CN110346586A - Full-automatic detection apparatus - Google Patents
Full-automatic detection apparatus Download PDFInfo
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- CN110346586A CN110346586A CN201910760187.8A CN201910760187A CN110346586A CN 110346586 A CN110346586 A CN 110346586A CN 201910760187 A CN201910760187 A CN 201910760187A CN 110346586 A CN110346586 A CN 110346586A
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- reaction cup
- cup carrier
- driving part
- fluid storage
- carrier
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- 238000001514 detection method Methods 0.000 title claims abstract description 93
- 238000006243 chemical reaction Methods 0.000 claims abstract description 193
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- 239000012530 fluid Substances 0.000 claims abstract description 57
- 125000004122 cyclic group Chemical group 0.000 claims abstract description 13
- 238000010276 construction Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 abstract description 75
- 238000012360 testing method Methods 0.000 abstract description 21
- 230000007812 deficiency Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 27
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- 239000000758 substrate Substances 0.000 description 22
- 238000012546 transfer Methods 0.000 description 21
- 239000011324 bead Substances 0.000 description 18
- 238000003018 immunoassay Methods 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 9
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- 238000000034 method Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 3
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- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1011—Control of the position or alignment of the transfer device
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00564—Handling or washing solid phase elements, e.g. beads
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
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Abstract
This application involves the technical fields more particularly to full-automatic detection apparatus of medicine detection.The present apparatus includes: the top that reaction cup carrier is articulated with first support, and the first rotary driving part is connect with reaction cup carrier, to control the rotation of reaction cup carrier；Reaction cup carrier is equipped with reaction cup limit hole, so that reaction cup is fixed in reaction cup limit hole, the side wall of reaction cup carrier is equipped with through-hole, and through-hole and reaction cup correspond；The inside of opaque shell is arranged in optical sensor, and the side wall tight fit of opaque shell and reaction cup carrier, so that opaque shell and reaction cup carrier form enclosed construction, optical sensor is aligned by through-hole with reaction cup；The center of the cyclic structure of the reaction cup carrier is arranged in fluid storage cup load bearing component.The application can effectively solve detects that detection accuracy caused by a variety of testing liquids is low and the bulky technological deficiency of detection device using the same darkroom in the prior art.
Description
Technical field
This application involves the technical fields more particularly to full-automatic detection apparatus of medicine detection.
Background technique
It include various kinds of cell in blood sample, for example including leucocyte, red blood cell etc..By taking leucocyte as an example, and it is divided into
Property granulocyte, eosinophil, five class of basophilic granulocyte, monocyte and lymphocyte.
In recent years, keeping reforming with medical industry, quickly to detect POCT (real-time test, point-of-care
Testing) equipment is that the medical product of mark post is surging forward, and becomes an important symbol of medical industries reform and innovation.Currently,
Quickly detection is generally detected using optical detection, and optical detection detection is selected from chemiluminescence immunoassay technology, and chemiluminescence is exempted from
What epidemic disease analysis used has automatic chemiluminescence immunoassay system and semi-automatic chemiluminescence immunoassay system, full-automatic
Learn luminescence immunoassay system generally by reaction cup feed arrangement, sample pipetting volume device, reagent pipetting volume device, sample holding region,
Reagent storage area, incubation reaction device, Magneto separate cleaning device, apparatus for chemiluminescent detection and computer control system group
At, its main feature is that, entire chemiluminescence immune assay process is from reaction cup feeding, sample-adding, reagent adding, incubation, cleaning until changing
Learn shine measurement can realize automatic processing, without human intervention, have treating capacity it is big, it is time saving, rapidly and efficiently, automation journey
High advantage is spent, the large hospital big suitable for quantity of sample handling.
Existing apparatus for chemiluminescent detection is generally fixed to reaction cup survey, and testing principle is by the sample in reaction cup
Reaction solution is drawn onto the darkroom of apparatus for chemiluminescent detection, by detecting the chemiluminescence numerical value of sample reaction solution, obtains sample
The content of middle measured matter, but only one general darkroom of apparatus for chemiluminescent detection, when detecting multiple samples, darkroom needs to hold
Different sample reaction solutions is carried, causes the accuracy of chemiluminescence detection not high, it may appear that the case where false positive or false negative, together
When, due to needing, the realization sample transfer on a device, reaction solution is shifted, magnetic bead mixes, be incubated for, clean and detection substance contains
The operation of detection is measured, so that existing detection device needs many components to cause existing since these components are all separately provided
Detection device volume it is excessively huge.
Summary of the invention
This application provides full-automatic detection apparatus, effectively solve in the prior art using the same darkroom detect it is a variety of to
Survey caused by liquid that detection accuracy is low and the bulky technological deficiency of detection device.
In view of this, this application provides full-automatic detection apparatus, described device includes:
Reaction cup load bearing component, optical detection component and fluid storage cup load bearing component；
The reaction cup load bearing component includes the first support, reaction cup carrier and the first rotary driving part, described anti-
Glass carrier is answered to be articulated with the top of first support, first rotary driving part and the reaction cup carrier connect
It connects, to control the reaction cup carrier rotation；The reaction cup carrier is equipped with reaction cup limit hole, so that reaction cup is fixed
In the reaction cup limit hole, the side wall of the reaction cup carrier is equipped with through-hole, and the through-hole and the reaction cup are one by one
Corresponding, the reaction cup carrier is cyclic structure；
The optical detection component includes opaque shell and optical sensor, and the optical sensor is arranged described opaque
The side wall of the inside of shell, the opaque shell and the reaction cup carrier is fitted close, so that the opaque shell
Enclosed construction is formed with the reaction cup carrier, the optical sensor is aligned by the through-hole with the reaction cup；
The center of the cyclic structure of the reaction cup carrier is arranged in the fluid storage cup load bearing component；
The fluid storage cup load bearing component includes the second support, fluid storage cup carrier and the second rotary driving part
Part, the fluid storage cup carrier are articulated with the top of second support, second rotary driving part and the liquid
Body Storage Cup carrier connection, to control the fluid storage cup carrier rotation；The fluid storage cup carrier is equipped with liquid
Body Storage Cup limit hole, so that fluid storage cup is fixed in the fluid storage cup limit hole.
Preferably, first rotary driving part includes the first rotary drive motor and rotating platform；
First rotary drive motor and the rotating platform are rotatablely connected, to control the rotating platform rotation, institute
Rotating platform is stated to be fixedly connected with the reaction cup carrier.
Preferably, the reaction cup load bearing component further includes temperature control platform, the temperature control platform is fixed on
The bottom end of the reaction cup carrier.
Preferably, the optical detection component further includes pedestal, first direction driving part and second direction driving portion
Part, the first direction driving part is fixed on the base, and the second direction driving part and the first direction drive
Dynamic component connection, the first direction driving part controls the second direction driving part and moves in a first direction, described
Opaque shell is connect with the second direction driving part, and the second direction driving part controls the opaque shell and exists
Second party moves up.
Wherein, the optical sensor is existing conventional equipment, and optical sensor includes photodiode, photomultiplier tube
(PMT), the conventional equipments such as avalanche photodide (ADP) and photoelectric tube, connection relationship are also existing conventional connection relationship.
Preferably, the optical detection component further includes optical sensor mounting blocks, the opaque shell is mounted on institute
It states above optical sensor mounting blocks, so that the opaque shell and the optical sensor mounting blocks form optical sensor darkroom.
Preferably, the optical detection component further includes sample needle；One end of the sample needle passes through described opaque
The inside of the opaque shell is arranged in the roof of shell.
Preferably, the fluid storage cup carrier is cyclic structure or disc-shaped structure.
It should be noted that the full-automatic detection apparatus of the application can detecte chemiluminescent immunoreactant.
As can be seen from the above technical solutions, the application has the following advantages:
The application devises a kind of full-automatic detection apparatus, and testing liquid is built-in in reaction cup, and testing liquid is sample
With the mixture of chemiluminescence immunoassay reagent, reaction cup is placed in reaction cup limit hole, when the first rotary driving part drives
When reaction cup carrier rotates horizontally, the reaction cup on reaction cup carrier is rotated to the position being aligned with opaque shell,
At this point, the side wall of opaque shell and reaction cup carrier is fitted close, opaque shell and reaction cup carrier are formed and are closed
Structure, the testing liquid in reaction cup shine since chemiluminescence immunoassay has occurred, and the light source that reaction cup issues passes through through-hole
It is transferred to optical sensor, optical sensor detects light source, and obtains the information of test substance in testing liquid (such as by calculating analysis
Content etc.), and so on, continuous rotational response cup carrier is only needed, optical detection component and reaction cup carrier can form inspection
Darkroom is surveyed, can detecte the testing liquid in each reaction cup.As it can be seen that the application passes through to reaction cup carrier and optical detection
Component is transformed, and through-hole is arranged in reaction cup carrier, and optical detection component is provided with opaque shell, so that opaque shell
Enclosed construction is formed with reaction cup carrier, which is equivalent to the darkroom of optical detection, and optical sensor can be by logical
It is detected in the environment of enclosed construction and obtains the luminous value of testing liquid in hole.Therefore, the application no longer need to by reaction cup to
It surveys liquid to be delivered in optical detection component, can be detected the luminous value of each reaction cup, avoid in the prior art using same
Detect the technological deficiency of cross contamination caused by a variety of testing liquids in one darkroom.Meanwhile fluid storage cup load bearing component is arranged
In the hollow position of the cyclic structure of reaction cup carrier, the space of reaction cup carrier can be made full use of, reduces the application
Full-automatic detection apparatus area occupied, fluid storage cup carrier and reaction cup carrier are respectively revolved around same rotary shaft
Turn, the first rotary driving part and the second rotary driving part are independent driving part.Wherein, fluid storage cup can for for
The reaction solution or/and sample to be detected of chemiluminescence immune assay are stored, sample can be serum, blood, saliva, urine, abdomen
The liquid such as water or diffusate, fluid storage cup can be placed in fluid storage cup limit hole, facilitate reaction solution or/and to be detected
Sample is transferred in reaction cup.Fluid storage cup carrier is embedded in the hollow structure of reaction cup carrier, only needs one in this way
The transfer of solution and sample may be implemented in a liquid transfer needle, facilitates the transfer of liquid, and can effectively reduce the detection of the application
The volume of device.
Detailed description of the invention
Fig. 1 is the structure chart of full-automatic detection apparatus provided by the embodiments of the present application；
Fig. 2 is the structure chart of the reaction cup load bearing component of Fig. 1；
Fig. 3 is the structure chart of the optical detection component of Fig. 1；
Fig. 4 is another structure chart of the optical detection component of Fig. 1；
Fig. 5 is the structure chart of fluid storage cup load bearing component provided by the embodiments of the present application；
Fig. 6 is the top view of Fig. 1；
Fig. 7 is that the substrate of the application Fig. 6 cleans the structure chart of component；
Fig. 8 is the structure chart of the liquid transfering part of the application Fig. 6；
Fig. 9 is the structure chart of the reaction cup gripper components of the application Fig. 6；
Figure 10 is that the magnetic bead of the application Fig. 6 mixes the structure chart of component；
The liquid that Figure 11 is the application Fig. 6 shifts the structure chart that needle cleans component.
Specific embodiment
It is clearly and completely described below in conjunction with technical solution of the attached drawing to the embodiment of the present application, it is clear that described
Embodiment be the embodiment of the present application a part of the embodiment, instead of all the embodiments.Based on the reality in the embodiment of the present application
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the range of the embodiment of the present application protection.
In the description of the embodiment of the present application, it should be noted that term " center ", "upper", "lower", "left", "right",
The orientation or positional relationship of the instructions such as "vertical", "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings,
It is merely for convenience of description the embodiment of the present application and simplifies description, rather than the device or element of indication or suggestion meaning must have
There is specific orientation, be constructed and operated in a specific orientation, therefore should not be understood as the limitation to the embodiment of the present application.In addition,
Term " first ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the embodiment of the present application, it should be noted that unless otherwise clearly defined and limited, term " peace
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
The concrete meaning of language in the embodiment of the present application.
It should be understood that the application is applied to the system of medicine detection, Fig. 1 and Fig. 5 are please referred to, Fig. 1 mentions for the embodiment of the present application
The structure chart of the full-automatic detection apparatus of confession, Fig. 5 are the structure chart of fluid storage cup load bearing component provided by the embodiments of the present application,
As shown in Figure 1 and Figure 5, application embodiment includes reaction cup load bearing component 1, optical detection component 2 and fluid storage cup supporting part
Part 3；Reaction cup load bearing component 1 includes that the first support 1-1, reaction cup carrier 1-2 and the first rotary driving part, reaction cup are held
Load plate 1-2 is articulated with the top of the first support 1-1, and the first rotary driving part is connect with reaction cup carrier 1-2, anti-to control
Answer a glass carrier 1-2 rotation；Reaction cup carrier 1-2 is equipped with reaction cup limit hole 1-4, so that reaction cup is fixed on reaction cup limit
In the hole 1-4 of position, the side wall of reaction cup carrier 1-2 is equipped with through-hole 1-5, and through-hole 1-5 and reaction cup correspond；Optical detection portion
Part 2 includes opaque shell 2-1 and optical sensor, and optical sensor is arranged in the inside of opaque shell 2-1, opaque shell with
The side wall of reaction cup carrier 1-2 is fitted close, so that opaque shell 2-1 and reaction cup carrier 1-2 forms enclosed construction,
Optical sensor is aligned by through-hole 1-5 with reaction cup；The ring of reaction cup carrier 1-2 is arranged in fluid storage cup load bearing component 3
The center of shape structure；Fluid storage cup load bearing component 3 includes the second support, the rotation drive of fluid storage cup carrier 3-1 and second
Dynamic component 3-2, fluid storage cup carrier 3-1 are articulated with the top of the second support, and the second rotary driving part 3-2 and liquid store up
A glass carrier 3-1 connection is deposited, to control fluid storage cup carrier 3-1 rotation；Fluid storage cup carrier 3-1 is stored up equipped with liquid
A glass limit hole 3-3 is deposited, so that fluid storage cup is fixed in fluid storage cup limit hole 3-3.
The application devises full-automatic detection apparatus, and testing liquid is built-in in reaction cup, and testing liquid is sample and change
The mixture of electrochemiluminescent immunoassay reagent is learned, reaction cup is placed in reaction cup limit hole 1-4, when the driving of the first rotary driving part is anti-
When a glass carrier 1-2 being answered to rotate horizontally, the reaction cup on reaction cup carrier 1-2, which is rotated to opaque shell 2-1, to be aligned
Position, at this point, the side wall of opaque shell 2-1 and reaction cup carrier 1-2 are fitted close, opaque shell 2-1 with react
Cup carrier 1-2 forms enclosed construction, and the testing liquid in reaction cup shines since chemiluminescence immunoassay has occurred, reaction cup
The light source of sending is transferred to optical sensor by through-hole 1-5, and optical sensor detects light source, and obtains prepare liquid by calculating analysis
The information (such as content) of test substance in body, and so on, only need continuous rotational response cup carrier 1-2, optical detection portion
Part 2 can detecte the testing liquid in each reaction cup.As it can be seen that the application passes through to reaction cup carrier 1-2 and optical detection
Component 2 is transformed, and through-hole 1-5 is arranged in reaction cup carrier 1-2, and optical detection component 2 is provided with opaque shell 2-1, makes
It obtains opaque shell 2-1 and reaction cup carrier 1-2 and forms enclosed construction, which is equivalent to the darkroom of optical detection,
Optical sensor can be detected in the environment of enclosed construction by through-hole 1-5 and obtain the luminous value of testing liquid.Therefore, the application
It no longer needs to for the testing liquid in reaction cup being delivered in optical detection component, can be detected the luminous value of each reaction cup, keep away
The technological deficiency for detecting cross contamination caused by a variety of testing liquids using the same darkroom in the prior art is exempted from.Meanwhile liquid
The hollow position of the cyclic structure of reaction cup carrier 1-2 is arranged in body Storage Cup load bearing component 3, can make full use of reaction cup
The space of carrier 1-2, reduces the area occupied of the full-automatic detection apparatus of the application, fluid storage cup carrier 3-1 and anti-
Glass carrier 1-2 is answered respectively to rotate around same rotary shaft, the first rotary driving part 1-3 and the second rotary driving part 3-2
For independent driving part.Wherein, fluid storage cup can be for for storing the reaction solution of chemiluminescence immune assay or/and to be checked
Test sample sheet, sample can be placed on for liquid, fluid storage cups such as serum, blood, saliva, urine, ascites or diffusates
In fluid storage cup limit hole 3-3, reaction solution or/and sample to be detected is facilitated to be transferred in reaction cup.Therefore, fluid storage cup
Carrier 3-1 is embedded in the hollow structure of reaction cup carrier 1-2, and facilitating will be used in fluid storage cup carrier 3-1
The solution of detection is transferred in the reaction cup of reaction cup carrier 1-2, and can effectively reduce the volume of the application device.
Further, the second rotary driving part 3-2 conventional can be drivingly connected what object rotated to be existing
Equipment, the second rotary driving part 3-2 may include the second rotary drive motor and turntable；Second rotary drive motor and rotation
Turntable rotation connection, the second rotary drive motor to control turntable rotation, fix with fluid storage cup carrier 3-1 by turntable
Connection.Preferably, the second rotary drive motor can be servo motor.
Further, the fluid storage cup carrier 3-1 of the present embodiment is cyclic structure or disc-shaped structure.The liquid of Fig. 1
Body Storage Cup carrier 3-1 is disc-shaped structure, and the fluid storage cup carrier 3-1 of Fig. 5 is cyclic structure.
In order to make it easy to understand, referring to Fig. 2, Fig. 2 is the structure chart of the reaction cup load bearing component of Fig. 1, as shown in Fig. 2, instead
Answering glass carrier 1-2 is cyclic structure.Reaction cup limit hole 1-4 can be arranged in cyclic structure, through-hole 1-5 is arranged in ring
The outer wall of shape structure can efficiently use reaction cup carrier so that other devices can be set in the center of cyclic structure
Space.
Further, the first rotary driving part 1-3 can be the rotation of existing conventional achievable driving rotary motion
Driving device, in order to make it easy to understand, referring to Fig. 2, specifically, the first rotary driving part 1-3 of the present embodiment includes first
Rotary drive motor 1-6 and rotating platform 1-7；First rotary drive motor 1-6 and rotating platform 1-7 is rotatablely connected, the first rotation
Turn driving motor 1-6 to control rotating platform 1-7 rotation, rotating platform 1-7 is fixedly connected with reaction cup carrier 1-2.It is preferred that
, the first rotary drive motor 1-6 can be servo motor.
Further, the reaction cup load bearing component 1 of the present embodiment further includes temperature control platform 1-8, in order to make it easy to understand,
Referring to Fig. 2, temperature control platform 1-8 is fixed on the bottom end of reaction cup carrier 1-2.Temperature control platform 1-8 is for adjusting
The temperature of the reaction cup of reaction cup carrier 1-2, temperature control platform 1-8 can be existing conventional refrigeration and/or heating
Device, such as heater or refrigerator, the embodiment of the present application do not repeat specifically.Specifically, reaction cup load bearing component 1 can be with
Including support rod 1-9 and fixed block 1-10, temperature control platform 1-8 can connect by the way that support rod 1-9 and fixed block 1-10 is fixed
It connects, fixed block 1-10 is fixed on the surface of rotating platform 1-7, and the rotation of rotating platform 1-7 passes through fixed block 1-10 band dynamic temperature
Control platform 1-8 and reaction cup carrier 1-2 are rotated.
In order to make it easy to understand, referring to Fig. 3, Fig. 3 is the structure chart of the optical detection component of Fig. 1, as shown in figure 3, optics
Detection part 2 includes opaque shell 2-1 and optical sensor (not marking in figure), and optical detection component 2 is arranged in reaction cup
The shape of the side wall of carrier 1-2, opaque shell 2-1 can match with the shape of reaction cup carrier 1-2, opaque outer
Shell 2-1 can form shading cavity A, reaction cup carrier 1-2 can be in shading cavity A transfer by the way that anti-dazzling screen 2-2 is arranged
Dynamic, opaque shell 2-1 does not influence the rotation of reaction cup carrier 1-2, opaque shell 2-1 and reaction cup carrier 1-2's
Side wall is fitted close the enclosed construction to be formed, and can form the darkroom of optical detection component, optical sensor is by through-hole 1-5 and instead
Cup is answered to be aligned, so that optical sensor detects to obtain the luminous value of reaction cup by through-hole 1-5.
Further, the optical detection component 2 of the present embodiment further includes pedestal 2-3, first direction driving part 2-4 and
Two direction driving part 2-5, in order to make it easy to understand, referring to Fig. 4, Fig. 4 is another structure of the optical detection component of Fig. 1
Figure, first direction driving part 2-4 are fixed on pedestal 2-3, second direction driving part 2-5 and first direction driving part 2-
4 connections, first direction driving part 2-4 control second direction driving part 2-5 are moved on X in a first direction, opaque shell
2-1 is connect with second direction driving part 2-5, and second direction driving part 2-5 controls opaque shell 2-1 in second direction Y
Upper movement.First direction driving part 2-4 and second direction driving part 2-5 can drive opaque shell 2-1 in first party
It is moved on X and second direction Y, various sizes of reaction cup carrier 1-2 can be matched, so that optical detection component 2 can be with
Always it is fitted close with the side wall of reaction cup carrier 1-2, to effectively prevent endogenous smooth light leakage, and avoids by external light source
Interference, guarantees the accuracy of testing result.
Wherein, first direction driving part 2-4 can be the equipment that existing routine can make stretching motion, first in Fig. 4
Direction X is the radial direction of cricoid reaction cup carrier 1-2, and second direction Y is the axial direction of cricoid reaction cup carrier 1-2
Direction.First direction driving part 2-4 can be motor and screw structure, and second direction driving part 2-5 is fixed on first party
To on the nut of the lead screw of driving part 2-4, when the nut of the lead screw of first direction driving part 2-4 makees linear reciprocation,
Driving second direction driving part 2-5 also makees linear reciprocation；First direction driving part 2-4 may be motor, gear
With the structure of rack gear, motor is connect with gear, and wheel and rack is mediated, and second direction driving part 2-5 is fixed in a first direction
On the rack gear of driving part 2-4, when the rack gear of first direction driving part 2-4 makees linear reciprocation, driving second direction is driven
Dynamic component 2-5 also makees linear reciprocation.
Further, the optical sensor of the present embodiment is a kind of atomic weak light pulses theoretical based on direct detection quantum limit
Detection device, such as photon counter.The optical sensor of the present embodiment is existing conventional equipment, and optical sensor includes photoelectricity two
The conventional equipments such as pole pipe, photomultiplier tube (PMT), avalanche photodide (ADP) and photoelectric tube, connection relationship are also existing
Conventional connection relationship.
Further, referring to Fig. 4, the optical detection component 2 of the present embodiment further includes optical sensor mounting blocks 2-6, light
Sensor mounting blocks 2-6 is used for the installation of optical sensor, and opaque shell 2-1 is mounted on above optical sensor mounting blocks 2-6, makes
It obtains opaque shell 2-1 and optical sensor mounting blocks 2-6 and forms optical sensor darkroom.Optical detection component 2 can be by impermeable
Light shell 2-1 is connect with second direction driving part 2-5, and optical detection component 2 can also pass through photon counter mounting blocks 2-6
It is connect with second direction driving part 2-5.
Further, referring to Fig. 4, the optical detection component 2 of the present embodiment further includes sample needle 2-7；Sample needle 2-7's
The inside of opaque shell 2-1, the other end of sample needle 2-7 and the external world are arranged in across the roof of opaque shell 2-1 for one end
Feed container connection, sample needle 2-7 be used for reaction cup pump feed liquor body, sample needle 2-7 sample-adding liquid can for exciting liquid,
Enhancement solution etc. is used for the liquid of optical detection.
Further, referring to Fig. 6, Fig. 6 is the top view of Fig. 1, the full-automatic detection apparatus of the present embodiment further includes bottom
Object cleans component 4 and magnetic part, and the periphery of reaction cup carrier 1-2 is arranged in substrate cleaning component 4, and substrate cleans component 4
For cleaning the substrate in reaction cup, wherein the substrate of the application is the substance for participating in chemiluminescence immunoassay, specifically, to be measured
The measured matter and magnetic bead of sample specifically bind to form substrate to concentrate on the bottom of reaction cup, and substrate cleans component 4 and uses
In injection cleaning solution and the supernatant of absorption substrate, the substance not with magnetic bead specific binding is siphoned away, magnetic part setting
In the side of reaction cup carrier 1-2, magnetic part is used to for magnetic bead being adsorbed to the side of reaction cup.In order to make it easy to understand, Fig. 7
The structure chart of component is cleaned for the substrate of the application Fig. 5, it includes cleaning bracket 4-5, straight tube 4-3, curved tube 4- that substrate, which cleans component 4,
4, first direction driving part 4-1 and second direction driving part 4-2, wherein first direction X is cricoid reaction cup carrier
The radial direction of 1-2, second direction Y are the axial direction of cricoid reaction cup carrier 1-2；First direction driving part 4-1
It is connect with second direction driving part 4-2, first direction driving part 4-1 controls second direction driving part 4-2 in first party
It moves up, cleaning bracket 4-5 is connect with second direction driving part 4-2, second direction driving part 4-2 control cleaning bracket
4-5 is moved in a second direction, and straight tube 4-3 is fixed on cleaning bracket 4-5, and curved tube 4-4 is fixed on cleaning bracket 4-5, and
The bent of curved tube 4-4 controls cleaning branch to mouth towards straight tube 4-3, first direction driving part 4-1 and second direction driving part 4-2
The position of frame 4-5, so that the ullage of substrate of the straight tube 4-3 inside reaction cup, straight tube 4-4 injects cleaning solution, will react
Substrate in cup, which dilutes, to be mixed, and then the magnetic bead after cleaning is adsorbed onto the side of reaction cup by magnetic part, then, straight tube 4-3
It is inserted into the supernatant that substrate is drawn below the liquid level of the substrate of reaction cup, first direction driving part 4-1 and second party
The position that cleaning bracket 4-5 is controlled to driving part 4-2, so that straight tube 4-3 rises on the liquid level of the substrate inside reaction cup
Side, curved tube 4-4 can be conveyed in cleaning solution to reaction cup, while the bent of curved tube 4-4 is used for mouth towards straight tube 4-3 to straight tube 4-
The 3 liquid level positions for being inserted into the substrate inside reaction cup are cleaned, and repeat above procedure 2-4 times so as to inside to reaction cup
Substrate cleans up.Substrate, which cleans component 4, to be the existing conventional component for substrate cleaning.
Further, the full-automatic detection apparatus of the present embodiment further includes liquid transfering part 5, in order to make it easy to understand, Fig. 8
For the structure chart of the liquid transfering part of the application Fig. 5, liquid transfering part 5 can drive rotation and be stretched for existing routine
Equipment, the embodiment of the present application provides specific liquid transfering part 5, and liquid transfering part 5 is arranged in reaction cup carrier
The periphery of 1-2, liquid of the liquid transfering part 5 for fluid storage cup are transferred in the reaction cup of reaction cup carrier 1-2, liquid
Body Storage Cup can be placed on fluid storage cup carrier 3-1, and fluid storage cup can also be placed on liquid transfering part 5
Periphery；Liquid transfering part 5 includes swivel link 5-1, liquid transfer needle 5-2, liquid pump, second direction driving part and rotation
Direction driving part, liquid transfer needle 5-2 are connect with liquid pump, and liquid pump draws liquid and release for liquid transfer needle 5-2
Liquid, liquid transfer needle 5-2 are fixed on swivel link 5-1, and swivel link 5-1 is connect with second direction driving part, and second
Direction driving part drives swivel link 5-1 to make linear reciprocating movement in second direction, and swivel link 5-1 and direction of rotation drive
Component connection, direction of rotation driving part drive swivel link 5-1 around the rotation axis rotation of Z-direction driving part, rotation
Turning direction is Z-direction, and second direction Y is the axial direction of cricoid reaction cup carrier 1-2.
Further, the full-automatic detection apparatus of the present embodiment further includes reaction cup gripper components 6, in order to make it easy to understand,
Fig. 9 is the structure chart of the reaction cup gripper components of the application Fig. 5, and reaction cup gripper components 6 can drive for existing routine and grab
Taking hand, X and second direction Y makees linear reciprocal movement in the equipment with crawl in a first direction, and the embodiment of the present application provides specifically
Reaction cup gripper components 6, the periphery of reaction cup carrier 1-2, reaction cup gripper components 6 are arranged in reaction cup gripper components 6
For crawl reaction cup detach reaction cup carrier 1-2.Reaction cup gripper components 6 include first direction driving part 6-1, the
Two direction driving part 6-2 are connect with crawl wall 6-3, first direction driving part 6-1 with second direction driving part 6-2, the
X makees linear reciprocal movement to one direction driving part 6-1 driving second direction driving part 6-2 in a first direction, crawl wall 6-3 with
The 6-2 connection of second direction driving part, second direction driving part 6-2 driving crawl wall 6-3 make linear reciprocal in second direction Y
Movement, crawl wall 6-3 are the manipulator for grabbing reaction cup.
Further, the full-automatic detection apparatus of the present embodiment further includes that magnetic bead mixes component 7, in order to make it easy to understand, figure
10 mix the structure chart of component for the magnetic bead of the application Fig. 5, and it can be existing conventional for mixing magnetic bead that magnetic bead, which mixes component 7,
Even equipment, the embodiment of the present application do not repeat specifically.
Further, the full-automatic detection apparatus of the present embodiment further includes liquid transfer needle cleaning component 8, for the ease of reason
Solution, the liquid that Figure 11 is the application Fig. 5 shift the structure chart that needle cleans component, and it can be existing that liquid, which shifts needle cleaning component 8,
The conventional equipment for cleaning liquid transfer needle, such as Vltrasonic device, liquid transfer needle 5-3 can inject Vltrasonic device
In cleaning solution, needle 5-2 is shifted to liquid by ultrasonic vibration and is cleaned, the embodiment of the present application does not repeat specifically.
It should be noted that the first direction driving part of above embodiments can be motor and screw structure, second party
To on the fixed nut of the lead screw of driving part in a first direction of driving part, the nut of the lead screw of first direction driving part is made
When linear reciprocation, driving second direction driving part also makees linear reciprocation；First direction driving part may be
The structure of motor, gear and rack gear, motor are connect with gear, and wheel and rack is mediated, and second direction driving part is fixed on the
On the rack gear of one direction driving part, when the rack gear of first direction driving part makees linear reciprocation, driving second direction is driven
Dynamic component 2-5 also makees linear reciprocation.
Wherein, the application can be set a reaction cup load bearing component (can be disk), and around disk setting
One liquid transfer needle, a liquid transfer needle cleaning component, a magnetic bead mixes component, a substrate cleans component, one
Optical detection component and a reaction cup gripper components, with simplest structure arrangement, most saving space and simplest fortune
It is dynamic to complete automatic chemiluminescence immunoassay detection.And existing full-automatic detection apparatus is all multiple disk settings mostly,
Lead to that detection device volume is huge, structure is complicated.
It can be set it should be noted that magnetic bead mixes component 7 and liquid transfer needle cleaning component 8 in reaction cup carrier
The hollow position in cricoid fluid storage cup carrier 3-1 also can be set in the periphery of 1-2.
It should be noted that centered on reaction cup carrier 1-2, substrate cleans component 4, optical detection component 2 and anti-
Answer glass gripper components 6 in a counterclockwise direction or be successively set on the periphery of reaction cup carrier 1-2 clockwise, and with react
Cup carrier 1-2 is fitted close, to realize the operation to reaction cup inner material.
It should be noted that the device of the application realizes that automatic chemiluminescence immunoassay detects according to the following steps,
Include:
S101: reaction cup is placed in reaction cup limit hole 1-4, and fluid storage cup is placed on fluid storage cup limit hole
In 3-3, fluid storage cup divides into reagent cup and sample cup, and reagent needed for chemiluminescence immunoassay is put into reagent cup, sample
It is placed in sample cup, specific magnetic bead is placed on magnetic bead and mixes in component 7, and starting magnetic bead mixes 7 pairs of component specific magnetic beads
It is mixed；
S102: the sample of sample cup is transferred in reaction cup by start liquid transfering part 5, then shifts needle 5- to liquid
2 be transferred to liquid transfer needle cleaning component 8 in cleaned；Start liquid transfering part 5 is by the agent transfer of reagent cup to anti-
It answers in cup, then liquid transfer needle 5-2 is transferred in liquid transfer needle cleaning component 8 and is cleaned；Start liquid transfer portion
The specific magnetic bead that magnetic bead mixes component 7 is transferred in reaction cup by part 5, is then transferred to liquid to liquid transfer needle 5-2 and is turned
It moves in needle cleaning component 8 and is cleaned；
S103: drive response cup carrier 1-2 is rotated horizontally, and reaction cup is rotated to the cleaning of substrate cleaning component 4
Position；
S104: drive response cup carrier 1-2 is rotated horizontally, and reaction cup is rotated to the optics of optical detection component 2
Position is detected, reaction cup can be previously implanted luminous exciting liquid, can also add in optical detection component 2 by sample needle 2-7
Enter luminous exciting liquid, optical detection component 2 acquires the signal of reaction cup；
S105: drive response cup carrier 1-2 is rotated horizontally, and reaction cup is rotated to reaction cup gripper components 6, crawl
Wall 6-3 grabs reaction cup, and reaction cup is moved in reaction cup collection box, terminates.
The description of the present application and term " first " in above-mentioned attached drawing, " second ", " third ", " the 4th " etc. are (if deposited
) it is to be used to distinguish similar objects, without being used to describe a particular order or precedence order.It should be understood that use in this way
Data are interchangeable under appropriate circumstances, so that embodiments herein described herein for example can be in addition to illustrating herein
Or the sequence other than those of description is implemented.In addition, term " includes " and " having " and their any deformation, it is intended that
Cover it is non-exclusive include, for example, containing the process, method, system, product or equipment of a series of steps or units need not limit
In step or unit those of is clearly listed, but may include be not clearly listed or for these process, methods, produce
The other step or units of product or equipment inherently.
It should be appreciated that in this application, " at least one (item) " refers to one or more, and " multiple " refer to two or two
More than a."and/or" indicates may exist three kinds of relationships, for example, " A and/or B " for describing the incidence relation of affiliated partner
It can indicate: only exist A, only exist B and exist simultaneously tri- kinds of situations of A and B, wherein A, B can be odd number or plural number.Word
Symbol "/" typicallys represent the relationship that forward-backward correlation object is a kind of "or"." at least one of following (a) " or its similar expression, refers to
Any combination in these, any combination including individual event (a) or complex item (a).At least one of for example, in a, b or c
(a) can indicate: a, b, c, " a and b ", " a and c ", " b and c ", or " a and b and c ", and wherein a, b, c can be individually, can also
To be multiple.
The above, above embodiments are only to illustrate the technical solution of the application, rather than its limitations；Although referring to before
Embodiment is stated the application is described in detail, those skilled in the art should understand that: it still can be to preceding
Technical solution documented by each embodiment is stated to modify or equivalent replacement of some of the technical features；And these
It modifies or replaces, the spirit and scope of each embodiment technical solution of the application that it does not separate the essence of the corresponding technical solution.
Claims (8)
1. full-automatic detection apparatus characterized by comprising
Reaction cup load bearing component, optical detection component and fluid storage cup load bearing component；
The reaction cup load bearing component includes the first support, reaction cup carrier and the first rotary driving part, the reaction cup
Carrier is articulated with the top of first support, and first rotary driving part is connect with the reaction cup carrier, with
Control the reaction cup carrier rotation；The reaction cup carrier is equipped with reaction cup limit hole, so that reaction cup is fixed on institute
It states in reaction cup limit hole, the side wall of the reaction cup carrier is equipped with through-hole, and the through-hole and the reaction cup correspond,
The reaction cup carrier is cyclic structure；
The optical detection component includes opaque shell and optical sensor, and the optical sensor is arranged in the opaque shell
Inside, the side wall of the opaque shell and the reaction cup carrier is fitted close, so that the opaque shell and institute
It states reaction cup carrier and forms enclosed construction, the optical sensor is aligned by the through-hole with the reaction cup；
The center of the cyclic structure of the reaction cup carrier is arranged in the fluid storage cup load bearing component；
The fluid storage cup load bearing component includes the second support, fluid storage cup carrier and the second rotary driving part, institute
State the top that fluid storage cup carrier is articulated with second support, second rotary driving part and the fluid storage
Cup carrier connection, to control the fluid storage cup carrier rotation；The fluid storage cup carrier is equipped with fluid storage
Cup limit hole, so that fluid storage cup is fixed in the fluid storage cup limit hole.
2. full-automatic detection apparatus according to claim 1, which is characterized in that first rotary driving part includes the
One rotary drive motor and rotating platform；
First rotary drive motor and the rotating platform are rotatablely connected, to control the rotating platform rotation, the rotation
Turn platform to be fixedly connected with the reaction cup carrier.
3. full-automatic detection apparatus according to claim 1, which is characterized in that the reaction cup load bearing component further includes temperature
Control platform is spent, the temperature control platform is fixed on the bottom end of the reaction cup carrier.
4. full-automatic detection apparatus according to claim 1, which is characterized in that the optical detection component further includes bottom
Seat, first direction driving part and second direction driving part, the first direction driving part is fixed on the base, institute
It states second direction driving part to connect with the first direction driving part, the first direction driving part control described second
Direction driving part moves in a first direction, and the opaque shell is connect with the second direction driving part, and described
Two direction driving parts control the opaque shell and move in a second direction.
5. full-automatic detection apparatus according to claim 1, which is characterized in that the optical detection component further includes that light passes
Sensor mounting blocks, the opaque shell is mounted on above the optical sensor mounting blocks, so that the opaque shell and institute
It states optical sensor mounting blocks and forms optical sensor darkroom.
6. full-automatic detection apparatus according to claim 1, which is characterized in that the optical detection component further includes sample-adding
Needle；The inside of the opaque shell is arranged in the roof that one end of the sample needle passes through the opaque shell.
7. full-automatic detection apparatus according to claim 1, which is characterized in that the fluid storage cup carrier is disk
Shape structure.
8. full-automatic detection apparatus according to claim 1, which is characterized in that the fluid storage cup carrier is ring-type
Structure.
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| CN201910760187.8A CN110346586A (en) | 2019-08-16 | 2019-08-16 | Full-automatic detection apparatus |
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| CN201910760187.8A CN110346586A (en) | 2019-08-16 | 2019-08-16 | Full-automatic detection apparatus |
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| CN114406569A (en) * | 2022-01-21 | 2022-04-29 | 浙江盛越电子科技有限公司 | Full-automatic welding machine for coreless motor |
| CN115656493A (en) * | 2022-12-29 | 2023-01-31 | 山东康华生物医疗科技股份有限公司 | Independent circular darkroom automatic checkout mechanism |
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Application publication date: 20191018 |