CN115287237B - Biocontrol bacteria for banded sclerotial blight of wheat, biocontrol preparation and application - Google Patents

Biocontrol bacteria for banded sclerotial blight of wheat, biocontrol preparation and application Download PDF

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CN115287237B
CN115287237B CN202211029288.6A CN202211029288A CN115287237B CN 115287237 B CN115287237 B CN 115287237B CN 202211029288 A CN202211029288 A CN 202211029288A CN 115287237 B CN115287237 B CN 115287237B
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biocontrol
wheat
preparation
sheath blight
microbial agent
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杨丽荣
徐文
谢夏
邓晓旭
张洁
夏明聪
孙润红
武超
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Institute of Plant Protection of Henan Academy of Agricultural Sciences
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Abstract

The invention belongs to the technical field of biological control, and discloses a biocontrol bacterium for wheat sheath blight, a biocontrol preparation and application. The preservation number of the biocontrol strain of the sheath blight of wheat is CGMCC No.24702. The biocontrol agent prepared by the invention can improve soil fertility while preventing and treating sheath blight, is environment-friendly, and can realize green prevention and control of crop diseases. Has obvious promoting effect on improving the yield and quality of wheat. The production cost is low, the international competitiveness of agricultural products can be improved, and the agricultural products are easy to popularize.

Description

Biocontrol bacteria for banded sclerotial blight of wheat, biocontrol preparation and application
Technical Field
The invention relates to the technical field of biological control, in particular to a biocontrol bacterium for wheat sheath blight, a biocontrol preparation and application.
Background
Rhizoctonia cerealis is caused by Rhizoctonia cerealis and the like, and is also called damping off and punctate. Sheath blight of wheat mainly occurs on leaf sheaths and stems. At the beginning of the disease, yellow brown oval or fusiform lesions are generated on leaf sheaths on the earth surface or near the earth surface, later the lesions gradually enlarge and become darker and develop into harmful stems inwards, and the lesions on leaf sheaths at the middle to late stages of wheat growth are in moire-like patterns. The rhizoctonia cerealis can damage tissue parts such as stalks of the wheat, influences the transportation of nutrient substances and moisture, causes the appearance of dry white ears, reduces the yield and quality of the wheat, and is one of factors restricting the high yield and stable yield of the wheat. Wheat sheath blight commonly occurs in wheat planting areas, is a common disease in wheat areas, has a disease-causing plant rate of 10% -30% in general disease fields, has a serious disease field block of 60% -80%, and has a particularly serious field block withered and whitehead rate caused by the disease of more than 20%. The yield loss caused by diseases is generally about 10 percent, and is as high as 30 to 40 percent in severe cases.
At present, the prevention and treatment means for the sheath blight of wheat mainly comprise agricultural planting prevention and treatment, chemical agent prevention and treatment, breeding of disease-resistant varieties, biological prevention and treatment and the like. The agricultural control needs to have higher grasp on details in the wheat planting process, and has poor control effect and is not easy to realize for vast farmers; chemical control can play a good role in control, but the application of chemical agents can pollute and destroy wheat plants and the surrounding growth environment. The bred disease-resistant varieties have fewer varieties with better effects, and the bred disease-resistant varieties have long period and unstable resistance. At present, due to the lack of natural resistance resources, the identified and developed disease resistance resources are limited, and the traditional breeding method cannot effectively control the harm of pathogenic bacteria. For a long time, the prevention and treatment of the sheath blight of wheat is mainly performed by using chemical bactericides, which causes serious environmental pollution and ecological environmental problems.
Biological control is receiving more and more attention and research because of its green, safe and efficient. In the prevention and treatment of the sheath blight of wheat, biological prevention and treatment bacteria which are safe to ecology and environment are needed to be screened, and basic physiological and biochemical properties, microbial inoculum research and development and the like are researched, so that a theoretical basis is provided for the biological prevention and treatment of the sheath blight of wheat.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a biocontrol bacterium for wheat sheath blight, a biocontrol preparation and application.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
in a first aspect, the invention provides a biocontrol bacterium for wheat sheath blight, which has a preservation number of CGMCC No.24702. The strain is named as Pseudomonas orientalis Pseudomonas orientalis YB-76, is preserved in China general microbiological culture Collection center (China Committee) for culture Collection of microorganisms in the year 2022, and has an address of North Chen Xili No. 1 and 3 in the Korean region of Beijing city.
In a second aspect, the invention provides a biocontrol microbial agent prepared from the wheat sharp eyespot biocontrol microbial agent.
As a preferred embodiment of the biocontrol microbial agent, the effective viable count of the biocontrol microbial agent for the sheath blight of wheat is 200-300 hundred million/g.
In a third aspect, the invention provides a preparation method of the biocontrol microbial agent, which comprises the following steps:
(1) Inoculating the biocontrol strain of the sheath blight of wheat in the claim 1 to a solid culture medium for culture to obtain a colony;
(2) Selecting bacterial colony to inoculate in the first seed culture medium for culturing to obtain first seed;
(3) Inoculating the primary seeds into a fermentation culture medium according to the inoculum size of 1-6% by volume, aerating, and culturing for 28-40 h to obtain secondary seeds;
(4) And (3) inoculating the secondary seeds into a fermentation tank according to the inoculum size of 1-6% by volume, and performing high-density fermentation culture to obtain the biocontrol microbial inoculum.
As a preferred embodiment of the production method of the present invention, in the step (1), the temperature of the culture is 26℃to 30℃for 28 hours to 40 hours; in the step (2), the initial pH value of the culture is 7.0-7.2, the temperature is 24-30 ℃ and the time is 20-24 hours; the formula of the primary seed culture medium is as follows: 10g of corn flour, 5g of glucose, 15g of bean cake powder, 5g of fish meal and CaCO 3 5g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O0.2 g, water 1000ml.
As a preferred mode of the preferred implementation of the preparation method of the present invention, in the step (3), the formulation of the secondary seed medium is:corn flour 12g, glucose 5g, bean cake powder 18g, fish meal 4g, caCO 3 6.50g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O0.2 g, water 1000ml.
In a fourth aspect, the invention provides a biocontrol agent, which comprises, by mass, 60-80 parts of the antibacterial agent, 7-10 parts of the adhesive, 1-2 parts of the stabilizer and 7-31 parts of the filler.
As a preferred embodiment of the biocontrol microbial agent, the adhesive is at least one of maltodextrin, soybean powder and gelatin; the stabilizer is bentonite and/or carboxymethyl cellulose; the filler is at least one of talcum powder, attapulgite, clay and white carbon black.
In a fifth aspect, the present invention provides a method for preparing the biocontrol agent, comprising the steps of:
(1) Mixing the biocontrol microbial agent with the filler, and spray drying to prepare mother powder;
(2) And adding the adhesive and the stabilizer into the mother powder.
In a sixth aspect, the invention applies the preparation methods of the biocontrol strain of wheat sheath blight, the biocontrol microbial inoculum and the biocontrol microbial inoculum in biological control of plant diseases caused by rhizoctonia cerealis and/or fusarium.
Compared with the prior art, the invention has the beneficial effects that:
the wheat sheath blight biocontrol bacterium has good stress resistance, can survive for a long time in some extreme environments, is favorable for preservation, and is very suitable for preservation, processing and application of microbial preparations; the prepared biocontrol agent can improve soil fertility, activate and improve soil while preventing banded sclerotial blight, is environment-friendly, can realize green prevention and control of crop diseases, can promote the quality safety of agricultural products and protect the ecological safety of agriculture, is beneficial to nuisanceless production of crops and is beneficial to improving the quality of crops; the later-stage grouting and thousand grain weight of the wheat can be improved, and the method has obvious promotion effect on improving the yield and quality of the wheat; the production cost is low, the international competitiveness of agricultural products can be improved, and the agricultural products are easy to popularize.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples. It will be appreciated by persons skilled in the art that the specific embodiments described herein are for purposes of illustration only and are not intended to be limiting.
The test methods used in the examples are conventional methods unless otherwise specified; the materials, reagents and the like used, unless otherwise specified, are all commercially available.
Example 1: strain screening and identification
1. Strain screening
Soil samples were collected from Liu Quanzhen wheat fields of Yiyang county, luoyang, henan province in 2020, and YB-76 strain was isolated from wheat root soil samples infected with sheath blight. The method comprises the following steps:
a dilution separation method is adopted. 1g of each soil sample is weighed and diluted to 10 by sterile water -5 Sucking 200 mu L of soil sample suspension, uniformly coating on an NA plate, placing in a 37 ℃ incubator for culturing for 30 hours, picking single colonies with different forms by using an inoculating loop, streaking and purifying on the NA plate, and preserving for later use after culturing.
Antagonistic strains were screened by plate confrontation. Inoculating a sheath blight germ cake with a diameter of 6mm at the center of a flat plate, culturing for 2 days in a constant temperature box at 25 ℃, picking a small amount of bacteria on two sides of a position 2.5cm away from the germ cake by using an inoculating needle, respectively drawing 2 parallel straight lines with a length of 3cm, placing the parallel straight lines in the constant temperature box at 25 ℃, continuously culturing for 3 days, observing the growth condition of the sheath blight germ, and measuring the colony diameter of the sheath blight germ if a bacteriostatic zone appears. Each treatment was repeated 3 times, and the relative inhibition was calculated using plates inoculated with sheath blight bacterial cake alone as a control group. From which the YB-76 strain was isolated.
2. Identification of strains
The screened strain is tested for morphological characteristics, physiological and biochemical characteristics, gram staining and other indexes, and the method is specifically referred to Cai Miaoying and Dongxiu beads. The method comprises the following steps:
morphological characteristics:
the YB-76 strain is streaked on NA solid medium, cultured overnight at 30 ℃, and the colony morphology and color are observed. The strain YB-76 has good growth state on NA culture medium, milky and semitransparent colony, smooth surface, regular edge and non-sticky picking.
Physiological and biochemical characteristics:
the physiological and biochemical identification results show that the strain YB-76 has positive protease activity, beta-1, 3-glucanase activity, siderophore production, gelatin hydrolysis, urease test and the like, and the cellulase activity, pectinase activity, amylase activity, xylanase activity, phosphorus dissolution, IAA production, ACC deaminase activity, chitinase activity, indole, lysine and the like have negative effects.
Gram staining:
gram stain negative, no spore, no capsule, rod-shaped or slightly bent cells with terminal flagella, and capable of movement. Based on the results of the cell morphological characteristics and physiological and biochemical characteristics, the YB-76 is primarily identified as Pseudomonas according to the Berger's bacteria identification handbook. Further molecular biological identification was performed as follows:
genomic DNA of strain YB-76 was extracted according to the instructions of TaKaRa MiniBEST Bacteria Genomic DNA Extraction Kit Ver.3.0 kit and the 16S rRNA gene sequence was amplified using bacterial universal primers 27-F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492-R (5'-GGTTACCTTGTTACGACTT-3') using the extracted genomic DNA as a template. The PCR products were sequenced by Shangya bioengineering (Shanghai) Inc., and the sequencing results were compared to the GenBank database in NCBI. The 16S rRNA sequence obtained by YB76 sequencing is compared with the 16S rRNA sequence of a known strain downloaded from a GenBank database in NCBI by using MUSCLE software in Mega 7.0 software, and then a phylogenetic tree of the strain YB-76 is constructed by adopting an NJ method, wherein the sampling frequency is 1000.
The 16S rRNA gene is obtained by taking the genome DNA of the strain YB-76 as a template for amplification, and the sequencing result is compared with the sequence in GenBank, so that the result shows that the similarity of YB-76 and Pseudomonas orientalis is 100 percent. The constructed phylogenetic tree shows that YB-76 is in the same branch of the phylogenetic tree as Pseudomonas orientalis. Therefore, the strain is named as Pseudomonas orientalis YB-76 and is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 24702 in the year of 2022, month 05 and 18.
Example 2: cultivation of biocontrol bacteria
The strain culture comprises the following steps:
(1) Culturing in a culture dish: inoculating Pseudomonas orientalis YB-76 on NA solid culture medium under aseptic condition, and culturing at 26-30deg.C, preferably 30deg.C for 28-40 hr;
(2) Primary seed culture: inoculating the strain cultured in the step (1) into a primary seed culture medium under aseptic condition, culturing for 20-24 hours at 24-30 ℃ and preferably 30 ℃ at an initial pH value of 7.0-7.2 to obtain primary seeds;
the formula of the first-stage seed culture medium is as follows: 10g of corn flour, 5g of glucose, 15g of bean cake powder, 5g of fish meal and CaCO 3 5g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O0.2 g, water 1000ml;
(3) Secondary seed culture: inoculating the primary seeds into a fermentation culture medium according to the inoculum size of 1-6% by volume, aerating, and culturing for 28-40 hours to obtain secondary seeds;
the formula of the secondary seed culture medium is as follows: corn flour 12g, glucose 5g, bean cake powder 18g, fish meal 4g, caCO 3 6.50g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O0.2 g, water 1000ml;
(4) Fermentation culture: inoculating the secondary seeds into a fermentation tank according to the inoculum size of 1-6% by volume percent, and performing high-density fermentation culture to obtain bacterial suspension of the strain;
(5) The effective viable count of the concentrated bacterial liquid is 200 hundred million/g.
Example 3: cultivation of biocontrol bacteria
The strain culture comprises the following steps:
(1) Culturing in a culture dish: inoculating Pseudomonas orientalis YB-76 on NA solid culture medium under aseptic condition, and culturing at 30deg.C for 48 hr;
(2) Primary seed culture: inoculating the strain cultured in the step (1) into a primary seed culture medium under the aseptic condition, wherein the initial pH value is 7.0, and culturing for 24 hours at 30 ℃ to obtain primary seeds;
the formula of the first-stage seed culture medium is as follows: 10g of corn flour, 5g of glucose, 15g of bean cake powder, 5g of fish meal and CaCO 3 5g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O0.2 g, water 1000ml;
(3) Secondary seed culture: inoculating the primary seeds into a fermentation culture medium according to the inoculation amount of 5% by volume, aerating, and culturing for 32 hours to obtain secondary seeds;
the formula of the secondary seed culture medium is as follows: corn flour 12g, glucose 5g, bean cake powder 18g, fish meal 4g, caCO 3 6.50g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O0.2 g, water 1000ml.
(4) Fermentation culture: inoculating the second-level seeds into a fermentation tank according to the inoculum size of 5% by volume, and performing high-density fermentation culture to obtain bacterial suspension of the strain;
(5) Concentrating until the effective viable count of the bacterial liquid is 200 hundred million/g, thus obtaining concentrated bacterial liquid.
Example 4: preparation of biocontrol agent
(1) Mixing the concentrated bacterial solution prepared in the example 3 with talcum powder and attapulgite, and spray-drying to prepare mother powder;
(2) Maltodextrin and bentonite are added into the mother powder to prepare the biocontrol agent.
Wherein the mass percentages of the concentrated bacterial liquid, the maltodextrin and the bentonite are 60%, 8% and 1%, and the talcum powder and the attapulgite complement 100%.
Example 5: preparation of biocontrol agent
(1) Mixing the concentrated bacterial solution prepared in the example 3 with clay, and spray drying to obtain mother powder;
(2) Soybean powder and bentonite are added into the mother powder to prepare the biocontrol agent.
Wherein the mass percentages of the concentrated bacterial liquid, the soybean powder and the bentonite are 70%, 9% and 1.5%, and the clay complement 100%.
Example 6: preparation of biocontrol agent
(1) Mixing the concentrated bacterial solution prepared in the example 3 with white carbon black, and spray drying to prepare mother powder;
(2) Adding gelatin and carboxymethyl cellulose into the mother powder to obtain microbial preparation.
Wherein the mass percentages of the concentrated bacterial liquid, the gelatin and the carboxymethyl cellulose are 80%, 10% and 2%, and the white carbon black is 100%.
Test example 1: application of biocontrol agent in potting
The test is carried out in a greenhouse of the academy of agricultural sciences of Henan province, and the matrix material used in the indoor potting experiment is turfy soil and vermiculite mixed. The test wheat variety is Zhengmai 366; the treatment method comprises the following steps:
(1) Inoculating a fungus cake: taking Rhizoctonia cerealis (stored in a molecular biology laboratory of a plant protection institute of academy of agricultural sciences, henan province) as a pathogen to be tested, transferring the pathogen to a PDA flat plate, punching the pathogen into a stipe with the diameter of 1cm by a puncher for standby, filling a matrix material into a nutrition pot, flattening the surface, placing the stipe on the matrix, allowing the bacteria to face upwards, sowing seeds with different treatments, placing one seed on each stipe, and covering a sowing layer with the matrix;
(2) Seed dressing treatment of biocontrol agent of example 4: sterilizing wheat seeds with 1% sodium hypochlorite for 10min, washing with sterile water for 3 times, drying in the shade, adding appropriate amount of clear water into microbial agent, stirring, and drying in the shade;
(3) Drug control: sterilizing wheat seeds for 10min by using 1% sodium hypochlorite, washing 3 times by using sterile water, drying in the shade, respectively using 24% validamycin aqueous agent and 430 g/l tebuconazole suspending agent to stir the seeds, and drying the surfaces of the seeds in the shade for later use;
(4) Control: wheat seeds are sterilized by 1% sodium hypochlorite for 10min, washed 3 times by sterile water and dried in the shade for standby.
Each treatment was repeated 4 times, 15 grains per pot, and root-washing investigation after 28d incubation at 25 ℃. And (5) calculating the investigation result after the control is completely ill.
Grading standard:
0: no symptoms;
1: the blastoderm segments turn brown;
2: the blastoderm segments, the middle stems or leaf pins turn brown;
3: the blastoderm segments, the middle stems or leaf pins are all brown;
4: the middle stems, leaf pins and lower stems turn brown;
5: the stem has symptoms of dry rot;
6: plant death caused by stalk rot;
7: seedling blight or seed rot.
The statistical results are shown in table 1:
TABLE 1 control effect on wheat sheath blight potted plants
Treatment of Morbidity/% Index of disease condition Control effect/%
Control treatment 75.00±2.06 42.90±1.49 --
Biocontrol agent treatment of example 4 22.33±1.75 9.57±0.67 77.57±1.90Aa
24% validamycin aqueous agent treatment 20.33±2.74 8.33±1.35 80.25±3.75Aa
Treatment of 430 g/L tebuconazole suspension 22.67±1.44 9.05±0.67 78.97±1.05Aa
Note that: the mean value of each treatment represents a difference of up to 5% significant level with different letters.
The experimental results (table 1) of potting prevention and treatment show that the biocontrol preparation of the embodiment 4 can prevent and treat the sheath blight of wheat, has a good prevention and treatment effect reaching 77.57 percent, and has no significant difference with the prevention and treatment effect of 24 percent validamycin aqueous agent and 430 g/l tebuconazole suspending agent on the sheath blight of wheat.
Test example 2: application of biocontrol agent in field
The test is set in the Henan agricultural academy of sciences test demonstration base, 30m per treatment 2 The seed dosage is 12.5Kg/667m 2 。
The test had 4 treatments: the biocontrol formulation of example 5, 3% difenoconazole seed suspension and commercially available biocontrol formulation (bacillus subtilis) and a blank control. Normal fertilization management, 1 day before sowing (sowing time is 2021, 10 months and 8 days), seed coating treatment, 27g of the biocontrol agent of the example 5 is mixed with 3.4kg of wheat, 8.4ml of difenoconazole suspension seed coating agent is mixed with 3.4kg of wheat, a proper amount of clear water is added during seed dressing, and the mixture is fully and uniformly stirred and then is placed in a ventilated place for drying in the shade for standby. 3% difenoconazole seed suspending agent is used for soaking seeds, and is placed in a ventilation place for drying in the shade for standby. Each treatment was repeated 4 times.
According to the industry standard published by the agricultural division of the people's republic of China (GB/T17980.108-2004) pesticide field efficacy test criterion (II), part 108: investigation of the control effect of the bactericide for controlling the sheath blight of wheat: the sheath blight disease occurrence condition of each cell is investigated 30 days after the last administration, and the investigation method is as follows: samples were taken at random 5 spots per cell, each spot investigating 100 strains. Morbidity is recorded. The specific classification method is as follows:
level 0: does not cause disease;
stage 1: leaf sheath onset but stem onset;
3 stages: leaf sheath onset and invasion of the stem, but the stem leaf spot ring stem is less than 1/2;
5 stages: the stem disease spot ring stem exceeds 1/2, but does not lodge or break;
7 stages: dead, lodging, and withered ears.
The investigation method is that five-point sampling is adopted in each district before harvesting, 1 meter double rows are investigated in each point, average row spacing (the test is unified in machine sowing, the average row spacing is 21 cm) is measured, and the wheat spike number is reduced to the mu spike number; and selecting representative 20 ears to calculate average ear grain number, drying, weighing thousand grains, and beating 85 to calculate theoretical yield.
The analysis of wheat yield increase effect is shown in Table 2:
table 2 wheat yield test results
Note that: the mean value of each treatment represents a difference of up to 5% significant level with different letters.
As can be seen from the data in table 2 of example 5, the biocontrol agent of example 5 can prevent and treat banded sclerotial blight of wheat, has a good prevention and treatment effect which reaches 76.32%, is obviously higher than the prevention and treatment effect of a commercial biocontrol agent (subtilis) on banded sclerotial blight of wheat, and has no obvious difference from the prevention and treatment effect of a 3% difenoconazole suspending agent serving as a control agent on banded sclerotial blight of wheat.
The effect difference of the 3 treatments on the wheat yield is obvious, especially the biocontrol agent has the best treatment effect, the yield increase rate is 26.2 percent, and the effect is obviously higher than that of other 3 percent difenoconazole and the commercial biocontrol agent (bacillus subtilis) treatment. 3% difenoconazole is applied, and the yield increase is 21.7%. From this data, the biocontrol agent of example 5 has not only control effect but also yield increasing effect, superior to other chemical agents.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.

Claims (10)

1. The biocontrol bacterium for the sheath blight of wheat is characterized in that the preservation number is CGMCC No.24702.
2. A biocontrol microbial agent prepared from the wheat sharp eyespot biocontrol fungus of claim 1.
3. The biocontrol microbial agent of claim 2, wherein the effective viable count of said wheat sharp eyespot biocontrol bacteria is 200-300 hundred million/gram.
4. A method for preparing the biocontrol microbial agent as claimed in claim 2, comprising the steps of:
(1) Inoculating the biocontrol strain of the sheath blight of wheat in the claim 1 to a solid culture medium for culture to obtain a colony;
(2) Selecting bacterial colony to inoculate in the first seed culture medium for culturing to obtain first seed;
(3) Inoculating the primary seeds into a fermentation culture medium according to the inoculum size of 1-6% by volume, aerating, and culturing for 28-40 h to obtain secondary seeds;
(4) And (3) inoculating the secondary seeds into a fermentation tank according to the inoculum size of 1-6% by volume, and performing high-density fermentation culture to obtain the biocontrol microbial inoculum.
5. The method according to claim 4, wherein in the step (1), the temperature of the culture is 26℃to 30℃for 28 hours to 40 hours; in the step (2), the initial pH value of the culture is 7.0-7.2, the temperature is 24-30 ℃ and the time is 20-24 hours; the formula of the primary seed culture medium is as follows: 10g of corn flour, 5g of glucose, 15g of bean cake powder, 5g of fish meal and CaCO 3 5g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O0.2 g, water 1000ml.
6. The method of claim 4, wherein in step (3), the secondary seed medium is formulated as follows: corn flour 12g, glucose 5g, bean cake powder 18g, fish meal 4g, caCO 3 6.50g，(NH 4 ) 2 SO 4 1g，K 2 HPO 4 0.3g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O0.2 g, water 1000ml.
7. The biocontrol preparation is characterized by comprising, by mass, 60-80 parts of the biocontrol microbial agent of claim 2, 7-10 parts of an adhesive, 1-2 parts of a stabilizer and 7-31 parts of a filler.
8. The biocontrol microbial agent of claim 7, wherein said adhesive is at least one of maltodextrin, soy flour, gelatin; the stabilizer is bentonite and/or carboxymethyl cellulose; the filler is at least one of talcum powder, attapulgite, clay and white carbon black.
9. A method of preparing the biocontrol formulation of claim 7, comprising the steps of:
(1) Mixing the biocontrol microbial agent with the filler, and spray drying to prepare mother powder;
(2) And adding the adhesive and the stabilizer into the mother powder.
10. The biocontrol bacteria for wheat sheath blight according to claim 1, the biocontrol bacteria according to claim 2 or 3, the preparation method of the biocontrol bacteria according to any one of claims 4 to 6, the biocontrol preparation according to claim 7 or 8, and the application of the preparation method of the biocontrol preparation according to claim 9 in the biological control of plant diseases caused by rhizoctonia cerealis.
CN202211029288.6A 2022-08-25 2022-08-25 Biocontrol bacteria for banded sclerotial blight of wheat, biocontrol preparation and application Active CN115287237B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105316258A (en) * 2015-10-26 2016-02-10 江苏省安丰生物源农药工程中心有限公司 Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2
CN105754909A (en) * 2016-04-28 2016-07-13 韩山师范学院 Endophytic antagonistic pseudomonas ZF02 in Fenghuang-Dancong tea plants and application thereof to biocontrol
CN112899205A (en) * 2021-03-31 2021-06-04 慕恩（广州）生物科技有限公司 Pseudomonas chlororaphis MN225969 and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105316258A (en) * 2015-10-26 2016-02-10 江苏省安丰生物源农药工程中心有限公司 Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2
CN105754909A (en) * 2016-04-28 2016-07-13 韩山师范学院 Endophytic antagonistic pseudomonas ZF02 in Fenghuang-Dancong tea plants and application thereof to biocontrol
CN112899205A (en) * 2021-03-31 2021-06-04 慕恩（广州）生物科技有限公司 Pseudomonas chlororaphis MN225969 and application thereof

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