttaggg 1905
agtttatcgg?ccgctatatc?aacagtttat?atattacgta?tctcgtagca?gcgttttcgc 1965
ctaagaaaca?agggattcac?gatttctttg?cggacccctg 2005
<210>80
<211>335
<212>PRT
＜213〉Bacillus licheniformis
<400>80
Met?Asn?Ala?Lys?Arg?Trp?Il?e?Ala?Leu?Val?Ile?Ala?Leu?Gly?Val?Phe
1 5 10 15
Ala?Leu?Ser?Ala?Val?Met?Ser?Leu?Leu?Leu?Ala?Val?Phe?Asp?Thr?Met
20 25 30
Gly?Asn?Asp?Gly?Lys?Met?Gln?Phe?Gly?Leu?Asn?Asp?Thr?Gln?Glu?Glu
35 40 45
Thr?Val?Leu?Glu?Gln?Gly?Asn?Ser?Ser?Arg?Lys?Ile?Ala?Val?Leu?Glu
50 55 60
Val?Asn?Gly?Thr?Ile?Ser?Asp?Asn?Gly?Gly?Ala?Ser?Gly?Leu?Phe?Ser
65 70 75 80
Ser?Glu?Gly?Tyr?Asn?His?Arg?Ser?Phe?Leu?Gln?Met?Leu?Glu?Lys?Ala
85 90 95
Lys?Asp?Asp?Ser?Ala?Val?Lys?Gly?Ile?Val?Leu?Arg?Val?Asn?Ser?Pro
100 105 110
Gly?Gly?Gly?Val?Tyr?Glu?Ser?Ala?Glu?Ile?His?Lys?Lys?Leu?Glu?Glu
115 120 125
Ile?Lys?Lys?Asp?Thr?Lys?Lys?Pro?Ile?Tyr?Val?Ser?Met?Gly?Ser?Met
130 135 140
Ala?Ala?Ser?Gly?Gly?Tyr?Tyr?Ile?Ser?Thr?Pro?Ala?Asp?Lys?Ile?Phe
145 150 155 160
Ala?Ala?Pro?Asp?Thr?Leu?Thr?Gly?Ser?Leu?Gly?Val?Ile?Met?Glu?Ser
165 170 175
Leu?Asn?Tyr?Ser?Lys?Leu?Ala?Glu?Lys?Leu?Gly?Leu?Lys?Thr?Glu?Thr
180 185 190
Ile?Lys?Ser?Gly?Glu?Phe?Lys?Asp?Ile?Met?Ser?Pro?Thr?Arg?Asp?Met
195 200 205
Thr?Lys?Lys?Glu?Arg?Glu?Ile?Met?Gln?Ser?Met?Val?Asp?Asp?Ala?Tyr
210 215 220
Glu?Gly?Phe?Val?Asp?Val?Ile?Ala?Glu?Gly?Arg?Gly?Met?Ser?Glu?Asn
225 230 235 240
Asp?Val?Lys?Lys?Ile?Ala?Asp?Gly?Arg?Val?Tyr?Asp?Gly?Arg?Gln?Ala
245 250 255
Lys?Gln?Asn?His?Leu?Ile?Asp?Glu?Leu?Gly?Tyr?Tyr?Glu?Asp?Ala?Val
260 265 270
Lys?Ala?Met?Lys?Lys?Asp?His?Lys?Asn?Leu?Ala?Gly?Ala?Ser?Val?Val
275 280 285
Ser?Tyr?Glu?Glu?Ser?Ala?Gly?Leu?Ala?Ser?Leu?Phe?Ser?Met?Thr?Ala
290 295 300
Asn?Lys?Met?Phe?Lys?Ser?Glu?Ala?Asp?Phe?Leu?Asn?Ile?Lys?Glu?Ala
305 310 315 320
Ile?Ser?Gln?Ser?Gly?Ala?Pro?Arg?Leu?Met?Tyr?Leu?Tyr?Ala?Lys
325 330 335
<210>81
<211>1326
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(869)
<400>81
tgcgttgact?gccgtctttg?caagccttgt?cgttttaagt?ttttcgtctc?agccgaaggg 60
agatgccgag?tttgccggcc?gagccatttt?tcttgacgat?aagccggctc?agctgatggc 120
gggcagagcg?atctttctcg?acagctttga?cggaaattcg?ccagccgcct?gacaaaacac 180
cgcataaccc?tgcttgtcac?cggacaagct?ctcttcgaaa?agcgccgcca?gcggcgcttt 240
ttttatgaaa?gcaaatataa?ccggcgtccc?tgcaaatcgc?ttgcgaagat?taaaaatatg 300
aaacacatga?caggaatggc?gctcaccggc?ttttggtcga?atcctattga?aaaatctgcc 360
cgatttatag?tgcttgtcat?tctataaatc?cttataatgg?tcttaacgcc?aatgtgaggc 420
atgccaatag?acagattgca?ggggaaattc?tatactgaac?agcgcaattt?agcatgtaat 480
catttttagg?agggagcagc?atg?gaa?gtc?ttt?gaa?gaa?tat?tta?gcg?gga?att 533
Met?Glu?Val?Phe?Glu?Glu?Tyr?Leu?Ala?Gly?Ile
1 5 10
gat?cat?ccg?gaa?cat?cgt?gcc?cgg?atg?gaa?gaa?gta?ttg?ggt?tgg?gta 581
Asp?His?Pro?Glu?His?Arg?Ala?Arg?Met?Glu?Glu?Val?Leu?Gly?Trp?Val
15 20 25
gcg?gag?aca?ttt?cca?aat?tta?acg?ccg?aaa?atc?gct?tgg?aat?cag?ccg 629
Ala?Glu?Thr?Phe?Pro?Asn?Leu?Thr?Pro?Lys?Ile?Ala?Trp?Asn?Gln?Pro
30 35 40
atg?ttt?act?gat?cac?ggc?aca?ttt?att?atc?ggc?ttt?agc?gtc?gcg?aaa 677
Met?Phe?Thr?Asp?His?Gly?Thr?Phe?Ile?Ile?Gly?Phe?Ser?Val?Ala?Lys
45 50 55
aat?cat?ttg?gct?gtc?gcc?cct?gaa?agc?gct?ggg?atc?gat?cgt?ttt?tct 725
Asn?His?Leu?Ala?Val?Ala?Pro?Glu?Ser?Ala?Gly?Ile?Asp?Arg?Phe?Ser
60 65 70 75
gag?gaa?att?gtg?cag?gcc?ggc?tat?gat?cat?acg?aag?cag?ctt?gtc?cgt 773
Glu?Glu?Ile?Val?Gln?Ala?Gly?Tyr?Asp?His?Thr?Lys?Gln?Leu?Val?Arg
80 85 90
ttc?cgc?tgg?gat?cgt?ccg?gtg?gat?ttt?tca?ttg?ctt?gag?aaa?atg?atc 821
Phe?Arg?Trp?Asp?Arg?Pro?Val?Asp?Phe?Ser?Leu?Leu?Glu?Lys?Met?Ile
95 100 105
gag?ttt?aat?att?gcg?gat?aag?gcg?gac?tgc?tcg?act?ttt?tgg?cgg?aaa 869
Glu?Phe?Asn?Ile?Ala?Asp?Lys?Ala?Asp?Cys?Ser?Thr?Phe?Trp?Arg?Lys
110 115 120
taaaaaagcg?caaaaataag?gggcatcctc?tcatgaaggc?gagagagcgc?cccgaaagaa 929
aaacgctgtt?tatctaaaac?tccaaggttt?cgcgcatgta?cgcggcaaac?ctacccgtta 989
tgatgcttat?acatccaaag?caagccggat?ttcagcaggc?gaggcactct?gccgatcaac 1049
ggccgttcag?caactaaacc?gaaaccggct?tttttgccga?gcgacccgag?gacgcctttc 1109
agcttgaatt?tcggcattgt?ttcaggaaga?ggttcgcctt?tccagcgcag?ttcaagcact 1169
tggacgattt?gttccgcctg?ggcttcggcc?agctgtgcgc?tcggcgcgtg?aggcaggctt 1229
gcacagtcgc?cgacaacata?aacgtgttcg?tctcccggca?ggttatggtg?cggcgtcagg 1289
acgacgcgcc?cttgcggatc?tttttccaca?tcgagat 1326
<210>82
<211>123
<212>PRT
＜213〉Bacillus licheniformis
<400>82
Met?Glu?Val?Phe?Glu?Glu?Tyr?Leu?Ala?Gly?Ile?Asp?His?Pro?Glu?His
1 5 10 15
Arg?Ala?Arg?Met?Glu?Glu?Val?Leu?Gly?Trp?Val?Ala?Glu?Thr?Phe?Pro
20 25 30
Asn?Leu?Thr?Pro?Lys?Ile?Ala?Trp?Asn?Gln?Pro?Met?Phe?Thr?Asp?His
35 40 45
Gly?Thr?Phe?Ile?Ile?Gly?Phe?Ser?Val?Ala?Lys?Asn?His?Leu?Ala?Val
50 55 60
Ala?Pro?Glu?Ser?Ala?Gly?Ile?Asp?Arg?Phe?Ser?Glu?Glu?Ile?Val?Gln
65 70 75 80
Ala?Gly?Tyr?Asp?His?Thr?Lys?Gln?Leu?Val?Arg?Phe?Arg?Trp?Asp?Arg
85 90 95
Pro?Val?Asp?Phe?Ser?Leu?Leu?Glu?Lys?Met?Ile?Glu?Phe?Asn?Ile?Ala
100 105 110
Asp?Lys?Ala?Asp?Cys?Ser?Thr?Phe?Trp?Arg?Lys
115 120
<210>83
<211>1891
<212>DNA
＜213〉Bacillus licheniformis
<220>
<221>CDS
<222>(501)..(1391)
<400>83
gtacaatgag?aaggatgacg?gcttggtcga?tggaatattt?tttgccgagt?tccggatgcc 60
ccatcaaatc?ttttatatcg?cgtttgatcc?agttttggat?ggctgtcgat?tttaaggatg 120
tcagttcgca?caaatttcca?agttcaacaa?tttcatttgt?ggaaagtccg?tgttccgttt 180
tttgtttttt?ccgttcgtac?cgggcaatga?attcggggat?gtccaaattg?tcttccggct 240
catcgggcgc?caaatcggcc?gatctcaaaa?gaatcgctag?cggactgaca?tccagctgcc 300
cttttaaagc?gtacatcagt?ttggccattt?ccaggcgagt?cagtttaaaa?ggtttcatca 360
ttccacctca?ttatgtatta?aaatgaaata?gcaaacaata?taaacattat?cttacgtctt 420
atcacttgca?ttttcaattc?tatgccgtat?aatttttatt?atacgagttc?atttgatctt 480
attataacat?ggaggtaatc?atg?gga?aaa?aga?atc?ttt?ctc?ttt?ctt?tta?tcg 533
Met?Gly?Lys?Arg?Ile?Phe?Leu?Phe?Leu?Leu?Ser
1 5 10
aac?atc?tta?gtc?att?acg?acg?atc?ggt?atc?gtg?ctt?tcg?att?atc?ggt 581
Asn?Ile?Leu?Val?Ile?Thr?Thr?Ile?Gly?Ile?Val?Leu?Ser?Ile?Ile?Gly
15 20 25
tcg?ctg?aca?ggg?gta?ggc?acg?tat?ttc?acg?gct?aac?ggc?ggc?atc?gat 629
Ser?Leu?Thr?Gly?Val?Gly?Thr?Tyr?Phe?Thr?Ala?Asn?Gly?Gly?Ile?Asp
30 35 40
att?gtt?gcg?ctt?ctc?gta?ttt?agt?gca?gtt?gtc?ggt?ttt?gtc?ggc?tcc 677
Ile?Val?Ala?Leu?Leu?Val?Phe?Ser?Ala?Val?Val?Gly?Phe?Val?Gly?Ser
45 50 55
ttt?atg?tcg?ctc?ttg?atg?tca?aga?tgg?atg?gcg?aaa?atg?gcg?atg?ggc 725
Phe?Met?Ser?Leu?Leu?Met?Ser?Arg?Trp?Met?Ala?Lys?Met?Ala?Met?Gly
60 65 70 75
gtt?cag?gtg?ctg?aat?cct?gat?aag?caa?acg?ctc?agc?tat?gaa?gaa?cag 773
Val?Gln?Val?Leu?Asn?Pro?Asp?Lys?Gln?Thr?Leu?Ser?Tyr?Glu?Glu?Gln
80 85 90
cag?ctc?gtt?gac?cgg?gta?tat?aag?ctg?tcc?cgc?gct?gcc?gga?ctg?aca 821
Gln?Leu?Val?Asp?Arg?Val?Tyr?Lys?Leu?Ser?Arg?Ala?Ala?Gly?Leu?Thr
95 100 105
aag?atg?cct?gaa?gtc?ggc?atc?tac?aat tca?cgc?gaa?gtg?aat?gcc?ttt 869
Lys?Met?Pro?Glu?Val?Gly?Ile?Tyr?Asn?Ser?Arg?Glu?Val?Asn?Ala?Phe
110 115 120
gcg?acc?ggg?ccg?tct?aaa?aac?cgc?tcg?ctt?gtc?gct?gta?tcg?acg?ggc 917
Ala?Thr?Gly?Pro?Ser?Lys?Asn?Arg?Ser?Leu?Val?Ala?Val?Ser?Thr?Gly
125 130 135
ctt?ctt?cag?gaa?atg?gat?gac?gac?gca?gtg?gaa?ggc?gta?ctt?gcc?cac 965
Leu?Leu?Gln?Glu?Met?Asp?Asp?Asp?Ala?Val?Glu?Gly?Val?Leu?Ala?His
140 145 150 155
gaa?gtg?gcg?cat?att?gca?aac?ggc?gat?atg?gtg?acg?atg?acg?ctc?ctt 1013
Glu?Val?Ala?His?Ile?Ala?Asn?Gly?Asp?Met?Val?Thr?Met?Thr?Leu?Leu
160 165 170
caa?gga?atc?gtc?aat?acg?ttc?gtt?gtt?ttc?ttc?gca?agg?att?gcg?gca 1061
Gln?Gly?Ile?Val?Asn?Thr?Phe?Val?Val?Phe?Phe?Ala?Arg?Ile?Ala?Ala
175 180 185
tgg?gct?gtt?tca?cgc?gtt?gtc?cgt?gaa?gag?ctg?gcg?ccg?atc?gtt?cac 1109
Trp?Ala?Val?Ser?Arg?Val?Val?Arg?Glu?Glu?Leu?Ala?Pro?Ile?Val?His
190 195 200
ttt?atc?gcg?gtc?atc?gta?ttc?caa?att?gtg?ttc?tct?att?ctc?gga?agc 1157
Phe?Ile?Ala?Val?Ile?Val?Phe?Gln?Ile?Val?Phe?Ser?Ile?Leu?Gly?Ser
205 210 215
ctt?gtc?gtg?ttt?gcg?tac?tcc?cgc?cac?cgc?gaa?tat?cat?gcg?gat?aga 1205
Leu?Val?Val?Phe?Ala?Tyr?Ser?Arg?His?Arg?Glu?Tyr?His?Ala?Asp?Arg
220 225 230 235
gga?gga?gca?gac?ctt?gca?ggc?aag?gat?aag?atg?att?cac?gcg?ctc?cgc 1253
Gly?Gly?Ala?Asp?Leu?Ala?Gly?Lys?Asp?Lys?Met?Ile?His?Ala?Leu?Arg
240 245 250
tcg?ctt?gag?cag?tac?act?tca?aga?gtc?aaa?gaa?gaa?cag?gca?tct?gtt 1301
Ser?Leu?Glu?Gln?Tyr?Thr?Ser?Arg?Val?Lys?Glu?Glu?Gln?Ala?Ser?Val
255 260 265
gca?act?tta?aaa?atc?aac?ggg?aaa?aag?cat?aca?tcg?ctt?ttc?tca?aca 1349
Ala?Thr?Leu?Lys?Ile?Asn?Gly?Lys?Lys?His?Thr?Ser?Leu?Phe?Ser?Thr
270 275 280
cac?cct?gat?tta?agc?gac?aga?atc?cgc?cgt?ctg?gaa?gca?aaa 1391
His?Pro?Asp?Leu?Ser?Asp?Arg?Ile?Arg?Arg?Leu?Glu?Ala?Lys
285 290 295
taataaagat?gcaaaccccc?tttttcaagg?gggtttgctt?tttaaattta?aaaatcgaaa 1451
agcggctgta?cataatagca?tgtttccttt?aaaatggaac?acgttataca?caagaagcaa 1511
cttttgaata?ttgaaaagaa?ggaacgatta?tgaaggtttt?attgagaaaa?ctactgcgcg 1571
tgctgtctcc?tgtacagctg?atcgcgctct?attatttttt?agcggtgaca?gtttcggttg 1631
ttttattgag?tctgcccgtt?gcacacaaaa?ataatgttga?atggtctttt?atcgacgccc 1691
tttttaccgc?ggtgagcgca?gtgagtgtaa?ccggtttgac?ggttgttgat?acagccgata 1751
cattcagcac?ggccggaatt?tggattttgg?cgtttgttct?gcaatttggc?gggatcggcg 1811
tgatggcttt?gggtacattc?gtctggctga?ttttcggcaa?gcggatcggt?ttgaaagagc 1871
gccgtctgat?tatgaccgat 1891
<210>84
<211>297
<212>PRT
＜213〉Bacillus licheniformis
<400>84
Met?Gly?Lys?Arg?Ile?Phe?Leu?Phe?Leu?Leu?Ser?Asn?Ile?Leu?Val?Ile
1 5 10 15
Thr?Thr?Ile?Gly?Ile?Val?Leu?Ser?Ile?Ile?Gly?Ser?Leu?Thr?Gly?Val
20 25 30
Gly?Thr?Tyr?Phe?Thr?Ala?Asn?Gly?Gly?Ile?Asp?Ile?Val?Ala?Leu?Leu
35 40 45
Val?Phe?Ser?Ala?Val?Val?Gly?Phe?Val?Gly?Ser?Phe?Met?Ser?Leu?Leu
50 55 60
Met?Ser?Arg?Trp?Met?Ala?Lys?Met?Ala?Met?Gly?Val?Gln?Val?Leu?Asn
65 70 75 80
Pro?Asp?Lys?Gln?Thr?Leu?Ser?Tyr?Glu?Glu?Gln?Gln?Leu?Val?Asp?Arg
85 90 95
Val?Tyr?Lys?Leu?Ser?Arg?Ala?Ala?Gly?Leu?Thr?Lys?Met?Pro?Glu?Val
100 105 110
Gly?Ile?Tyr?Asn?Ser?Arg?Glu?Val?Asn?Ala?Phe?Ala?Thr?Gly?Pro?Ser
115 120 125
Lys?Asn?Arg?Ser?Leu?Val?Ala?Val?Ser?Thr?Gly?Leu?Leu?Gln?Glu?Met
130 135 140
Asp?Asp?Asp?Ala?Val?Glu?Gly?Val?Leu?Ala?His?Glu?Val?Ala?His?Ile
145 150 155 160
Ala?Asn?Gly?Asp?Met?Val?Thr?Met?Thr?Leu?Leu?Gln?Gly?Ile?Val?Asn
165 170 175
Thr?Phe?Val?Val?Phe?Phe?Ala?Arg?Ile?Ala?Ala?Trp?Ala?Val?Ser?Arg
180 185 190
Val?Val?Arg?Glu?Glu?Leu?Ala?Pro?Ile?Val?His?Phe?Ile?Ala?Val?Ile
195 200 205
Val?Phe?Gln?Ile?Val?Phe?Ser?Ile?Leu?Gly?Ser?Leu?Val?Val?Phe?Ala
210 215 220
Tyr?Ser?Arg?His?Arg?Glu?Tyr?His?Ala?Asp?Arg?Gly?Gly?Ala?Asp?Leu
225 230 235 240
Ala?Gly?Lys?Asp?Lys?Met?Ile?His?Ala?Leu?Arg?Ser?Leu?Glu?Gln?Tyr
245 250 255
Thr?Ser?Arg?Val?Lys?Glu?Glu?Gln?Ala?Ser?Val?Ala?Thr?Leu?Lys?Ile
260 265 270
Asn?Gly?Lys?Lys?His?Thr?Ser?Leu?Phe?Ser?Thr?His?Pro?Asp?Leu?Ser
275 280 285
Asp?Arg?Ile?Arg?Arg?Leu?Glu?Ala?Lys
290 295
Claims (21)
1. Bacillus licheniformis mutant host cell, it is derived from parental generation Bacillus licheniformis host cell, wherein said mutant host cell has one or more gene to undergo mutation, one or more has proteolytic activity and the polypeptide identical with one or more polypeptide at least 80% shown in the SEQ ID NO:2 to 84 described genes encoding, described one or more of wherein said mutant host cell expression has the polypeptide of proteolytic activity, lacks at least 5% than the parental generation host cell of cultivating under condition of equivalent.
2. host cell according to claim 1, its partially or completely disappearance by one or more gene is suddenlyd change, and described one or more of described genes encoding has the polypeptide of proteolytic activity.
3. according to the arbitrary described host cell of claim 1-2, it suddenlys change in the gene of two or more coding two or multiple polypeptide with proteolytic activity.
4. according to the arbitrary described host cell of claim 1-3, it comprises the heterologous gene of one or more one or more heterologous polypeptide of encoding.
5. host cell according to claim 4, wherein said heterologous gene exists with at least two copies.
6. according to claim 4 or 5 described host cells, wherein said heterologous gene is stabilized the genome that is incorporated into described cell.
7. according to the arbitrary described host cell of claim 4-6, wherein said heterologous gene is integrated into the genome of described cell, and does not stay any antibiotic resistance markers gene at integration site.
8. according to the arbitrary described host cell of claim 4-7, wherein said heterologous gene is transcribed from allogeneic promoter or manual activation.
9. according to the arbitrary described host cell of claim 4-8, wherein said heterologous gene is comprised in operon, in the preferred polycistronic operon.
10. according to the arbitrary described host cell of claim 4-9, wherein said heterologous polypeptide is antimicrobial peptide or fusogenic peptide, and described fusogenic peptide comprises the peptide moiety that has antimicrobial acivity when for natural form.
11. according to the arbitrary described host cell of claim 4-9, wherein said heterologous polypeptide has biosynthetic activity, and produces compound of interest or intermediate.
12. host cell according to claim 11, wherein said compound of interest or intermediate comprise VITAMIN, amino acid, microbiotic, carbohydrate or tensio-active agent.
13. host cell according to claim 12, wherein said carbohydrate comprises hyaluronic acid.
14. according to the arbitrary described host cell of claim 4-9, wherein said heterologous polypeptide is an enzyme, preferred secretor type enzyme.
15. host cell according to claim 14, wherein said enzyme are the enzymes that is selected from following enzyme: oxydo-reductase (EC1), transferring enzyme (EC2), lytic enzyme (EC3), lyase (EC4), isomerase (EC5), and ligase enzyme (EC6).
16. host cell according to claim 15, wherein said enzyme has the activity that is selected from following enzyme: aminopeptidase, amylase, amyloglucosidase, mannase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, at, Maltose 4-glucosyltransferase, deoxyribonuclease, esterase, tilactase, beta-galactosidase enzymes, glucoamylase, glucose oxidase, Polyglucosidase, haloperoxidase, hemicellulase, saccharase, isomerase, laccase, ligase enzyme, lipase, lyase, mannosidase, oxydase, polygalacturonase, peroxidase, phytase, phenol oxidase, polyphenoloxidase, proteolytic enzyme, rnase, transferring enzyme, trans-glutaminases and zytase.
17. host cell according to claim 16, wherein said enzyme are amylase or mannase.
18. in Bacillus licheniformis mutant host cell, produce the method for at least a interested product, it is included in the Bacillus licheniformis mutant host cell of cultivating the arbitrary qualification of claim 1-17 in the suitable medium, produces described product in this substratum.
19. method according to claim 18, it also comprises the described interested product of isolated or purified.
20. the purposes of the arbitrary Bacillus licheniformis mutant host cell that limits of claim 1-17 at least a interested product of preparation, it is included in cultivates described mutant host cell in the suitable substratum, produce described product in this substratum.
21. according to the purposes of claim 20, it also comprises the described interested product of isolated or purified.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200200531 | 2002-04-10 | ||
| DKPA200200531 | 2002-04-10 |
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| Publication Number | Publication Date |
|---|---|
| CN1659282A true CN1659282A (en) | 2005-08-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN038133164A Pending CN1659282A (en) | 2002-04-10 | 2003-03-25 | Bacillus licheniformis mutant host cell |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20050176097A1 (en) |
| EP (3) | EP1696035A3 (en) |
| CN (1) | CN1659282A (en) |
| AU (1) | AU2003214036A1 (en) |
| WO (1) | WO2003087149A2 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101298604B (en) * | 2008-06-25 | 2011-05-04 | 天津科技大学 | High-temperature acid-resistant alpha-amylase mutant strain and construction method thereof |
| CN103540562A (en) * | 2006-11-30 | 2014-01-29 | 诺维信公司 | Dnase expression recombinant host cells |
| CN108291212A (en) * | 2015-10-14 | 2018-07-17 | 诺维信公司 | Polypeptide variants |
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| EP3018140A3 (en) | 2004-01-09 | 2016-08-17 | Basf Se | Inactivation of glutamylpolypeptide synthesis in bacillus |
| JP4485341B2 (en) * | 2004-12-20 | 2010-06-23 | 花王株式会社 | Recombinant microorganism |
| ES2755775T3 (en) | 2005-11-28 | 2020-04-23 | Dsm Ip Assets Bv | Enzyme preparation that produces a pure flavor |
| CN105393099B (en) | 2013-06-11 | 2018-06-19 | 丹佛斯有限公司 | Thin film sensor |
| WO2016205397A2 (en) * | 2015-06-15 | 2016-12-22 | Klibanov Alexander L | Target-specific delivery of therapeutic agents |
| CN111269295B (en) * | 2020-03-09 | 2022-03-22 | 河北农业大学 | Novel antibacterial peptide and preparation method and application thereof |
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| WO2022269081A1 (en) | 2021-06-24 | 2022-12-29 | Basf Se | Bacillus licheniformis host cell for production of a compound of interest with increased purity |
| CN114807100B (en) * | 2022-04-28 | 2023-06-27 | 湖北大学 | Alkaline protease gene sequence suitable for bacillus licheniformis expression and application |
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| GB9708452D0 (en) * | 1997-04-26 | 1997-06-18 | Univ Newcastle | Improved prokaryotic expression of protein |
| CN1160448C (en) | 1998-02-12 | 2004-08-04 | 诺维信公司 | Prokaryotic cell comprising two copies of gene transcribed in different directions |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| EP1060253A2 (en) * | 1998-03-04 | 2000-12-20 | Genencor International, Inc. | Modified forms of pullulanase |
| AU2001262063A1 (en) | 2000-05-24 | 2001-12-03 | Novozymes A/S | Method for increasing gene copy number in a host cell and resulting host cell |
| WO2002000907A1 (en) * | 2000-06-23 | 2002-01-03 | Novozymes A/S | Method for stable chromosomal multi-copy integration of genes |
| EP1355931A2 (en) * | 2000-10-06 | 2003-10-29 | Novozymes Biotech, Inc. | Methods for monitoring multiple gene expression |
-
2003
- 2003-03-25 AU AU2003214036A patent/AU2003214036A1/en not_active Abandoned
- 2003-03-25 EP EP06115212A patent/EP1696035A3/en not_active Withdrawn
- 2003-03-25 CN CN038133164A patent/CN1659282A/en active Pending
- 2003-03-25 EP EP03709681A patent/EP1497431A2/en not_active Withdrawn
- 2003-03-25 EP EP09172174A patent/EP2213745A1/en not_active Withdrawn
- 2003-03-25 WO PCT/DK2003/000201 patent/WO2003087149A2/en not_active Application Discontinuation
- 2003-03-25 US US10/510,812 patent/US20050176097A1/en not_active Abandoned
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103540562A (en) * | 2006-11-30 | 2014-01-29 | 诺维信公司 | Dnase expression recombinant host cells |
| CN101298604B (en) * | 2008-06-25 | 2011-05-04 | 天津科技大学 | High-temperature acid-resistant alpha-amylase mutant strain and construction method thereof |
| CN108291212A (en) * | 2015-10-14 | 2018-07-17 | 诺维信公司 | Polypeptide variants |
| US12060589B2 (en) | 2015-10-14 | 2024-08-13 | Novozymes A/S | DNase variants |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003214036A8 (en) | 2003-10-27 |
| AU2003214036A1 (en) | 2003-10-27 |
| EP2213745A1 (en) | 2010-08-04 |
| WO2003087149A2 (en) | 2003-10-23 |
| EP1696035A2 (en) | 2006-08-30 |
| WO2003087149A3 (en) | 2004-03-18 |
| EP1497431A2 (en) | 2005-01-19 |
| EP1696035A3 (en) | 2008-04-02 |
| US20050176097A1 (en) | 2005-08-11 |
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