CN106771124A - A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card - Google Patents
A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card Download PDFInfo
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- CN106771124A CN106771124A CN201611037176.XA CN201611037176A CN106771124A CN 106771124 A CN106771124 A CN 106771124A CN 201611037176 A CN201611037176 A CN 201611037176A CN 106771124 A CN106771124 A CN 106771124A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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Abstract
The invention discloses a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card,Including detection card body,Detection casing clamping body,Test paper and base plate,The detection card body is provided with detection casing clamping body and Test paper,The detection casing clamping body is provided with spacing frid,Described spacing frid one end is provided with deck,The spacing frid bottom is provided with support,Base plate is fixed with the support,The base plate top is fixed with Test paper,Described Test paper one end is provided with adsorptive pads,The Test paper other end is provided with sample pad,The sample pad end superposition is provided with fluorescein pad,The fluorescein pad superposition is connected to nitrocellulose filter top,Nitrocellulose filter between the fluorescein pad and adsorptive pads forms detection zone,Nature controlling line opposite side is provided near adsorptive pads side be provided with detection line on the nitrocellulose filter.The invention possesses the simpler convenience of operation, and it is convenient as a result to read, and testing result stability is strong.
Description
Technical field
The present invention relates to technical field of biological, specially a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection examination
Paper and detection card.
Background technology
Platelet-activating factor acetylhydro-lase（Lp-PLA2）Relative molecular mass is about 45400, is phospholipase A2 superfamily
In a member, contain 441 amino acid residues.Human plasma Lp-PLA2 is by macrophage, lymphocyte and mast cell
Synthesis and secretion, the effect with pro-atherogenic can be adjusted by inflammatory mediator, be a kind of new inflammatory reaction mark
Will thing, effect is played in several Main Stages of AS.Lp-PLA2 is main to be produced by macrophage and lymphocyte,
In the research on Lp-PLA2 and AS of early stage, because Lp-PLA2 can hydrolyze pro-inflammatory cytokine such as platelet activating factor and structure
Related oxidized phospholipids, therefore suppression inflammatory reaction was once considered to, or even the formation of atherosclerosis can be suppressed, therefore
It is referred to as platelet-activating factor acetylhydrolase.But it is to promote that research in recent years has proven to the bigger effects of Lp-PLA2
Enter the generation and development of AS.Lp-PLA2 is by hydrolyzing the oxidation lecithin on the LDL on endarterium, so as to generate haemolysis ovum
Phosphatide and free oxidation of fatty acids, the latter two are pro-inflammatory mediators, can stimulate the generation of adhesion factor and cell factor, so as to lead
Monocyte is caused to be assembled from tube chamber to inner membrance.It is macrophage, macrophage phagocytic that monocyte is derivative after inner membrance aggregation
OxLDL becomes the foam cells of apoptosis, and the macrophage and foam cells of these activation can produce more Lp-PLA2 to return to
Into circulating.The foam cells of apoptosis is gathered into atherosclerotic plaques, and patch can discharge cell factor and protease, drop
The smooth muscle cell and collagen stroma of fibrous cap are solved, patch is become fragile, rupture, so as to cause the generation and development of AS, led
Cause the generation of thrombosis and cardiovascular event.
Reagent currently used for detection Lp-PLA2 is mainly the product of enzyme linked immunosorbent assay principle, with antigen-antibody
The principle of specific binding is detected that detect that product is present cumbersome, data redundancy is poor, expensive, and stability is poor
The shortcomings of.
The content of the invention
It is an object of the invention to provide a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card, tool
The standby simpler convenience of operation, testing result reads and more facilitates, and the advantage that testing result is reproducible, stability is strong, solution
Traditional detection of having determined product exists cumbersome, and data redundancy is poor, expensive, the shortcomings of stability difference.Problem.
To achieve the above object, the present invention provides following technical scheme：A kind of platelet-activating factor acetylhydro-lase fluorescent quantitation
Test paper and detection card, including detection card body, detect casing clamping body, Test paper and base plate, and the detection card body is provided with
Detection casing clamping body and Test paper, the detection casing clamping body are provided with spacing frid, and described spacing frid one end is provided with deck, described
Spacing frid bottom is provided with support, and base plate is fixed with the support, and the base plate top is fixed with Test paper, the detection
Test paper one end is provided with adsorptive pads, and the Test paper other end is provided with sample pad, and the sample pad end superposition is provided with fluorescein
Pad, fluorescein pad superposition is connected to nitrocellulose filter top, the fluorescein pad and adsorptive pads it
Between nitrocellulose filter form detection zone, be provided with nature controlling line opposite side and set near adsorptive pads side on the nitrocellulose filter
There is detection line.
Preferably, the detection casing clamping body is provided with well and observation panel.
Preferably, the well is arranged on sample pad vertical direction, and the observation panel is arranged on the fluorescein and combines
Nitrocellulose filter between pad and adsorptive pads forms detection zone top.
Preferably, the lipoprotein phospholipase A2 monoclonal that biotin labeling is fixed with the fluorescent marker pad resists
The fluorescin of body and marked by streptavidin.
Preferably, identification lipoprotein phospholipase A2 another table is fixed with the nitrocellulose filter in the detection zone
The nature controlling line that the detection line and sheep anti-mouse igg polyclonal antibody that the monoclonal antibody of position is constituted are constituted.
Compared with prior art, beneficial effects of the present invention are as follows：
1st, the present invention detects casing clamping body and Test paper by setting, and operates simpler convenience, testing result to read more
It is convenient, and testing result is reproducible, stability is strong.Detection card compact, is convenient for carrying, and facilitates testing staff to carry out original position
Detection, and during detection, without using special detection reagent and detecting instrument, it is only necessary to which detection sample is added drop-wise into well can
To be detected, testing result can read in 3-5 minutes, more solve detection time.
Brief description of the drawings
Fig. 1 is Test paper structural representation of the present invention；
Fig. 2 is structured testing casing clamping body schematic diagram of the present invention.
In figure：1- detects card body, 11- detection casing clamping bodies, 111- decks, the spacing board slots of 112-, 113- supports, 114-
Observation panel, 115- wells, 12- Test papers, 121- sample pads, 122- fluorescein pads, 123- nitrocellulose filters,
124- detection lines, 125- nature controlling lines, 126- adsorptive pads, 13- base plates.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-2 is referred to, the present invention provides a kind of technical scheme：A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection
Test paper and detection card, including detection card body 1, detect casing clamping body 11, Test paper 12 and base plate 13, the detection card body 1
Detection casing clamping body 11 and Test paper 12 are provided with, the detection casing clamping body 11 is provided with spacing frid 112, the spacing frid 112
One end is provided with deck 111, and the bottom of spacing frid 112 is provided with support 113, base plate 13 is fixed with the support 113, described
The top of base plate 13 is fixed with Test paper 12, and the one end of the Test paper 12 is provided with adsorptive pads 126, and the Test paper 126 is another
One end is provided with sample pad 121, and the superposition of the end of the sample pad 121 is provided with fluorescein pad 122, the fluorescein pad
122 superpositions are connected to the top of nitrocellulose filter 123, the cellulose nitrate between the fluorescein pad 122 and adsorptive pads 126
Plain film 123 forms detection zone, and being provided with the opposite side of nature controlling line 125 near the side of adsorptive pads 126 on the nitrocellulose filter 123 sets
There is detection line 124.
The detection casing clamping body 11 is provided with well 115 and observation panel 114；The well 115 is arranged on sample pad
121 vertical directions, the observation panel 114 is arranged on the nitrocellulose between the fluorescein pad 123 and adsorptive pads 126
Film 123 forms detection zone top；The lipoprotein phospholipase A2 of biotin labeling is fixed with the fluorescent marker pad 122
The fluorescin of monoclonal antibody and marked by streptavidin；Identification is fixed with nitrocellulose filter 23 in the detection zone
Detection line 124 and sheep anti-mouse igg polyclonal antibody that the monoclonal antibody of lipoprotein phospholipase A2 another epitope is constituted
The nature controlling line 125 of composition.
Operation principle：The present invention detects casing clamping body 11 and Test paper 12 by setting, and operates simpler convenience, inspection
Survey result and read and more facilitate, and testing result is reproducible, stability is strong.Detection card compact, is convenient for carrying, convenient inspection
Survey personnel carry out in situ detection, and during detection, without using special detection reagent and detecting instrument, it is only necessary to sample drop will be detected
It is added to well 115 and can be carried out detection, testing result can read in 3-5 minutes, more solve detection time.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (5)
1. a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection blocks, including detection card body（1）, detection card
Housing（11）, Test paper（12）And base plate（13）, it is characterised in that：The detection card body（1）It is provided with detection casing clamping body
（11）And Test paper（12）, the detection casing clamping body（11）It is provided with spacing frid（112）, the spacing frid（112）One end
It is provided with deck（111）, the spacing frid（112）Bottom is provided with support（113）, the support（113）On be fixed with base plate
（13）, the base plate（13）Top is fixed with Test paper（12）, the Test paper（12）One end is provided with adsorptive pads（126）,
The Test paper（126）The other end is provided with sample pad（121）, the sample pad（121）End superposition is provided with fluorescein combination
Pad（122）, the fluorescein pad（122）Superposition is connected to nitrocellulose filter（123）Top, the fluorescein pad
（122）With adsorptive pads（126）Between nitrocellulose filter（123）Form detection zone, the nitrocellulose filter（123）On lean on
Nearly adsorptive pads（126）Side is provided with nature controlling line（125）Opposite side is provided with detection line（124）.
2. a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper according to claim 1 and detection blocks, and it is special
Levy and be：The detection casing clamping body（11）It is provided with well（115）And observation panel（114）.
3. a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper according to claim 1 and detection blocks, and it is special
Levy and be：The well（115）It is arranged on sample pad（121）Vertical direction, the observation panel（114）It is arranged on the fluorescence
Plain pad（123）With adsorptive pads（126）Between nitrocellulose filter（123）Form detection zone top.
4. a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper according to claim 1 and detection blocks, and it is special
Levy and be：The fluorescent marker pad（122）On be fixed with the lipoprotein phospholipase A2 monoclonal antibody of biotin labeling
With the fluorescin of marked by streptavidin.
5. a kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper according to claim 1 and detection blocks, and it is special
Levy and be：Nitrocellulose filter in the detection zone（123）On be fixed with identification lipoprotein phospholipase A2 another epitope
Monoclonal antibody constitute detection line（124）The nature controlling line constituted with sheep anti-mouse igg polyclonal antibody（125）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611037176.XA CN106771124A (en) | 2016-11-23 | 2016-11-23 | A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611037176.XA CN106771124A (en) | 2016-11-23 | 2016-11-23 | A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card |
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| Publication Number | Publication Date |
|---|---|
| CN106771124A true CN106771124A (en) | 2017-05-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611037176.XA Pending CN106771124A (en) | 2016-11-23 | 2016-11-23 | A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card |
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| CN (1) | CN106771124A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019148754A1 (en) * | 2018-01-30 | 2019-08-08 | 深圳市伯劳特生物制品有限公司 | Test strip and testing method for pla2r antibody |
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| CN204330781U (en) * | 2014-10-28 | 2015-05-13 | 广州天宝颂原生物科技开发有限公司 | Lipoprotein phospholipase A2 immunochromatographiassay assay quantitative detection test paper |
| CN105486849A (en) * | 2016-01-21 | 2016-04-13 | 浙江东方基因生物制品有限公司 | Multifunctional test pen |
| CN205317783U (en) * | 2016-01-27 | 2016-06-15 | 唐小龙 | Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card |
| CN205538972U (en) * | 2016-01-21 | 2016-08-31 | 浙江东方基因生物制品有限公司 | Many functional test pen |
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2016
- 2016-11-23 CN CN201611037176.XA patent/CN106771124A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203069586U (en) * | 2013-02-20 | 2013-07-17 | 张继红 | Pull-out urine detection test paper box |
| CN204330781U (en) * | 2014-10-28 | 2015-05-13 | 广州天宝颂原生物科技开发有限公司 | Lipoprotein phospholipase A2 immunochromatographiassay assay quantitative detection test paper |
| CN105486849A (en) * | 2016-01-21 | 2016-04-13 | 浙江东方基因生物制品有限公司 | Multifunctional test pen |
| CN205538972U (en) * | 2016-01-21 | 2016-08-31 | 浙江东方基因生物制品有限公司 | Many functional test pen |
| CN205317783U (en) * | 2016-01-27 | 2016-06-15 | 唐小龙 | Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019148754A1 (en) * | 2018-01-30 | 2019-08-08 | 深圳市伯劳特生物制品有限公司 | Test strip and testing method for pla2r antibody |
| CN111413506A (en) * | 2018-01-30 | 2020-07-14 | 深圳市伯劳特生物制品有限公司 | Application of detection test strip in preparation of kit for detecting P L A2R antibody |
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Application publication date: 20170531 |
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