CN113880612A - Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application - Google Patents
Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application Download PDFInfo
- Publication number
- CN113880612A CN113880612A CN202111243933.XA CN202111243933A CN113880612A CN 113880612 A CN113880612 A CN 113880612A CN 202111243933 A CN202111243933 A CN 202111243933A CN 113880612 A CN113880612 A CN 113880612A
- Authority
- CN
- China
- Prior art keywords
- seaweed
- liquid fertilizer
- enzymolysis
- organic liquid
- microbial agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001474374 Blennius Species 0.000 title claims abstract description 193
- 239000003337 fertilizer Substances 0.000 title claims abstract description 80
- 239000007788 liquid Substances 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000000855 fermentation Methods 0.000 claims abstract description 42
- 230000004151 fermentation Effects 0.000 claims abstract description 42
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 40
- 230000000813 microbial effect Effects 0.000 claims abstract description 37
- 102000004190 Enzymes Human genes 0.000 claims abstract description 34
- 108090000790 Enzymes Proteins 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 150000001875 compounds Chemical class 0.000 claims abstract description 24
- 239000002002 slurry Substances 0.000 claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 19
- 241000195474 Sargassum Species 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 10
- 239000000413 hydrolysate Substances 0.000 claims abstract description 10
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 238000010298 pulverizing process Methods 0.000 claims abstract description 5
- 229940088598 enzyme Drugs 0.000 claims description 32
- 238000002791 soaking Methods 0.000 claims description 20
- 239000004365 Protease Substances 0.000 claims description 13
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 4
- 102000004317 Lyases Human genes 0.000 claims description 4
- 108090000856 Lyases Proteins 0.000 claims description 4
- 102000004316 Oxidoreductases Human genes 0.000 claims description 4
- 108090000854 Oxidoreductases Proteins 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 4
- 108010059820 Polygalacturonase Proteins 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 4
- 241000223261 Trichoderma viride Species 0.000 claims description 4
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 102000004157 Hydrolases Human genes 0.000 claims description 3
- 108090000604 Hydrolases Proteins 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 235000021190 leftovers Nutrition 0.000 claims description 3
- 240000004482 Withania somnifera Species 0.000 claims description 2
- 235000001978 Withania somnifera Nutrition 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- 241000196253 Ulva prolifera Species 0.000 claims 1
- 230000001461 cytolytic effect Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 14
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 239000004480 active ingredient Substances 0.000 abstract description 4
- 239000000783 alginic acid Substances 0.000 description 24
- 235000010443 alginic acid Nutrition 0.000 description 24
- 229960001126 alginic acid Drugs 0.000 description 24
- 229920000615 alginic acid Polymers 0.000 description 24
- 150000004781 alginic acids Chemical class 0.000 description 24
- 230000035784 germination Effects 0.000 description 23
- 238000002156 mixing Methods 0.000 description 20
- 238000003756 stirring Methods 0.000 description 20
- 235000013311 vegetables Nutrition 0.000 description 16
- 239000008213 purified water Substances 0.000 description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 10
- 238000007605 air drying Methods 0.000 description 10
- 238000004140 cleaning Methods 0.000 description 10
- 230000001276 controlling effect Effects 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 10
- 108091005804 Peptidases Proteins 0.000 description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 9
- 235000019419 proteases Nutrition 0.000 description 9
- 240000008067 Cucumis sativus Species 0.000 description 7
- 241000227653 Lycopersicon Species 0.000 description 7
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 239000003085 diluting agent Substances 0.000 description 7
- 235000009849 Cucumis sativus Nutrition 0.000 description 6
- 230000003203 everyday effect Effects 0.000 description 6
- 238000005286 illumination Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 239000013543 active substance Substances 0.000 description 5
- 238000007865 diluting Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 241000196252 Ulva Species 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000199919 Phaeophyceae Species 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/40—Treatment of liquids or slurries
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a method for preparing a seaweed biological organic liquid fertilizer, which comprises the following steps: (1) pretreatment of seaweed: pulverizing Sargassum raw material, and adding water to obtain Sargassum slurry; (2) carrying out enzymolysis reaction by using a compound enzyme: adding compound enzyme, performing enzymolysis at pH 4-8 and 40-75 deg.C for 2-25 hr with addition amount of 1-6% of the Sargassum slurry to obtain Sargassum enzymolysis solution; (3) fermenting by using a mixed microbial agent: and (3) adding the seaweed enzymatic hydrolysate obtained in the step (2) into a mixed microbial agent, fermenting for 0-72 hours, wherein the inoculation amount is 0.1-0.6%, the bottling amount is 20-60mL/100mL, the fermentation temperature is 35-60 ℃, and the initial pH is 5.5-7.5. The invention also provides the seaweed biological organic liquid fertilizer prepared by the method and application thereof. The invention improves the utilization rate of nutrient substances in the seaweed, fully retains the effective active ingredients in the seaweed, does not introduce chemical substances, and improves the quality and the fertilizing effect of the seaweed liquid fertilizer.
Description
Technical Field
The invention relates to the field of fertilizer manufacturing, in particular to a method for preparing a seaweed biological organic liquid fertilizer, the seaweed biological organic liquid fertilizer and application.
Background
China has 1.8-kilo-kilometer coastline, the aquatic resources are rich, and Chinese people begin to harvest and utilize seaweed since ancient times. The seaweed is edible and has great application potential in many fields such as functional food, marine medicine, animal feed and the like because the seaweed is rich in macroelements, plant growth regulating substances (cytokinins, auxins, cytokinins, abscisic acid and gibberellins), highly unsaturated fatty acids and the like.
The Seaweed Liquid Fertilizer (Seaweed Liquid fertilzer) is a Liquid Fertilizer formed by breaking the cell walls of large-scale Seaweed by adopting a special production process and releasing the contents of the cells, and is a novel pollution-free green Fertilizer. The main raw materials for producing the seaweed fertilizer are brown algae, including kelp, gulfweed, kelp, and Ascophyllum nodosum. The research of applying the seaweed fertilizer to crops and vegetables is more, and the seaweed fertilizer can improve the germination rate of seeds, the yield and the quality of the crops, the stress resistance of the crops, the disease and insect resistance and the like.
At present, the seaweed liquid fertilizer is prepared by an acid method, an alkali method or an acid-alkali mixing method, the methods are usually carried out under the conditions of strong acid, alkali and high temperature, biological source active substances in seaweed cells can be damaged, the quality of residual chemical substances is high, the residual chemical substances are not easy to remove, and the quality and the fertilizing effect of the seaweed liquid fertilizer are influenced. With the wide application of biotechnology in recent years, the biological enzymolysis method becomes one of the preparation processes of the seaweed liquid fertilizer. Seaweed is a key factor for hindering the release of active substances in seaweed cells due to the complex structure and special toughness of the cell walls of the seaweed. How to efficiently extract and utilize nutrient substances in the seaweed and ensure that various active substances are not damaged in the processing process is a bottleneck in the field of seaweed fertilizer production and processing.
Disclosure of Invention
The invention aims to solve the technical problems that the preparation method of the seaweed liquid fertilizer in the prior art causes high quality of residual chemicals, is not easy to remove, influences the quality and the fertilizing effect of the seaweed liquid fertilizer, and has low utilization rate of nutrient substances in seaweed and damages active substances in the processing process. The invention provides a method for preparing a seaweed bio-organic liquid fertilizer, the seaweed bio-organic liquid fertilizer and application thereof, which can improve the utilization rate of nutrient substances in seaweed, fully release the content of seaweed cells, fully retain the effective active ingredients in the seaweed, and greatly improve the quality and the fertilizing effect of the seaweed liquid fertilizer without introducing chemical substances.
In order to solve the technical problems, the embodiment of the invention discloses a method for preparing a seaweed biological organic liquid fertilizer, which comprises the following steps:
(1) pretreatment of seaweed: pulverizing Sargassum raw material, and adding water to obtain Sargassum slurry;
(2) carrying out enzymolysis reaction by using a compound enzyme: taking the seaweed slurry obtained in the step (1), adding a compound enzyme, wherein the enzymolysis condition is enzymolysis pH of 4-8, the enzymolysis temperature is 40-75 ℃, and according to the weight percentage, the compound enzyme is added in an amount of 1-6% of the seaweed slurry, and the enzymolysis time is 2-25 hours, so as to obtain seaweed enzymolysis liquid;
(3) fermenting by using a mixed microbial agent: and (3) adding a mixed microbial agent into the seaweed enzymatic hydrolysate obtained in the step (2) for fermentation, wherein the fermentation time is 0-72 hours, the inoculation amount of the mixed microbial agent is 0.1-0.6% of the seaweed enzymatic hydrolysate according to the weight percentage, the bottling amount of the mixed microbial agent is 20-60mL/100mL, the fermentation temperature is 35-60 ℃, and the initial fermentation pH is 5.5-7.5.
According to another specific embodiment of the invention, the embodiment of the invention discloses a method for preparing the seaweed bio-organic liquid fertilizer, the compound enzyme agent comprises cellulose hydrolase, pectinase, papain, oxidoreductase, lyase and xylanase, and the mixed microbial agent comprises bacillus licheniformis, saccharomyces cerevisiae and trichoderma viride.
According to another specific embodiment of the invention, the embodiment of the invention discloses a method for preparing a seaweed biological organic liquid fertilizer, wherein a compound enzyme agent comprises 3-3.5g/L of cellulose hydrolase, 1-1.5g/L of pectinase, 0.8-8g/L of papain, 0.5-1g/L of oxidoreductase, 1-2.5g/L of lyase and 2-2.5g/L of xylanase;
the mixed microbial agent is 1-2 g/L of bacillus licheniformis, 0.6-1.2 g/L of saccharomyces cerevisiae and 1-1.5g/L of trichoderma viride.
According to another embodiment of the invention, the embodiment of the invention discloses a method for preparing a seaweed biological organic liquid fertilizer, wherein the seaweed is processed leftovers of kelp naturally grown in a Withania hookeri area, enteromorpha or gulfweed.
According to another embodiment of the invention, the invention discloses a method for preparing the seaweed bio-organic liquid fertilizer, in the step (1), the seaweed raw material is crushed to 100-200 meshes.
According to another embodiment of the invention, the embodiment of the invention discloses a method for preparing a seaweed bio-organic liquid fertilizer, which comprises the following steps:
(1) pretreatment of seaweed: pulverizing Sargassum raw material, and adding water to obtain Sargassum slurry;
(2) carrying out enzymolysis reaction by using a compound enzyme: taking the seaweed slurry obtained in the step (1), adding a compound enzyme, wherein the enzymolysis condition is enzymolysis pH of 6.5, the enzymolysis temperature is 60 ℃, and according to the weight percentage, the compound enzyme is added in an amount of 4% of the seaweed slurry, and the enzymolysis time is 6 hours, so as to obtain seaweed enzymolysis liquid;
(3) fermenting by using a mixed microbial agent: and (3) adding a mixed microbial agent into the seaweed enzymatic hydrolysate obtained in the step (2) for fermentation, wherein the fermentation time is 36 hours, the inoculation amount of the mixed microbial agent is 0.3 percent of that of the seaweed enzymatic hydrolysate according to the weight percentage, the bottling amount of the mixed microbial agent is 40mL/100mL, the fermentation temperature is 45 ℃, and the initial fermentation pH is 6.5.
The embodiment of the invention also discloses the seaweed bio-organic liquid fertilizer prepared by the method for preparing the seaweed bio-organic liquid fertilizer.
The embodiment of the invention also discloses application of the seaweed bio-organic liquid fertilizer in the field of agricultural planting.
According to another specific embodiment, the embodiment of the invention discloses application of the seaweed bio-organic liquid fertilizer in the field of agricultural planting, and when the seaweed bio-organic liquid fertilizer is used, the seaweed bio-organic liquid fertilizer is diluted by water by 200-fold and 1000-fold for seed soaking, seedling raising, leaf surface spraying or irrigation.
Compared with the prior art, the invention has the following technical effects:
the utilization rate of nutrient substances in the seaweed is improved, the content of seaweed cells is fully released, the effective active ingredients in the seaweed are fully reserved, no chemical substance is introduced, and the quality of the seaweed liquid fertilizer and the fertilizing effect are greatly improved.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure. While the invention will be described in conjunction with the preferred embodiments, it is not intended that features of the invention be limited to these embodiments. On the contrary, the invention is described in connection with the embodiments for the purpose of covering alternatives or modifications that may be extended based on the claims of the present invention. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be practiced without these particulars. Moreover, some of the specific details have been left out of the description in order to avoid obscuring or obscuring the focus of the present invention. It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The seaweed related in each embodiment of the invention is leftovers after processing kelp naturally growing in a tobacco Weihai area, enteromorpha and gulfweed; the Kate 98 green-core cucumber, the slow rape of April and the fine 206 tomato seeds used in the embodiment of the invention are purchased from seed stations in the tobacco stage market; the seaweed raw material is crushed by using an ultra-speed seaweed crusher, which is a dry kelp crusher in patent CN201810852035.6 developed by the applicant.
The alginic acid content in the enzymolysis liquid is measured by a spectrophotometric method.
First, compound enzyme enzymolysis reaction embodiment
Example 1
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding compound enzyme (3mL) which is 3% of the weight of the seaweed homogenate, adding dilute hydrochloric acid to adjust the pH value to 6, controlling the enzymolysis temperature to be 60 ℃ and the enzymolysis time to be 6h, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 30.189 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 2
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding complex enzyme (4mL) with the weight of 4% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 6, controlling the enzymolysis temperature to be 65 ℃ and the enzymolysis time to be 8h, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 38.142 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 3
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding a complex enzyme (5mL) with the weight of 5% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 6, controlling the enzymolysis temperature to be 70 ℃ and the enzymolysis time to be 10 hours, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 36.415 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 4
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding a complex enzyme (5mL) with the weight of 5% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 6.5, controlling the enzymolysis temperature to be 60 ℃ and the enzymolysis time to be 8h, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 40.125 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 5
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding complex enzyme (3mL) which is 3% of the weight of the seaweed homogenate, adding dilute hydrochloric acid to adjust the pH value to 6.5, controlling the enzymolysis temperature to be 65 ℃ and the enzymolysis time to be 10 hours, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 37.014 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 6
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding complex enzyme (4mL) with the weight of 4% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 6.5, controlling the enzymolysis temperature to be 70 ℃ and the enzymolysis time to be 6h, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 32.287 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 7
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding complex enzyme (4mL) with the weight of 4% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 7, controlling the enzymolysis temperature to be 60 ℃ and the enzymolysis time to be 10 hours, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 32.017 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 8
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding a complex enzyme (5mL) with the weight of 5% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 7, controlling the enzymolysis temperature to be 65 ℃ and the enzymolysis time to be 6 hours, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 36.178 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Example 9
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding complex enzyme (3mL) which is 3% of the weight of the seaweed homogenate, adding dilute hydrochloric acid to adjust the pH value to 7, controlling the enzymolysis temperature to be 70 ℃ and the enzymolysis time to be 8h, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 38.237 mg/mL;
(3) and (5) mixing the microbial agent for fermentation.
Second, fermentation example of Mixed microbial Agents
Example 10
A method for preparing a seaweed biological organic liquid fertilizer comprises the following specific steps:
(1) cleaning fresh seaweed, air drying, crushing to 100-200 meshes by using an overspeed seaweed crusher, mixing with distilled water according to a ratio of 1:10(W/V), and fully and uniformly stirring to prepare seaweed homogenate;
(2) taking 100mL of seaweed homogenate, adding a complex enzyme (5mL) with the weight of 5% of the seaweed slurry, adding dilute hydrochloric acid to adjust the pH value to 6.5, controlling the enzymolysis temperature to be 60 ℃ and the enzymolysis time to be 8h, stirring for enzymolysis, taking supernate after the reaction is finished, and determining the alginic acid content, wherein the determined alginic acid content is 40.125 mg/mL;
(3) adjusting the pH of the seaweed enzymolysis liquid to about 7.0, carrying out damp-heat sterilization for 20min by high-pressure steam at 121 ℃, adding a mixed microbial agent with the weight of 0.3 percent of that of the seaweed enzymolysis liquid for fermentation, carrying out fermentation by using the mixed microbial agent with the fermentation bottling amount of 40mL/100mL, the fermentation temperature of 45 ℃ and the initial fermentation pH of 6.5, and measuring the alginic acid content in the fermentation liquid to be 26.354mg/mL after the fermentation is finished.
Application example of biological organic liquid fertilizer containing seaweed
Application example 1
Diluting the prepared seaweed biological organic liquid fertilizer by 200 times, soaking seeds of Kate 98 green-core cucumbers, March slow rape and fine 206 tomatoes respectively, soaking the seeds for 12 hours, washing the seeds for 3 times by purified water after soaking, uniformly placing the seeds in culture dishes paved with double layers of filter paper, placing 50 vegetable seeds in each culture dish, enabling each seed to be treated in parallel for 3 times, covering the seeds with gauze, placing the seeds in an illumination incubator at 25 ℃, spraying the purified water every day to keep moist, observing and recording the germination conditions of the vegetable seeds during the culture period, and calculating the germination rate of the seeds, wherein the influence of the seaweed biological organic liquid fertilizer diluent on the germination rate of the seeds is shown in Table 1.
Application example 2
Diluting the prepared seaweed biological organic liquid fertilizer by 400 times, soaking seeds of Kate 98 green-core cucumbers, March slow rape and fine 206 tomatoes respectively, soaking the seeds for 12 hours, washing the seeds for 3 times by purified water after soaking, uniformly placing the seeds in culture dishes paved with double layers of filter paper, placing 50 vegetable seeds in each culture dish, enabling each seed to be treated in parallel for 3 times, covering the seeds with gauze, placing the seeds in an illumination incubator at 25 ℃, spraying the purified water every day to keep moist, observing and recording the germination conditions of the vegetable seeds during the culture period, and calculating the germination rate of the seeds, wherein the influence of the seaweed biological organic liquid fertilizer diluent on the germination rate of the seeds is shown in Table 1.
Application example 3
Diluting the prepared seaweed biological organic liquid fertilizer by 600 times, soaking seeds of Kate 98 green-core cucumbers, March slow rape and fine 206 tomatoes respectively, soaking the seeds for 12 hours, washing the seeds for 3 times by purified water after soaking, uniformly placing the seeds in culture dishes paved with double layers of filter paper, placing 50 vegetable seeds in each culture dish, enabling each seed to be treated in parallel for 3 times, covering the seeds with gauze, placing the seeds in an illumination incubator at 25 ℃, spraying the purified water every day to keep moist, observing and recording the germination conditions of the vegetable seeds during the culture period, and calculating the germination rate of the seeds, wherein the influence of the seaweed biological organic liquid fertilizer diluent on the germination rate of the seeds is shown in Table 1.
Application example 4
Diluting the prepared seaweed biological organic liquid fertilizer by 800 times, soaking seeds of Kate 98 green-core cucumbers, March slow rape and fine 206 tomatoes respectively, soaking the seeds for 12 hours, washing the seeds for 3 times by purified water after soaking, uniformly placing the seeds in culture dishes paved with double layers of filter paper, placing 50 vegetable seeds in each culture dish, enabling each seed to be treated in parallel for 3 times, covering the seeds with gauze, placing the seeds in an illumination incubator at 25 ℃, spraying the purified water every day to keep moist, observing and recording the germination conditions of the vegetable seeds during the culture period, and calculating the germination rate of the seeds, wherein the influence of the seaweed biological organic liquid fertilizer diluent on the germination rate of the seeds is shown in Table 1.
Application example 5
Diluting the prepared seaweed biological organic liquid fertilizer by 1000 times, soaking seeds of Kate 98 green-core cucumbers, March slow rape and fine 206 tomatoes respectively, soaking the seeds for 12 hours, washing the seeds for 3 times by purified water after soaking, uniformly placing the seeds in culture dishes paved with double layers of filter paper, placing 50 vegetable seeds in each culture dish, enabling each seed to be treated in parallel for 3 times, covering the seeds with gauze, placing the seeds in an illumination incubator at 25 ℃, spraying the purified water every day to keep moist, observing and recording the germination conditions of the vegetable seeds during the culture period, and calculating the germination rate of the seeds, wherein the influence of the seaweed biological organic liquid fertilizer diluent on the germination rate of the seeds is shown in Table 1.
Comparative example
Soaking seeds of Kate 98 green-core cucumbers, March slow rapes and fine 206 tomatoes in clean water respectively, soaking the seeds for 12 hours, washing the seeds for 3 times by purified water after soaking, uniformly placing the seeds in culture dishes paved with double layers of filter paper, placing 50 vegetable seeds in each culture dish, placing 3 treated seeds in parallel, covering the seeds with gauze, placing the seeds in an illumination incubator at 25 ℃, spraying the purified water every day to keep the seeds moist, observing and recording the germination conditions of the vegetable seeds during the culture, and calculating the germination rate of the seeds, wherein the influence of the seaweed biological organic liquid fertilizer diluent on the germination rate of the seeds is shown in Table 1.
TABLE 1 Effect of different seaweed bioorganic liquid fertilizer diluents and clear water on vegetable seed germination (%)
As can be seen from the table 1, compared with the comparative example, the seaweed liquid fertilizer with each dilution ratio has the effect of promoting the germination of the seeds of three kinds of vegetable seeds, and the seaweed liquid fertilizer mainly has the effect of promoting the germination of the seeds because the seaweed fertilizer contains various natural active substances and micromolecular bioactive substances in cells of the seaweed after wall breaking. Wherein the 600 times diluted fermented seaweed liquid fertilizer is most suitable for seed germination.
According to the invention, kelp, enteromorpha, gulfweed and the like are taken as raw materials, enzymolysis is carried out by using the compound enzyme, the enzymolysis pH, the enzymolysis temperature, the enzyme addition amount and the enzymolysis reaction time are studied, and the optimal process conditions for obtaining the compound enzyme enzymolysis seaweed are as follows: the pH value of enzymolysis is 6.5, the enzymolysis temperature is 60 ℃, the enzyme addition amount is 4 percent, and the enzymolysis time is 6 hours. Further fermenting the seaweed enzymatic hydrolysate by using the microbial agent, and obtaining the optimal fermentation process conditions by optimizing the fermentation process: the fermentation time is 36h, the inoculation amount of the microbial inoculum is 0.3%, the fermentation bottling amount is 40mL/100mL, the fermentation temperature is 45 ℃, and the initial fermentation pH is 6.5. The seaweed liquid fertilizer diluted by 600 times can obviously promote the germination of three vegetable seeds, and the germination rates are respectively improved by 30.99%, 19.77% and 19.94%.
The enzymolysis pH can affect the stability of the protease, the combination condition of protease molecules and enzymolysis substrates and the influence on each dissociation group of the protease, thereby affecting the enzymolysis reaction efficiency; for the protease, the activity of the protease is lower when the enzymolysis temperature is too low, but the activity of the protease is partially or completely lost when the enzymolysis temperature is too high, so that the proper enzymolysis reaction temperature is favorable for the hydrolysis of the seaweed; in an enzymolysis reaction system, when the addition amount of protease is too small, the substrate seaweed cannot be completely enzymolyzed, so the effect is poor; when the protease is added too much, all the substrate seaweeds are subjected to enzymolysis, and meanwhile, the enzymolysis products can inhibit the activity of the protease; the alginic acid content data of example 10 shows that the microbial agent can be degraded into small molecular substances by fermentation by utilizing alginic acid in the enzymolysis liquid.
The main preparation difficulty of producing the seaweed liquid fertilizer by using natural seaweed as a raw material is that macromolecular substances in the seaweed are effectively degraded, and nutrient elements and bioactive substances in the seaweed are retained to the maximum extent. At present, the process for preparing the seaweed fertilizer in China mainly adopts physical crushing, chemical extraction and the like, and usually destroys effective active ingredients in the seaweed, thus seriously affecting the fertilizer efficiency. The preparation of the seaweed fertilizer by a biological method is one of the most effective processes in modern industrial production. In the research, a compound enzymolysis and coupling microbial fermentation technology is adopted, so that the problem of seaweed wall breaking is effectively solved, the content of seaweed cells is fully released, and effective bioactive components in the seaweed are reserved.
While the invention has been described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that the foregoing is a more particular description of the invention than is possible with reference to the specific embodiments, which are not to be construed as limiting the invention. Various changes in form and detail, including simple deductions or substitutions, may be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (9)
1. A method for preparing a seaweed biological organic liquid fertilizer is characterized by comprising the following steps:
(1) pretreatment of seaweed: pulverizing Sargassum raw material, and adding water to obtain Sargassum slurry;
(2) carrying out enzymolysis reaction by using a compound enzyme: taking the seaweed slurry obtained in the step (1), adding a compound enzyme, wherein the enzymolysis condition is enzymolysis pH of 4-8, the enzymolysis temperature is 40-75 ℃, and according to the weight percentage, the compound enzyme is added in an amount of 1-6% of the seaweed slurry, and the enzymolysis time is 2-25 hours, so as to obtain seaweed enzymolysis liquid;
(3) fermenting by using a mixed microbial agent: and (3) adding a mixed microbial agent into the seaweed enzymatic hydrolysate obtained in the step (2) for fermentation, wherein the fermentation time is 0-72 hours, the inoculation amount of the mixed microbial agent is 0.1-0.6% of the seaweed enzymatic hydrolysate according to the weight percentage, the bottling amount of the mixed microbial agent is 20-60mL/100mL, the fermentation temperature is 35-60 ℃, and the initial fermentation pH is 5.5-7.5.
2. The method for preparing the seaweed bio-organic liquid fertilizer as claimed in claim 1, wherein the compound enzyme agent comprises cellulolytic enzyme, pectinase, papain, oxidoreductase, lyase and xylanase, and the compound microbial agent comprises bacillus licheniformis, saccharomyces cerevisiae and trichoderma viride.
3. The method for preparing the seaweed biological organic liquid fertilizer as claimed in claim 2, wherein the compound enzyme agent is cellulose hydrolase 3-3.5g/L, pectinase 1-1.5g/L, papain 0.8-8g/L, oxidoreductase 0.5-1g/L, lyase 1-2.5g/L, xylanase 2-2.5 g/L;
the mixed microbial agent is 1-2 g/L of bacillus licheniformis, 0.6-1.2 g/L of saccharomyces cerevisiae and 1-1.5g/L of trichoderma viride.
4. The method for preparing a seaweed bio-organic liquid fertilizer as claimed in claim 1, wherein the seaweed is processed leftovers of kelp naturally grown in the Withania hookeri, Enteromorpha prolifera or gulfweed.
5. The method for preparing liquid fertilizer of seaweed bio-organic as claimed in claim 1, wherein in step (1), the seaweed raw material is pulverized to 100-200 mesh.
6. The method for preparing the seaweed bio-organic liquid fertilizer as claimed in claim 1, comprising the steps of:
(1) pretreatment of seaweed: pulverizing Sargassum raw material, and adding water to obtain Sargassum slurry;
(2) carrying out enzymolysis reaction by using a compound enzyme: taking the seaweed slurry obtained in the step (1), adding a compound enzyme, wherein the enzymolysis condition is enzymolysis pH of 6.5, the enzymolysis temperature is 60 ℃, and according to the weight percentage, the compound enzyme is added in an amount of 4% of the seaweed slurry, and the enzymolysis time is 6 hours, so as to obtain seaweed enzymolysis liquid;
(3) fermenting by using a mixed microbial agent: and (3) adding a mixed microbial agent into the seaweed enzymatic hydrolysate obtained in the step (2) for fermentation, wherein the fermentation time is 36 hours, the inoculation amount of the mixed microbial agent is 0.3 percent of that of the seaweed enzymatic hydrolysate according to the weight percentage, the bottling amount of the mixed microbial agent is 40mL/100mL, the fermentation temperature is 45 ℃, and the initial fermentation pH is 6.5.
7. A seaweed bio-organic liquid fertilizer prepared by the method for preparing the seaweed bio-organic liquid fertilizer as claimed in any one of claims 1 to 6.
8. The application of the seaweed bioorganic liquid fertilizer in the field of agricultural planting according to claim 7.
9. The use as claimed in claim 8, wherein the seaweed bioorganic liquid fertilizer is diluted by 200-fold and 1000-fold with water for seed soaking, seedling raising, foliage spraying or irrigation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243933.XA CN113880612A (en) | 2021-10-25 | 2021-10-25 | Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243933.XA CN113880612A (en) | 2021-10-25 | 2021-10-25 | Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113880612A true CN113880612A (en) | 2022-01-04 |
Family
ID=79014155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111243933.XA Pending CN113880612A (en) | 2021-10-25 | 2021-10-25 | Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113880612A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114989984A (en) * | 2022-07-25 | 2022-09-02 | 青岛科光生物技术有限公司 | Method for preparing seaweed extract by enzymolysis |
| CN115160040A (en) * | 2022-09-08 | 2022-10-11 | 山东嘉禾海洋生物科技有限公司 | Alginic acid water-soluble fertilizer and preparation method thereof |
| CN116444309A (en) * | 2023-03-21 | 2023-07-18 | 山东百欧生物科技有限公司 | Microbial organic seaweed fertilizer and preparation method thereof |
| CN116874321A (en) * | 2023-07-14 | 2023-10-13 | 广州优卡思农业技术有限公司 | Method for preparing seaweed liquid fertilizer by using seaweed |
| CN117658725A (en) * | 2023-11-27 | 2024-03-08 | 山东思科生物科技有限公司 | Seaweed compound water-soluble fertilizer and preparation method and application thereof |
| CN117736029A (en) * | 2023-11-30 | 2024-03-22 | 中山市津海生物技术有限公司 | A method for producing seaweed extract |
| CN118026742A (en) * | 2024-03-12 | 2024-05-14 | 江苏海洋大学 | Preparation method and application of microbial seaweed fertilizer |
| CN118026771A (en) * | 2024-03-05 | 2024-05-14 | 江苏海洋大学 | Microbial seaweed fertilizer, preparation method thereof and application of microbial seaweed fertilizer as tobacco fertilizer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731176A (en) * | 2012-06-28 | 2012-10-17 | 威海市世代海洋生物科技有限公司 | Production method for seaweed bio-composite organic liquid fertilizer |
| CN104403980A (en) * | 2014-12-11 | 2015-03-11 | 中国农业大学 | Complex bacterial preparation and method for preparing cellulose by using complex bacterial preparation |
| CN110183266A (en) * | 2019-04-17 | 2019-08-30 | 青岛明月蓝海生物科技有限公司 | A kind of small molecule alginic acid microbial manure |
| CN112573973A (en) * | 2020-12-25 | 2021-03-30 | 海城市盛世鸿翔有机肥制造有限公司 | Seaweed enzymolysis seedling raising fertilizer, and preparation method and application thereof |
-
2021
- 2021-10-25 CN CN202111243933.XA patent/CN113880612A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731176A (en) * | 2012-06-28 | 2012-10-17 | 威海市世代海洋生物科技有限公司 | Production method for seaweed bio-composite organic liquid fertilizer |
| CN104403980A (en) * | 2014-12-11 | 2015-03-11 | 中国农业大学 | Complex bacterial preparation and method for preparing cellulose by using complex bacterial preparation |
| CN110183266A (en) * | 2019-04-17 | 2019-08-30 | 青岛明月蓝海生物科技有限公司 | A kind of small molecule alginic acid microbial manure |
| CN112573973A (en) * | 2020-12-25 | 2021-03-30 | 海城市盛世鸿翔有机肥制造有限公司 | Seaweed enzymolysis seedling raising fertilizer, and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王玥琛 等: "大型海藻肥料化应用", 《热带农业工程》 * |
| 董华芳等: "海藻有机肥对草莓叶绿素和果实可溶性固形物的影响", 《现代农业科技》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114989984A (en) * | 2022-07-25 | 2022-09-02 | 青岛科光生物技术有限公司 | Method for preparing seaweed extract by enzymolysis |
| CN114989984B (en) * | 2022-07-25 | 2023-10-13 | 青岛科光生物技术有限公司 | Method for preparing seaweed extract by enzymolysis |
| CN115160040A (en) * | 2022-09-08 | 2022-10-11 | 山东嘉禾海洋生物科技有限公司 | Alginic acid water-soluble fertilizer and preparation method thereof |
| CN116444309A (en) * | 2023-03-21 | 2023-07-18 | 山东百欧生物科技有限公司 | Microbial organic seaweed fertilizer and preparation method thereof |
| CN116874321A (en) * | 2023-07-14 | 2023-10-13 | 广州优卡思农业技术有限公司 | Method for preparing seaweed liquid fertilizer by using seaweed |
| CN117658725A (en) * | 2023-11-27 | 2024-03-08 | 山东思科生物科技有限公司 | Seaweed compound water-soluble fertilizer and preparation method and application thereof |
| CN117736029A (en) * | 2023-11-30 | 2024-03-22 | 中山市津海生物技术有限公司 | A method for producing seaweed extract |
| CN118026771A (en) * | 2024-03-05 | 2024-05-14 | 江苏海洋大学 | Microbial seaweed fertilizer, preparation method thereof and application of microbial seaweed fertilizer as tobacco fertilizer |
| CN118026742A (en) * | 2024-03-12 | 2024-05-14 | 江苏海洋大学 | Preparation method and application of microbial seaweed fertilizer |
| CN118026742B (en) * | 2024-03-12 | 2024-08-30 | 江苏海洋大学 | Preparation method and application of microbial seaweed fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113880612A (en) | Method for preparing seaweed biological organic liquid fertilizer, seaweed biological organic liquid fertilizer and application | |
| US8530220B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN108383559A (en) | A kind of method that microbial fermentation enzymolysis prepares organic liquid seaweed extract | |
| CN105936881B (en) | One kind is for alignic thermophilic sugared bacillus and its application method of degrading | |
| CN106748195B (en) | The fertilizer and its preparation technology of a kind of growth substance containing natural plants | |
| CN103540556B (en) | Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer | |
| CN102898195B (en) | Method for preparing biological fertilizers from corn soaking liquid | |
| CN109511465A (en) | A kind of oyster mushroom cultivating in bag method based on corncob | |
| CN110367023B (en) | Method for promoting lignification of spray-sowed plants | |
| CN110734322A (en) | Method for assisting fermentation of crop straws by alginate | |
| CN110981635A (en) | Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof | |
| CN103073337A (en) | Method for enhancing soil fertility by prompting straw degradation with cellulase | |
| CN110845273A (en) | Preparation method and application of shell powder organic fertilizer biological conditioner | |
| CN111670779A (en) | Banana tissue culture seedling improvement matrix and preparation method and application thereof | |
| CN111034407A (en) | Microbial utilization technology for returning rice straws to field in northeast region | |
| CN105505799A (en) | Fermentation method and application of trichoderma viride mutant strains | |
| CN112143770B (en) | Marine rhodotorula and application thereof in producing beta-carotene by taking straw as raw material | |
| CN116573959A (en) | Preparation method of special trichoderma viride fertilizer for young garlic bulbs | |
| CN115215700A (en) | Liquid fertilizer based on biomass fermentation and preparation method thereof | |
| CN111116263B (en) | Application of nano-chitin in improving utilization rate of fertilizer | |
| CN115197018A (en) | Organic water-soluble fertilizer and preparation method thereof | |
| CN106047775A (en) | Stain of bacillus megatherium for producing cellulase and application method thereof | |
| CN110754245A (en) | Preparation method and application of kelp enzymatic hydrolysate | |
| CN105746520A (en) | Application of nano-chitin in improvement of yield and quality of tobaccos | |
| CN111285739A (en) | Tobacco-planting soil remediation organic and compound fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220104 |