CN114983874A - Traditional Chinese medicine volatile oil composition mouthwash - Google Patents
Traditional Chinese medicine volatile oil composition mouthwash Download PDFInfo
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- CN114983874A CN114983874A CN202210738409.8A CN202210738409A CN114983874A CN 114983874 A CN114983874 A CN 114983874A CN 202210738409 A CN202210738409 A CN 202210738409A CN 114983874 A CN114983874 A CN 114983874A
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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Abstract
The invention discloses a preparation method of a traditional Chinese medicine essential oil mouthwash mainly having the functions of inhibiting oral bacteria and treating periodontitis, and belongs to the technical field of oral health. The traditional Chinese medicine bacteriostatic essential oil mouthwash disclosed by the invention is prepared from the following raw materials: litsea cubeba (Latin name: Litsea cubeba) essential oil, folium artemisiae argyi (Latin name: Artemisia princeos) essential oil and hairyvein Agrimonia herb and bud (Latin name: Agrimonia pilosa Ledeb) essential oil. The traditional Chinese medicine bacteriostatic essential oil mouthwash disclosed by the invention is reasonable in compatibility, and the components act synergistically, so that the traditional Chinese medicine bacteriostatic essential oil mouthwash can effectively inhibit bacteria and clean oral cavities, and has the effects of inhibiting gingival bleeding, eliminating dampness and reducing phlegm. In addition, the invention has simple preparation process and wide applicable population, can be used by pregnant women and children, and has no adverse reaction.
Description
Technical Field
The invention discloses a composition of a traditional Chinese medicine essential oil composition gargle with the main function of inhibiting oral bacteria and a preparation method thereof, belonging to the technical field of oral health.
Background
In china, oral health is receiving unprecedented attention. With the development of economy and the improvement of living standard, the consciousness of people on oral health care is also greatly enhanced. With the promotion and change of market demand and consumption concept, the oral care product format has gradually upgraded from the original toothpaste "all the year round" to the joint operation of toothpaste, dental floss, tooth paste, gargle, electric toothbrush and other products. Generally speaking, the conventional tooth brushing can only remove 40-50% of dental plaque on the tooth surface, and can only remove 30% of dental plaque on the adjacent surface, but has no effect on removing the dental plaque on the soft tissue surface of the oral cavity. The pathogenic bacteria porphyromonas gingivalis in the plaque cause damage to periodontal tissues by releasing toxic products, eventually leading to periodontitis. However, the gargle can go deep into various parts of the oral cavity, including the gingival margin and the gaps between teeth which are difficult to be contacted by the toothbrush, and is a good measure for assisting in controlling dental plaque.
Staphylococcus aureus and porphyromonas gingivalis are among the causes affecting oral health. Staphylococcus aureus is a gram-positive bacterium, Porphyromonas gingivalis is a gram-negative bacterium.
The traditional Chinese medicine theory considers that the stomach fire is hot, the kidney qi is deficient and the qi and blood are insufficient, which are main causes of gum problems. Gargle liquid plays an important role in the prevention and adjuvant therapy of oral diseases. At present, the gargle which is commonly used clinically mostly contains antibiotics, iodine, ethanol and other components, such as chlorhexidine, novel iodine-containing gargle and the like, and the components can effectively reduce bacteria in dental plaque, weaken the toxicity of bacterial products, achieve the purposes of controlling the dental plaque and preventing oral diseases such as gingivitis, periodontitis and the like. However, the oral flora is easily disordered after the gargle containing broad-spectrum antibiotics is used for a long time, and the risks of drug-resistant bacteria and secondary infection are higher along with the increase of the use times. The chlorhexidine gargle is easy to cause tooth surface coloring and taste change after long-term use, is not suitable for long-term use, is not accepted by patients due to bitter taste, poor taste and large local stimulation, and even generates allergy to some patients to cause rash or stomatitis. The iodine-containing gargle has unstable effective iodine content, and the effective content is reduced quickly after dilution, so that the gargle needs to be prepared as it is used, which brings inconvenience to patients. Therefore, finding a natural, effective, safe, alcohol-free, long-term use mouthwash with few side effects is a new hotspot for treating oral problems.
The natural medicines have long application history, small toxic and side effects, convenient material taking, economy and practicability, and also have the functions of sterilization and inflammation diminishing, so the natural medicines are widely used in basic research and clinical practice. The Chinese medicinal preparation is widely applied to the oral cavity, and a large number of researches show that the Chinese medicament has good curative effect on treating diseases such as periodontal diseases, oral ulcer and the like. However, most of the reports of the traditional Chinese medicines at present are the effects of a single Chinese herbal medicine, the research on the traditional Chinese medicine compound is less, and the traditional Chinese medicine compound can reflect the characteristics of treatment based on syndrome differentiation of the traditional Chinese medicine, and fully play the synergistic effect of the traditional Chinese medicine and the multi-target effect of various active ingredients of the traditional Chinese medicine.
Mugwort (Latin name: Artemisia princeos) essential oil: the folium artemisiae argyi essential oil has extremely strong molecule permeability, is easy to be quickly permeated and absorbed by the skin, further regulates qi and blood, relieves cough, reduces phlegm, participates in metabolism and improves immunity. Folium Artemisiae Argyi is pungent, bitter and warm, and has effects of stopping bleeding, relieving pain, and removing deficient fire and cold fire. The diseases of oral ulcer, stomatitis, sore throat and the like in human bodies are all caused by the exuberance of deficient fire or cold fire in the bodies, and the argyi leaf essential oil has good relieving effect on the diseases.
Agrimonia pilosa (Latin name: Agrimonia pilosa Ledeb) essential oil: the agrimony essential oil has the effects of astringing to stop bleeding, stopping dysentery, killing parasites and the like. Can be used for treating oral cavity problems such as gingival hemorrhage and oral cavity bitter.
Litsea cubeba (Latin name: Litsea cubeba) essential oil: the litsea cubeba essential oil has good antibacterial action on most microorganisms, has better effect on gram-positive bacteria, and can promote cell regeneration, repair skin cell tissues and relieve skin problems of inflammation and swelling.
Disclosure of Invention
The invention aims to provide a formula and a preparation method of a traditional Chinese medicine bacteriostatic essential oil combined mouthwash. The traditional Chinese medicine bacteriostatic essential oil mouthwash disclosed by the invention is reasonable in compatibility, and the components act synergistically, so that the traditional Chinese medicine bacteriostatic essential oil mouthwash can effectively inhibit bacteria and clean oral cavities, and has the effects of inhibiting gingival bleeding, eliminating dampness and reducing phlegm. In addition, the invention has simple preparation process and wide applicable population, can be used by pregnant women and children, and has no adverse reaction.
The technical scheme provided by the invention is as follows:
a traditional Chinese medicine essential oil collutory for inhibiting oral bacteria is prepared from fructus Litseae essential oil, folium Artemisiae Argyi essential oil and herba et Gemma Agrimoniae essential oil at a mass ratio of 1-2: 1-2.
Preferably, the mass ratio of the litsea cubeba essential oil, the argy wormwood leaf essential oil and the hairyvein agrimony essential oil is 1: 1.
The invention also provides a preparation method of the traditional Chinese medicine essential oil mouthwash for inhibiting oral bacteria, which comprises the following steps:
mixing fructus Litseae essential oil, folium Artemisiae Argyi essential oil and herba et Gemma Agrimoniae essential oil uniformly to obtain the Chinese medicinal essential oil collutory for inhibiting oral bacteria.
The invention also protects the application of the traditional Chinese medicine compound essential oil in inhibiting oral bacteria and treating periodontitis, and the application in keeping clean oral cavity, inhibiting gingival bleeding, eliminating dampness and reducing phlegm.
Compared with the prior art, the invention has the beneficial effects that:
the technical scheme of the invention adopts the natural Chinese medicinal bacteriostatic essential oil mouth wash which has reasonable compatibility and synergistic effect among components, and can obviously reduce the number of oral microorganisms including porphyromonas gingivalis, staphylococcus aureus and the like, thereby reducing the morbidity of periodontitis caused by the porphyromonas gingivalis, and oral ulcer and angular stomatitis caused by the staphylococcus aureus, escherichia coli, candida albicans and the like. The invention has the effects of cleaning oral cavity, inhibiting gingival bleeding, eliminating dampness and phlegm. In addition, the traditional Chinese medicine compound essential oil disclosed by the invention is simple in preparation process, beneficial to realization of industrialization, wide in applicable group, applicable to both pregnant women and children, and free of adverse reaction.
Drawings
FIG. 1 is a comparison graph of the bacteriostatic ring diameters of the equivalent amounts of the Chinese medicinal bacteriostatic essential oil mouth rinse (0.5%), metronidazole gargle (0.5%) and distilled water control sample prepared in examples 1-7 and comparative examples 1-6 of the invention, which are measured against Staphylococcus aureus. Unless otherwise specified, the above sample specimen contains water in the amounts other than the labeled components.
FIG. 2 is a comparison graph of the diameters of bacteriostatic rings of the same amounts of the Chinese medicinal bacteriostatic essential oil mouth rinse (0.5%), the metronidazole gargle (0.5%) and the distilled water control sample prepared in examples 1-7 and comparative examples 1-6 of the present invention measured against Porphyromonas gingivalis.
Unless otherwise indicated, the sample specimens of FIGS. 1 and 2 contain water in amounts other than the indicated ingredients.
Detailed Description
The K-B paper diffusion in vitro bacteriostasis test in the examples and comparative examples (i.e. attaching paper containing quantitative drug to specific area of agar plate inoculated with bacteria to be tested, the drug inhibiting bacteria growth to form transparent bacteriostasis ring to reflect sensitivity of bacteria to be tested to drug) is as follows:
(1) and uniformly coating 120 mu L of staphylococcus aureus liquid with the concentration of 1000 times diluted on a prepared agar plate, and standing until the liquid is absorbed.
(2) Placing 10 pieces of filter paper sheets in a glass plate; shaking the sample vigorously, quickly sampling 10 μ L, dripping into filter paper, sticking filter paper sheet onto the surface of agar coated with bacterial liquid with aseptic forceps, lightly pressing the paper sheet to make it contact with agar, sticking three paper sheets on each plate, and paralleling the three plates to make the paper sheets have equal distance. And then culturing in an incubator at 37 ℃ for 24h, observing the inhibition zone, measuring the diameter of each inhibition zone by using a vernier caliper, and counting by taking the average value.
The bacteriostatic tests on Porphyromonas gingivalis in the examples and comparative examples were as follows: the anaerobic blood agar culture medium is operated on a clean workbench with an anaerobic incubator to prepare a culture dish. 50. mu.L of a bacterium solution of Porphyromonas gingivalis was taken out from the culture medium, applied with a sterile coating bar, allowed to dry naturally, and then punched out in the culture medium with a sterilized puncher, and 15. mu.L of each of the solutions prepared in examples and comparative examples was dropped into the holes. And (3) placing the culture medium in an anaerobic culture bag, placing the culture medium in an incubator at 37 ℃ for culturing for 16h, observing the diameter of the inhibition zone, and recording. Each group of drugs was repeated 3 times in parallel and the average was taken.
The use method of the Chinese medicinal bacteriostatic essential oil gargle in each example and comparative example comprises the following steps: taking 0.1g of the Chinese medicinal antibacterial essential oil gargle, adding clear water to dilute to 20ml, shaking up violently, holding for 3min first, and then gargling.
The application method of the metronidazole gargle (0.5% of the specification of the lotus and metronidazole gargle in cloud): gargling, 20ml for one time, first for 3min and then gargling.
Weighing the litsea cubeba essential oil, the argy wormwood leaf essential oil and the hairyvein agrimony essential oil according to the weight ratio, and then uniformly mixing to obtain the traditional Chinese medicine essential oil mouthwash for inhibiting oral bacteria. The examples and comparative formulations are shown in table 1:
TABLE 1 examples, comparative example formulations (parts by weight)
| Litsea cubeba essential oil/part | Folium Artemisiae Argyi essential oil/part | Agrimonia pilosa L essential oil/part | |
| Example 1 | 12 | 6 | 6 |
| Example 2 | 6 | 6 | 12 |
| Example 3 | 6 | 12 | 6 |
| Example 4 | 9 | 9 | 6 |
| Example 5 | 9 | 6 | 9 |
| Example 6 | 6 | 9 | 9 |
| Example 7 | 8 | 8 | 8 |
| Comparative example 1 | 12 | 12 | 0 |
| Comparative example 2 | 0 | 12 | 12 |
| Comparative example 3 | 12 | 0 | 12 |
| Comparative example 4 | 24 | 0 | 0 |
| Comparative example 5 | 0 | 24 | 0 |
| Comparative example 6 | 0 | 0 | 24 |
Performance testing of Chinese medicinal bacteriostatic essential oil mouthwash
(I) mouthfeel and odor testing
60 patients with oral problems such as oral ulcer and gingival bleeding are selected, and the average age of the patients is 35 years old. The inventive examples 1-7 were used as the test group, and the nyuqin metronidazole gargle was used as the control group. The results are shown in table 2:
table 2 taste and odor test results
| Group of | Colour(s) | Taste of the product | Smell(s) |
| Example 1 | Light yellow | Refreshing and non-irritant | Fragrance |
| Example 2 | Light yellow | Refreshing and non-irritant | Fragrance |
| Example 3 | Light yellow | Refreshing and non-irritant | Fragrance |
| Example 4 | Light yellow | Refreshing and non-irritant | Faint scent |
| Example 5 | Light yellow | Refreshing and non-irritant | Fragrance |
| Example 6 | Light yellow | Refreshing and non-irritant | Fragrance |
| Example 7 | Light yellow | Refreshing and non-irritant | Fragrance |
| Metronidazole gargle | Colorless or slightly yellow | Has strong irritation | Has bitter taste |
Table 2 shows that the mouth feel and smell of the mouth rinse of examples 1-7 (the mouth rinse of bacteriostatic traditional Chinese medicine essential oil prepared by uniformly mixing the litsea cubeba essential oil, the argyi leaf essential oil and the agrimony essential oil) and the metronidazole mouth rinse are tested, and the results show that the mouth rinse of examples 1-7 of the invention is fresh and non-irritant, pleasant in smell and strong in compliance, while the mouth rinse of metronidazole is bitter in taste, is difficult for patients to accept, and causes hindrance to treatment.
(II) Long-term use Effect test
300 patients with oral problems such as oral ulcer and gingival bleeding are selected, and the average age of the patients is 35 years old. Groups were randomized into 15 groups of 20 people each.
The patient gargles with the same amount (20mI) of the antibacterial essential oil gargle, the metronidazole gargle and the distilled water prepared in examples 1-7 and comparative examples 1-6, and then gargles for 3 min. Gargling three times a day after meals in the morning, at noon and evening for 2 weeks.
The curative effect standard is as follows: obviously improve the following steps: oral problems such as oral ulcer, gingival bleeding, and oral odor are obviously improved or disappeared. The improvement is as follows: oral problems were improved to some extent but not significantly. And (4) invalidation: oral problems were not improved. Deterioration: the oral problems are further exacerbated.
The results are shown in Table 3:
table 3 long-term use effect test results
Table 3 shows the effect test of rinsing three times after breakfast, noon and supper for continuous use for 2 weeks in the morning of inventive examples 1-7 and comparative examples 1-6. Comparing examples 1 to 7, the results show that the use effect of example 3 is the best, i.e., the oral cavity problem is improved to the greatest extent when the mass ratio of the litsea cubeba essential oil, the artemisia argyi essential oil and the agrimonia pilosa essential oil is 1: 1. Comparing example 7 with comparative examples 1-6, the ranking of the use effect is (from excellent to inferior) example 7 > comparative example 1 > comparative example 3 > comparative example 2 > comparative example 6 > comparative example 5 > comparative example 4, the ranking of the use effect shows that the traditional Chinese medicine bacteriostatic essential oil mouthwash of the invention has reasonable compatibility, the agrimony essential oil, the argy wormwood leaf essential oil and the litsea cubeba essential oil can generate synergistic effect, the effect is better than that of singly using one kind of essential oil or singly using any two kinds of essential oils, and the curative effect is more obvious. In addition, as can be seen from table 2, the use effects of examples 1 to 7 and comparative examples 1 to 6 are better than that of metronidazole gargle.
FIG. 1 is a comparison graph of the diameter of bacteriostatic rings of the traditional Chinese medicine bacteriostatic essential oil gargle (0.5%), metronidazole gargle (0.5%) and distilled water control samples prepared according to the same amount of the traditional Chinese medicine bacteriostatic essential oil gargle (0.5%) and the comparative examples 1-6, which are measured against staphylococcus aureus. As can be seen from fig. 1, the bacteriostatic effect: example 7 > example 3 > example 2 > example 4 > example 1 > example 6 > example 5 > comparative example 2 > metronidazole mouthrinse > comparative example 1 > comparative example 3 > comparative example 4 > comparative example 5 > comparative example 6 > distilled water, demonstrating that the bacteriostatic effect on staphylococcus aureus is most pronounced when the mass ratio of litsea cubeba essential oil, argyi leaf essential oil and agrimonia essential oil is 1: 1, and litsea cubeba essential oil, argyi leaf essential oil and agrimonia essential oil act synergistically, better than that of either one or any two of them alone.
FIG. 2 is a comparison graph of the diameters of bacteriostatic rings of the same amounts of the Chinese medicinal bacteriostatic essential oil mouth rinse (0.5%), the metronidazole gargle (0.5%) and the distilled water control sample prepared in examples 1-7 and comparative examples 1-6 of the invention, which are measured against Porphyromonas gingivalis. As can be seen from fig. 2, the bacteriostatic effect: example 7 > example 5 > example 6 > example 2 > example 1 > example 3 > metronidazole mouthrinse > comparative example 1 > comparative example 3 > comparative example 4 > comparative example 2 > comparative example 5 > comparative example 6 > distilled water, demonstrating that the bacteriostatic effect on porphyromonas gingivalis is most pronounced when the mass ratio of litsea cubeba essential oil, argy wormwood leaf essential oil and agrimony essential oil is 1: 1, and litsea cubeba essential oil, argy wormwood leaf essential oil and agrimony essential oil act synergistically, better than that of staphylococcus aureus when either one or any two of the essential oils are used alone.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (4)
1. A traditional Chinese medicine essential oil mouthwash for inhibiting oral bacteria is characterized in that the mass ratio of Litsea cubeba essential oil, argyi leaf essential oil and hairyvein Agrimonia herb essential oil is 1-2: 1-2, and the traditional Chinese medicine essential oil mouthwash is applied to inhibiting oral bacteria, treating periodontitis, keeping the oral cavity clean, inhibiting gingival bleeding, eliminating dampness and reducing phlegm.
2. The traditional Chinese medicine essential oil mouth wash for inhibiting oral bacteria according to claim 1, wherein the mass ratio of the litsea cubeba essential oil, the argyi leaf essential oil and the hairyvein agrimonia herb essential oil is 1: 1.
3. The preparation method of the traditional Chinese medicine essential oil mouth wash for inhibiting oral bacteria according to any one of claim 1, which comprises the following steps:
mixing fructus Litseae essential oil, folium Artemisiae Argyi essential oil and herba et Gemma Agrimoniae essential oil uniformly to obtain the Chinese medicinal essential oil collutory for inhibiting oral bacteria.
4. The application of the traditional Chinese medicine compound essential oil in inhibiting oral bacteria and treating periodontitis, and the application in keeping clean oral cavity, inhibiting gingival bleeding, eliminating dampness and phlegm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102397176A (en) * | 2010-09-17 | 2012-04-04 | 南京星银药业集团有限公司 | A Chinese medicinal collutory and its preparation method |
| CN103536497A (en) * | 2013-10-30 | 2014-01-29 | 昆明理工大学 | Traditional Chinese medicinal compound collutory and preparation method thereof |
| WO2017206015A1 (en) * | 2016-05-30 | 2017-12-07 | 江苏奇力康皮肤药业有限公司 | Long-lasting antibacterial and tooth protection gargle |
| CN113081900A (en) * | 2021-04-06 | 2021-07-09 | 河北科技大学 | Gargle and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102397176A (en) * | 2010-09-17 | 2012-04-04 | 南京星银药业集团有限公司 | A Chinese medicinal collutory and its preparation method |
| CN103536497A (en) * | 2013-10-30 | 2014-01-29 | 昆明理工大学 | Traditional Chinese medicinal compound collutory and preparation method thereof |
| WO2017206015A1 (en) * | 2016-05-30 | 2017-12-07 | 江苏奇力康皮肤药业有限公司 | Long-lasting antibacterial and tooth protection gargle |
| CN113081900A (en) * | 2021-04-06 | 2021-07-09 | 河北科技大学 | Gargle and preparation method and application thereof |
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