CN106172513A - A kind of Limax Limax epidermal mucus adsorbs instant biologic grain compound method - Google Patents

Abstract

It is the biochemical technology comprehensively developed of the chemical composition according to several animal body surface mucus, physicochemical property and the chemical composition of the instant biologic grain of absorption, physicochemical property and plant protection technology that a kind of Limax Limax epidermal mucus adsorbs instant biologic grain compound method；Organic molecule in this biologic grain exchanges intermolecular chemical bond with the organic molecule in animal body surface mucus, substitutes the old chemical bond between mucus particle, reaches similar dissolving purpose of dissolving each other；Experiment proves: this adsorption particle is adsorbed onto on Limax, Limax epidermal mucus and makes it immovable for 1 second, and in 30 minutes, fold after dehydration occur in molten broken rete malpighii and epidermis, within 24 hours, become dry scallop, prevention effect 99.9%；This technology has reached to utilize Limax, the Limax in the radical cure of plant source biomass Prevention Processing region and has safeguarded that protecting ecology balances purpose；Abundant raw material is easy to get, with low cost, and technique is simple, without any noxious substance and chemical substance, meets environmental requirement and fruit and vegerable Tea containing traditional Chinese medicine grass food safety requirements.

Description

A kind of Limax Limax epidermal mucus adsorbs instant biologic grain compound method

It is according to several animals that technical field one Limax Limax epidermal mucus adsorbs instant biologic grain compound method The life that the chemical composition of epidermal mucus, physicochemical property and the chemical composition of the instant biologic grain of absorption, physicochemical property are comprehensively developed Materialization learns a skill and plant protection technology.

Background technology Limax and land snail field control are in physical control and chemical prevention stage at present；Physics is prevented Control means gentle, preventive effect about 80%, exist and be difficult to effect a radical cure the problems such as life and growth in nature；Chemical prevention preventive effect is notable, there is pesticide The negative effects such as residual, toxic and side effects, destruction ecosystem, pollution environment；Limax and Limax Biological control, microbial control, Biotechnology preventing and treating is now in the research experiment stage.

Summary of the invention

One, the instant biologic grain scope of application is adsorbed: the Mollusca epidermal mucus such as Limax, land snail；Misgurni anguillicaudati, Nian The Fish epidermal mucus such as fish, loud, high-pitched sound serratd edge fish, Monopteri albi, Cyprinus carpio, Anguillar japonica；Mucus, Rhizoma Dioscoreae mucus etc. in big Folium Allii fistulosi.

Two, absorption dissolution mechanism

Limax, land snail, Misgurni anguillicaudati, Silurus asotus fish, Monopteri albi, Cyprinus carpio, Anguillar japonica epidermal mucus are rising of being secreted of animal glands Block water, defend, the glycoprotein of lubrication, glycoprotein glycosyl monosaccharide is constituted D-Gal, D-glucosamine, D-half Lactose, N, O-bis-glucosamine sulfate, O-glucosamine sulfate, sialic acid, D-Glucose aldehydic acid, D-galacturonic acid, D-Yi De alduronic acid etc., are combined into long-chain polyols aldehyde molecular structure with glycosidic bond between monosaccharide, containing multiple free hydroxyls on residue Base, carboxyl, alcoholic extract hydroxyl group, acetylamino etc.；There is the protein hydrophobic district of compact structure on glycoprotein molecule surface, mainly by non-pole Property R base aminoacid be polymerized, containing methyl, isopropyl, phenylpropyl, imino group, heterocycle, first on hydrophobic amino acid residue side chain The non-polar groups such as sulfenyl；Adsorbing instant biologic grain chemical composition is D-galactose-L-rhamnose-L-arabinose-D-wood Sugar-D-MANNOSE-D-galacturonic acid-D-Glucose-D-Glucose aldehydic acid complex polysaccharide, D-galacturonic acid methyl ester height are poly-many Sugar etc., polysaccharide is to be combined into long-chain polyols aldehyde molecular structure with glycosidic bond, containing multiple hydroxyls freely, alcohol hydroxyl on residue Base, carboxylate radical, esterification carboxyl, hemiacetal hydroxyl；Mucous glycoprotein is colourless, tasteless, satiny, has mucus to glue hands silk, is difficult to It is dissolved in water；Adsorbing instant biologic grain absorption affinity strong, hygroscopicity is strong, water-swellable colloidality, satiny, has tack-free silk.

Containing multiple free hydroxyl, free alcohol on the high poly-polysaccharide molecule residue of complex polysaccharide and D-galacturonic acid methyl ester Free hydroxyl, alcoholic extract hydroxyl group in hydroxyl, with mucous glycoprotein glycosyl exchange intermolecular hydrogen bonding, similar dissolving of dissolving each other；At D-gala On the high poly-polysaccharide molecule residue of alduronic acid methyl ester, every four galacturonic acid cores just have a carboxyl to be become esterification by esterification Carboxyl, remaining carboxyl is many with calcium ion or magnesium ion is combined into presented in carboxylate in plant cell wall, and carboxylate radical is born Ion is the basic solvent that the cation to ionic compound has stronger nucleophilicity, and single-minded solvation dissolves cation, viscous Containing positively charged aminoacid in liquid glycoprotein, amino cation and carboxylate radical are combined into carboxylate freely, and this carboxylate is not It is only soluble in the bisexual protein molecule of water further emulsifying periphery；Esterification carboxyl is non-polar group, with mucous glycoprotein The exchangeization when methyl of middle hydrophobic amino acid, isopropyl, phenylpropyl, imino group, heterocycle, methyl mercapto non-polar group are in close contact Learn key, form new intermolecular hydrophobic key, similar dissolving of dissolving each other；Hemiacetal hydroxyl on polysaccharide macro-molecular residue is highly polar Conjugated group, can be carried out with the hydroxyl in mucous glycoprotein molecule, phenolic group, thiophenol group, sulfydryl, amino, carboxyl isopolarity group Association reaction, generates the nontoxic galacturonic acid glycosides being highly soluble in water, and solvation is dissolved；At D-galacturonic acid complex polysaccharide Poly-polysaccharide mixture high with D-galacturonic acid methyl ester there are hydroxyl polar group, esterification carboxyl non-polar group, galactose The highly polar conjugated group of aldehydic acid hemiacetal hydroxyl, carboxylate radical anion group, can dissolve polar group, also can dissolve nonpolar Group, can dissolve again ionic groups, reaches solvation and dissolves mucous glycoprotein purpose.

Such as: when the adsorption particle being adsorbed onto in mucus runs into the hydrone of glycoprotein molecule surface adsorption, rapid moisture absorption Becoming polysaccharide solvent, this Solvents Solventization dissolves mucous glycoprotein；

1, containing sialic acid i.e. N-acetyl-neuraminate in Limax, land snail epidermal mucus glycoprotein glycosyl, it is mucus The polar head of glycoprotein glycosyl, the carboxyl in sialic acid molecule, hydroxyl, alcoholic extract hydroxyl group are all that polar hydrophilic group is exposed in mucus Surface adsorption hydrone, the N-acetylamino in sialic acid molecule is non-polar hydrophobic groups；The chemical composition of adsorption particle is The complex polysaccharide that glycosidic bond combines, containing polar hydrophilic group and non-polar hydrophobic groups, and sialic acid molecule " similar dissolve each other ", mutually Changing intermolecular chemical bond, solvation is dissolved；

Excuse me, it is impossible to is aligned on the space of a whole page by macromolecular structure, represents many with R plus the functional group participating in reaction below Sugar macromole, represents mucous glycoprotein macromole with GP plus the functional group participating in reaction, expresses similar dissolving of dissolving each other.

R-COOH+HOOC-GP→R-COOH…HOOC-GP

Polysaccharide molecule carboxyl+sialic acid molecule carboxyl → polysaccharide ... between sialic acid recruit, hydrogen bond is similar dissolves each other

R-CH₂OH+HOCH₂-GP→R-CH₂OH…HOCH₂-GP

Polysaccharide molecule alcoholic extract hydroxyl group+sialic acid molecule alcoholic extract hydroxyl group → polysaccharide ... between sialic acid recruit, hydrogen bond is similar dissolves each other

R-OH+HO-GP→R-OH…HO-GP

Polysaccharide molecule hydroxyl+sialic acid molecule hydroxyl → polysaccharide ... between sialic acid recruit, hydrogen bond is similar dissolves each other

R-COOCH₃+CH₃CONHCH₂-GP→R-COOCH₃…CH₃CONHCH₂-GP

Polysaccharide molecule esterification carboxyl+sialic acid molecule acetylamino → polysaccharide ... hydrophobic bond phase between sialic acid recruit Seemingly dissolve each other

2, polysaccharide molecule free hydroxyl, free alcoholic extract hydroxyl group and the free hydroxyl of mucous glycoprotein glycosyl, free alcoholic extract hydroxyl group are mutual Change the intermolecular hydrogen bonding that formation of chemical bond is new, similar dissolving of dissolving each other

R-OH+HO-GP→R-OH…HO-GP

Polysaccharide molecule hydroxyl+glycoprotein glycosyl hydroxyl → polysaccharide molecule hydroxyl ... hydrogen bond between glycoprotein glycosyl hydroxyl recruit Similar dissolve each other

R-CH₂OH+HOCH₂-GP→R-CH₂OH…HOCH₂-GP

Polysaccharide molecule alcoholic extract hydroxyl group+glycoprotein glycosyl alcoholic extract hydroxyl group → polysaccharide molecule alcoholic extract hydroxyl group ... glycoprotein glycosyl alcoholic extract hydroxyl group newly divides Between son, hydrogen bond is similar dissolves each other

3, the esterification carboxyl of the high poly-polysaccharide molecule of D-galacturonic acid methyl ester is non-polar group, with mucous glycoprotein second Non-polar group N-acetylamino in acylamino-glucose forms hydrophobic bond between recruit, similar dissolving of dissolving each other

R-COOCH₃+CH₃CONHCH₂-GP→R-COOCH₃…CH₃CONHCH₂-GP

N-acetylamino → polysaccharide in polysaccharide molecule esterification carboxyl+glycoprotein acetylglucosamine ... glycoprotein newly divides Between son, hydrophobic bond is similar dissolves each other

4, the hemiacetal hydroxyl that in polysaccharide molecule, D-galacturonic acid is active and the hydroxyl on mucous glycoprotein molecule, ammonia Base, alcoholic extract hydroxyl group, the highly polar combination of carboxyl polar group, generate the galacturonic acid glycosides molecule being highly soluble in water, and solvation is dissolved

R-OH+HOCH₂-GP→R-O-CH₂-GP+H₂O

Hydroxyl on D-galacturonic acid hemiacetal hydroxyl+glycoprotein glycosyl → glycoprotein-D-galacturonic acid glycosides+water

5, the carboxylate radical anion in the high poly-polysaccharide molecule of D-galacturonic acid methyl ester is with band in mucous glycoprotein molecule just Amino cation in charge amino acid is combined into carboxylate, and the bisexual protein molecule that carboxylate is dissolved in water also emulsifying periphery is molten Yu Shui

R-COO^-H⁺+NH₃ ⁺-GP→R-COONH₂-GP+H₂↑

Carboxylate radical+glycoprotein amino amino cation → glycoprotein-polyvalent carboxylic salt+hydrogen in polysaccharide molecule

6, the esterification carboxyl of the high poly-polysaccharide molecule of D-galacturonic acid methyl ester with in mucous glycoprotein hydrophobic amino acid Non-polar group forms new intermolecular hydrophobic key, similar dissolving of dissolving each other

R-COOCH₃+CH₃-S-CH₂CH₂-GP→R-COOCH₃…CH₃-S-CH₂CH₂-GP

Polysaccharide molecule esterification carboxyl+glycoprotein hydrophobic amino acid nonpolar R base → polysaccharide ... the non-pole of glycoprotein aminoacid Between property R base recruit, hydrophobic bond is similar dissolves each other

Three, plant material is gathered and processes

1, Herba Plantaginis fruit ear and the kind fringe mid-July of immaturity fruit ear before marshy grassland harvesting vehicle, be directly loadable into nylon yarn mesh bag In, hang natural air drying in withering house until lyophilization in winter processes；Receive at marshy grassland during 20 days-JIUYUE 25 days in August Cutting Herba Plantaginis kind fringe, be directly loadable in 40 mesh nylon gauze sacks, in withering house, hanging air-dries until lyophilization in winter processes, The kind fringe washboard taking out a part air-dried rubs seed, loads to hang in parchment paper sack and deposit after removing remove impurity skin with dustpan Standby large area artificial culture, remaining plant fringe together with immaturity fruit ear Ching Ming Festival of next year before pulverize, granularity 40 mesh, load anti- Tide woven bag is deposited standby.

2, Herba Portulacae plant all can be gathered Herba Portulacae plant during June-JIUYUE, and Herba Portulacae meat of gathering autumn is plump, Extract Portulaca oleracea aerial parts with reaping hook, shake off Herba Portulacae Some seeds also field gently, tremble gently in laboratory table after taking back Hugging lower Herba Portulacae seed, collect hanging in loading parchment paper sack and air-dry, standby large area artificial culture uses；Herba Portulacae is planted Strain cuts off deadwood and rotten leaf, is placed on 40 mesh nylon gauze big drawer lower airway and air-dries process, then by broken for air-dried Herba Portulacae plant End loads in woven bag, hanging in withering house natural air drying until lyophilization in winter processes, the Ching Ming Festival of next year before pulverize, grain Spend 40 mesh, loading moisture-proof woven bag is deposited standby.

3, at the beginning of-10 months the end of month of Helianthi Receptaculum Helianthi 9, period results Helianthi removes seed Receptaculum Helianthi, and the excellent line of Receptaculum Helianthi is worn into String hanging on the reinforcing bar of withering house, natural air drying until winter lyophilization process, the Ching Ming Festival of next year before by Receptaculum Helianthi pulverize, Granularity 40 mesh, deposits standby in loading moisture-proof woven bag.

Four, instant biologic grain compound method is adsorbed

1, a kind of Limax Limax epidermal mucus adsorbs instant biologic grain, is dried granule 7.4 containing Herba Plantaginis fruit ear and kind fringe Kilogram, Herba Portulacae plant is dried granule 4.2 kilograms, and Helianthi Receptaculum Helianthi is dried granule 50 kilograms.

Detailed description of the invention

Weigh and prepare the instant biologic grain of the absorption stirred, include Herba Plantaginis fruit ear, plant fringe be dried granule 74 grams, horse Bitterroot plant is dried granule 42 grams, and Helianthi Receptaculum Helianthi is dried granule 500 grams, puts in glass basin, puts in microwave oven in batches Microwave disinfection, every basin microwave disinfection processes 4 minutes, kills mycete, antibacterial, pour dry diameter 38 cm x after taking-up into In the Stainless steel basin of high 15 centimetres.The Stainless steel basin placement covering formed objects the most again is cooled to room temperature, is then charged into one In the plastic bag being dried, moistureproof sealing is standby.

One, instant biologic grain is adsorbed to the adsorptivity of mucus and solubility experiment

Adsorb in instant biologic grain containing Herba Plantaginis fruit ear, kind fringe, Herba Portulacae plant, Helianthi Receptaculum Helianthi；Herba Plantaginis fruit ear, Kind fringe is rich in complex polysaccharide plantago mucilage, and Herba Portulacae plant is rich in complex polysaccharide, and Receptaculum Helianthi contains D-galacturonic acid methyl ester height and gathers Polysaccharide, these complex polysaccharides are all plant cell wall main components；Plant complex polysaccharide is the chain polyhydroxy combined with glycosidic bond Base aldehyde structure, the compound similar to chemical bond can occur the similar mutual solventsization of intermolecular exchange chemical bond to dissolve reaction；

1, adsorb the absorption of instant biologic grain and dissolve mucus experiment in big Folium Allii fistulosi

The satiny mucus of every plant mucilage emiocytosis is all plant complex polysaccharide；In big Folium Allii fistulosi, mucus is satiny, no Soluble in water, and adsorb the chemical composition of instant biologic grain and structure is similar, " similar dissolve each other ", intermolecular exchange chemical bond is molten Agentization is dissolved；Instant biologic grain will be adsorbed load in 40 mesh nylon yarn mesh bags, above a poster paper, shake off gauze gently Bag, makes absorption biologic grain uniformly sow and spreads out, thickness 2 millimeters-3 millimeters；Riving after taking a Folium Allii fistulosi water filling, Folium Allii fistulosi inwall produces Raw satiny mucus, blade clot, Folium Allii fistulosi inwall alignment is adsorbed instant biologic grain drawout, within 1 second, granule uniform adsorption arrives In Folium Allii fistulosi mucus, when 4 minutes, thumb and forefinger crack strip remove the adsorption particle on Folium Allii fistulosi, without mucus and lose crisp on blade Property, blade frivolous softness such as silk；Folium Allii fistulosi chlorophyll molecule is not that complex polysaccharide can not be adsorbed instant granular dissolving and peel off； Take 1 heavy wall Folium Allii fistulosi having Herba Alii fistulosi petal again to rive, blade deposits one layer 3 millimeters, 2 millimeters, 1 millimeter of band water of crystallization not etc. Silvery white translucent Herba Alii fistulosi complex polysaccharide, produce one layer of satiny Herba Alii fistulosi mucus with water punching, by Folium Allii fistulosi mucus in the face of quasi-absorption Granule spreads out, and 1 second moment completed uniform adsorption, and after 4 hours, strip removes the adsorption particle on Folium Allii fistulosi, silvery white deposition polysaccharide on blade Disappearing, blade is without fragility frivolous softness such as silk；This experiment proves: adsorb instant biologic grain to the adsorptivity of mucus and dissolving Property the purpose that reaches moment absorption, quickly thoroughly dissolve.

2, adsorb the absorption of instant biologic grain and dissolve the experiment of Rhizoma Dioscoreae mucus

Taking one section of Rhizoma Dioscoreae, remove the peel with peeling knife, Rhizoma Dioscoreae produces satiny silk mucus at once, and Rhizoma Dioscoreae is being adsorbed instant biology Rolling on granule, one layer of absorption instant granular of 1 second Rhizoma Dioscoreae mucosa surface uniform adsorption, adsorption particle runs into Rhizoma Dioscoreae mucosa surface It is sticky that abundant hydrone is expanded to colloid, goes colloid sticky with hands strip when 4 minutes, and Rhizoma Dioscoreae surface is without mucus, but part is inhaled The Plant fiber that attached granule is trickle has been embedded within inside the sarcocarp that Rhizoma Dioscoreae is fine and closely woven being desorbed, and this experiment proves: adsorb instant life Composition granule is similar to mucus to dissolve each other, and reaches moment absorption and rapidly-soluble purpose.

3, adsorb the absorption of instant biologic grain and dissolve the experiment of Misgurni anguillicaudati epidermal mucus

Misgurni anguillicaudati epidermal mucus is the glycoprotein of mucus glands secrete, be block water, defend, greasing substance；Mucilage of loach glycoprotein Molecular structure, the molecular structure of functional group's biologic grain instant to absorption complex polysaccharide, functional group are similar, " similar dissolve each other "；Will Absorption instant granular loads in 40 mesh nylon yarn mesh bags, shakes off gauze bag uniform broadcasting one layer absorption gently at flowerpot soil surface Instant granular, thickness 2 millimeters-3 millimeters；Take a fresh and alive slippery Misgurni anguillicaudati to pinch, push away sth. made by twisting Misgurni anguillicaudati epidermal mucus with thumb, do not have Any change, then scrape stingy Misgurni anguillicaudati epidermal mucus layer with fingernail, cannot wipe off at all and peel off；Take several fresh and alive slippery Misgurni anguillicaudatis to put Adsorbing on instant biologic grain to flowerpot, within 1 second, adsorption particle is adsorbed onto on Misgurni anguillicaudati epidermal mucus, and disorderly jumping due to Misgurni anguillicaudati daps, Health continuous S-shaped distortion, roll and wind up, instant for absorption biologic grain was uniformly stained with whole body in 1 minute, when 4 minutes Misgurni anguillicaudati without Method moves and more can not be desorbed, and is not only moment physical adsorption process because adsorbing between instant biologic grain and mucus, the most also It it is the chemisorbed process quickly carrying out intermolecular exchange chemical bond；Experimental investigation when 30 minutes is light at Misgurni anguillicaudati body surface thumb Touching sth. made by twisting adsorption particle, the rete malpighii of compact structure glossy clear has been dissolved into the inelastic fragility fragment of absent-mindedness with moist Adsorption particle strip down together, lose that fish skin that rete malpighii blocks water is astringent have been occurred being dehydrated back dart curly grain, put always Misgurni anguillicaudati on adsorption particle is adsorbed the strong moisture absorption of granule, straighe thinning Misgurni anguillicaudati dried fish in 24 hours；This experiment proves: inhale Attached instant biologic grain can be adsorbed onto on Misgurni anguillicaudati epidermal mucus moment so that it is cannot move and be desorbed, and rapid solution breaks Broken Misgurni anguillicaudati epidermal mucus.

4, adsorb the absorption of instant biologic grain and dissolve Silurus asotus fish, the experiment of loud, high-pitched sound serratd edge fish epidermal mucus

Silurus asotus fish, loud, high-pitched sound serratd edge fish epidermal mucus are the glycoproteins of mucus glands secrete, are to block water, defence, greasing substance；Silurus asotus fish, Loud, high-pitched sound serratd edge fish mucous glycoprotein molecular structure, functional group and the molecular structure of absorption instant biologic grain complex polysaccharide, functional group Similar, " similar dissolve each other "；Instant biologic grain will be adsorbed load in 40 mesh nylon yarn mesh bags, tremble gently above a poster paper Hugging gauze bag, instant biologic grain, thickness 2 centimetres are adsorbed in uniform broadcasting one layer；Take fresh and alive Silurus asotus fish and loud, high-pitched sound serratd edge fish, use thumb Pushing away sth. made by twisting epidermal mucus, do not have any change, then scrape stingy epidermal mucus layer with fingernail, cannot scrape at all and scratch and peel off, Silurus asotus fish is this moment The mucus glands secrete that contracts muscles goes out a large amount of mucus, Silurus asotus fish, loud, high-pitched sound serratd edge fish is put on the instant biologic grain of absorption, absorption in 1 second Biologic grain is adsorbed onto in fish maw and side mucus, and fish constantly distorts end to end overturning of winding up, and within 4 minutes, adsorption particle is equal Even it is stained with whole body, experimental investigation when 30 minutes, push away sth. made by twisting adsorption particle gently Silurus asotus fish and loud, high-pitched sound serratd edge fish body surface thumb, structure causes The rete malpighii of close glossy clear has been dissolved into the inelastic fragility fragment of absent-mindedness and has stripped down together with adsorption particle, then uses A hands strip whole body rete malpighii gently comes off together with absorption instant granular, and fish skin is astringent have been occurred being dehydrated back dart curly grain, will The Silurus asotus fish and the loud, high-pitched sound serratd edge fish that lose rete malpighii place in water, and 3 hours " Invest, Then Investigate "s lose the Silurus asotus fish of the rete malpighii that blocks water, loud, high-pitched sound serratd edge Because imbibition round as a ball stiff turn over white；Adsorbing the Silurus asotus fish on instant biologic grain heap, loud, high-pitched sound serratd edge fish, quilt in 24 hours always The firmly straight epidermis of adsorption particle strong moisture absorption back body is dried such as kraft paper；This experiment proves: adsorbing instant biologic grain can wink Between be adsorbed onto on Silurus asotus fish, loud, high-pitched sound serratd edge fish epidermal mucus so that it is cannot move again and be desorbed, and rapid solution crushes Silurus asotus fish, loud, high-pitched sound tooth Sub-fish epidermal mucus.

5, adsorb instant biologic grain and dissolve the experiment of Limax epidermal mucus

Limax epidermal mucus is the glycoprotein of mucus glands secrete, is to block water, defence, lubrication, and absorption is mobile, and breeding etc. is lived Dynamic required material, cannot survive difficult to move even one step without epidermal mucus Limax；Limax mucous glycoprotein molecular structure, official Can group similar with adsorbing the molecular structure of instant biologic grain complex polysaccharide, functional group, " similar dissolve each other "；Instant biology will be adsorbed Granule loads in 40 mesh nylon yarn mesh bags, and stromal surface and dendrobium plant root that flowerpot internal diameter 27 centimetres is loose are trembled gently Hugging and sow adsorption particle, thickness 2 millimeters-3 millimeters, the fresh and alive Limax of 4.5 centimeter length provided by Herba Dendrobii planting greenhouse is used Glass rod pushes in porcelain soupspoon gently, and abdominal foot is dialled down gently facing to absorption instant granular, and adsorption particle is adsorbed onto Limax abdomen in 1 second In foot mucus, Limax moment roll up after slowly open health, dawdling in original place, one layer of adsorption particle of abdominal foot uniform adsorption, is twisted into again Helical form is rolled, and adsorption particle is stained with whole body, and 2 minutes motionless, is drawn in circle by Limax with a nail, observes whether it moves Move and go too far；Experimental investigation when 30 minutes, Limax is still as you were in circle, and as being wrapped in the dense saliva of Limax, epidermal mucus disappears Losing, push away sth. made by twisting adsorption particle with thumb gently, moist adsorption particle comes off, and fold after dehydration, Limax has occurred in Limax epidermis Shorten surplus 3 centimetres, the more artificial Limax of rolling gently makes its body surface again be stained with adsorption particle on adsorption particle, continues to be placed on suction Observe in attached granulation mass；In 24 hours, the Limax of the adsorbed strong moisture absorption of instant granular is thoroughly dehydrated into dry scallop, and Limax body contracts Short surplus 1.4 centimetres；This experiment proves: adsorbs instant biologic grain and can be adsorbed onto moment on Limax abdominal foot and epidermal mucus thereof, Make its most immovable fraction more cannot be desorbed, and the most thoroughly dissolve Limax epidermal mucus glycoprotein；Limax is also that terrestrial is soft Body animal, with Limax fellow disciple, same to guiding principle, same to mesh, epidermal mucus is all glycoprotein, and running into the instant biologic grain of absorption equally can wink Between adsorbed and solvation dissolve.

Two, the preventing and treating Limax booth experiment of instant biologic grain is adsorbed,

In the greenhouse facility of Herba Dendrobii planting base, Wenzhou, absorption instant biologic grain preventing and treating Limax is done real between 2014 Test；Limax likes host's Herba Dendrobii, Herba Dendrobii glycosides and Herba Dendrobii aglycon to be the odoring substances inducing Limax；Limax is hidden by day and come out at night, and arrives at dusk Morning out looks for food, Herba Dendrobii of causing harm, and it is serious that the most whole canopy Herba Dendrobii Seedling is gnawed with nothing left loss；Plant at Herba Dendrobii Red bulb is first installed in booth, because Limax keeps in dark place, has strong illumination to hide back in substrate at once, and the mankind are the reddest Outer night vision goggles cannot do Prevention technology；Instant biologic grain will be adsorbed and load in 40 mesh nylon yarn mesh bags, at night 9 time about, Above Herba Dendrobii stem and root and substrate thereof, shake off gauze sack gently uniformly sow the instant biologic grain of absorption, thickness 2-3 Millimeter, junction between Herba Dendrobii stem and the overground part of root and substrate three, and 15 centimeters radius with stem as core In the range of, the instant biologic grain of careful absorption is sowed process and is not stayed any gap and dead angle, and otherwise Limax can not be effected a radical cure raw Do not stop, because some Limaxs just hide at Herba Dendrobii root, directly climb up stem along Herba Dendrobii root and climb to again look in dendrobium plant Food, non-other Limax hidden at Herba Dendrobii root takes food Herba Dendrobii and also have to can climb Herba Dendrobii stem and leaf through Herba Dendrobii root On；The instant biologic grain processing region of absorption as long as Limax is set foot on, to be just adsorbed onto Limax flat roomy for 1 second absorption instant granular Abdominal foot mucus on, just as light foot of taking off one's shoes walks to make its immovable fraction on gravel, at all cannot be close to dendrobium plant, 30 points In clock, Limax epidermal mucus can be adsorbed the dissolving of instant biologic grain solvation, within 24 hours, is thoroughly dehydrated into dry scallop；At iron sheet stone Necessary Prevention technology Limax 20 days continuously of dry measure used in former times planting area, every day every square metre, absorption instant biologic grain 30 grams sowed by needs； Potted plant Herba Dendrobii, basin internal diameter 27 cm x high 15 centimetres every day every basin need sow absorption instant biologic grain 3 grams, continuous processing 20 Reach after it to effect a radical cure the Limax in processing region；The process date was counted from process the same day, processed first day all Kuo that can lay eggs Yu one-tenth shellfish, the Limax that trophophase immaturity can not lay eggs and the Limax newly hatched the same day, every out looking for food enters treatment region Limax is all adsorbed granular absorption and processes immovable lethal, and within second day, process is exactly that the ovum produced before 16 days is newly incubated today The Limax changed, within the 3rd day, process is the new Limax of ovum hatching today produced before 15 days, and such continuous processing, after 17 days, can be laid eggs , will not lay eggs, that the Limax of new hatching is all adsorbed instant granular adsorption treatment is lethal, then continuous processing is strengthened for 3 days Radical cure and prevention；Experimental investigation result shows: in processing region, Herba Dendrobii rice shoot survives without the new insect population of Limax, new insect population rate 0； Without the check plot of any process, Herba Dendrobii Limax gnaws insect population rate 100% and Limax fecal pollution is serious；

Within 2014, Wenzhou Herba Dendrobii planting greenhouse adsorbs at instant biologic grain preventing and treating Limax experimental investigation part data Manage as follows:

Check plot Herba Dendrobii Limax insect population rate 100%, treatment region Limax new insect population rate 0；

Experiment proves: adsorb instant biologic grain processing region interior to Limax preventive effect 100%

Three, the preventing and treating Chinese cabbage Limax booth experiment of instant biologic grain is adsorbed

Limax likes host crucifer, and Limax is hidden by day and come out at night crop of causing harm；In order at Herba Dendrobii planting greenhouse Interior induction Limax, plants a few strain Chinese cabbages in growing dendrobium interval, seedbed, and result is that Limax only takes food Herba Dendrobii tender shoots branch and leaf not Taking food Chinese cabbage, but on Chinese cabbage, Limax is caused harm seriously, Chinese cabbage leaf is gnawed dressed in rags by Limax, and Limax fecal pollution is very Seriously, abundant before rare Limax resource space, also inhale daytime and do not bury on blade back, do Chinese cabbage Limax epidermal mucus absorption speed in passing Molten biologic grain adsorption treatment is tested；First stopping watering to Chinese cabbage, sultry in booth, now Chinese cabbage outer layer comes off voluntarily, does not cuts The Chinese cabbage side that disconnected prolapsus is got off, helping to take advantage of a situation by the soft Chinese cabbage without fragility, it is in disk form, by big for Chinese cabbage portion to be laid in bottom Chinese cabbage Leaflet sheet is all cut and is only left white outer leaf and Brassica campestris leaves, fresh and tender cabbage leaf release isothiocyanate abnormal smells from the patient induction Limax；Will Adsorbing instant biologic grain and load in 40 mesh nylon yarn mesh bags, gauze is shaken off on the disk outer leaf surface in Chinese cabbage prolapsus gently at dusk Sack uniformly sows the instant biologic grain of absorption, thickness 2-3 millimeter, and every strain Chinese cabbage diameter 30 cm range is carefully sowed, every strain Need every day to sow absorption instant biologic grain 3 grams；When night, Limax out took food Brassica campestris L. ssp.chinensis, it is necessary to be laid in ground through prolapsus The dish leaf disc on surface just can be climbed to above Brassica campestris L. ssp.chinensis, and adsorption particle can be adsorbed onto in Limax abdominal foot mucus moment, and Limax will be unable to Mobile fraction is lethal；In snail ovum phase 2-4 week, the needs absorption continuous Prevention technology of instant biologic grain can reach at place for 30 days Effect a radical cure and prevent and treat the purpose of Limax in reason region；The process date is within first day, to count from process；Due at Herba Dendrobii growing area Between only plant a few strain Chinese cabbage, Limax insect population rate and preventive effects can only be calculated, without any place with 3 strain strain numbers of 6 strains processed and comparison Reason is done the Chinese cabbage compareed and is gnawed dressed in rags by Limax, compares strain new insect population rate 100%, and sticky Limax fecal pollution is tight Weight, processes strain Chinese cabbage new insect population rate 0, and in Pemisetum flaccidum Griseb. diameter 30 cm range, every day can pick up many adsorption particle bags of 45-100 The Limax granule wrapped up in；Experimental investigation part data process as follows:

Experiment proves: adsorb instant biologic grain processing region Chinese cabbage Limax preventive effect 100%

Four, conclusion

The region that instant biologic grain processes, the Limax that can lay eggs, growth and development stage of will not laying eggs is adsorbed through 20 days Limax, just hatched out the Limax looked for food the same day, is all adsorbed the absorption of instant biologic grain and dissolves immovable lethal, Reach the purpose for the treatment of region radical cure Limax；As preventing and treating SARS, prevent and treat Limax enter growing dendrobium district, it is necessary to dendrobe cultivation Substrate carries out strict ovicidal process, does not make 1 Limax ovum enter treatment region, the most again to process 20 days, and Lao Min hinders Wealth；It is pure organic Chinese medicine through adsorbing Limax and the land snail of instant biologic grain adsorption treatment, tweezer after 48 hours, can be used Son is picked up, and is soaked in water 20 minutes and removes adsorption particle, loads to hang in parchment paper sack and deposits, it is also possible to directly also after air-drying Field is as Herba Dendrobii and crop biological nitrogen fertilizer；The instant biologic grain of absorption sowing soil and stromal surface is that plant source is raw Material hybrid particles, is quickly decomposed into humus as soil nutriment and direct returning to farmland under the effect of microorganism, is conducive to raw State environmental nutrient recovers and safeguards that protecting ecology balances；This technology abundant raw material is easy to get, with low cost, and technique is simple, without any Noxious substance and chemical substance, meet environmental requirement and organic fruit and vegerable, Chinese medicine, crop, flowers food safety requirements.

Claims (1)

1. Limax Limax epidermal mucus adsorbs an instant biologic grain, is dried granule 7.4 kilograms containing Herba Plantaginis fruit ear and kind fringe, Herba Portulacae plant is dried granule 4.2 kilograms, and Helianthi Receptaculum Helianthi is dried granule 50 kilograms.