CN115165975B - Environment-friendly animal bacteria detection device and detection method thereof - Google Patents
Environment-friendly animal bacteria detection device and detection method thereof Download PDFInfo
- Publication number
- CN115165975B CN115165975B CN202210897135.7A CN202210897135A CN115165975B CN 115165975 B CN115165975 B CN 115165975B CN 202210897135 A CN202210897135 A CN 202210897135A CN 115165975 B CN115165975 B CN 115165975B
- Authority
- CN
- China
- Prior art keywords
- detection
- solution
- liquid
- communicated
- negative pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 150
- 241000894006 Bacteria Species 0.000 title claims abstract description 69
- 239000007788 liquid Substances 0.000 claims abstract description 134
- 230000001580 bacterial effect Effects 0.000 claims abstract description 37
- 239000002699 waste material Substances 0.000 claims abstract description 4
- 238000004064 recycling Methods 0.000 claims abstract description 3
- 239000002184 metal Substances 0.000 claims description 11
- 239000000523 sample Substances 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 78
- 238000010586 diagram Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005096 rolling process Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- UHZZMRAGKVHANO-UHFFFAOYSA-M chlormequat chloride Chemical compound [Cl-].C[N+](C)(C)CCCl UHZZMRAGKVHANO-UHFFFAOYSA-M 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
- G01N27/08—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid which is flowing continuously
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The invention relates to the technical field of bacteria detection and discloses an environment-friendly animal bacteria detection device and a detection method thereof, wherein the detection device comprises a bacteria detection module, a solution storage cavity communicated with the bacteria detection module and an impedance analyzer electrically connected with the bacteria detection module; the solution storage cavity comprises a first liquid storage cavity for supplying liquid to the bacteria detection module and a second liquid storage cavity for recycling waste liquid; the first liquid storage cavity comprises a first liquid supply cavity which is communicated with the bacteria detection module and used for storing a bacterial solution, and a second liquid supply cavity which is communicated with the bacteria detection module and used for storing a high-conductivity solution; the detection method comprises the following steps: s1, placing a solution; s2, matching and adjusting the flow rate; s3, detecting micro-flow; the invention can improve the stability of the flow velocity of the liquid in the micro-flow channel and ensure the accurate detection of the bacterial solution.
Description
Technical Field
The invention relates to the technical field of bacteria detection, in particular to an environment-friendly animal bacteria detection device and a detection method thereof.
Background
The conventional detection method of bacteria mainly comprises culturing and identifying bacteria, specifically culturing a bacterial sample by using a culture dish, and then identifying by using an electronic instrument; the method has long period, harsh culture conditions, difficult rapid detection, low safety, easy pollution to the environment and unfavorable environmental protection.
For detecting animal bacteria, the etiology is generally detected, and after samples such as animal blood and secretion are selected, bacteria culture is carried out or a smear is used for finding bacteria under a microscope, so that long detection time is required, and rapid detection cannot be carried out.
Therefore, the prior art develops a plurality of bacteria detection methods based on micro-fluidic chips; the micro-fluidic chip technology is a technology for controlling micro-fluid in a micro-channel and controlling the fluid in the micro-channel by a micro-sample injection technology to implement bacteria detection; when bacteria liquid passes through the detection section through negative pressure suction, different pulse signals are generated according to the size and surface properties of bacteria, and the pulse signals can be converted into relevant information such as the number and the type of bacteria after amplification and sorting and accumulation recording.
The prior art cannot perform better control on the fluid in the microfluidic channel, so that the internal flow speed stability of the microfluidic channel provided by the prior art is poor, and detection errors are easily caused.
Disclosure of Invention
The invention solves the technical problems that: the micro-flow control device for bacteria detection is provided, the stability of the liquid flow velocity in the micro-flow channel is improved, and accurate bacteria detection is ensured.
The technical scheme of the invention is as follows: an environment-friendly animal bacteria detection device comprises a bacteria detection module, a solution storage cavity communicated with the bacteria detection module, and an impedance analyzer electrically connected with the bacteria detection module;
The solution storage cavity comprises a first liquid storage cavity for supplying liquid to the bacteria detection module and a second liquid storage cavity for recycling waste liquid;
the first liquid storage cavity comprises a first liquid supply cavity which is communicated with the bacteria detection module and used for storing a bacterial solution, and a second liquid supply cavity which is communicated with the bacteria detection module and used for storing a high-conductivity solution;
The bacteria detection module comprises a flow rate regulator communicated with the first liquid supply cavity and the second liquid supply cavity, a support frame arranged on the flow rate regulator, and a multichannel detector arranged on the support frame and communicated with the flow rate regulator and the second liquid storage cavity;
The flow speed regulator comprises a first main board, first-order regulators which are uniformly arranged on the first main board and are communicated with the first liquid supply cavity and the second liquid supply cavity, and second-order regulators which are uniformly arranged on the first main board and are communicated with the first-order regulators at one end;
the multichannel detector comprises a second main board and a plurality of micro-flow detection channels which are uniformly arranged on the second main board and are correspondingly communicated with the other ends of the second-order regulators respectively;
The first-order regulator comprises a protection box arranged on the first main board, a first negative pressure device arranged in the protection box and communicated with the first liquid supply cavity and the detection channel, and a second negative pressure device arranged in the protection box and communicated with the second liquid supply cavity and the micro-flow detection channel;
The second-order regulator comprises a conveying belt which is arranged on the first main board, one end of the conveying belt is simultaneously communicated with the first negative pressure device and the second negative pressure device, a clamping assembly which is movably arranged on the conveying belt, and a power device which is arranged on the first main board and used for driving the clamping assembly;
The conveyer belt comprises a flat flexible conveyer belt body, and two wear-resistant metal sheets respectively and correspondingly paved on the surface of the flexible conveyer belt body;
three pipelines are arranged in the flexible conveying belt body side by side; the central pipeline is used for conveying the bacterial solution, and pipelines on two sides of the central pipeline are used for conveying the high-conductivity solution;
The clamping assembly clamps the wear-resistant metal sheet and can slide on the wear-resistant metal sheet along the extending direction of the flexible conveying belt body.
Further, the microfluidic detection channel comprises an installation box arranged on the second main board, a detection section arranged in the middle of the installation box, and a liquid inlet component and a liquid outlet component which are arranged in the installation box and are respectively positioned at the end parts of the detection section;
One end of the mounting box is provided with a liquid inlet clamping socket for communicating the liquid inlet assembly with the conveying belt; the other end of the mounting box is provided with a liquid discharge clamping socket for communicating the liquid discharge assembly with the second liquid storage cavity;
the liquid inlet assembly comprises a liquid inlet micro pipe, a pipeline support piece and a liquid inlet control unit, wherein one end of the liquid inlet micro pipe is communicated with the liquid inlet clamping socket, and the other end of the liquid inlet micro pipe is communicated with the detection section;
The liquid inlet microtubes comprise two conductive solution microtubes which are communicated with pipelines on two sides of the flexible conveying belt body through liquid inlet clamping sockets, and bacterial solution microtubes which are arranged between the two conductive solution microtubes and are communicated with a central pipeline in the flexible conveying belt body.
The conductive solution can be effectively guided by the two conductive solution microtubes, and the bacterial solution can be effectively guided by the bacterial solution microtubes; the flow velocity of the two solutions is independently adjusted by the diversion of the two pipelines, so that the flow velocity control is convenient.
Further, the detection section comprises a detection groove, an anode detection port which is arranged at one end of the detection groove and communicated with the conductive solution microtube and the bacterial solution microtube, and a cathode detection port which is arranged at the other end of the detection groove and communicated with the liquid discharge component.
The impedance analyzer can conveniently detect through the arrangement of the anode detection port and the cathode detection port.
Further, two split flow clapboards are arranged inside one end, close to the anode detection port, of the detection groove; the detection groove forms three pre-mixed buffer channels which are respectively communicated with the conductive solution microtube and the bacterial solution microtube through the shunt separator;
one end of the detection groove, which is close to the cathode detection port, is a laminar flow mixing channel which is communicated with the premixing buffer channel and the liquid discharging component.
The laminar flow mixing channel is arranged to enable the high-conductivity solution and the bacterial solution with the same flow rate to form a laminar flow, namely a stable interface is formed on the contact surface of the high-conductivity solution and the bacterial solution, and the high-conductivity solution is prevented from interfering the bacterial solution under the condition of ensuring stable conductivity; ensure stable detection of bacterial solutions.
Further, an anode probe electrically connected with the anode of the impedance analyzer is arranged on the anode detection port;
And a cathode probe electrically connected with the cathode of the impedance analyzer is arranged on the cathode detection port.
The anode probe and the cathode probe can be directly connected with an impedance analyzer to implement accurate electrical impedance detection.
Further, the first negative pressure device and the second negative pressure device comprise negative pressure cavities in the protection box, piston assemblies in the negative pressure cavities, a liquid inlet pump on a liquid inlet of the negative pressure cavities, and one-way valves on the liquid inlet of the negative pressure cavities.
The interference of the liquid inlet pump to the pressure of the negative pressure cavity is cut off through the one-way valve, the problem of pressure instability caused by the liquid inlet pump is avoided, the size of the negative pressure cavity is controlled through the piston component, the liquid pressure is regulated, and the stability of the bacterial solution and the high-conductivity solution is ensured.
Further, a flow rate and flow velocity sensor is arranged at the liquid outlet of the negative pressure cavity.
The arrangement of the flow velocity sensor can accurately detect the bacterial solution and the high-conductivity solution, and is convenient for controlling the flow velocity and the flow velocity of the bacterial solution and the high-conductivity solution respectively.
Further, the power device comprises two groups of linear conveying devices respectively arranged at two sides of the conveying belt;
The linear conveying device comprises a strip-shaped bracket parallel to the conveying belt, a rolling shaft uniformly arranged on the strip-shaped bracket, a conveying chain arranged on the rolling shaft and a servo motor for driving the rolling shaft;
the clamping assembly comprises two clamping conveying belts and clamping plates respectively connected with the conveying chains.
The clamping plates can be driven in a shape through the conveying chain, so that the clamping plates can slide on the conveying belt stably, and the liquid in the conveying belt can be pushed to flow stably through the sliding of the clamping plates.
The detection method adopting the environment-friendly animal bacteria detection device comprises the following steps:
S1, placing the solution
Putting a bacterial solution to be detected into the first liquid supply cavity; placing a high-conductivity solution into the second liquid supply cavity;
s2, matching and adjusting flow velocity
The first negative pressure device of the first-order regulator extracts and conveys the bacterial solution into the middle pipeline of the flexible conveying belt body;
the second negative pressure device extracts and conveys the high-conductivity solution into the other two pipelines of the flexible conveying belt body;
the hydraulic pressure of the two solution supplies is independently regulated by regulating the first negative pressure device and the second negative pressure device; then the clamping assembly is driven by the power device to slide on the flexible conveying belt body, and the flow rates of the two solutions are matched, so that the flow rates of the bacteria solution and the high-conductivity solution are consistent;
s3, microfluidic detection
And introducing the bacterial solution with uniform flow speed and high-conductivity solution into the microfluidic detection channel to detect the impedance of the bacterial solution.
The beneficial effects of the invention are as follows: the invention provides an environment-friendly animal bacteria detection device, which acts on a micro-flow control device for bacteria detection, improves the stability of the flow velocity of liquid in a micro-flow channel, and ensures accurate detection of bacteria; compared with the traditional way of pushing fluid by a pump, the device can ensure the high stability of the liquid pressure in the negative pressure cavity through the first-order regulator, and form a stable constant pressure cavity; the flow rates of the bacteria solution and the high-conductivity solution can be respectively finely adjusted through the arrangement of the first negative pressure device and the second negative pressure device, so that the consistency of the flow rates of the two solutions is realized; the two solutions in the conveying belt are ensured to be stably conveyed by adopting a sliding linear propulsion mode through the second-order regulator, and the flow rates of the two solutions can be buffered and matched; therefore, two solutions form stable laminar flow boundaries in a laminar flow mixing channel, and high mixing of the two solutions is avoided, so that the bacteria detection accuracy is improved.
Drawings
FIG. 1 is a schematic view showing the overall structure of embodiment 1 of the present invention;
FIG. 2 is a schematic diagram showing the structure of a bacteria detection module according to embodiment 1 of the present invention;
FIG. 3 is a schematic view showing the structure of a flow rate regulator according to embodiment 1 of the present invention;
FIG. 4 is a schematic diagram of a second-order regulator according to embodiment 1 of the present invention;
FIG. 5 is a schematic view showing the external structure of a microfluidic detection channel according to embodiment 1 of the present invention;
FIG. 6 is a schematic diagram showing the internal structure of a microfluidic detection channel according to embodiment 1 of the present invention;
Fig. 7 is a schematic diagram of the structures of the first and second negative pressure devices according to embodiment 1 of the present invention;
FIG. 8 is a schematic view showing the structure of a power unit according to embodiment 3 of the present invention;
Wherein, 1-bacteria detection module, 2-solution storage chamber, 20-first liquid storage chamber, 21-second liquid storage chamber, 200-first liquid supply chamber, 201-second liquid supply chamber, 3-impedance analyzer, 4-flow regulator, 40-first main plate, 41-first order regulator, 42-second order regulator, 43-power device, 410-protection box, 411-first negative pressure device, 412-second negative pressure device, 413-negative pressure chamber, 414-liquid inlet pump, 415-check valve, 416-piston assembly, 420-conveyer belt, 421-clamp assembly, 422-flexible conveyer belt body, 423-wear-resistant metal sheet, 430-bar bracket, 431-roller, 432-conveyer chain, 433-servo motor, 5-support frame, 6-multichannel detector, 60-second main plate, 61-micro-flow detection channel, 62-detection section, 63-liquid inlet assembly, 64-liquid discharge assembly, 610-box, 611-liquid inlet card socket, 612-liquid discharge card socket, 621-620-detection groove, 622-detection port, 422-flexible conveyer belt body, 423-wear-resistant metal sheet, 430-bar bracket, 431-roller, 432-conveyer chain, 433-servo motor, 5-support frame, 6-multichannel detector, 60-second main plate, 61-micro-liquid detection channel, 62-micro-flow channel mounting channel, 64-liquid discharge device, 610-micro channel mounting channel, pre-micro channel mounting channel, pre-mixing channel, and/channel 630-mixing channel.
Detailed Description
Example 1
The environment-friendly animal bacteria detection device shown in fig. 1 comprises a bacteria detection module 1, a solution storage cavity 2 communicated with the bacteria detection module 1, and an impedance analyzer 3 electrically connected with the bacteria detection module 1;
the solution storage chamber 2 comprises a first liquid storage chamber 20 for supplying liquid to the bacteria detection module 1 and a second liquid storage chamber 21 for recovering waste liquid;
the first liquid storage chamber 20 comprises a first liquid supply cavity 200 communicated with the bacteria detection module 1 and used for storing a bacteria solution, and a second liquid supply cavity 201 communicated with the bacteria detection module 1 and used for storing a high-conductivity solution;
As shown in fig. 2, the bacteria detection module 1 includes a flow rate regulator 4 in communication with a first liquid supply chamber 200 and a second liquid supply chamber 201, a support frame 5 provided on the flow rate regulator 4, and a multi-channel detector 6 provided on the support frame 5 and in communication with the flow rate regulator 4 and a second liquid storage chamber 21;
The flow rate regulator 4 includes a first main board 40, a first-order regulator 41 uniformly arranged on the first main board 40 and communicated with the first liquid supply cavity 200 and the second liquid supply cavity 201, and a second-order regulator 42 uniformly arranged on the first main board 40 and communicated with the first-order regulator 41 at one end;
As shown in fig. 2, the multi-channel detector 6 includes a second main board 60, and a plurality of micro-flow detection channels 61 uniformly arranged on the second main board 60 and respectively and correspondingly communicated with the other ends of the second-order regulators 42;
As shown in fig. 3, the first-stage regulator 41 includes a protection box 410 disposed on the first main board 40, a first negative pressure device 411 disposed in the protection box 410 and communicating the first liquid supply chamber 200 with the detection channel 61, and a second negative pressure device 412 disposed in the protection box 410 and communicating the second liquid supply chamber 201 with the microfluidic detection channel 61;
As shown in fig. 3 and 4, the second-order regulator 42 includes a conveyor belt 420 disposed on the first main board 40, and having one end in simultaneous communication with the first negative pressure device 411 and the second negative pressure device 412, a clamping assembly 421 movably disposed on the conveyor belt 420, and a power device 43 mounted on the first main board 40 for driving the clamping assembly 421;
The conveyor belt 420 includes a flat flexible conveyor belt body 422, and two wear-resistant metal sheets 423 respectively and correspondingly laid on the surface of the flexible conveyor belt body 422;
Three pipelines are arranged in the flexible conveying belt body 422 in parallel; the central pipeline is used for conveying the bacterial solution, and pipelines on two sides of the central pipeline are used for conveying the high-conductivity solution;
The clamping assembly 421 clamps the wear-resistant metal piece 423 and can slide on the wear-resistant metal piece 423 along the extending direction of the flexible conveying belt body 422.
As shown in fig. 5, the microfluidic detection channel 61 includes a mounting box 610 disposed on the second main board 60, a detection section 62 disposed in the middle of the mounting box 610, and a liquid inlet component 63 and a liquid outlet component 64 disposed in the mounting box 610 and respectively located at the ends of the detection section 62;
one end of the installation box 610 is provided with a liquid inlet clamping socket 611 for communicating the liquid inlet assembly 63 with the conveying belt 420; the other end of the mounting box 610 is provided with a liquid draining clamping socket 612 for communicating the liquid draining assembly 64 with the second liquid storage chamber 21;
As shown in fig. 6, the liquid inlet assembly 63 includes a liquid inlet microtube 630 having one end communicating with the liquid inlet clamping socket 611 and the other end communicating with the detection section 62, and a pipe support 631 for fixing the liquid inlet microtube 630;
The liquid inlet microtube 630 comprises two conductive solution microtubes 632 which are communicated with pipelines on two sides of the flexible conveying belt body 422 through liquid inlet clamping sockets 611, and a bacterial solution microtube 633 which is arranged between the two conductive solution microtubes 632 and is communicated with a central pipeline in the flexible conveying belt body 422.
The detection section 62 includes a detection tank 620, an anode detection port 621 provided at one end of the detection tank 620 and communicating with the conductive solution microtube 632 and the bacterial solution microtube 633, and a cathode detection port 622 provided at the other end of the detection tank 620 and communicating with the liquid discharge unit 64.
Two split flow baffles 623 are arranged inside one end of the detection groove 620 close to the anode detection port 621; the detection groove 620 forms three pre-mixing buffer channels 624 respectively communicated with the conductive solution microtube 632 and the bacterial solution microtube 633 through a shunt separator 623;
The detection tank 620 has a laminar flow mixing channel 625 at one end thereof adjacent to the cathode detection port 622 for communicating the pre-mix buffer channel 624 with the drain assembly 64.
An anode probe 626 electrically connected with the anode of the impedance analyzer 3 is arranged on the anode detection port 621;
the cathode detection port 622 is provided with a cathode probe 627 electrically connected with the cathode of the impedance analyzer 3.
As shown in fig. 7, the first negative pressure device 411 and the second negative pressure device 412 include a negative pressure chamber 413 disposed in the protection box 410, a piston assembly 416 disposed in the negative pressure chamber 413, a liquid inlet pump 414 disposed on a liquid inlet of the negative pressure chamber 413, and a check valve 415 disposed on the liquid inlet of the negative pressure chamber 413.
The liquid outlet of the negative pressure cavity 413 is provided with a flow rate and flow velocity sensor.
The diameters of the inner walls of the conductive solution microtubes 632 and the bacterial solution microtubes 633 were 0.5mm.
The power device 43 is a roller screw, and the clamping assembly 421 is driven by the roller screw.
The roller screw, the flow rate sensor, the check valve 415, the liquid inlet pump 414, the piston assembly 416, and the impedance analyzer 3 all adopt products of the prior art, and specific product types can be selected by those skilled in the art according to requirements.
Example 2
The present embodiment is a detection method using the environment-friendly animal bacteria detection device of embodiment 1, comprising the steps of:
S1, placing the solution
Placing a bacterial solution to be detected into the first liquid supply cavity 200; placing a high conductivity solution into the second liquid supply chamber 201;
s2, matching and adjusting flow velocity
The first negative pressure device 411 of the first-stage regulator 41 extracts and conveys the bacterial solution into the middle pipeline of the flexible conveying belt body 422;
The second negative pressure device 412 draws and delivers the high conductivity solution into the other two conduits of the flexible conveyor body 422;
The hydraulic pressure of the two solution supplies is independently regulated by regulating the first negative pressure device 411 and the second negative pressure device 412; the clamping assembly 421 is driven by the power device 43 to slide on the flexible conveying belt body 422, so that the flow rates of the two solutions are matched, and the flow rates of the bacteria solution and the high-conductivity solution are consistent;
s3, microfluidic detection
The bacterial solution and the high-conductivity solution having the same flow rate are introduced into the microfluidic detection channel 61 to perform impedance detection of the bacterial solution.
Example 3
Unlike example 1, the following is:
As shown in fig. 8, the power device 43 includes two sets of linear conveying devices respectively disposed at two sides of the conveying belt 420;
The linear conveyor includes a bar-shaped bracket 430 parallel to the conveyor belt 420, a roller 431 uniformly disposed on the bar-shaped bracket 430, a conveyor chain 432 disposed on the roller 431, and a servo motor 433 for driving the roller 431;
The clamping assembly 421 includes two clamping plates that clamp the conveyor belt 420 and are connected to the conveyor chains 432, respectively.
Claims (9)
1. The environment-friendly animal bacteria detection device is characterized by comprising a bacteria detection module (1), a solution storage cavity (2) communicated with the bacteria detection module (1), and an impedance analyzer (3) electrically connected with the bacteria detection module (1);
The solution storage cavity (2) comprises a first liquid storage cavity (20) for supplying liquid to the bacteria detection module (1) and a second liquid storage cavity (21) for recycling waste liquid;
The first liquid storage chamber (20) comprises a first liquid supply cavity (200) communicated with the bacteria detection module (1) and used for storing a bacteria solution, and a second liquid supply cavity (201) communicated with the bacteria detection module (1) and used for storing a high-conductivity solution;
The bacteria detection module (1) comprises a flow rate regulator (4) communicated with a first liquid supply cavity (200) and a second liquid supply cavity (201), a support frame (5) arranged on the flow rate regulator (4), and a multichannel detector (6) arranged on the support frame (5) and communicated with the flow rate regulator (4) and a second liquid storage cavity (21);
The flow rate regulator (4) comprises a first main board (40), first-order regulators (41) which are uniformly arranged on the first main board (40) and are communicated with the first liquid supply cavity (200) and the second liquid supply cavity (201), and second-order regulators (42) which are uniformly arranged on the first main board (40) and are communicated with the first-order regulators (41) at one end;
the multichannel detector (6) comprises a second main board (60), and a plurality of micro-flow detection channels (61) which are uniformly arranged on the second main board (60) and are correspondingly communicated with the other ends of the second-order regulators (42) respectively;
The first-order regulator (41) comprises a protection box (410) arranged on the first main board (40), a first negative pressure device (411) arranged in the protection box (410) and communicated with the first liquid supply cavity (200) and the detection channel (61), and a second negative pressure device (412) arranged in the protection box (410) and communicated with the second liquid supply cavity (201) and the micro-flow detection channel (61);
The second-order regulator (42) comprises a conveying belt (420) which is arranged on the first main board (40) and one end of which is simultaneously communicated with the first negative pressure device (411) and the second negative pressure device (412), a clamping assembly (421) which is movably arranged on the conveying belt (420), and a power device (43) which is arranged on the first main board (40) and used for driving the clamping assembly (421);
The conveying belt (420) comprises a flat flexible conveying belt body (422), and two wear-resistant metal sheets (423) are respectively and correspondingly paved on the surface of the flexible conveying belt body (422);
Three pipelines which are arranged side by side are arranged in the flexible conveying belt body (422); the central pipeline is used for conveying the bacterial solution, and pipelines on two sides of the central pipeline are used for conveying the high-conductivity solution;
The clamping assembly (421) clamps the wear-resistant metal sheet (423) and can slide on the wear-resistant metal sheet (423) along the extending direction of the flexible conveying belt body (422).
2. The environment-friendly animal bacteria detection device according to claim 1, wherein the micro-flow detection channel (61) comprises a mounting box (610) arranged on the second main board (60), a detection section (62) arranged in the middle of the mounting box (610), and a liquid inlet component (63) and a liquid outlet component (64) which are arranged in the mounting box (610) and are respectively positioned at the end parts of the detection section (62);
one end of the mounting box (610) is provided with a liquid inlet clamping socket (611) for communicating the liquid inlet assembly (63) with the conveying belt (420); the other end of the mounting box (610) is provided with a liquid draining clamping socket (612) for communicating the liquid draining assembly (64) with the second liquid storage cavity (21);
The liquid inlet assembly (63) comprises a liquid inlet micro tube (630) with one end communicated with the liquid inlet clamping socket (611) and the other end communicated with the detection section (62), and a pipeline support piece (631) for fixing the liquid inlet micro tube (630);
the liquid inlet microtubes (630) comprise two conductive solution microtubes (632) which are communicated with pipelines on two sides of the flexible conveying belt body (422) through liquid inlet clamping sockets (611), and bacterial solution microtubes (633) which are arranged between the two conductive solution microtubes (632) and are communicated with a central pipeline in the flexible conveying belt body (422).
3. The environment-friendly animal bacteria detection device according to claim 2, wherein the detection section (62) comprises a detection groove (620), an anode detection port (621) arranged at one end of the detection groove (620) and communicated with the conductive solution microtube (632) and the bacteria solution microtube (633), and a cathode detection port (622) arranged at the other end of the detection groove (620) and communicated with the liquid discharge assembly (64).
4. The environment-friendly animal bacteria detection device according to claim 2, wherein the detection groove (620) is provided with two split-flow baffles (623) inside at one end close to the anode detection port (621); the detection groove (620) forms three pre-mixing buffer channels (624) which are respectively communicated with the conductive solution microtubes (632) and the bacterial solution microtubes (633) through a shunt separator (623);
One end of the detection groove (620) close to the cathode detection port (622) is provided with a laminar flow mixing channel (625) communicated with the premixing buffer channel (624) and the liquid discharging component (64).
5. The environment-friendly animal bacteria detection device according to claim 2, wherein the anode detection port (621) is provided with an anode probe (626) electrically connected with the anode of the impedance analyzer (3);
and a cathode probe (627) electrically connected with the cathode of the impedance analyzer (3) is arranged on the cathode detection port (622).
6. The environment-friendly animal bacteria detection device according to claim 1, wherein the first negative pressure device (411) and the second negative pressure device (412) comprise a negative pressure cavity (413) arranged in the protection box (410), a piston assembly (416) arranged in the negative pressure cavity (413), a liquid inlet pump (414) arranged on a liquid inlet of the negative pressure cavity (413), and a one-way valve (415) arranged on the liquid inlet of the negative pressure cavity (413).
7. The environment-friendly animal bacteria detection device as claimed in claim 6, wherein the liquid outlet of the negative pressure cavity (413) is provided with a flow rate and flow velocity sensor.
8. An environment-friendly animal bacteria detection device according to claim 1, wherein the power device (43) comprises two sets of linear conveying devices respectively arranged at two sides of the conveying belt (420);
The linear conveyor comprises a bar-shaped bracket (430) parallel to the conveyor belt (420), a roller (431) uniformly arranged on the bar-shaped bracket (430), a conveyor chain (432) arranged on the roller (431), and a servo motor (433) for driving the roller (431);
The clamping assembly (421) comprises two clamping plates which clamp the conveying belt (420) and are respectively connected with the conveying chains (432).
9. The detection method of an environment-friendly animal bacteria detection device according to any one of claims 1 to 8, comprising the steps of:
S1, placing the solution
Putting a bacterial solution to be detected into the first liquid supply cavity (200); placing a high-conductivity solution into the second liquid supply cavity (201);
s2, matching and adjusting flow velocity
The first negative pressure device (411) of the first-stage regulator (41) extracts and conveys the bacterial solution into the middle pipeline of the flexible conveying belt body (422);
The second negative pressure device (412) extracts and conveys the high-conductivity solution into the other two pipelines of the flexible conveying belt body (422);
The hydraulic pressure of the two solution supplies is independently regulated by regulating the first negative pressure device (411) and the second negative pressure device (412); then the clamping component (421) is driven by the power device (43) to slide on the flexible conveying belt body (422) to match the flow rates of the two solutions, so that the flow rates of the bacteria solution and the high-conductivity solution are consistent;
s3, microfluidic detection
And introducing the bacterial solution with uniform flow speed and the high-conductivity solution into a micro-flow detection channel (61) to perform impedance detection of the bacterial solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210897135.7A CN115165975B (en) | 2022-07-28 | 2022-07-28 | Environment-friendly animal bacteria detection device and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210897135.7A CN115165975B (en) | 2022-07-28 | 2022-07-28 | Environment-friendly animal bacteria detection device and detection method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115165975A CN115165975A (en) | 2022-10-11 |
| CN115165975B true CN115165975B (en) | 2024-05-17 |
Family
ID=83478325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210897135.7A Active CN115165975B (en) | 2022-07-28 | 2022-07-28 | Environment-friendly animal bacteria detection device and detection method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115165975B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101694476A (en) * | 2009-09-29 | 2010-04-14 | 北京大学 | Bacteria electric impedance detection method and dedicated chip thereof |
| CN113171807A (en) * | 2021-03-17 | 2021-07-27 | 杭州电子科技大学 | Concentration gradient and bacteria detection integrated micro-fluidic chip and design method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1400195B1 (en) * | 2010-04-15 | 2013-05-24 | Carpigiani Group Ali Spa | BACTERIAL CHARGE CONTROL DEVICE FOR A LIQUID OR SEMILIQUID FOOD PRODUCT. |
| CA2864437C (en) * | 2012-02-15 | 2018-03-13 | Becton, Dickinson And Company | Impedance-based bacterial detection system |
| US9446405B2 (en) * | 2013-03-13 | 2016-09-20 | Joseph Feingold | Microfluidic analyte detection cartridge device, system and method |
-
2022
- 2022-07-28 CN CN202210897135.7A patent/CN115165975B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101694476A (en) * | 2009-09-29 | 2010-04-14 | 北京大学 | Bacteria electric impedance detection method and dedicated chip thereof |
| CN113171807A (en) * | 2021-03-17 | 2021-07-27 | 杭州电子科技大学 | Concentration gradient and bacteria detection integrated micro-fluidic chip and design method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 应用电化学测菌法检测牙龈卟啉单胞菌的初步研究;裴振华;朱涛;施生根;温颖;汤楚华;熊春阳;;牙体牙髓牙周病学杂志;20110515(第05期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115165975A (en) | 2022-10-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2021010379A (en) | Actuation systems and methods for use with flow cells. | |
| CN115165975B (en) | Environment-friendly animal bacteria detection device and detection method thereof | |
| US20230107476A1 (en) | Structures for automated, multi-stage processing of nanofluidic chips | |
| DK1902295T3 (en) | Homogeneous mixing and distribution of a reactant on a surface | |
| CN101694476A (en) | Bacteria electric impedance detection method and dedicated chip thereof | |
| CN104004655A (en) | Microbial electrochemical system for continuous culture, sampling and real-time detection | |
| KR101491823B1 (en) | Microfluidic floating block and an combining method of microfluidic floating block by using these. | |
| CN111304060A (en) | Micro-droplet generation chip | |
| CN104764860B (en) | A kind of analysis metering device and liquid analysis system | |
| CN221117405U (en) | Chip sequencing system for rapid sample injection | |
| CN213266464U (en) | Continuous liquid adding device for full-automatic nucleic acid extraction instrument | |
| CN104730216A (en) | Analyzing and measuring device and liquid analyzing system | |
| CN114518240A (en) | Online sample analysis device | |
| CN115386472A (en) | Micro-fluidic card box of nucleic acid detection card | |
| US20220065890A1 (en) | Device, System and Method for Fluid Delivery for Sequencing | |
| CN211715280U (en) | Gas pressurizing and evacuating dual-purpose pump for micro-fluidic tester | |
| CN109971630B (en) | Nanopore chip clamp | |
| CN212640508U (en) | Integrated PCR instrument | |
| CN212385383U (en) | Filter element assembling machine | |
| CN204575616U (en) | A kind of analysis measuring apparatus and liquid analysis system | |
| CN104764861B (en) | Analyzing and metering device and liquid analysis system | |
| CN102719355A (en) | Cell vacuum absorption fixing device | |
| US9731295B2 (en) | Fluid connection device for biological analysis apparatuses, suitable fluidic component and biological analysis device equipped with same | |
| CN216816707U (en) | Device for spotting multiple biological reagents | |
| CN115266896B (en) | Biological sample analysis system and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |