CN108265003A - Multichannel gene sequencing reaction cell and multichannel gene sequencing reaction device - Google Patents
Multichannel gene sequencing reaction cell and multichannel gene sequencing reaction device Download PDFInfo
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- CN108265003A CN108265003A CN201611263452.4A CN201611263452A CN108265003A CN 108265003 A CN108265003 A CN 108265003A CN 201611263452 A CN201611263452 A CN 201611263452A CN 108265003 A CN108265003 A CN 108265003A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to biochemical equipment technical fields, provide a kind of multichannel gene sequencing reaction cell；Including top substrate layer, underlying substrate and a pair of of adapter；Multiple reactive tanks are provided on underlying substrate, top substrate layer is fitted on underlying substrate and closes reactive tank, and top substrate layer is provided with multiple reagent inlets and reagent exit；First through hole is provided on adapter, adapter is internally provided with a plurality of liquid separation channel and multiple liquid separation holes, first through hole are connected with liquid separation channel, and the distance between first through hole and each liquid separation hole are equal；One of adapter is tightly attached in top substrate layer, and each liquid separation hole is connected respectively with a reagent inlet of top substrate layer；Another adapter is tightly attached in top substrate layer, and each liquid separation hole is connected respectively with a reagent exit of top substrate layer；The beneficial effects of the invention are as follows：The flow velocity of reagent is identical in reaction channel, simplifies the structure of multichannel gene sequencing reaction cell.
Description
Technical field
The present invention relates to biochemical equipment technical field, more particularly, it relates to which multichannel gene sequencing reaction is small
Room and multichannel gene sequencing reaction device.
Background technology
Gene sequencing reaction device includes the reaction small chamber for carrying out gene sequencing reaction, and reaction small chamber has at least
One reaction channel, by being passed through reagent into reaction channel with producer sequencing reaction.
A kind of multichannel gene sequencing reaction cell of the prior art, including coverslip, interlayer sheet and glass slide, and
Coverslip, interlayer sheet and glass slide are bonded successively, multiple grooves are provided in the interlayer sheet, while on the cover slip
Multiple through-holes are provided with, after coverslip, interlayer sheet and glass slide fit, through-hole is connected with the groove, and groove is first
The through-hole of tail end is respectively then reagent inlet and reagent exit, and reaction reagent is passed through into groove by reagent inlet, to occur
Gene sequencing reaction.Since multichannel gene sequencing reaction cell has multiple reaction channels, need to be carried out at the same time gene sequencing
Reaction, it is therefore desirable to reagent is passed through into reaction channel simultaneously from multiple reagent inlets, in the prior art, on each reagent inlet
Reagent pipeline is respectively connected with, by controlling the switch of reagent pipeline, while reagent is passed through into reaction channel.But this structure
Multichannel gene sequencing reaction cell, due to the presence of a plurality of reagent pipeline, structure is complex, and is susceptible to leakage
Problem influences being normally carried out for gene sequencing reaction.
Therefore a kind of new multichannel gene sequencing reaction cell and multichannel gene sequencing reaction device, Neng Goutong are needed
When inject reagent into multiple reaction channels, and the problem of simplify the structure of sequencing reaction small chamber, avoid the occurrence of leakage.
Invention content
The purpose of the present invention is to provide a kind of multichannel gene sequencing reaction cells, it is intended to solve mostly logical in the prior art
The problem of dao gene sequencing reaction small chamber is complicated.
In order to realize goal of the invention, a kind of multichannel gene sequencing reaction cell is provided, is theed improvement is that：Including upper
Laminar substrate, underlying substrate and a pair of of adapter；Multiple reactive tanks, top substrate layer patch are provided on the underlying substrate
It closes on underlying substrate and closes the reactive tank, the top substrate layer is provided with multiple reagents being connected with reactive tank and enters
Mouth and reagent exit；
Be provided with the first through hole for being passed through reagent on the adapter, adapter be internally provided with a plurality of liquid separation channel and
The multiple liquid separation holes being connected with liquid separation channel, the first through hole are connected with liquid separation channel, and the first through hole with it is every
The distance between a liquid separation hole is equal；
One of adapter is close to the side of top substrate layer, and each liquid separation hole respectively with a reagent inlet of top substrate layer
It is connected；Another adapter is close to the opposite side of top substrate layer, and each liquid separation hole respectively with a reagent of top substrate layer
Outlet is connected.
In above-mentioned structure, the reactive tank includes the inlet region, reaction zone and the outlet area that are sequentially communicated, the entrance
The width of area and outlet area is respectively less than the width of the reaction zone, and the reagent inlet access inlet region is described
The reagent exit access outlet area.
Further, there is the underlying substrate upper surface and opposite two sides, reactive tank to be arranged on underlying substrate
Upper surface, the two sides of the underlying substrate are both provided with multiple limiting grooves, and between the adjacent limiting groove of each side
Protrusion is formed, the inlet region and outlet area are separately positioned on the protrusion of two sides of underlying substrate.
In above-mentioned structure, the adapter includes briquetting and connector；
The connector have first surface and second surface, the liquid separation channel be arranged on it is recessed on connector first surface
Slot, the liquid separation hole is through first surface and second surface；
The briquetting is pressed on the first surface of connector, and the second surface of the connector is tightly attached to the top substrate layer
On.
Further, a fixed groove is provided on the briquetting, the first through hole is arranged in fixed groove, and
One connecting tube is installed, the connecting tube is connected with first through hole in fixed groove.
Further, a notch is provided on the liquid separation channel, which is reagent inlet, and the briquetting is pressed on
After on the first surface of connector, the first through hole of the briquetting is connected with the reagent inlet of the connector, described
Reagent inlet and the distance in each liquid separation hole are equal.
Further, the first surface of the connector is provided with the first liquid separation channel, the second liquid separation channel, third liquid separation
Channel, the 4th liquid separation channel and the 5th liquid separation channel；
The reagent inlet is set to the center of the first liquid separation channel, and one end of two the second liquid separation channels is respectively communicated with
On the both ends of the first liquid separation channel；
The other end of two the second liquid separation channels is respectively communicated in the center of two third liquid separation channels, four the 4th liquid separations
One end of channel is respectively communicated on the both ends of two third liquid separation channels；
The other end of four the 4th liquid separation channels is respectively communicated in the center of four the 5th liquid separation channels；On the connector
Eight liquid separation holes are provided with, and eight liquid separation holes are located at the both ends of four the 5th liquid separation channels respectively.
Further, the second liquid separation channel is perpendicular to the first liquid separation channel, and the third liquid separation channel is perpendicular to
Two liquid separation channels, the 4th liquid separation channel is perpendicular to third liquid separation channel, and the 5th liquid separation channel is perpendicular to the 4th liquid separation
Channel；
Two second liquid separation channels are located at the same side of the first liquid separation channel；Four the 4th equal positions of liquid separation channel
In the same side of third liquid separation channel, and toward the first liquid separation channel.
Further, the second surface of the connector is provided with multiple liquid separation cavitys, and the liquid separation hole is connected to described
In liquid separation cavity, and each liquid separation cavity is connected respectively with a reagent inlet of sequencing reaction small chamber.
In above-mentioned structure, the upper surface of the top substrate layer is coated with heating coating.
The present invention also provides a kind of multichannel gene sequencing reaction devices, the improvement is that：Including multichannel base
Because of sequencing reaction small chamber and cell installing plate, multichannel gene sequencing reaction cell is mounted on the cell installing plate；
There is the underlying substrate of the multichannel gene sequencing reaction cell upper surface and opposite two sides, reactive tank to be arranged on
The upper surface of underlying substrate, the two sides of the underlying substrate are both provided with multiple limiting grooves, and the adjacent limit of each side
Protrusion is formed between the groove of position；
The first installation through-hole is provided on the cell installing plate, with opposite interior in the first installation through-hole of cell installing plate
Side, and the multiple limit protrusions stretched into the first installation through-hole are provided on medial surface, the adjacent limit convex of each medial surface
Limiting gap is formed between rising；
The underlying substrate of the multichannel gene sequencing reaction cell is located in the first installation through-hole of cell installing plate, and lower floor
The protrusion of substrate is inserted into the limiting gap of cell installing plate, and the limit protrusion of the cell installing plate is inserted into underlying substrate
In limiting groove.
Further, the cell installing plate is provided at both ends with limited block, the multichannel gene sequencing reaction cell
Top substrate layer be located between two limited blocks；
Locating piece, the side of the top substrate layer of the multichannel gene sequencing reaction cell are additionally provided on the cell installing plate
It is resisted against on the locating piece.
Further, the multichannel gene sequencing reaction device further includes fixed seat and rotating turret；
The second installation through-hole is provided in the fixed seat, the lower surface of fixed seat is fixed at the both ends of the cell installing plate,
Multichannel gene sequencing reaction cell is made to be located at the lower position of the second installation through-hole；
The rotating turret is rotatably installed in the second installation through-hole of fixed seat, and the rotating turret is anti-towards multichannel gene sequencing
The direction of cell is answered to be pressed on the adapter after rotating.
Further, conductive thimble is installed, the rotating turret is towards multichannel gene sequencing reaction on the rotating turret
After the direction rotation of cell, the conduction thimble is in contact with heating coating.
Further, it is rotatablely equipped with a wedge-shaped limited block on the fixed frame, the rotating turret is towards multichannel gene
After the direction rotation of sequencing reaction small chamber, the wedge shape limited block is then pressed on the rotating turret.
Further, a coil holder is installed on the rotating turret, multiple mounting holes is provided on the coil holder.
From the foregoing, it will be observed that from the reagent that reagent inlet flows into, each liquid separation hole is uniformly flowed to so that each liquid separation
The flow velocity of reagent is equal in channel, while reaches each liquid separation hole, while flows into the more of multichannel gene sequencing reaction cell
In a reaction channel so that the flow velocity of reagent is identical in reaction channel, to be carried out at the same time the gene sequencing reaction of multichannel；It is mostly logical
Each reaction channel of dao gene sequencing reaction small chamber is passed through the pipeline of reagent without individually connection, simplifies multichannel base
Because of the structure of sequencing reaction small chamber, simultaneously because eliminating multiple pipelines, avoid pipeline junction and the situation of leakage occur, and
And its installation is very convenient, fast with dismantling.
Description of the drawings
Fig. 1 is the configuration schematic diagram of multichannel gene sequencing reaction cell in one embodiment of the invention.
Fig. 2 is the stereochemical structure signal of the adapter of multichannel gene sequencing reaction cell in one embodiment of the invention
Figure.
Fig. 3 is that the first detonation configuration of the adapter of multichannel gene sequencing reaction cell in one embodiment of the invention shows
It is intended to.
Fig. 4 is that the second detonation configuration of the adapter of multichannel gene sequencing reaction cell in one embodiment of the invention shows
It is intended to.
Fig. 5 is the three-dimensional knot of the connector of the adapter of multichannel gene sequencing reaction cell in one embodiment of the invention
Structure schematic diagram.
Fig. 6 is the vertical view of the adapter of multichannel gene sequencing reaction cell in one embodiment of the invention.
Fig. 7 is diagrammatic cross-section at A-A in Fig. 6.
Fig. 8 is that the liquid separation of the connector of the adapter of multichannel gene sequencing reaction cell in one embodiment of the invention is led to
The positive structure schematic in road.
Fig. 9 is the liquid separation of the connector of the adapter of multichannel gene sequencing reaction cell in another embodiment of the present invention
The positive structure schematic of channel.
Figure 10 is point of the connector of the adapter of multichannel gene sequencing reaction cell in another embodiment of the present invention
The positive structure schematic of liquid channel.
Figure 11 is the vertical of the connector of the adapter of multichannel gene sequencing reaction cell in another embodiment of the present invention
Body structure diagram.
Figure 12 is the Application Example of the adapter of multichannel gene sequencing reaction cell in another embodiment of the present invention
Figure.
Figure 13 is the structure diagram of multichannel gene sequencing reaction device in one embodiment of the invention.
Figure 14 is the configuration schematic diagram of multichannel gene sequencing reaction device in one embodiment of the invention.
Figure 15 is the dimensional structure diagram of multichannel gene sequencing reaction device in another embodiment of the present invention.
Figure 16 is the configuration schematic diagram of multichannel gene sequencing reaction device in another embodiment of the present invention.
Figure 17 is the sequencing reaction small chamber and conduction of multichannel gene sequencing reaction device in another embodiment of the present invention
The dimensional structure diagram of thimble.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, it is right
The present invention is further elaborated.
The present invention proposes first embodiment, and the present embodiment proposes a kind of multichannel gene sequencing reaction cell, by this
Realize that multiple channels are carried out at the same time gene sequencing reaction in multichannel gene sequencing reaction cell.It is specifically, as shown in Figure 1, mostly logical
Dao gene sequencing reaction small chamber includes top substrate layer 50, underlying substrate 60 and a pair of of adapter 70；On the underlying substrate 60
Multiple reactive tanks 601 are provided with, the top substrate layer 50 is fitted on underlying substrate 60 and closes the reactive tank 601,
Gene sequencing reaction is carried out so as to form reaction channel, top substrate layer 50 is provided with multiple reagents being connected with reactive tank 601
Entrance 401 and reagent exit 402, wherein each reactive tank 601 are corresponding including there are one reagent inlet 401 and a reagents
Outlet 402, by being passed through reaction reagent to reagent inlet 401, reagent is flowed into reaction channel, and gene is carried out in reaction channel
Sequencing reaction, and reagent is discharged from reagent exit 402；Multiple reactive tanks 601 are provided on underlying substrate 60, therefore can be realized
Multichannel sequencing reaction.Further, first through hole is provided on the adapter 70, adapter 70 is internally provided with a plurality of
Liquid separation channel and the multiple liquid separation holes being connected with liquid separation channel, the first through hole are connected, and described with liquid separation channel
The distance between first through hole and each liquid separation hole are equal；In the present embodiment, one of adapter 70 is tightly attached to upper strata
On substrate 50, and each liquid separation hole is connected respectively with a reagent inlet 401 of top substrate layer 50；Another adapter 70 is tight
It is attached in top substrate layer 50, and each liquid separation hole is connected respectively with a reagent exit 402 of top substrate layer 50.
In the present embodiment, when needing to carry out gene sequencing reaction, the first through hole of adapter 70 is used to be passed through reagent,
By the effect of liquid separation channel in adapter 70, reagent is uniformly divided into multiple equal portions and is delivered in multiple liquid separation holes,
Even flows to each liquid separation hole so that and the flow velocity of reagent is equal in each liquid separation channel, while reaches each liquid separation hole,
It flowing into multiple reaction channels of multichannel gene sequencing reaction cell simultaneously so that the flow velocity of reagent is identical in reaction channel,
To be carried out at the same time the gene sequencing reaction of multichannel.
In addition, in the above-described embodiment, for underlying substrate 60, the present invention proposes a specific embodiment, described
Underlying substrate 60 has upper surface 602 and opposite two sides 603, and it is recessed that the two sides of underlying substrate 60 are both provided with multiple limitings
Slot 6031, and form protrusion 6032 between the adjacent limiting groove 6031 of each side 603；The reactive tank 601 is set
In the upper surface of underlying substrate 60, reactive tank 601 includes the inlet region 6011, reaction zone 6012 and the outlet area that are sequentially communicated
6013, the inlet region 6011 and outlet area 6013 are arranged on the protrusion of underlying substrate 60,6011 He of inlet region
The width of outlet area 6013 is respectively less than the width of the reaction zone 6012；After top substrate layer 50 and underlying substrate 60 fit,
The reagent inlet 401 of top substrate layer 50 then accesses the inlet region 6011, and the reagent exit 402 of top substrate layer 50 then accesses institute
The outlet area 6013 stated, by the design of this structure, the reagent being passed through is then by reagent inlet 401, into inlet region 6011,
Gene sequencing reaction, since the width of reaction zone 6012 is wider, area bigger, in reaction zone 6012 are carried out in reaction zone 6012
When carrying out gene sequencing reaction, more conducively the fluorescence sent out is captured when sequencing reaction carries out, is surveyed so as to improve gene
The precision of sequence reaction.
For the adapter, the present invention proposes a specific embodiment, as shown in Fig. 2, Fig. 3 and Fig. 4, multichannel
The adapter of gene sequencing reaction cell includes briquetting 10 and connector 20, and the connector 20 has opposite first surface
201 and second surface 202, liquid separation channel 203 is provided on the first surface 201 of connector 20 and multiple through the first table
Face 201 and the liquid separation hole 204 of second surface 202, wherein, in the present embodiment, the liquid separation channel 203 is to be arranged on the
Groove on one surface 201, multiple liquid separation holes 204 are connected by the liquid separation channel 203；10 middle part of briquetting has
Upward protrusion is provided with a fixed groove 101 in the protrusion, a connecting tube 30 is equipped in fixed groove 101, described consolidates
Determine to be provided through the first through hole 102 in briquetting 10 in groove 101, the connecting tube 30 is connected with first through hole 102.When
After the briquetting 10 is pressed on first surface 201, the first through hole 102 of briquetting 10 then with the liquid separation channel of connector 20
203 are connected, and distance is equal between first through hole 102 and each liquid separation hole 204.In the present embodiment, the liquid separation
A notch is provided on channel 203, which is reagent inlet 205, and the briquetting 10 is pressed on the first table of connector 20
After on face 201, the first through hole 102 of the briquetting 10 is connected, and inject with the reagent inlet 205 of the connector 20
Mouth and the distance in each liquid separation hole 204 are equal.
When needing to carry out multichannel gene sequencing reaction, the second surface 202 of the connector 20 is tightly attached to sequencing
Be provided with multiple reagent inlets on reaction small chamber, on sequencing reaction small chamber, each liquid separation hole 204 of connector 20 respectively with sequencing
One reagent inlet of reaction small chamber is connected；As shown in Figures 2 to 7, connecting tube 30 is connected with the external pipeline for being passed through reagent
It is logical, the internal thread to match with the external screw thread of connecting tube 30 is provided in fixed groove 101, there is pipe in the connecting tube 30
Mouthful, the bottom of connecting tube 30 is provided with the cavity 301 of taper, and the cavity 301 of the taper is provided with a gasket 3011, when stubborn
After being close to take over 30, the gasket 3011 in the cavity 301 of taper is then pressed on the bottom surface of fixed groove 101, and seal
The second through-hole is provided on pad 3011, the nozzle of connecting tube 30 is connected with first through hole 102 by the second through-hole；Pass through this
Kind structure prevents leakage occur between connecting tube 30 and briquetting 10；Reagent flows into liquid separation channel from connecting tube 30, first through hole 102
203 reagent inlet 205, since briquetting 10 is bonded on first surface 201, briquetting 10 closes liquid separation channel 203, flows into
The reagent of reagent inlet 205 then by the effect of liquid separation channel 203, flows to multiple liquid separation holes 204, due in the present embodiment
In, the distance between reagent inlet 205 and each liquid separation hole 204 are equal, when the multichannel gene sequencing reaction of the present invention
When the adapter of cell is horizontal positioned, from the reagent of the inflow of reagent inlet 205, each liquid separation hole 204 is uniformly flowed to, is made
The flow velocity for obtaining reagent in each liquid separation channel 203 is equal, while reaches each liquid separation hole 204, while flow into multichannel base
Because in multiple reaction channels of sequencing reaction small chamber so that the flow velocity of reagent is identical in reaction channel, to be carried out at the same time multichannel
Gene sequencing reaction；The present invention by above-mentioned structure, each reaction channel of multichannel gene sequencing reaction cell without
The pipeline for being passed through reagent need to be individually connected, simplifies the structure of multichannel gene sequencing reaction cell, simultaneously because eliminating
Multiple pipelines avoid pipeline junction and the situation of leakage occur, and its installation is very convenient, fast with dismantling.
For the liquid separation channel 203, the invention also provides an embodiment, in the present embodiment, the liquid separation is led to
Road 203 is the groove for being arranged on 10 lower surface of briquetting, after briquetting 10 is pressed on the first surface 201 of connector 20, first
The groove on 10 lower surface of briquetting is closed on surface 201, to form the liquid separation channel 203.Furthermore it is also possible in briquetting 10
Lower surface and connector 20 first surface 201 on simultaneously groove is set, likewise, when briquetting 10 is pressed on connector 20
After on first surface 201, the groove of 10 lower surface of briquetting is then mutually enclosed with the groove on first surface 201, is collectively formed described
Reaction channel.
For the adapter of the multichannel gene sequencing reaction cell, on the basis of above-described embodiment, the present invention
Also proposed an embodiment, as shown in Figures 5 to 7, be provided on the first surface 201 of the connector 20 eight through
The liquid separation hole 204 of first surface 201 and second surface 202, eight liquid separation holes 204 are located along the same line, in the present embodiment, institute
It states second surface 202 and is provided with multiple liquid separation cavitys 2020, the liquid separation hole 204 is connected in the liquid separation cavity 2020, and
And sealing ring 2021 is mounted in each liquid separation cavity 2020, the middle part of sealing ring 2021 is provided with to be connected with liquid separation hole 204
Logical third through-hole 2022, when the second surface 202 of connector 20 is tightly attached on sequencing reaction small chamber, in liquid separation cavity 2020
Sealing ring 2021 be then pressed on sequencing reaction small chamber, and third through-hole 2022 on sealing ring 2021 and sequencing reaction are small
One reagent inlet of room is connected, and so as to which liquid separation hole 204 be made to be connected with the reagent inlet of sequencing reaction small chamber, prevents simultaneously
There is leakage between connector 20 and sequencing reaction small chamber, play good sealing function.
Further, on the basis of Fig. 5 to embodiment shown in Fig. 7, as shown in figure 8, the first table of the connector 20
Face 201 is provided with the first liquid separation channel 2031, the second liquid separation channel 2032, third liquid separation channel 2033, the 4th liquid separation channel
2034 and the 5th liquid separation channel 2035, in the present embodiment, for the position relationship of the liquid separation channel 203, the present invention carries
A specific embodiment is supplied, the reagent inlet 205 is set to the center of the first liquid separation channel 2031, reagent inlet
205 be a semicircular notch, and one end of two the second liquid separation channels 2032 is respectively communicated in the first liquid separation channel
On 2031 both ends；The other end of two the second liquid separation channels 2032 is respectively communicated at the center of two third liquid separation channels 2033
Position, one end of four the 4th liquid separation channels 2034 are respectively communicated on the both ends of two third liquid separation channels 2033；Four
The other end of four liquid separation channels 2034 is respectively communicated in the center of four the 5th liquid separation channels 2035；On the connector 20
Eight liquid separation holes 204 are provided with, and eight liquid separation holes 204 are located at the both ends of four the 5th liquid separation channels 2035 respectively；This knot
The design of structure so that reagent inlet 205 is equal with the distance of any one in eight liquid separation holes 204 so that each
The flow velocity of reagent is equal in liquid separation channel 203, while reaches each liquid separation hole 204, while it is anti-to flow into multichannel gene sequencing
It answers in multiple reaction channels of cell, carries out the gene sequencing reaction of multichannel；In addition, eight liquid separation holes 204 are located at always
On line, in order to realize the design of gene sequencing reaction cell structure.
For the liquid separation channel 203, the invention also provides an embodiment, as shown in figure 9, likewise, the company
The first surface 201 of connector 20 be provided with the first liquid separation channel 2031, the second liquid separation channel 2032, third liquid separation channel 2033,
4th liquid separation channel 2034 and the 5th liquid separation channel 2035, wherein the first liquid separation channel 2031, the second liquid separation channel 2032,
Implementation described in the connection relation and Fig. 6 of three liquid separation channels 2033, the 4th liquid separation channel 2034 and the 5th liquid separation channel 2035
Identical in example, still, in the present embodiment, the second liquid separation channel 2032 is perpendicular to the first liquid separation channel 2031, and described the
Three liquid separation channels 2033 are perpendicular to the second liquid separation channel 2032, and the 4th liquid separation channel 2034 is perpendicular to third liquid separation channel
2033, the 5th liquid separation channel 2035 is perpendicular to the 4th liquid separation channel 2034, also, described two the second liquid separation channels
2032 are located at the same side of the first liquid separation channel 2031；Four the 4th liquid separation channels 2034 are respectively positioned on third liquid separation channel
2033 the same side, and toward the first liquid separation channel 2031, pass through the design of this structure so that eight liquid separation holes
204 are respectively positioned on same straight line, meanwhile, reduce the width of connector 20 so that the whole volume smaller of connector 20, connection
Space occupied by first 20 then smaller, the volume smaller of multichannel gene sequencing reaction cell entirety.
It should be further noted that the adapter of the multichannel gene sequencing reaction cell of the present invention, is not limited in
It applies on eight channel sequencing reaction small chambers, as shown in Figure 10, Figure 11, the first surface 201 of connector 20 is provided with first point
Liquid channel 2031, the second liquid separation channel 2032 and third liquid separation channel 2033, the reagent inlet 205 are set to first point
The center of liquid channel 2031, one end of two the second liquid separation channels 2032 are respectively communicated in the first liquid separation channel 2031
Both ends on, the other end of two the second liquid separation channels 2032 is respectively communicated with the centre bit in two third liquid separation channels 2033
It puts, four liquid separation holes 204 are then located on the both ends of two third liquid separation channels 2033 respectively；Likewise, the of connector 20
There are four liquid separation cavity 2020, the liquid separation hole 204 is connected in the liquid separation cavity 2020 setting of two surfaces 202, and every
Sealing ring is mounted in a liquid separation cavity 2020, the through-hole being connected with liquid separation hole 204 is provided in the middle part of sealing ring, when even
When the second surface 202 of connector 20 is tightly attached on sequencing reaction small chamber, the sealing ring in liquid separation cavity 2020 is then pressed on sequencing
On reaction small chamber, and the through-hole on sealing ring is connected with sequencing reaction small chamber reagent inlet, so as to make liquid separation hole
204 are connected with the reagent inlet of sequencing reaction small chamber, while prevent leakage occur between connector 20 and sequencing reaction small chamber,
Play good sealing function.
As shown in figure 12, for the adapter of the multichannel gene sequencing reaction cell, the present invention is in above-mentioned implementation
On the basis of example, an embodiment is further proposed, multichannel gene sequencing reaction cell 40 is with logical with eight reactions
Road, wherein each reaction channel is respectively provided with reagent inlet 401 and reagent exit 402, therefore multichannel gene sequencing reaction cell
Adapter then set there are two, eight liquid separation holes on the connector 20 of one of adapter are surveyed respectively with multichannel gene
Eight reagent inlets 401 of sequence reaction small chamber 40 are connected, and eight liquid separation holes on the connector 20 of another adapter are then divided
It is not connected with eight reagent exits 402 of multichannel gene sequencing reaction cell 40, realizes that multichannel gene sequencing reaction is small
Eight channels of room 40 are sequenced simultaneously, real by the collective effect of two adapters when carrying out multichannel gene sequencing reaction
Existing eight channels inject simultaneously and discharge reaction reagent.
On the basis of above-described embodiment, the invention also provides a kind of multichannel gene sequencing reaction device, as Figure 13,
Shown in Figure 14, which includes multichannel gene sequencing reaction cell 40, which surveys
Sequence reaction small chamber 40 includes top substrate layer 50, underlying substrate 60 and adapter 70, and adapter 70 includes briquetting 10 and connector
20, for structure and the phase in above-described embodiment of the top substrate layer 50, underlying substrate 60, briquetting 10 and connector 20
Together, therefore in the present embodiment it is then no longer described in detail.In addition, as shown in Figure 13, Figure 14, the multichannel gene sequencing reaction
Device further includes cell installing plate 80, and the multichannel gene sequencing reaction cell 40 is mounted on the cell installing plate 80
On, the first installation through-hole 801 is provided on the cell installing plate 80, is had in the first installation through-hole 801 of cell installing plate 80
There is opposite medial surface, and the multiple limit protrusions 802 stretched into the first installation through-hole 801 are provided on medial surface, it is each interior
Limiting gap 803 is formed between the adjacent limit protrusion 802 of side；In the present embodiment, underlying substrate 60 has upper surface and phase
To two sides, reactive tank 601 is arranged on the upper surface of underlying substrate 60, and the two sides of underlying substrate 60 are both provided with multiple limits
Position groove 6031, and form protrusion 6032 between the adjacent limiting groove 6031 of each side；The underlying substrate 60
In in the first installation through-hole 801 of cell installing plate 80, and the protrusion 6032 of underlying substrate 60 is inserted into cell installing plate 80
Limiting gap 803 in, the limit protrusion 802 of the cell installing plate 80 is inserted into the limiting groove 6031 of underlying substrate 60.
The present invention is embedded in cell installation by such structure to the underlying substrate 60 of multichannel gene sequencing reaction cell 40
In first installation through-hole 801 of plate 80, pass through matching for the limiting gap 803 of the protrusion and cell installing plate 80 of underlying substrate 60
The cooperation of the limit protrusion 802 of the limiting groove 6031 and cell installing plate 80 of conjunction and underlying substrate 60, realizes to lower floor
The positioning of substrate 60 prevents multichannel gene sequencing reaction cell 40 from occurring deviation when mounted；Also, due in underlying substrate
Need to process multiple reactive tanks 601 on 60, underlying substrate 60 should select material easy to process to be made, in the present embodiment under
The material of laminar substrate 60 is PDMS（Dimethyl silicone polymer）；When adapter 70 is pressed in multichannel gene sequencing reaction cell 40
When in top substrate layer 50, the strength that adapter 70 pushes is transferred on underlying substrate 60, at this time as underlying substrate 60 directly fits
On cell installing plate 80, then easily underlying substrate 60 is deformed upon, influence being smoothed out for sequencing reaction.And in this reality
It applies in example, the reagent inlet 401 of top substrate layer 50 accesses the inlet region 6011, and the reagent exit 402 of top substrate layer 50 connects
Entering the outlet area 6013, inlet region 6011 and outlet area 6013 are arranged on the protrusion 6032 of underlying substrate 60, and
And underlying substrate 60 protrusion 6032 be inserted into cell installing plate 80 limiting gap 803 in, when two adapters 70 be pressed in it is more
During the upper surface of the top substrate layer 50 of channel gene sequencing reaction small chamber 40, since underlying substrate 60 is arranged on cell installing plate 80
The first installation through-hole 801 in, therefore underlying substrate 60 is not affected by the pressure of adapter 70, will not generate deformation and influence to lead to more
Dao gene sequencing reaction is smoothed out, meanwhile, the lower surface of top substrate layer 50 is resisted against the limit protrusion of cell installing plate 80
On 802, limit protrusion 802 plays supporting role to top substrate layer 50, and the pressure for preventing adapter 70 is excessive, causes upper strata base
The rupture of plate 50.
In Figure 13, Figure 14 the embodiment described, further, the cell installing plate 80 is provided at both ends with limited block
804, the top substrate layer 50 of the multichannel gene sequencing reaction cell 40 is located between two limited blocks 804, limited block 804
Corner is additionally provided with chamfering, and guiding is played when multichannel gene sequencing reaction cell 40 is put on cell installing plate 80 and is made
With；In addition, locating piece 805 is additionally provided on the cell installing plate 80, when multichannel gene sequencing reaction cell 40 is mounted on
When on cell installing plate 80, the side of the top substrate layer 50 of multichannel gene sequencing reaction cell 40 is resisted against the locating piece
On 805, likewise, the corner of locating piece 805 is also equipped with chamfering, cell is put into multichannel gene sequencing reaction cell 40
Guiding role is played when on installing plate 80.
As shown in Figure 15, Figure 16, for the multichannel gene sequencing reaction device, the invention also provides an implementations
Example, on the basis of Figure 13, Figure 14 the embodiment described, multichannel gene sequencing reaction device further includes fixed seat 90 and rotation
Frame 100 is provided with the second installation through-hole 901 in the fixed seat 90, and the cell installing plate 80 is provided at both ends with screw hole
806, corresponding screw hole 902 is provided in fixed seat 90, it, will by locking screw 807 into the screw hole 806 of cell installing plate 80
Cell installing plate 80 is fixed on the lower surface of fixed seat 90, so as to which multichannel gene sequencing reaction cell 40 be made to be located at the second installation
The lower position of through-hole 901；The rotating turret 100 is provided in a shaft 1001, and shaft 1001 is provided at both ends with bearing,
And bearing is set in bearing holder (housing, cover) 1002, by the second installation through-hole 901 that bearing holder (housing, cover) 1002 is fixed on to the fixed seat 90
It is interior, rotating turret 100 is made to be rotatably installed in the second installation through-hole 901 of fixed seat 90, as shown in figure 15, when 100 court of rotating turret
After being rotated to the direction of multichannel gene sequencing reaction cell 40, rotating turret 100 is then pressed on the adapter 70, makes to turn
Joint chair 70 is tightly attached on multichannel gene sequencing reaction cell 40.
As shown in Figure 16, Figure 17, on the basis of the above embodiments, for adding for multichannel gene sequencing reaction device
Hot mode, the present invention propose a specific embodiment, the top substrate layer 50 of the multichannel gene sequencing reaction cell 40 it is upper
Surface is coated with heating coating 200, and the heating coating 200 is ITO coatings in the present embodiment；It is installed on the rotating turret 100
There is conductive thimble 300, specifically, the conductive thimble 300 is fixed on thimble pressing plate 3001, thimble pressing plate 3001 and rotation
Corresponding screw hole is provided on frame 100, by locking screw, thimble pressing plate 3001 is fixed on to the lower surface of rotating turret 100, when
After the direction of the rotating turret 100 towards multichannel gene sequencing reaction cell 40 rotates, the conduction thimble 300 is applied with heating
Layer 200 is in contact；As shown in figure 17, two groups of conduction thimbles 300 are located at the both ends of multichannel gene sequencing reaction cell 40 respectively,
After energization, electric current then flows through the heating coating 200, and heating coating 200 is then small to multichannel gene sequencing reaction after generating heat
It is heated room 40.Further, a spring-loaded plunger 1003, the bottom of spring-loaded plunger 1003 are also equipped on the rotating turret 100
Portion is installed with a temperature sensor, after direction rotation of the rotating turret 100 towards multichannel gene sequencing reaction cell 40,
Being tightly attached on the heating coating 200 of multichannel gene sequencing reaction cell 40 for temperature sensor, passes through the work of temperature sensor
With the temperature of detection multichannel gene sequencing reaction cell 40.
In addition, being equipped with a coil holder 1004 on the rotating turret 100, multiple mounting holes are provided on the coil holder 1004
1005, it connects above-mentioned conductive thimble 300, the conducting wire of temperature sensor is then penetrated in the mounting hole 1005 of coil holder 1004, passes through
The effect of coil holder 1004 avoids conducting wire from rambling situation occur.Further, it is rotatablely equipped with one on the fixed frame
Wedge-shaped limited block 1006, the rotating turret 100 towards multichannel gene sequencing reaction cell 40 direction rotation after, the wedge shape
Limited block 1006 is then pressed on the rotating turret 100, in Figure 15 the embodiment described, wedge-shaped limited block 1006 then direction
The direction rotation of multichannel gene sequencing reaction cell 40, is pressed on rotating turret 100, the lower surface of rotating turret 100 is then pressed in
On the adapter 70 of multichannel gene sequencing reaction cell 40, make closely to paste between the upper surface of adapter 70 and top substrate layer 50
It closes, avoids the occurrence of the situation of leakage.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (16)
1. a kind of multichannel gene sequencing reaction cell, it is characterised in that：Including top substrate layer, underlying substrate and a pair of of switching
Seat；It is provided with multiple reactive tanks on the underlying substrate, the top substrate layer is fitted on underlying substrate and by the reaction
Slot is closed, and the top substrate layer is provided with the reagent inlet and reagent exit being connected with reactive tank；
Be provided with the first through hole for being passed through reagent on the adapter, adapter be internally provided with a plurality of liquid separation channel and
The multiple liquid separation holes being connected with liquid separation channel, the first through hole are connected with liquid separation channel, and the first through hole with it is every
The distance between a liquid separation hole is equal；
One of adapter is close to the side of top substrate layer, and each liquid separation hole respectively with a reagent inlet of top substrate layer
It is connected；Another adapter is close to the opposite side of top substrate layer, and each liquid separation hole respectively with a reagent of top substrate layer
Outlet is connected.
2. multichannel gene sequencing reaction cell according to claim 1, it is characterised in that：The reactive tank is included successively
The width of inlet region, reaction zone and the outlet area of connection, the inlet region and outlet area is respectively less than the width of the reaction zone,
The reagent inlet access inlet region, the reagent exit access outlet area.
3. multichannel gene sequencing reaction cell according to claim 2, it is characterised in that：The underlying substrate has upper
Surface and opposite two sides, reactive tank are arranged on the upper surface of underlying substrate, and the two sides of the underlying substrate are both provided with
Multiple limiting grooves, and protrusion is formed between the adjacent limiting groove of each side, the inlet region and outlet area are set respectively
It puts on the protrusion of two sides of underlying substrate.
4. multichannel gene sequencing reaction cell according to claim 1, it is characterised in that：The adapter includes briquetting
And connector；
The connector have first surface and second surface, the liquid separation channel be arranged on it is recessed on connector first surface
Slot, the liquid separation hole is through first surface and second surface；
The briquetting is pressed on the first surface of connector, and the second surface of the connector is tightly attached to the top substrate layer
On.
5. multichannel gene sequencing reaction cell according to claim 4, it is characterised in that：One is provided on the briquetting
Fixed groove, the first through hole are arranged in fixed groove, and a connecting tube is equipped in fixed groove, the connecting tube
It is connected with first through hole.
6. multichannel gene sequencing reaction cell according to claim 4, it is characterised in that：It is set on the liquid separation channel
There is a notch, which is reagent inlet, after the briquetting is pressed on the first surface of connector, the first of the briquetting
Through-hole is connected with the reagent inlet of the connector, and the reagent inlet and the distance in each liquid separation hole are equal.
7. multichannel gene sequencing reaction cell according to claim 4, it is characterised in that：First table of the connector
Face is provided with the first liquid separation channel, the second liquid separation channel, third liquid separation channel, the 4th liquid separation channel and the 5th liquid separation channel；
The reagent inlet is set to the center of the first liquid separation channel, and one end of two the second liquid separation channels is respectively communicated with
On the both ends of the first liquid separation channel；
The other end of two the second liquid separation channels is respectively communicated in the center of two third liquid separation channels, four the 4th liquid separations
One end of channel is respectively communicated on the both ends of two third liquid separation channels；
The other end of four the 4th liquid separation channels is respectively communicated in the center of four the 5th liquid separation channels；On the connector
Eight liquid separation holes are provided with, and eight liquid separation holes are located at the both ends of four the 5th liquid separation channels respectively.
8. multichannel gene sequencing reaction cell according to claim 5, it is characterised in that：The second liquid separation channel hangs down
Directly in the first liquid separation channel, the third liquid separation channel is perpendicular to the second liquid separation channel, and the 4th liquid separation channel is perpendicular to
Three liquid separation channels, the 5th liquid separation channel is perpendicular to the 4th liquid separation channel；
Two second liquid separation channels are located at the same side of the first liquid separation channel；Four the 4th equal positions of liquid separation channel
In the same side of third liquid separation channel, and toward the first liquid separation channel.
9. multichannel gene sequencing reaction cell according to claim 4, it is characterised in that：Second table of the connector
Face is provided with multiple liquid separation cavitys, and the liquid separation hole is connected in the liquid separation cavity, and each liquid separation cavity respectively with sequencing
One reagent inlet of reaction small chamber is connected.
10. the multichannel gene sequencing reaction cell according to claim 1 or 4, it is characterised in that：The top substrate layer
Upper surface is coated with heating coating.
11. a kind of multichannel gene sequencing reaction device, it is characterised in that：Including multichannel gene sequencing reaction cell and cell
Installing plate, multichannel gene sequencing reaction cell are mounted on the cell installing plate；
There is the underlying substrate of the multichannel gene sequencing reaction cell upper surface and opposite two sides, reactive tank to be arranged on
The upper surface of underlying substrate, the two sides of the underlying substrate are both provided with multiple limiting grooves, and the adjacent limit of each side
Protrusion is formed between the groove of position；
The first installation through-hole is provided on the cell installing plate, with opposite interior in the first installation through-hole of cell installing plate
Side, and the multiple limit protrusions stretched into the first installation through-hole are provided on medial surface, the adjacent limit convex of each medial surface
Limiting gap is formed between rising；
The underlying substrate of the multichannel gene sequencing reaction cell is located in the first installation through-hole of cell installing plate, and lower floor
The protrusion of substrate is inserted into the limiting gap of cell installing plate, and the limit protrusion of the cell installing plate is inserted into underlying substrate
In limiting groove.
12. multichannel gene sequencing reaction device according to claim 11, it is characterised in that：The cell installing plate
Limited block is provided at both ends with, the top substrate layer of the multichannel gene sequencing reaction cell is located between two limited blocks；
Locating piece, the side of the top substrate layer of the multichannel gene sequencing reaction cell are additionally provided on the cell installing plate
It is resisted against on the locating piece.
13. multichannel gene sequencing reaction device according to claim 11, it is characterised in that：The multichannel gene is surveyed
Sequence reaction unit further includes fixed seat and rotating turret；
The second installation through-hole is provided in the fixed seat, the lower surface of fixed seat is fixed at the both ends of the cell installing plate,
Multichannel gene sequencing reaction cell is made to be located at the lower position of the second installation through-hole；
The rotating turret is rotatably installed in the second installation through-hole of fixed seat, and the rotating turret is anti-towards multichannel gene sequencing
The direction of cell is answered to be pressed on the adapter after rotating.
14. multichannel gene sequencing reaction device according to claim 13, it is characterised in that：It is installed on the rotating turret
Have a conductive thimble, the rotating turret towards after the direction rotation of multichannel gene sequencing reaction cell, the conduction thimble with plus
Hot coating is in contact.
15. multichannel gene sequencing reaction device according to claim 13, it is characterised in that：It is rotated on the fixed frame
One wedge-shaped limited block is installed, the rotating turret is towards after the direction rotation of multichannel gene sequencing reaction cell, the wedge shape
Limited block is then pressed on the rotating turret.
16. multichannel gene sequencing reaction device according to claim 13, it is characterised in that：It is installed on the rotating turret
There is a coil holder, multiple mounting holes are provided on the coil holder.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611263452.4A CN108265003A (en) | 2016-12-30 | 2016-12-30 | Multichannel gene sequencing reaction cell and multichannel gene sequencing reaction device |
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| CN201611263452.4A CN108265003A (en) | 2016-12-30 | 2016-12-30 | Multichannel gene sequencing reaction cell and multichannel gene sequencing reaction device |
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Application publication date: 20180710 |