CN107991263B - Cancer cell spectrum analysis device and method based on graphene terahertz source and detector - Google Patents
Cancer cell spectrum analysis device and method based on graphene terahertz source and detector Download PDFInfo
- Publication number
- CN107991263B CN107991263B CN201711454231.XA CN201711454231A CN107991263B CN 107991263 B CN107991263 B CN 107991263B CN 201711454231 A CN201711454231 A CN 201711454231A CN 107991263 B CN107991263 B CN 107991263B
- Authority
- CN
- China
- Prior art keywords
- graphene
- terahertz
- electrode
- layer
- source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 103
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 101
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 44
- 201000011510 cancer Diseases 0.000 title claims abstract description 42
- 238000010183 spectrum analysis Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 66
- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 33
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 33
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 229910052751 metal Inorganic materials 0.000 claims abstract description 26
- 239000002184 metal Substances 0.000 claims abstract description 26
- 230000005669 field effect Effects 0.000 claims abstract description 20
- 238000001228 spectrum Methods 0.000 claims abstract description 13
- 230000009471 action Effects 0.000 claims abstract description 4
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 7
- 238000004611 spectroscopical analysis Methods 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 abstract description 12
- 239000012472 biological sample Substances 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 59
- 210000004027 cell Anatomy 0.000 description 26
- 239000000463 material Substances 0.000 description 11
- 239000000126 substance Substances 0.000 description 8
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 6
- 238000011161 development Methods 0.000 description 5
- 206010027476 Metastases Diseases 0.000 description 4
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 4
- 230000009401 metastasis Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000306 recurrent effect Effects 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 229920000049 Carbon (fiber) Polymers 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 239000004917 carbon fiber Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 231100000517 death Toxicity 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 229910052763 palladium Inorganic materials 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 229910052719 titanium Inorganic materials 0.000 description 2
- 239000010936 titanium Substances 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 230000005641 tunneling Effects 0.000 description 2
- 244000186140 Asperula odorata Species 0.000 description 1
- 208000003174 Brain Neoplasms Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 235000008526 Galium odoratum Nutrition 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004883 computer application Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 238000011528 liquid biopsy Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 210000004324 lymphatic system Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 208000023983 oral cavity neoplasm Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000411 transmission spectrum Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000005740 tumor formation Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
- G01N21/3586—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation by Terahertz time domain spectroscopy [THz-TDS]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a cancer cell spectrum analysis device and method based on a graphene terahertz source and a detector. The main parts of the analysis device mainly comprise a graphene terahertz wave generation unit, a metal-medium-metal waveguide loaded with cancer cells, a graphene terahertz field effect tube detection unit and a lock-in amplifier. When high-speed electrons generated by graphene under the action of an external voltage flow through a covered silicon dioxide grating with sequentially increasing duty ratio, wide-spectrum terahertz waves are generated, the wide-spectrum terahertz waves are received by a terahertz field effect tube detection unit through a metal-medium-metal waveguide carrying cancer cells, and finally response signals of the cancer cells to the wide terahertz wave spectrum are obtained through a phase-locked amplifier. The analysis device integrates a plurality of unit devices, can be combined with a microfluidic system to efficiently perform terahertz spectrum analysis on biological samples such as cancer cells, and has wide application potential in the field of biological detection.
Description
Technical Field
The invention belongs to the technical field of terahertz waves, and relates to a cancer cell spectrum analysis device and method based on a graphene terahertz source and a detector.
Background
Terahertz (Terahertz or THz) waves generally refer to electromagnetic waves having frequencies in the range of 0.1 to 10THz, and photons of which have energies of about 1 to 10meV, which are approximately equivalent to energies of transitions between molecular vibration and rotational energy levels. Most polar molecules such as water molecules, ammonia molecules, etc. have strong absorption of THz radiation, and transitions between vibrational and rotational energy levels of many organic macromolecules (DNA, proteins, etc.) are well within the THz band. Therefore, the THz spectrum (including emission, reflection and transmission spectrums) of the substance contains abundant physical and chemical information, and the absorption and dispersion characteristics of the THz spectrum can be used for detecting and identifying chemical and biological samples such as explosives, medicines and the like, and the THz spectrum has important application value in the aspects of physics, chemistry, biomedicine, astronomy, material science, environmental science and the like.
Graphene is a hexagonal honeycomb-shaped two-dimensional material with a single-layer carbon atom thickness, and since 2004, graphene is increasingly valued by people, and has extremely wide application prospects. Graphene is the material with highest strength in the world (200 times steel), has very high heat conduction and electric conductivity (5300W/mK) (50 omega/cm) and very high specific surface area 2 630m 2 And/g, has high elasticity and high hardness (130 GPa). Graphene is highly chemically reactive, readily reacts with other chemicals to form compounds, is also capable of withstanding ionizing radiation, is lightweight, has toughness similar to carbon fibers, and has a smaller joule effect than carbon fibers. Graphene can well support surface plasmons in THz wave bands, and has many potential applications in sensing, communication and other aspects.
The spectrum analysis is mainly based on optical theory, and based on the interaction of substances and light, the correlation between the molecular structure of the substances and electromagnetic radiation is established, so that the analysis and identification of the geometrical isomerism, the stereoisomers, the conformational isomerism and the molecular structure of the substances are carried out. Spectroscopic analysis has become one of the main methods of molecular structural analysis and identification of substances in modern times. With the development of technology, technological innovations and computer applications, spectroscopic analysis has also been rapidly advancing. The spectrum analysis method has the characteristics of prominent advantages, wide application and the like, and is an indispensable tool in various scientific research and production fields. With the continuous improvement of technological development and analysis requirements, scientific researchers are also continuously innovating the spectrum analysis method. Because of its rapid, sensitive, accurate, and important role in cell detection, spectroscopy has become a common analytical tool and an important analytical method for cancer cell analysis and identification.
Cancer has become the current global 2 nd major mortality factor, with more than about 90% of deaths from malignant tumors being caused by three major causes of early detection difficulties, susceptibility to recurrent metastasis, and drug resistance, with recurrent metastasis being the leading cause of death in tumor patients. The reason for recurrent metastasis is due to the presence of cancer cells, i.e., circulating Tumor Cells (CTCs), that shed from the primary site of cancer and circulate human blood through the vascular or lymphatic system. CTCs have been shown to play an important role in a series of processes of malignant tumor formation and metastasis, being closely related to clinical stage, progression-free survival, total survival, anti-tumor drug efficacy, etc. of various tumors, and are recognized as a potential, real-time "liquid biopsy". CTCs can reflect tumor burden, predict cancer development, assist in treatment decisions, and have increasingly important diagnostic and predictive value and roles in transformation medical research, and at the same time CTCs can become new targets for new development of anticancer drugs, providing new ideas for cancer treatment. Development of a highly sensitive, highly specific detection method is currently an urgent task for CTCs from basic research to clinical application.
Research shows that tumor cells are well metabolized and the content of macromolecules such as nucleic acid is more than that of normal tissues. On the other hand, abnormal proliferation of cancerous cells causes changes in water content and water status in cells to be sensitively captured by terahertz waves. In 2002, woodward et al reported the application of terahertz spectrum imaging technology in skin cancer detection for the first time. Thereafter, the technology is tried to detect colorectal cancer, breast cancer, liver cancer, cervical cancer, oral cavity and brain tumor tissues, and preliminary results show that normal tissues and tumor tissues are obviously different.
Disclosure of Invention
The invention aims to solve the problems of narrow spectrum, overlarge equipment volume, complex operation and the like of the existing detection equipment for detecting cancer cells by using terahertz waves, and provides a compact cancer cell spectrum analysis device based on a graphene terahertz source and a detector. The device can be connected with a microfluidic system and becomes an important tool for early cancer screening and diagnosis.
In order to solve the technical problems, the invention adopts the following technical scheme:
a cancer cell spectrum analysis device based on a graphene terahertz source and a detector, comprising: the device comprises a graphene terahertz wave generation unit, a metal-medium-metal waveguide for loading cancer cells, a graphene terahertz field effect transistor detection unit and a lock-in amplifier;
the graphene terahertz wave generation unit comprises a first grid electrode, a first silicon dioxide layer, a first graphene layer, a first source electrode and a first drain electrode, wherein the bottom layer of the graphene terahertz wave generation unit is the first grid electrode, the first grid electrode is sequentially provided with the first silicon dioxide layer and the first graphene layer upwards, one side, in contact with the first graphene layer, of the first silicon dioxide layer is provided with a plurality of grooves which are arranged in parallel, the lengths of the Y-axis directions of the grooves are the same, the widths of the X-axis directions of the grooves are sequentially increased to form a grating structure, and the first source electrode and the first drain electrode are arranged on the upper part of the first graphene layer and are respectively positioned on two sides of the first graphene layer;
the metal-medium-metal waveguide comprises two metal blocks and a medium cavity for placing cancer cells, wherein the two metal blocks are respectively arranged above the first source electrode and the first drain electrode, and the medium cavity is arranged between the two metal blocks;
the graphene terahertz field effect transistor detection unit comprises a second silicon dioxide layer, a second graphene layer, a second grid electrode, a second drain electrode and a second source electrode, wherein the second silicon dioxide layer is used as a support of the second graphene layer and is positioned above the metal-medium-metal waveguide, the second silicon dioxide layer is covered with the second graphene layer, and the second grid electrode, the second drain electrode and the second source electrode are positioned above the second graphene layer;
the phase-locked amplifier is connected with the first source electrode and the second drain electrode.
Preferably, an aluminum oxide layer is further arranged between the second grid electrode of the graphene terahertz field effect tube detection unit and the second graphene layer of the graphene terahertz field effect tube detection unit, so that quantum tunneling effect is limited, and drain leakage current is avoided.
Preferably, the first source electrode and the first drain electrode are arranged in parallel with the grooves of the grating structure.
Preferably, the second drain electrode is located right above the first drain electrode, the second source electrode is located right above the first source electrode, and the second gate electrode is located right above the dielectric cavity.
Preferably, the depth of the grooves on the first silicon dioxide layer is the same.
Preferably, the first gate, the first source, the first drain, the second gate, the second drain, and the second source in the analysis device are mixed materials composed of titanium, palladium, and copper; the first silicon dioxide layer and the second silicon dioxide layer are made of silicon dioxide with low refractive index; the first graphene layer and the second graphene layer are made of single-layer graphene; the aluminum oxide layer material is high-purity aluminum oxide; the two metal blocks are made of metal aluminum.
The invention also provides a cancer cell spectrum analysis method based on the graphene terahertz source and the detector, which comprises the following steps: placing a cell to be tested in a medium cavity, externally applying a set source-drain voltage to a first drain electrode and a first source electrode, externally applying a set grid voltage to a first grid electrode, generating terahertz waves with set bandwidths when high-speed electrons generated by a first graphene layer in a graphene terahertz wave generating unit under the action of the externally applied voltage flow through a covered silicon dioxide grating with sequentially increased duty ratio, and transmitting control voltage signals as reference signals to a lock-in amplifier; the terahertz wave penetrates through cancer cells and then reaches the graphene terahertz field effect tube detection unit, a set source electrode-drain electrode voltage is externally applied to the second drain electrode and the second source electrode, a set grid electrode voltage is externally applied to the second grid electrode, when the terahertz wave irradiates on a second graphene layer in the graphene terahertz field effect tube detection unit, the carrier mobility of the second graphene layer can be changed, so that graphene channel current between the second drain electrode and the second source electrode is changed, and the current change can be amplified and measured by a phase-locked amplifier; the terahertz spectrum of the cancer cells can be finally measured by changing the source-drain voltages of the first drain electrode and the first source electrode in cooperation with scanning.
Compared with the prior art, the invention has the following beneficial effects:
1. because of the convenient tunability and the material flexibility of the graphene, the analysis device can provide a dynamic adjusting function and is easy to be operated practically.
2. The grating with the sequentially increased X-axis direction duty ratio in the terahertz source generation unit of the analysis device can generate terahertz waves with wide spectrums, and cancer cells can be easily detected.
3. The analysis device works in the terahertz wave band, and the terahertz wave band is just in the sensitive wave band of organisms, so that the analysis device has wide application potential in the field of biological detection.
4. The device has small volume and is easy to actually detect and carry.
Drawings
FIG. 1 is a cross-sectional view of a structure of a cancer cell spectrum analyzer based on a graphene terahertz source and detector of the present invention;
fig. 2 is a structural perspective view of a cancer cell spectrum analysis device based on a graphene terahertz source and a detector of the present invention.
The marks in the figure: the device comprises a 1-graphene terahertz wave generation unit, a 2-metal-medium-metal waveguide capable of carrying a metal for loading cancer cells, a 3-graphene terahertz field effect transistor detection unit, a 4-lock-in amplifier, a 5-first grid electrode, a 6-first silicon dioxide layer, a 7-first graphene layer, an 8-first drain electrode, a 9-metal block, a 10-second silicon dioxide layer, a 11-second graphene layer, a 12-second drain electrode, a 13-second grid electrode, a 14-aluminum oxide layer, a 15-second source electrode, a 16-cell to be detected, a 17-first source electrode and a 18-grating structure.
Detailed Description
The present invention is further described below in conjunction with embodiments, which are merely some, but not all embodiments of the present invention. Based on the embodiments of the present invention, other embodiments that may be used by those of ordinary skill in the art without making any inventive effort are within the scope of the present invention.
Referring to fig. 1-2, the cancer cell spectrum analysis device based on the graphene terahertz source and detector comprises a first grid electrode 5, a first silicon dioxide layer 6, a first graphene layer 7, a first source electrode 17 and a first drain electrode 8, wherein the bottom layer of a graphene terahertz wave generation unit 1 is the first grid electrode 5, the first grid electrode 5 is provided with the first silicon dioxide layer 6 and the first graphene layer 7 in turn upwards, one side of the first silicon dioxide layer 6, which is in contact with the first graphene layer 7, is provided with a plurality of grooves which are arranged in parallel, the Y-axis direction length of the grooves is the same, the X-axis direction width of the grooves is sequentially increased to form a grating structure 18, and the first source electrode 17 and the first drain electrode 8 are arranged on the upper part of the first graphene layer 7 and are respectively positioned on two sides of the first graphene layer 7; the metal-medium-metal waveguide 2 comprises two metal blocks 9 and a medium cavity for placing cancer cells, wherein the two metal blocks 9 are respectively arranged above a first source electrode 17 and a first drain electrode 8, and the medium cavity is arranged between the two metal blocks 9; the graphene terahertz field effect transistor detection unit 3 comprises a second silicon dioxide layer 10, a second graphene layer 11, a second grid electrode 13, a second drain electrode 12 and a second source electrode 15, wherein the second silicon dioxide layer 10 is used as a support of the second graphene layer 11 and is positioned above the metal-dielectric-metal waveguide 2, the second graphene layer 11 is covered on the second silicon dioxide layer 10, and the second grid electrode 13, the second drain electrode 12 and the second source electrode 15 are positioned above the second graphene layer 11; the lock-in amplifier 4 is connected with the first source electrode 17 and the second drain electrode 12.
In the above technical solution, the first source electrode 17 and the first drain electrode 8 are disposed parallel to the grooves of the grating structure 18.
In the above technical scheme, the aluminum oxide layer 14 is further arranged between the second grid electrode 13 of the graphene terahertz field-effect transistor detection unit 3 and the second graphene layer 11 of the graphene terahertz field-effect transistor detection unit 3, so that the quantum tunneling effect is limited, and the drain leakage current is avoided.
In the above technical solution, the second drain electrode 12 is located directly above the first drain electrode 8, the second source electrode 15 is located directly above the first source electrode 17, and the second gate electrode 13 is located directly above the dielectric cavity.
In the technical scheme, the depth of the grooves on the first silicon dioxide layer (6) is the same.
The invention also provides a cancer cell spectrum analysis method based on the graphene terahertz source and the detector, which comprises the following steps: placing a cell 16 to be tested in a medium cavity, applying a certain source-drain voltage to a first drain electrode 8 and a first source electrode 17, applying a certain gate voltage to a first gate electrode 5, generating terahertz waves with a certain bandwidth when high-speed electron flow generated by a first graphene layer 7 in a graphene terahertz wave generating unit 1 under the action of the applied voltage flows through a covered silicon dioxide grating with sequentially increased duty ratio, and transmitting a control voltage signal as a reference signal to a lock-in amplifier; the terahertz wave penetrates through cancer cells and then reaches the graphene terahertz field effect tube detection unit 3, a certain source-drain voltage is externally applied to the second drain electrode 12 and the second source electrode 15, a certain gate voltage is externally applied to the second gate electrode 13, when the terahertz wave irradiates the second graphene layer 11 in the graphene terahertz field effect tube detection unit 3, the carrier mobility of the second graphene layer 11 can be changed, so that the graphene channel current between the second drain electrode 12 and the second source electrode 15 is changed, and the current change can be amplified and measured by the lock-in amplifier 4; the terahertz spectrum of the cancer cells can be finally measured by changing the source-drain voltages of the first drain electrode 8 and the first source electrode 17 in cooperation with scanning.
In the analysis device, the materials of the first gate 5, the first source 17, the first drain 8, the second gate 13, the second drain 12 and the second source 15 are mixed materials composed of titanium, palladium and copper; the materials of the first silicon dioxide layer 6 and the second silicon dioxide layer 10 are silicon dioxide with low refractive index; the materials of the first graphene layer 7 and the second graphene layer 11 are single-layer graphene; the material of the aluminum oxide layer 14 is high-purity aluminum oxide; the two metal blocks 9 are made of metal aluminum.
Claims (4)
1. A cancer cell spectrum analysis device based on a graphene terahertz source and a detector, characterized by comprising: the device comprises a graphene terahertz wave generation unit (1), a metal-medium-metal waveguide (2) for loading cancer cells, a graphene terahertz field effect transistor detection unit (3) and a lock-in amplifier (4);
the graphene terahertz wave generation unit (1) comprises a first grid (5), a first silicon dioxide layer (6), a first graphene layer (7), a first source electrode (17) and a first drain electrode (8), wherein the bottom layer of the graphene terahertz wave generation unit (1) is the first grid (5), the first grid (5) is sequentially provided with the first silicon dioxide layer (6) and the first graphene layer (7) upwards, one side of the first silicon dioxide layer (6) contacted with the first graphene layer (7) is provided with a plurality of grooves which are arranged in parallel, the lengths of Y-axis directions of the grooves are the same, the widths of X-axis directions of the grooves are sequentially increased to form a grating structure (18), and the first source electrode (17) and the first drain electrode (8) are arranged on the upper part of the first graphene layer (7) and are respectively positioned on two sides of the first graphene layer (7);
the metal-medium-metal waveguide (2) comprises two metal blocks (9) and a medium cavity for placing cancer cells, wherein the two metal blocks (9) are respectively arranged above a first source electrode (17) and a first drain electrode (8), and the medium cavity is arranged between the two metal blocks (9);
the graphene terahertz field effect transistor detection unit (3) comprises a second silicon dioxide layer (10), a second graphene layer (11), a second grid electrode (13), a second drain electrode (12) and a second source electrode (15), wherein the second silicon dioxide layer (10) is used as a support of the second graphene layer (11) and is positioned above the metal-medium-metal waveguide (2), the second graphene layer (11) is covered on the second silicon dioxide layer (10), and the second grid electrode (13), the second drain electrode (12) and the second source electrode (15) are positioned above the second graphene layer (11);
the phase-locked amplifier (4) is connected with the first source electrode (17) and the second drain electrode (12);
an aluminum oxide layer (14) is further arranged between the second grid electrode (13) of the graphene terahertz field-effect tube detection unit (3) and the second graphene layer (11) of the graphene terahertz field-effect tube detection unit (3); the first source electrode (17) and the first drain electrode (8) are arranged in parallel with the grooves of the grating structure (18).
2. The cancer cell spectrum analysis device based on a graphene terahertz source and a detector as claimed in claim 1, wherein: the second drain electrode (12) is positioned right above the first drain electrode (8), the second source electrode (15) is positioned right above the first source electrode (17), and the second grid electrode (13) is positioned right above the medium cavity.
3. The cancer cell spectrum analysis device based on a graphene terahertz source and a detector as claimed in claim 1, wherein: the depth of the grooves on the first silicon dioxide layer (6) is the same.
4. A method of cancer cell spectroscopy based on a graphene terahertz source and detector according to the apparatus of claim 1, characterized by comprising the steps of: placing a cell (16) to be detected in a medium cavity, externally applying a set source-drain voltage to a first drain electrode (8) and a first source electrode (17), externally applying a set grid voltage to a first grid electrode (5), generating terahertz waves with a set bandwidth when high-speed electron flows generated by a first graphene layer (7) in a graphene terahertz wave generating unit (1) under the action of the externally applied voltage and flows through a covered silicon dioxide grating with sequentially increasing duty ratio, and transmitting a control voltage signal as a reference signal to a lock-in amplifier; the terahertz waves penetrate through cancer cells and then reach the graphene terahertz field effect tube detection unit (3), a set source-drain voltage is externally applied to the second drain electrode (12) and the second source electrode (15), a set grid voltage is externally applied to the second grid electrode (13), when the terahertz waves irradiate the second graphene layer (11) in the graphene terahertz field effect tube detection unit (3), the carrier mobility of the second graphene layer (11) is changed, and therefore graphene channel current between the second drain electrode (12) and the second source electrode (15) is changed, and the current change can be amplified and measured by the lock-in amplifier (4); the terahertz spectrum of the cancer cells can be finally measured by changing the source-drain voltage of the first drain electrode (8) and the first source electrode (17) in cooperation with scanning.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711454231.XA CN107991263B (en) | 2017-12-28 | 2017-12-28 | Cancer cell spectrum analysis device and method based on graphene terahertz source and detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711454231.XA CN107991263B (en) | 2017-12-28 | 2017-12-28 | Cancer cell spectrum analysis device and method based on graphene terahertz source and detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107991263A CN107991263A (en) | 2018-05-04 |
| CN107991263B true CN107991263B (en) | 2023-09-22 |
Family
ID=62043105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711454231.XA Active CN107991263B (en) | 2017-12-28 | 2017-12-28 | Cancer cell spectrum analysis device and method based on graphene terahertz source and detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107991263B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109765178B (en) | 2018-05-30 | 2020-04-21 | 京东方科技集团股份有限公司 | Microfluidic device, driving method and microfluidic detection system |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4759770B2 (en) * | 2009-04-16 | 2011-08-31 | ナルックス株式会社 | Terahertz electromagnetic wave generating element |
| CN103117316A (en) * | 2013-01-30 | 2013-05-22 | 中国科学院苏州纳米技术与纳米仿生研究所 | Graphene transistor based on metamaterial structure, optical sensor based on metamaterial structure, and application of graphene transistor |
| CN104466617A (en) * | 2013-09-18 | 2015-03-25 | 中国科学院苏州纳米技术与纳米仿生研究所 | Terahertz light source chip and manufacturing method thereof, terahertz light source device and manufacturing method thereof, and terahertz light source module and manufacturing method thereof |
| CN104795410A (en) * | 2015-04-15 | 2015-07-22 | 重庆大学 | Graphene nanoribbon array terahertz sensor based on optical waveguide |
| CN104916732A (en) * | 2014-03-12 | 2015-09-16 | 中国科学院苏州纳米技术与纳米仿生研究所 | Graphene terahertz wave detector and manufacturing method thereof |
| CN206804521U (en) * | 2016-12-08 | 2017-12-26 | 中国计量大学 | Utilize the alcohol concentration measurement apparatus of graphene Terahertz surface plasma effect |
| CN207650100U (en) * | 2017-12-28 | 2018-07-24 | 中国计量大学 | Cancer cell Spectrum Analysis device based on graphene THz source and detector |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5473616B2 (en) * | 2009-02-09 | 2014-04-16 | 独立行政法人理化学研究所 | Terahertz electromagnetic wave detection device and detection method thereof |
| EP2602821B1 (en) * | 2011-12-07 | 2014-02-12 | Universität Augsburg | Graphene-based nanodevices for terahertz electronics |
| KR101979245B1 (en) * | 2012-12-27 | 2019-08-28 | 한국전자통신연구원 | apparatus for generating/detecting THz wave using the grapnene and manufacturing method of the same |
-
2017
- 2017-12-28 CN CN201711454231.XA patent/CN107991263B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4759770B2 (en) * | 2009-04-16 | 2011-08-31 | ナルックス株式会社 | Terahertz electromagnetic wave generating element |
| CN103117316A (en) * | 2013-01-30 | 2013-05-22 | 中国科学院苏州纳米技术与纳米仿生研究所 | Graphene transistor based on metamaterial structure, optical sensor based on metamaterial structure, and application of graphene transistor |
| CN104466617A (en) * | 2013-09-18 | 2015-03-25 | 中国科学院苏州纳米技术与纳米仿生研究所 | Terahertz light source chip and manufacturing method thereof, terahertz light source device and manufacturing method thereof, and terahertz light source module and manufacturing method thereof |
| CN104916732A (en) * | 2014-03-12 | 2015-09-16 | 中国科学院苏州纳米技术与纳米仿生研究所 | Graphene terahertz wave detector and manufacturing method thereof |
| CN104795410A (en) * | 2015-04-15 | 2015-07-22 | 重庆大学 | Graphene nanoribbon array terahertz sensor based on optical waveguide |
| CN206804521U (en) * | 2016-12-08 | 2017-12-26 | 中国计量大学 | Utilize the alcohol concentration measurement apparatus of graphene Terahertz surface plasma effect |
| CN207650100U (en) * | 2017-12-28 | 2018-07-24 | 中国计量大学 | Cancer cell Spectrum Analysis device based on graphene THz source and detector |
Non-Patent Citations (5)
| Title |
|---|
| Binggang Xiao et al..Metal-loaded graphene surface plasmon waveguides working in the terahertz regime.《OpticsCommunications》.2015,第602-606页. * |
| Out-of-plane focusing and manipulation of terahertz beams based on a silicon/copper grating covered by monolayer graphene;JIANLI JIANG;《OPTICS EXPRESS》;第25卷(第14期);全文 * |
| Xiangjun Li et al..Design of terahertz metal-dielectric-metal waveguide with microfluidic sensing stub.《OpticsCommunications》.2016,第130-137页. * |
| 基于金属光栅的氨基酸溶液太赫兹光谱检测;吴英 等;《光子学报》;第45卷(第7期);全文 * |
| 石墨烯光栅太赫兹透射特性的研究;李彤彤 等;《激光与红外》;第45卷(第11期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107991263A (en) | 2018-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ember et al. | Raman spectroscopy and regenerative medicine: a review | |
| Cutshaw et al. | The emerging role of Raman spectroscopy as an omics approach for metabolic profiling and biomarker detection toward precision medicine | |
| Kast et al. | Emerging technology: applications of Raman spectroscopy for prostate cancer | |
| CN207650100U (en) | Cancer cell Spectrum Analysis device based on graphene THz source and detector | |
| Constantinou et al. | Label-free sensing with metal nanostructure-based surface-enhanced Raman spectroscopy for cancer diagnosis | |
| Zhang et al. | A miniaturized photoacoustic device with laptop readout for point-of-care testing of blood glucose | |
| Zhan et al. | Revolutionary approaches for cancer diagnosis by terahertz-based spectroscopy and imaging | |
| Dina et al. | Fuzzy characterization and classification of bacteria species detected at single-cell level by surface-enhanced Raman scattering | |
| CN107991263B (en) | Cancer cell spectrum analysis device and method based on graphene terahertz source and detector | |
| Chen et al. | Label-free techniques for laboratory medicine applications | |
| CN105606585B (en) | A kind of exhalation sensor and preparation method thereof | |
| Kumar et al. | Highly stable and reproducible Au nanorod arrays for near-infrared optofluidic SERS sensor | |
| Chakraborty et al. | Recent field effect transistors and electrical impedance spectroscopy based biosensing strategies for cancer biomarker screening: A mini review | |
| Wu et al. | Recent advances in Raman spectroscopy for skin diagnosis | |
| Liu et al. | NIR-II-Absorbing TMB Derivative for 1064 nm-Excited Photothermal Immunoassay | |
| Malhotra et al. | Terahertz technology for biomedical application | |
| CN109580584A (en) | The preparation method of saliva diagnostic sensor and the application of saliva diagnostic sensor | |
| Adamczyk et al. | Stimulated Raman scattering microscopy in the analysis of cancer cells–a review and own research | |
| Thomas et al. | The use of Raman spectroscopy in cancer diagnostics | |
| Vaks et al. | Methods and instruments of high-resolution transient THz spectroscopy for diagnostics of socially important diseases | |
| Liao et al. | Quantifying efficiency of remote excitation for surface-enhanced Raman spectroscopy in molecular junctions | |
| Khosroshahi et al. | Non-invasive optical characterization and detection of CA 15–3 breast cancer biomarker in blood serum using monoclonal antibody-conjugated gold nanourchin and surface-enhanced Raman scattering | |
| Vaks et al. | High-precision terahertz spectroscopy for noninvasive medicine diagnostics | |
| Mohanty et al. | Terahertz waves in biomedicine: Pioneering imaging and sensing for healthcare revolution | |
| Wan et al. | Point-of-Care Detection of HER2 and CA 15-3 in Breast Cancer Patients: Dual-Channel Biosensor Implementation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |