CN115337219A - Skin repair composition and preparation method thereof - Google Patents
Skin repair composition and preparation method thereof Download PDFInfo
- Publication number
- CN115337219A CN115337219A CN202211104373.4A CN202211104373A CN115337219A CN 115337219 A CN115337219 A CN 115337219A CN 202211104373 A CN202211104373 A CN 202211104373A CN 115337219 A CN115337219 A CN 115337219A
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- skin
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Links
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- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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Abstract
The invention relates to a skin repair composition which is characterized by being prepared from the following raw materials in parts by mass: 0.05 part to 10 parts of trehalose, 0.1 part to 10 parts of polyacrylate, 1 part to 10 parts of humectant, 0.5 part to 10 parts of macromolecular polysaccharide, 0.5 part to 8 parts of emulsifier, 1.5 part to 30 parts of grease and 60 parts to 90 parts of water. The skin repairing composition disclosed by the invention has good stability at both high temperature and low temperature, and animal experiments prove that the skin repairing composition disclosed by the invention has a good skin repairing effect.
Description
Technical Field
The invention belongs to the field of skin care products, and particularly relates to a skin repair composition and a preparation method thereof.
Background
With the huge transformation of social concepts and the establishment of new medical models, minimally invasive shaping in medical beauty has gradually become a trend. According to the analysis report of network attention degree of medical and American industry at 2020, the medical and American minimally invasive plastic surgery occupies 38% of the total income of the plastic industry with the advantages of relatively low risk, short recovery period and the like. According to the advanced research report of Chinese micro-plastic industry in 2019, the top five items most popular with consumers in the micro-plastic service are respectively as follows: botulinum toxin injection, hyaluronic acid injection, laser depilation, autologous fat filling, and photon skin rejuvenation. However, the skin barrier may be damaged after minimally invasive surgery, and symptoms such as redness, swelling, heat, pain and the like may appear, which bothers people who love beauty.
Compared with non-sterile skin care product preparations, the sterile skin care product preparation has low biological load and higher safety. However, most of the substrates in the current market are water-soluble substrates in the sterile formula, and few reports are made on the emulsified substrates. For example, patent CN113975446A discloses a medical aseptic patch formula and a manufacturing method thereof, which comprises the following raw materials: 0.1-0.3% of main material, 0.1-0.5% of adhesive, 2-10% of humectant, 0.01-0.2% of preservative, 1-5% of bactericide and the balance of purified water. The patent provides a formula of a water-soluble matrix, and the product is lack of grease, poor in moisturizing effect and poor in repairing effect; in addition, the patent can not achieve good sterile effect only by adding a preservative and a bactericide to control the microbial load.
According to the characteristics and application of the moisturizing agent for cosmetics in the literature, the function of restoring the skin barrier is realized by adding the moisturizing agent, the grease and the skin barrier restoring component from three aspects of water replenishing, moisture preserving and restoring. The existing skin barrier repair products mainly achieve functions by adding a humectant and a repair component, and according to the literature 'research on influence of common oil on the moisturizing effect of cosmetics', due to lack of oil components, the moisturizing effect of the products is often poor. In addition to the addition of preservatives to inhibit microbial growth, further methods of killing microorganisms commonly used in the prior art include moist heat sterilization, radiation sterilization, and the like. Generally, the temperature required by moist heat sterilization is more than 115 ℃, the duration is long, and common cosmetic production equipment cannot meet the requirement; for radiation sterilization, a plurality of components in the skin care product are easy to change color, smell stimulation, extreme pH value, material body property change and the like after being irradiated, the irradiation is a strong oxidation process, the stability of the product can be damaged, and for an emulsion type matrix product, the low-temperature stability and the high-temperature stability of the product can be verified to be layered after being irradiated, so that the development of a sterile preparation of the emulsion type matrix is hindered.
In order to solve the problems in the prior art, the development of a skin care product which is sterile, has good stability and has excellent skin repairing function is urgently needed.
Disclosure of Invention
In view of the above-mentioned drawbacks and problems of the prior art, the present inventors have developed an emulsion-based, sterile skin repair composition and a method for preparing the same.
The invention provides a skin repair composition, which is characterized by being prepared from the following raw materials in parts by mass: 0.05 part to 10 parts of trehalose, 0.1 part to 10 parts of polyacrylate, 1 part to 10 parts of humectant, 0.5 part to 10 parts of macromolecular polysaccharide, 0.5 part to 8 parts of emulsifier, 1.5 part to 30 parts of grease and 60 parts to 90 parts of water.
Optionally, the macromolecular polysaccharide is selected from one or more of xanthan gum, sodium alginate and soluble starch; preferably, the macromolecular polysaccharide is a composition of 0.3-2.0 parts of soluble starch and 0-1.3 parts of sodium alginate or a composition of 0.3-2.0 parts of soluble starch and 0-0.8 part of xanthan gum.
Preferably, the components of the macromolecular polysaccharide can be the following combinations:
(1) 1.0-2.0 parts of soluble starch;
(2) 0.5-1.5 parts of soluble starch and 0.3-0.8 part of xanthan gum;
(3) 0.3-1.5 parts of soluble starch and 0.3-1.3 parts of sodium alginate.
More preferably, the components of the macromolecular polysaccharide can be the following combinations:
(1) 0.5-1.3 parts of soluble starch and 0.3-0.8 part of xanthan gum;
(2) 0.3-1.3 parts of soluble starch and 0.3-1.3 parts of sodium alginate.
Optionally, the emulsifier is selected from one or more of fatty acid monoglyceride, polyethylene glycol-7 stearate.
Optionally, the composition further comprises 0.1-2 parts of a neutralizing agent, preferably, the neutralizing agent is selected from one or more of sodium hydroxide and triethanolamine.
Optionally, the oil is selected from one or more of isopropyl myristate, vaseline, fatty alcohol, paraffin, fatty acid, and silicone oil; preferably, the grease is a composition of 0.5-10 parts of isopropyl myristate, 0.5-5 parts of vaseline, 0.5-5 parts of fatty alcohol and 1-10 parts of paraffin; more preferably, the fatty alcohol is a solid fatty alcohol. The solid fatty alcohol can be cetyl alcohol, stearyl alcohol, and cetostearyl alcohol for moisturizing cosmetic.
Optionally, the humectant is selected from one or more of glycerin, butylene glycol and propylene glycol.
Optionally, the composition further comprises 0.01-1 part of preservative, preferably, the preservative is selected from one or more of methyl hydroxybenzoate, hexanediol, phenoxyethanol and ethylhexyl glycerol.
The invention also provides a preparation method of the skin repairing composition, which is characterized by comprising the following steps:
(1) Weighing trehalose, polyacrylate, humectant, water and macromolecular polysaccharide according to the weight parts, heating to 70-90 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing the grease according to the weight part, heating to 60-90 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring the mixture to be uniform under the condition of heat preservation;
(4) Cooling to 30-55 deg.C, discharging, and packaging;
(5) And after filling, performing Co60 irradiation sterilization to obtain the skin repairing composition. Preferably, the Co60 radiation has an intensity ranging from 5 to 40Kgy.
Preferably, in the step (4), the temperature is reduced to 30-55 ℃, the neutralizer is weighed according to the weight part, added into the mixture obtained in the step (3), stirred uniformly, discharged and filled.
Optionally, the step (1) and/or (4) further comprises weighing and adding a preservative according to parts by weight.
The invention also provides the application of the skin repairing composition in preparing cosmetics, skin care products, medicines or medical devices for repairing the damaged skin barrier; preferably, the skin barrier damage is post-art skin barrier damage. Correspondingly, the invention also provides the application of the skin repair composition to treating the skin barrier damage.
The skin repair composition is an emulsified substrate, and has good moisturizing and skin barrier repair effects; the composition has good aseptic characteristics through Co60 irradiation sterilization; and the inventor finds the most appropriate component and proportion through a large number of experiments, and solves the problem that the emulsified matrix is unstable and easy to separate under irradiation. Experiments prove that the skin repairing composition has good stability and skin feel at high temperature and low temperature, animal experiments prove that the skin repairing composition has good skin repairing effect, and compared with similar products, the skin repairing composition has better effects in the aspects of safety and effectiveness.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
FIG. 1 is a schematic diagram of the distribution of experimental groups in the back experimental area of experimental rabbits.
FIG. 2 shows comparative results of rabbit skin repair tests.
FIG. 3 shows pathological HE results of rabbit skin repair test.
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be purely exemplary of the invention and are not intended to be limiting.
Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in experimental or practical applications, the materials and methods are described below. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
The test methods, detection methods and preparation methods of conventional experimental reagents used in the examples of the present invention were performed according to the routine procedures in the art, unless otherwise specified.
| Raw material | Rank of | Manufacturer of the product |
| Purified water | Conforming to the pharmacopoeia 2020 edition | Company self-made |
| Soluble starch | Pharmaceutical adjuvant | SHANDONG LIAOCHENG EHUA PHARMACEUTICAL Co.,Ltd. |
| Sodium alginate | Pharmaceutical adjuvant | QINGDAO HYZLIN BIOLOGY DEVELOPMENT Co.,Ltd. |
| Trehalose | Pharmaceutical adjuvant | JIANGSU DONGNAN NANO MATERIAL Co.,Ltd. |
| Glycerol | Pharmaceutical adjuvant | Hunan Er-Kang Pharmaceutical Co.,Ltd. |
| Xanthan gum | Pharmaceutical adjuvant | CP Kelco U.S.,Inc. |
| Hydroxy phenyl methyl ester | Pharmaceutical adjuvant | JIANGXI ALPHA HI-TECH PHARMACEUTICAL Co.,Ltd. |
| Polyethylene glycol-7 stearate | Pharmaceutical adjuvant | Gattefosse SAS |
| Paraffin wax | Medicinal auxiliary material | Hunan Er-Kang Pharmaceutical Co.,Ltd. |
| White vaseline | Pharmaceutical adjuvant | NANCHANG BAIYUN PHARMACEUTICAL Co.,Ltd. |
| Hexadecanol and octadecanol | Pharmaceutical adjuvant | Hunan Er-Kang Pharmaceutical Co.,Ltd. |
| Myristic acid isopropyl ester | Medicinal auxiliary material | Zhejiang Wumei Biotechnology Co.,Ltd. |
| Sodium hydroxide (NaOH) | Pharmaceutical adjuvant | Chengdu Mali-Rou pharmaceutical adjuvant manufacturing Limited liability company |
| Hexanediol | Pharmaceutical adjuvant | Finar Limited |
Example 1
The embodiment provides a skin repair composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin, 0.8g of a neutralizing agent (sodium hydroxide), 0.03g of trehalose and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate and purified water according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 was irradiated for sterilization to obtain the skin repair composition.
Example 2
The embodiment provides a skin repair composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); trehalose 0.05g, purified water 74g.
The preparation method is the same as in example 1.
Example 3
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of hexadecadecanol, 3g of paraffin and 0.8g of neutralizer (sodium hydroxide); trehalose 0.08g and purified water 74g.
The preparation method is the same as that of example 1.
Example 4
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); trehalose 0.1g, purified water 74g.
The preparation method is the same as in example 1.
Example 5
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1.5g of soluble starch and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water and soluble starch according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min under the condition of heat preservation until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 was irradiated for sterilization to obtain the skin repair composition.
Example 6
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1.5g of sodium alginate and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water and sodium alginate according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 was irradiated for sterilization to obtain the skin repair composition.
Example 7
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1.5g of xanthan gum and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water and xanthan gum according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 is irradiated for sterilization, and the skin repairing composition is obtained.
Example 8
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1g of soluble starch, 0.5g of sodium alginate and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water, soluble starch and sodium alginate according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 was irradiated for sterilization to obtain the skin repair composition.
Example 9
The embodiment provides a skin repair composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of hexadecadecanol, 3g of paraffin and 0.8g of neutralizer (sodium hydroxide); 0.05g of trehalose, 1g of soluble starch, 0.5g of xanthan gum and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water, soluble starch and xanthan gum according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 is irradiated for sterilization, and the skin repairing composition is obtained.
Example 10
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1g of sodium alginate, 0.5g of xanthan gum and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water, sodium alginate and xanthan gum according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 was irradiated for sterilization to obtain the skin repair composition.
Example 11
The embodiment provides a skin repair composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1g of sodium alginate, 0.5g of soluble starch and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water, sodium alginate and soluble starch according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 was irradiated for sterilization to obtain the skin repair composition.
Example 12
This example provides a skin rejuvenation composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1g of xanthan gum, 0.5g of soluble starch and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methyl hydroxybenzoate, purified water, xanthan gum and soluble starch according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 is irradiated for sterilization, and the skin repairing composition is obtained.
Example 13
The embodiment provides a skin repair composition, which comprises the following raw materials: 0.4g of sodium polyacrylate, 5g of glycerol, 0.1g of methylparaben, 0.7g of hexanediol, 4g of polyethylene glycol-7 stearate, 7g of isopropyl myristate, 3g of white vaseline, 2g of cetostearyl alcohol, 3g of paraffin and 0.8g of a neutralizing agent (sodium hydroxide); 0.05g of trehalose, 1g of xanthan gum, 0.5g of sodium alginate and 74g of purified water.
The preparation method comprises the following steps:
(1) Weighing trehalose, sodium polyacrylate, glycerol, methylparaben, purified water, xanthan gum and sodium alginate according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing polyethylene glycol-7 stearate, cetostearyl alcohol, isopropyl myristate, white vaseline and paraffin according to the weight, heating to 80 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring for 20min at the constant temperature until the mixture is uniform;
(4) Cooling to 50 deg.C, weighing sodium hydroxide and hexanediol according to the above weight, stirring for 10min, discharging, and bottling;
(5) After filling, 25Kgy Co60 is irradiated for sterilization, and the skin repairing composition is obtained.
Experimental example 1 Low and high temperature stability test
The experimental method comprises the following steps: the compositions obtained in examples 1 to 4 were allowed to stand at-18 ℃ and 45 ℃ for 30 days, respectively, and after returning to room temperature, the appearance was observed and recorded. The results are shown in table 1 below.
TABLE 1
Through the above experiments, researchers found that: after addition of a certain amount of trehalose, the low temperature stability problem of the composition was solved, but the high temperature stability was stratified. In order to solve the problem of high-temperature stability, researchers further add macromolecular polysaccharides of different types and amounts in the formula to verify the high-temperature stability of the product and verify the use feeling of the product. The results are shown in table 2 below.
TABLE 2
As can be seen from the above data, examples 8, 9 and 11 have good high temperature stability and give good skin feel. Therefore, 1 part of soluble starch, 0.5 part of xanthan gum or 1 part of soluble starch, 0.5 part of sodium alginate or 1 part of sodium alginate and 0.5 part of soluble starch are selected as the preferable formula of the invention.
EXAMPLE 2 effectiveness test (animal experiment)
The experimental animal source is as follows: woduoshuo laboratory animals Co Ltd
(1) Experiment grouping
Removing hair from the back of the rabbit, washing the part with warm water, and wiping the cotton ball for later use. The depilatory part is divided into six areas with the size of 2cm multiplied by 2cm in a left-right symmetry way: six regions 1#, 2#, 3#, 4#, 5#, and 6#. Of the six zones, the upper left is trial 1 (trial-example 8) with code # 1; in the left middle is test group 2 (test group-example 9) with code # 2; lower left trial 3 (trial-example 11) with code # 3; the upper right part is a control group of similar products (sodium alginate dressing, brand is Anjia), and its code is # 4; the right middle is a negative control group (physiological saline) with the code number of 5#; the blank control (no treatment) is shown at the bottom right and has the code number 6#. The specific location is shown in fig. 1.
(2) Test modeling and treatment method
Rabbits were anesthetized with 2.5% sodium pentobarbital at lmL/kg along the marginal ear vein. The micro-needle is used for carrying out the wound creation in five areas of No. 1, no. 2, no. 3, no. 4 and No. 5, the micro-needle frequency is 300 times/min, the single treatment lasts for 30s, and the treatment lasts for 3-5 times in total until the skin of the rabbit shows more serious erythema and forms shallow scars.
According to technical specifications (draft) of traditional Chinese medicine external pharmacological experiments, the powder or the paste is uniformly coated on a layer of the administration area, and the thickness is about 1-2 mm; the liquid can be directly applied by wet compress, and gauze is selected to quantitatively absorb the liquid. The sample is directly smeared on the area of the test group to completely cover the wound surface of the area, and the area of the negative control group is covered after the sterile gauze is soaked by sterile normal saline. The test site was covered with a bandage for at least 4h, the gauze piece and bandage were removed, the contact site was marked with permanent ink, and the residual test material was rinsed off with warm water and wiped dry. All areas were dosed 2 times a day (once in the morning and once in the evening) for 7 consecutive days, and the repaired skin was photographed and sampled on days 3 and 7 of successful molding.
(3) Evaluation method
(1) Skin surface observation: after the repairing and nursing, the rabbit skin surface micro-damage and the superficial scar tissue formation condition are observed by naked eyes.
Effect determination criteria: the effect is shown: macroscopic erythema, scar area regression >50%, improvement: macroscopic erythema, regression of scar area >30%, no effect: the macroscopic erythema and scar area regress by less than 30%. The total effective rate is calculated by obvious effect and improvement.
The statistical method comprises the following steps: adopting a chi type 2 Testing and analysis of variance, statistical analysis using SPSS software.
(2) Taking wound surface tissues for pathological HE examination: randomly extracting a rabbit, and respectively taking the wound surface tissues of a test group, a similar product control group and a negative control group on the 3 rd day to perform pathological HE (human attachment syndrome) examination; randomly selecting one rabbit, and taking the wound surface tissues of the test group, the control group of the similar product and the negative control group respectively on the 7 th day to perform pathological HE examination.
(4) Results of animal experiments
(1) Skin surface observation
The comparative results of the rabbit skin repair test are shown in the figure 2, the table 3 and the figure 3.
TABLE 3 evaluation table for rabbit skin repairing effect
The observation and statistics of the test results show that the area of the experimental group-example 8 has 22 cases of obvious effect, 8 cases of improvement and 0 case of no effect; the experimental group-example 9 area was used to show 22 cases, 8 improved cases and 0 invalid cases; the experimental group-example 11 area was used to show 27 cases, improved 3 cases and ineffective 0 case; the control group (sodium alginate dressing) of the similar product has 20 cases of obvious effect, 7 cases of improvement and 3 cases of no effect; the negative control area (normal saline) showed 4 cases, 5 improved cases and 21 ineffective cases; the use of the product was significantly different from the use of physiological saline (P < 0.01). By observing the repair condition of the wound surface, the erythema of the wound surface is obviously reduced after the product is continuously used for 7 days, and the normal skin structure of the wound surface area is basically recovered.
And (3) histological observation: pathological HE results of the rabbit skin repair test are shown in FIG. 3.
According to the HE pathological examination, the epidermis of the test group is discontinuous and has a clear structure, and inflammatory cells infiltrate into the epidermis on the 3 rd day; the epidermis of the normal saline group is discontinuous and has an unclear structure, and more inflammatory cells infiltrate into the epidermis; the similar product (sodium alginate dressing) has discontinuous epidermis and inflammatory cell infiltration in the epidermis. On day 7, the surface of the test group was restored to normal skin structure, while the epidermis of the similar product (sodium alginate dressing) group was still infiltrated with inflammatory cells.
To summarize: the observation of the repair condition of the wound surface shows that the erythema of the wound surface of the product is obviously reduced, the superficial scars are obviously reduced, and the normal skin structure of the wound surface area is basically recovered after the product is continuously used for 7 days, which indicates that the product has no stimulation effect on the animal skin, and can provide a microenvironment for the wound surface healing by forming a protective layer on the surface of the wound surface, effectively protect the wound surface and promote the wound surface healing recovery. Compared with the similar products (such as sodium alginate dressing), the product of the invention has better efficacy in the aspects of safety and effectiveness.
It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.
Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are also encompassed by the appended claims.
Claims (10)
1. The skin repair composition is characterized by being prepared from the following raw materials in parts by mass: 0.05 part to 10 parts of trehalose, 0.1 part to 10 parts of polyacrylate, 1 part to 10 parts of humectant, 0.5 part to 10 parts of macromolecular polysaccharide, 0.5 part to 8 parts of emulsifier, 1.5 part to 30 parts of grease and 60 parts to 90 parts of water.
2. The skin rejuvenation composition as defined in claim 1 wherein said macromolecular polysaccharide is selected from the group consisting of one or more of xanthan gum, sodium alginate, soluble starch; preferably, the macromolecular polysaccharide is a composition of 0.3-2.0 parts of soluble starch and 0-1.3 parts of sodium alginate or a composition of 0.3-2.0 parts of soluble starch and 0-0.8 parts of xanthan gum.
3. The skin rejuvenation composition as defined in claim 1 wherein said emulsifier is selected from one or more of the group consisting of fatty acid monoglycerides, polyethylene glycol-7 stearate.
4. The skin rejuvenation composition as defined in claim 1 further including a neutralizing agent in the range of from 0.1 parts to 2 parts, preferably said neutralizing agent is selected from one or more of sodium hydroxide and triethanolamine.
5. The skin rejuvenation composition as defined in claim 1 wherein said oil is selected from one or more of isopropyl myristate, petrolatum, fatty alcohols, paraffin, fatty acids, silicone oils; preferably, the grease is a composition of 0.5-10 parts of isopropyl myristate, 0.5-5 parts of vaseline, 0.5-5 parts of fatty alcohol and 1-10 parts of paraffin; more preferably, the fatty alcohol is a solid fatty alcohol.
6. The skin rejuvenation composition as defined in claim 1 wherein said humectant is selected from one or more of glycerin, butylene glycol and propylene glycol.
7. The skin rejuvenation composition as defined in claim 1 further including a preservative from 0.01 parts to 1 part, preferably said preservative is selected from one or more of methylparaben, hexylene glycol, phenoxyethanol, and ethylhexyl glycerin.
8. A method of preparing a skin rejuvenating composition as defined in claim 1 which comprises the steps of:
(1) Weighing trehalose, polyacrylate, humectant, water and macromolecular polysaccharide according to the weight parts, heating to 70-90 ℃, and uniformly stirring to obtain a mixture 1;
(2) Weighing the grease according to the parts by weight, heating to 60-90 ℃, and uniformly stirring to obtain a mixture 2;
(3) Adding the mixture 2 into the mixture 1, and stirring the mixture to be uniform under the condition of heat preservation;
(4) Cooling to 30-55 deg.C, discharging, and packaging;
(5) After filling, co60 is irradiated for sterilization to obtain the skin repairing composition;
preferably, in the step (4), the temperature is reduced to 30-55 ℃, the neutralizing agent is weighed according to the weight part, added into the mixture obtained in the step (3), stirred uniformly, discharged and filled.
9. The method for preparing a skin rejuvenation composition as defined in claim 8 wherein steps (1) and/or (4) further include weighing in parts by weight and adding a preservative.
10. Use of a skin repair composition according to any one of claims 1 to 7 in the manufacture of a cosmetic, skin care, pharmaceutical or medical device for repairing a compromised skin barrier; preferably, the skin barrier damage is post-art skin barrier damage.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107375012A (en) * | 2017-08-11 | 2017-11-24 | 天津嘉氏堂科技有限公司 | A kind of skin barrier remediation composition and preparation |
| CN107375032A (en) * | 2017-08-11 | 2017-11-24 | 天津嘉氏堂科技有限公司 | A kind of composition and preparation method for repairing skin barrier |
| CN107412255A (en) * | 2017-08-11 | 2017-12-01 | 天津嘉氏堂科技有限公司 | Skin barrier remediation composition and preparation |
| CN110755328A (en) * | 2019-12-02 | 2020-02-07 | 中国林业科学研究院林产化学工业研究所 | Beautifying, moisturizing and repairing composition and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375012A (en) * | 2017-08-11 | 2017-11-24 | 天津嘉氏堂科技有限公司 | A kind of skin barrier remediation composition and preparation |
| CN107375032A (en) * | 2017-08-11 | 2017-11-24 | 天津嘉氏堂科技有限公司 | A kind of composition and preparation method for repairing skin barrier |
| CN107412255A (en) * | 2017-08-11 | 2017-12-01 | 天津嘉氏堂科技有限公司 | Skin barrier remediation composition and preparation |
| CN110755328A (en) * | 2019-12-02 | 2020-02-07 | 中国林业科学研究院林产化学工业研究所 | Beautifying, moisturizing and repairing composition and application thereof |
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