CN108684665B - Application of compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in inhibiting and removing algae - Google Patents

Application of compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in inhibiting and removing algae Download PDF

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CN108684665B
CN108684665B CN201810456283.9A CN201810456283A CN108684665B CN 108684665 B CN108684665 B CN 108684665B CN 201810456283 A CN201810456283 A CN 201810456283A CN 108684665 B CN108684665 B CN 108684665B
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algae
inhibiting
tetrabromo
biphenol
reagent
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CN108684665A (en
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张永雨
解瑞泽
李德海
孙春晓
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/04—Disinfection

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Abstract

The invention discloses application of a compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in inhibiting algae. The algae killing spectrum experiment proves that the compound has good algae inhibiting effect on 11 kinds of algae such as pseudo-short Alopecuroidis, Chaetoceros muelleri, Prorocentrum micans, Qiangqianchuan algae, Nannochloropsis, Prochlorophosphaeria, Dunaliella salina, Phaeodactylum tricornutum, Chlorella, Platymonas subcordiformis, Porphyroma purpurea and the like, and has important significance for treating natural disasters such as red tide, water bloom and the like and preventing water eutrophication.

Description

Application of compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in inhibiting and removing algae
Technical Field
The invention relates to the field of new application of compounds, in particular to application of a compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in algae inhibition and algae removal.
Background
"Red tide," also known as "Red ghost," is an abnormal phenomenon in marine ecosystems. With the increasing degree of industrialization of human society, a large amount of wastewater containing various nitrogen-containing organic matters is discharged into seawater, thereby promoting the eutrophication of seawater and the mass propagation of red tide algae. Under the action of various influencing factors, red tide algae in the seaweed family are caused by explosive proliferation under specific environmental conditions, so that red tide is caused. As a harmful natural phenomenon, the harm from red tide is also very great:
1. a large amount of red tide organisms gather on gills of the fishes to suffocate the fishes to die due to oxygen deficiency.
2. After the red tide organism dies, the algae consume a large amount of dissolved oxygen in water in the decomposition process, so that the fishes and other marine organisms die due to oxygen deficiency, and simultaneously release a large amount of harmful gas and toxin to seriously pollute the marine environment, so that the normal ecological system of the sea is seriously damaged.
3. Fishes swallow a large amount of toxic algae.
4. Some algae can secrete toxic substances to pollute water and cause death of fishes.
After the red tide occurs, besides the seawater turns red, the pH value of the seawater also rises, the viscosity is increased, and plankton of the non-red tide algae die and decays; red tide algae also die in large numbers due to explosive proliferation and excessive aggregation. In the world, over 260 of the 4000 marine plankton algae can form red tides, and over 70 of them can produce toxins. Some of the secreted toxins can directly cause massive death of marine organisms, and some of the toxins can even be transmitted through a food chain to cause food poisoning of human beings.
In freshwater systems, there is also a similar natural hazard, known as "bloom". The algae in the water body breed in a large quantity, and the water body begins to be blue, green or dark brown. The algae breed in large quantities to become the dominant population in the water body, and the phenomenon of water bloom appears after the waste water containing a large amount of nitrogen and phosphorus in the life and industrial and agricultural production enters the water body. The occurrence of this phenomenon will cause a large number of deaths of fish living in the water body, and the safety of drinking water will be seriously threatened.
The algae-lysing bacteria are a general name of bacteria which inhibit the growth of algae or lyse algae cells in a direct or indirect way, wherein the indirect way of secreting extracellular algae-inhibiting substances is taken as a main term; the micro algae inhibiting substance can inhibit the growth of algae or crack algae cells. The method of treating algae by using bacteria is a red tide treatment mode with important prospect, however, the research on algae inhibiting substances at present has a large blank. Therefore, the development of products with the algae inhibiting effect has important significance for the treatment of red tide and water bloom and the maintenance of the balance of an ecological system.
Disclosure of Invention
Aiming at the prevention and control of natural disasters such as red tide, water bloom and the like, the research finds that the compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol is a new application of the algae inhibiting substance, and has good inhibiting effect on various algae in the water body in the nature.
In order to achieve the technical purpose, the invention provides the following technical scheme:
the invention aims to provide application of a compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in the aspect of algae inhibition.
The structure of the compound is shown as the following figure:
Figure BDA0001659756330000021
the algae killing spectrum experiment proves that the compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol has an inhibiting effect on the growth of various algae, has a strong algae inhibiting effect on partial algae, and has an algae inhibiting rate of over 75 percent.
The invention also aims to provide an algae inhibiting reagent, which mainly comprises a preparation solution of a compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol, and also comprises other bacterial extracts with algae inhibiting effect.
Preferably, the concentration of the 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol preparation solution is 10. mu.g/mL.
The invention also aims to provide an algae inhibiting kit, which is characterized by comprising the algae inhibiting reagent.
The fourth purpose of the invention is to provide an algae inhibiting method, namely, the reagent is added into algae liquid.
Preferably, the algae inhibiting reagent is added into the algae liquid according to the proportion of 5%.
Preferably, the culture conditions of the algae liquid are 20 ℃, the illumination intensity is 3000lx, and the light-dark period is 12h:12 h.
Preferably, the algae targeted by the algae inhibiting method are Thalassiosira pseudonana, Chaetoceros muelleri, Prorocentrum micans, Prinsepia robusta, Nannochloropsis, Prochlorophyces, Dunaliella salina, Phaeodactylum tricornutum, Chlorella, Platymonas subcordiformis, and Porphyromonas.
Further, it is selected from the group consisting of Alternaria pseudonana, Chaetoceros muelleri, Prorocentrum micans, Prinsepia robusta, Nannochloropsis, Prochlorophosphaeria, and Dunaliella salina.
The fifth purpose of the invention is to provide the application of the algae inhibiting reagent, the algae inhibiting kit and the method in the aspects of treating red tide and water bloom, preventing eutrophication of water bodies in fish ponds and the like.
The invention has the advantages of
The compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol is mostly used as an intermediate for chemical synthesis in the prior art, and the application of the compound is less researched in the current research. The research of the invention finds a brand-new application of the compound, and makes contribution to the further development of the compound.
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The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application.
FIG. 1 is a graph showing the algistatic effect of 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol on Synechococcus PCC7002 at different doses;
wherein, the compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol solution is added into the algae solution for five days.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
One embodiment of the invention is to provide application of a compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in inhibiting algae. The algae killing spectrum experiment proves that the compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol has an inhibiting effect on the growth of various algae, has a strong algae inhibiting effect on part of algae, and has an algae inhibiting rate of over 75 percent.
In another embodiment of the invention, an algistat is provided, which is a solution of 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol and other algae-inhibiting bacterial extracts.
In a preferred embodiment, the concentration of the prepared solution is 10. mu.g/mL.
In another embodiment of the present invention, there is provided an algal-inhibiting kit comprising the above-mentioned algal-inhibiting reagent.
In another embodiment of the present invention, there is provided an algal inhibition method comprising adding the above-mentioned reagent to an algal solution.
In a preferred embodiment, the above-mentioned algistat is added to the algae solution in a proportion of 5%.
In a preferred embodiment, the culture conditions of the algal solution are 20 ℃, 3000lx of light intensity, and 12 h/12 h of light dark cycle.
In a preferred embodiment, the algae targeted by the algae-inhibiting method is selected from the group consisting of Alstonia pseudonana, Chaetoceros muelleri, Prorocentrum micans, Qiangchong algae, Nannochloropsis, Prochloraceae, Dunaliella salina, Phaeodactylum tricornutum, Chlorella, Platymonas subcordiformis, and Porphyromonas.
In a further preferred embodiment, the plant is selected from the group consisting of Alternaria pseudonana, Chaetoceros muelleri, Prorocentrum micans, Prinsepia robusta, Nannochloropsis, Prochlorophyces, Dunaliella salina.
In another embodiment of the invention, the algae inhibiting reagent, the algae inhibiting kit and the method are applied to the treatment of red tide and water bloom and the prevention of eutrophication of a water body in a fish pond.
Example 1 calculation method of algal inhibition Activity
1. Test algal species and culture conditions
Selecting synechococcus PCC7002 as a tested alga species, culturing under the conditions of 26 ℃ of temperature, 3000lx of illumination intensity and 12 h/12 h of light dark cycle, culturing to an exponential phase, and then subpackaging into 50mL triangular flasks, wherein each flask is subpackaged with 20mL alga liquid.
2. Calculation of algal inhibition rate
1mL of the component to be tested, which is 10. mu.g/mL, is added into the synechococcus PCC7002 algae solution, cultured for 5 days, and counted under a fluorescence microscope.
The algae inhibiting rate is calculated according to the following formula
Algal inhibition rate (Algicidal rate) ═ NC-NT)/NC×100％
NCNumber of viable cells in control group, NTThe number of viable cells in the experimental group is shown.
The algae inhibiting rate of more than 75 percent is regarded as strong algae inhibiting effect, 50 to 75 percent is regarded as the algae inhibiting effect, and less than 50 percent is regarded as no algae inhibiting effect.
EXAMPLE 2 algal inhibition Range of the Compound
The algae killing spectrum experiment is carried out on 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol by selecting pseudo-short sea chain algae, Chaetoceros muelleri, Prorocentrum micans, Qiangqianchui algae, Micrococcus nanensis, Prochloraceae, Dunaliella salina, Phaeodactylum tricornutum, Chlorella, Platymonas subcordiformis and Porphyromonas sp. The culture conditions were: the temperature is 20 ℃, the illumination intensity is 3000lx, and the light dark period is 12h:12 h. 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol of 10. mu.g/mL was added to the above 11 kinds of algal solutions in a proportion of 5%, and the algal inhibition rate was calculated according to the calculation formula of algal inhibition rate in example 1.
The experimental result shows that the 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol has strong algae inhibiting effect (the algae inhibiting rate is more than 75%) on the pseudo-short sea chain algae, Chaetoceros muelleri, Prorocentrum micans, Qianchonaea robusta, nannochloropsis miniata, protochlorella and Dunaliella salina, and has the algae inhibiting effect (the algae inhibiting rate is 50-75%) on the phaeodactylum tricornutum, chlorella vulgaris, Platymonas subcordiformis and Porphyromonas. The results are shown in the following table:
Figure BDA0001659756330000051
the above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (12)

1. The compound 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol is applied to the aspect of algae inhibition.
2. The use of claim 1, wherein the algae is selected from the group consisting of Alstonia pseudonana, Chaetoceros muelleri, Prorocentrum micans, Primordia robusta, Nannochloropsis, Prochloraceae, Dunaliella salina, Phaeodactylum tricornutum, Chlorella vulgaris, Platymonas subcordiformis, and Porphyromonas.
3. The use of claim 2, wherein the algae is selected from the group consisting of Alstonia pseudonana, Chaetoceros muelleri, Prorocentrum micans, Primordia robusta, Nannochloropsis, Prochloraceae, Dunaliella salina.
4. The algae inhibiting reagent is a preparation solution of 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol, and also comprises other algae inhibiting bacteria extracts.
5. The algae inhibiting reagent is characterized in that the concentration of 3,3 ', 5,5 ' -tetrabromo-2, 2 ' -biphenol in the reagent is 10 mu g/mL.
6. An algal inhibition kit comprising the reagent according to claim 4.
7. An algal inhibition method characterized by adding the reagent according to claim 4 to an algal solution.
8. The method of claim 7, wherein the reagent is added to the algal solution at a rate of 5%.
9. The method of claim 7, wherein the algal broth culture conditions are: the temperature is 20 ℃, the illumination intensity is 3000lx, and the light dark period is 12h:12 h.
10. The method of claim 7, wherein the algae is selected from the group consisting of Alstonia pseudonana, Chaetoceros muelleri, Prorocentrum micans, Primordia robusta, Nannochloropsis, Prochloraceae, Dunaliella salina, Phaeodactylum tricornutum, Chlorella vulgaris, Platymonas subcordiformis, and Porphyromonas.
11. The method of claim 10, wherein the algae is selected from the group consisting of Alstonia pseudonana, Chaetoceros muelleri, Prorocentrum micans, Primordia robusta, Nannochloropsis, Prochloranthus, Dunaliella salina.
12. The application of the algae inhibiting reagent of claim 4 and the algae inhibiting kit of claim 6 in treating red tide and water bloom and preventing eutrophication of water body in fish pond.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2346001A1 (en) * 1975-03-11 1977-10-28 Dick Pierre Pharmaceutical compsn. contg. tetra:halo di:phenol - for treatment of fasciola hepatica and fasciola gigantica in mammals
US20090163692A1 (en) * 2007-12-21 2009-06-25 General Electric Company Aromatic polyethers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2346001A1 (en) * 1975-03-11 1977-10-28 Dick Pierre Pharmaceutical compsn. contg. tetra:halo di:phenol - for treatment of fasciola hepatica and fasciola gigantica in mammals
US20090163692A1 (en) * 2007-12-21 2009-06-25 General Electric Company Aromatic polyethers

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