CN1304056C - Suspension-aid agent for vaccine of coccidiosis - Google Patents
Suspension-aid agent for vaccine of coccidiosis Download PDFInfo
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- CN1304056C CN1304056C CNB2005100360046A CN200510036004A CN1304056C CN 1304056 C CN1304056 C CN 1304056C CN B2005100360046 A CNB2005100360046 A CN B2005100360046A CN 200510036004 A CN200510036004 A CN 200510036004A CN 1304056 C CN1304056 C CN 1304056C
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Abstract
The present invention discloses a suspension agent of a coccidiosis vaccine, which is characterized in that the suspension agent is composed of (the weight of the suspension agent is represented by 100%) 5% to 20% of a carboxymethyl cellulose, 0.5% to 3% blowing powder and oligosaccharide as the rest The suspension agent has the advantages that coccidium ovicysts can be evenly suspended in drinking water and the coccidian ovicysts do not settle for more than 6 hours. On one hand, the coccidiosis vaccine does not need to cut off water supply before inoculation, and the present invention has the advantages of convenience, stress reduction and avoidance of generation of a water snatch phenomenon; on the other hand, the water drinking time is prolonged, the water intake of chickens is relatively even, and the uniformity of the inoculation of the coccidiosis vaccine is finally ensured. The present invention provides a convenient and feasible scheme for the popularization and the application of the coccidiosis vaccine.
Description
Technical field:
The present invention relates to the invention belongs to live vaccine immunity inoculation suspending agent, or rather, the present invention is that a kind of suspending effect that is combined by multiple composition reaches the coccidiosis vaccine suspending agent more than 6 hours, makes coccidiosis vaccine immunity inoculation approach adopt the drinking-water inocalation method that need not in advance cut off the water to become possibility.
Background technology:
Chicken coccidiosis is one of the most serious disease of harm intensification poultry husbandry, and its harm to chicken is suitable with some deadly infectious diseases.So far, the main means of the anti-system of chicken coccidiosis are to use coccidiostat to carry out chemical prevention.Yet along with extensively and continuously using of coccidiostat, drug resistance and drug residue two large problems are on the rise, and prevent the research of the coccidiosis of chicken to cause extensive attention by immunity.The factor that influences immune effect is various, and the immunization method of science is to guarantee to induce necessary guarantee strong, lasting, even immunity.The live only immunity inoculation approach of egg capsule vaccine of the weak poison of chicken coccidiosis is oral, by a drip nozzle to chicken stress be big, and waste time and energy during immunity; Coccidian oocyst very fast death and can not admix and allow the chicken long period search for food in the feedstuff in dry environment; The drinking-water immune operation is simple, stress be little to the chicken group, but because of egg capsule density is big, and sink to the water fountain bottom soon and cause most of vaccine can not bring into play due effect, swim in suspending agent in the drinking-water so must add a kind of egg capsule that can make when immune.According to relevant, though currently used suspending agent can make egg capsule be suspended in the drinking-water within a certain period of time, but it is very short to hold time, generally be no more than 2 hours,, must cut off the water before the immunity in advance for the diseases caused by retention of fluid that allows the chicken group will be mixed with vaccine in 2 hours finishes, this often causes the chicken group robbing drinking water, some strong chickens drink too much and the part weakling drinks very fewly, even do not drink, and cause immunizing dose inhomogeneous.When the vaccine egg capsule through disseminating a large amount of filial generation egg capsules after the chicken group breeding in feces and bedding and padding the time, leak among the chicken group and exempt from or the insufficient chicken of immunizing dose will be because of lacking the not enough severe infection that take place of fundamental immunity or fundamental immunity, thereby hemafecia appears and weightening finish is obstructed, even dead, have a strong impact on chicken group's fertility performance.
Summary of the invention:
Purpose of the present invention is exactly some shortcomings that overcome prior art, explore the persistent coccidiosis vaccine suspending agent of a kind of suspending effect, need not cut off the water and immunity inoculation dosage effect of uniform before reaching immunity, to solve the methods of vaccination problem of coccidiosis vaccine in advance.
The present invention realizes by following technical proposals: suspending agent of the present invention is by carboxymethyl cellulose, oligosaccharide and dries up powder and constituted, its constituent content (is 100% in suspending agent weight): carboxymethyl cellulose 5%～20%, dry up powder 0.5%～3%, all the other are oligosaccharide.
Carboxymethyl cellulose has another name called CMC (abbreviation of English Carloxy Methyl Cellulose), is a kind of cellulose ether of polymeric anion type.Outward appearance is white or micro-yellow powder, odorless, nontoxic, the easy moisture absorption, slightly saline taste arranged, its substitution value was an aqueous greater than 0.4 o'clock, along with substitution value rises, the corresponding improvement of water miscible transparency, it is insoluble to organic solvents such as acid, alcohol, ether, chloroform, benzene, but water-soluble and form the colloid solution of stable transparent.Carboxymethyl cellulose has multiple excellent properties such as thickening, suspension, dispersion, emulsifying, bonding, water conservation, protecting colloid, can be used as emulsifying agent, thickening agent, dispersant, stabilizing agent etc., and the laudatory title of " industrial monosodium glutamate " is arranged.
Oligosaccharide claims oligosaccharide again, be meant the general name of 2～10 monosaccharide with the chemical compound of glycosidic bond connection, mainly comprise: oligomeric lactose, oligomeric galactose, oligofructose, oligomeric isomaltose, palatinose, oligomeric dragon gallbladder sugar, soybean oligo saccharide, cottonseed sugar, stachyose etc. are that the health product as the people come into the market at first.It is reported, in feedstuff, add an amount of oligosaccharide, can improve the microecological environment in the poultry intestinal, improve the immunity of poultry, promote growth, have the effect that is similar to probiotics.Its immunoreation tends to the Intestinum Gallus domesticus road is caused slight damaging action after the coccidiosis vaccine immunity, influences the weightening finish of chicken body in a short time, adds a certain amount of oligosaccharide in the coccidiosis vaccine suspending agent, to overcoming this problem certain help will be arranged.On the other hand, oligosaccharide is a kind of glucide, can be used as dispersing aid, increase to disperse the viscosity of media, and the surface that is adsorbed on suspended particles increases its hydrophilic, helps reducing the sedimentation velocity of microgranule and coalescent, increases the stability of suspensoid.
Drying up powder is a kind of desiccant, and during preservation the moisture absorption is rotten to prevent carboxymethyl cellulose and oligosaccharide.
The present invention is by with above-mentioned three kinds of suspending agents that material combines by a certain percentage, can make coccidian oocyst be suspended in the drinking-water equably and can keep more than 6 hours and not sedimentation.On the one hand, need not cut off the water before the coccidiosis vaccine inoculation in advance, not only saved trouble but also reduce stress, avoid occurring the chicken group and rob the water phenomenon; On the other hand, prolong the drinking-water time, the chicken amount of drinking water is even relatively.This finally guarantees the uniformity of coccidiosis vaccine inoculation, for applying of coccidiosis vaccine provides simple and feasible scheme.
The specific embodiment
Suspending agent of the present invention dries up powder by 15% carboxymethyl cellulose, 83% oligosaccharide and 2% and constitutes (is 100% in suspending agent weight) through mixing.
Further specify effect of the present invention with example below:
The test of example 1 egg capsule sedimentation static measurement
1 materials and methods:
1.1 coccidian oocyst mixed liquor: contain Eimeria tenella (Eimeria tenella), poison Eimeria (E.necatrix), Eimeria maxima (E.maxima) and 4 kinds of heap type Eimeria (E.acervulina), with 1: 1: 1: 1 ratio mixing was made into the coccidiosis vaccine liquid of 1,000,000 egg capsule/ml with 2.5% potassium bichromate solution.
1.2 test grouping and processing:
One group: the coccidian oocyst mixed liquor is fully shaken up, draws 2ml and join in the 1000ml drinking-water, from different directions (clockwise, counterclockwise, up and down) stir repeatedly; Take by weighing suspending powder 7g of the present invention, slowly add in the above-mentioned coccidian oocyst liquid, the limit edged from different directions (clockwise, counterclockwise, up and down) stir repeatedly, dissolve fully until suspending agent, coccidian oocyst liquid in the pasty state.
Two groups: take by weighing the coccidiosis vaccine suspending agent 20g that certain company sells by operation instruction, slowly join in the 1000ml drinking-water, from different directions (clockwise, counterclockwise, up and down) stir repeatedly, dissolve fully until suspending agent; The coccidian oocyst mixed liquor is fully shaken up, draws 2ml and join in the above-mentioned solution, from different directions (clockwise, counterclockwise, up and down) stir repeatedly.
Three groups (blank group): the coccidian oocyst mixed liquor is fully shaken up, draws 2ml and join in the 1000ml drinking-water, from different directions (clockwise, counterclockwise, up and down) stir repeatedly.
Draw every group of supernatant liquid 15 μ l respectively at 0h, 1h, 2h, 4h, 6h, 10h with liquid-transfering gun, place blood counting chamber to carry out the egg capsule counting, count from 6 sample point imbibitions at every turn, get its meansigma methods for every group.
2 results
Suspending powder of the present invention can make coccidian oocyst be suspended in equably in the drinking-water and can keep the above and not sedimentation of 6h; And the coccidiosis vaccine suspending agent that certain company sells during to 2h egg capsule descended 67.1%.
Concrete data see Table 1:
Table 1 coccidiosis vaccine suspending agent laboratory static measurement result of the test
| Group | Time (h) | Measured data (individual egg capsule/15 μ l) | Average (individual egg capsule/15 μ l) | Ratio (%) |
| First group | 0 | 28、32、30、26、29、33 | 29.7 | 100 |
| 1 | 30、32、29、28、31、27 | 29.5 | 99.3 | |
| 2 | 28、27、30、31、28、32 | 29.3 | 98.7 | |
| 4 | 25、29、30、26、29、33 | 28.7 | 96.6 | |
| 6 | 29、23、29、26、31、32 | 28.3 | 95.3 | |
| 10 | 26、24、29、22、27、20 | 24.7 | 83.2 | |
| Second group | 0 | 27、29、32、31、29、29 | 29.5 | 100 |
| 1 | 13、17、11、19、16、21 | 16.2 | 54.9 | |
| 2 | 7、10、11、8、13、9 | 9.7 | 32.9 | |
| 4 | 2、5、1、4、4、6 | 3.7 | 12.5 | |
| 6 | 0、1、1、0、2、3 | 1.2 | 4.1 | |
| 10 | 0、0、0、0、0、0 | 0 | 0 | |
| The 3rd group | 0 | 30、28、32、26、32、33 | 30.2 | 100 |
| 1 | 3、1、3、2、2、4 | 2.5 | 8.3 | |
| 2 | 0、1、1、0、0、0 | 0.3 | 1.0 | |
| 4 | 0、0、0、0、0、0 | 0 | 0 | |
| 6 | 0、0、0、0、0、0 | 0 | 0 | |
| 10 | 0、0、0、0、0、0 | 0 | 0 |
Effect comparative test 1 materials and methods of the different methods of vaccination of example 2 chicken coccidiosis attenuated vaccines:
1.1 test chickling
3000 the 1 assorted commodity baby chicks of age in days stone fork available from hilllock, brilliant ruins chicken house, adopt the ground bedding and padding flat foster, free choice feeding.
The egg capsule tetravalent vaccine 1.2 the weak poison of chicken coccidiosis is lived (positive allusion quotation ball Seedling--COVAC)
Develop by our unit, contain Eimeria tenella (Eimeria tenella), poison Eimeria (E.necatrix), Eimeria maxima (E.maxima) and 4 kinds of heap type Eimeria (E.acervulina), immunizing dose is 1200 egg capsule/chickens.
1.3 feedstuff
Provide by the Guangdong Province Guanghong Jiujiang Feed Co., Ltd, be complete feedstuff, do not add any coccidiostat.
1.4 EXPERIMENTAL DESIGN
Above-mentioned 3000 test chickling are divided into 3 groups, 1000 every group at random.The 1st group is the drinking-water immune group of cutting off the water, and the 2nd group is the drinking-water immune group of not cutting off the water, and the 3rd group is the drip nozzle immune group, and every group is carried out immunity inoculation when 4 ages in days.Concrete processing is as follows:
The 1st group: the 2h that cuts off the water earlier before the immunity, the immunity inoculation of drinking water again coccidiosis vaccine.Be 5kg water earlier by the required water yield of this group of the about 5ml weighing of the water yield that needs to drink in behind every chicken controlling the water circulation 2 hours, add coccidiosis vaccine suspending agent 1 bag that certain company sells by operation instruction, from different directions (clockwise, counterclockwise, up and down) stir repeatedly, add the positive allusion quotation ball of 1000 plumage parts Seedling (fully shaking up) again with preceding, from different directions (clockwise, counterclockwise, up and down) stir repeatedly.Above-mentioned vaccine liquid is assigned in 10 water fountains equably, is placed into equably and allows chicken freely drink in the hen house, until the intact drink clear water that changes again of whole drinks.
The 2nd group: must not cut off the water the immunity inoculation of directly drinking water coccidiosis vaccine before the immunity.Earlier the required water yield of this group of the about 6ml weighing of the water yield that needs to drink in 4～6 hours at ordinary times by every chicken is a 6kg water, add the positive allusion quotation ball of 1000 plumage parts Seedling (fully shaking up) again with preceding, from different directions (clockwise, counterclockwise, up and down) stir repeatedly, slowly pour 50g suspending powder of the present invention then into, the limit bevelling from different directions (clockwise, counterclockwise, up and down) stir repeatedly, dissolve fully until the suspending powder, vaccine liquid in the pasty state.Above-mentioned vaccine liquid is assigned in 10 water fountains equably, is placed into equably and allows chicken freely drink in the hen house, until the intact drink clear water that changes again of whole drinks.
The 3rd group:, calculate every plumage by 1 bottle of 80ml (1000 plumages part/bottle) and drip 2 (0.04ml/ drips) by a positive allusion quotation ball Seedling of chicken drip nozzle immunity.
1.5 observation index
1.5.1 clinical observation
During the whole test, carry out itemized record every day, comprise chicken group's health status, the chicken number of morbidity, death, diagnostic result, medicine expends usefulness, materials amount etc.
1.5.2 stool examination
Gather each 20 parts in fresh excreta sample from a plurality of positions on every group of hen house ground during respectively at 10,20,30,40 ages in days, restrain feces coccidian oocyst counting (OPG), discharge situation with the monitoring egg capsule.
1.5.3 weight ratio
Each is organized and randomly draws 30～60 chickens by only weighing in every 2 weeks, adopts SPSS software that data are carried out statistical analysis.
1.5.4 the statistics of survival rate and feedstuff-meat ratio
After the off-test, statistics is respectively organized survival rate and the feedstuff-meat ratio of chicken.
2 results
Respectively organize clinical manifestation 2.1 test the full phase
Hemafecia in various degree all appears in immunity back the 13rd～14 day, 3 groups of chickens, and the most serious with the 1st group especially, every square metre of area has 10～15 heaps approximately, and extremely chicken is 9; And the 2nd group and the 3rd group of symptom are lighter, and every square metre of area only has 1～3 heap hemafecia.Use ball speed gram immediately and treat, situation turns for the better by and large in the time of the 15th day to the immunity back, and coccidiosis is controlled, finds no clinical coccidiosis later on again.
2.2 each is organized death, survives situation
Duration of test, each group all has chicken death, identifies that through cuing open inspection the 1st group is 0.9% because of the coccidiosis mortality rate; And the 2nd group and the 3rd group be non-coccidiosis and cause death.Concrete condition sees Table 2.
Table 2 is respectively organized the death condition statistics
| Group | Original number of elements | Non-coccidiosis death condition | Because of the coccidiosis death condition | Survival rate (%) | ||
| Death toll | Mortality rate (%) | Death toll | Mortality rate (%) | |||
| The 1st group | 1000 | 18 | 1.8 | 9 | 0.9 | 97.3 |
| The 2nd group | 1000 | 19 | 1.9 | 0 | 0 | 98.1 |
| The 3rd group | 1000 | 16 | 1.6 | 0 | 0 | 98.4 |
2.3 respectively organize coccidian oocyst discharge situation in the feces
Each is organized, and coccidian oocyst discharge situation sees Table 3 in the feces.
Table 3 is respectively organized coccidiosis recall rate and the gram feces egg capsule counting (OPG) of chicken
| Group | 10 ages in days | 20 ages in days | 30 ages in days | 40 ages in days | ||||
| Recall rate | OPG | Recall rate | OPG | Recall rate | OPG | Recall rate | OPG | |
| The 1st group | 100％ (20/20) | 14221±5993 | 100％ (20/20) | 5614±3652 | 55％ (11/20) | 240±87 | 100％ (20/20) | 8916±4557 |
| The 2nd group | 100％ (20/20) | 31745±1025 * | 95％ (19/20) | 1460±541 | 25％ (5/20) | 90±49 | 100％ (20/20) | 2783±1080 |
| The 3rd group | 100％ (20/20) | 7425±1167 | 100％ (20/20) | 3925±2988 | 30％ (6/20) | 235±194 | 95％ (19/20) | 7075±2022 |
Annotate: adopt the S-N-K method (being the q method of inspection) of SPSS software, * represents that the 2nd group is compared significant difference (P＜0.05) with the 1st group, the 3rd group.
As seen from Table 3,3 groups all when 10d egg sac number the highest, descend later on rapidly, 30d drops to minimum point, slightly gos up during to 40d.This variation tendency is the most obvious with the 2nd group (do not cut off the water drinking-water immune group) especially, and its OPG value of 10d reaches 31745 ± 1025, is significantly higher than the 1st group and the 3rd group (P＜0.05), reduces to 1460 ± 541 rapidly during 20d, and its OPG value is lower during to 30d, has only 90 ± 49.
2.4 respectively organize the average weight of each growth stage
As seen from Table 4, the average weight with the 1st group (the drinking-water immune group of cutting off the water) when 14d is lower, and then lower with the 3rd group of (drip nozzle immune group) counterpoise when 28d, 42d, it is not remarkable that all the other each ages in days are respectively organized counterpoise difference.
Table 4 is respectively organized the average weight of each growth stage
| Group | 14d | 28d | 42d | 52d |
| The 1st group | 198.90±3.44 a | 619.36±7.25 c | 1309.56±17.74 e | 1723.70±18.10 f |
| The 2nd group | 206.80±3.94 ab | 626.35±7.31 c | 1325.28±19.32 e | 1725.42±24.52 f |
| The 3rd group | 216.30±2.87 b | 604.02±6.49 c | 1244.30±16.63 d | 1681.38±20.04 f |
Annotate: adopt the S-N-K method (being the q method of inspection) of SPSS software, different days differences not significantly (P＞0.05) between the identical group of subscript or on the same group, between the different group of subscript or on the same group different days differences significantly (P＜0.05).
2.5 test the price of deed of full phase
As seen from Table 5, testing its feedstuff-meat ratio no significant difference of full phase for 3 groups.
The table 5 full phase of test is respectively organized feed conversion rate
| Group | Total augment weight (kg) | Total feed consumption (kg) | Feedstuff-meat ratio |
| The 1st group | 1721.2 | 3774.5 | 2.19 |
| The 2nd group | 1747.1 | 3780.0 | 2.16 |
| The 3rd group | 1691.6 | 3672.5 | 2.17 |
3 conclusions
From above-mentioned every index, the immune result of the inoculation method of the 2nd group of immunity of not cutting off the water is comparatively desirable, and the drinking-water immunity of taking not cut off the water, and chicken is avoided stress because of what cut off the water, catch chicken.
Claims (2)
1, a kind of coccidiosis vaccine suspending agent, it is characterized in that this suspending agent is by carboxymethyl cellulose, oligosaccharide and dries up powder and constituted that its constituent content is 100% in suspending agent weight: carboxymethyl cellulose 5%～20%, dry up powder 0.5%～3%, all the other are oligosaccharide.
2, coccidiosis vaccine suspending agent according to claim 1, it is characterized in that described oligosaccharide is one or more the combination in oligomeric lactose, oligomeric galactose, oligofructose, oligomeric isomaltose, palatinose, oligomeric dragon gallbladder sugar, soybean oligo saccharide, cottonseed sugar, the stachyose.
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| WO2023110658A1 (en) * | 2021-12-13 | 2023-06-22 | Dsm Ip Assets B.V. | Use of oligosaccharide preparation to increase performance of animal vaccinated against coccidiosis |
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| CN102727900B (en) * | 2012-07-05 | 2014-05-07 | 烟台青大生物制药有限公司 | Chicken coccidiosis vaccine suspending agent and fluid suspension therewith |
| CN102727902A (en) * | 2012-07-05 | 2012-10-17 | 烟台青大生物制药有限公司 | Use method for chicken coccidiosis vaccine suspending agent and preparation method for suspension |
| CN104873970A (en) * | 2015-05-27 | 2015-09-02 | 山西农业大学 | Coccidian vaccine immunopotentiation suspending agent and preparation method thereof |
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| CN108126208A (en) * | 2017-12-30 | 2018-06-08 | 天津赫莱恩特生物科技有限公司 | A kind of preparation method of aerosol type suspending agent and its application on coccidial vaccine |
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| CN110448528B (en) * | 2019-09-09 | 2024-06-04 | 佛山市正典生物技术有限公司 | Suspending agent for immunization, preparation of vaccine suspension and spray-drop immunization method |
| CN111330001B (en) * | 2020-03-24 | 2022-05-20 | 中国农业大学 | Suspending agent and suspension of rabbit coccidiosis attenuated live vaccine |
| CN114146169B (en) * | 2021-12-28 | 2023-08-25 | 佛山市正典生物技术有限公司 | Carrier composition for chicken coccidium spray-drop immunization, using method and chicken coccidium spray-drop immunization composition |
| CN116531497A (en) * | 2023-05-09 | 2023-08-04 | 佛山市正典生物技术有限公司 | Method and kit of suspension products for rapid hydration of short rheology suspension |
| CN116059331B (en) * | 2022-12-14 | 2023-09-19 | 佛山市正典生物技术有限公司 | Suspending agent granule and preparation method and application thereof |
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| US5814320A (en) * | 1989-03-28 | 1998-09-29 | Akzo Nobel N.V. | Eimeria tenella polypeptide and vaccine containing same |
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|---|---|---|---|---|
| US5814320A (en) * | 1989-03-28 | 1998-09-29 | Akzo Nobel N.V. | Eimeria tenella polypeptide and vaccine containing same |
| US5843722A (en) * | 1993-11-12 | 1998-12-01 | Akzo Nobel N.V. | Coccidiosis poultry vaccine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2023110658A1 (en) * | 2021-12-13 | 2023-06-22 | Dsm Ip Assets B.V. | Use of oligosaccharide preparation to increase performance of animal vaccinated against coccidiosis |
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