CN109748921B - N-tert-butyloxycarbonyl protected heterocyclic compound, preparation method thereof and method for preparing C-nucleoside analogue by using N-tert-butyloxycarbonyl protected heterocyclic compound - Google Patents
N-tert-butyloxycarbonyl protected heterocyclic compound, preparation method thereof and method for preparing C-nucleoside analogue by using N-tert-butyloxycarbonyl protected heterocyclic compound Download PDFInfo
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- CN109748921B CN109748921B CN201711071763.5A CN201711071763A CN109748921B CN 109748921 B CN109748921 B CN 109748921B CN 201711071763 A CN201711071763 A CN 201711071763A CN 109748921 B CN109748921 B CN 109748921B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 30
- 229940127073 nucleoside analogue Drugs 0.000 title claims abstract description 27
- 150000002391 heterocyclic compounds Chemical class 0.000 title claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 101
- 239000000460 chlorine Substances 0.000 claims abstract description 90
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 49
- 239000002777 nucleoside Substances 0.000 claims abstract description 31
- 229940125898 compound 5 Drugs 0.000 claims abstract description 16
- 229910052736 halogen Inorganic materials 0.000 claims abstract description 12
- 150000002367 halogens Chemical class 0.000 claims abstract description 12
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 10
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims abstract description 9
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 claims abstract description 9
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910052794 bromium Inorganic materials 0.000 claims abstract description 9
- 125000004093 cyano group Chemical group *C#N 0.000 claims abstract description 9
- 239000011630 iodine Substances 0.000 claims abstract description 9
- 229910052740 iodine Inorganic materials 0.000 claims abstract description 9
- 125000003342 alkenyl group Chemical group 0.000 claims abstract description 8
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 7
- 238000006243 chemical reaction Methods 0.000 claims description 189
- 150000001875 compounds Chemical class 0.000 claims description 117
- 239000003153 chemical reaction reagent Substances 0.000 claims description 59
- MZRVEZGGRBJDDB-UHFFFAOYSA-N N-Butyllithium Chemical compound [Li]CCCC MZRVEZGGRBJDDB-UHFFFAOYSA-N 0.000 claims description 58
- -1 lactone compound Chemical class 0.000 claims description 55
- ZCSHNCUQKCANBX-UHFFFAOYSA-N lithium diisopropylamide Chemical compound [Li+].CC(C)[N-]C(C)C ZCSHNCUQKCANBX-UHFFFAOYSA-N 0.000 claims description 53
- 229910052751 metal Inorganic materials 0.000 claims description 47
- 239000002184 metal Substances 0.000 claims description 47
- FTVLMFQEYACZNP-UHFFFAOYSA-N trimethylsilyl trifluoromethanesulfonate Chemical compound C[Si](C)(C)OS(=O)(=O)C(F)(F)F FTVLMFQEYACZNP-UHFFFAOYSA-N 0.000 claims description 29
- 238000007259 addition reaction Methods 0.000 claims description 26
- 229940125782 compound 2 Drugs 0.000 claims description 20
- KZMGYPLQYOPHEL-UHFFFAOYSA-N Boron trifluoride etherate Chemical compound FB(F)F.CCOCC KZMGYPLQYOPHEL-UHFFFAOYSA-N 0.000 claims description 18
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 18
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 17
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims description 16
- 239000003638 chemical reducing agent Substances 0.000 claims description 16
- 150000002596 lactones Chemical class 0.000 claims description 16
- AQRLNPVMDITEJU-UHFFFAOYSA-N triethylsilane Chemical compound CC[SiH](CC)CC AQRLNPVMDITEJU-UHFFFAOYSA-N 0.000 claims description 16
- 239000011968 lewis acid catalyst Substances 0.000 claims description 12
- 239000002841 Lewis acid Substances 0.000 claims description 10
- 150000007517 lewis acids Chemical class 0.000 claims description 10
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 9
- 230000002829 reductive effect Effects 0.000 claims description 9
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 claims description 7
- 229940125904 compound 1 Drugs 0.000 claims description 5
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims description 4
- 229920002554 vinyl polymer Polymers 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 2
- 229930182470 glycoside Natural products 0.000 claims 1
- 150000002338 glycosides Chemical class 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 30
- 239000002994 raw material Substances 0.000 abstract description 14
- 238000003786 synthesis reaction Methods 0.000 abstract description 11
- 125000000304 alkynyl group Chemical group 0.000 abstract description 7
- 238000000746 purification Methods 0.000 abstract description 7
- 230000002194 synthesizing effect Effects 0.000 abstract description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 353
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 250
- 239000000243 solution Substances 0.000 description 226
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 176
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 141
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 136
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 119
- 238000003756 stirring Methods 0.000 description 117
- 239000012044 organic layer Substances 0.000 description 98
- 235000019439 ethyl acetate Nutrition 0.000 description 97
- 238000001816 cooling Methods 0.000 description 94
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 88
- 238000004809 thin layer chromatography Methods 0.000 description 82
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 76
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical class [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 74
- 238000004896 high resolution mass spectrometry Methods 0.000 description 74
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 66
- 229920006395 saturated elastomer Polymers 0.000 description 62
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical class O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 59
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 53
- 239000000741 silica gel Substances 0.000 description 53
- 229910002027 silica gel Inorganic materials 0.000 description 53
- 239000000047 product Substances 0.000 description 51
- 238000010791 quenching Methods 0.000 description 46
- 239000012043 crude product Substances 0.000 description 45
- 239000010410 layer Substances 0.000 description 44
- 239000003208 petroleum Substances 0.000 description 44
- 238000001514 detection method Methods 0.000 description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 40
- DLEDOFVPSDKWEF-UHFFFAOYSA-N lithium butane Chemical compound [Li+].CCC[CH2-] DLEDOFVPSDKWEF-UHFFFAOYSA-N 0.000 description 38
- 239000002904 solvent Substances 0.000 description 35
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 33
- 238000005160 1H NMR spectroscopy Methods 0.000 description 30
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 30
- 239000007787 solid Substances 0.000 description 30
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 27
- 238000000605 extraction Methods 0.000 description 27
- 238000001035 drying Methods 0.000 description 26
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 25
- 238000005406 washing Methods 0.000 description 23
- 150000002170 ethers Chemical class 0.000 description 22
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 230000000171 quenching effect Effects 0.000 description 21
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 20
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 19
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical group CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 18
- 239000000203 mixture Substances 0.000 description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 16
- 235000017557 sodium bicarbonate Nutrition 0.000 description 16
- 239000007864 aqueous solution Substances 0.000 description 15
- 229910000104 sodium hydride Inorganic materials 0.000 description 15
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 14
- 238000004128 high performance liquid chromatography Methods 0.000 description 14
- XHFLOLLMZOTPSM-UHFFFAOYSA-M sodium;hydrogen carbonate;hydrate Chemical class [OH-].[Na+].OC(O)=O XHFLOLLMZOTPSM-UHFFFAOYSA-M 0.000 description 14
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 12
- IUYHWZFSGMZEOG-UHFFFAOYSA-M magnesium;propane;chloride Chemical compound [Mg+2].[Cl-].C[CH-]C IUYHWZFSGMZEOG-UHFFFAOYSA-M 0.000 description 12
- 239000012265 solid product Substances 0.000 description 12
- JWUJQDFVADABEY-UHFFFAOYSA-N 2-methyltetrahydrofuran Chemical compound CC1CCCO1 JWUJQDFVADABEY-UHFFFAOYSA-N 0.000 description 11
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 description 11
- 238000010438 heat treatment Methods 0.000 description 11
- 239000012046 mixed solvent Substances 0.000 description 11
- 230000035484 reaction time Effects 0.000 description 11
- 230000015572 biosynthetic process Effects 0.000 description 9
- PSHKMPUSSFXUIA-UHFFFAOYSA-N n,n-dimethylpyridin-2-amine Chemical compound CN(C)C1=CC=CC=N1 PSHKMPUSSFXUIA-UHFFFAOYSA-N 0.000 description 9
- 239000007818 Grignard reagent Substances 0.000 description 8
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Inorganic materials [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 8
- DBTNVRCCIDISMV-UHFFFAOYSA-L lithium;magnesium;propane;dichloride Chemical compound [Li+].[Mg+2].[Cl-].[Cl-].C[CH-]C DBTNVRCCIDISMV-UHFFFAOYSA-L 0.000 description 8
- 239000002773 nucleotide Substances 0.000 description 8
- 239000012074 organic phase Substances 0.000 description 8
- RUHJZSZTSCSTCC-UHFFFAOYSA-N 2-(bromomethyl)naphthalene Chemical group C1=CC=CC2=CC(CBr)=CC=C21 RUHJZSZTSCSTCC-UHFFFAOYSA-N 0.000 description 7
- 125000002524 organometallic group Chemical group 0.000 description 7
- 150000003839 salts Chemical class 0.000 description 7
- 235000002639 sodium chloride Nutrition 0.000 description 7
- JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 6
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 6
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 6
- 239000002585 base Substances 0.000 description 6
- 229940126214 compound 3 Drugs 0.000 description 6
- 239000005457 ice water Substances 0.000 description 6
- DYHSDKLCOJIUFX-UHFFFAOYSA-N tert-butoxycarbonyl anhydride Chemical group CC(C)(C)OC(=O)OC(=O)OC(C)(C)C DYHSDKLCOJIUFX-UHFFFAOYSA-N 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 5
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical group [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 5
- 239000012267 brine Substances 0.000 description 5
- 239000012312 sodium hydride Chemical group 0.000 description 5
- ZSGJKBKUYXDVIE-UHFFFAOYSA-N CC(C)C[Mg] Chemical compound CC(C)C[Mg] ZSGJKBKUYXDVIE-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
- 150000001335 aliphatic alkanes Chemical class 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- CCZVEWRRAVASGL-UHFFFAOYSA-N lithium;2-methanidylpropane Chemical compound [Li+].CC(C)[CH2-] CCZVEWRRAVASGL-UHFFFAOYSA-N 0.000 description 4
- UBJFKNSINUCEAL-UHFFFAOYSA-N lithium;2-methylpropane Chemical compound [Li+].C[C-](C)C UBJFKNSINUCEAL-UHFFFAOYSA-N 0.000 description 4
- IWCVDCOJSPWGRW-UHFFFAOYSA-M magnesium;benzene;chloride Chemical compound [Mg+2].[Cl-].C1=CC=[C-]C=C1 IWCVDCOJSPWGRW-UHFFFAOYSA-M 0.000 description 4
- NXPHGHWWQRMDIA-UHFFFAOYSA-M magnesium;carbanide;bromide Chemical compound [CH3-].[Mg+2].[Br-] NXPHGHWWQRMDIA-UHFFFAOYSA-M 0.000 description 4
- DVSDBMFJEQPWNO-UHFFFAOYSA-N methyllithium Chemical compound C[Li] DVSDBMFJEQPWNO-UHFFFAOYSA-N 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- NHKJPPKXDNZFBJ-UHFFFAOYSA-N phenyllithium Chemical compound [Li]C1=CC=CC=C1 NHKJPPKXDNZFBJ-UHFFFAOYSA-N 0.000 description 4
- 229920001843 polymethylhydrosiloxane Polymers 0.000 description 4
- 125000001981 tert-butyldimethylsilyl group Chemical group [H]C([H])([H])[Si]([H])(C([H])([H])[H])[*]C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 4
- 125000000037 tert-butyldiphenylsilyl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1[Si]([H])([*]C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 4
- 125000000025 triisopropylsilyl group Chemical group C(C)(C)[Si](C(C)C)(C(C)C)* 0.000 description 4
- MOHYOXXOKFQHDC-UHFFFAOYSA-N 1-(chloromethyl)-4-methoxybenzene Chemical compound COC1=CC=C(CCl)C=C1 MOHYOXXOKFQHDC-UHFFFAOYSA-N 0.000 description 3
- NYHBQMYGNKIUIF-BDXYJKHTSA-N 2-amino-9-[(2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one Chemical compound NC1=NC(O)=C2N=CN([C@H]3O[C@H](CO)[C@@H](O)[C@H]3O)C2=N1 NYHBQMYGNKIUIF-BDXYJKHTSA-N 0.000 description 3
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 3
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 3
- 238000005481 NMR spectroscopy Methods 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 230000000840 anti-viral effect Effects 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- YTJXGDYAEOTOCG-UHFFFAOYSA-N lithium;di(propan-2-yl)azanide;oxolane Chemical compound [Li+].C1CCOC1.CC(C)[N-]C(C)C YTJXGDYAEOTOCG-UHFFFAOYSA-N 0.000 description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 3
- 238000005580 one pot reaction Methods 0.000 description 3
- 235000020030 perry Nutrition 0.000 description 3
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 3
- 125000001424 substituent group Chemical group 0.000 description 3
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 2
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical compound ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 2
- BTNTUKGYZFJZLU-UHFFFAOYSA-L CC1(NC(CCC1)(C)C)C.[Cl-].[Cl-].[Li+].[Li+] Chemical compound CC1(NC(CCC1)(C)C)C.[Cl-].[Cl-].[Li+].[Li+] BTNTUKGYZFJZLU-UHFFFAOYSA-L 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 229940126656 GS-4224 Drugs 0.000 description 2
- 229940124683 HCV polymerase inhibitor Drugs 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical class [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- YTDDBKIYDJABKW-UHFFFAOYSA-L [Cl-].[Li+].[Cl-].CC1(NC(CCC1)(C)C)C.[Mg+2] Chemical compound [Cl-].[Li+].[Cl-].CC1(NC(CCC1)(C)C)C.[Mg+2] YTDDBKIYDJABKW-UHFFFAOYSA-L 0.000 description 2
- QQIRAVWVGBTHMJ-UHFFFAOYSA-N [dimethyl-(trimethylsilylamino)silyl]methane;lithium Chemical compound [Li].C[Si](C)(C)N[Si](C)(C)C QQIRAVWVGBTHMJ-UHFFFAOYSA-N 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- IJOOHPMOJXWVHK-UHFFFAOYSA-N chlorotrimethylsilane Chemical compound C[Si](C)(C)Cl IJOOHPMOJXWVHK-UHFFFAOYSA-N 0.000 description 2
- DOBRDRYODQBAMW-UHFFFAOYSA-N copper(i) cyanide Chemical compound [Cu+].N#[C-] DOBRDRYODQBAMW-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010537 deprotonation reaction Methods 0.000 description 2
- QMMFVYPAHWMCMS-UHFFFAOYSA-N dimethylsulfide Substances CSC QMMFVYPAHWMCMS-UHFFFAOYSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- SRCZQMGIVIYBBJ-UHFFFAOYSA-N ethoxyethane;ethyl acetate Chemical compound CCOCC.CCOC(C)=O SRCZQMGIVIYBBJ-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- CCERQOYLJJULMD-UHFFFAOYSA-M magnesium;carbanide;chloride Chemical compound [CH3-].[Mg+2].[Cl-] CCERQOYLJJULMD-UHFFFAOYSA-M 0.000 description 2
- YAMQOOCGNXAQGW-UHFFFAOYSA-M magnesium;methylbenzene;bromide Chemical compound [Mg+2].[Br-].CC1=CC=CC=[C-]1 YAMQOOCGNXAQGW-UHFFFAOYSA-M 0.000 description 2
- LQVSLLSEXLZRRH-UHFFFAOYSA-M magnesium;methylbenzene;chloride Chemical compound [Mg+2].[Cl-].CC1=CC=CC=[C-]1 LQVSLLSEXLZRRH-UHFFFAOYSA-M 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 150000003833 nucleoside derivatives Chemical class 0.000 description 2
- ANRQGKOBLBYXFM-UHFFFAOYSA-M phenylmagnesium bromide Chemical compound Br[Mg]C1=CC=CC=C1 ANRQGKOBLBYXFM-UHFFFAOYSA-M 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- NTTOTNSKUYCDAV-UHFFFAOYSA-N potassium hydride Chemical compound [KH] NTTOTNSKUYCDAV-UHFFFAOYSA-N 0.000 description 2
- 229910000105 potassium hydride Inorganic materials 0.000 description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 229910000144 sodium(I) superoxide Inorganic materials 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- UJJDEOLXODWCGK-UHFFFAOYSA-N tert-butyl carbonochloridate Chemical compound CC(C)(C)OC(Cl)=O UJJDEOLXODWCGK-UHFFFAOYSA-N 0.000 description 2
- ITMCEJHCFYSIIV-UHFFFAOYSA-M triflate Chemical compound [O-]S(=O)(=O)C(F)(F)F ITMCEJHCFYSIIV-UHFFFAOYSA-M 0.000 description 2
- 238000000825 ultraviolet detection Methods 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 208000016057 CHAND syndrome Diseases 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002160 Celluloid Polymers 0.000 description 1
- 201000011001 Ebola Hemorrhagic Fever Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001125671 Eretmochelys imbricata Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 102000011787 Histone Methyltransferases Human genes 0.000 description 1
- 108010036115 Histone Methyltransferases Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 102000016397 Methyltransferase Human genes 0.000 description 1
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000404277 Sergentomyia babu Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- YAAQYJCOIFNMKX-RSTNYOGXSA-N [(2r,3r,4r,5r)-5-(4-aminopyrrolo[2,1-f][1,2,4]triazin-7-yl)-5-cyano-4-hydroxy-4-methyl-2-[[[[(2s)-1-oxo-1-propan-2-yloxypropan-2-yl]amino]-phenoxyphosphoryl]oxymethyl]oxolan-3-yl] 2-methylpropanoate Chemical compound O([P@@](=O)(OC[C@@H]1[C@H]([C@@](C)(O)[C@](C#N)(C=2N3N=CN=C(N)C3=CC=2)O1)OC(=O)C(C)C)N[C@@H](C)C(=O)OC(C)C)C1=CC=CC=C1 YAAQYJCOIFNMKX-RSTNYOGXSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 229940121357 antivirals Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- HSDAJNMJOMSNEV-UHFFFAOYSA-N benzyl chloroformate Chemical compound ClC(=O)OCC1=CC=CC=C1 HSDAJNMJOMSNEV-UHFFFAOYSA-N 0.000 description 1
- FHRRJZZGSJXPRQ-UHFFFAOYSA-N benzyl phenylmethoxycarbonyl carbonate Chemical compound C=1C=CC=CC=1COC(=O)OC(=O)OCC1=CC=CC=C1 FHRRJZZGSJXPRQ-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
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- 238000012925 biological evaluation Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 241000902900 cellular organisms Species 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- WHHGLZMJPXIBIX-UHFFFAOYSA-N decabromodiphenyl ether Chemical compound BrC1=C(Br)C(Br)=C(Br)C(Br)=C1OC1=C(Br)C(Br)=C(Br)C(Br)=C1Br WHHGLZMJPXIBIX-UHFFFAOYSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 1
- 125000000555 isopropenyl group Chemical group [H]\C([H])=C(\*)C([H])([H])[H] 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 1
- 229910052808 lithium carbonate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 125000004370 n-butenyl group Chemical group [H]\C([H])=C(/[H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 235000020004 porter Nutrition 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 229940002612 prodrug Drugs 0.000 description 1
- 239000000651 prodrug Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- RWWYLEGWBNMMLJ-MEUHYHILSA-N remdesivir Drugs C([C@@H]1[C@H]([C@@H](O)[C@@](C#N)(O1)C=1N2N=CN=C(N)C2=CC=1)O)OP(=O)(N[C@@H](C)C(=O)OCC(CC)CC)OC1=CC=CC=C1 RWWYLEGWBNMMLJ-MEUHYHILSA-N 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- WRIKHQLVHPKCJU-UHFFFAOYSA-N sodium bis(trimethylsilyl)amide Chemical compound C[Si](C)(C)N([Na])[Si](C)(C)C WRIKHQLVHPKCJU-UHFFFAOYSA-N 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000000707 stereoselective effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 125000000026 trimethylsilyl group Chemical group [H]C([H])([H])[Si]([*])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
Landscapes
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the field of chemical synthesis, and particularly discloses a N-tert-butyloxycarbonyl protected heterocyclic compound shown in a general formula 5, a preparation method thereof and a method for preparing a C-nucleoside analogue by using the N-tert-butyloxycarbonyl protected heterocyclic compound. In the compound 5, Hal is selected from chlorine, bromine and iodine; z is selected from CR0Or nitrogen, R0Is H, halogen, cyano, C1‑C6Straight or branched alkyl, C2‑C6Straight or branched alkenyl or C2‑C6Straight or branched alkynyl. In the prior literature, the synthesized C-nucleosides are mainly beta-C-nucleoside products, but beta: alpha has unsatisfactory stereoselectivity, and has the problems of separation and purification of products and consumption of expensive synthetic raw materials. The method provided by the invention starts from the compound 5 which is easy to prepare and low in cost, and can be used for controllably synthesizing the alpha-C-nucleoside product 8 or the beta-C-nucleoside product 3, and has the advantages of good stereoselectivity, high yield and good repeatability.
Description
Technical Field
The invention belongs to the field of chemical synthesis, and particularly relates to N-tert-butyloxycarbonyl (Boc) protected heterocyclic compounds (compounds shown in a general formula 5), a preparation method thereof and a method for preparing C-nucleoside analogues by using the same.
Background
C-nucleosides have gained extensive research interest due to their superior druggability, and the C-nucleoside structures shown in the following documents all show superior antiviral or antitumor pharmacological activity. There are many reports on methods for the synthesis of C-nucleoside analogues, but the synthesis of some specific C-nucleosides, as shown in the following documents, is still difficult.
Gilead company reported in 2012 that bases 1-1a and 1-2a can be added and reduced with ribolactone 2-1a after being temporarily protected by silicon to obtain C-nucleoside analogs 3a and 3b [ 1) Cho, A.; zhang, l.; xu, j.; babusis, d.; butler, t.; lee, r.; saunders, o.l.; wang, t.; parrish, J.; perry, j.; feng, j.y.; ray, a.s.; kim, c.u., Synthesis and catalysis of 2' -C-Me branched C-nucleotides as HCV polymerase inhibitors bioorganic & Medicinal Chemistry Letters 2012,22(12), 4127-4132.2) Cho, a; zhang, l.; xu, j.; lee, r.; butler, t.; metobo, s.; aktoutianakis, v.; lew, w.; ye, h.; clarke, m.; doerfler, e.; byun, d.; wang, t.; babusis, d.; carey, a.c.; german, p.; sauer, d.; zhong, w.; rossi, s.; finaux, m.; McHutchison, j.g.; perry, j.; feng, j.; ray, a, s.; kim, C.U., Discovery of the First C-nucleotide HCV Polymerase Inhibitor (GS-6620) with a purified antibiotic Response in HCV Infected Patients. journal of Medicinal Chemistry 2014,57(5), 1812. 1825.
The Gilead company in 2017 reported an improvement of this method: starting from bases 1-1b, the C-nucleoside analog 4a [ Siegel, d.; hui, h.c.; doerfler, e.; clarke, m.o.; chun, k.; zhang, l.; neville, s.; carra, e.; lew, w.; ross, b.; wang, q.; wolfe, l.; jordan, r.; soloveva, v.; knox, j.; perry, j.; perron, m.; stray, k.m.; barauskas, o.; feng, j.y.; xu, y.; lee, g.; rheingold, a, l.; ray, a.s.; banister, r.; strickley, r.; swaminathan, s.; lee, w.a.; bavari, s.; cihler, t.; lo, m.k.; warren, t.k.; mackman, R.L., Discovery and Synthesis of phosphate Prodrug of a Pyrrolo [2,1-f ] [ triazin-4-amino ] Adenine C-nucleotide (GS-5734) for the Treatment of Ebola and ignition viruses journal of medical Chemistry 2017,60(5),1648-1661 ].
More complicated synthetic methods for the C-nucleoside analogs 3-1a and 3-1b were reported in 2014 by the company Biota Scientific Management and Boehringer Ingelheim [ Draffan, a.g.; frey, b.; pool, b.; gannon, c.; tyndall, e.m.; lilly, m.; francom, p.; hufton, r.; halim, r.; jahangiri, s.; bond, s.; nguyen, v.t.t.; jeynes, t.p.; wirth, v.; luttick, a.; timanis, d.; thomas, j.d.; pryor, M.; porter, k.; morton, c.j.; lin, B.; duan, j.; kukolj, g.; simoneau, b.; McKercher, g.; langac é, l.; amad, m.a.; bethell, r.c.; tucker, S.P., Discovery and Synthesis of C-nucleotides as Potential New Anti-HCV Agents. ACS Medicinal Chemistry Letters 2014,5(6), 679-.
The above methods for synthesizing C-nucleoside analogs all have problems of long route, low yield, or poor repeatability, etc.
Meanwhile, according to literature reports, a variety of alpha-nucleosides have important biological activity or structural research values [ 1) Brown, k.l.; cheng, s.; zuo, x.; li, J.; chen, g.; valente, e.j.; zukkowski, j.d.; marques, H.M., Structural and Enzymic students of a New Analog of Coenzyme B12with an α -Adenosyl Upper Axial ligand, biochemistry 1998,37(27), 9704-; (2) gosselin, g.; bergogne, m.c.; imbach, J.L., Stereospermic synthesis and biological evaluation of 9-alpha-D-ribofuranosylguanine (alpha-guanosine). Nucleotides 1990,9(1), 81-7; (3) reitz, a.b.; rebarchak, m.c., Expeditious and stereoselective synthesis of α -guanosine, Nucleotides 1992,11(5), 1115-21; (4) secreist, j.a., III; parker, w.b.; allan, P.W.; bennett, l.l., jr.; roud, w.r.; truss, j.w.; fowler, a.t.; montgomery, j.a.; ealick, S.E.; wells, a.h.; gillespie, g.y.; gadi, v.k.; sorscher, E.J., Gene therapy of cancer: activation of nucleotide precursors with E.coli Gene protease Nucleotides 1999,18(4&5),745-757, the alpha-C-nucleoside analogs of which are of corresponding research interest. However, since the synthesis of alpha-C-nucleoside analogs is more difficult than the synthesis of C-nucleosides in general, studies on their application have been greatly limited.
Disclosure of Invention
The invention aims to provide N-tert-butyloxycarbonyl protected heterocyclic compounds which are intermediate compounds shown as a general formula 5 and are used for preparing C-nucleoside analogues.
Another object of the present invention is to provide a method for preparing the compound represented by the general formula 5.
Another object of the present invention is to provide the use of the compound represented by the general formula 5 for the preparation of C-nucleoside analogues.
In a first aspect of the present invention, there is provided a compound having the following formula 5:
wherein,
hal is selected from chlorine, bromine and iodine;
z is selected from CR0Or nitrogen, R0Is H, halogen, cyano, C1-C6Straight or branched alkyl, C2-C6Straight or branched alkenyl or C2-C6Straight or branched alkynyl.
Preferably, the compound represented by the formula 5 is a compound having one of the following formulae:
wherein,
r is selected from H, halogen, cyano, C1-C6Straight or branched alkyl, C2-C6Straight or branched alkenyl or C2-C6Straight or branched alkynyl; hal is as defined in formula 5.
In the second aspect of the present invention, there is also provided a process for producing a compound represented by the general formula 5, which comprises subjecting an amino group of the heterocyclic compound 1 to an amino group protection reaction to obtain a compound represented by the general formula 5,
wherein,
hal is selected from chlorine, bromine and iodine;
z is selected from CR0Or nitrogen, R0Is H, halogen, cyano, C1-C6Straight or branched alkyl, C2-C6Straight or branched alkenyl or C2-C6Straight or branched alkynyl.
In a third aspect of the present invention, there is also provided a process for preparing a C-nucleoside analogue from a compound represented by the general formula 5, which comprises the steps of:
(c) removing N from the compound shown in the general formula 56-proton, performing halogen-metal exchange, performing addition reaction with lactone compound 2 to obtain compound 7, and reducing compound 7 to obtain alpha-C-nucleoside analogue 8;
or,
(d) removal of Compound 5 from N6Proton, performing halogen-metal exchange, performing addition reaction with lactone compound 2-1 to obtain compound 9, reducing compound 9, and removing N6-tert-butoxycarbonyl protection to give beta-C-nucleoside analogue 3;
wherein,
hal is selected from chlorine, bromine and iodine;
z is selected from CR0Or nitrogen, R0Is H, halogen, cyano, C1-C6Straight or branched alkyl, C2-C6Straight or branched alkenyl or C2-C6Straight or branched chain alkynyl;
R1selected from H or methyl;
R2selected from H or Boc;
pg is selected from 2-naphthylmethyl, 1-naphthylmethyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, triisopropylsilyl, triethylsilyl, benzyl, p-methoxybenzyl, substituted by halogen or C1-C6The benzyl group substituted with a linear or branched alkyl group is preferably a 2-naphthylmethyl group, a p-methoxybenzyl group.
In a preferred embodiment, the compound of formula 5 is prepared by the following method:
(a) the amino group of the heterocyclic compound 1 is subjected to amino protection reaction to obtain the compound shown as the general formula 5
Wherein,
hal is selected from chlorine, bromine and iodine;
z is selected from CR0Or nitrogen, R0Is H, halogen, cyano or vinyl.
In a preferred embodiment, the lactone compound 2 or the lactone compound 2-1 is prepared by the following method:
(b) hydroxyl of the sugar compound 6 and PgBr or PgCl are subjected to hydroxyl protection reaction to obtain a lactone compound 2; or hydroxyl of the sugar compound 6-1 and PgBr or PgCl are subjected to hydroxyl protection reaction to obtain a lactone compound 2-1;
R1selected from H or methyl;
pg is selected from 2-naphthylmethyl, 1-naphthylmethyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, triisopropylsilyl, triethylsilyl, benzyl, p-methoxybenzyl, substituted by halogen or C1-C6The linear or branched alkyl-substituted benzyl group is preferably a benzyl group, a 2-naphthylmethyl group or a p-methoxybenzyl group.
In a preferred embodiment, the beta-C-nucleoside analog 3 or alpha-C-nucleoside analog 8 is prepared by the following method:
(a) carrying out amino protection reaction on the amino of the heterocyclic compound 1 to obtain a compound shown as a general formula 5;
(b) hydroxyl of the sugar compound 6 and PgBr or PgCl are subjected to hydroxyl protection reaction to obtain a lactone compound 2;
or hydroxyl of the sugar compound 6-1 and PgBr or PgCl are subjected to hydroxyl protection reaction to obtain a lactone compound 2-1;
(c) removing N from the compound shown in the general formula 56-proton, performing halogen-metal exchange, performing addition reaction with lactone compound 2 to obtain compound 7, and reducing compound 7 to obtain alpha-C-nucleoside analogue 8;
or,
(d) removing N from the general formula 56Protons, halogen-metal exchange, with lactone compounds 2-1Carrying out addition reaction to obtain a compound 9, reducing the compound 9, and removing N6-tert-butoxycarbonyl protection to give beta-C-nucleoside analogue 3;
wherein,
hal is selected from bromine and iodine;
z is selected from CR0Or nitrogen, R0Is H, cyano or vinyl;
R1selected from H or methyl;
R2selected from H or Boc;
pg is benzyl, 2-naphthylmethyl or p-methoxybenzyl.
In a preferred embodiment of the present invention,
the step (c) is one selected from the following steps:
(c-a) removing N from Compound 5-1a6-proton, performing halogen-metal exchange, and performing addition reaction with lactone compound 2 to obtain compound 7-1, and reducing compound 7-1 to obtain alpha-C-nucleoside analogue 8-1;
or,
(c-b) removing N from the compound 5-2a6-proton, performing halogen-metal exchange, performing addition reaction with lactone compound 2 to obtain compound 7-2, and reducing compound 7-2 to obtain alpha-C-nucleoside analogue 8-2;
wherein,
R1selected from H or methyl;
R2selected from H or tert-butyloxycarbonyl;
pg is 2-naphthylmethyl or benzyl.
In a preferred embodiment of the present invention,
step (d) is one selected from the following steps:
(d-a) removing N from Compound 5-1a6Proton, performing halogen-metal exchange, performing addition reaction with lactone compound 2-1 to obtain compound 9-3, reducing compound 9-3, and removing N6-tert-butoxycarbonyl protection to give beta-C-nucleoside analogue 3-3;
or,
(d-b) removing N from Compound 5-2a6Proton, performing halogen-metal exchange, performing addition reaction with lactone compound 2-1 to obtain compound 9-4, reducing compound 9-4, and removing N6-tert-butoxycarbonyl protection to give beta-C-nucleoside analogue 3-4;
wherein,
R1selected from H or methyl;
R2selected from H or tert-butyloxycarbonyl;
pg is p-methoxybenzyl or benzyl.
More preferably still, the first and second liquid crystal compositions are,
in the step (a), the first step of the method,
the amino protection reaction is carried out in two steps of a1 and a 2;
step a 1: the compound 1 is used as a raw material to react with an amino protection reagent in the presence of a solvent a1, an alkali a1 and a catalyst to obtain a product a 1.
The solvent a1 can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, tert-butyl methyl ether (TBME), isopropyl ether, dioxane, 2-methyltetrahydrofuran and Tetrahydrofuran (THF); the solvent a1 is preferably tetrahydrofuran and dichloromethane;
the amino protecting reagent is selected from di-tert-butyl dicarbonate (Boc)2O), tert-butyl chloroformate (Boc-Cl), the amino protecting reagent preferably being Boc2O；
The base a1 is selected from pyridine, triethylamine, diisopropylethylamine and dimethylaminopyridine; the base a1 is preferably triethylamine;
the catalyst is dimethylamino pyridine;
the reaction temperature in the step a1 is between-30 ℃ and +60 ℃, and the reaction time is between 0.5 and 24 hours;
step a 2: taking the product a1 prepared in the step a1 as a raw material, and reacting the raw material with a solvent a2 in the presence of a base a2 to obtain a compound 5;
the solvent a2 can be a mixed solvent of one or more of ethers, alcohols and water; the ethers are selected from one or more of ethyl ether, TBME, isopropyl ether, dioxane, 2-methyltetrahydrofuran and tetrahydrofuran; the alcohol is selected from one or more of methanol, ethanol and isopropanol; the solvent a2 is preferably water;
the alkali a2 is selected from sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine, diisopropylethylamine and calcium hydroxide;
the concentration of the alkali a2 and the solvent a2 after mixing is selected from 1M to 20M, preferably 2M to 4M.
The reaction temperature in the step a2 is between 0 ℃ and +100 ℃, and the reaction time is between 0.5 hours and 24 hours.
In the step (b), the step (c),
the hydroxyl protection reaction is to take a sugar compound 6 or a sugar compound 6-1 as a raw material to react with a hydroxyl protection reagent PgBr or PgCl in the presence of a solvent b and alkali b to obtain a lactone compound 2 or a lactone compound 2-1;
the solvent b can be a mixed solvent of one or more of ethers and amides; the ethers are selected from one or more of ethyl ether, TBME, isopropyl ether, 2-methyltetrahydrofuran and tetrahydrofuran; the amide is selected from N, N-Dimethylformamide (DMF), N, N-Dimethylacetamide (DMF), and formamide; the solvent b is preferably tetrahydrofuran and N, N-dimethylformamide;
the hydroxyl protecting reagent PgBr or PgCl is selected from a bromization reagent PgBr and a chlorination reagent PgCl; the hydroxyl protecting reagent is preferably 2-naphthylmethyl bromide and p-methoxybenzyl chloride;
the base b is selected from pyridine, triethylamine, diisopropylethylamine, dimethylaminopyridine, sodium hydride NaH and sodium hexamethyldisilazide, and is preferably NaH;
the temperature of the hydroxyl protection reaction is between-30 ℃ and +150 ℃, and the reaction time is between 0.5 and 12 hours.
In the step (c),
the removal of N6The protonic reaction is carried out by reacting compound 5 with a metal-organic reagent c1 in the presence of a solvent c 1;
the solvent c1 can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the solvent b1 is preferably tetrahydrofuran.
The organometallic reagent c1 is characterized by sufficient alkalinity to remove N6Protons, but not so much as to produce a halogen-metal exchange reaction. The metal organic reagent c1 is selected from one or more of isopropyl magnesium chloride, methyl magnesium bromide, methyl magnesium chloride, isopropyl magnesium chloride-LiCl mixture (Turbo-Grignard reagent), isobutyl magnesium chloride-LiCl mixture, phenyl magnesium chloride, phenyl magnesium bromide, o-tolyl magnesium chloride, o-tolyl magnesium bromide, magnesium dichloride (2,2,6, 6-tetramethylpiperidine) lithium salt, diisopropylaminolithium, hexamethyldisilazane lithium, n-butyllithium, isobutyl lithium, tert-butyllithium, methyllithium, phenyllithium, sodium hydride, potassium hydride; when Hal in compound 5 is Br, the organometallic reagent c1 is preferably isopropylmagnesium chloride, lithium diisopropylamide. When Hal in compound 5 is I, the metal-organic reagent c1 is preferably sodium hydride, phenylmagnesium chloride.
The temperature of the deprotonation reaction is between-78 ℃ and +30 ℃, and the reaction time is between 0.5 and 12 hours.
The halogen-metal exchange reaction is carried out in the presence of a solvent c2 and a metal organic reagent c 2;
the solvent can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyltetrahydrofuran and tetrahydrofuran; the solvent is preferably tetrahydrofuran.
The metal organic reagent c2 is selected from one or more of isopropyl magnesium chloride, methyl magnesium bromide, isopropyl magnesium chloride-LiCl mixture (Turbo-Grignard reagent), isobutyl magnesium chloride-LiCl mixture, lithium dichloride (2,2,6, 6-tetramethylpiperidine) salt, lithium diisopropylamide, hexamethyldisilazane lithium alkyl, n-butyl lithium, isobutyl lithium, tert-butyl lithium, methyl lithium and phenyl lithium; when Hal in compound 5 is Br, the organometallic reagent c2 is preferably an isopropylmagnesium chloride-LiCl mixture (Turbo-Grignard reagent), n-butyllithium. When Hal in compound 5 is I, the organometallic reagent c2 is preferably an isopropylmagnesium chloride-LiCl mixture (Turbo-Grignard reagent).
The temperature of the halogen-metal exchange reaction c2 is between-100 ℃ and-40 ℃, and the reaction time is between 0.5 and 12 hours.
The addition reaction is carried out in the presence of a solvent c3 and a lactone compound 2;
pg in the substrate 2 of the reaction is selected from 2-naphthylmethyl, 1-naphthylmethyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, triisopropylsilyl, triethylsilyl, benzyl, p-methoxybenzyl, substituted by halogen or C1-C6The benzyl group substituted with a linear or branched alkyl group is preferably a 2-naphthylmethyl group, a p-methoxybenzyl group.
The solvent c3 can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the solvent is preferably tetrahydrofuran.
Preferably, the addition reaction involving the metal organic reagent is carried out in the presence of a lewis acid catalyst selected from CuI, cuprous triflate (CuOTf), CuCN, LiCl, CuBr-dimethylsulfide complex, boron trifluoride etherate, TMSCl, or the like.
The temperature of the addition reaction c3 is between-100 ℃ and-10 ℃, and the reaction time is between 0.5 and 12 hours.
The above removal of N6The proton reaction, halogen-metal exchange reaction, addition reaction are preferably carried out in a "one-pot" manner to give compound 7.
The reduction reaction is carried out by reacting compound 7 raw material with reducing agent C4 and Lewis acid C4 in the presence of solvent C4 to obtain alpha-C-nucleoside analogue 8;
the reducing agent c4 is selected from silicon hydride derivatives, such as triethylsilane and polymethylhydrosiloxane, preferably triethylsilane and polymethylhydrosiloxane;
the reductive reaction is carried out in the presence of a Lewis acid catalyst c4, wherein the Lewis acid catalyst c4 is selected from boron trifluoride diethyl etherate or trimethylsilyl trifluoromethanesulfonate (TMSOTf) and the like; the lewis acid catalyst is preferably trimethylsilyl trifluoromethanesulfonate (TMSOTf).
The solvent c4 can be a mixed solvent of one or more of ethers, toluene, hexane and halogenated alkanes; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the halogenated alkane is one or more of dichloromethane, 1, 2-dichloroethane, 1, 1-dichloroethane and chloroform. The solvent is preferably dichloromethane.
The temperature of the reductive reaction is between-100 ℃ and-40 ℃, and the reaction time is between 0.5 and 12 hours;
the reagents, conditions and steps described in step (c) can be used in step (c) or (c-b);
in the step (d), the step (c),
the removal of N6The proton reaction is in the solvent d1, taking a compound 5 as a raw material to react with a metal organic reagent d1 in the presence of a catalyst;
the solvent d1 can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the solvent d1 is preferably tetrahydrofuran.
The metal-organic reagent d1 is characterized by sufficient alkalinity to remove N6Protons, but not so much as to produce a halogen-metal exchange reaction. The metal organic reagent c1 is selected from one or more of isopropyl magnesium chloride, methyl magnesium bromide, methyl magnesium chloride, isopropyl magnesium chloride-LiCl mixture (Turbo-Grignard reagent), isobutyl magnesium chloride-LiCl mixture, phenyl magnesium chloride, phenyl magnesium bromide, o-tolyl magnesium chloride, o-tolyl magnesium bromide, magnesium dichloride (2,2,6, 6-tetramethylpiperidine) lithium salt, diisopropylaminolithium, hexamethyldisilazane lithium, n-butyllithium, isobutyl lithium, tert-butyllithium, methyllithium, phenyllithium, sodium hydride, potassium hydride; when Hal in compound 5 is Br, the organometallic reagent c1 is preferably isopropylmagnesium chloride, lithium diisopropylamide. When Hal in compound 5 is I, the metal-organic reagent c1 is preferably sodium hydride, phenylmagnesium chloride.
The temperature of the deprotonation reaction is between-78 ℃ and +30 ℃, and the reaction time is between 0.5 and 12 hours.
The halogen-metal exchange reaction is carried out with a metal organic reagent d2 in the presence of a solvent d 2;
the solvent d2 can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the solvent is preferably tetrahydrofuran.
The metal organic reagent d2 is selected from one or more of isopropyl magnesium chloride, methyl magnesium bromide, isopropyl magnesium chloride-LiCl mixture (Turbo-Grignard reagent), isobutyl magnesium chloride-LiCl mixture, lithium dichloride (2,2,6, 6-tetramethylpiperidine) salt, lithium diisopropylamide, hexamethyldisilazane lithium alkyl, n-butyl lithium, isobutyl lithium, tert-butyl lithium, methyl lithium and phenyl lithium;
when Hal in compound 5 is Br, the organometallic reagent d2 is preferably an isopropylmagnesium chloride-LiCl mixture (Turbo-Grignard reagent), n-butyllithium. When Hal in compound 5 is I, the organometallic reagent d2 is preferably an isopropylmagnesium chloride-LiCl mixture (Turbo-Grignard reagent).
The temperature of the halogen-metal exchange reaction is between-100 ℃ and-40 ℃, and the reaction time is between 0.5 hour and 12 hours.
The addition reaction is carried out with a lactone compound 2 in the presence of a solvent d 3;
pg in the lactone compound 2 in the reaction is selected from tert-butyldimethylsilyl, tert-butyldiphenylsilyl, triethylsilyl, triisopropylsilyl, trimethylsilyl, benzyl, p-methoxybenzyl, halogen or C1-C6Linear or branched alkyl-substituted benzyl, 1-naphthylmethyl, 2-naphthylmethyl, preferably 2-naphthylmethyl and p-methoxybenzyl.
The solvent d3 can be a mixed solvent of one or more of ethers, toluene, hexane and dichloromethane; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the solvent is preferably tetrahydrofuran.
Preferably, the addition reaction is carried out in the presence of a lewis acid catalyst d3, the lewis acid catalyst d3 being selected from CuI, cuprous triflate (CuOTf), CuCN, LiCl, CuBr-dimethyl sulfide complex, boron trifluoride diethyl etherate or TMSCl, etc.
The temperature of the addition reaction is between-100 ℃ and-10 ℃, and the reaction time is between 0.5 and 12 hours.
The above removal of N6The proton reaction, halogen-metal exchange reaction, addition reaction are preferably carried out in a "one-pot" manner to give compound 9.
The reduction reaction is to react a compound 9 with a reducing agent d4 and Lewis acid d4 in the presence of a solvent d4 to obtain beta-C-nucleoside analogue 3;
the reducing agent d4 is selected from silicon hydride derivatives, such as triethylsilane and polymethylhydrosiloxane, preferably triethylsilane and polymethylhydrosiloxane;
the Lewis acid catalyst d4 is selected from boron trifluoride diethyl etherate or trimethylsilyl trifluoromethanesulfonate (TMSOTf) and the like; the Lewis acid catalyst d4 is preferably boron trifluoride diethyl etherate.
The solvent d4 can be a mixed solvent of one or more of ethers, toluene, hexane, acetonitrile and halogenated alkanes; the ethers are selected from one or more of diethyl ether, TBME, isopropyl ether, 2-methyl tetrahydrofuran and tetrahydrofuran; the halogenated alkane is one or more of dichloromethane, 1, 2-dichloroethane, 1, 1-dichloroethane and chloroform. The solvent is preferably acetonitrile.
The temperature of the reductive reaction is between-100 ℃ and-40 ℃, and the reaction time is between 0.5 and 12 hours;
the reagents, conditions and procedures described in step (d) may be employed in step (d-a) or (d-b).
Among the terms used in the present invention, C-nucleoside analogs can be classified into alpha-C-nucleoside analogs and beta-C-nucleoside analogs according to the relative positions of the substituents at the 1-and 5-positions of the ribose on both sides of the ribose, specifically, when the substituents at the 1-and 5-positions of the ribose are on opposite sides of the ribose, the configuration of the nucleoside is defined as alpha-form, such as alpha-C-nucleoside analog 8; when the substituents at the 1-and 5-positions of the ribose are on the same side of the ribose, the configuration of the nucleoside is defined as beta-type, e.g., beta-C-nucleoside analog 3;
in the terminology used in the present invention, "C1-C6Straight or branched alkyl "refers to straight or branched chain alkyl groups containing 1 to 6 carbon atoms, including but not limited to methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-pentyl, and the like.
“C2-C6Straight-chain or branched alkenyl "means straight-chain or branched alkenyl having 1 to 6 carbon atomsIncluding, but not limited to, ethenyl, n-propenyl, isopropenyl, n-butenyl, n-pentenyl, and the like.
“C2-C6Straight or branched alkynyl "means straight or branched alkynyl containing 1 to 6 carbon atoms and includes, but is not limited to, ethynyl, n-propynyl, isopropynyl, n-butynyl, n-pentynyl, and the like.
"halogen" means fluorine, chlorine, bromine or iodine.
The term "one-pot" as used in the present invention means that the reaction product of the respective stages of the reaction is carried out in one step without isolation and purification.
Advantageous effects
Starting from a N-tert-butyloxycarbonyl protected heterocycle 5, the C-nucleoside analogue can be simply synthesized.
The inventors have found that when the method of the document [ Journal of Medicinal Chemistry 2014,57(5), 1812-; recent documents [ Journal of Medicinal Chemistry 2017,60(5),1648-1661 ] provide the same information as the applicants' findings, and synthesize the C-nucleoside 4a using 1-1b as a starting material; however, 1-1b itself is more difficult to synthesize and is much more expensive than 1-1 a. Meanwhile, other documents [ ACS Medicinal Chemistry Letters 2014,5(6), 679-. Meanwhile, the C-nucleosides synthesized in the above documents all have a predominant beta-C-nucleoside product, but the documents show that the beta: alpha is not ideal in stereoselectivity, not only causing the problems of separation and purification of products, but also consuming expensive synthetic raw materials.
The method can controllably synthesize the alpha-C-nucleoside product 8 or the beta-C-nucleoside product 3 from the compound 5 which is easy to prepare and has lower cost, has good stereoselectivity, higher yield and good repeatability, and shows remarkable advantages compared with the literature method.
Detailed Description
The invention will now be further illustrated, but is not limited, by the following specific examples.
Sample data were determined by the following instrument: the melting point is measured by an SGW X-4B melting point apparatus, and the temperature is not corrected; nuclear magnetic resonance hydrogen spectrum (1H NMR) by means of a Bruker Avance III 400, 500 or 600 NMR spectrometer, carbon NMR spectrum: (13C NMR) was measured on a Bruker Avance III 600 or 500 nuclear magnetic resonance apparatus with TMS as internal standard and chemical shift in ppm; mass spectra were determined on a Finnigan LCQ/DECA and Micromass Ultra Q-TOF (ESI) mass spectrometer; the liquid phase model used for purity analysis is Agilent 1260 definition; the developing WFH-203B three-purpose ultraviolet analyzer is used in the department of science, and the wavelength is 254nm and 365 nm.
Column chromatography silica gel (100-; the TLC silica gel plate is an HSGF-254 thin-layer chromatography silica gel plate produced by a tobacco chemical plant, the thickness of the chromatography plate used for thin-layer chromatography is 0.2 +/-0.03 mm, and the thickness of the thin-layer chromatography prefabricated plate used for prefabricated preparation is 0.4-0.5 mm; petroleum ether (boiling range 60-90 deg.C), dichloromethane, ethyl acetate, and methanol are analytically pure and provided by Shanghai Tantake technology, Inc. or national drug group chemical reagent, Inc., and the reagents and solvents used are not specially treated except for special description.
Compounds 1-1a were obtained from Shanghai Bigdi pharmaceutical science and technology, Inc., 1-1c, 1-1d, 1-2a and 1-2b were obtained from Shanghai Farmer Biotechnology, Inc.,
1-1b was prepared according to the Journal of Medicinal Chemistry 2017,60(5), 1648-1661;
6a and 6-1c were prepared according to the method in Tetrahedron: Asymmetry 2007,18, 500-;
6b and 6-1d were prepared according to the method in org. Synth.2005,82, 75-79.
The optical rotation (+/-) was measured by an OR-2090 chiral detector (Hg-Xe lamp, 150W) manufactured by Japan chemical Co., Ltd. (JASCO).
High Performance Liquid Chromatography (HPLC) assay conditions: agilent 1260 analytical high performance liquid chromatography system (Agilent Inc.) and LC3000 preparative high performance liquid chromatography system (Beijing Innovation technology, Inc.).
Chiral OD or OJ columns were purchased from xylonite drug chiral technology (shanghai) ltd, column size 2cm Φ X25 cm.
Analytical high performance liquid chromatography conditions: c18 column (5 μm,4.6X 250mm), UV detection band 214 and 280nm, elution conditions 0-90% acetonitrile (containing 0.1% v/v TFA) gradient for 30 min. Preparing high performance liquid chromatography conditions: c18 column (5 μm,19X 250mm), UV detection band 214 and 280nm, elution conditions 0-90% acetonitrile (containing 0.1% v/v TFA) gradient 30 min.
In the above discussion and in the examples below, the following abbreviations have the following meanings. An abbreviation has a generally accepted meaning if it is not defined.
TLC is thin layer chromatography;
DMF is N, N-dimethylformamide;
EtOAc is ethyl acetate;
THF is tetrahydrofuran;
DMSO is dimethyl sulfoxide;
DCM is dichloromethane;
Et3n is triethylamine;
TBME is tert-butyl methyl ether;
Boc2o is di-tert-butyl dicarbonate;
Cbz2o is benzyloxy formic anhydride;
CbzCl is benzyl chloroformate;
PMB is p-methoxybenzyl;
TMSOTf is trimethylsilyl trifluoromethanesulfonate.
Example 1
Preparation of Compound 5-1 a:
compound 1-1a (5.0g,23.47mmol) was suspended in THF (100mL), triethylamine (16.3mL, 0.12mol) and dimethylaminopyridine (287mg,2.35mmol) were added, and Boc was added slowly in portions2O (15.4g,70.42mmol), stirred at room temperature overnight, after completion of the reaction, 3M aqueous sodium hydroxide (100mL) was added and the mixture was heated to room temperatureAfter stirring at 60 ℃ for 6 hours, the reaction mixture was allowed to return to room temperature, the organic layer was separated, the aqueous layer was extracted with ethyl acetate (20 mL. times.3), the organic phases were combined, the organic phase was washed with saturated brine (100mL), dried over anhydrous sodium sulfate, concentrated, and separated by silica gel column (10:1, V/V, petroleum ether: EtOAc) to give the product 5-1a (6.6g, 21.12mmol) as a white solid in yield: 90 percent.
M.p.180.0 ℃ (decomposition); rf0.21(10:1, V/V, petroleum ether: EtOAc); HPLC tR＝2.79min；1H NMR(400MHz,CDCl3)δ8.28(s,1H,ArH),8.24(s,1H,NH),7.39(d,J＝4.8Hz,1H,ArH), 6.90(d,J＝4.8Hz,1H,ArH),1.58(s,9H,C(CH3)3)；13C-NMR(126MHz,CDCl3)δ151.52 (C＝O),150.60,147.00,116.57,115.34,108.71,103.35,83.13(C(CH3)3),28.29(C(CH3)3) (ii) a High resolution mass spectrometry (ESI)+)：C11H13BrN4NaO2 +Theoretical value is 335.0114, found 335.0119.
Example 2
Preparation of Compound 5-1 b:
compound 1-1b (0.5g, 1.92mmol) was suspended in THF (10mL), triethylamine (1.3mL, 9.61mmol) and dimethylaminopyridine (23mg, 0.19mmol) were added, and Boc was added slowly in portions2O (1.3g, 5.77mmol), stirring at room temperature for reaction overnight, after the reaction is finished, adding 3M aqueous sodium hydroxide (10mL), heating to 60 ℃, stirring for reaction for 6 hours, returning to room temperature, separating an organic layer, extracting an aqueous layer by ethyl acetate (15mL x 3), combining the organic layers, washing the organic layer by saturated saline (30mL), drying by anhydrous sodium sulfate, concentrating, separating by silica gel column (10:1, V/V, petroleum ether: EtOAc), obtaining a product 5-1b (582mg, 1.62mmol), wherein the yield: 84 percent.
High resolution mass spectrometry (ESI)+)：C11H14IN4O2 +Theoretical value is 361.0156, found 361.0144.
Example 3
Preparation of Compounds 5-1 c:
compound 1-1c (0.5g, 2.10mmol) was suspended in THF (10mL), triethylamine (1.5mL, 10.50mmol) and dimethylaminopyridine (26mg, 0.21mmol) were added, and Boc was added slowly in portions2O (1.4g, 6.30 mmol), stirring at room temperature overnight, after the reaction is finished, adding 3M aqueous sodium hydroxide (10mL), heating to 60 ℃, stirring for reaction for 6 hours, returning to room temperature, separating an organic layer, extracting an aqueous layer with ethyl acetate (15mL x 3), combining the organic layers, washing the organic layer with saturated saline (30mL), drying with anhydrous sodium sulfate, concentrating, separating with silica gel column (10:1, V/V, petroleum ether: EtOAc), obtaining a product 5-1c (554mg, 1.64mmol), yield: 78 percent.
High resolution mass spectrometry (ESI)+)：C12H13BrN5O2 +Theoretical value is 338.0247, found 338.0234.
Example 4
Preparation of Compounds 5-1 d:
compound 1-1d (0.5g, 2.09mmol) was suspended in THF (10mL), triethylamine (1.5mL, 10.46mmol) and dimethylaminopyridine (26mg, 0.21mmol) were added, and Boc was added slowly in portions2O (1.4g, 6.27 mmol), stirring at room temperature for reaction overnight, after the reaction is finished, adding 3M aqueous sodium hydroxide solution (10mL), heating to 60 ℃, stirring for reaction for 6 hours, returning to room temperature, separating an organic layer, extracting an aqueous layer by ethyl acetate (15mL x 3), combining the organic layers, washing the organic layer by saturated saline (30mL), drying by anhydrous sodium sulfate, concentrating, separating by a silica gel column (10:1, V/V, petroleum ether: EtOAc), obtaining a product 5-1d (583mg, 1.71mmol), wherein the yield: 82 percent.
High resolutionMass spectrometry (ESI)+)：C13H16BrN4O2 +Theoretical value is 339.0451, found 339.0440.
Example 5
Preparation of Compound 5-2 a:
compound 1-2a (5.0g, 23.36mmol) was suspended in THF (100mL), triethylamine (16.3mL, 0.12mol) and dimethylaminopyridine (287mg,2.35mmol) were added, and Boc was added slowly in portions2O (15.4g,70.42mmol), stirring at room temperature for reaction overnight, after the reaction is finished, adding 3M aqueous sodium hydroxide solution (100mL), heating to 60 ℃, stirring for reaction for 6 hours, returning to room temperature, separating an organic layer, extracting an aqueous layer by ethyl acetate (20mL x 3), combining the organic layers, washing the organic layer by saturated saline (100mL), drying by anhydrous sodium sulfate, concentrating, separating by a silica gel column (10:1, V/V, petroleum ether: EtOAc), obtaining a white solid product 5-2a (6.3g, 20.09mmol), yield: 86 percent.
M.p. 160.5-163.8 deg.C (petroleum ether: EtOAc); rf0.29(5:1, V/V, petroleum ether: EtOAc); HPLC tR＝2.72min；1H-NMR(400MHz,CDCl3)δ8.62(s,1H,ArH),8.58(s,1H,NH),7.70(s,1H, ArH),1.57(s,9H,C(CH3)3)；13C-NMR(126MHz,CDCl3)δ149.51(C＝O),149.41,148.68, 133.48,129.16,103.44,83.87(C(CH3)3),28.14(C(CH3)3) (ii) a High resolution mass spectrometry (ESI)+)： C10H12BrN5NaO2 +Theoretical value is 336.0067, found 336.0058.
Example 6
Preparation of Compound 5-2 b:
compound 1-2b (0.2g, 0.77mmol) was suspendedTo THF (10mL) was added triethylamine (0.5mL, 3.83mmol) and dimethylaminopyridine (9mg, 0.08mmol) and the Boc was added slowly in portions2O (0.5g, 2.30mmol), stirring at room temperature for reaction overnight, after the reaction is finished, adding 3M aqueous sodium hydroxide solution (10mL), heating to 60 ℃, stirring for reaction for 6 hours, returning to room temperature, separating an organic layer, extracting an aqueous layer by ethyl acetate (15mL x 3), combining the organic layers, washing the organic layer by saturated saline (30mL), drying by anhydrous sodium sulfate, concentrating, separating by silica gel column (10:1, V/V, petroleum ether: EtOAc), obtaining a product 5-2b (194mg, 0.54mmol), wherein the yield: 70 percent.
High resolution mass spectrometry (ESI)+)：C10H13IN5O2 +Theoretical value is 362.0108, found 362.0103.
Example 7
Preparation of compound 2 a:
dissolving a raw material 6a (10.0g,49.46mmol) in anhydrous DMF (100mL), cooling to 0 ℃ in an ice bath, slowly adding 60% NaH (3.0g, 74.18mmol) in portions under the protection of nitrogen, stirring at 0 ℃ for reaction for 30 minutes, slowly adding 2-bromomethylnaphthalene (16.4g, 74.18mmol) in portions, removing the ice bath, heating to room temperature for stirring for reaction for 3 hours, after the reaction is completed, adding 200mL of ice water to quench the reaction, extracting with ethyl acetate (100mL x 4), combining organic phases, washing an organic layer by 100mL of saturated saline water, drying with anhydrous sodium sulfate, concentrating, separating by a silica gel column (4:1, V/V, petroleum ether: EtOAc) to obtain a white solid product 2a (15.1g, 44.10mmol), wherein the yield: 89 percent.
M.p. 68.8-70.2 deg.C (petroleum ether: EtOAc); rf0.27(5:1, V/V, petroleum ether: EtOAc); [ alpha ] to]D 20＝-15.46 (c＝0.705,CHCl3)；HPLC tR＝3.81min；1H-NMR(500MHz,CDCl3)δ7.87–7.80(m,3H, ArH),7.72(s,1H,ArH),7.53–7.45(m,2H,ArH),7.40(dd,J＝8.4,1.6Hz,1H,ArH),4.72 (d,J＝11.9Hz,1H,OCH2-),4.65(d,J＝11.9Hz,1H,OCH2-),4.57(t,J＝2.6Hz,1H,H-4), 4.51(s,1H,H-3),3.76(dd,J＝10.9,3.0Hz,1H,H-5),3.73(dd,J＝10.9,2.6Hz,1H,H-5’), 1.58(s,3H,2-CH3),1.43(s,3H,OC(CH3)2O),1.40(s,3H,OC(CH3)2O)；13C-NMR(126 MHz,CDCl3)δ176.43(C＝O),134.41(ArC),133.27(2x ArC),128.63(ArC),128.02(ArC), 127.86(ArC),127.14(ArC),126.47(ArC),126.35(ArC),125.80(ArC),112.90 (OC(CH3)2O),82.98(C-2),82.84(C-3),82.02(C-4),74.13(OCH2-),69.33(C-5),26.98 (OC(CH3)2O),26.88(OC(CH3)2O),19.95(2-CH3) (ii) a High resolution mass spectrometry (ESI)+)：C20H22NaO5 +Theoretical value is 365.1359, found 365.1366.
Example 8
Preparation of compound 2 b:
dissolving the raw material 6b (10.0g,53.14mmol) in anhydrous DMF (100mL), cooling to 0 ℃ in ice bath, slowly adding 60% NaH (3.2g, 79.71mmol) in portions under the protection of nitrogen, stirring at 0 ℃ for reaction for 30 minutes, slowly adding 2-bromomethylnaphthalene (17.6g, 79.71mmol) in portions, removing the ice bath, heating to room temperature, stirring for reaction for 3 hours, after the reaction is completed, adding 200mL of ice water to quench the reaction, extracting with ethyl acetate (100mL x 4), combining organic phases, washing an organic layer with 100mL of saturated saline water, drying with anhydrous sodium sulfate, concentrating, separating with a silica gel column (4:1, V/V, petroleum ether: to obtain a white solid product 2b (15.9g, 48.42mmol) with the yield: 91 percent.
M.p. 105.4-106.8 deg.C (petroleum ether: EtOAc); rf0.22(5:1, V/V, petroleum ether: EtOAc); [ alpha ] to]D 20＝-41.67(c＝0.240,CHCl3)；HPLC tR＝3.65min；1H NMR(500MHz,CDCl3)δ7.86– 7.81(m,3H,ArH),7.69(s,1H,ArH),7.51–7.47(m,2H,ArH),7.37(dd,J＝8.4,1.4Hz,1H, ArH),4.81(d,J＝5.5Hz,1H,H-2),4.73(d,J＝2.2Hz,1H,H-3),4.71(d,J＝4.0Hz,1H, OCH2-),4.65(t,J＝1.8Hz,1H,H-4),4.62(d,J＝11.9Hz,1H,OCH2-),3.75(dd,J＝10.7, 2.3Hz,1H,H-5),3.70(dd,J＝10.7,1.8Hz,1H,H-5’),1.47(s,3H,OC(CH3)2O),1.36(s,3H, OC(CH3)2O)；13C NMR(126MHz,CDCl3)δ174.47(1-C＝O),134.46(ArC),133.29(ArC), 133.28(ArC),128.72(ArC),128.04(ArC),127.87(ArC),126.93(ArC),126.50(ArC), 126.37(ArC),125.58(ArC),113.31((OC(CH3)2O),81.23(C-4),78.54(C-3),75.87(C-2), 74.19(OCH2-),69.17(C-5),26.95(OC(CH3)2O),25.76(OC(CH3)2O); high resolution mass spectrometry (ESI)+)： C19H20NaO5 +Theoretical value is 351.1203, found 351.1209.
Example 9
Preparation of compound 2 c:
dissolving a raw material 6a (1.0g,4.95mmol) in anhydrous DMF (20mL), cooling to 0 ℃ in an ice bath, slowly adding 60% NaH (0.3g, 7.42mmol) in portions under the protection of nitrogen, stirring at 0 ℃ for reaction for 30 minutes, slowly adding 4-methoxybenzyl chloride (1.0mL, 7.42mmol) in portions, removing the ice bath, heating to room temperature, stirring for reaction for 3 hours, adding 50mL of ice water to quench the reaction after the reaction is completed, extracting with ethyl acetate (20mL x 4), combining organic phases, washing an organic layer by 50mL of saturated saline, drying with anhydrous sodium sulfate, concentrating, separating by a silica gel column (2:1, V/V, petroleum ether: EtOAc) to obtain a product 2c (1.1g, 3.56mmol), wherein the yield: 72 percent.
High resolution mass spectrometry (ESI)+)：C17H23O6 +Theoretical value is 323.1489, found 323.1483.
Example 10
Preparation of compound 2 d:
dissolving the raw material 6b (1.0g,5.31mmol) in anhydrous DMF (20mL), cooling to 0 ℃ in an ice bath, slowly adding 60% NaH (0.3g, 7.97mmol) in portions under the protection of nitrogen, stirring at 0 ℃ for reaction for 30 minutes, slowly adding 4-methoxy benzyl chloride (1.1mL, 7.97mmol) in portions, removing the ice bath, heating to room temperature, stirring for reaction for 3 hours, adding 50mL of ice water to quench the reaction after the reaction is completed, extracting with ethyl acetate (20mL x 4), combining organic phases, washing an organic layer by 50mL of saturated saline water, drying with anhydrous sodium sulfate, concentrating, separating by a silica gel column (2:1, V/V, petroleum ether: EtOAc) to obtain a product 2d (1.2g, 3.99mmol), wherein the yield: 75 percent.
High resolution mass spectrometry (ESI)+)：C16H21O6 +Theoretical value is 309.1333, found 309.1328.
Example 11
Preparation of Compounds 2-1 c:
dissolving 6-1c (5.0g,30.84mmol) of raw material in anhydrous DMF (200mL), cooling to-10 ℃ under the protection of nitrogen, slowly adding 60% NaH (1.6g, 40.09mmol), stirring at-10 ℃ for reaction for 1 hour, slowly adding 2-bromomethylnaphthalene (10.2g, 46.26mmol), stirring for 30 minutes, adding 60% NaH (1.6g, 40.09mmol) and 2-bromomethylnaphthalene (10.2g, 46.26mmol) according to the above operation, repeating twice, stirring at-10 ℃ for reaction for 48 hours, pouring the reaction liquid into 500mL of ice water to quench the reaction, extracting with ethyl acetate (100mL x 4), combining organic phases, washing the organic layer with 200mL of saturated saline, drying with sodium sulfate, concentrating, separating with silica gel column (10:1, V/V, petroleum ether: EtOAc) to obtain colorless oily product 2-1c (12.6g, 21.59mmol), yield: 70 percent.
High resolution mass spectrometry (ESI)+)：C39H35O5 +Theoretical value is 583.2479, found 583.2471.
Example 12
Preparation of Compounds 2-1 d:
dissolving 6-1d (5.0g,33.76mmol) of raw material in anhydrous DMF (200mL), cooling to-10 deg.C under the protection of nitrogen, slowly adding 60% NaH (1.8g, 43.89mmol), stirring at-10 deg.C for 1 hr, slowly adding 2-bromomethylnaphthalene (11.2g, 50.64mmol), stirring for 30 min, adding 60% NaH (1.8g, 43.89mmol) and 2-bromomethylnaphthalene (11.2g, 50.64mmol), repeating twice, stirring at-10 deg.C for 48 hr, pouring the reaction solution into 500mL of ice water to quench the reaction, extracting with ethyl acetate (100 mL. times.4), combining the organic phase, washing the organic layer with 200mL of saturated saline, drying over anhydrous sodium sulfate, concentrating, separating with silica gel column (10:1, V/V, petroleum ether: EtOAc) to obtain white oily product 2-1d (13.8g, 24.31mmol), yield: 72 percent.
High resolution mass spectrometry (ESI)+)：C38H33O5 +Theoretical value is 569.2323, found 569.2315.
Example 13
Preparation of Compound 8-1 a:
the method comprises the following steps: cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2a (656mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), brought to room temperature, extracted 4 times with ethyl acetate (10 mL. times.4), and combinedThe organic layer was washed with a saturated sodium bicarbonate solution and a saturated sodium chloride solution, dried over anhydrous sodium sulfate, and concentrated to give crude product 7-1 a. High resolution mass spectrometry (ESI)+)：C31H37N4O7 +Theoretical value is 577.2657, found 577.2651.
The crude 7-1a obtained above was dissolved in 10mL of dry MeCN under nitrogen protection, cooled to-40 ℃ and Et was added3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), reaction at-40 ℃ for 2h, TLC detection, quenching with saturated aqueous sodium bicarbonate (5mL), extraction of the aqueous layer with dichloromethane (10 mL. times.3) for 3 times, combining the organic layers, washing with saturated brine, drying over anhydrous sodium sulfate, concentration, and purification by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give the product 8-1a (183mg,0.33mmol) as a white amorphous solid in yield: 51 percent.
Rf0.71(1:1, V/V, petroleum ether: EtOAc); [ alpha ] to]D 20＝-100.48(c＝0.210,CHCl3)；HPLC tR＝3.74 min；1H-NMR(500MHz,CDCl3)δ8.16(s,1H,ArH),7.85–7.82(m,4H,ArH),7.54–7.47 (m,3H,ArH),7.21(d,J＝4.2Hz,1H,ArH),7.05(d,J＝3.7Hz,1H,ArH),5.86(s,1H,H-1), 4.80(d,J＝12.3Hz,1H,OCH2-),4.75(d,J＝12.2Hz,1H,OCH2-),4.64(s,1H,H-3),4.40(t, J＝4.1Hz,1H,H-4),3.79–3.76(m,2H,H-5,H-5’),1.59(s,3H,2-CH3),1.57(s,9H, C(CH3)3),1.47(s,3H,OC(CH3)2O),1.38(s,3H,OC(CH3)2O), NH is absent; selected fractions NOESY (500MHz, CDCl)3):δ(1H)/δ(1H)＝5.86/3.79–3.76(H-1/H-5,H-5’)；13C-NMR (126MHz,CDCl3)δ151.24(C＝O),150.25(ArC),146.05(ArC),135.40(ArC),133.39(ArC), 133.15(ArC),129.26(ArC),128.37(ArC),127.98(ArC),127.83(ArC),126.64(ArC), 126.28(ArC),126.08(ArC),125.76(ArC),115.10(ArC),114.08(ArC),113.06 (OC(CH3)2O),105.22(ArC),91.13(C-2),89.49(C-3),83.31(C-4),82.67(C(CH3)3),80.48 (C-1),73.93(OCH2-),71.41(C-5),28.27(C(CH3)3),27.76(OC(CH3)2O),27.52 (OC(CH3)2O),23.08(2-CH3) (ii) a High resolution mass spectrometry (ESI)+)：C31H37N4O6 +The theoretical value was 561.2708, and the measured value was 561.2701.
The second method comprises the following steps: cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2a (656mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), returned to room temperature, extracted 4 times with ethyl acetate (10mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-1 a. High resolution mass spectrometry (ESI)+)：C31H37N4O7 +Theoretical value is 577.2657, found 577.2651.
The crude product 7-1a obtained above was dissolved in 10mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (408. mu.L, 2.56mmol), stirring for 5min, adding TMSOTf (247. mu.L, 1.28mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (5mL), quenching, and adding CH to water layer2Cl2(10 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give the product 8-1a (269mg,0.48mmol) as a white amorphous solid in yield: 75 percent.
The analytical data were consistent with 8-1a obtained in method one.
Example 14
Preparation of Compound 8-1 b:
cooling 5-1a (0.5g, 1.60mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.04mL, 2.08mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C for 50min, cooling to-78 deg.C, adding n-BuLi (2.5mL, 3.99mmol, 1.6M hexane solution dropwise), stirring for 5min, adding 2c (1.5g, 4.79mmol) THF (20mL), maintaining at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (40mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (20mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-1 b. High resolution mass spectrometry (ESI)+): theoretical value of C28H37N4O8 +557.2606, found 557.2659.
The crude product 7-1b obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (1.02mL,6.39mmol), stirring for 5min, adding TMSOTf (0.6mL,3.19mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (20mL), quenching, and adding CH to the water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (1:1, V/V, petroleum ether: EtOAc) to give the product 8-1b (518mg,0.96mmol) as a white amorphous solid in yield: 60 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak delta 5.7(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C28H37N4O7 +Theoretical value is 541.2657, found 541.2648.
Example 15
Preparation of Compound 8-1 a:
cooling 5-1b (0.2g, 0.56mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.36mL, 0.72mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (0.87mL, 1.39mmol, 1.6M hexane solution), stirring for 5min, adding 2a (570mg, 1.67mmol) THF (10mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (20mL), returned to room temperature, and then extracted 4 times with ethyl acetate (20mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-1 a. High resolution mass spectrometry (ESI)+)：C31H37N4O7 +Theoretical value is 577.2657, found 577.2651.
The crude product 7-1a obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.35mL,2.22mmol), stirring for 5min, adding TMSOTf (0.21mL,1.11mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (2:1, V/V, petroleum ether: EtOAc) to give the product 8-1a (249mg,0.44mmol) as a white amorphous solid in yield: 80 percent.
The analytical data agree with the product 8-1a of example 13.
Example 16
Preparation of compound 8 c:
under the anhydrous and anaerobic condition, the mixture is prepared5-1c (0.5g, 1.48mmol) of THF (20mL) solution is cooled to-30 ℃, diisopropylaminolithium THF solution (0.96mL, 1.92mmol, 2M tetrahydrofuran solution) is added dropwise, the temperature is controlled at-30 ℃, after stirring reaction for 50min, the temperature is continuously reduced to-78 ℃, n-BuLi (2.3mL, 3.70mmol, 1.6M hexane solution) is added dropwise, after the dropwise addition, the stirring reaction is carried out for 5min, 2a (1.52g, 4.44mmol) of THF (20mL) solution is added dropwise, the temperature is kept at-78 ℃, the reaction is carried out for 2h, after TLC detection reaction, saturated NH is added4The reaction was quenched with aqueous Cl (40mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (30mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7 c. High resolution mass spectrometry (ESI)+): theoretical value of C32H36N5O7 +602.2609, found 602.2604.
The crude product 7c obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.94mL,5.91mmol), stirring for 5min, adding TMSOTf (0.57mL,2.96mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (2:1, V/V, petroleum ether: EtOAc) to give product 8c (554mg,0.95mmol) as a white amorphous solid in yield: and 64 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak delta 5.4(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C32H36N5O6 +Theoretical value is 586.2660, found 586.2652.
Example 17
Preparation of compound 8 d:
under the anhydrous and oxygen-free conditions, 5-1d (0) is added.5g, 1.47mmol) of THF (20mL) to-30 deg.C, dropwise adding lithium diisopropylamide THF solution (0.96mL, 1.92mmol, 2M tetrahydrofuran solution), controlling the temperature at-30 deg.C, stirring for 50min, further cooling to-78 deg.C, dropwise adding n-BuLi (2.3mL, 3.69mmol, 1.6M hexane solution), stirring for 5min after dropwise addition, dropwise adding 2a (1.5g, 4.42mmol) of THF (20mL), maintaining the temperature at-78 deg.C, reacting for 2 hr, and after TLC detection, adding saturated NH4The reaction was quenched with aqueous Cl (40mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (30mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude product 7 d. High resolution mass spectrometry (ESI)+): theoretical value of C33H39N4O7 +603.2813, found 603.2808.
The crude product 7d obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.94mL,5.90mmol), stirring for 5min, adding TMSOTf (0.57mL,2.95mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (2:1, V/V, petroleum ether: EtOAc) to give the product 8d (519mg,0.88mmol) as a white amorphous solid in yield: 60 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak delta 5.9(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C33H39N4O6 +Theoretical value is 587.2864, found 587.2859.
Example 18
Preparation of Compounds 8-1 e:
under anhydrous and oxygen-free conditions, 5-1a (200mg,0.64mmol) of THF (5mL) to-30 deg.C, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling the temperature at-30 deg.C, stirring for reaction for 50min, cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution) dropwise, stirring for reaction for 5min, adding 2b (629mg, 1.92mmol) of THF (10mL), keeping the temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (10mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-1 e. High resolution mass spectrometry (ESI)+)：C30H35N4O7 +Theoretical value is 563.2500, found 563.2488.
The crude 7-1e from above was dissolved in 10mL dry CH under nitrogen2Cl2In (b), cooled to-78 ℃, Et is added3SiH (408. mu.L, 2.56mmol), stirring for 5min, adding TMSOTf (247. mu.L, 1.28mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (5mL), quenching, and adding CH to water layer2Cl2(10 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give the product 8-1e (287mg,0.52mmol) as a white amorphous solid in yield: 82 percent.
Rf0.46(2:1, V/V, petroleum ether: EtOAc); [ alpha ] to]D 20＝-131.30(c＝0.115,CHCl3)；HPLC tR＝3.62 min；1H-NMR(500MHz,CDCl3)δ8.16(s,1H,ArH),7.85–7.81(m,4H,ArH),7.50–7.45 (m,3H,ArH),7.19(d,J＝3.6Hz,1H,ArH),7.02(s,1H,ArH),5.89(s,1H,H-1),5.11(s,1H, H-2),4.96(d,J＝5.9Hz,1H,H-3),4.78(d,J＝12.3Hz,1H,OCH2-),4.72(d,J＝12.3Hz, 1H,OCH2-),4.42(t,J＝3.5Hz,1H,H-4),3.75(dd,J＝10.2,3.8Hz,1H,H-5),3.71(dd,J＝ 10.2,4.2Hz,1H,H-5’),1.56(s,9H,C(CH3)3),1.40(s,3H,OC(CH3)2O),1.28(s,3H, OC(CH3)2O), NH is absent; selected fractions NOESY (500MHz, CDCl)3):δ(1H)/δ(1H)＝5.89/ 3.75,3.71(H-1/H-5,H-5’)；13C-NMR(126MHz,CDCl3)δ151.08(C＝O),150.20(ArC), 146.26(ArC),135.42(ArC),133.40(ArC),133.16(ArC),129.05(ArC),128.42(ArC), 128.01(ArC),127.84(ArC),126.57(ArC),126.31(ArC),126.10(ArC),125.67(ArC), 115.00(ArC),113.33(ArC),112.79(OC(CH3)2O),105.03(ArC),83.58(C-3),82.97(C-4), 82.71(C(CH3)3),81.57(C-2),77.25(C-1),73.88(OCH2-),71.44(C-5),28.28(C(CH3)3), 26.38(OC(CH3)2O),25.18(OC(CH3)2O); high resolution mass spectrometry (ESI)+)：C30H35N4O6 +Theoretical value is 547.2551, found 547.2565.
Example 19
Cooling 5-1a (0.5g, 1.60mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.04mL, 2.08mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (2.5mL, 3.99mmol, 1.6M hexane solution dropwise), stirring for 5min, adding 2d (1.57g, 4.79mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4Quenching the reaction with a Cl aqueous solution (40mL), returning to room temperature, extracting with ethyl acetate (20mL × 4) for 4 times, combining the organic layers, washing with saturated sodium bicarbonate solution and saturated salt water, drying with anhydrous sodium sulfate, and concentrating to obtain crude product 7-1 f; high resolution mass spectrometry (ESI)+): theoretical value of C27H35N4O8 +543.2449, found 543.2595.
Under the protection of nitrogen, the crude product 7-1f obtained above is dissolved in 20mL of dryCH (A) of2Cl2In (b), cooled to-78 ℃, Et is added3SiH (1.02mL,6.39mmol), stirring for 5min, adding TMSOTf (0.6mL,3.19mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (20mL), quenching, and adding CH to the water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (1:1, V/V, petroleum ether: EtOAc) to give the product 8-1f (589mg,1.12mmol) as a white amorphous solid in yield: 70 percent.
High resolution mass spectrometry (ESI)+)：C27H35N4O7 +Theoretical value is 527.2500, found 527.2492.
Example 20
Preparation of compound 8 g:
cooling 5-1c (0.5g, 1.48mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.96mL, 1.92mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (2.3mL, 3.70mmol, 1.6M hexane solution), stirring for 5min, adding 2b (1.46g, 4.44mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4Quenching the reaction with a Cl aqueous solution (40mL), returning to room temperature, extracting with ethyl acetate (30 mL. times.4) for 4 times, combining the organic layers, washing with saturated sodium bicarbonate solution and saturated salt water, drying with anhydrous sodium sulfate, and concentrating to obtain crude product 7 g; high resolution mass spectrometry (ESI)+)：C31H34N5O7 +Theoretical value is 588.2453, found 588.2538.
Under the protection of nitrogen, 7g of the crude product obtained above is dissolved in 20mL of dry CH2Cl2In (b), cooled to-78 ℃, Et is added3SiH(0.94mL,5.91mmol)Stirring for 5min, adding TMSOTf (0.57mL,2.96mmol) dropwise, reacting at-78 deg.C for 2h, detecting by TLC, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried with anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (2:1, V/V, petroleum ether: EtOAc) to give 8g (609mg,1.06mmol) of a white amorphous solid product, yield: 72 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak δ 5.4(d, J ═ 4.0Hz,1H, H-1); high resolution mass spectrometry (ESI)+)：C31H34N5O6 +Theoretical value is 572.2504, found 572.2493.
Example 21
Preparation of compound 8 h:
cooling 5-1d (0.5g, 1.47mmol) of THF (20mL) under anhydrous and oxygen-free conditions to-30 deg.C, adding diisopropylamino lithium THF solution (0.96mL, 1.92mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C for 50min, cooling to-78 deg.C, adding n-BuLi (2.3mL, 3.69mmol, 1.6M hexane solution dropwise), stirring for 5min, adding 2b (1.45g, 4.42mmol) of THF (20mL), maintaining at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4Quenching the reaction with a Cl aqueous solution (40mL), returning to room temperature, extracting with ethyl acetate (30mL × 4) for 4 times, combining the organic layers, washing with saturated sodium bicarbonate solution and saturated salt water, drying with anhydrous sodium sulfate, and concentrating to obtain a crude product for 7 h; high resolution mass spectrometry (ESI)+)：C32H37N4O7 +Theoretical value is 589.2657, found 589.2701. The crude product obtained above is dissolved for 7h in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.94mL,5.90mmol), stirred for 5min, and TMSOTf (R) (b)0.57mL,2.95mmol), reacting at-78 deg.C for 2h, detecting by TLC, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with brine, dried over anhydrous sodium sulfate, concentrated, and isolated and purified on silica gel (2:1, V/V, petroleum ether: EtOAc) to give the product as a white amorphous solid for 8h (540mg,0.94 mmol), yield: and 64 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak δ 5.9(d, J ═ 4.0Hz,1H, H-1); high resolution mass spectrometry (ESI)+)：C32H37N4O6 +Theoretical value is 573.2708, found 573.2856.
Example 22
Preparation of Compounds 8-1 e:
cooling 5-1b (0.2g, 0.56mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.36mL, 0.72mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (0.87mL, 1.39mmol, 1.6M hexane solution), stirring for 5min, adding 2b (547mg, 1.67mmol) THF (10mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4Quenching the reaction with aqueous Cl (20mL), returning to room temperature, extracting with ethyl acetate (20 mL. times.4) for 4 times, combining the organic layers, washing with saturated sodium bicarbonate solution and saturated brine, drying over anhydrous sodium sulfate, and concentrating to obtain crude product 7-1 e; high resolution mass spectrometry (ESI)+)：C30H35N4O7 +Theoretical value is 563.2500, found 563.2488.
The crude 7-1e from above was dissolved in 20mL dry CH under nitrogen2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.35mL,2.22mmol), stirred for 5min, and then added dropwiseAdding TMSOTf (0.21mL,1.11mmol), reacting at-78 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (20mL), quenching the reaction, and adding CH to the water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (2:1, V/V, petroleum ether: EtOAc) to give the product 8-1e (219mg,0.40mmol) as a white amorphous solid in yield: 72 percent.
The analytical data agree with the products 8-1e of example 18.
Example 23
Preparation of compound 8 i:
cooling 5-2a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2a (656mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4Quenching the reaction with a Cl aqueous solution (10mL), returning to room temperature, extracting with ethyl acetate (10 mL. times.4) for 4 times, combining the organic layers, washing with saturated sodium bicarbonate solution and saturated salt water, drying over anhydrous sodium sulfate, and concentrating to obtain crude product 7-2 i; high resolution mass spectrometry (ESI)+)：C30H36N5O7 +Theoretical value is 578.2609, found 578.2600.
Dissolving the crude product 7-2i obtained above in 10mL dry MeCN under nitrogen protection, cooling to-40 deg.C, adding Et3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection, quenched with saturated aqueous sodium bicarbonate (10mL), the aqueous layer extracted 3 times with dichloromethane (10 mL. times.3), and combinedAnd the organic layer was washed with brine, dried over anhydrous sodium sulfate, concentrated and purified by silica gel column separation (25:1, V/V, DCM: MeOH) to give the product 8-2i (162mg,0.35mmol) as a white amorphous solid in yield: and 55 percent.
Rf＝0.63(15:1，V/V，DCM:MeOH)；[α]D 20＝-92.00(c＝0.125,CHCl3)；HPLC tR＝3.36 min；1H-NMR(500MHz,CDCl3)δ8.10(s,1H,ArH),7.87–7.81(m,4H,ArH),7.77(s,1H, ArH),7.56–7.51(m,1H,ArH),7.50–7.45(m,2H,ArH),5.68(s,1H,H-1),4.80(d,J＝ 12.1Hz,1H,OCH2-),4.75(d,J＝12.1Hz,1H,OCH2-),4.67(s,1H,H-3),4.37(t,J＝3.8Hz, 1H,H-4),3.80(dd,J＝10.3,3.8Hz,1H,H-5),3.76(dd,J＝10.3,4.1Hz,1H,H-5’),1.57(s, 3H,2-CH3),1.50(s,3H,OC(CH3)2O),1.40(s,3H,OC(CH3)2O)，NH2Removing the defect; selected fractions NOESY (500MHz, CDCl)3):δ(1H)/δ(1H)＝5.68/3.80,3.76(H-1/H-5,H-5’)；13C-NMR (126MHz,CDCl3)δ153.84(ArC),148.80(ArC),135.34(ArC),133.41(ArC),133.19(ArC), 132.74(ArC),128.48(ArC),128.44(ArC),128.01(ArC),127.86(ArC),126.97(ArC), 126.73(ArC),126.32(ArC),126.13(ArC),125.80(ArC),113.24(OC(CH3)2O),90.84(C-2), 89.47(C-3),83.54(C-4),79.74(C-1),74.01(OCH2-),71.60(C-5),27.90(OC(CH3)2O), 27.62(OC(CH3)2O),22.77(2-CH3) (ii) a High resolution mass spectrometry (ESI)+)：C25H28N5O4 +Theoretical value is 462.2136, found 462.2133.
Example 24
Preparation of Compounds 8-2 j:
a solution of 5-2a (0.5g, 1.59mmol) in THF (10mL) was cooled to-30 deg.C under anhydrous and oxygen-free conditions, and lithium diisopropylamide THF solution (1.03mL, 2.07mmol, 2M tetra-N-ethyl-N-isopropylamide) was added dropwiseTetrahydrofuran solution), controlling the temperature at-30 ℃, stirring for reaction for 50min, then continuously cooling to-78 ℃, dropwise adding n-BuLi (2.5mL, 3.98mmol, 1.6M hexane solution), stirring for reaction for 5min after dropwise adding, dropwise adding 2c (1.54g, 4.77mmol) THF (20mL), keeping the temperature at-78 ℃, reacting for 2h, after TLC detection reaction, adding saturated NH4The reaction was quenched with aqueous Cl (40mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (20mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-2 j. High resolution mass spectrometry (ESI)+)：C27H36N5O8 +Theoretical value is 558.2558, found 558.2550.
Dissolving the crude product 7-2j obtained above in 10mL of dried MeCN under nitrogen protection, cooling to-40 deg.C, adding Et3SiH (1.5mL,9.55mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.4mL,3.18mmol), naturally heating to room temperature, reacting for 2h, after TLC detection reaction is finished, adding saturated sodium bicarbonate aqueous solution (10mL) to quench the reaction, extracting the water layer with ethyl acetate (10mL × 3) for 3 times, combining the organic layers, washing with saturated common salt water, drying with anhydrous sodium sulfate, concentrating, and separating and purifying with silica gel column (25:1, V/V, DCM: MeOH) to obtain white amorphous solid product 8-2j (386mg,0.87mmol), yield: and 55 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak delta 5.6(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C22H28N5O5 +Theoretical value is 442.2085, found 442.2077.
Example 25
Preparation of Compound 8-2 k:
a solution of 5-2b (0.2g, 0.55mmol) in THF (10mL) was cooled to-30 deg.C under anhydrous and oxygen-free conditions, and lithium diisopropylamide THF solution (0.36mL, 0.72mmol, 2M in tetrahydrofuran) was added dropwiseControlling the temperature at-30 ℃, stirring for reaction for 50min, then continuously cooling to-78 ℃, dropwise adding n-BuLi (0.87mL, 1.38mmol and 1.6M hexane solution), stirring for reaction for 5min after dropwise adding, dropwise adding 2a (569mg and 1.66mmol) THF (10mL) solution, keeping the temperature at-78 ℃, reacting for 2h, and adding saturated NH after TLC detection reaction4The reaction was quenched with aqueous Cl (20mL), returned to room temperature, and then extracted 4 times with ethyl acetate (20mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-2 i. High resolution mass spectrometry (ESI)+)：C30H36N5O7 +Theoretical value is 578.2609, found 578.2600.
Dissolving the crude product 7-2i obtained above in 10mL dry MeCN under nitrogen protection, cooling to-40 deg.C, adding Et3SiH (0.35mL,2.22mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.14mL,1.11mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection reaction, added saturated sodium bicarbonate aqueous solution (10mL) to quench the reaction, the aqueous layer was extracted with ethyl acetate (10 mL. times.3) for 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, DCM: MeOH) to give 8-2i (133mg,0.29mmol) as a white amorphous solid product, yield: 52 percent.
The analytical data agree with the products 8-2i of example 23.
Example 26
Preparation of Compound 8-2 k:
cooling 5-2a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2b (629mg, 1.92mmol) THF dropwise(10mL) solution, keeping the temperature at-78 ℃, reacting for 2 hours, adding saturated NH after TLC detection reaction4The reaction was quenched with aqueous Cl (10mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (10mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-2 k. High resolution mass spectrometry (ESI)+)：C29H34N5O7 +Theoretical value is 564.2453, found 564.2446.
Dissolving the crude product 7-2k in 10mL dry MeCN under nitrogen, cooling to-40 deg.C, adding Et3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection reaction was completed, the reaction was quenched with saturated aqueous sodium bicarbonate (10mL), the aqueous layer was extracted 3 times with dichloromethane (10 mL. times.3), the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, DCM: MeOH) to give 8-2k (155 mg,0.35mmol) as a white solid, yield: 54 percent.
Rf＝0.60(15:1，V/V，DCM:MeOH)；[α]D 20＝-62.14(c＝0.140,CHCl3)；HPLC tR＝3.29min； 1H-NMR(600MHz,CDCl3)δ8.10(s,1H,ArH),7.83(dd,J＝15.5,8.4Hz,4H,ArH),7.74(s, 1H,ArH),7.51–7.45(m,3H,ArH),5.79(d,J＝4.1Hz,1H,H-1),5.07(dd,J＝5.8,4.2Hz, 1H,H-2),4.98(d,J＝6.0Hz,1H,H-3),4.78(d,J＝12.3Hz,1H,OCH2-),4.72(d,J＝12.3 Hz,1H,OCH2-),4.41(t,J＝3.7Hz,1H,H-4),3.76(dd,J＝10.3,3.6Hz,1H,H-5),3.71(dd, J＝10.3,4.0Hz,1H,H-5’),2.17(s,2H,NH2),1.45(s,3H,OC(CH3)2O),1.31(s,3H, OC(CH3)2O)；，NH2Removing the defect; selected fractions NOESY (500MHz, CDCl)3):δ(1H)/δ(1H)＝5.79/ 3.76,3.71(H-1/H-5,H-5’)；13C NMR(151MHz,CDCl3)δ153.85(ArC),148.84(ArC), 135.32(ArC),133.37(ArC),133.15(ArC),131.92(ArC),128.46(ArC),128.41(ArC), 127.99(ArC),127.83(ArC),126.75(ArC),126.63(ArC),126.32(ArC),126.13(ArC), 125.69(ArC),112.97(OC(CH3)2O),83.63(C-3),83.08(C-4),81.64(C-2),76.02(C-1),73.90 (OCH2-),71.54(C-5),26.39(OC(CH3)2O),25.13(OC(CH3)2O); high resolution mass spectrometry (ESI)+)： C24H25N5NaO4 +Theoretical value is 470.1799, found 470.1806.
Example 27
Preparation of Compound 8-2 l:
cooling 5-2a (0.5g, 1.59mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.03mL, 2.07mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (2.5mL, 3.98mmol, 1.6M hexane solution dropwise), stirring for 5min, adding 2d (1.47g, 4.77mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (40mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (20 mL. times.4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give 7-2l of crude product. High resolution mass spectrometry (ESI)+)：C26H34N5O8 +Theoretical value is 544.2402, found 544.2445.
Dissolving 7-2l of crude product obtained above in 20mL of dried MeCN under nitrogen protection, cooling to-40 deg.C, and adding Et3SiH (1.02mL,6.37mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.4mL,3.18mmol), naturally heating to room temperature, reacting for 2h, after TLC detection reaction, adding saturated sodium bicarbonate water solution (20mL) to quench reaction, extracting water layer with ethyl acetate (10 mL. times.3) for 3 times, combining organic layers, washing with saturated salt water, drying with anhydrous sodium sulfate, concentrating, and performing silica gel columnPurification by isolation (25:1, V/V, DCM: MeOH) afforded 8-2l (381mg,0.89mmol) of the product as a white amorphous solid in yield: 56 percent.
1H-NMR(500MHz,CDCl3) alpha-configuration characteristic peak δ 5.7(d, J ═ 4.0Hz,1H, H-1); high resolution mass spectrometry (ESI)+)：C21H26N5O5 +Theoretical value is 428.1928, found 428.1922.
Example 28
Preparation of Compound 8-2 k:
cooling 5-2b (0.2g, 0.55mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.36mL, 0.72mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (0.87mL, 1.38mmol, 1.6M hexane solution), stirring for 5min, adding 2b (546mg, 1.66mmol) THF (10mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (20mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (20mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 7-2 k.
Dissolving the crude product 7-2k in 10mL dry MeCN under nitrogen, cooling to-40 deg.C, adding Et3SiH (0.35mL,2.22mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.14mL,1.11mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection reaction, added saturated sodium bicarbonate aqueous solution (10mL) to quench the reaction, the aqueous layer was extracted with ethyl acetate (10 mL. times.3) for 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, DCM: MeOH) to give 8-2k (149mg,0.33mmol) as a white amorphous solid product, yield: 60 percent.
The analytical data agree with the products 8-2k of example 26.
Example 29
Preparation of compound 3 c:
the method comprises the following steps: cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1a (830mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), returned to room temperature, and then extracted 4 times with ethyl acetate (10mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9 a. High resolution mass spectrometry (ESI)+)：C38H43N4O7 +Theoretical value is 667.3126, found 667.3121.
The crude product 9a obtained above was dissolved in 10mL of dry CH under nitrogen protection2Cl2In (b), cooling to-10 ℃, and adding Et3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), reacting at-10 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (5mL) to quench the reaction, and adding CH to the water layer2Cl2(10 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with brine, dried over anhydrous sodium sulfate, concentrated, and isolated and purified by silica gel column (4:1, V/V, petroleum ether: EtOAc) to give product 3c (337mg,0.52mmol) as a white amorphous solid in yield: 81 percent.
Rf0.44(4:1,60-90 ℃ petroleum ether-EtOAc); [ alpha ] to]D 20＝24.52(c＝0.155，CHCl3)；HPLC tR＝4.10min；1H NMR(600MHz,CDCl3)δ8.15(s,2H,ArH,NH),7.43–7.25(m,15H, Bn-H),7.10(dd,J＝21.6,4.0Hz,2H,ArH),5.94(s,1H,H-1),4.86(dd,J＝55.4,12.0Hz, 2H,Bn-CH2-),4.69–4.57(m,4H,2x Bn-CH2-),4.42(d,J＝8.3Hz,1H,H-4),4.06(d,J＝ 8.7Hz,1H,H-3),3.91(d,J＝10.5Hz,1H,H-5),3.72(dd,J＝10.9,3.2Hz,1H,H-5’),1.57 (s,9H,C(CH3)3),1.01(s,3H,2-CH3) (ii) a Selected fractions NOESY (600MHz, CDCl)3):δ(1H)/δ (1H)＝7.10/3.91,3.72,4.06(ArH/H-5,H-5’,H-3)；13C NMR(151MHz,CDCl3)δ151.20 (ArC),150.35(C＝O),146.02(ArC),139.76(Bn-C),138.30(Bn-C),138.26(Bn-C),132.14 (ArC),128.55(2x Bn-C),128.42(2x Bn-C),128.32(3x Bn-C),127.97(2x Bn-C),127.95 (2x Bn-C),127.81(Bn-C),127.26(2x Bn-C),127.21(Bn-C),115.10(ArC),112.41(ArC), 105.63(ArC),85.18(C-2),82.81(C(CH3)3),82.17(C-3),79.39(C-4),77.97(C-1),73.59(2x Bn-CH2-),69.17(C-5),65.91(Bn-CH2-),28.26(C(CH3)3),17.47(2-CH3). High resolution mass spectrometry (ESI)+)：C38H42N4NaO6 +Theoretical value is 673.2997, found 673.3004.
The second method comprises the following steps: cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1a (830mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), returned to room temperature, and then extracted 4 times with ethyl acetate (10mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9 a. High resolution mass spectrometry (ESI)+)：C38H43N4O7 +Theoretical value of 667.3126, found value of 667.3121。
The crude product 9a obtained above was dissolved in 10mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (408. mu.L, 2.56mmol), stirring for 5min, adding TMSOTf (247. mu.L, 1.28mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (5mL), quenching, and adding CH to water layer2Cl2(10 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give product 3c (333mg,0.51mmol) as a white amorphous solid in yield: 80 percent.
The analytical data were consistent with 3c obtained in method one.
Example 30
Preparation of compound 3 a:
cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1a (830mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), returned to room temperature, and then extracted 4 times with ethyl acetate (10mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9 a. High resolution mass spectrometry (ESI)+)：C38H43N4O7 +Theoretical value is 667.3126, found 667.3120.
The crude product 9a obtained above was dissolved in 10mL of dry CH under nitrogen protection2Cl2In (1), cooling to 0 ℃ and adding Et3SiH (408. mu.L, 2.56mmol), stirring 5After min, BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), returning to room temperature after completion of dropwise addition reaction for 1h, adding saturated sodium bicarbonate aqueous solution (5mL) to quench the reaction after completion of TLC detection reaction, and adding CH to the aqueous layer2Cl2(10 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give product 3a (229mg,0.42mmol) as a white amorphous solid in yield: 65 percent.
1H-NMR(400MHz,CDCl3)δ7.89(s,1H),7.51–7.20(m,15H),6.90(d,J＝4.5
Hz,1H),6.53(d,J＝4.5Hz,1H),5.91(s,1H),5.65(br s,2H),4.92(d,J＝12.1Hz,1H),4.81 (d,J＝12.1Hz,1H),4.76–4.54(m,4H),4.41(dt,J＝8.4,2.4Hz,1H),4.08(d,J＝8.7Hz,
1H) 3.91(dd, J ═ 10.9,2.4Hz,1H),3.72(dd, J ═ 10.3,4.2Hz,1H),1.03(s, 3H). Data were analyzed against literature [ yarlagada, s.babu; pooran, Chand; pravin, Kotian; minwan, Wu; v. Satish, Kumar, Antiviral therapeutic agents, U.S. 20090227524A1, 2009).
High resolution mass spectrometry (ESI)+)：C33H35N4O4 +Theoretical value is 551.2653, found 551.2645.
Example 31
Preparation of Compounds 3-3 d:
cooling 5-1a (1.0g, 3.19mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (2.1mL, 4.15mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (5.0mL, 7.98mmol, 1.6M hexane solution), stirring for 5min, adding 2-1c (5.6g, 9.58mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, and adding saturated NH4Aqueous Cl solution (50mL)After quenching the reaction and returning to room temperature, extraction was performed 4 times with ethyl acetate (40 mL. times.4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 b. High resolution mass spectrometry (ESI)+)：C50H49N4O7 +Theoretical value is 817.3596, found 817.3592.
The crude product 9-3b obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (2.0mL,12.77mmol), stirring for 5min, adding TMSOTf (1.2mL,6.39mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (40mL), quenching, and adding CH to the water layer2Cl2(15 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give the product 3-3d (1.9g,2.40mmol) as a white amorphous solid in yield: 75 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 6.0(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C50H49N4O6 +Theoretical value is 801.3647, found 801.3643.
Example 32
Preparation of Compounds 3-3 e:
cooling 5-1a (1.0g, 3.19mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (2.1mL, 4.15mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (5.0mL, 7.98mmol, 1.6M hexane solution), stirring for 5min, adding 2-1c (5.6g, 9.58mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, and adding saturated NH4Aqueous Cl solution (50mL)After the reaction was quenched and returned to room temperature, the mixture was extracted with ethyl acetate (40 mL. times.4) 4 times, and the organic layers were combined, washed with a saturated sodium bicarbonate solution and a saturated sodium chloride solution, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 b. High resolution mass spectrometry (ESI)+)：C50H49N4O7 +Theoretical value is 817.3596, found 817.3591.
The crude product 9-3b obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (1), cooling to 0 ℃ and adding Et3SiH (2.0mL,12.77mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.8mL,6.39mmol), returning to room temperature after dropwise addition for 1h, adding saturated sodium bicarbonate aqueous solution (40mL) to quench the reaction after TLC detection, and adding CH to the water layer2Cl2(15 mL. times.3) extraction was performed 3 times, organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give the product 3-3e (1.5g,2.08mmol) as a white amorphous solid in yield: 65 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 5.9(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C45H41N4O4 +Theoretical value is 701.3122, found 701.3117.
Example 33
Preparation of compound 3 c:
cooling 5-1b (1.0g, 2.78mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.8mL, 3.61mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction at-30 deg.C for 50min, further cooling to-78 deg.C, adding n-BuLi (4.3mL, 6.94mmol, 1.6M hexane solution dropwise), stirring for reaction for 5min, adding 2-1a (3.6g, 8.33mmol) THF (20mL) solution dropwise, maintaining temperature at-78 deg.C, reacting for 2 hr, TLC detecting reaction, adding saturated and saturated THF (20mL)NH4The reaction was quenched with aqueous Cl (50mL), allowed to return to room temperature, and then extracted 4 times with ethyl acetate (40mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude product 9 a. High resolution mass spectrometry (ESI)+)：C38H43N4O7 +Theoretical value is 667.3126, found 667.3118.
The crude product 9a obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (1.8mL,11.11mmol), stirring for 5min, adding TMSOTf (1.1mL,5.55mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (40mL), quenching, and adding CH to the water layer2Cl2(15 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give product 3c (1.3g,1.94mmol) as a white amorphous solid in yield: 70 percent.
The analytical data agree with product 3c from example 29.
Example 34
Preparation of compound 3 a:
cooling 5-1b (1.0g, 2.78mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.8mL, 3.61mmol, 2M tetrahydrofuran solution) dropwise, controlling the temperature at-30 deg.C, stirring for reaction at-30 deg.C for 50min, further cooling to-78 deg.C, adding n-BuLi (4.3mL, 6.94mmol, 1.6M hexane solution) dropwise, stirring for reaction for 5min, adding 2-1a (3.6g, 8.33mmol) THF (20mL) solution dropwise, maintaining the temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, and adding saturated NH4The reaction was quenched with an aqueous solution of Cl (50mL), and after returning to room temperature, it was extracted 4 times with ethyl acetate (40 mL. times.4), the organic layers were combined, washed with a saturated sodium bicarbonate solution and a saturated sodium chloride solution, dried over anhydrous sodium sulfate,concentration gave crude 9 a. High resolution mass spectrometry (ESI)+)：C38H43N4O7 +Theoretical value is 667.3126, found 667.3121.
The crude product 9a obtained above was dissolved in 20mL of dry CH under nitrogen protection2Cl2In (1), cooling to 0 ℃ and adding Et3SiH (1.8mL,11.11mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.7mL,5.55mmol), returning to room temperature after dropwise addition for 1h, adding saturated sodium bicarbonate water solution (40mL) to quench the reaction after TLC detection, and adding CH to the water layer2Cl2(15 mL. times.3) extraction was performed 3 times, organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give product 3a (0.9g,1.72mmol) as a white amorphous solid in yield: 62 percent.
The analytical data agree with product 3a from example 30.
Example 35
Preparation of compound 3 f:
cooling 5-1c (0.5g, 1.48mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.96mL, 1.92mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (2.3mL, 3.70mmol, 1.6M hexane solution), stirring for 5min, adding 2-1a (1.9g, 4.44mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, and adding saturated NH4Quenching the reaction with a Cl aqueous solution (40mL), returning to room temperature, extracting with ethyl acetate (30 mL. times.4) for 4 times, combining the organic layers, washing with saturated sodium bicarbonate solution and saturated salt water, drying with anhydrous sodium sulfate, and concentrating to obtain crude product 9 c; high resolution mass spectrometry (ESI)+)：C39H42N5O7 +Theoretical value of 692.3079, found valueIs 692.3071.
The crude product 9c obtained above was dissolved in 20mL dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.94mL,5.91mmol), stirring for 5min, adding TMSOTf (0.57mL,2.96mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (2:1, V/V, petroleum ether: EtOAc) to give product 3f (699mg,1.04mmol) as a white amorphous solid in yield: 70 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 5.8(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C39H42N5O6 +Theoretical value is 676.3130, found 676.3121.
Example 36
Preparation of Compounds 3-3 g:
cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1b (803mg, 1.92mmol) THF (10mL) solution dropwise, maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), brought to room temperature, extracted 4 times with ethyl acetate (10mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 d. High resolution mass spectrometry (ESI)+)：C37H41N4O7 +Theoretical value of 653.2970, found value of 653.2964。
Under the protection of nitrogen, the crude product 9-3d obtained above is dissolved in 10mL of dry CH2Cl2In (b), cooling to-10 ℃, and adding Et3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), reacting at-10 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (5mL) to quench the reaction, and adding CH to the water layer2Cl2(10 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with brine, dried over anhydrous sodium sulfate, concentrated, and isolated and purified with silica gel column (4:1, V/V, petroleum ether: EtOAc) to give 3-3g (355mg,0.56mmol) of a white amorphous solid product, yield: 87 percent.
Rf0.32(4:1,60-90 ℃ petroleum ether-EtOAc); [ alpha ] to]D 20＝69.49(c＝0.495，CHCl3)；HPLC tR＝3.97min；1H NMR(400MHz,CDCl3)δ8.77(s,1H,NH),8.20(s,1H,ArH),7.33–7.25 (m,15H,Bn-H),7.15(d,J＝4.7Hz,1H,ArH),6.93–6.81(m,1H,ArH),5.74(d,J＝3.6 Hz，1H,H-1),4.73(t,J＝10.2Hz,2H,Bn-CH2-),4.62–4.51(m,3H,Bn-CH2-,Bn-CH2-), 4.43–4.39(m,2H,H-4,Bn-CH2-),4.23–4.20(m,1H,H-2),4.11(dd,J＝6.7,4.9Hz,1H, H-3),3.82–3.76(m,1H,H-5),3.66(dd,J＝10.8,3.9Hz,1H,H-5’),1.57(s,9H,C(CH3)3) (ii) a Selected fractions NOESY (500MHz, CDCl)3):δ(1H)/δ(1H)＝6.93–6.81/3.82–3.76,3.66 (ArH/H-5,H-5’)；13C NMR(126MHz,CDCl3)δ151.45(ArC),150.55(C＝O),145.98 (ArC),138.34(Bn-C),137.97(Bn-C),137.95(Bn-C),131.06(ArC),128.45(2x Bn-C), 128.42(2x Bn-C),128.37(2x Bn-C),128.22(2x Bn-C),127.96(2x Bn-C),127.83(2x Bn-C),127.71(2x Bn-C),127.67(Bn-C),115.71(ArC),112.24(ArC),106.04(ArC),82.76 (C(CH3)3),80.63(C-4),79.12(C-2),77.23(C-3),76.36(C-1),73.48(Bn-CH2-),72.13 (Bn-CH2-),71.70(Bn-CH2-),69.70(C-5),28.27(C(CH3)3). High resolution mass spectrometry (ESI)+)： C37H40N4NaO6 +Theoretical value is 659.2840, found 659.2854.
Example 37
Preparation of compound 3-3 h:
cooling 5-1a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1b (803mg, 1.92mmol) THF (10mL) solution dropwise, maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), brought to room temperature, extracted 4 times with ethyl acetate (10mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 d. High resolution mass spectrometry (ESI)+)：C37H41N4O7 +Theoretical value is 653.2970, found 653.2963.
Under the protection of nitrogen, the crude product 9-3d obtained above is dissolved in 20mL of dry CH2Cl2In (1), cooling to 0 ℃ and adding Et3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), returning to room temperature after completion of dropwise addition reaction for 1h, adding saturated sodium bicarbonate aqueous solution (5mL) to quench the reaction after completion of TLC detection reaction, and adding CH to the aqueous layer2Cl2(10 mL. times.3) extraction was performed 3 times, organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give the product as a white amorphous solid for 3-3h (209mg,0.39mmol), yield: 61 percent.
1H-NMR(600MHz,CDCl3)δ7.90(s,1H),7.34–7.24(m,15H),6.68(d,J＝4.5 Hz,1H),6.50(d,J＝4.5Hz,1H),5.72(br s,2H)5.68(d,J＝42Hz,1H),4.73(q, J ═ 12.3 Hz,2H),4.60(d, J ═ 12.0Hz,1H), 4.58-4.49 (m,2H),4.44(d, J ═ 11.9Hz,1H),4.40(dt, J ═ 6.9,3.8Hz,1H),4.27(t, J ═ 4.6Hz,1H),4.12(dd, J ═ 6.4,5.1Hz,1H),3.78(dd, J ═ 10.7, 3.4Hz,1H),3.66(dd, J ═ 10.7,4.1Hz, 1H). Data were analyzed against literature [ LEE, Jae Moon; CHOI, Min Jeong, Compounds and compositions for modulating histone methyl transferase activity, WO2014035140A2,2014.
Example 38
Preparation of Compounds 3-3 i:
cooling 5-1a (1.0g, 3.19mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (2.1mL, 4.15mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (5.0mL, 7.98mmol, 1.6M hexane solution), stirring for 5min, adding 2-1d (5.4g, 9.58mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (50mL), brought to room temperature, extracted 4 times with ethyl acetate (40mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 e. High resolution mass spectrometry (ESI)+)：C49H47N4O7 +Theoretical value is 803.3439, found 803.3432.
The crude 9-3e obtained above was dissolved in 20mL dry CH under nitrogen protection2Cl2In (b), cooled to-78 ℃, Et is added3SiH (2.0mL,12.77mmol), stirring for 5min, adding TMSOTf (1.2mL,6.39mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (20mL), quenching, and adding CH to the water layer2Cl2Extracting (15 mL. times.3) for 3 times, mixing organic layers, washing with saturated salt water, drying with anhydrous sodium sulfate, concentrating, and making into silica gel columnIsolation purification (4:1, V/V, petroleum ether: EtOAc) afforded the product 3-3i (1.9g,2.36mmol) as a white amorphous solid, in yield: 74 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 5.8(d, J ═ 4.0Hz,1H, H-1); high resolution mass spectrometry (ESI)+)：C49H47N4O6 +Theoretical value is 787.3490, found 787.3485.
Example 39
Preparation of Compounds 3-3 j:
cooling 5-1a (1.0g, 3.19mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (2.1mL, 4.15mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (5.0mL, 7.98mmol, 1.6M hexane solution), stirring for 5min, adding 2-1d (5.4g, 9.58mmol) THF (20mL), reacting for 2 hr at-78 deg.C, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (50mL), brought to room temperature, extracted 4 times with ethyl acetate (40mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 e. High resolution mass spectrometry (ESI)+)：C49H47N4O7 +Theoretical value is 803.3439, found 803.3432.
The crude 9-3e obtained above was dissolved in 20mL dry CH under nitrogen protection2Cl2In (1), cooling to 0 ℃ and adding Et3SiH (2.0mL,12.77mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.8mL,6.39mmol), returning to room temperature after dropwise addition for 1h, adding saturated sodium bicarbonate water solution (20mL) to quench the reaction after TLC detection, and adding CH to the water layer2Cl2(15 mL. times.3) was extracted 3 times, the organic layers were combined, washed with saturated brine,drying with anhydrous sodium sulfate, concentrating, and purifying with silica gel column (25:1, V/V, dichloromethane: methanol) to obtain white amorphous solid product 3-3j (1.3g,1.92mmol), yield: 60 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 5.7(d, J ═ 4.0Hz,1H, H-1); high resolution mass spectrometry (ESI)+)：C44H39N4O4 +Theoretical value is 687.2966, found 687.2961.
Example 40
Preparation of Compounds 3-3 g:
cooling 5-1b (1.0g, 2.78mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.8mL, 3.61mmol, 2M tetrahydrofuran solution) dropwise, controlling the temperature at-30 deg.C, stirring for reaction at-30 deg.C for 50min, further cooling to-78 deg.C, adding n-BuLi (4.3mL, 6.94mmol, 1.6M hexane solution dropwise), stirring for reaction for 5min, adding 2-1b (3.5g, 8.33mmol) THF (20mL) solution dropwise, maintaining the temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (20mL), allowed to return to room temperature, extracted 4 times with ethyl acetate (40mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude product 9-3 d. High resolution mass spectrometry (ESI)+)：C37H41N4O7 +Theoretical value is 653.2970, found 653.2964.
Under the protection of nitrogen, the crude product 9-3d obtained above is dissolved in 20mL of dry CH2Cl2In (b), cooled to-78 ℃, Et is added3SiH (1.8mL,11.11mmol), stirring for 5min, adding TMSOTf (1.1mL,5.55mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated aqueous sodium bicarbonate solution (40mL), quenching, and adding CH to the water layer2Cl2(15 mL. times.3) extraction was performed 3 times, and the organic layers were combined and saturatedWashing with brine, drying with anhydrous sodium sulfate, concentrating, separating and purifying with silica gel column (4:1, V/V, petroleum ether: EtOAc) to obtain 3-3g (1.1g,1.80mmol) of a white amorphous solid product, yield: 65 percent.
The analytical data agree with 3-3g for the product of example 36.
EXAMPLE 41
Preparation of compound 3-3 h:
cooling 5-1b (1.0g, 2.78mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.8mL, 3.61mmol, 2M tetrahydrofuran solution) dropwise, controlling the temperature at-30 deg.C, stirring for reaction at-30 deg.C for 50min, further cooling to-78 deg.C, adding n-BuLi (4.3mL, 6.94mmol, 1.6M hexane solution dropwise), stirring for reaction for 5min, adding 2-1b (3.5g, 8.33mmol) THF (20mL) solution dropwise, maintaining the temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (50mL), brought to room temperature, extracted 4 times with ethyl acetate (40mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-3 d. High resolution mass spectrometry (ESI)+)：C37H41N4O7 +Theoretical value is 653.2970, found 653.2965.
Under the protection of nitrogen, the crude product 9-3d obtained above is dissolved in 20mL of dry CH2Cl2In (1), cooling to 0 ℃ and adding Et3SiH (1.8mL,11.11mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.7mL,5.55mmol), returning to room temperature after dropwise addition for 1h, adding saturated sodium bicarbonate water solution (40mL) to quench the reaction after TLC detection, and adding CH to the water layer2Cl2(15 mL. times.3) extraction was performed 3 times, the organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give the product as a white amorphous solid for 3h (894mg,1.67 mmol)) Yield, yield: 60 percent.
The analytical data are in agreement with the products of example 37 from 3 to 3 h.
Example 42
Preparation of compound 3 k:
cooling 5-1c (0.5g, 1.48mmol) THF (20mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.96mL, 1.92mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (2.3mL, 3.70mmol, 1.6M hexane solution dropwise), stirring for 5min, adding 2-1b (1.9g, 4.44mmol) THF (20mL), maintaining at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4Quenching the reaction with a Cl aqueous solution (10mL), returning to room temperature, extracting with ethyl acetate (30 mL. times.3) for 3 times, combining the organic layers, drying with anhydrous sodium sulfate, and concentrating to obtain crude product 9 f; high resolution mass spectrometry (ESI)+)：C38H40N5O7 +Theoretical value is 678.2922, found 678.2916.
The crude 9f obtained above was dissolved in 20mL of dry CH under nitrogen2Cl2In (b), cooled to-78 ℃, Et is added3SiH (0.94mL,5.91mmol), stirring for 5min, adding TMSOTf (0.57mL,2.96mmol) dropwise, reacting at-78 deg.C for 2h, after TLC detection, adding saturated sodium bicarbonate water solution (20mL), quenching, and adding CH to water layer2Cl2(20 mL. times.3) extraction was performed 3 times, the organic layers were combined, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (4:1, V/V, petroleum ether: EtOAc) to give the product 3k (665mg,1.01 mmol) as a white amorphous solid in yield: 68 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak delta 5.7(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C38H40N5O6 +Theoretical value of 662.2973Found 662.2965.
Example 43
Preparation of Compounds 3-4 b:
cooling 5-2a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1a (830mg, 1.92mmol) THF (10mL), maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), returned to room temperature, and then extracted 4 times with ethyl acetate (10mL × 4), and the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give 9-4g of crude product. High resolution mass spectrometry (ESI)+)：C37H42N5O7 +Theoretical value is 668.3079, found 668.3072.
Dissolving 9-4g of crude product obtained above in 10mL of dried MeCN under nitrogen protection, cooling to-40 deg.C, and adding Et3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection reaction, quenched with saturated aqueous sodium bicarbonate (10mL), the aqueous layer extracted 3 times with dichloromethane (10 mL. times.3), combined organic layers, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give the product 3-4b (212mg,0.38mmol) as a white amorphous solid in yield: 60 percent.
1H-NMR(500MHz,CDCl3)δ8.08(s,1H),7.74(s,1H),7.50–7.22(m,15H),5.75 (s,1H),4.83(t,J＝8.3Hz,2H),4.69–4.62(m,3H),4.58(d,J＝11.9Hz,1H),4.44–4.38 (m,1H),4.08(d,J＝8.4Hz,1H),3.89(dd,J ═ 10.9,2.6Hz,1H),3.71(dd, J ═ 10.9,3.5Hz, 1H),1.10(s, 3H). Analytical data and literature [ CHO, Aesop; KIM, chong; PARRISH, Jay; XU, jie, Carba-nucleotide analogs for anti viral stream, wo2009132123a1,2009. High resolution mass spectrometry (ESI)+)：C32H34N5O4 +Theoretical value is 552.2605, found 552.2600.
Example 44
Preparation of Compounds 3-4 l:
cooling 5-2a (0.5g, 1.59mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.03mL, 2.07mmol, 2M tetrahydrofuran solution) dropwise, controlling the temperature at-30 deg.C, stirring for reaction at-30 deg.C for 50min, further cooling to-78 deg.C, adding n-BuLi (2.5mL, 3.98mmol, 1.6M hexane solution dropwise), stirring for reaction for 5min, adding 2-1c (2.8g, 4.77mmol) THF (20mL) solution dropwise, maintaining the temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (40mL), brought to room temperature, extracted 4 times with ethyl acetate (20mL × 4), the organic layers combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude product 9-4 h. High resolution mass spectrometry (ESI)+)：C49H48N5O7 +Theoretical value is 818.3548, found 818.3543.
Dissolving the crude product in dry MeCN for 9-4h under nitrogen protection, cooling to-40 deg.C, adding Et3SiH (1.02mL,6.37mmol), stirred for 5min, and then BF was added dropwise3·Et2O (402. mu.L, 3.18mmol), warmed up to room temperature, reacted for 2 hours, after completion of TLC detection, quenched with saturated aqueous sodium bicarbonate (10mL), and the aqueous layer with CH2Cl2Extracting (15 mL. times.3) for 3 times, mixing organic layers, washing with saturated salt solution, drying with anhydrous sodium sulfate, concentrating, and separating and purifying with silica gel column (25:1, V/V)Dichloromethane: methanol) gave 3-4l (726mg,1.03mmol) of a white foamy solid, yield: 65 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 5.8(s,1H, H-1); high resolution mass spectrometry (ESI)+)： C44H40N5O4 +Theoretical value is 702.3075, found 702.3070.
Example 45
Preparation of Compounds 3-4 b:
cooling 5-2b (0.2g, 0.55mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.36mL, 0.72mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (0.87mL, 1.38mmol, 1.6M hexane solution), stirring for 5min, adding 2-1a (719mg, 1.66mmol) THF (10mL), reacting for 2 hr at-78 deg.C, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (20mL), allowed to return to room temperature, extracted 4 times with ethyl acetate (20 mL. times.4), and the organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to give 9-4g of crude product. High resolution mass spectrometry (ESI)+)：C37H42N5O7 +Theoretical value is 668.3079, found 668.3073.
Dissolving 9-4g of crude product obtained above in 10mL of dried MeCN under nitrogen protection, cooling to-40 deg.C, and adding Et3SiH (0.4mL,2.22mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.1mL,1.11mmol), after the dropwise addition, the temperature is naturally raised to room temperature, the reaction is carried out for 2h, after the TLC detection reaction is finished, saturated sodium bicarbonate aqueous solution (10mL) is added for quenching reaction, and the water layer is treated with CH2Cl2Extracting (10 mL. times.3) for 3 times, mixing organic layers, washing with saturated saline, drying over anhydrous sodium sulfate, concentrating, and separating and purifying with silica gel column (25:1, V/V, dichloromethane: methanol) to obtain white pigmentProduct 3-4b (192mg,0.35mmol) as a colored amorphous solid, yield: and 63 percent.
The analytical data are in agreement with products 3-4b of example 43.
Example 46
Preparation of Compounds 3-4 m:
cooling 5-2a (200mg, 0.64mmol) THF (5mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (415 μ L, 0.83mmol, 2M tetrahydrofuran solution) dropwise, controlling temperature at-30 deg.C, stirring for reaction for 50min, further cooling to-78 deg.C, adding n-BuLi (100 μ L, 1.60mmol, 1.6M hexane solution), stirring for reaction for 5min, adding 2-1b (803mg, 1.92mmol) THF (10mL) solution dropwise, maintaining temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, adding saturated NH4The reaction was quenched with aqueous Cl (10mL), returned to room temperature, extracted 4 times with ethyl acetate (10mL × 4), the organic layers were combined, washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated to give crude 9-4 i. High resolution mass spectrometry (ESI)+)：C36H40N5O7 +Theoretical value is 654.2922, found 654.2918.
The crude 9-4i from above was dissolved in 10mL dry MeCN under nitrogen, cooled to-40 deg.C and Et was added3SiH (408. mu.L, 2.56mmol), stirred for 5min, and then BF was added dropwise3·Et2O (162. mu.L, 1.28mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection reaction, quenched with saturated aqueous sodium bicarbonate (10mL), the aqueous layer extracted 3 times with dichloromethane (10 mL. times.3), combined organic layers, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by silica gel column separation (25:1, V/V, dichloromethane: methanol) to give the product as a white amorphous solid 3-4m (203mg,0.38mmol), yield: 59 percent.
1H-NMR(400MHz,MeOD-d4)δ7.97(s,1H),7.46(s,1H),7.33–7.26(m,10H),7.22 –7.18(m,5H) 5.45(d, J ═ 5.2Hz,1H),4.66(d, J ═ 12.1Hz,1H),4.61(d, J ═ 2.9Hz,1H), 4.58(d, J ═ 3.2Hz,1H),4.51(dd, J ═ 12.0,5.1Hz,3H),4.43(t, J ═ 5.1Hz,1H), 4.31-4.26 (m,1H),4.20(t, J ═ 5.2Hz,1H),3.71(dd, J ═ 10.7,3.7Hz,1 ddh), 3.62(dd, J ═ 10.8,4.2 Hz, 1H). Data were analyzed against literature [ LEE, Jae Moon; CHOI, MinJeong, Compounds and compositions for modulating tissue methyl Transferase activity, WO2014035140A2,2014. High resolution mass spectrometry (ESI)+)：C31H32N5O4 +Theoretical value is 538.2449, found 538.2442.
Example 47
Preparation of Compounds 3-4 n:
cooling 5-2a (0.5g, 1.59mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (1.03mL, 2.07mmol, 2M tetrahydrofuran solution) dropwise, controlling the temperature at-30 deg.C, stirring for reaction at-30 deg.C for 50min, further cooling to-78 deg.C, adding n-BuLi (2.5mL, 3.98mmol, 1.6M hexane solution dropwise), stirring for reaction for 5min, adding 2-1d (2.7g, 4.77mmol) THF (20mL) solution dropwise, maintaining the temperature at-78 deg.C, reacting for 2 hr, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (10mL), allowed to return to room temperature, extracted 4 times with ethyl acetate (20 mL. times.4), and the organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to give crude 9-4 j. High resolution mass spectrometry (ESI)+)：C48H46N5O7 +Theoretical value is 804.3392, found 804.3386.
Dissolving the crude product 9-4j in dry MeCN under nitrogen protection, cooling to-40 deg.C, adding Et3SiH (1.02mL,6.37mmol), stirred for 5min, and then BF was added dropwise3·Et2O (402. mu.L, 3.18mmol), naturally warmed to room temperature, reacted for 2h, after TLC detection, quenched with saturated aqueous sodium bicarbonate (10mL), and the aqueous layer with CH2Cl2(15 mL. times.3) extraction was performed 3 times, organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and subjected to silica gel column separation and purification (25:1, V/V, dichloromethane: methanol) to obtain 3 to 4n (712 mg,1.03mmol) as a white amorphous solid product, in terms of yield: 65 percent.
1H-NMR(400MHz,CDCl3) beta-configuration characteristic peak δ 5.6(d, J ═ 5.0Hz,1H, H-1); high resolution mass spectrometry (ESI)+)：C43H38N5O4 +Theoretical value is 688.2918, found 688.2912.
Example 48
Preparation of Compounds 3-4 m:
cooling 5-2b (0.2g, 0.55mmol) THF (10mL) solution to-30 deg.C under anhydrous and oxygen-free conditions, adding diisopropylamino lithium THF solution (0.36mL, 0.72mmol, 2M tetrahydrofuran solution) dropwise, stirring at-30 deg.C, reacting for 50min, cooling to-78 deg.C, adding n-BuLi (0.87mL, 1.38mmol, 1.6M hexane solution), stirring for 5min, adding 2-1b (695mg, 1.66mmol) THF (10mL), reacting for 2 hr at-78 deg.C, detecting by TLC, and adding saturated NH4The reaction was quenched with aqueous Cl (10mL), allowed to return to room temperature, extracted 4 times with ethyl acetate (20 mL. times.4), and the organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to give crude 9-4 i. High resolution mass spectrometry (ESI)+)：C36H40N5O7 +Theoretical value is 654.2922, found 654.2918.
The crude 9-4i from above was dissolved in 10mL dry MeCN under nitrogen, cooled to-40 deg.C and Et was added3SiH (0.4mL,2.22mmol), stirred for 5min, and then BF was added dropwise3·Et2O (0.1mL,1.11mmol), after the dropwise addition, the temperature is naturally raised to room temperature, the reaction is carried out for 2h, after the TLC detection reaction is finished, saturated sodium bicarbonate aqueous solution (10mL) is added for quenching reaction, and the water layer is treated with CH2Cl2(10 mL. times.3) extraction was performed 3 times, organic layers were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and subjected to silica gel column separation and purification (25:1, V/V, dichloromethane: methanol) to obtain a white amorphous solid product 3-4m (214mg,0.40mmol), yield: 72 percent.
The analytical data agree with the products 3 to 4m of example 46.
Claims (4)
1. A method for preparing a C-nucleoside analog, comprising the steps of:
(c) removing N from the compound shown in the general formula 5 in the presence of a metal organic reagent c16-protons, which undergo halogen-metal exchange in the presence of a metal organic reagent C2, undergo an addition reaction with lactone compound 2 to give compound 7, compound 7 is reduced with a reducing agent C4 and lewis acid C4 to give alpha-C-nucleoside analogue 8;
or,
(d) removing N from the compound shown in the general formula 5 in the presence of a metal organic reagent d16-protons, which undergo halogen-metal exchange in the presence of a metal organic reagent d2, undergo an addition reaction with a lactone compound 2-1 to give a compound 9, the compound 9 being reduced to give beta-C-nucleoside analogue 3 by reaction with a reducing agent d4 and a lewis acid d 4;
wherein Hal is selected from chlorine, bromine, iodine;
z is selected from CR0Or nitrogen, R0Is H, halogen, cyano, C1-C6Straight or branched alkyl or C2-C6Straight or branched alkenyl;
R1selected from H or methyl;
R2selected from H or tert-butyloxycarbonyl;
pg is selected from 2-naphthylmethyl, 1-naphthylmethyl, benzyl and p-methoxybenzyl;
the metal-organic reagent c1 and the metal-organic reagent d1 are respectively and independently lithium diisopropylamide;
the metal-organic reagent c2 and the metal-organic reagent d2 are respectively and independently n-butyl lithium;
the reducing agents c4 and d4 are independently triethylsilane;
the Lewis acid catalysts c4 and d4 are independently selected from boron trifluoride diethyl etherate or trimethylsilyl trifluoromethanesulfonate.
2. A process for the preparation of a C-nucleoside analogue according to claim 1, characterized by the steps of:
(a) carrying out amino protection reaction on the amino of the heterocyclic compound 1 to obtain a compound shown as a general formula 5;
(b) hydroxyl of the sugar compound 6 and PgBr or PgCl are subjected to hydroxyl protection reaction to obtain a lactone compound 2; or hydroxyl of the sugar compound 6-1 and PgBr or PgCl are subjected to hydroxyl protection reaction to obtain a lactone compound 2-1;
(c) removing N from the compound shown in the general formula 5 in the presence of a metal organic reagent c16-protons, which undergo halogen-metal exchange in the presence of a metal organic reagent C2, undergo an addition reaction with lactone compound 2 to give compound 7, compound 7 is reduced with a reducing agent C4 and lewis acid C4 to give alpha-C-nucleoside analogue 8;
or,
(d) removing N from compound 5 in the presence of metal organic reagent d16Proton, halogen-metal exchange in the presence of a metal organic reagent d2, addition reaction with a lactone compound 2-1 to obtain a compound 9, reaction of the compound 9 with a reducing agent d4 and a Lewis acid d4, N removal6-tert-butoxycarbonyl protection to give beta-C-nucleoside analogue 3;
wherein,
hal is selected from bromine and iodine;
z is selected from C R0Or nitrogen, R0Is H, cyano or vinyl;
R1selected from H or methyl;
R2selected from H or tert-butyloxycarbonyl;
pg is benzyl, 2-naphthylmethyl or p-methoxybenzyl;
the metal-organic reagent c1 and the metal-organic reagent d1 are respectively and independently lithium diisopropylamide;
the metal-organic reagent c2 and the metal-organic reagent d2 are respectively and independently n-butyl lithium;
the reducing agents c4, d4 are each independently triethylsilane;
the Lewis acid catalysts c4 and d4 are independently selected from boron trifluoride diethyl etherate or trimethylsilyl trifluoromethanesulfonate.
3. The method of preparing a C-nucleoside analog according to claim 1, wherein step (C) is one selected from the group consisting of:
(c-a) removal of N from Compound 5-1a in the Presence of a Metal-organic reagent c16-protons, which undergo halogen-metal exchange in the presence of a metal-organic reagent C2, undergo an addition reaction with a lactone compound 2 to give a compound 7-1, the compound 7-1 is reacted with a reducing agent C4 and a Lewis acid C4 to give an alpha-C-nucleus by reduction8-1 parts of a glycoside analog;
or,
(c-b) removing N from the compound 5-2a in the presence of a metal-organic reagent c16-protons, which undergo halogen-metal exchange in the presence of a metal organic reagent C2, undergo an addition reaction with lactone compound 2 to give compound 7-2, compound 7-2 reacts with a reducing agent C4 and lewis acid C4, and is reduced to give alpha-C-nucleoside analogue 8-2;
wherein,
R1selected from H or methyl;
R2selected from H or tert-butyloxycarbonyl;
pg is 2-naphthylmethyl or benzyl;
the metal organic reagent c1 is lithium diisopropylamide;
the metal organic reagent c2 is n-butyl lithium;
the reducing agent c4 is triethylsilane;
the Lewis acid catalyst c4 is selected from boron trifluoride diethyl etherate or trimethylsilyl trifluoromethanesulfonate.
4. The method of preparing a C-nucleoside analog according to claim 1, wherein step (d) is one selected from the group consisting of:
(d-a) removal of N from Compound 5-1a in the Presence of a Metal-organic reagent d16Proton, halogen-metal exchange in the presence of a metal organic reagent d2, addition reaction with a lactone compound 2-1 to obtain a compound 9-3, reaction of the compound 9-3 with a reducing agent d4 and a Lewis acid d4, N removal6-protection of tert-butyloxycarbonyl to give beta-C-nucleoside analogue 3-3;
or,
(d-b) removal of N from Compound 5-2a in the Presence of a Metal-organic reagent d16Proton, halogen-metal exchange in the presence of a metal organic reagent d2, addition reaction with a lactone compound 2-1 to obtain a compound 9-4, reaction of the compound 9-4 with a reducing agent d4 and a Lewis acid d4, N removal6-tert-butoxycarbonyl protection to give beta-C-nucleoside analogue 3-4;
wherein,
R1selected from H or methyl;
R2selected from H or tert-butyloxycarbonyl;
pg is p-methoxybenzyl or benzyl;
the metal organic reagent d1 is lithium diisopropylamide;
the metal organic reagent d2 is n-butyl lithium;
the reducing agent d4 is triethylsilane;
the Lewis acid catalyst d4 is selected from boron trifluoride diethyl etherate or trimethylsilyl trifluoromethanesulfonate.
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