CN113359287A - Angle-adjustable rotary objective and microscope - Google Patents
Angle-adjustable rotary objective and microscope Download PDFInfo
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- CN113359287A CN113359287A CN202110654766.1A CN202110654766A CN113359287A CN 113359287 A CN113359287 A CN 113359287A CN 202110654766 A CN202110654766 A CN 202110654766A CN 113359287 A CN113359287 A CN 113359287A
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- 239000000523 sample Substances 0.000 description 23
- 238000001514 detection method Methods 0.000 description 12
- 238000003384 imaging method Methods 0.000 description 9
- 230000033001 locomotion Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001069 Raman spectroscopy Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 238000004458 analytical method Methods 0.000 description 1
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- 230000001427 coherent effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
- G02B21/0044—Scanning details, e.g. scanning stages moving apertures, e.g. Nipkow disks, rotating lens arrays
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Abstract
The invention discloses a rotary objective lens with an adjustable angle and a microscope, belonging to the technical field of microscopes and providing the following scheme: the rotary objective lens with the adjustable angle comprises an optical path relay frame, a dichroic mirror scanner and an objective lens; the optical path relay frame is used for transmitting the pulse laser to the dichroic mirror scanner; the dichroic mirror scanner is used for receiving the pulse laser transmitted by the optical path relay frame and transmitting the pulse laser to an objective lens of an external microscope; the device also comprises an optical filter and a focusing lens which are positioned on the same optical path with the dichroic mirror scanner and the objective lens, and the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens move integrally relative to the optical path relay frame. The technical scheme of the invention realizes the objective lens which integrally rotates in multiple angles by taking the front focus of the microscope objective lens as an axis, thereby greatly simplifying the scanning light path of the objective lens.
Description
Technical Field
The invention relates to the technical field of microscopes, in particular to a rotary objective lens with an adjustable angle and a microscope.
Background
The need for imaging living biological samples has prompted the rapid development of a variety of microscopic imaging techniques in recent years, with conventional microscopes, whether upright or inverted, typically having a fixed imaging optical path perpendicular to the horizontal plane, i.e., the plane in which the sample lies. However, in the case of imaging a living organism sample, the shape of the living organism has a specific three-dimensional shape, the surface is uneven, the imaging portion of the living organism is different, and the microscope needs to be continuously adjusted during normal operation. Therefore, the fixed imaging light path of the ordinary microscope is difficult to meet the requirement of living biological sample imaging.
In order to image a living biological sample, an optical path of a microscope objective lens capable of realizing multi-degree-of-freedom rotation by taking a focus as a center is required, and for a scanning microscope, if the optical path of the rotating optical path changes when the objective lens rotates, which means that the optical path between a sleeve lens and the objective lens changes, the optical path between a scanning lens and a scanning head needs to be adjusted correspondingly.
Disclosure of Invention
The invention mainly aims to provide an angle-adjustable rotary objective and a microscope, in particular to an objective which realizes multi-angle integral rotation by taking a front focus of the objective of the microscope as an axis, and greatly simplifies a scanning light path of the objective.
The basic scheme provided by the invention is as follows:
the rotary objective lens with the adjustable angle comprises an optical path relay frame, a dichroic mirror scanner and an objective lens;
the optical path relay frame is used for transmitting pulse laser to the dichroic mirror scanner;
the dichroic mirror scanner is used for receiving the pulse laser transmitted by the optical path relay frame and transmitting the pulse laser to an objective lens of an external microscope; wherein,
the optical filter and the focusing lens are positioned on the same optical path as the dichroic mirror scanner and the objective lens, and the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens move integrally relative to the optical path relay frame.
The principle of the basic scheme of the invention is as follows:
in the scheme, the transmission of pulse laser is realized through a laser, an optical path relay frame and a dichroic mirror scanner, the sample to be detected is irradiated through an objective lens, and after the sample to be detected is irradiated, reflected light is reflected to an external analytical instrument through an optical filter and a focusing lens. Meanwhile, the central axes of the objective lens, the dichroic mirror, the optical filter and the focusing lens are positioned on the same straight line, namely on a transmission line of the same optical path, and as a whole, the objective lens, the dichroic mirror, the optical filter and the focusing lens can move on an X-Y axis of a two-dimensional plane and up and down at multiple angles relative to the optical path relay frame so as to detect a sample to be detected. It should be noted that the angle-adjustable rotary objective lens of the present embodiment is suitable for various full field/scanning microscopes for imaging living biological samples.
The basic scheme has the beneficial effects that:
(1) in the scheme, the objective lens can realize multi-degree-of-freedom rotation by taking the focus of a front focal plane as an axis, and the objective lens, the dichroic mirror, the optical filter and the focusing lens move integrally relative to the multi-degree-of-freedom of the optical path relay frame, so that when a sample to be detected is detected, the position of the sample to be detected does not need to be adjusted, the objective lens does not need to be focused repeatedly, and other optical paths of the microscope do not need to be adjusted correspondingly;
(2) in the scheme, the objective lens, the dichroic mirror scanner, the optical filter and the focusing lens are positioned on the same light path, and the scanning lens and the focusing lens are reduced by the arranged dichroic mirror scanner, so that the scheme is simpler in structure, saves materials and reduces cost;
(3) in the scheme, through the arrangement of the dichroic mirror scanner, compared with the related technology, the arrangement of scanning lenses and focusing lenses is reduced, and the scanning light path of the objective lens is greatly simplified;
(4) in the scheme, the objective lens, the dichroic mirror, the optical filter and the focusing lens are integrally arranged on the same optical path, so that the optical filter is convenient to be matched with a full-field or scanning microscope for use.
Further, the dichroic mirror scanner is disposed between the optical filter and the objective lens to receive the pulsed laser transmitted by the optical path relay frame.
Through the position setting of dichroic mirror scanner, light filter and objective, simplified the microscope structure of this scheme, saved the material, the cost is reduced.
Further, the dichroic mirror scanner is an optical device with a scanning head and a dichroic mirror, which are integrally arranged.
By integrally providing the scanning head and the dichroic mirror as a dichroic mirror scanner, the scanning optical path of the objective lens is greatly simplified.
Further, the dichroic mirror scanner is a biaxial dichroic mirror scanner.
The multi-angle scanning is realized through the biaxial dichroic mirror scanner, so that the detection range of a sample to be detected is larger.
Further, the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens perform integral rotation of the X-Y axis with the front focus of the objective lens as an axis.
Through the integral rotation of the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens on the X-Y axis, the detection range of the objective lens of the microscope on a sample to be detected is enlarged, and the detection convenience of the objective lens on the sample to be detected is improved.
Further, the dichroic mirror scanner, the objective lens, the optical filter, and the focusing lens vertically move up and down in the vertical direction.
Through vertical motion of dichroic mirror scanner, objective, light filter and focusing lens in the vertical direction, promoted the detection scope of microscope's objective to waiting to detect the sample, promoted the detection convenience of objective to waiting to detect the sample.
Furthermore, a photomultiplier tube is arranged on the same straight line of the optical filter and the focusing lens.
Through the arrangement of the photomultiplier, the reflected light of the sample to be detected is received and converted, and the sample to be detected is better detected.
Further, the optical path relay frame comprises a plurality of relay lenses and a plurality of right-angle optical adjusting frames respectively connected between the relay lenses;
the relay lens is used for converting an angle scanning collimated light beam of a sleeve lens in an external microscope into a parallel focusing light beam;
and the right-angle optical adjusting frame is used for installing a reflector to reflect the light beam for 90 degrees.
Through the arrangement of the relay lens of the optical path relay frame and the right-angle optical adjusting frame, after laser emitted by the laser emitter is reflected to the dichroic mirror scanner, the optical path of the laser emitted by the dichroic mirror scanner can be kept unchanged when the objective lens rotates, and other optical paths of the microscope do not need to be correspondingly adjusted.
In order to achieve the above object, the present invention further provides a microscope including a laser and an optical path relay, the microscope including the angle-adjustable rotating objective lens as described above;
the angle-adjustable rotary objective comprises an optical path relay frame, a dichroic mirror scanner and an objective lens;
the optical path relay frame is used for transmitting pulse laser to the dichroic mirror scanner;
the dichroic mirror scanner is used for receiving the pulse laser transmitted by the optical path relay frame and transmitting the pulse laser to an objective lens of an external microscope; wherein,
the optical filter and the focusing lens are positioned on the same optical path as the dichroic mirror scanner and the objective lens, and the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens move integrally relative to the optical path relay frame.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of an adjustable-angle objective lens of the present invention;
FIG. 2 is a schematic diagram of a prior art arrangement of one embodiment of a rotating objective lens of a microscope;
fig. 3 is a schematic structural diagram of an embodiment of the entire rotation of the angle-adjustable rotary objective lens according to the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the overall rotating unit 10, the laser 20, the dichroic mirror scanner 30, the objective lens 40, the photomultiplier 50, the optical filter 60, the focusing lens 70, the relay lens 80, the right-angle optical adjustment frame 90, the scanner head 31, the scanning lens 32, the sleeve lens 33, and the dichroic mirror 34.
In one embodiment, referring to fig. 1, applied to a microscope having a laser 20 and an optical relay mount, the angularly adjustable rotating objective lens includes an optical relay mount, a dichroic mirror scanner 30, and an objective lens 40;
the optical path relay frame is configured to transmit the pulse laser to the dichroic mirror scanner 30;
the dichroic mirror scanner 30 is configured to receive the pulse laser transmitted through the optical path relay frame, and transmit the pulse laser to an objective lens 40 of an external microscope; wherein,
further, the optical path relay apparatus includes an optical filter 60 and a focusing lens 70 which are located in the same optical path as the dichroic mirror scanner 30 and the objective lens 40, and the dichroic mirror scanner 30, the objective lens 40, the optical filter 60, and the focusing lens 70 are integrally moved with respect to the optical path relay frame.
For the objective lens of the microscope in the related art as shown in fig. 2, the objective lens has the structure of the scanning lens 32, the sleeve lens 33 and the plurality of dichroic mirrors 34, when the sample to be detected needs to be detected at multiple angles, the transmitted pulse laser may be affected, the angle of the light path changes more, and other light paths of the microscope may need to be adjusted correspondingly; in the scheme, the transmission of the pulse laser is realized through the laser 20, the optical path relay frame and the dichroic mirror scanner 30, so that the sample to be detected is irradiated through the objective lens 40, and after the sample to be detected is irradiated, the reflected light is reflected to an external analysis instrument through the optical filter 60 and the focusing lens 70. When the multi-angle detection of a sample to be detected is needed, the dichroic mirror scanner 30, the objective lens 40, the optical filter 60 and the focusing lens 70 are integrally rotated as an integral rotating part 10; specifically, through the dichroic mirror scanner 30 that has the integrative setting of scanning head 31 and the optical device of dichroic mirror 34, scanning lens 32, sleeve lens 33 and dichroic mirror 34's structure has been reduced for the simple structure of this scheme has saved the material, and the cost is reduced has simplified the scanning light path when treating the sample detection that detects.
It should be noted that, in the conventional structure in the related art, a scanning lens and a sleeve lens with different focal lengths are added between the scanner and the objective lens, so that the conversion from a large angle of a thin beam at the scanner end to a small angle of a thick beam at the objective lens end is realized. Since in the development of microscopes, the microscope is an existing finite conjugate objective lens, the combination of an infinite conjugate objective lens and a telescopic lens ensues, facilitating the insertion of the elements of the filter in the parallel optical path between the two. The combination of the dichroic mirror and the scanner directly eliminates the scanning lens and the sleeve lens, and the beam diameter and angle of the scanner are completely equal to those of the objective lens, so that the objective lens needs to be redesigned. I.e. not compatible with all objectives of the conventional structure, whereas for multiphoton fluorescence scanning imaging only 1-2 objectives are needed in most experiments requiring the use of a microscope.
In addition, referring to fig. 2 and fig. 3, the installation positions of the objective lens 40, the dichroic mirror 34, the optical filter 60, and the focusing lens 70 are on a straight line transmitted by the same optical path, and the dichroic mirror scanner 30, the objective lens 40, the optical filter 60, and the focusing lens 70 perform an integral movement of the X-Y axis with the focal point of the front focal plane of the objective lens 40 as an axis, and perform a vertical movement in the vertical direction, that is, rotate the integral at multiple angles, to detect a sample to be detected. It is understood that the horizontal movement of the dichroic mirror scanner 30, the objective lens 40, the optical filter 60, and the focusing lens as a whole in the X-Y axis and the vertical movement up and down in the vertical direction are all the whole movements relative to the optical path relay frame, and when the optical path relay frame reflects the pulsed laser, since the optical paths of the relay lens 80 and the right-angle optical adjustment frame 90 in the optical path relay frame are overlapped, the reflected optical path is not affected when the whole moves relative to the optical path relay frame.
In one embodiment, referring to fig. 2, in the angle-adjustable rotary objective, a photomultiplier tube 50 is further disposed on the same line of the filter 60 and the focusing lens 70, the photomultiplier tube 50 converts a weak optical signal into an electrical signal, and the photomultiplier tube 50 is used in an optical measuring instrument and a spectroscopic analyzer, and can measure infinitesimal radiation power of 200 and 1200 nm in terms of low-level photometry and spectroscopy. The laser detection instrument uses a photomultiplier tube 50 as an effective receiver to receive the weak optical signal reflected by the sample to be detected.
In the related art, the scanning lens 32 of the microscope images the angle-scanning collimated light beam reflected by the scanning head 31 on the front focal plane thereof, and is converted into the angle-scanning collimated light beam by the sleeve lens 33 to converge on the rear focal point thereof, and then is imaged on the rear focal plane thereof by a relay lens 80, and finally is converted into the angle-scanning collimated light beam by another relay lens 80 to converge on the rear focal point thereof and enter the objective lens 40. In the scheme, pulse laser emitted by the laser 20 is directly irradiated to a sample to be detected through the dichroic mirror scanner 30 and the objective lens 40, and light emitted by the sample to be detected is directly reflected to the photomultiplier tube 50 through the focusing lens 70 and the optical filter 60 for processing; the structure of the scheme is simple, the material is saved, and the cost is reduced.
In the above embodiment, the optical path relay mount in the microscope includes the plurality of relay lenses 80 and the plurality of right-angle optical adjustment mounts 90 respectively connected between the relay lenses 80; a relay lens 80 for converting the angle-scanning collimated beam of the sleeve lens 33 in the external microscope into a parallel focused beam, wherein the relay lens 80 can be any lens or combination of lenses of any material, wavelength, shape and focal length according to the requirement; and a right-angle optical adjustment frame 90 for installing a reflector to rotate the light beam by 90 degrees, wherein the reflector can be made of any material, wavelength and shape according to requirements.
The relay lens 80 and the right-angle optical adjusting frame 90 in the above embodiments are cage-shaped cubic mechanical arms for transmitting pulse signals emitted by the laser 20, the cage-shaped cubic mechanical arms are used for accommodating different optical elements, when the angle-adjustable rotary objective lens of the present embodiment is used for NDD detection (common in nonlinear optical imaging such as multi-photon excited fluorescence, second/third harmonic generation, stimulated raman scattering, coherent anti-stokes raman scattering, etc.), a dichroic mirror 34 and an optical filter 60 are arranged in the cage-shaped cubic, one output end of the cage-shaped cubic is connected with an external objective lens 40 or an adapter thereof, the other output end is connected with an external photoelectric detection device or a light guide element, the output of the external photoelectric detector is connected with a microscope, the light guide element is connected with an original photoelectric detection device of the microscope, when the angle-adjustable rotary objective lens of the present embodiment is not used for NDD detection (common in a full-field microscope, a confocal scanning microscope, etc.), the reflector is arranged in the cage-shaped cube, and the output end of the cage-shaped cube is connected with the external objective lens 40 or an adapter thereof.
In order to achieve the above object, the present invention also proposes a microscope applied to a microscope having a laser 20 and an optical path relay stand, the microscope including an angle-adjustable rotating objective lens as described above;
the angle-adjustable rotary objective comprises an optical path relay frame, a dichroic mirror scanner and an objective lens;
the optical path relay frame is used for transmitting pulse laser to the dichroic mirror scanner;
the dichroic mirror scanner is used for receiving the pulse laser transmitted by the optical path relay frame and transmitting the pulse laser to an objective lens of an external microscope; wherein,
the optical filter and the focusing lens are positioned on the same optical path as the dichroic mirror scanner and the objective lens, and the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens move integrally relative to the optical path relay frame.
The specific structure of the microscope refers to the above embodiments, and since the microscope adopts all technical solutions of all embodiments of the above angle-adjustable rotating objective lens, at least all beneficial effects brought by the technical solutions of the above embodiments are achieved, and no further description is given here.
The foregoing are merely exemplary embodiments of the present invention, and no attempt is made to show structural details of the invention in more detail than is necessary for the fundamental understanding of the art, the description taken with the drawings making apparent to those skilled in the art how the several forms of the invention may be embodied in practice with the teachings of the invention. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (9)
1. The rotary objective lens with the adjustable angle is characterized by comprising an optical path relay frame, a dichroic mirror scanner and an objective lens;
the optical path relay frame is used for transmitting pulse laser to the dichroic mirror scanner;
the dichroic mirror scanner is used for receiving the pulse laser transmitted by the optical path relay frame and transmitting the pulse laser to an objective lens of an external microscope; wherein,
the optical filter and the focusing lens are positioned on the same optical path as the dichroic mirror scanner and the objective lens, and the dichroic mirror scanner, the objective lens, the optical filter and the focusing lens move integrally relative to the optical path relay frame.
2. The angle-adjustable rotary objective lens according to claim 1, wherein the dichroic mirror scanner is disposed between the optical filter and the objective lens to receive the pulsed laser light transmitted from the optical relay frame.
3. The angularly adjustable rotating objective lens of claim 2, characterized in that the dichroic mirror scanner is an integrated optics with a scanning head and a dichroic mirror.
4. Angle-adjustable rotating objective according to claim 3, characterized in that the dichroic mirror scanner is a biaxial dichroic mirror scanner.
5. The angle-adjustable rotating objective lens according to claim 1, wherein the dichroic mirror scanner, the objective lens, the optical filter, and the focusing lens perform an integral X-Y axis rotation about a front focal point of the objective lens.
6. The angularly adjustable rotating objective lens of claim 1, characterized in that the dichroic mirror scanner, objective lens, optical filter and focusing lens are vertically moved up and down in a vertical direction.
7. Angle-adjustable rotary objective according to claim 1, characterized in that a photomultiplier is arranged in line with the filter and the focusing lens.
8. The angle-adjustable rotary objective lens according to claim 1, wherein the optical path relay frame comprises a plurality of relay lenses and a plurality of right-angle optical adjustment frames respectively connected between the relay lenses;
the relay lens is used for converting an angle scanning collimated light beam of a sleeve lens in an external microscope into a parallel focusing light beam;
and the right-angle optical adjusting frame is used for installing a reflector to reflect the light beam for 90 degrees.
9. Microscope, characterized in that it comprises an angularly adjustable rotating objective as claimed in any of claims 1 to 8.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110654766.1A CN113359287A (en) | 2021-06-11 | 2021-06-11 | Angle-adjustable rotary objective and microscope |
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| CN202110654766.1A CN113359287A (en) | 2021-06-11 | 2021-06-11 | Angle-adjustable rotary objective and microscope |
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Cited By (1)
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Application publication date: 20210907 |