CN114259276B - High-activity bone construction method based on bone marrow aspiration enrichment system - Google Patents
High-activity bone construction method based on bone marrow aspiration enrichment system Download PDFInfo
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Abstract
The invention belongs to the technical field of bone tissue engineering, and particularly relates to a high-activity bone construction method based on a bone marrow aspiration enrichment system, which comprises the following steps: placing the allogeneic bone decalcification stent in a prepared peptide solution for soaking, and flushing after the soaking is finished to obtain the allogeneic bone decalcification stent modified by self-assembled polypeptide; placing the prepared enrichment material into an inner tank body of a mixing stirring tank of a bone marrow aspiration enrichment system, and enriching according to preset time; and (5) discharging the waste liquid in the mixing stirring tank after the enrichment is finished, so as to obtain the constructed high-activity bone repair material. The invention can solve the problems of inaccurate bone taking mode and easy loss of bone forming materials in the prior art.
Description
Technical Field
The invention belongs to the technical field of bone tissue engineering, and particularly relates to a high-activity bone construction method based on a bone marrow aspiration enrichment system.
Background
At present, with the rapid development of the medical industry, the technology in various fields of medical treatment is rapidly developed, and various novel medical technologies are developed, but partial defects exist, wherein the technology related to bone injury and repair of bone defect is a great difficulty in orthopedics. The current method for solving the bone injury and bone defect is a bone grafting method, and the method for solving the defect of the bone quantity of the implantation site in the bone grafting method is to utilize autologous bone, allogeneic bone, xenogeneic bone and artificial bone for grafting; autologous bone grafting is a gold standard of bone grafting methods due to good tissue compatibility, bone conductivity, bone inducibility and no immunogenicity, and has wide clinical application. However, because the autologous bone source is limited, the requirement of a large number of bone transplants is difficult to meet, pain of the bone taking part and appearance influence can be caused, or serious complications such as blood vessels, nerve injury, deep infectious hematoma, ilium wing fracture and the like are caused, so that proper autologous bone material taking sites and material taking methods are selected, the trauma of an autologous bone transplant supply area is effectively reduced, and the method is an important point of the current autologous bone transplant research.
The traditional bone taking mode is to use bone taking mechanisms such as an osteotome, a bone scoop and the like to be matched with repeated physical knocking and separating bone taking, the bone taking is inaccurate and peripheral vascular nerves are easily damaged, in recent years, along with the rapid development of regenerative medicine, stem cell treatment provides a new approach for solving the current difficult problem of refractory large bone defects and the future development of orthopedics. At present, the conventional bone marrow mesenchymal stem cells (BMSCs) are obtained by puncturing and extracting the upper iliac spines, the sternum and the rib, but due to the limitation of a bone taking method and quality, the bone taking method is inaccurate, and meanwhile, the stem cells are required to be cultured after the bone taking, so that the stem cells are easy to run off.
Disclosure of Invention
The invention aims to provide a high-activity bone construction method based on a bone marrow aspiration enrichment system, which aims to solve the problems that the bone extraction mode is inaccurate and bone materials are easy to lose in the prior art.
The basic scheme provided by the invention is as follows: the utility model provides a marrow aspiration enrichment system, includes expands marrow drill bit, drilling rod, collecting pipe and mixes stirring jar, it is connected to expand marrow drill bit and drilling rod can dismantle, it is hollow to expand marrow drill bit and drilling rod, it is a drainage hole that eccentric negative angle set up to expand the marrow drill bit be equipped with a plurality of, the drilling rod rear end is equipped with actuating mechanism, actuating mechanism and drilling rod sliding connection, the collecting pipe parcel drilling rod, the collecting pipe rear end is equipped with water inlet and delivery port, water inlet and drilling rod afterbody intercommunication, mix stirring jar includes outer jar body and inner tank body, be equipped with negative pressure suction device between outer jar body and the inner tank body, contain enrichment material and mix stirring device in the inner tank body, the delivery port passes through pipeline and outer jar body intercommunication, inner tank body bottom is equipped with vortex device.
The basic scheme one provided by the invention has the principle and advantages that: in the prior art, bone taking devices such as an osteotome, a bone scoop and the like are matched with repeated physical knocking to separate and take bones, the bone taking is inaccurate, peripheral vascular nerves are easily damaged, and a large amount of autologous bone materials are lost in the bone taking process.
Therefore, the invention aims at the problems existing in the prior art, the driving mechanism is in sliding connection with the drill rod, before the operation, the driving mechanism and the drill rod are in a non-contact state, the normal saline is injected into the drill rod through the water inlet, when the operation is performed, the drill rod is propped against the driving mechanism, the drill rod is controlled to further control the marrow expanding drill bit to obtain the required bone raw material mixed solution by means of negative pressure and store the required bone raw material mixed solution into the collecting pipe, then the bone raw material mixed solution flows into the outer tank body of the mixing stirring tank through the water outlet of the collecting pipe, and is pumped into the inner tank body through the negative pressure pumping device, the bone raw material mixed solution is absorbed by the enrichment material in the inner tank body, and is stirred by the mixing stirring device, so that the enrichment material moves up and down, the bone material is fully enriched, and simultaneously the turbulent flow device enables the bone raw material deposited at the bottom of the inner tank body to flow, so that the enrichment material can be enriched with more bone material. Therefore, the invention has the advantages that: (1) The hollow drill bit is used, bone can be taken from the diaphysis intramedullary cavity under the guidance of the guide pin, and the marrow expanding drill bit and the drill rod are driven by the driving mechanism, so that negative pressure suction generated by high-speed rotation is generated in the process of obtaining bone raw materials, and the problem of inaccurate bone taking cannot be caused by a fixed-point drilling mode; (2) The mixed liquid flowing into the outer tank is sucked into the inner tank by negative pressure, the enrichment material of the inner tank body can absorb the autologous bone material in the mixed liquid, meanwhile, the negative pressure suction device sucks the liquid to enter through the rotation of the inner tank body, the mixing stirring device is pushed to enable the enrichment material to be mixed in a multidirectional mode under the condition of normal collection, the autologous bone material is fully enriched, the turbulence device can enable the autologous bone material precipitated at the bottom of the inner tank to flow in the inner tank, and therefore the enrichment material can enrich more autologous bone material.
Further, the enrichment material is an allogeneic bone decalcification stent, and the surface of the allogeneic bone decalcification stent is modified by self-assembled polypeptide.
The beneficial effects are that: the high-efficiency enrichment material based on the allogeneic bone utilizes the mild self-assembly characteristic of the self-assembly polypeptide to carry out surface modification on the allogeneic bone, thereby improving the enrichment capacity and the adhesion characteristic of the enrichment material.
Further, the mixing stirring device comprises a stirring shaft and stirring blades, the stirring blades are positioned on the stirring shaft, one end of the stirring shaft is connected with the turbulence device, and the other end of the stirring shaft is connected with the external driving device.
The beneficial effects are that: the stirring effect can be realized through the stirring blade, and the stirring shaft is connected with the turbulence device, so that the turbulence device is driven to rotate when the stirring shaft rotates, and then the power device is saved.
Further, the turbulence device is a propeller blade.
The beneficial effects are that: turbulence can be achieved by the propeller blades.
Further, be equipped with double helix structure on the drilling rod, the collecting tube joint of drilling rod double helix department has protection device.
The beneficial effects are that: the drill rod part is arranged to be of a double-screw structure, local negative pressure is formed through high-speed rotation, unidirectional guiding-out of an osteogenic material is facilitated, meanwhile, a protection device which is connected with a collecting pipe in a clamping mode is arranged at the double-screw position, the collecting pipe can be prevented from being scratched by the double-screw structure, and stability is guaranteed.
Further, still include the waste liquid collection tank, mix and stir jar internal tank bottom and be equipped with liquid outlet and filter equipment, the waste liquid collection tank passes through the pipe connection liquid outlet.
The beneficial effects are that: can filter out the waste liquid through filter equipment and waste liquid collection jar on the one hand, on the other hand waste liquid collection jar can collect medical waste, avoids causing medical pollution.
Further, the reamer bit is a replaceable reamer bit, and the reamer bit is 10-13 mm in specification.
The beneficial effects are that: the reaming bit is arranged to be replaceable and can be suitable for reaming of different parts within different specification ranges.
Further, the driving mechanism is a speed regulating motor, and the drill rod is positioned on a rotating shaft of the speed regulating motor.
The beneficial effects are that: the rotating speed of the reamer head can be adjusted by the speed regulating motor according to actual conditions, and the applicability of the reamer system is improved.
The basic scheme II provided by the invention is as follows: a high-activity bone construction method based on a bone marrow aspiration enrichment system is applied to the bone marrow aspiration enrichment system, and comprises the following steps:
Preparing an enrichment material: placing the allogeneic bone decalcification stent in a prepared peptide solution for soaking, and flushing after the soaking is finished to obtain the allogeneic bone decalcification stent modified by self-assembled polypeptide;
Enrichment: placing the prepared enrichment material into an inner tank body of a mixing stirring tank of a bone marrow aspiration enrichment system, and enriching according to preset time;
The step of constructing high-activity bone: and (5) discharging the waste liquid in the mixing stirring tank after the enrichment is finished, so as to obtain the constructed high-activity bone repair material.
The principle and effect of the basic scheme II provided by the invention are as follows: in the invention, firstly, the allogeneic bone decalcification stent modified by self-assembled polypeptide is prepared, and the allogeneic bone decalcification stent modified by self-assembled polypeptide is placed in a mixing stirring tank of a bone marrow aspiration enrichment system to enrich the required bone-forming material, after a period of time, enrichment is completed, and the waste liquid in the mixing stirring tank is discharged to obtain the constructed high-activity bone repair material. Therefore, the second basic scheme of the invention has the advantages that compared with the stem cell acquisition mode in the prior art, the enrichment can be performed immediately in the stem cell acquisition process, the problem of massive loss of stem cells is avoided, and meanwhile, the acquired high-activity bone repair material is sufficient, so that the frequent bone acquisition step is reduced.
Further, the step of preparing the enriched material further comprises the following steps:
The step of constructing an osteoinductive membrane: filling the bone defect part with bone cement to form an osteoinductive membrane;
The step of constructing the highly active bone further comprises the following steps:
and (3) repairing: taking out the bone cement, and implanting the constructed high-activity bone repair material into the induction membrane of the bone defect part.
The beneficial effects are that: by filling bone cement in the bone defect, the bone induction membrane with good bone formation activity can be cultivated, and the constructed high-activity bone is implanted into the bone defect, so that the bone defect can be well repaired.
Drawings
FIG. 1 is a partial cutaway view of an expanded portion of an embodiment of the present invention;
FIG. 2 is an overall view of an embodiment of the present invention;
FIG. 3 is a cross-sectional view of an embodiment of the reamer head of the present invention;
FIG. 4 is a cross-sectional view of a tail portion of a collection tube according to an embodiment of the present invention;
FIG. 5 is an overall view of a hybrid agitation tank in accordance with an embodiment of the present invention;
FIG. 6 is a top overall view of a hybrid agitation tank according to an embodiment of the present invention;
Fig. 7 is a flow chart of an embodiment of the present invention.
Detailed Description
The following is a further detailed description of the embodiments:
the labels in the drawings of this specification include: the device comprises a reaming bit 1, a drainage hole 101, a drill pipe 2, a drill pipe seal 201, a collecting pipe 3, a water outlet 301, a water inlet 302, a mixing and stirring tank 4, an inner tank 401, an outer tank 402, a mixing and stirring device 403, a turbulence device 404, a waste liquid collecting tank 5, a speed regulating motor 6, a locking clamp cover 7 and a sleeve pipe 8.
The embodiment is substantially as shown in figures 1,2 and 3: the marrow aspiration enrichment system comprises a marrow expanding drill bit 1, a drill rod 2, a collecting pipe 3, a mixing stirring tank 4 and a waste liquid collecting tank 5, wherein the marrow expanding drill bit 1 and the drill rod 2 are detachably connected in a threaded connection mode, the marrow expanding drill bit 1 and the drill rod 2 are hollow, the marrow expanding drill bit 1 is conical in the embodiment, a plurality of diversion holes 101 which are arranged in an eccentric negative angle are formed in the marrow expanding drill bit 1, in the embodiment, the marrow expanding drill bit 1 and the drill rod 2 are made of special materials of stainless steel, the hardness and the anti-rotation toughness of the marrow expanding drill bit 1 can be ensured, 4 diversion holes 101 are arranged, the eccentric negative angle of the diversion holes 101 is specifically-20 DEG, and the physiological saline can flow smoothly under the high-speed rotation condition can be ensured, so that blockage is prevented and diversion can be carried out; meanwhile, the reamer bit 1 is a replaceable reamer bit, the specification range of the reamer bit 1 is between 10mm and 13mm, in the embodiment, one specification is set for each 0.5mm of the reamer bit 1, namely, the reamer bit 1 with the specifications of 10mm, 10.5 mm, 11 mm, 11.5 mm, 12 mm, 12.5 mm and 13mm is applicable to reaming in different situations.
Be equipped with double helix structure on the drilling rod 2, collecting pipe 3 parcel drilling rod 2 position for collect the osteogenesis material, in this embodiment, drilling rod 2 establishes to double helix structure and can produce local negative pressure through high-speed rotation, do benefit to the unidirectional derivation of osteogenesis material, double helix department of drilling rod 2 is equipped with protection device simultaneously, protection device and collecting pipe 3 joint, be used for avoiding the double helix structure of drilling rod 2 to cause the fish tail to the inner wall of collecting pipe 3, protection device is sleeve pipe 8, sleeve pipe 8 one end is equipped with external screw thread structure, collecting pipe 3 inner wall is equipped with the internal screw thread structure with sleeve pipe 8 external screw thread complex, and the direction that external screw thread screwed is the same with the direction of rotation of drilling rod 2, can play the effect that the anti-spin put drops, sleeve pipe 8 is located the other end of external screw thread simultaneously and is equipped with 4 holes, be used for avoiding causing the osteogenesis material to get into the not smooth problem of collecting pipe 3.
The rear end of the drill rod 2 is provided with a driving mechanism, the driving mechanism is in sliding connection with the drill rod 2, the drill rod 2 is positioned on the rotating shaft of the speed regulating motor 6, in the embodiment, the driving mechanism is the speed regulating motor 6, the rotating speed of the reaming bit 1 can be regulated according to actual conditions, the speed regulating motor 6 is in sliding connection with the drill rod 2, and the drill rod 2 is sleeved and locked through the slidable locking clamp cover 7 in the embodiment.
As shown in fig. 4, the bottom of the rear end of the collecting pipe 3 is provided with a water outlet 301 and a water inlet 302, the water inlet 302 is communicated with the tail of the drill rod 2, the water inlet 302 is used for filling physiological saline into the drill rod 2 through a pipeline, the contact part of the speed regulating motor 6 and the drill rod 2 is provided with a drill rod seal 201, and the drill rod seal 201 is used for sealing the tail of the drill rod 2 when the locking clamp cover 7 locks the drill rod 2 and the speed regulating motor 6, so that the physiological saline is prevented from overflowing from the rear of the hollow drill rod 2; the water outlet 301 is used for discharging the collected bone raw material mixed liquid, the water outlet 301 is communicated with the mixing stirring tank 4 through a pipeline, in the embodiment, a plastic pipeline is collected in the pipe 3, and the pipeline used by the water outlet 301 and the water inlet 302 is a colloid pipeline.
As shown in fig. 5 and 6, the mixing and stirring tank 4 includes an outer tank 402 and an inner tank 401, and a negative pressure suction device is arranged between the outer tank 402 and the inner tank 401, in this embodiment, the negative pressure suction device includes an electromagnetic valve, and the power source of the negative pressure suction device adopts a negative pressure machine equipped in an operating room, so that the structure and cost of the device can be reduced; the inner tank 401 contains an enrichment material and a mixing stirring device 403, the water outlet 301 is communicated with the outer tank 402 through a pipeline, and the bottom of the inner tank 401 is also provided with a turbulence device 404; the enrichment material in inner tank 401 is an allogeneic bone decalcification scaffold, the surface of which is modified by self-assembled polypeptide, in this embodiment, the self-assembled polypeptide comprises RADA16-I peptide and osteostatin peptide coupled to the C-terminal of the RADA16-I peptide sequence.
Mixing stirring device 403 includes (mixing) shaft and stirring leaf, and the stirring leaf is located the (mixing) shaft, and (mixing) shaft one end is connected with vortex device 404, and the other end is connected with external drive device, and in this embodiment, external drive device is for adopting the power supply that has in the operating room, does not need extra external source energy to intervene, guarantees stability and the security of equipment that like this can be better, and more is suitable for different operating room requirements, simultaneously reduce the cost.
The turbulence device 404 is a propeller blade, which rotates through a stirring shaft, and the propeller blade is mainly used for enabling the osteogenic material deposited at the bottom of the inner tank 401 to flow, so that the enrichment material is enriched with more substances required.
The waste liquid collecting tank 5 is used for collecting waste liquid in the mixing and stirring tank 4, a liquid outlet and a filtering device are further arranged at the bottom of the inner tank 401 of the mixing and stirring tank 4, the filtering device is a filter screen in the embodiment, the liquid outlet is covered, and the liquid outlet is communicated with the waste liquid collecting tank 5 through a pipeline.
The specific implementation process comprises the following steps: before using the marrow aspiration enrichment system, firstly, the normal saline is injected into the drill stem 2 through the water inlet 302, then the locking clamp cover 7 is adjusted, the speed regulating motor 6 and the drill stem 2 are locked, the tail of the drill stem 2 is plugged by the drill stem seal 201, the normal saline is prevented from overflowing, the speed regulating motor 6 is started, the reaming bit 1 and the drill stem 2 are controlled to carry out reaming, the drainage hole 101 on the reaming bit 1 is used for discharging the normal saline, the double-spiral structure on the drill stem 2 generates local negative pressure in the high-speed rotation process, the substances are led out unidirectionally, the collecting pipe 3 sleeved on the drill stem 2 collects the mixture of the led substances and the normal saline, and flows into the outer tank 402 of the mixing stirring tank 4 through the water outlet 301 and the water outlet 301, the negative pressure pipeline of the negative pressure aspiration system in the operating room is connected with the electromagnetic valve in the inner tank 401, so that the mixture flowing into the outer tank 402 is sucked into the inner tank 401 under negative pressure, the enrichment material in the inner tank 401 enriches the bone material in the mixture, and is fully enriched under the action of the mixing stirring device 403 and 404, so that the waste liquid can be discharged from the filter tank 5 after the waste liquid is collected in the collecting tank and the waste liquid is discharged from the filter tank through the bottom of the filter screen 401.
As shown in fig. 7, in another embodiment of the present invention, a method for constructing a high-activity bone based on a bone marrow aspiration enrichment system is further included, which is applied to the above-mentioned bone marrow aspiration enrichment system, and specifically includes:
the step of constructing an osteoinductive membrane: bone cement is used to fill the bone defect site to form an osteoinductive membrane.
In this embodiment, when bone cement is used to fill the bone defect site, fibrin and laminin are rapidly enriched on the surface of bone cement to form a membranous structure similar to periosteum, namely an osteoinductive membrane, wherein the inner layer of the osteoinductive membrane is an epithelial-like synovial membrane, and the outer layer of the osteoinductive membrane is composed of fibroblasts, myofibroblasts and type I collagen, so that the bone cement has certain mechanical strength, can form a closed cavity which keeps autologous morphology after being taken out, can not only maintain the tissue morphology of the transplanted bone, but also prevent the invasion of bacteria and the growth of surrounding soft tissues, protect the transplanted bone from being absorbed and promote bone healing.
Preparing an enrichment material: and placing the allogeneic bone decalcification stent in a prepared peptide solution for soaking, and flushing after the soaking is finished to obtain the allogeneic bone decalcification stent modified by the self-assembled polypeptide.
In the embodiment, firstly taking out an allogeneic bone decalcification stent, placing the allogeneic bone decalcification stent into a peptide solution for soaking, and preparing the peptide solution by dissolving fusion peptide with purity of more than or equal to 95% into a 20% sucrose solution until the final concentration of the solution is 1%, and performing ultrasonic treatment for 30 minutes to obtain the peptide solution; soaking the allogeneic bone decalcification scaffold in a peptide solution for 4-8 minutes, taking out, and flushing with a phosphate buffer salt solution with the pH value of 7.4 to obtain the allogeneic bone decalcification scaffold modified by the self-assembled polypeptide.
Enrichment: the prepared enrichment material is placed in the inner tank 401 of the mixing stirring tank 4 of the bone marrow aspiration enrichment system, and enrichment is performed according to a preset time.
In this embodiment, the allogeneic bone decalcification scaffold modified by the self-assembled polypeptide is placed in the inner tank 401 of the mixing stirring tank 4 of the bone marrow aspiration enrichment system, and the bone material is enriched according to the preset 30 minutes by the continuous stirring of the stirring shaft and the continuous turbulent flow of the propeller blade, so that the needed mesenchymal stem cells and the needed substances can be fully absorbed.
The step of constructing high-activity bone: and (5) discharging the waste liquid in the mixing stirring tank 4 after enrichment is completed, so as to obtain the constructed high-activity bone repair material.
In this embodiment, after enrichment is completed, the waste liquid is discharged through the filtering device and the liquid outlet of the outer tank 402 of the mixing and stirring tank 4, and the inner tank 401 is taken out to obtain the required high-activity bone-forming material.
And (3) repairing: taking out the bone cement, and implanting the constructed high-activity bone repair material into the induction membrane of the bone defect part.
In the embodiment, the time for forming the osteoinductive membrane is 8 weeks, bone cement is taken out after the osteoinductive membrane is formed, a high-activity bone repair material is implanted into a bone defect part, the osteoinductive membrane is a highly vascularized tissue, the thickness is 1-2 mm, the osteoinductive membrane is composed of type I glial cells, and fibroblasts are the main cell types; the inner surface of the membrane consists of epithelial-like cells, fibroblasts, myofibroblasts and collagen fiber bundles parallel to the surface of the membrane, and the osteoinductive membrane contains a plurality of growth factors such as vascular endothelial growth factor, transforming growth factor beta 1, bone morphogenetic protein-2 and the like with high concentration, and can promote the regeneration and repair of bones. The membrane protein extract also has the property of stimulating bone marrow cell proliferation and mesenchymal cell membrane differentiation. Therefore, the osteoinductive membrane has similar structure to periosteum, has more abundant blood supply and more abundant active factors necessary for bone growth than periosteum, and provides good biological conditions for bone healing for the reconstructed bone defect.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The foregoing is merely exemplary of the present application, and specific structures and features well known in the art will not be described in detail herein, so that those skilled in the art will be aware of all the prior art to which the present application pertains, and will be able to ascertain the general knowledge of the technical field in the application or prior art, and will not be able to ascertain the general knowledge of the technical field in the prior art, without using the prior art, to practice the present application, with the aid of the present application, to ascertain the general knowledge of the same general knowledge of the technical field in general purpose. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present application, and these should also be considered as the scope of the present application, which does not affect the effect of the implementation of the present application and the utility of the patent. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (8)
1. A bone marrow aspiration enrichment system, characterized by: the device comprises a reaming bit, a drill rod, a collecting pipe and a mixed stirring tank, wherein the reaming bit is detachably connected with the drill rod, the reaming bit and the drill rod are hollow, the reaming bit is provided with a plurality of drainage holes which are arranged at eccentric negative angles, the rear end of the drill rod is provided with a driving mechanism, the driving mechanism is in sliding connection with the drill rod, the collecting pipe wraps the drill rod, the rear end of the collecting pipe is provided with a water inlet and a water outlet, the water inlet is communicated with the tail part of the drill rod, the mixed stirring tank comprises an outer tank body and an inner tank body, a negative pressure suction device is arranged between the outer tank body and the inner tank body, the inner tank body contains enriched materials and a mixed stirring device, the water outlet is communicated with the outer tank body through a pipeline, and the bottom of the inner tank body is provided with a turbulence device;
the drill rod is provided with a double-screw structure, and a collecting pipe at the double-screw position of the drill rod is clamped with a protecting device.
2. A bone marrow aspiration enrichment system according to claim 1, wherein: the enrichment material is an allogeneic bone decalcification stent, and the surface of the allogeneic bone decalcification stent is modified by self-assembled polypeptide.
3. The bone marrow aspiration enrichment system according to claim 1, wherein the mixing and stirring device comprises a stirring shaft and stirring blades, wherein the stirring blades are positioned on the stirring shaft, one end of the stirring shaft is connected with the turbulence device, and the other end of the stirring shaft is connected with the external driving device.
4. A bone marrow aspiration enrichment system according to claim 1, wherein: the turbulence device is a propeller blade.
5. A bone marrow aspiration enrichment system according to claim 1, wherein: still include the waste liquid collection tank, the inner tank body bottom of mixing stirring jar is equipped with liquid outlet and filter equipment, the waste liquid collection tank passes through the pipe connection liquid outlet.
6. A bone marrow aspiration enrichment system according to claim 1, wherein: the reamer bit is a replaceable reamer bit, and the specification of the reamer bit is 10mm-13mm.
7. A bone marrow aspiration enrichment system according to claim 1, wherein: the driving mechanism is a speed regulating motor, and the drill rod is positioned on a rotating shaft of the speed regulating motor.
8. A method of constructing a highly active bone based on the bone marrow aspiration enrichment system of any one of claims 1-7, wherein: comprising the following steps:
Preparing an enrichment material: placing the allogeneic bone decalcification stent in a prepared peptide solution for soaking, and flushing after the soaking is finished to obtain the allogeneic bone decalcification stent modified by self-assembled polypeptide;
Enrichment: placing the prepared enrichment material into an inner tank body of a mixing stirring tank of a bone marrow aspiration enrichment system, and enriching according to preset time;
The step of constructing high-activity bone: and (5) discharging the waste liquid in the mixing stirring tank after the enrichment is finished, so as to obtain the constructed high-activity bone repair material.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105536067A (en) * | 2016-01-28 | 2016-05-04 | 中国人民解放军第三军医大学第一附属医院 | Method for building bone with high osteogenic activity |
| CN108025113A (en) * | 2015-07-31 | 2018-05-11 | 德普伊新特斯产品公司 | Method for preparing osteogenic bone graft |
| JP2021094270A (en) * | 2019-12-18 | 2021-06-24 | 株式会社ネクスト２１ | Bone marrow collection device |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60018712T2 (en) * | 1999-02-03 | 2006-03-16 | Synthes Ag Chur, Chur | SURGICAL DRILL |
| US6783533B2 (en) * | 2001-11-21 | 2004-08-31 | Sythes Ag Chur | Attachable/detachable reaming head for surgical reamer |
| CN210056200U (en) * | 2018-12-05 | 2020-02-14 | 齐国宝 | Bone cement injection device for vertebroplasty |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108025113A (en) * | 2015-07-31 | 2018-05-11 | 德普伊新特斯产品公司 | Method for preparing osteogenic bone graft |
| CN105536067A (en) * | 2016-01-28 | 2016-05-04 | 中国人民解放军第三军医大学第一附属医院 | Method for building bone with high osteogenic activity |
| JP2021094270A (en) * | 2019-12-18 | 2021-06-24 | 株式会社ネクスト２１ | Bone marrow collection device |
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