CN110275011A - The sensitization detection method of colloidal gold immunity chromatography and application - Google Patents
The sensitization detection method of colloidal gold immunity chromatography and application Download PDFInfo
- Publication number
- CN110275011A CN110275011A CN201910386444.6A CN201910386444A CN110275011A CN 110275011 A CN110275011 A CN 110275011A CN 201910386444 A CN201910386444 A CN 201910386444A CN 110275011 A CN110275011 A CN 110275011A
- Authority
- CN
- China
- Prior art keywords
- colloidal gold
- sample
- detection
- preset
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 206010070834 Sensitisation Diseases 0.000 title claims abstract description 23
- 230000008313 sensitization Effects 0.000 title claims abstract description 23
- 238000004587 chromatography analysis Methods 0.000 title abstract 3
- 230000036039 immunity Effects 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 30
- 238000012360 testing method Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 28
- 238000003317 immunochromatography Methods 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 24
- 230000001235 sensitizing effect Effects 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical group Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 229910052737 gold Inorganic materials 0.000 claims description 4
- 239000010931 gold Substances 0.000 claims description 4
- 239000001509 sodium citrate Substances 0.000 claims description 4
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 4
- 229940038773 trisodium citrate Drugs 0.000 claims description 4
- 229960005070 ascorbic acid Drugs 0.000 claims description 3
- 235000010323 ascorbic acid Nutrition 0.000 claims description 3
- 239000011668 ascorbic acid Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 229920002521 macromolecule Polymers 0.000 claims description 3
- 230000009467 reduction Effects 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 10
- 238000011161 development Methods 0.000 abstract description 7
- 208000002109 Argyria Diseases 0.000 abstract description 4
- 239000000273 veterinary drug Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007447 staining method Methods 0.000 description 3
- 230000006957 competitive inhibition Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003263 anabolic agent Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000507 anthelmentic effect Effects 0.000 description 1
- 230000001165 anti-coccidial effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000000842 anti-protozoal effect Effects 0.000 description 1
- 239000006030 antibiotic growth promoter Substances 0.000 description 1
- 239000003904 antiprotozoal agent Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000009881 electrostatic interaction Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The embodiment of the invention provides a kind of sensitization detection method of colloidal gold immunity chromatography and applications, include: acquisition sample to be tested, and the first preset duration will be reacted in the well of the test sample of the first preset vol instillation colloidal gold immunochromatographykit kit；The enhanced sensitivity reagent that the second preset vol is instilled in the well reacts the second preset duration；It repeats the above steps repeatedly, until the reading variation of the colloidal gold immunochromatographykit kit completes the detection to the sample to be tested within preset range.By the way that enhanced sensitivity reagent is added during conventional colloidal gold immunity chromatography detects sample to be tested, the sensitivity of colloidal gold immunochromatographykit kit detection large biological molecule can be significantly improved, and colour developing is rapid, background interference is smaller than conventional silver staining, the prospect that is widely used and Development volue.
Description
Technical Field
The embodiment of the invention relates to the technical field of biological detection, and particularly relates to a sensitivity-enhancing detection method of a colloidal gold immunochromatography and application thereof.
Background
Veterinary drugs which are important in the residual toxicology significance mainly comprise antimicrobial drugs, anthelmintic drugs, anticoccidial and antiprotozoal drugs, antibiotic growth promoters, anabolic hormone growth promoters and the like according to the application. At present, the international universal veterinary drug residue detection method is to firstly adopt a simple method to carry out rapid primary screening on a sample to be detected, and then adopt a method with higher accuracy to carry out confirmation analysis on the sample which is initially screened to be positive. The problem of pollution of domestic foods by veterinary drugs is serious, a large amount of investment is made by the nation and the government, but the execution situation is still unsatisfactory, and one of the main reasons for the phenomenon is lack of a rapid, sensitive and simple detection method. Thin layer chromatography, ELISA, high performance liquid chromatography and mass spectrometry detection technologies, and the method needs instrument equipment to finish detection for a long time and is not suitable for on-site rapid detection. In recent years, the colloidal gold immunochromatography has the characteristics that the operation is simple, auxiliary instruments and reagents are not needed, the result is obtained in 3-5 minutes, and the judgment can be carried out by naked eyes. Therefore, the method is researched and applied to the field and primary screening inspection of large-batch veterinary drug residues.
Colloidal gold (gold) is an aqueous solution of chloroauric acid (chloroauric acid), which is polymerized into gold particles of a specific size under the action of a reducing agent such as white phosphorus, trisodium citrate, etc., and becomes a stable colloidal solution due to electrostatic interaction. The colloidal gold particles have the characteristic of high electron density, and when the particles are aggregated to reach a certain density, pink spots which are visible to the naked eye appear, so the colloidal gold particles can be used as indicators of immunochromatography tests. Therefore, the colloidal gold immunochromatography is a method using colloid (red) as a tracer. The immune labeling method is a novel immune labeling method which combines the immune labeling with various macromolecular substances such as protein and the like and then utilizes the antigen-antibody reaction to achieve the detection purpose. The GICA mainly includes a sandwich method and a competitive inhibition method, the sandwich method includes a double antibody sandwich method for detecting an antigen and a double antigen method for detecting an antibody, and the method is mainly used for detecting viruses, bacteria, parasites and the like. The competitive inhibition method is mainly used for detecting veterinary drug residues, steroids and pesticide small molecules (antigens).
However, the current colloidal gold immunochromatography has low sensitivity to macromolecules, and the popularization and application of the colloidal gold immunochromatography are limited.
Disclosure of Invention
Embodiments of the present invention provide a sensitivity-enhanced detection method of a gold immunochromatography method and an application thereof, which overcome the above problems or at least partially solve the above problems.
On one hand, the embodiment of the invention provides a sensitivity enhancing detection method of a colloidal gold immunochromatography, which comprises the following steps:
obtaining a sample to be detected, and dripping a first preset volume of the sample to be detected into a sample adding hole of the colloidal gold immunochromatography kit for reaction for a first preset time;
dripping a sensitization reagent with a second preset volume into the sample adding hole to react for a second preset time;
and repeating the steps for multiple times until the reading change of the colloidal gold immunochromatographic kit is within a preset range, thereby completing the detection of the sample to be detected.
Further, before the dropping the first preset volume of the detection sample into the loading hole of the colloidal gold immunochromatographic kit, the method further comprises:
and carrying out conventional pretreatment on the detection sample.
Further, the sensitizing reagent comprises a first sensitizing reagent, a second sensitizing reagent and a third sensitizing reagent which are mixed according to a preset volume ratio; wherein,
the first sensitization reagent is a chloroauric acid solution with the weight percentage concentration of 2% -5%, the second sensitization reagent is a reduction solution, and the third sensitization reagent is a comparison solution.
Further, the first sensitization reagent is a chloroauric acid solution with the weight percentage concentration of 3%.
Further, the second sensitization reagent is hydroxylamine hydrochloride solution with the molar concentration of 2-5% or ascorbic acid solution with the weight percentage concentration of 0.3%.
Further, the third sensitizing agent is trisodium citrate aqueous solution with the weight percentage concentration of 1%.
On the other hand, the embodiment of the invention provides the application of the method in biomacromolecule detection.
According to the sensitization detection method and the application of the colloidal gold immunochromatography provided by the embodiment of the invention, the sensitization reagent is added in the process of detecting the sample to be detected by the conventional colloidal gold immunochromatography, so that the sensitivity of the colloidal gold immunochromatography kit for detecting the biomacromolecule can be obviously improved, the color development is rapid, the background interference is less than that of the conventional silver staining method, and the method has a very wide application prospect and a very wide development value.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides a sensitivity enhancing detection method of a colloidal gold immunochromatography, which comprises the following steps:
step 1, obtaining a sample to be detected, and dripping a first preset volume of the sample to be detected into a sample adding hole of a colloidal gold immunochromatography kit for reaction for a first preset time;
step 2, dripping a sensitization reagent with a second preset volume into the sample adding hole to react for a second preset time;
and 3, repeating the steps for multiple times until the reading change of the colloidal gold immunochromatography kit is within a preset range, and finishing the detection of the sample to be detected.
Specifically, samples to be detected, such as animal serum, visceral extract, and the like, are respectively obtained as samples to be detected, depending on the objects to be detected. The colloidal gold immunochromatographic kit may be a conventional colloidal gold immunochromatographic kit commercially available.
The first preset time and the second preset time can be set according to the specific type of the object to be detected. For example, the first time period may be set to 1-10 minutes and the second time period may be set to 5-8 minutes.
The measurement of a sample to be measured can generally be satisfied by repeating the steps 1 to 2 two to three times.
According to the sensitization detection method of the colloidal gold immunochromatography provided by the embodiment of the invention, the sensitization reagent is added in the process of detecting the sample to be detected by the conventional colloidal gold immunochromatography, so that the sensitivity of the colloidal gold immunochromatography kit for detecting the biomacromolecule can be obviously improved, the color development is rapid, the background interference is less than that of the conventional silver staining method, and the method has a very wide application prospect and a very wide development value.
In the above embodiment, before the dropping the first preset volume of the detection sample into the loading hole of the colloidal gold immunochromatographic kit, the method further comprises:
and carrying out conventional pretreatment on the detection sample.
Specifically, the interference of impurities can be eliminated by carrying out conventional pretreatment on a detection sample, so that the detection result is more accurate.
In the above embodiment, the sensitizing reagent comprises a first sensitizing reagent, a second sensitizing reagent and a third sensitizing reagent which are mixed according to a preset volume ratio; wherein,
the first sensitization reagent is a chloroauric acid solution with the weight percentage concentration of 2% -5%, the second sensitization reagent is a reduction solution, and the third sensitization reagent is a comparison solution.
In the above embodiment, the first sensitizing reagent is a chloroauric acid solution with a concentration of 3% by weight.
In the above embodiment, the second sensitizing reagent is hydroxylamine hydrochloride solution with a molar concentration of 2% to 5% or ascorbic acid solution with a weight percentage concentration of 0.3%.
In the above examples, the third sensitising agent was an aqueous solution of trisodium citrate at a concentration of 1% by weight.
The embodiment of the invention also provides an application of the method in biomacromolecule detection.
According to the application of the sensitization detection method of the colloidal gold immunochromatography provided by the embodiment of the invention, the sensitization reagent is added in the process of detecting the sample to be detected by the conventional colloidal gold immunochromatography, so that the sensitivity of the colloidal gold immunochromatography kit for detecting the biomacromolecule can be obviously improved, the color development is rapid, the background interference is less than that of the conventional silver staining method, and the application prospect and the development value are very wide.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (7)
1. A sensitivity-enhancing detection method of a colloidal gold immunochromatography is characterized by comprising the following steps:
obtaining a sample to be detected, and dripping a first preset volume of the sample to be detected into a sample adding hole of the colloidal gold immunochromatography kit for reaction for a first preset time;
dripping a sensitization reagent with a second preset volume into the sample adding hole to react for a second preset time;
and repeating the steps for multiple times until the reading change of the colloidal gold immunochromatographic kit is within a preset range, thereby completing the detection of the sample to be detected.
2. The method of claim 1, further comprising, before the dropping the first predetermined volume of the test sample into the loading well of the gold immunochromatographic kit:
and carrying out conventional pretreatment on the detection sample.
3. The method of claim 1, wherein the sensitizing agent comprises a first sensitizing agent, a second sensitizing agent, and a third sensitizing agent mixed in a predetermined volume ratio; wherein,
the first sensitization reagent is a chloroauric acid solution with the weight percentage concentration of 2% -5%, the second sensitization reagent is a reduction solution, and the third sensitization reagent is a comparison solution.
4. The method according to claim 3, wherein the first sensitizing agent is a chloroauric acid solution having a concentration of 3% by weight.
5. The method according to claim 3, characterized in that the second sensitizing agent is a hydroxylamine hydrochloride solution having a molar concentration of 2% to 5% or an ascorbic acid solution having a weight percentage concentration of 0.3%.
6. The method according to claim 3, characterised in that the third sensitising agent is an aqueous solution of trisodium citrate with a concentration of 1% by weight.
7. Use of the method of any one of claims 1 to 6 for the detection of biological macromolecules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910386444.6A CN110275011A (en) | 2019-05-09 | 2019-05-09 | The sensitization detection method of colloidal gold immunity chromatography and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910386444.6A CN110275011A (en) | 2019-05-09 | 2019-05-09 | The sensitization detection method of colloidal gold immunity chromatography and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110275011A true CN110275011A (en) | 2019-09-24 |
Family
ID=67959036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910386444.6A Pending CN110275011A (en) | 2019-05-09 | 2019-05-09 | The sensitization detection method of colloidal gold immunity chromatography and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110275011A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101470114A (en) * | 2008-05-14 | 2009-07-01 | 中国检验检疫科学研究院 | Sensitization detection method of colloidal gold immunity chromatography and use thereof |
| CN101470116A (en) * | 2008-06-12 | 2009-07-01 | 中国检验检疫科学研究院 | Colloidal gold immunity percolation sensitization method for detecting avian influenza virus and its reagent kit |
| CN102778559A (en) * | 2012-08-14 | 2012-11-14 | 广州万孚生物技术股份有限公司 | Colloidal gold immunochromatographic detection kit and preparation method thereof |
| WO2014082439A1 (en) * | 2012-11-30 | 2014-06-05 | 中国农业科学院油料作物研究所 | Method for improving detection sensitivity of colloidal gold immunochromatographic test strip |
-
2019
- 2019-05-09 CN CN201910386444.6A patent/CN110275011A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101470114A (en) * | 2008-05-14 | 2009-07-01 | 中国检验检疫科学研究院 | Sensitization detection method of colloidal gold immunity chromatography and use thereof |
| CN101470116A (en) * | 2008-06-12 | 2009-07-01 | 中国检验检疫科学研究院 | Colloidal gold immunity percolation sensitization method for detecting avian influenza virus and its reagent kit |
| CN102778559A (en) * | 2012-08-14 | 2012-11-14 | 广州万孚生物技术股份有限公司 | Colloidal gold immunochromatographic detection kit and preparation method thereof |
| WO2014082439A1 (en) * | 2012-11-30 | 2014-06-05 | 中国农业科学院油料作物研究所 | Method for improving detection sensitivity of colloidal gold immunochromatographic test strip |
Non-Patent Citations (1)
| Title |
|---|
| 王景云: "提高免疫层析试纸条灵敏度方法的研究", 《中国优秀博硕士学位论文全文数据库(硕士)——工程科技I辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106855572A (en) | A kind of gastrin-releasing peptide precursor chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN107044977A (en) | A kind of tyrosine phosphatase antibody chemical luminescence immunity detection reagent and preparation method thereof | |
| CN114216897A (en) | sST2 chemiluminescence detection kit and detection method thereof | |
| CN111289758B (en) | Kit for quantitative detection of H-FABP and method for quantitative detection of H-FABP | |
| Gholve et al. | Comparison of serum thyroglobulin levels in differentiated thyroid cancer patients using in-house developed radioimmunoassay and immunoradiometric procedures | |
| CN111239404B (en) | Detection kit capable of simultaneously detecting retinol binding protein in urine sample and serum sample | |
| CN104459138A (en) | Detection kit for insulin-like growth factor binding protein-1 | |
| Premjeet et al. | Enzyme-Linked Immuno-Sorbent Assay (ELISA), basics and it’s application: A comprehensive review | |
| CN107966563A (en) | A kind of antimyeloperoxidase antibody IgG chemiluminescence immunoassay kits and preparation method thereof | |
| CN106198959A (en) | Serum hyaluronic acid chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN110275011A (en) | The sensitization detection method of colloidal gold immunity chromatography and application | |
| CN106124776A (en) | CA724 chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN111007248A (en) | Chemiluminescence immunoassay kit for detecting rubella virus IgM antibody | |
| CN106248943A (en) | Antinuclear antibody chemiluminescence immune detection reagent kit and preparation method thereof | |
| Luppa et al. | Pre-evaluation and system optimization of the Elecsys® thyroid electrochemiluminescence immunoassays | |
| CN106405098A (en) | An anti-beta2 glycoprotein I antibody chemiluminescence immunodetection kit and a preparing method thereof | |
| CN110618280A (en) | Thyrotropin determination kit and preparation method thereof | |
| CN113113079B (en) | Method for identifying hook effect in quantitative immunochromatography test | |
| WO2022065398A1 (en) | Ferritin measuring reagent | |
| CN106645759A (en) | Aldosterone chemiluminescent immunodetection kit and preparation method thereof | |
| CN115078705A (en) | Alzheimer disease biomarker chemiluminescence assay kit | |
| CN111537751A (en) | Myocardial troponin I, creatine kinase isoenzyme and myoglobin joint detection kit and detection method | |
| Haver et al. | Semiquantitation of cannabinoid immunoassays? A reexamination of the EMIT 20-ng/mL assay | |
| CN117871864A (en) | ELISA method-based method for detecting anti-PEG anti-drug antibody | |
| JP2010117244A (en) | Method for measuring hemoglobin and kit for measurement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |