CN115791654A - Measuring device and measuring method - Google Patents

Abstract

The present invention relates to a measuring apparatus and a measuring method. The measuring device comprises a sensor, a signal output mechanism and a signal receiving mechanism, wherein the sensor comprises a plurality of detection areas which are arranged in an array; the signal output mechanism comprises an output optical fiber assembly and a light source corresponding to the input end of the output optical fiber assembly, the output optical fiber assembly comprises a plurality of output optical fibers which are arranged in an array, and the output optical fibers correspond to the detection areas one by one; the signal receiving mechanism is used for receiving the light reflected by the sensor. The invention improves the detection efficiency of the sample to be detected. A measuring method is also provided.

Description

Measuring device and measuring method

Technical Field

The invention relates to the technical field of optical sensing, in particular to a measuring device and a measuring method.

Background

The optical sensor has high performance in the aspects of drug detection, analysis of pesticide residues, pathogens, heavy metals and toxic substances, food and environmental safety monitoring and the like, and has the advantages of high sensitivity, high detection efficiency, no need of calibration and the like. Wherein the uncalibrated operation fully satisfies the established requirements for the characterization and identification of molecular constituents. In addition, by measuring the concentration of the analyte, the accuracy of the output, the concentration required to detect the sample, and the total time it takes to complete the analysis, a reusable and cleanable biosensor can be realized. The optical biosensor characterizes a sample to be measured by quantitatively measuring optical basic characteristics such as phase, amplitude, polarization and the like, and is widely researched based on the characteristics, including a local surface plasmon sensor, a surface enhanced raman scattering sensor, an optical fiber sensor, an evanescent wave fluorescence sensor and the like.

The biosensor of surface plasmon resonance is the main optical biosensing method at present, and the technology enables high sensitivity and real-time interactive measurement without any calibration. The surface plasmon resonance phenomenon occurs at an interface of a metal and a medium, and when the interface is irradiated with polarized light at a specific angle, surface plasmon is generated, which changes the intensity of reflected light, and a sensing pattern can be obtained by measuring a change in reflectance, angle, or wavelength. The surface plasmon resonance sensor can realize direct, label-free and real-time refractive index change on the surface, and the change rule is related to the concentration of biomolecules.

However, most of the currently widely studied surface plasmon sensors are one-dimensional detection methods, that is, when a biological sample to be detected is located on the surface of the sensor, a light source is irradiated onto the surface of the sensor, and the sample to be detected is analyzed by analyzing a spectral signal of the sample to be detected, but when the sample to be detected contains a plurality of components to be detected, each component in the sample to be detected cannot be accurately positioned by using the one-dimensional sensor, and the detection efficiency is low.

Disclosure of Invention

In view of the above, it is necessary to provide a measuring apparatus for improving the detection efficiency of a sample to be detected. A measuring method is also provided.

A measurement device, the measurement device comprising:

a sensor including a plurality of detection regions arranged in an array;

the signal output mechanism comprises an output optical fiber assembly and a light source corresponding to the input end of the output optical fiber assembly, the output optical fiber assembly comprises a plurality of output optical fibers which are arranged in an array, and the output optical fibers correspond to the detection areas one by one; and

a signal receiving mechanism for receiving light reflected via the sensor.

Most of the surface plasmon sensors widely researched at present are one-dimensional detection methods, namely, when a biological sample to be detected is positioned on the surface of the sensor, a light source is irradiated to the surface of the sensor, and the sample to be detected is analyzed by analyzing a spectral signal of the sample to be detected. However, when the sample to be detected contains a plurality of components to be detected, each component in the sample to be detected cannot be accurately positioned by using the one-dimensional sensor, and the detection efficiency is low.

Embodiments of the present disclosure provide a measurement device wherein the sensor is capable of varying the intensity of reflected light. The light source sets up the input that is used for providing light at output optical fiber group, and output optical fiber's quantity is a plurality of, and a plurality of output optical fiber permutation are arranged, and signal receiving mechanism is used for receiving the light via the sensor reflection, because a plurality of output optical fiber set up with a plurality of detection area one-to-ones, the spectral signal of each detection area way output is observed to the accessible, judges the composition parameter of the sample that awaits measuring in different zones, and detection efficiency promotes greatly. Furthermore, the output optical fiber can be recycled for multiple times, and the detection cost can be effectively reduced.

In one embodiment, the signal output mechanism further comprises an output lens assembly connected to the output end of the output optical fiber assembly, and the output lens assembly comprises output lenses in one-to-one correspondence with the output optical fibers, and the output lenses are used for collimating the light projected from the output optical fibers.

So set up, output lens sets up at output fiber's output, and output lens and output fiber one-to-one set up, but the collimation is from the light that output fiber throws out, corresponds with the detection area better.

In one embodiment, the output lens is a graded-index collimator lens, and the refractive index n (r) of the output lens at the radius r satisfies the following relation:

wherein n (r) is the refractive index of the output lens at a radius r, r is the distance from the center of the output lens, n ₀ G represents a refractive index gradient constant for the refractive index of the output lens at r = 0.

By means of the arrangement, the collimating lens is combined with the output optical fibers arranged in the multi-channel array to process the optical signals by utilizing the light focusing, collimating and beam shaping capabilities of the collimating lens. Meanwhile, as the sensor is in a dot matrix form with a plurality of detection areas, the spectral intensity of output optical fiber channels corresponding to different detection areas can be measured through the signal receiving mechanism, samples are characterized, two-dimensional sensing measurement is realized, and the measurement efficiency is high.

In one embodiment, the length z of the output lens _l The following relation is satisfied:

wherein n is a natural number, and g represents a refractive index gradient constant.

With this arrangement, since n is a natural number, when p = n +0.25, a point source at infinity can be collimated; when p = n, the imaging size is consistent with the original image; when p = n +0.5, an inverted image is formed.

Since a point source at infinity can be collimated only when p = n +0.25, the size of p can be determined by the length of the output lens and hence the working distance of the output lens. The conventional measuring device does not have a lens structure, so that the working distance from the output optical fiber to the detection area must be shortened as much as possible in order to avoid the light output by the output optical fiber from diverging, which may affect the biological sample.

In the embodiment, the collimating lens is combined with the output optical fibers arranged in the multi-channel array to process the optical signals by utilizing the light focusing, collimating and beam shaping capabilities of the collimating lens, so that the response distance of the optical signals is increased while the measurement requirements are met, and the influence on the biological samples can be reduced.

In one embodiment, the signal receiving mechanism comprises a receiving fiber assembly and a spectrometer corresponding to an output end of the receiving fiber assembly.

In one embodiment, the receiving optical fiber assembly includes receiving optical fibers corresponding to the output optical fibers one by one, and the signal receiving mechanism further includes a receiving lens assembly connected to an input end of the receiving optical fiber assembly, and an output end of the receiving optical fibers is disposed toward the spectrometer.

So set up, output fiber and receiving fiber one-to-one, the input and the receiving lens subassembly of receiving fiber are connected, can guarantee like this that receiving fiber subassembly and receiving lens subassembly can receive the light of the detection area reflection from the sensor completely, and receiving effect is good. The output end of the receiving optical fiber faces the spectrometer, so that the spectral signal of the light can be analyzed by the spectrometer, the component parameters of the sample to be measured in the detection area at different positions are judged, and the measurement effect is good.

In one embodiment, the sensor includes a first metal layer, a second metal layer, and a dielectric layer connecting the first metal layer and the second metal layer, and the detection region is disposed on a surface of the first metal layer.

With this arrangement, when the parallel optical signal is irradiated to the first metal layer-dielectric layer-second metal layer, the surface plasmon resonance mode is excited, and the optical response of the heterogeneous component of the biological sample is different from the spectral response of the normal component. In addition, the output optical fibers arranged in the multi-channel array can be used for carrying out pixel point detection, the positions of different components are positioned according to pixel point test results, the collimating lens optical fiber array is used for transmitting optical signals, the working distance between the output optical fibers and the sensor is increased, the alignment tolerance of the output optical fibers is enlarged, a sample to be detected is divided into a plurality of detection areas, and the component parameters of different areas in the sample to be detected can be accurately judged by observing the spectral signals output by each output optical fiber channel.

The invention also provides a measuring method, which comprises the following steps:

step S1: placing samples to be detected on a plurality of detection areas of a sensor;

step S2: projecting light rays to a plurality of detection areas corresponding to the plurality of output optical fibers through the plurality of output optical fibers;

and step S3: the light is reflected to the signal receiving mechanism, so that the spectral signal of the light is analyzed through the signal receiving mechanism.

In the above embodiment, the sensor is rasterized into a plurality of detection areas, the sample to be detected is placed on the plurality of detection areas of the sensor, because the number of the output optical fibers is multiple, and the output optical fibers are arranged in one-to-one correspondence with the detection areas, the plurality of output optical fibers project light to the plurality of detection areas corresponding to the plurality of output optical fibers, the intensity of the light is changed by the sensor and reflected to the signal receiving mechanism, and the spectral signal of the light is analyzed by the signal receiving mechanism.

In one embodiment, step S2 includes:

step S21: providing, by a light source, light projected to a plurality of the output fibers;

step S22: and the light rays output from the output optical fibers are collimated and projected to the detection area through a plurality of output lenses.

So set up, provide the light that throws to a plurality of these output fiber through the light source, because output lens's quantity is a plurality ofly, and output lens and output fiber one-to-one, will throw to this detection area from the light collimation of a plurality of these output fiber outputs, utilize output lens's light focus and collimation and the ability of beam shaping, the output fiber combination that arranges collimating lens and multichannel array handles optical signal, when satisfying the measurement requirement, optical signal's response distance has been increased, can reduce the influence to the biological sample like this.

In one embodiment, in step S3, the spectrometer analyzes the spectrum signal of the light to determine the composition parameters of the detection area of the sample to be detected at different positions.

So set up, can judge the composition parameter of the sample that awaits measuring in this detection area of different positions more rapidly, detection efficiency is high.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments or the conventional technologies of the present application, the drawings used in the description of the embodiments or the conventional technologies will be briefly introduced below, it is obvious that the drawings in the description below are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic measurement diagram of a measurement apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a measurement apparatus according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a connection structure between an output optical fiber and an output lens according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a detection area according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of an output fiber and sensor configuration according to one embodiment of the present invention;

FIG. 6 is a flowchart of a measurement method according to an embodiment of the invention.

The reference numbers illustrate:

1. a sensor; 11. a first metal layer; 12. a second metal layer; 13. a dielectric layer; 14. detecting a region; 2. a signal output mechanism; 21. an output fiber optic assembly; 211. an output optical fiber; 22. a light source; 23. an output lens assembly; 231. an output lens; 3. a signal receiving mechanism; 31. receiving a fiber optic assembly; 32. a receiving lens assembly; 33. a spectrometer; 4. a first normal molecule; 5. a second normal molecule; 6. an abnormal molecule.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will recognize without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless explicitly specifically defined otherwise.

In the present invention, unless otherwise explicitly stated or limited, the terms "mounted," "connected," "fixed," and the like are to be construed broadly, e.g., as being permanently connected, detachably connected, or integral; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. As used herein, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are for purposes of illustration only and do not denote a single embodiment.

Most of the widely studied surface plasmon sensors at present are one-dimensional detection methods, that is, when a biological sample to be detected is located on the surface of the sensor, a light source is irradiated on the surface of the sensor, and the sample to be detected is analyzed by analyzing a spectral signal of the sample to be detected. However, when the sample to be detected contains a plurality of components to be detected, each component in the sample to be detected cannot be accurately positioned by using the one-dimensional sensor, and the detection efficiency is low.

Referring to fig. 1 to 6, a measuring apparatus and a measuring method according to an embodiment of the present invention improve the efficiency of detecting a sample to be detected.

With reference to fig. 1 to 5, the present invention provides a measuring device, which includes a sensor 1, a signal output mechanism 2 and a signal receiving mechanism 3, wherein the sensor 1 includes a plurality of detection regions 14 arranged in an array; the signal output mechanism 2 comprises an output optical fiber assembly 21 and a light source 22 corresponding to the input end of the output optical fiber assembly 21, the output optical fiber assembly 21 comprises a plurality of output optical fibers 211 arranged in an array, and the output optical fibers 211 correspond to the detection areas 14 one by one; the signal receiving means 3 is used to receive the light reflected by the sensor 1.

In the above embodiment, the sensor 1 is capable of changing the intensity of reflected light. Light source 22 sets up and is used for providing light at the input of output fiber 211 group, the quantity of output fiber 211 is a plurality of, and a plurality of output fiber 211 permutation are arranged, signal receiving mechanism 3 is used for receiving the light via sensor 1 reflection, because a plurality of output fiber 211 and a plurality of detection area 14 one-to-one set up, the accessible is observed the spectral signal of 14 output of each detection area, judge the composition parameter of the sample that awaits measuring in different regions, detection efficiency promotes greatly.

Furthermore, the output optical fiber 211 can be recycled for multiple times, and the detection cost can be effectively reduced.

In this embodiment, the sensor 1 divides the detection area 14 into a plurality of detection areas 14 arranged in a rectangular shape by rasterization, each detection area 14 corresponds to each output optical fiber 211 channel, the detection coverage rate is large, and the detection efficiency is high. In some other embodiments, each detection region 14 may have other shapes, such as circular, trapezoidal, etc., so long as the detection region 14 can correspond to the output fiber 211 channel.

Specifically, in the present embodiment, the bottom of the output fiber 211 is flush with the sensor 1, so that efficient coupling is achieved and the stability of the output fiber 211 can be adjusted.

In this embodiment, the signal output mechanism 2 further includes an output lens assembly 23 connected to the output end of the output optical fiber assembly 21, the output lens assembly 23 includes output lenses 231 corresponding to the output optical fibers 211 one by one, and the output lenses 231 are used for collimating the light projected from the output optical fibers 211.

With such an arrangement, the output lens 231 is disposed at the output end of the output optical fiber 211, and the output lens 231 and the output optical fiber 211 are disposed in a one-to-one correspondence manner, so that light projected from the output optical fiber 211 can be collimated, and can better correspond to the detection area 14.

Preferably, the output lens 231 is a graded-index collimator lens, and the refractive index n (r) of the output lens 231 at the radius r satisfies the following relation:

where n (r) is the refractive index of the output lens 231 at the radius r, r is the distance from the center of the output lens 231, n0 is the refractive index of the output lens 231 at r =0, and g represents the index gradient constant.

By means of the arrangement, the light focusing and collimating capability and the light beam shaping capability of the collimating lens are utilized, and the collimating lens is combined with the output optical fibers 211 arranged in the multi-channel array to process the optical signals. Meanwhile, as the sensor 1 is in a dot matrix with a plurality of detection areas 14, the signal receiving mechanism 3 can be used for measuring the spectral intensity of the output optical fiber 211 channels corresponding to different detection areas 14, so as to characterize the sample, thereby realizing two-dimensional sensing measurement and having high measurement efficiency. Because the collimating lens is at different radiuses r, the refractive index is different, so that the collimating lens can realize the purpose of collimating light rays.

Further, the length z of the output lens 231 ₁ The following relation is satisfied:

wherein n is a natural number, and g represents a refractive index gradient constant.

With this arrangement, since n is a natural number, when p = n +0.25, a point source at infinity can be collimated; when p = n, the imaging size is consistent with the original image; when p = n +0.5, an inverted image is formed.

Since a point source at infinity can be collimated only when p = n +0.25, the size of p can determine the length of the output lens 231 and thus the working distance of the output lens 231.

Since the conventional measuring apparatus does not have a lens structure, the working distance from the output fiber 211 to the detection area 14 must be shortened as much as possible in order to avoid divergence of the light output from the output fiber 211, which may affect the biological sample.

In the embodiment, the light focusing, collimating and beam shaping capabilities of the collimating lens are utilized, the collimating lens is combined with the output optical fibers 211 arranged in the multi-channel array to process the optical signals, the measurement requirements are met, meanwhile, the response distance of the optical signals is increased, the influence on a biological sample can be reduced, and the detection accuracy is high.

It will be appreciated that in some embodiments the signal receiving structure comprises a spectrometer 33, and that the spectrometer 33 may be adapted to analyze the spectral signal of the light reflected from the sensor 1, and to obtain different composition parameters in different detection regions 14.

In this embodiment, the signal receiving mechanism 3 includes a receiving optical fiber assembly 31 and a spectrometer 33 corresponding to an output end of the receiving optical fiber assembly 31, the receiving optical fiber assembly 31 includes receiving optical fibers (not shown) corresponding to the output optical fibers 211 one to one, the signal receiving mechanism 3 further includes a receiving lens assembly 32 connected to an input end of the receiving optical fiber assembly 31, and an output end of the receiving optical fiber is disposed toward the spectrometer 33.

So set up, output fiber 211 and receiving fiber one-to-one, the input end of receiving the optic fibre is connected with receiving lens subassembly 32, can guarantee like this that receiving fiber subassembly 31 and receiving lens subassembly 32 can be complete receive from the light of sensor 1's detection area 14 reflection, and it is effectual to receive.

The output end of the receiving optical fiber is arranged towards the spectrometer 33, so that the spectral signal of the light can be analyzed by the spectrometer 33, the composition parameters of the sample to be measured in the detection area 14 at different positions can be judged, and the measurement effect is good.

Referring specifically to fig. 5, in some embodiments, the end face of the output fiber 211 has a polished angle (shown as α in fig. 5), and the sensor 1 has a coupling angle (shown as θ in fig. 5) corresponding to the polished angle. By such arrangement, the polishing angle of the end face of the output optical fiber 211 is consistent with the coupling angle of the sensor 1, so that the transmission of optical signals can be ensured, and the accuracy of measurement can be further ensured.

Specifically, the sensor 1 in this embodiment is a surface plasmon resonance sensor 1, the sensor 1 includes a first metal layer 11, a second metal layer 12, and a dielectric layer 13 connecting the first metal layer 11 and the second metal layer 12, and the detection region 14 is disposed on the surface of the first metal layer 11. Specifically, the dielectric layer 13 is silicon dioxide having a refractive index of 1.45.

It should be noted that when parallel optical signals are irradiated to first metal layer 11, dielectric layer 13, and second metal layer 12, the surface plasmon resonance mode is excited, and the optical response of the heterogeneous component of the biological sample is different from the spectral response of the normal component.

In addition, the output optical fiber 211 that the multichannel array was arranged can carry out the pixel point and detect, according to the position of the different appearance composition of pixel point test result location, utilize collimating lens fiber array transmission light signal, increased the working distance of output optical fiber 211 with sensor 1 on the one hand, on the other hand has enlarged the alignment tolerance of output optical fiber 211, divide the sample that awaits measuring into a plurality of detection areas 14, through observing the spectral signal of each output optical fiber 211 passageway output, can accurately judge the composition parameter of different regions in the sample that awaits measuring.

In this embodiment, the spectral signal output by each output optical fiber 211 channel is observed through the signal receiving mechanism 3, so that the component parameters of different areas in the sample to be measured can be accurately determined.

Specifically, the surface of the sensor 1 covers a biological sample to be detected, the biological sample contains a heterogeneous component, as shown in fig. 4, the first normal molecule 4 and the second normal molecule 5 are normal molecules in the sample to be detected, and when the abnormal molecule 6 is found, the position of the abnormal molecule 6 in the detection area 14 can be accurately determined, so that the detection efficiency is high.

Referring to fig. 1 to 6, the present invention further provides a measurement method, including:

step S1: placing samples to be tested on a plurality of detection areas 14 of the sensor 1;

step S2: projecting light through the plurality of output optical fibers 211 to a plurality of detection areas 14 corresponding to the plurality of output optical fibers 211;

and step S3: the light is reflected to the signal receiving mechanism 3, so that the spectral signal of the light is analyzed by the signal receiving mechanism 3.

In the above embodiment, the sensor 1 is rasterized into the plurality of detection regions 14, the sample to be detected is placed on the plurality of detection regions 14 of the sensor 1, since the number of the output optical fibers 211 is plural, and the output optical fibers 211 and the detection regions 14 are arranged in a one-to-one correspondence, the plurality of output optical fibers 211 project the light to the plurality of detection regions 14 corresponding to the plurality of output optical fibers 211, the intensity of the light is changed by the sensor 1 and is reflected to the signal receiving mechanism 3, and the spectral signal of the light is analyzed by the signal receiving mechanism 3.

Specifically, step S2 includes:

step S21: providing, by a light source 22, light projected to a plurality of the output fibers 211;

step S22: the light output from the output optical fibers 211 is collimated and projected to the detection region 14 by the output lenses 231.

With such an arrangement, the light source 22 provides light projected to the plurality of output optical fibers 211, and since the number of the output lenses 231 is plural, and the output lenses 231 correspond to the output optical fibers 211 one by one, the light output from the plurality of output optical fibers 211 is collimated and projected to the detection area 14, and the collimating lens and the output optical fibers 211 arranged in the multi-channel array are combined to process the optical signal by using the light focusing, collimating and beam shaping capabilities of the output lenses 231, the measurement requirement is met, and the response distance of the optical signal is increased, so that the influence on the biological sample can be reduced.

In some embodiments, in step S3, the spectral signals of the light are analyzed by the spectrometer 33 to determine the composition parameters of the detection area 14 of the sample to be detected at different positions.

By the arrangement, the component parameters of the detection area 14 of the sample to be detected at different positions can be judged more quickly, and the detection efficiency is high.

All possible combinations of the technical features of the above embodiments may not be described for the sake of brevity, but should be considered as within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A measuring device, characterized in that the measuring device comprises:

a sensor including a plurality of detection regions arranged in an array;

the signal output mechanism comprises an output optical fiber assembly and a light source corresponding to the input end of the output optical fiber assembly, the output optical fiber assembly comprises a plurality of output optical fibers which are arranged in an array, and the output optical fibers correspond to the detection areas one by one; and

a signal receiving mechanism for receiving light reflected via the sensor.

2. The measurement device of claim 1, wherein the signal output mechanism further comprises an output lens assembly coupled to an output end of the output fiber assembly, the output lens assembly comprising output lenses in one-to-one correspondence with the output fibers, the output lenses for collimating light projected from the output fibers.

3. The measurement device of claim 2, wherein the output lens is a graded index collimator lens, and the refractive index n (r) of the output lens at the radius r satisfies the following relation:

wherein n (r) is the refractive index of the output lens at the radius r, and r is the distance from the center of the output lensA distance of n ₀ G represents a refractive index gradient constant for the refractive index of the output lens at r = 0.

4. A measuring device according to claim 2, characterized in that the length z of the output lens _l The following relation is satisfied:

wherein n is a natural number, and g represents a refractive index gradient constant.

5. The measurement device of claim 1, wherein the signal receiving mechanism comprises a receiving fiber assembly and a spectrometer corresponding to an output end of the receiving fiber assembly.

6. The measurement device of claim 5, wherein the receiving fiber assembly comprises receiving fibers in one-to-one correspondence with the output fibers, and the signal receiving mechanism further comprises a receiving lens assembly connected to an input end of the receiving fiber assembly, an output end of the receiving fibers being disposed toward the spectrometer.

7. The measuring device according to any one of claims 1 to 6, wherein the sensor comprises a first metal layer, a second metal layer, and a dielectric layer connecting the first metal layer and the second metal layer, and the detection region is provided on a surface of the first metal layer.

8. A method of measurement, comprising:

step S1: placing samples to be tested on a plurality of detection areas of a sensor;

step S2: projecting light rays to a plurality of detection areas corresponding to a plurality of output optical fibers through the plurality of output optical fibers;

and step S3: the light is reflected to the signal receiving mechanism, so that the spectral signal of the light is analyzed through the signal receiving mechanism.

9. The measurement method according to claim 8, wherein step S2 comprises:

step S21: providing, by a light source, light projected to a plurality of the output fibers;

step S22: and the light rays output from the output optical fibers are collimated and projected to the detection area through a plurality of output lenses.

10. The method according to claim 8, wherein in step S3, the spectral signal of the light is analyzed by a spectrometer to determine the composition parameters of the detection area of the sample to be detected at different positions.

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