CN111529426A - Application of plant small molecules in improving skin elasticity and preparing beauty skin care products - Google Patents

Application of plant small molecules in improving skin elasticity and preparing beauty skin care products Download PDF

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CN111529426A
CN111529426A CN202010539044.7A CN202010539044A CN111529426A CN 111529426 A CN111529426 A CN 111529426A CN 202010539044 A CN202010539044 A CN 202010539044A CN 111529426 A CN111529426 A CN 111529426A
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skin
carbonyl
improving
dermal fibroblasts
acetyl alisol
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王曦
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Nanjing Aosaisi Biotechnology Co ltd
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Nanjing Aosaisi Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/63—Steroids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations

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  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses application of plant micromolecules in the aspects of improving skin elasticity and preparing beauty skin care products. Dermal fibroblasts are the most prominent cells in the dermis layer and constitute the main body of the dermis together with self-secreted collagen, elastin and other extracellular matrix components. Numerous studies have demonstrated that fibroblasts, due to their characteristic biological changes, play an important role in the process of skin aging. If the proliferation activity of dermal fibroblasts is reduced, the skin is slowly renewed, the repair capability is poor, and the skin is gradually aged; if the elastin content in dermal fibroblasts is reduced, a reduction in skin elasticity results. The research of the invention finds that the 16-carbonyl-24-acetyl alisol A can promote the proliferation of dermal fibroblasts, promote the renewal of skin and improve the skin repair capacity; increasing elastin content and improving skin elasticity.

Description

Application of plant small molecules in improving skin elasticity and preparing beauty skin care products
Technical Field
The invention belongs to the field of cosmetics, relates to improvement of skin elasticity, and particularly relates to application of plant small molecules in improvement of skin elasticity and preparation of a beauty skin care product.
Background
With age, various tissues and organs including the skin are gradually aged. Skin aging is mainly manifested by thinning, slow renewal, appearance of wrinkles, dryness and roughness, pigmentation, skin laxity, and reduced elasticity.
Dermal fibroblasts are the most prominent cells in the dermis layer and constitute the main body of the dermis together with self-secreted collagen, elastin and other extracellular matrix components. Numerous studies have demonstrated that fibroblasts, due to their characteristic biological changes, play an important role in the process of skin aging.
The concrete expression is as follows: the proliferation activity of dermal fibroblasts is reduced, so that the skin is slowly renewed, the skin repair capacity is poor, and the skin is gradually aged; a decrease in the amount of elastin in dermal fibroblasts, a decrease in skin elasticity; and so on.
Chendi and the like have studied the anti-aging effect of glacier water and snow tea extracts on normal human dermal fibroblasts and potential mechanisms thereof, and as a result, found that glacier water and snow tea extracts can improve the proliferation activity and energy metabolism level of human dermal fibroblasts, promote the expression effect of hyaluronic acid and elastin, and improve skin elasticity (experimental research on the delay of human dermal fibroblast aging by glacier water and snow tea extracts, natural product research and development, vol. 30 of 2018).
16-carbonyl-24-acetyl alisol A is a natural chemical component existing in alisma orientale, and no research on the aspect of improving skin elasticity is available.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the application of a plant micromolecule in improving the skin elasticity and preparing a beauty skin care product, wherein the plant micromolecule is 16-carbonyl-24-acetyl alisol A.
The purpose of the invention is realized by the following technical scheme:
an application of a plant small molecule in improving skin elasticity is provided, wherein the plant small molecule is 16-carbonyl-24-acetyl alisol A.
Application of 16-carbonyl-24-acetyl alisol A in improving skin repair capability.
A skin caring product for improving skin elasticity and skin repairing ability contains 16-carbonyl-24-acetyl alisol A.
Further, the beauty skin care product is facial cleanser, and the formula is as follows.
Figure BDA0002538210810000011
Figure BDA0002538210810000021
Further, the beauty skin care product is a face cream, and the formula is as follows.
Class of component Mass fraction of the components
Glycerol 9％
Butanediol 4.5％
Olive fruit oil 2％
1, 2-hexanediol 0.6％
Sorbitan fatty acid ester 0.8％
Aminomethyl propanol 0.5％
Hyaluronic acid sodium salt 0.08％
Xanthan gum 0.03％
Guar gum 0.03％
Essence 0.03％
16-carbonyl-24-acetyl alisol A 0.5％
Ethylenediaminetetraacetic acid disodium salt 0.05％
Deionized water Make up to 100%
Further, the beauty skin care product is a facial mask, and the formula is as follows.
Figure BDA0002538210810000022
Figure BDA0002538210810000031
Has the advantages that:
dermal fibroblasts are the most prominent cells in the dermis layer and constitute the main body of the dermis together with self-secreted collagen, elastin and other extracellular matrix components. Numerous studies have demonstrated that fibroblasts, due to their characteristic biological changes, play an important role in the process of skin aging. If the proliferation activity of dermal fibroblasts is reduced, the skin is slowly renewed, the repair capability is poor, and the skin is gradually aged; if the elastin content in dermal fibroblasts is reduced, a reduction in skin elasticity results. The research of the invention finds that the 16-carbonyl-24-acetyl alisol A can promote the proliferation of dermal fibroblasts, promote the renewal of skin and improve the skin repair capacity; increasing elastin content and improving skin elasticity.
Drawings
FIG. 1 shows the Western Blot results; the result shows that 16-carbonyl-24-acetyl alisol A with different concentrations can effectively improve the expression quantity of elastin in human dermal fibroblast HDF, and the effect of improving the expression quantity is more obvious when the concentration is higher.
Detailed Description
The following examples are intended to illustrate the essential technical content of the present invention, and the scope of the present invention should not be limited by the specific general details described in the following examples.
Example 1:
first, experimental material
Adult dermal fibroblasts HDF were purchased from ScienCell, usa.
Fetal bovine serum, DMEM medium was purchased from Gibco, USA.
The HPLC purity of the 16-carbonyl-24-acetyl alisol A is more than or equal to 98 percent.
MTT and DMSO were purchased from Sigma, USA.
Rabbit anti-human Elastin (Elastin) antibody, donkey anti-rabbit fluorescent secondary antibody were purchased from Abcam, UK.
Second, Experimental methods
1. Cell culture
Inoculating primary human dermal fibroblast HDF into DMEM medium containing 10% fetal calf serum, and culturing at 37 deg.C under 5% CO2Culturing under the condition of relative saturation humidity, carrying out passage once for 2-3 d, and taking the 5 th generation cell for experiment.
2. Determination of proliferation Activity of adult dermal fibroblasts
Taking human dermal fibroblast HDF in logarithmic growth phase, digesting, and re-suspending with DMEM medium containing 10% fetal calf serumCell concentration of 5 × 104A/mL cell suspension was seeded in 96-well plates at 100. mu.L per well. After the cells are completely attached to the wall, the drug group is replaced by DMEM medium containing 5 mu M, 10 mu M16-carbonyl-24-acetyl alisol A and 10% fetal calf serum for continuous culture, the control group is cultured by DMEM medium containing 10% fetal calf serum without drug, and culture solution without cells is arranged as a blank group at 37 ℃ and 5% CO2And continuously culturing for 24h under the relative saturation humidity condition, adding 20 mu L of 5mg/mL MTT solution into each hole, continuously culturing for 4h, absorbing and removing supernatant, adding 150 mu L of LDMSO into each hole, oscillating for 10min, measuring the light absorption value OD490 of each hole under the 490nm wavelength of an enzyme labeling instrument, and calculating the proliferation activity (%) of the human dermal fibroblast HDF of each drug group according to a formula by taking the proliferation activity of a control group as 100%. Each group of 5 multiple wells.
Proliferation activity (%) - (OD drug group-OD blank)/(OD control group-OD blank) × 100%
3. Determination of elastin expression levels in adult dermal fibroblasts
Taking human dermal fibroblast HDF in logarithmic growth phase, digesting, and suspending with DMEM medium containing 10% fetal calf serum to obtain cell concentration of 5 × 104Cell suspension/mL, seeded in 24-well plates at 400. mu.L per well. After the cells are completely attached to the wall, the drug group is replaced by DMEM medium containing 5 mu M, 10 mu M16-carbonyl-24-acetyl alisol A and 10% fetal calf serum for continuous culture, the control group is cultured by DMEM medium containing 10% fetal calf serum without drug, and the cells are cultured at 37 ℃ and 5% CO2And continuously culturing for 24 hours under the relative saturation humidity condition, collecting cells, washing, cracking, determining the protein concentration by using a BCA method, carrying out SDS-PAGE electrophoresis, transferring to a PVDF membrane, sealing by using skimmed milk powder, incubating overnight at 4 ℃ for resisting Elastin and β -actin, washing, adding a secondary antibody, incubating for 1 hour at room temperature, incubating and exposing by using a chemiluminescence developing reagent, and analyzing by using a chemiluminescence imaging system.
4. Data processing
Excel and SPSS 17.0 software are adopted, data are expressed by mean value +/-standard deviation, the difference between groups is compared by a t test, and the difference is considered to have significant difference and has statistical significance when P is less than 0.05.
Third, experimental results
1. Influence of 16-carbonyl-24-acetyl alisol A on human dermal fibroblast HDF proliferation activity
The influence of different concentrations of 16-carbonyl-24-acetyl alisol A on the proliferation activity of human dermal fibroblasts HDF is shown in Table 1, and the table 1 shows that the 16-carbonyl-24-acetyl alisol A with different concentrations can effectively promote the proliferation of the human dermal fibroblasts HDF, and the higher the concentration is, the better the proliferation promoting effect is.
TABLE 1 proliferative Activity of human dermal fibroblasts HDF for each drug group
Figure BDA0002538210810000051
2. Effect of 16-carbonyl-24-acetyl Alisol A on elastin expression in human dermal fibroblasts HDF
The influence of different concentrations of 16-carbonyl-24-acetyl alisol A on the expression of elastin in human dermal fibroblast HDF is shown in figure 1, and it can be found through figure 1 that different concentrations of 16-carbonyl-24-acetyl alisol A can effectively improve the expression level of elastin in human dermal fibroblast HDF, and the effect of improving the expression level is more obvious when the concentration is higher.
Dermal fibroblasts are the most prominent cells in the dermis layer and constitute the main body of the dermis together with self-secreted collagen, elastin and other extracellular matrix components. Numerous studies have demonstrated that fibroblasts, due to their characteristic biological changes, play an important role in the process of skin aging. If the proliferation activity of dermal fibroblasts is reduced, the skin is slowly renewed, the repair capability is poor, and the skin is gradually aged; if the elastin content in dermal fibroblasts is reduced, a reduction in skin elasticity results. The research of the invention finds that the 16-carbonyl-24-acetyl alisol A can promote the proliferation of dermal fibroblasts, promote the renewal of skin and improve the skin repair capacity; increasing elastin content and improving skin elasticity.
Example 2:
a facial cleanser for improving skin elasticity and skin repairing ability comprises the following formula:
class of component Mass fraction of the components
Myristic acid isopropyl ester 4％
Lanolin 3％
Sorbitan fatty acid ester 2.5％
Coconut diethanol amine 2.5％
Xanthan gum 0.08％
Coconut oil thickening foaming agent 0.02％
Phenoxyethanol 0.5％
Essence 0.03％
16-carbonyl-24-acetyl alisol A 0.3％
Deionized water Make up to 100%
Example 3:
a face cream for improving skin elasticity and skin repair capacity comprises the following formula:
class of component Mass fraction of the components
Glycerol 9％
Butanediol 4.5％
Olive fruit oil 2％
1, 2-hexanediol 0.6％
Sorbitan fatty acid ester 0.8％
Aminomethyl propanol 0.5％
Hyaluronic acid sodium salt 0.08％
Xanthan gum 0.03％
Guar gum 0.03％
Essence 0.03％
16-carbonyl-24-acetyl alisol A 0.5％
Ethylenediaminetetraacetic acid disodium salt 0.05％
Deionized water Make up to 100%
Example 4:
a facial mask for improving skin elasticity and skin repairing ability has the following formula:
Figure BDA0002538210810000061
Figure BDA0002538210810000071
the above-described embodiments are intended to embody the essential technical content of the present invention, and the scope of the present invention should not be limited by the specific general details described in the above-described embodiments.

Claims (6)

1. An application of a plant small molecule in improving skin elasticity is provided, wherein the plant small molecule is 16-carbonyl-24-acetyl alisol A.
2.16-carbonyl-24-acetyl alisol A is used for improving the skin repair capability.
3. A skin caring product for improving skin elasticity and skin repairing ability contains 16-carbonyl-24-acetyl alisol A.
4. The beauty skin care product according to claim 3, which is a facial cleanser and has the following formula.
Class of component Mass fraction of the components Myristic acid isopropyl ester 4％ Lanolin 3％ Sorbitan fatty acid ester 2.5％ Coconut diethanol amine 2.5％ Xanthan gum 0.08％ Coconut oil thickening foaming agent 0.02％ Phenoxyethanol 0.5％ Essence 0.03％ 16-carbonyl-24-acetyl alisol A 0.3％ Deionized water Make up to 100%
5. The beauty skin care product according to claim 3, which is a face cream and has the following formula.
Figure FDA0002538210800000011
Figure FDA0002538210800000021
6. The beauty skin care product according to claim 3, which is a mask and has the following formula.
Components Mass fraction Glycerol 9％ Konjak gum glucomannan 4.5％ Olive fruit oil 1.2％ Coconut diethanol amine 1.2％ Sorbitan fatty acid ester 1.5％ Xanthan gum 0.1％ Hyaluronic acid sodium salt 0.08％ Essence 0.02％ 16-carbonyl-24-acetyl alisol A 0.6％ Deionized water Make up to 100%
CN202010539044.7A 2020-06-13 2020-06-13 Application of plant small molecules in improving skin elasticity and preparing beauty skin care products Pending CN111529426A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130083778A (en) * 2012-01-13 2013-07-23 주식회사 엘지생활건강 Composition for improving skin wrinkle and enhancing elasticity
CN110314122A (en) * 2018-03-30 2019-10-11 深圳市前海博斐生物科技有限公司 A kind of Alisma extract and its preparation method and application

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130083778A (en) * 2012-01-13 2013-07-23 주식회사 엘지생활건강 Composition for improving skin wrinkle and enhancing elasticity
CN110314122A (en) * 2018-03-30 2019-10-11 深圳市前海博斐生物科技有限公司 A kind of Alisma extract and its preparation method and application

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
邰艳妮 等: "UPLC-MS/MS法同时测定泽泻药材中16 个成分", 《药物分析杂志》 *

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