CN106596478A - Method of utilizing InP/ZnS QDs probe to detect dimethoate concentration - Google Patents
Method of utilizing InP/ZnS QDs probe to detect dimethoate concentration Download PDFInfo
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- CN106596478A CN106596478A CN201611033254.9A CN201611033254A CN106596478A CN 106596478 A CN106596478 A CN 106596478A CN 201611033254 A CN201611033254 A CN 201611033254A CN 106596478 A CN106596478 A CN 106596478A
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- rogor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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Abstract
The invention discloses a method of utilizing an indium phosphide/zinc sulfide quantum dot (InP/ZnS QDs) probe to detect the dimethoate concentration. The method comprises the following steps: step one, preparing InP/ZnS QDs containing standard solutions with different concentrations, detecting the fluorescence strength of the standard solutions to obtain the fluorescence spectrum of the standard solutions; and step two, preparing a InP/ZnS QDs containing sample solution, detecting the fluorescence strength of the sample solution, and determining the dimethoate concentration of the sample solution through the fluorescence spectrum of the standard solutions. InP/ZnS QDs are taken as the probe and can be quenched by dimethoate, so InP/ZnS QDs are used to detect the dimethoate concentration. The detection is simple and convenient, the sensitivity is high, the detection limit is low, and thus the online in-situ rapid and sensitive detection of dimethoate concentration is realized.
Description
Technical field
The present invention relates to a kind of method of detection Rogor concentration, more particularly to it is a kind of using InP/ZnS QDs probe in detecting
The method of Rogor concentration.
Background technology
Organophosphorus pesticide (Ops) is referred to for preventing and treating phytopathy, worm, compound of the evil containing organophosphors, this kind of pesticide species
Many, drug effect is high, and purposes is wide, easily decomposes, and is generally not easy accumulation in people, animal body.The organophosphorus pesticide of domestic production is most absolutely
Count as insecticide, the parathion such as commonly used, thimet, Rogor etc..Wherein Rogor be exactly great representational organophosphorus pesticide it
One.Conventional detection pesticide method has high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrography, enzyme point both at home and abroad
Analysis technology, thin layer chromatography etc..Although this several method sensitivity is high, selectivity is strong, pesticide concentration can be accurately detected,
As operating procedure is complicated, time-consuming for detection, apparatus expensive, poor sensitivity and the shortcomings of require higher to technical staff.
In recent years, as the continuous progressive and quantum dot of quantum dot synthetic technology is in life sciences, solaode, optics
What the fields such as device were applied gos deep into, and the impact that its toxicity is produced to environment is paid close attention to by more and more people.Therefore, it is traditional
IIB-VI quantum dots such as CdTe, CdSe etc., although its technology development comparative maturity, but containing in Cd this toxic element
In shortcoming, the application in its future is greatly limited.By comparison, III-V quantum dots have relatively low toxicity, wherein InP amounts
Son puts especially prominent (not containing the toxic elements such as Cd, Hg, As, Se), and its spectral region covers visible and near infrared region (500-
850nm), and with relatively small particle size, these features are all not available for the II-VI quantum dots such as traditional CdSe
's.Excellent biocompatibility and optical property cause InP/ZnS quantum dots to be more suitable for applying in biomedical imaging etc.
Field.
The content of the invention
For the problem being previously mentioned, the invention provides a kind of side of utilization InP/ZnS QDs probe in detecting Rogor concentration
Method, step include：
Using the method for InP/ZnS QDs probe in detecting Rogor concentration, step includes：
1) prepare Rogor original solution needed for variable concentrations standard solution；
2) the InP/ZnS QDs of 50nm are added in the Rogor solution；
3) the Rogor original solution for adding InP/ZnS QDs is diluted to 3ML using normal hexane buffer solution；
4) solution after dilution is stirred 100 times, is placed in 15min at a temperature of -8 DEG C, equal-volume is obtained different
The standard solution of concentration, its concentration value are followed successively by：：0mol/L、1×10-7mol/L、5×10-7mol/L、1×10-6mol/L、5
×10-6mol/L、1×10-6mol/L；
5) fluorescence intensity of the standard solution is detected, the fluorescence spectra of the standard solution is obtained.
6) take Rogor solution to be detected, and be added thereto to the InP/ZnS QDs of 50.00nM, then buffered with normal hexane
Solution is diluted to 3mL, obtains sample solution；
7) fluorescence intensity of the sample solution is detected, the sample is determined by the fluorescence spectra of the standard solution
The concentration of Rogor in solution.
Preferred version is：Detect that the standard solution and the excitation wavelength of the sample solution fluorescence intensity are 388nm.
Preferred version is：Fluorescence intensity of the intensity of sample Rogor with standard solution is contrasted, the sample is obtained final product
The concentration of Rogor in solution.
Preferred version is：The solution after dilution is stirred using microsyringe.
Preferred version is：1 × 10 can reach to the test limit of Rogor-7mol/L。
Rogor, a kind of pesticide, sterling are white, needle-shaped crystals, and in water, dissolubility is 39 g/l (room temperature).Easily by plant
Absorb and transporting is to Herb.It is more stable in an acidic solution, hydrolyzed in alkaline solution rapidly, therefore can not be mixed with Basic pesticides
With.One of purposes kind larger compared with wide, yield in organophosphors systemic insecticide.Chemical name O, O- dimethyl-S- (N- methyl
Carbamoyl methyl) phosphorodithioate.According to the characteristic of Rogor, on the basis of many experiments, buffered using normal hexane molten
Liquid is diluted to the standard solution obtained by 3ML to the Rogor original solution for adding the InP/ZnS QDs of 50nm, detects the mark
The fluorescence intensity of quasi- solution, obtains the fluorescence spectra of the standard solution.So that utilization InP/ZnS QDs proposed by the present invention
The method of probe in detecting Rogor concentration, sensitivity is high, accuracy rate is high, time-consuming short, be capable of achieving Rogor concentration in actual sample
The rapid sensitive detection of line original position.
Description of the drawings
Fig. 1 is that the standard solution of the Rogor of variable concentrations in one embodiment of the present of invention is anti-with InP/ZnS QDs probes
Ying Hou, the fluorescence spectra obtained when excitation wavelength is 388nm.
Specific embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to description text
Word can be implemented according to this.
It should be appreciated that it is used herein such as " have ", "comprising" and " including " term do not allot one or many
The presence or addition of individual other elements or its combination.
Embodiment 1
First, set up linear relationship
As shown in figure 1, preparing the original solution of the Rogor of variable concentrations, and add the InP/ZnS of 50.00nM thereto respectively
QDs, is then diluted to 3mL with normal hexane buffer solution, prepares a series of standard solution of equal-volume variable concentrations, wherein, mark
In quasi- solution, the concentration of Rogor is followed successively by：0mol/L、1×10-7mol/L、5×10-7mol/L、1×10-6mol/L、5×10- 6mol/L、1×10-6Mol/L its numbering be respectively a, b, c, d, e, f.With spectrofluorophotometer 388nm excitation wavelength
Under, the fluorescence intensity of above-mentioned standard solution is detected respectively, fluorescence spectra as shown in Figure 1 is obtained, and is read from fluorescence spectra
Fluorescence intensity level corresponding to the standard solution of the Rogor for going out variable concentrations.Understand that the test limit of Rogor can reach 1 × 10-7mol/
L。
2nd, in sample solution Rogor concentration measure
Separately take the original solution of the Rogor of certain volume, and be added thereto to the InP/ZnS QDs of 50.00nM, then with just
Hexane buffer solution is diluted to 3mL, obtains sample solution, wherein, in sample solution, the concentration of InP/ZnS QDs is 50.00nM.
With spectrofluorophotometer under the excitation wavelength of 388nm, the fluorescence intensity of detection sample solution is molten by Rogor concentration standard
The fluorogram of liquid determines the concentration of Rogor in sample solution.
Embodiment 2
The invention provides using the method for InP/ZnS QDs probe in detecting Rogor concentration, step includes：
1) prepare variable concentrations standard solution institute Rogor original solution；
2) the InP/ZnS QDs of 50nm are added in the Rogor original solution；
3) the Rogor original solution for adding InP/ZnS QDs is diluted to 3ML using normal hexane buffer solution；
4) solution after dilution is stirred 100 times, is placed in -8 DEG C of 15min, the mark of equal-volume variable concentrations is obtained
Quasi- solution, its concentration value are followed successively by：：0mol/L、1×10-7mol/L、5×10-7mol/L、1×10-6mol/L、5×10-6mol/
L、1×10-6mol/L；
5) fluorescence intensity of the standard solution is detected, the fluorescence spectra of the standard solution is obtained.
6) take Rogor solution to be detected, and be added thereto to the InP/ZnS QDs of 50.00nM, then buffered with normal hexane
Solution is diluted to 3mL, obtains sample solution；
7) fluorescence intensity of the sample solution is detected, the sample is determined by the fluorescence spectra of the standard solution
The concentration of Rogor in solution.
It is described to detect that the standard solution and the excitation wavelength of the sample solution fluorescence intensity are 388nm.
Although embodiment of the present invention is disclosed as above, which is not restricted to listed by description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the legend with description.
Claims (5)
1. using the method for InP/ZnS QDs probe in detecting Rogor concentration, it is characterised in that step includes：
1) prepare Rogor original solution needed for variable concentrations standard solution；
2) the InP/ZnS QDs of 50nm are added in the Rogor original solution；
3) the Rogor original solution for adding InP/ZnS QDs is diluted to 3ML using normal hexane buffer solution；
4) solution after dilution is stirred 100 times, is placed in 15min at a temperature of -8 DEG C, equal-volume variable concentrations are obtained
Standard solution, its concentration value is followed successively by：0mol/L、1×10-7mol/L、5×10-7mol/L、1×10-6mol/L、5×10- 6mol/L、1×10-6mol/L；
5) fluorescence intensity of the standard solution is detected, the fluorescence spectra of the standard solution is obtained.
6) take Rogor solution to be detected, and be added thereto to the InP/ZnS QDs of 50.00nM, then use normal hexane buffer solution
3mL is diluted to, sample solution is obtained；
7) fluorescence intensity of the sample solution is detected, the sample solution is determined by the fluorescence spectra of the standard solution
The concentration of middle Rogor.
2. the method for utilization InP/ZnS QDs probe in detecting Rogor degree according to claim 1, it is characterised in that detection
The excitation wavelength of the standard solution and the sample solution fluorescence intensity is 388nm.
3. the method for utilization InP/ZnS QDs probe in detecting Rogor concentration according to claim 1, it is characterised in that will
The intensity of sample Rogor is contrasted with the fluorescence intensity of standard solution, obtains final product the concentration of Rogor in the sample solution.
4. the method for utilization InP/ZnS QDs probe in detecting Rogor concentration according to claim 1, it is characterised in that make
The solution after dilution is stirred with microsyringe.
5. the method for utilization InP/ZnS QDs probe in detecting Rogor concentration according to claim 1, it is characterised in that right
The test limit of Rogor can reach 1 × 10-7mol/L。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611033254.9A CN106596478A (en) | 2016-11-15 | 2016-11-15 | Method of utilizing InP/ZnS QDs probe to detect dimethoate concentration |
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| CN201611033254.9A CN106596478A (en) | 2016-11-15 | 2016-11-15 | Method of utilizing InP/ZnS QDs probe to detect dimethoate concentration |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114397281A (en) * | 2021-12-15 | 2022-04-26 | 北京市农林科学院信息技术研究中心 | Visual fluorescent sensor and application thereof |
Citations (3)
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| CN101514987A (en) * | 2008-07-14 | 2009-08-26 | 马义才 | System for quantitative detection of quanta dot mark test bar and detection method thereof |
| CN102849688A (en) * | 2012-09-26 | 2013-01-02 | 广西师范学院 | Fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate, and application thereof |
| CN105784660A (en) * | 2016-04-05 | 2016-07-20 | 广西师范学院 | Method for detecting concentration of horseradish peroxidase by utilizing water-soluble InP/ZnS QDs probe |
-
2016
- 2016-11-15 CN CN201611033254.9A patent/CN106596478A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514987A (en) * | 2008-07-14 | 2009-08-26 | 马义才 | System for quantitative detection of quanta dot mark test bar and detection method thereof |
| CN102849688A (en) * | 2012-09-26 | 2013-01-02 | 广西师范学院 | Fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate, and application thereof |
| CN105784660A (en) * | 2016-04-05 | 2016-07-20 | 广西师范学院 | Method for detecting concentration of horseradish peroxidase by utilizing water-soluble InP/ZnS QDs probe |
Non-Patent Citations (3)
| Title |
|---|
| VIRGILIO BRUNETTI ET AL.: "InP/ZnS as a safer alternative to CdSe/ZnS core/shell quantum dots: in vitro and in vivo toxicity assessment", 《NANOSCALE》 * |
| 熊海 等: "水溶性InP/ZnS 量子点的合成及其在指纹显现中的应用", 《化学研究》 * |
| 黄珊 等: "CdSe/ZnS量子点荧光猝灭法测定农药甲胺磷", 《湖北大学学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114397281A (en) * | 2021-12-15 | 2022-04-26 | 北京市农林科学院信息技术研究中心 | Visual fluorescent sensor and application thereof |
| CN114397281B (en) * | 2021-12-15 | 2023-09-12 | 北京市农林科学院信息技术研究中心 | Visual fluorescent sensor and application thereof |
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Application publication date: 20170426 |