CN110358736B - Modified K562 cells, preparation method thereof and NK cell culture composition - Google Patents
Modified K562 cells, preparation method thereof and NK cell culture composition Download PDFInfo
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- CN110358736B CN110358736B CN201910620164.7A CN201910620164A CN110358736B CN 110358736 B CN110358736 B CN 110358736B CN 201910620164 A CN201910620164 A CN 201910620164A CN 110358736 B CN110358736 B CN 110358736B
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
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Priority Applications (1)
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CN201910620164.7A CN110358736B (en) | 2016-05-20 | 2016-05-20 | Modified K562 cells, preparation method thereof and NK cell culture composition |
Applications Claiming Priority (2)
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CN201910620164.7A CN110358736B (en) | 2016-05-20 | 2016-05-20 | Modified K562 cells, preparation method thereof and NK cell culture composition |
CN201610340117.3A CN105838677B (en) | 2016-05-20 | 2016-05-20 | A kind of method and its application of efficient amplification freezen protective NK cell |
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CN201610340117.3A Division CN105838677B (en) | 2016-05-20 | 2016-05-20 | A kind of method and its application of efficient amplification freezen protective NK cell |
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CN110358736A CN110358736A (en) | 2019-10-22 |
CN110358736B true CN110358736B (en) | 2023-07-07 |
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CN201610340117.3A Active CN105838677B (en) | 2016-05-20 | 2016-05-20 | A kind of method and its application of efficient amplification freezen protective NK cell |
CN201910620164.7A Active CN110358736B (en) | 2016-05-20 | 2016-05-20 | Modified K562 cells, preparation method thereof and NK cell culture composition |
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Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2948462A1 (en) | 2014-05-15 | 2015-11-19 | National University Of Singapore | Modified natural killer cells and uses thereof |
CN107779432A (en) * | 2016-08-29 | 2018-03-09 | 灏灵赛奥(天津)生物科技有限公司 | CAR-T cell preparation and low-temperature storage medium |
CN117384929A (en) | 2017-03-27 | 2024-01-12 | 新加坡国立大学 | Polynucleotide encoding chimeric receptor expressed by cell |
RU2019128544A (en) * | 2017-03-27 | 2021-04-28 | Нэшнл Юниверсити Оф Сингапур | LINE OF STIMULATING CELLS FOR EX VIVO REPRODUCTION AND ACTIVATION OF NATURAL KILLER CELLS |
CN108034677A (en) * | 2017-12-28 | 2018-05-15 | 河南省华隆生物技术有限公司 | A kind of recombinant vector and its preparation method and application |
EP3773918A4 (en) | 2019-03-05 | 2022-01-05 | Nkarta, Inc. | Cd19-directed chimeric antigen receptors and uses thereof in immunotherapy |
CN111041048B (en) * | 2019-12-30 | 2021-07-06 | 北京鼎成肽源生物技术有限公司 | Preparation method of limited-generation trophoblast and culture method of SNK cells |
EP4163367A4 (en) * | 2020-06-09 | 2024-06-26 | Samsung Life Public Welfare Foundation | Genetically manipulated cell strain for activating and amplifying nk cells and use thereof |
WO2021251707A1 (en) * | 2020-06-09 | 2021-12-16 | 사회복지법인 삼성생명공익재단 | Genetically-modified cell line for nk cell activation and amplification, and use thereof |
CN112029721A (en) * | 2020-09-09 | 2020-12-04 | 广东昭泰体内生物医药科技有限公司 | Preparation method of activity-enhanced NK cells |
WO2022229884A2 (en) * | 2021-04-27 | 2022-11-03 | Takeda Pharmaceutical Company Limited | Recombinant antigen presenting cells |
Citations (4)
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CN101684456A (en) * | 2008-09-28 | 2010-03-31 | 江门罗森生物制药有限公司 | Method for amplifying NK cells of human beings under condition of in vitro culture |
CN102559600A (en) * | 2011-12-29 | 2012-07-11 | 上海交通大学医学院 | Artificial antigen presenting cell and application thereof in NK (natural killer) cell amplification |
CN105112370A (en) * | 2015-08-25 | 2015-12-02 | 深圳市科晖瑞生物医药有限公司 | Method for efficiently multiplying gamma delta T cells by stimulating peripheral blood in vitro and application of method |
CN105143441A (en) * | 2014-01-13 | 2015-12-09 | 深圳市汉科生物工程有限公司 | Method for preparing and using cell ghost with active factor as synergist of lymphocyte in vitro culture |
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2016
- 2016-05-20 CN CN201610340117.3A patent/CN105838677B/en active Active
- 2016-05-20 CN CN201910620164.7A patent/CN110358736B/en active Active
Patent Citations (4)
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CN101684456A (en) * | 2008-09-28 | 2010-03-31 | 江门罗森生物制药有限公司 | Method for amplifying NK cells of human beings under condition of in vitro culture |
CN102559600A (en) * | 2011-12-29 | 2012-07-11 | 上海交通大学医学院 | Artificial antigen presenting cell and application thereof in NK (natural killer) cell amplification |
CN105143441A (en) * | 2014-01-13 | 2015-12-09 | 深圳市汉科生物工程有限公司 | Method for preparing and using cell ghost with active factor as synergist of lymphocyte in vitro culture |
CN105112370A (en) * | 2015-08-25 | 2015-12-02 | 深圳市科晖瑞生物医药有限公司 | Method for efficiently multiplying gamma delta T cells by stimulating peripheral blood in vitro and application of method |
Non-Patent Citations (4)
Title |
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人NK细胞体外高效扩增的实验研究;黄庆生等;《细胞与分子免疫学杂志》;20081218(第12期);第39-41,45页 * |
体外扩增肾癌患者自体NK细胞及其对人肾细胞癌786-O细胞的杀伤;邵阳等;《中国肿瘤生物治疗杂志》;20121220(第06期);第81-85页 * |
双表达外源性4-1BBL/IL-15的K562细胞活化外周血淋巴细胞的研究;曹正锋等;《现代免疫学》;20070120(第01期);第14-19页 * |
细胞因子组合体外扩增人NK细胞的研究;周智锋等;《中华肿瘤防治杂志》;20140214(第03期);第43-47页 * |
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Publication number | Publication date |
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CN105838677A (en) | 2016-08-10 |
CN110358736A (en) | 2019-10-22 |
CN105838677B (en) | 2019-07-02 |
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