CN115615964A - Detection method of multichannel surface plasma resonance biosensor - Google Patents
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Abstract
The invention relates to the technical field of detection of a plasma resonance biosensor, and discloses a detection method of a multichannel surface plasma resonance biosensor, which comprises the following steps: s1: simulating an optical fiber surface plasma resonance sensing model, and determining parameters of a sensor; s2: designing system hardware; s201: and (5) designing an optical path system. By using the optical fiber as a carrier for exciting the surface plasmon resonance effect, manufacturing a plurality of sensing areas on the surface of the optical fiber, laying different types of metal films and two-dimensional nano material films in different sensing areas, and realizing the surface plasmon resonance effect under different wavelengths by optimizing the types and parameters of film materials, so that accurate, rapid, high-sensitivity and multi-channel detection of hemoglobin concentration is realized, the detection efficiency is effectively improved, and the detection cost is reduced.
Description
Technical Field
The invention relates to the technical field of detection of a plasma resonance biosensor, in particular to a detection method of a multichannel surface plasma resonance biosensor.
Background
Hemoglobin (Hb) is an important globular hemoprotein in a human body, is responsible for transporting oxygen and carbon dioxide in human blood, plays a role in regulating the internal mechanism activity of the human body, and is an important physiological index for reflecting whether the human body is anaemia and other blood diseases, so that the hemoglobin is an important human health evaluation standard. Generally, the normal value of the human hemoglobin concentration is 130-170g/L for men; 115-150g/L female; the newborn is 170-200g/L. When the concentration of hemoglobin is lower than the normal range, anemia can be judged, and when the concentration of hemoglobin is higher than the normal range, abnormality of internal organs of a human body can be judged. The detection of the hemoglobin concentration is not only beneficial to identifying and diagnosing diseases, but also can provide a main basis for guiding blood transfusion and the like during operation, and plays a vital role in clinic. At present, invasive hemoglobin concentration detection is a conventional method used in clinical monitoring, the method sends collected blood samples of a subject into a blood analyzer for chemical detection, in the technical field of hemoglobin concentration detection, the result of the blood analyzer can be used as a true value, but various chemical reagents are needed to preprocess the blood samples in the detection process, the measurement process is complicated, and the test consumes a long time.
With the development of related subjects such as novel sensor technology, biomedical engineering, intelligent materials and the like, the surface plasmon resonance sensing technology provides a new method for detecting the concentration of hemoglobin. Surface Plasmon Resonance (SPR) is a physical optical phenomenon in which evanescent waves generated when light is incident on a metal-dielectric interface and Plasmon waves on a metal Surface resonate with each other when a specific condition is satisfied, so that reflected light energy is sharply reduced. The surface plasma resonance parameters are very sensitive to the concentration change of the detected hemoglobin attached to the metal surface, and the detection method also has the advantages of no need of marking a detection sample, real-time rapid detection, high sensitivity and the like, so that the hemoglobin concentration in blood can be accurately and rapidly detected, and the oxygenation state of organism tissue cells can be accurately reflected.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a detection method of a multichannel surface plasmon resonance biosensor.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a detection method of a multi-channel surface plasmon resonance biosensor comprises the following steps:
s1: simulating an optical fiber surface plasma resonance sensing model, and determining parameters of a sensor;
s2: system hardware design
S201: optical path system design
The optical path system comprises coupled optical fiber, grating, light source and photoelectric detector, and the selected grating and the photoelectric detector are combined in the spectrometer to form a complete optical path system
S202: light source selection
Selecting a broadband light source, and continuously and stably outputting high-power light within the wave band range of visible light;
s203: sensing fiber selection
After incident light is processed, one Y-shaped optical fiber end is divided into six beams which are coupled to enter a sensing optical fiber, after the incident light reaches a sensing probe, the light enters a metal film from a port 3 and interacts with a measured medium to generate a surface plasma resonance effect, light carrying surface plasma resonance information forms reflected light after the action of a holophote on the end surface of an optical fiber core, the reflected light enters the optical fiber through the port 3 and then is emitted out through a port 2, the cross section of the Y-shaped optical fiber end is formed by surrounding an optical fiber by six optical fiber beams, the six optical fiber beams are incident light, the incident light flux is increased, the sensitivity of a sensor is enhanced, and a central optical fiber is emergent light envelope and is finally coupled to enter an optical detection system;
s204: selecting gratings
Selecting a grating with the model of slit-3 for detection;
s205: selecting a photodetector
In a charge coupled detector device, where charges generated by photons are collected and stored in metal-oxide-semiconductor (MOS) capacitors so that pixel addressing can be accurately performed with minimal lag, the device has a two-dimensional detector with random or quasi-random pixel addressing, a CCD can be regarded as a plurality of photodetection analog shift registers, after the charges generated by photons are stored, they are recorded to a preamplifier line by line through a high-speed shift register in a nearly horizontal direction, the resulting signals are stored in a computer, the whole operation process of the CCD device is a charge coupled process, and thus the device is called a charge coupled device, and a linear CCD detector with CCD parameters of 3648 pixels is selected;
s206: spectrum instrument selection
S207: system resolution
Determining the spectral coverage range of the grating to be 605nm, and the spectral coverage ranges of the detector original number sub-gratings to be: dispersion (nm/pixel) = grating spectral range/number of detector elements
Then determining pixel resolution, selecting 25 μm notch with pixel resolution of 7.4 pixels according to pixel resolution of different size notch
And then calculating the optical resolution: optical resolution = chromatic dispersion pixel resolution
S3: pretreating the optical fiber, and polishing and grinding the cladding of the optical fiber;
s4: then, a gold film is laid on the outer wall of the optical fiber;
s5: then laying a black phosphorus film on the outer wall of the gold film;
s6: laying a barium titanate film on the outer wall of the black phosphorus film;
s7: laying a graphene film on the outer wall of the barium titanate film;
s8: then, the optical fiber is irradiated with light and detected by a photodetector, thereby determining a detection value.
Preferably, the sensing fiber is a multimode silica fiber with a core diameter of 105 μm.
Preferably, the light source is a halogen tungsten lamp selected from incandescent light sources, and the spectrum range of the light source is 360-2500nm, and the color temperature of the light source is 3100K.
Preferably, the linear etching density of the grating is 600 lines/nm, the spectral range is 650nm, the blaze wavelength is 500nm, and the light transmission efficiency wavelength band is 350-1000nm.
Preferably, the thickness of the gold thin film is 40nm.
Preferably, the thickness of the black phosphorus film, the barium titanate film and the graphene film is 20nm.
(III) advantageous effects
Compared with the prior art, the invention provides a detection method of a multichannel surface plasmon resonance biosensor, which has the following beneficial effects:
according to the detection method of the multichannel surface plasma resonance biosensor, optical fibers are used as carriers for exciting the surface plasma resonance effect, a plurality of sensing areas are manufactured on the surfaces of the optical fibers, different types of metal films and two-dimensional nanometer material films are laid in the different sensing areas, the surface plasma resonance effect under different wavelengths is realized by optimizing the types and parameters of the film materials, accurate, quick, high-sensitivity and multichannel detection of hemoglobin concentration is further realized, the detection efficiency is effectively improved, and the detection cost is reduced.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a flow chart of the present invention;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and intended to explain the present invention and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
In the present invention, unless otherwise explicitly stated or limited, the terms "mounted," "connected," "fixed," and the like are to be construed broadly, e.g., as being permanently connected, detachably connected, or integral; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through the use of two elements or the interaction of two elements. The specific meanings of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.
As shown in FIG. 1, the present invention provides a method for detecting a multi-channel surface plasmon resonance biosensor, comprising the following steps:
s1: simulating an optical fiber surface plasma resonance sensing model, and determining parameters of a sensor;
s2: system hardware design
S201: optical path system design
The optical path system comprises coupled optical fiber, grating, light source, and photodetector, and the selected grating and photodetector are combined in the spectrometer to form complete optical path system
S202: light source selection
Selecting a broadband light source, and continuously and stably outputting high-power light within the wave band range of visible light; the light source is a tungsten halogen lamp of an incandescent light source, the spectral range of the tungsten halogen lamp is 360-2500nm, the color temperature is 3100K, the output spectral line of the tungsten halogen lamp is very smooth, no fracture, sword peak or pit exists, the output is very stable, and the tungsten halogen lamp can continuously and stably output light with optimized high brightness and power.
S203: sensing fiber selection
The optical fiber is a sensing part of the whole system, is only used as a transmission medium of light waves and is used for transmitting incident and reflected optical signals; by adopting the multimode silica fiber with the core diameter of 105 mu m, enough spectral modes and optical power are ensured, thereby obtaining good test effect
After incident light is processed, the Y-shaped optical fiber end is divided into six beams which are coupled and enter a sensing optical fiber, after the incident light reaches a sensing probe, the incident light enters a metal film from a port 3 and interacts with a measured medium to generate a surface plasma resonance effect, light carrying surface plasma resonance information forms reflected light after the action of a holophote on the end surface of an optical fiber core, the reflected light enters the optical fiber through the port 3 and then is emitted out through a port 2, the section of the Y-shaped optical fiber end is formed by surrounding an optical fiber by six optical fiber beams, wherein the six optical fiber beams are incident light, the incident light flux is increased, the sensitivity of the sensor is enhanced, the central optical fiber is emergent light envelope, and the emergent light envelope is finally coupled and enters an optical detection system;
s204: selecting gratings
Selecting a grating with the model of slit-3 for detection; the linear etching density of the grating is 600 lines/nm, the spectral range is 650nm, the blaze wavelength is 500nm, and the light transmission efficiency wavelength band is 350-1000nm.
S205: selecting a photodetector
In a charge coupled detector device, charges generated by photons are collected and stored in metal-oxide-semiconductor (MOS) capacitors so that pixel addressing can be accurately performed with minimal lag, the device has a two-dimensional detector with random or quasi-random pixel addressing function, a CCD can be regarded as a plurality of photoelectric detection analog shift registers, after the charges generated by photons are stored, they are recorded to a preamplifier line by line through a high-speed shift register in a nearly horizontal direction, the resulting signals are stored in a computer, the whole working process of the CCD device is a charge coupled process, therefore, the device is called a charge coupled device, and a linear CCD detector with CCD parameters of 3648 pixels is selected;
s206: spectrum instrument selection
S207: system resolution
Determining the spectral coverage range of the grating to be 605nm, and the spectral coverage range of the detector element number sub-grating to be: dispersion (nm/pixel) = grating spectral range/number of detector elements
Then determining pixel resolution, selecting 25 μm notch with pixel resolution of 7.4 pixels according to pixel resolution of different size notch
And then calculating the optical resolution: optical resolution = chromatic dispersion × pixel resolution
S3: pretreating the optical fiber, and polishing and grinding the cladding of the optical fiber;
s4: then, a gold film is laid on the outer wall of the optical fiber; the thickness of the gold thin film is 40nm.
S5: then laying a black phosphorus film on the outer wall of the gold film;
s6: laying a barium titanate film on the outer wall of the black phosphorus film;
s7: laying a graphene film on the outer wall of the barium titanate film; the thickness of the black phosphorus film, the thickness of the barium titanate film and the thickness of the graphene film are 20nm.
S8: then, the optical fiber is irradiated with light and detected by a photodetector, thereby determining a detection value.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising a reference structure" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Claims (6)
1. A detection method of a multi-channel surface plasma resonance biosensor is characterized by comprising the following steps: the method comprises the following steps:
s1: simulating an optical fiber surface plasma resonance sensing model, and determining parameters of a sensor;
s2: system hardware design
S201: optical path system design
The optical path system comprises coupled optical fiber, grating, light source, and photodetector, and the selected grating and photodetector are combined in the spectrometer to form complete optical path system
S202: light source selection
Selecting a broadband light source, and continuously and stably outputting high-power light within the wave band range of visible light;
s203: sensing fiber selection
After incident light is processed, the Y-shaped optical fiber end is divided into six beams which are coupled and enter a sensing optical fiber, after the incident light reaches a sensing probe, the incident light enters a metal film from a port 3 and interacts with a measured medium to generate a surface plasma resonance effect, light carrying surface plasma resonance information forms reflected light after the action of a holophote on the end surface of an optical fiber core, the reflected light enters the optical fiber through the port 3 and then is emitted out through a port 2, the section of the Y-shaped optical fiber end is formed by surrounding an optical fiber by six optical fiber beams, wherein the six optical fiber beams are incident light, the incident light flux is increased, the sensitivity of the sensor is enhanced, the central optical fiber is emergent light envelope, and the emergent light envelope is finally coupled and enters an optical detection system;
s204: selecting gratings
Selecting a grating with the model of slit-3 for detection;
s205: selecting a photodetector
In a charge coupled detector device, charges generated by photons are collected and stored in metal-oxide-semiconductor (MOS) capacitors so that pixel addressing can be accurately performed with minimal lag, the device has a two-dimensional detector with random or quasi-random pixel addressing function, a CCD can be regarded as a plurality of photoelectric detection analog shift registers, after the charges generated by photons are stored, they are recorded to a preamplifier line by line through a high-speed shift register in a nearly horizontal direction, the resulting signals are stored in a computer, the whole working process of the CCD device is a charge coupled process, therefore, the device is called a charge coupled device, and a linear CCD detector with CCD parameters of 3648 pixels is selected;
s206: spectrum instrument selection
S207: system resolution
Determining the spectral coverage range of the grating to be 605nm, and the spectral coverage range of the detector element number sub-grating to be: dispersion (nm/pixel) = grating spectral range/number of detector elements
Then determining pixel resolution, selecting 25 μm notch with pixel resolution of 7.4 pixels according to pixel resolution of different size notch
And then calculating the optical resolution: optical resolution = chromatic dispersion pixel resolution
S3: pretreating the optical fiber, and polishing and grinding the cladding of the optical fiber;
s4: then, a gold film is laid on the outer wall of the optical fiber;
s5: then laying a black phosphorus film on the outer wall of the gold film;
s6: laying a barium titanate film on the outer wall of the black phosphorus film;
s7: laying a graphene film on the outer wall of the barium titanate film;
s8: then, the optical fiber is illuminated and detected by a photodetector, thereby determining a detection value.
2. The method for detecting the multi-channel surface plasmon resonance biosensor according to claim 1, wherein: the sensing optical fiber adopts multimode silica optical fiber with the core diameter of 105 mu m.
3. The method for detecting the multi-channel surface plasmon resonance biosensor according to claim 1, wherein: the light source is a halogen tungsten lamp of an incandescent light source, and the spectral range of the halogen tungsten lamp is 360-2500nm, and the color temperature of the halogen tungsten lamp is 3100K.
4. The method for detecting the multi-channel surface plasmon resonance biosensor according to claim 1, wherein: the linear etching density of the grating is 600 lines/nm, the spectral range is 650nm, the blaze wavelength is 500nm, and the light transmission efficiency wavelength band is 350-1000nm.
5. The method for detecting the multi-channel surface plasmon resonance biosensor according to claim 1, wherein: the thickness of the gold thin film is 40nm.
6. The method for detecting the multi-channel surface plasmon resonance biosensor according to claim 1, wherein: the thickness of the black phosphorus film, the thickness of the barium titanate film and the thickness of the graphene film are 20nm.
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