CN106018784A - Small electrochemical luminescence immunoassay analyzer and analysis method thereof - Google Patents
Small electrochemical luminescence immunoassay analyzer and analysis method thereof Download PDFInfo
- Publication number
- CN106018784A CN106018784A CN201610521811.5A CN201610521811A CN106018784A CN 106018784 A CN106018784 A CN 106018784A CN 201610521811 A CN201610521811 A CN 201610521811A CN 106018784 A CN106018784 A CN 106018784A
- Authority
- CN
- China
- Prior art keywords
- unit
- sample
- hatch
- cup
- hatching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a small electrochemical luminescence immunoassay analyzer which comprises a machine frame with a platform at the bottom. The platform is provided with a special reagent unit, a sample bin unit, an incubation unit and a general reagent unit. An upper platform is arranged above the rear portion of the machine frame and provided with a mechanical arm unit. The mechanical arm unit comprises a first mechanical arm provided with a sampling needle and a second mechanical arm provided with a rear processing needle. The machine frame is further provided with a measurement and analysis unit, a liquid path unit, a displayer unit and a board card control unit, wherein the measurement and analysis unit is used for measuring and analyzing an incubated reaction sample, the liquid path unit is used for controlling the sample, the reagent and the waste liquid flow direction, the displayer unit is used for human-computer interaction, and the board card control unit is used for carrying out circuit control over the units. The small electrochemical luminescence immunoassay analyzer has the advantages that the structure is compact, the occupied area is small, the reagent can be preserved for a long time, the whole incubation process and measurement and analysis process are high in automation degree, human assistance is not needed, manual errors are reduced, the measurement precision is excellent, measurement is easy and convenient, and working efficiency is greatly improved.
Description
Technical field
The present invention relates to a kind of biological body fluid or blood sample analysis device, particularly to a kind of small electrical chemical illumination immunity analysis instrument and the method for analysis thereof.
Background technology
Electrogenerated chemiluminescent immunoassay instrument is mainly used in analyte assay in clinical blood, other body fluid samples.Chemiluminescence immune assay (CLIA) is the technology worldwide grown up over nearly 20 years, relative to immuno analytical methods such as radioimmunoassay, RIA (RIA), EIA enzyme immunoassay (EIA), fluorescence immunoassays (FIA), have environmental protection, do not affected by temperature and acid-base value, reagent long shelf-life, accuracy and sensitivity advantages of higher.Therefore chemiluminescence immunoassay is widely used in in-vitro diagnosis (IVD) field.
Chemiluminescence immunoassay kind is more, such as direct chemoluminescence method based on acridinium ester, indirect chemiluminescence method based on alkali phosphatase, Electrochemiluminescince etc..Directly chemoluminescence method and enzyme touch the luminescence process of luminescent method adds exciting liquid by instrument, and therefore fluorescent lifetime is relevant to liquid addition manner.Electrochemiluminescence process fluorescent lifetime is controlled by electrode power-up time, and therefore fluorescent lifetime concordance is good.Electrochemiluminescence apparatus measures analytic unit generally uses pipeline sample introduction to design, the open input mode of relatively direct chemiluminescence, can effectively prevent open module light leak and photomultiplier tube exposes risk under ambient light.
The common instrument using Electrogenerated chemiluminescent immunoassay method has Roche cobas E411 and E601, although these instrument test speeds are fast, automaticity is higher, but take larger space, and it is unfavorable for that the occasion of less test volume uses, and these instruments belong to import equipment, expensive high with reagent test cost, therefore in small hospital, large hospital little section office Ji Shekang center cannot obtain universal use.Existing small chemical luminescence immunoassay instrument generally uses semi-automatic design, eliminates and hatches a glass feeding processing means, incubation reaction device, Magneto separate cleaning device etc., only remains luminous measurement apparatus and control system.This quasi-instrument saves instrument cost, decreases volume, but sample-adding, reagent adding, incubation reaction process are required to manual operation, add the complexity of test, and when carrying out multiple test sample at the same time, easily cause operational error, and then have impact on test result accuracy, there is potential safety hazard.Another existing fully automatic electric chemiluminescent analyzer, structure is complicated, and volume is bigger；Existing fully automatic electric chemiluminescent analyzer, carries out low-temperature preservation to reagent is typically no, it is impossible to long period placement detects；Existing fully automatic electric chemiluminescent analyzer, owing to reaction cup entirety is delivered to its measurement apparatus by needs, unavoidable need to be designed as measurement apparatus open module, so extraneous photon easily enters in measurement apparatus, when reaction cup enters measurement apparatus the most every time, form differs, when causing measuring, the faint light subnumber obtained for the photomultiplier tube PMT of reading is because being easily subject to the impact of extraneous photon entrance, causing very large deviation occur, the certainty of measurement causing whole measurement system is the highest；The most existing fully automatic electric chemiluminescent analyzer, typically no touch display unit, does not has man-machine interactive operating system, the most convenient in operation.
Summary of the invention
It is an object of the invention to provide a kind of small electrical chemical illumination immunity analysis instrument for overcoming the deficiency of above-mentioned technology, this analyser has all automatic measurement analytic function, and volume is little, easy to use, measures efficiently and certainty of measurement is high.
The technical scheme is that and be achieved in that, this small electrical chemical illumination immunity analysis instrument, including bottom be provided with the frame of platform, described platform is provided with special agent unit, sample storehouse unit, hatches unit, common reagent unit；Described special agent unit include dedicated kit, dedicated kit mounting groove, for stirring the mixing mechanism of special agent and for the first temperature control device for cooling of low-temperature preservation dedicated kit；Described hatch unit include by hatch motor drive rotate hatch dish, hatch dish is put integrate multiple and hatch hatching glass group and hatching the temperature control heating diaphragm that dish bottom is provided with of cup；Described common reagent unit includes the second temperature control device for cooling that universal kit, universal kit mounting groove and its underpart are provided with；Described frame back upper place is provided with upper mounting plate, described upper mounting plate is provided with mechanical arm unit, described mechanical arm unit includes being provided with the first mechanical arm of sample needle and being provided with the second mechanical arm of post processing pin, described sample needle under described first mechanical arm drives can at described special agent unit, sample storehouse unit, hatch and move freely above unit, described post processing pin can move freely above unit, common reagent unit described hatching under described second mechanical arm drives；It is additionally provided with in described frame and measures analytic unit, for controlling sample and the reagent flow direction and collecting the fluid path unit of waste liquid, for carrying out the display unit of man-machine interaction and being connected to central controlled board control unit with remaining each unit circuit described.
Preferably, described first temperature control device for cooling or the second temperature control device for cooling, including can the base plate of heat conduction, described base plate is provided with refrigeration conductive plate in turn below, semiconductor refrigeration chip, fin and fan, described semiconductor refrigeration chip includes cold and hot two sides, described hot side is fitted and connected with described fin one side, described fan is connected to dispel the heat with described fin another side, described huyashi-chuuka (cold chinese-style noodles) is fitted and connected with described refrigeration conductive plate one side, described refrigeration conductive plate another side is fitted and connected with described base plate lower plane, on described base plate, plane is provided with described dedicated kit mounting groove or universal kit mounting groove.
Preferably, described dedicated kit includes the magnetic bead bottle of storing, reagent bottle in box body, box body, can fill special agent in described magnetic bead bottle, reagent bottle respectively, and described magnetic bead bottle is freely rotatable, and its underpart is provided with driving section and is exposed to described box body；Described mixing mechanism includes mixing motor, mixing driving member, and described mixing driving member is connected with the driving section of described magnetic bead bottle, and described mixing motor can drive described mixing driving member to drive described magnetic bead bottle to carry out reciprocating rotation.
Preferably, described sample storehouse unit includes that described sample service sink assembly includes sample service sink, the detergent line being connected with described fluid path unit, waste liquid discharge line for putting the sample rack of sample tube, sample rack mounting seat, sample rack positioner and sample service sink assembly.
Preferably, described hatch dish in circle box-like, internal be provided with described hatch glass one_to_one corresponding and hatch cup described in can accommodating hatch hole, described hatch dish in can put and described in four groups, hatch a glass group, described in hatch glass group and include that some on glass stand of the ring-type glass stand of quadrant and vertical sleeve hatch cup.
Preferably, described cup of hatching is provided with the cup inner chamber that can contain reactant liquor, described cup of hatching includes cup and lower cup body, described upper cup is in a tubular form, the inside and outside wall of described lower cup body receive described upper cup after in the inverted conical shape being gradually reduced and bottom transition is circular shape, described glass stand is provided with glass holder hole, and described upper cup unremovably sleeve is in described glass holder hole, and described lower cup body is exposed to described glass stand.
Preferably, described in hatch dish top and be provided with and hatch disk cover, described in hatch disk cover and be provided with adding mouth, hatch the gateway of cup group for loading and take out.
Preferably, described in hatch unit and also include hatching, with described, the code-disc that dish is coaxially disposed, described code-disc bottom is provided with code-disc zero-bit induction apparatus and code-disc rotation precision induction apparatus.
Preferably, described in hatch unit and also include hatching cleaning assembly and wash bowl, described in hatch cleaning assembly and include hatching service sink, the detergent line being connected with described fluid path unit, waste liquid discharge line, described wash bowl is used for storage system strong cleanout fluid reagent bottle.
Preferably, described first mechanical arm is three-dimensional arm, described second mechanical arm is two dimension arm, described three-dimensional arm and two dimension arm are arranged on the same X-direction guide rail that described upper mounting plate is provided with, the three-dimensional Z axis arm that can move freely along Y-direction that described three-dimensional arm is included in the three-dimensional Y-axis arm that can move freely on X-direction guide rail, installs on described three-dimensional Y-axis arm, described sample needle is parallelly mounted on described three-dimensional Z axis arm and can move freely along Z-direction；Described two dimension arm be included in can move freely on X-direction guide rail two-dimentional Y-axis arm, described two dimension Y-axis arm on hard-wired two dimension Z axis arm, described post processing pin be parallelly mounted to described two dimension Z axis arm on and can move freely along Z-direction；Described three-dimensional arm and two dimension arm motion on each movable direction are driven by respective motor and Timing Belt respectively.
Preferably, described measurement analytic unit includes for measuring the measurement assembly having hatched sample, outer package assembly, for controlling the temperature-controlling component of described measurement assembly temperature, analyzing and processing circuit, described temperature-controlling component is arranged at described outer package module outer surface, described measurement assembly lucifuge is arranged at described outer package component internal and contacts with described temperature-controlling component inner surface, described measurement assembly includes measuring cell, the photomultiplier tube being arranged over for reading fluorescence of described measuring cell, outer bottom is provided with separable suction pearl Magnet of can fitting therewith, inside is provided with positive and negative electrode, described measuring cell is also associated with feed tube and drain pipe, described feed tube and drain pipe lucifuge stretch out in described outer package assembly.
Preferably, syringe that described fluid path unit includes being connected with described sampling needle, post processing pin respectively, hydraulic pump, hydraulic valve, liquid line, and it is connected to discharge the negative pressure pump needed for waste liquid, for collecting the waste liquid tank of waste liquid with measuring unit.
Preferably, described display unit is connected with described board control unit circuit, including tangibly display screen, for installing the display screen outer housing of described display screen, display screen rotating shaft, display screen support bar and scope rack.
Preferably, described board control unit is provided with central processing element and is provided with systems soft ware, described board control unit can concentrate the action coordinating to control remaining each unit, and can carry out processing and result being sent to display unit by the signal that described measurement analytic unit records.
The invention provides the survey analysis method of a kind of small electrical chemical illumination immunity analysis instrument, including following measurement analytical procedure:
(1) preparation is measured: start analyser, by system initialization；Load measuring required special agent to the dedicated kit of special agent warehouse receipt unit, and carry out magnetic bead stirring by mixing mechanism；Common reagent needed for measuring loads to the universal kit of common reagent unit；Put into hatch the hatching in dish of unit by hatching glass group；Will be equipped with, in the sample rack of test tube loading sample storehouse unit of sample, then sample rack pushing sample storehouse unit, input catalogue number(Cat.No.) bar code；
(2) measurement is started: starting at display unit and measure button, analyser enters following all automatic measurement program；
(3) incubation step one: sample needle presses measure the item automatically from the required special agent one of dedicated kit extraction, then pass through the sample service sink assembly being provided with in sample storehouse unit sample needle is cleaned, sample in the sample tube put in rear absorption sample storehouse unit, being subsequently adding to hatch in cup group available hatches in cup, hatching the dish liquid to hatching in cup and carry out 38 DEG C of insulation incubation reaction, incubation step one is reaction timing by the systems soft ware installed in board control unit；
(4) incubation step two: after incubation step one terminates, the sample needle required special agent two of extraction from dedicated kit automatically, add completed incubation step one hatch in cup, and carry out compressing mixing, hatching the dish liquid to hatching in cup and carry out 38 DEG C of insulation incubation reaction, incubation step two is reaction timing by the systems soft ware installed in board control unit；
(5) Beads enrichment: measure analytic unit and be automatically performed measuring cell preparation before incubation step two completes；After incubation step two completes, post processing pin automatically extends into hatch draws the sample completing incubation reaction in cup, and the sample having completed incubation reaction flows in measuring cell along the pipeline being connected with post processing pin；Measuring the oscillating motor that is provided with of analytic unit and drive the fork being attached thereto, make the suction pearl Magnet arranged on fork fit to measuring cell outer bottom, complete the magnetic bead in the sample hatched by magneticaction, absorption is bottom measuring cell；Then post processing pin extracts common reagent one to measuring cell automatically, and rinses magnetic bead；Complete after magnetic bead cleans, to keep liquid in measuring cell static；Pearl Magnet clear point is inhaled in oscillating motor release；
(6) analysis is measured: in measuring cell, be provided with positive and negative electrode, positive and negative electrode is energized, shiner contained on magnetic bead in measuring cell produces fluorescence because of energising, photomultiplier tube is converted into the signal of telecommunication after receiving fluorescence, measure analytic unit to be analyzed this signal of telecommunication processing, the systems soft ware uploading to after obtaining signal value install in board control unit processes, and result is shown in display unit automatically；
(7) electrode reduction: post processing pin automatically extracts common reagent two and cleans measuring cell and pipeline thereof, reversely powers up positive and negative electrode in measuring cell, makes electrode reduce；
(8) measuring unit resets: the waste liquid produced after being measured by measuring cell is discharged in the waste liquid tank that fluid path unit is provided with, and post processing pin again extracts common reagent one and fills measuring unit associated pipe and prepare next round measurement；
(9) measurement terminates: measuring and terminate, analyser is in holding state.
Beneficial effects of the present invention is as follows:
The small electrical chemical illumination immunity analysis instrument that the present invention provides, is for small hospital, the little section office of large hospital and society's health Center.This analyser is by the sample needle on mechanical arm and mechanical arm and post processing pin, respectively sample and reagent can accurately be drawn and joined to hatch in cup and hatch, and the sample after hatching is drawn into totally enclosed measurement analytic unit and measures analysis, whole hatch, to measure analysis process automaticity high, need not human assistance, decrease personal error, easy and simple to handle, drastically increase work efficiency；The dish of hatching of this analyser is provided with temperature control heating diaphragm simulation human body temperature environment and carries out sample incubation, thus improves the accuracy of measurement result；This analyser special agent and common reagent use low-temperature preservation, can be placed in instrument for a long time and measure at any time；This analyser is measured analytic unit and is used totally-enclosed and pipeline sample introduction, effectively prevent open module light leak and photomultiplier tube exposes risk under ambient light；This analyser uses electrochemiluminescence reaction principle, has the certainty of measurement of excellence, can realize the immunization program huge number analyzed；The touch display screen that this analyser is provided with can directly carry out man-machine interaction, simple to operation, contributes to the realization of full-automation.
Accompanying drawing explanation
Fig. 1 is the complete machine structure figure one of the embodiment of the present invention；
Fig. 2 is the complete machine structure figure two of the embodiment of the present invention
Fig. 3 is the special agent cellular construction figure one of the embodiment of the present invention；
Fig. 4 is the structure chart of the dedicated kit of the embodiment of the present invention；
Fig. 5 be the embodiment of the present invention dedicated kit in the three-dimensional view of magnetic bead bottle；
Fig. 6 be the embodiment of the present invention dedicated kit in the three-dimensional view of reagent bottle；
Fig. 7 is the special agent cellular construction figure two of the embodiment of the present invention；
Fig. 8 is the special agent cellular construction figure three of the embodiment of the present invention；
Fig. 9 is the sectional view of the first temperature control device for cooling of the embodiment of the present invention；
Figure 10 is the sample storehouse cellular construction figure of the embodiment of the present invention；
Figure 11 is the sample rack structure chart of the sample storehouse unit of the embodiment of the present invention；
Figure 12 be the embodiment of the present invention hatch cellular construction figure one；
Figure 13 be the embodiment of the present invention hatch cellular construction figure two；
Figure 14 be the embodiment of the present invention hatch cellular construction figure three；
Figure 15 be the embodiment of the present invention hatch unit hatch cup group structure chart；
Figure 16 be the embodiment of the present invention hatch unit hatch cup group STRUCTURE DECOMPOSITION figure；
Figure 17 is the common reagent cellular construction figure of the embodiment of the present invention；
Figure 18 is the mechanical arm cellular construction figure of the embodiment of the present invention；
Figure 19 is the display cell structure figure of the embodiment of the present invention；
Figure 20 is the measurement analytic unit structure chart of the embodiment of the present invention；
Figure 21 is the measurement analytic unit STRUCTURE DECOMPOSITION figure of the embodiment of the present invention；
Figure 22 is the measurement assembly assumption diagram measuring analytic unit of the embodiment of the present invention；
Figure 23 is the fluid path cellular construction figure of the embodiment of the present invention；
Figure 24 is the measurement analytical procedure flow chart of the embodiment of the present invention.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.
As shown in Figures 1 and 2, the embodiment of small electrical chemical illumination immunity analysis instrument of the present invention, including frame 1, frame 1 includes the platform 11 that bottom is provided with, and the front of platform 11 has been arranged side by side special agent unit 2, sample storehouse unit 3 successively, has hatched unit 4, common reagent unit 5；The back upper place of frame 1 or platform 11 is provided with upper mounting plate 12, and upper mounting plate 12 is provided with mechanical arm unit 6；The side, front of platform 11 is provided with display unit 7；It is provided above in the rear flank of frame 1 measuring analytic unit 8；Fluid path unit 9 it is provided with below the rear flank of frame 1；Below frame 1 rear flank and positive back upper place is provided with board control unit 10, board control unit 10 is connected with the circuit of the above remaining each unit, and is provided with central processing element (not shown) and is mounted with that systems soft ware is for controlling the automatic operation of remaining each unit action and complete machine.The spread configuration of above-mentioned each unit, makes full use of limited space so that analyser of the present invention is in the case of ensureing full-automatic function, and compact conformation, volume reduces the use that is easy to carry.
As shown in Fig. 1, Fig. 3, Fig. 7, Fig. 8, special agent unit 2 include dedicated kit 210, dedicated kit mounting groove 204, for stirring the mixing mechanism of special agent and for the first temperature control device for cooling 220 of low-temperature preservation dedicated kit 210, special agent unit 2 is arranged on the platform 11 of frame 1 by special agent unit mounting post 203.
As shown in Fig. 4-Fig. 8, dedicated kit 210 includes that box body 213, magnetic bead bottle 211, reagent bottle 212, magnetic bead bottle 211, reagent bottle 212 are the most vertically placed in box body 213, and magnetic bead bottle 211, reagent bottle 212 are respectively built with different special agents.Magnetic bead bottle 211 is freely rotatable in box body 213, magnetic bead bottle 211 includes the magnetic bead bottle main body 2110 of cylindrical shape, magnetic bead bottle main body 2110 bottom is provided with driving section 2111, driving section 2111 is exposed to box body 213, and driving section 2111 can be specifically gear, groove or the brake pad (not shown) that magnetic bead bottle surface is provided with.Mixing mechanism includes mixing motor 201, mixing driving member 202, mixing motor 201 drives the mixing driving member 202 coupled, mixing driving member 202 is connected with the driving section 2111 on magnetic bead bottle 211, therefore magnetic bead bottle 211 carries out reciprocating rotation therewith in mixing motor 201 can drive dedicated kit 210, carry out the mixing of special agent, it is to avoid special agent generation precipitation causes density unevenness.Mixing driving member 202 specifically can be made up of eccentric wheel assembly and transmission bar (not shown), for transmission bar is directed to driving section 2111, can be tooth bar, groove bar or fricting strip.
As shown in Fig. 7, Fig. 8, Fig. 9, the first temperature control device for cooling 220 is installed under some mounting grooves 204 of dedicated kit 210, carries out temperature control refrigeration；Dedicated kit mounting groove 204 be provided with can the base plate 221 of heat conduction (i.e. conducting refrigerating capacity), base plate 221 bottom is sequentially provided with refrigeration conductive plate 222, semiconductor refrigeration chip 223, fixed plate 224, fin 225 and fan 226；Fixed plate 224 for being fixed on the bottom of base plate 221 by refrigeration conductive plate 222, semiconductor refrigeration chip 223, fin 225 and fan 226；Semiconductor refrigeration chip 223 includes that cold and hot two sides, hot side contact with fin 225, and huyashi-chuuka (cold chinese-style noodles) connects refrigeration conductive plate 222, refrigeration conductive plate 222 and base plate 221 lower contacts, and the top of base plate 221 is dedicated kit mounting groove 204.When first temperature control device for cooling 220 works, semiconductor refrigeration chip 223 huyashi-chuuka (cold chinese-style noodles) produces cold, cold conducts to dedicated kit mounting groove 204 through semiconductor refrigeration chip 223, refrigeration conductive plate 222, base plate 221, makes dedicated kit mounting groove 204, dedicated kit 210 and interior special agent thereof produce cold storage effect and be automatically adjusted stable at a temperature of certain；Semiconductor refrigeration chip 223 hot side produces heat simultaneously, and heat conducts to air through fin 225 and fan 226.
As shown in Figure 1, Figure 10 shows, sample storehouse unit 3 is arranged on the platform 11 of frame 1 by sample storehouse unit mounting post 302, sample storehouse unit 3 for carrying out the input of sample with user interface, and sample storehouse unit 3 includes sample rack 310, sample rack mounting seat 301, sample rack positioner 303 and service sink assembly 304.In the present embodiment, sample rack mounting seat 301 is an installing plate with guide groove 305, and sample rack positioner 303 is a location resilient plunger.Sample is loaded in sample tube (not shown), sample tube is positioned in sample rack 310, sample rack 310 inserts in the guide groove 305 of sample rack mounting seat 301, positioning elastic plunger 303 is arranged in guide groove 305, it is used for guaranteeing that sample rack 310 inserts guide groove 305 to specifying position, and there is the effect of fixed position, and make sample rack 310 avoid being moved after putting in place, beneficially sample needle accurately stretches into sample tube and is sampled.
As shown in figure 11, for the schematic construction of sample rack 310, sample rack 310 has the Handheld Division 31A that can manually grasp and places the slotted eye 31B of sample tube.
As shown in Figure 10, sample service sink assembly 304 is used for cleaning sample needle 605(as shown in figure 18) sampling after cleaning, it is installed on sample rack installing plate 301 rear portion, sample service sink assembly 304 includes service sink (not shown), detergent line (not shown), it is provided with waste liquid outlet (not shown) bottom sample service sink assembly 304 and is connected to fluid path unit 9, clean the waste liquid produced and entered the waste liquid tank (not shown) of fluid path unit 9 by pipeline.Service sink top is provided with rinse water outlet (not shown), and rinse water is flowed out by outlet, can be carried out sample needle 605 inside and outside wall.
As shown in Fig. 1, Figure 12-Figure 14, hatching unit 4 and set support 400, support 400 is arranged on the platform 11 of frame 1 by hatching unit mounting post 407.Support 400 is provided with rotatable hatches dish 405, support 400 is arranged below and hatches motor 401, Timing Belt 401A hatches dish 405 in order to drive, dish is put have integrate multiple hatch cup 432 hatch cup group 403, hatch dish 405 bottom and be provided with temperature control heating diaphragm 406, temperature control heating diaphragm 406 can be to hatching dish 405 and interior cup group 403 of hatching heats, and temperature is controlled at about 38 DEG C, simulation human body temperature environment, makes the incubation reaction liquid hatching in cup 432 carry out good incubation reaction.Hatch dish 405 to can be rotated to different cup 432 of hatching and can coordinate from sample needle 605 and add different samples and reagent reacts simultaneously.
As shown in Figure 12 and Figure 13, hatch dish 405 and be provided with hatch disk cover 409 in box-like, the top of circle, hatch disk cover 409 can prevent foreign material fall into hatch dish 405 in or fall into and hatch in cup 432, also there is certain heat insulation effect simultaneously.Hatch disk cover 409 to be provided with and hatch cup group gateway 4091 and be provided with adding mouth 4092, be provided with and hatch cup 432 in hatching dish 405 and can hatch hole 4051 with what one_to_one corresponding can accommodating hatched cup 432.Hatching cup group gateway 4091 and hatch a glass group in order to convenient loading or taking-up, adding mouth 4092 is in order to facilitate sample needle 605 or post processing pin 608(as shown in figure 18) pass so that it is can be plugged into hatching in cup 432 to extract or injecting sample, reagent or reactant liquor.
nullSuch as Figure 12、Figure 15、Shown in Figure 16，Four groups can be put in hatching dish 405 and hatch cup group 403，Hatch cup group 403 and include that some identical on glass stand 431 of the ring-type glass stand 431 of quadrant and vertical sleeve hatches cup 432，Hatch cup 432 and be provided with the cup inner chamber 4320 that can contain reactant liquor，Hatch cup 432 and include cup 4321 and lower cup body 4322，Upper cup 4321 is in a tubular form，The inside and outside wall of lower cup body 4322 receive cup 4321 after in the inverted conical shape being gradually reduced and bottom transition is circular shape，Glass stand 431 is provided with glass holder hole 4310，Upper cup 4321 unremovably sleeve is in glass holder hole 4310，Make to hatch cup 432 to fix as one with glass stand 431，Lower cup body 4322 is exposed to glass stand 431 can to make to hatch cup 432 and being inserted into and hatch one to one in hole 4051.Hatch cup group 403 and integrate multiple cup 432 of hatching, and hatch cup 432 and fix as one with glass stand 431, can conveniently hatch cup 432 and hatch packed and transported and the transportation safety of cup group 403, another change quickness and high efficiency when hatching cup 432.
As shown in figures 12-16, in the present embodiment, hatch unit 4 and hatch work for carrying out heating of sample, sample mixed with reagent adds hatches in cup 432, hatching cup group 403 to be positioned over and hatch dish 405 and hatch in hole 4051 accordingly, each cup 432 correspondence of hatching is hatched one of dish 405 and is hatched hole 4051.Hatch motor 401 to drive and hatch dish 405 and rotate so that the corresponding actions of cooperative mechanical arm unit 6.Temperature control heating diaphragm 406 is installed on to hatch and carries out temperature control heating to hatching dish 405 below dish 405.Hatch dish and be maintained at about 38 DEG C work, need to simulate human body environment because hatching dish, to guarantee antigen and effective combination of antibody in immunoreation.
As shown in figure 14, hatch dish 405 to be provided with further below to follow and hatch the code-disc 408 that dish 405 rotates together, code-disc 408 is a rotating disk being provided with yard tooth, code-disc 408 bottom be provided with detection code-disc original position code-disc zero-bit induction apparatus 411 and can the code-disc rotation precision induction apparatus 412 of sensing code tooth change.
As shown in Figure 12-Figure 14, in the present embodiment, hatch unit 4 and use code-disc 408, rotation precision induction apparatus 412 detects code-disc code tooth rotational angle and carries out the mode of signal feedback, control to hatch the rotation precision of dish 405 to be accurately loaded, its principle is that each hatches a teeth portion gap of the corresponding code-disc 408 in hole 4051, if there is error or the reaction gap error that trace step-out causes in rotating in certain, will not be this error accumulation to rotating next time, rotate relatively independent i.e. every time, thereby ensure that hatch the precision that dish 405 rotates, ensure that the sample needle 605 of mechanical arm unit 6 and post processing pin 608(are as shown in figure 18) can accurately be inserted into and hatch each of cup group 403 and hatch in cup 432.
As shown in Fig. 1, Figure 12, in the present embodiment, hatch unit 4 to be additionally provided with and hatch cleaning assembly 402 and wash bowl 404, hatch service sink assembly 402(identical with sample service sink assembly 304 structure) it is installed on and hatches on the right side of dish 405, it is connected with fluid path unit by detergent line.Wash bowl 404 is installed on hatches dish 405 front.Hatch service sink assembly 402 be post processing pin 608(as shown in figure 18) the conventional place cleaned, hatch service sink assembly 402 and by adding rinse water, post processing pin 608 inside and outside wall can be carried out conventional cleaning, clean the waste liquid produced and enter the waste liquid tank (not shown) of fluid path unit 9 through pipeline.Wash bowl 404 is for storage system strong cleanout fluid reagent bottle (not shown), and system is finishing high level sample or use must clean project by force when, it should use strong cleanout fluid to sample needle 605(as shown in figure 18) it is carried out.When strong cleanout fluid uses, sample needle 605 draw and be stored in sample needle 605 internal, clean the waste liquid produced and drained by sample service sink assembly 304.
Such as Fig. 1, shown in Figure 17, common reagent unit 5 is arranged on the platform 11 of frame 1 by common reagent unit mounting post 504, common reagent unit 5 includes universal kit 501, the second temperature control device for cooling 503 that universal kit mounting groove 502 and its underpart are provided with, common reagent unit 5 is used for storing common reagent and temperature control effect, universal kit 501 is placed in universal kit mounting groove 502, second temperature control device for cooling 503(structure is identical with the first temperature control device for cooling 220) it is installed on below universal kit mounting groove 502 in order to common reagent is carried out temperature control storage.
As shown in Fig. 1, Figure 18, the back upper place of platform 11 is provided with upper mounting plate 12, upper mounting plate 12 is provided with mechanical arm unit 6, mechanical arm unit 6 includes the first mechanical arm 61 of band sample needle 605 and the second mechanical arm 62 of band post processing pin 608, under the drive respectively of first mechanical arm the 61, second mechanical arm 62, sample needle 605 can at special agent unit 2, sample storehouse unit 3, hatch and move freely above unit 4, post processing pin 608 can move freely above unit 4, common reagent unit 5 hatching.Mechanical arm unit 6 for taking liquid, moving liquid effect to each sample and reagent, incubation reaction liquid etc..
As shown in figure 18, in the present embodiment, the first mechanical arm 61 with sample needle 605 is three-dimensional arm, the second mechanical arm 62 with post processing pin 608 is two dimension arm, three-dimensional arm 61 and two dimension arm 62 are arranged on the two ends of the same X-direction guide rail 601 that upper mounting plate 12 is provided with, three-dimensional arm 61 be included in can move freely on X-direction guide rail 601 three-dimensional Y-axis arm 602, be arranged on three-dimensional Y-axis arm 602 the three-dimensional Z axis arm 603 that can move freely along Y-direction, sample needle 605 is parallelly mounted on three-dimensional Z axis arm 603 and can move freely along Z-direction；The two-dimentional Z axis arm 607 that two dimension arm 62 is included in the two-dimentional Y-axis arm 606 that can move freely on X-direction guide rail 601, is fixedly mounted on two dimension Y-axis arm 606, post processing pin 608 is parallelly mounted on two dimension Z axis arm 607 and can move freely along Z-direction.Three-dimensional arm 61 and the two dimension arm 62 motion on each movable direction are driven by respective motor and Timing Belt respectively, and such as three-dimensional arm 61 in the X direction, is provided with motor 610 and drives Timing Belt 611 to drive three-dimensional Y-axis arm 602 to move left and right on X-direction guide rail 601.
As shown in Fig. 1, Figure 19, display unit 7 is connected with described board control unit 10 circuit, for carrying out human-computer interaction with user, display unit 7 includes tangibly display screen 703, for installing the display screen outer housing 704 of display screen 703, display screen rotating shaft 702, display screen support bar 705 and scope rack 701.Display screen 703 is installed in display screen outer housing 704, and display screen 703 can be rotated by display screen outer housing 704 by display screen rotating shaft 702, is adjusted to adapt to the possible angle that user uses and watches.Display screen support bar 705 can be fixed after display screen 703 turns to possible angle.Display unit 7 is installed in frame 1 entirely through scope rack 701.
nullSuch as Fig. 1、Fig. 2、Shown in Figure 20，The measurement analytic unit 8 being connected with common reagent unit 5 fluid path it is additionally provided with in frame 1，Measure analytic unit 8 and include the measurement assembly 80 of the sample for measuring incubation reaction、Outer package assembly 81、For controlling to measure the temperature-controlling component 82 of assembly temperature、And analyzing and processing circuit (not shown)，Temperature-controlling component 82 is arranged at the outer surface of outer package assembly 81，Measure assembly 80 lucifuge be arranged at outer package assembly 81 inside and contact with temperature-controlling component 82 inner surface，Measure assembly 80 and include measuring cell 801，The photomultiplier tube (PMT) 802 being arranged over for reading fluorescence of measuring cell 801，The outer bottom of measuring cell 801 is provided with separable suction pearl Magnet 803 of can fitting therewith，Measuring cell 801 connects feed tube 804 and drain pipe 805，Feed tube 804 and drain pipe 805 lucifuge stretch out in outer package assembly 81.
As shown in figure 22, in the present embodiment, measuring assembly 80 is main framework by dilatory substrate 800, the measuring cell 801 of liquid process is arranged on below dilatory substrate 800, accurately being installed on directly over measuring cell 801 for reading the photomultiplier tube (PMT) 802 of fluorescence, photomultiplier tube (PMT) 802 is externally provided with sleeve pipe 808 in order to lucifuge.The lower surface measuring assembly 80 is provided with swing-bar mechanism, oscillating motor 806 drive fork 807, thus fit to or separate measuring cell 801 outer bottom by the suction pearl Magnet 803 that fork 807 end is arranged.When incubation reaction sample by feed tube 804 enter measurement analytic unit 8 arrive measuring cell 801 time, suction pearl Magnet 803 is fitted to measuring cell 801 outer bottom by oscillating motor 806 fork 807, and in sample, magnetic bead with label is inhaled pearl Magnet 803 and held.Measuring cell 801 is internal is additionally provided with positive and negative electrode, and when electrifying electrodes, in sample, the magnetic bead with label can send week fluorescent, now photomultiplier tube (PMT) 802 work, the week fluorescent that in reading a period of time, magnetic bead sends.
As shown in Fig. 2, Figure 23, fluid path unit 9 is used for controlling sample and flows to reagent and waste liquid, and fluid path unit 9 is installed on support 901, and support 901 is installed in frame 1 to ensure overall property easy to maintenance.Syringe 902 that fluid path unit 9 includes being connected with sampling needle 605, post processing pin 608 respectively, hydraulic pump 903, hydraulic valve 904, and it is connected to discharge the negative pressure pump (not shown) needed for waste liquid, for collecting the waste liquid tank (not shown) of waste liquid with measuring unit 8.
The above, special agent refers to the reagent of immunoreation project, in usual each project, the solution volume of dedicated kit is not quite similar, the reagent reacting principle of concrete consumption and this project of serving as reference, when special agent uses, drawn by sample needle 605, and be respectively added to hatch cup with sample.Common reagent refers to that all items measurement, instrument initialization etc. need the system reagent used.Common reagent belongs to measures the reagent that analytic unit 8 is exclusive, post processing pin 608 draw.
As shown in Fig. 1-Figure 22, three-dimensional arm 61 can drive sample needle 605 to be filled into after being drawn respectively by the sample in the special agent in special agent unit 2 and sample storehouse unit 3 respectively and hatch hatching in unit 4 and carry out incubation reaction in cup 432, and two dimension arm 62 can drive post processing pin 608 that by pipeline, the common reagent in the sample of the incubation reaction hatched in cup 432 and common reagent unit 5 is drawn onto reaction in the measuring cell 801 measured in analytic unit 8.
As shown in figure 24, the survey analysis method of small electrical chemical illumination immunity analysis instrument of the present invention, including following measurement analytical procedure:
(1) preparation is measured: start analyser, by system initialization；Load measuring required special agent to the dedicated kit 210 of special agent warehouse receipt unit 2, and carry out magnetic bead stirring (avoiding precipitating) by mixing mechanism；Common reagent needed for measuring loads to the universal kit 501 of common reagent unit 5；Put into hatch the hatching in dish 405 of unit 4 by hatching cup group 403；The test tube that will be equipped with sample loads in sample rack 310, and then sample rack 310 pushes sample storehouse unit 3, input catalogue number(Cat.No.) bar code；
(2) measurement is started: starting at display unit 7 and measure button, analyser enters following all automatic measurement program；
(3) incubation step one: sample needle 605 extracts required special agent one from dedicated kit 210 automatically by measure the item, then pass through the sample service sink assembly 304 being provided with in sample storehouse unit 3 sample needle 605 is carried out, sample in rear absorption sample tube, being subsequently adding to hatch in cup group 403 available hatches in cup 432, hatching the dish 405 liquid to hatching in cup 432 and carry out 38 DEG C of insulation incubation reaction, incubation step one is reaction timing by the systems soft ware installed in board control unit 10；
(4) incubation step two: after incubation step one terminates, the required special agent two of extraction in the automatic dedicated kit 210 of sample needle 605, add completed incubation step one hatch in cup 432, and carry out compressing mixing, hatching the dish 405 liquid to hatching in cup 432 and carry out 38 DEG C of insulation incubation reaction, incubation step two is reaction timing by the systems soft ware installed in board control unit 10；
(5) Beads enrichment: measure analytic unit 8 and be automatically performed measuring cell 801 preparation before incubation step two completes；After incubation step two completes, post processing pin 608 automatically extends into hatch draws the sample completing incubation reaction in cup 432, and the sample having completed incubation reaction flows in measuring cell 801 along the pipeline being connected with post processing pin 608；The oscillating motor 806 measuring analytic unit 8 drives fork 807, makes the suction pearl Magnet 803 being arranged on fork 807 fit to measuring cell 801 outer bottom, has completed magnetic bead in the sample of incubation reaction by magneticaction, and absorption is bottom measuring cell 801；Post processing pin 608 extracts common reagent one (BUFFER) automatically to measuring cell 801, and rinses magnetic bead；Complete after magnetic bead cleans, to keep liquid in runner static；Pearl Magnet 803 clear point is inhaled in release；
(6) analysis is measured: measuring cell 801 electrifying electrodes, shiner contained on magnetic bead produces fluorescence, photomultiplier tube 802 is converted into the signal of telecommunication after receiving fluorescence, measure analytic unit 8 to be analyzed this signal of telecommunication processing, the systems soft ware uploading to after obtaining signal value install in board control unit 10 processes, and result is shown in display unit 7 automatically；
(7) electrode reduction: post processing pin 608 automatically extracts common reagent two (AFFER) and cleans measuring cell 801 and pipeline thereof, and measuring cell 801 electrode reversely powers up, and makes electrode reduce；
(8) measuring unit resets: the waste liquid that measuring cell 801 is measured generation is discharged in the waste liquid tank of fluid path unit 9, and post processing pin 608 again extracts common reagent one (BUFFER) and fills measuring unit 8 associated pipe and prepare next round measurement；
(9) measurement terminates: measuring and terminate, analyser is in holding state.
Described above is only presently preferred embodiments of the present invention, and above-mentioned specific embodiment is not limitation of the present invention.In the technological thought category of the present invention, various deformation and amendment can occur, retouching, amendment or the equivalent that all those of ordinary skill in the art are made as described above, belong to the scope that the present invention is protected.
Claims (15)
1. a small electrical chemical illumination immunity analysis instrument, including bottom be provided with the frame of platform, it is characterised in that: described platform is provided with special agent unit, sample storehouse unit, hatches unit, common reagent unit；Described special agent unit include dedicated kit, dedicated kit mounting groove, for stirring the mixing mechanism of special agent and for the first temperature control device for cooling of low-temperature preservation dedicated kit；Described hatch unit include by hatch motor drive rotate hatch dish, hatch dish is put integrate multiple and hatch hatching glass group and hatching the temperature control heating diaphragm that dish bottom is provided with of cup；Described common reagent unit includes the second temperature control device for cooling that universal kit, universal kit mounting groove and its underpart are provided with；Described frame back upper place is provided with upper mounting plate, described upper mounting plate is provided with mechanical arm unit, described mechanical arm unit includes being provided with the first mechanical arm of sample needle and being provided with the second mechanical arm of post processing pin, described sample needle under described first mechanical arm drives can at described special agent unit, sample storehouse unit, hatch and move freely above unit, described post processing pin can move freely above unit, common reagent unit described hatching under described second mechanical arm drives；It is additionally provided with in described frame and measures analytic unit, for controlling sample and the reagent flow direction and collecting the fluid path unit of waste liquid, for carrying out the display unit of man-machine interaction and being connected to central controlled board control unit with remaining each unit circuit described.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterized in that: described first temperature control device for cooling or the second temperature control device for cooling, including can the base plate of heat conduction, described base plate is provided with refrigeration conductive plate in turn below, semiconductor refrigeration chip, fin and fan, described semiconductor refrigeration chip includes cold and hot two sides, described hot side is fitted and connected with described fin one side, described fan is connected to dispel the heat with described fin another side, described huyashi-chuuka (cold chinese-style noodles) is fitted and connected with described refrigeration conductive plate one side, described refrigeration conductive plate another side is fitted and connected with described base plate lower plane, on described base plate, plane is provided with described dedicated kit mounting groove or universal kit mounting groove.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterized in that: described dedicated kit includes the magnetic bead bottle of storing, reagent bottle in box body, box body, special agent can be filled respectively in described magnetic bead bottle, reagent bottle, described magnetic bead bottle is freely rotatable, and its underpart is provided with driving section and is exposed to described box body；Described mixing mechanism includes mixing motor, mixing driving member, and described mixing driving member is connected with the driving section of described magnetic bead bottle, and described mixing motor can drive described mixing driving member to drive described magnetic bead bottle to carry out reciprocating rotation.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterized in that: described sample storehouse unit includes that described sample service sink assembly includes sample service sink, the detergent line being connected with described fluid path unit, waste liquid discharge line for putting the sample rack of sample tube, sample rack mounting seat, sample rack positioner and sample service sink assembly.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterized in that: described in hatch dish in circle box-like, internal be provided with described hatch glass one_to_one corresponding and hatch cup described in can accommodating hatch hole, described hatch dish in can put and described in four groups, hatch a glass group, described in hatch glass group and include that some on glass stand of the ring-type glass stand of quadrant and vertical sleeve hatch cup.
Small electrical chemical illumination immunity analysis instrument the most according to claim 5, it is characterized in that: described in hatch cup and be provided with the cup inner chamber that can contain reactant liquor, described cup of hatching includes cup and lower cup body, described upper cup is in a tubular form, the inside and outside wall of described lower cup body receive described upper cup after in the inverted conical shape being gradually reduced and bottom transition is circular shape, described glass stand is provided with glass holder hole, described upper cup unremovably sleeve is in described glass holder hole, and described lower cup body is exposed to described glass stand.
Small electrical chemical illumination immunity analysis instrument the most according to claim 5, it is characterised in that hatch dish top described in: and be provided with and hatch disk cover, described in hatch disk cover and be provided with adding mouth, hatch the gateway of cup group for loading and take out.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterised in that hatching unit described in: and also include hatching, with described, the code-disc that dish is coaxially disposed, described code-disc bottom is provided with code-disc zero-bit induction apparatus and code-disc rotation precision induction apparatus.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterized in that: described in hatch unit and also include hatching cleaning assembly and wash bowl, described cleaning assembly of hatching includes hatching service sink, the detergent line being connected with described fluid path unit, waste liquid discharge line, and described wash bowl is used for storage system strong cleanout fluid reagent bottle.
Small electrical chemical illumination immunity analysis instrument the most according to claim 1, it is characterized in that: described first mechanical arm is three-dimensional arm, described second mechanical arm is two dimension arm, described three-dimensional arm and two dimension arm are arranged on the same X-direction guide rail that described upper mounting plate is provided with, the three-dimensional Z axis arm that can move freely along Y-direction that described three-dimensional arm is included in the three-dimensional Y-axis arm that can move freely on X-direction guide rail, installs on described three-dimensional Y-axis arm, described sample needle is parallelly mounted on described three-dimensional Z axis arm and can move freely along Z-direction；Described two dimension arm be included in can move freely on X-direction guide rail two-dimentional Y-axis arm, described two dimension Y-axis arm on hard-wired two dimension Z axis arm, described post processing pin be parallelly mounted to described two dimension Z axis arm on and can move freely along Z-direction；Described three-dimensional arm and two dimension arm motion on each movable direction are driven by respective motor and Timing Belt respectively.
11. small electrical chemical illumination immunity analysis instruments according to claim 1, it is characterized in that: described measurement analytic unit includes for measuring the measurement assembly having hatched sample, outer package assembly, for controlling the temperature-controlling component of described measurement assembly temperature, analyzing and processing circuit, described temperature-controlling component is arranged at described outer package module outer surface, described measurement assembly lucifuge is arranged at described outer package component internal and contacts with described temperature-controlling component inner surface, described measurement assembly includes measuring cell, the photomultiplier tube being arranged over for reading fluorescence of described measuring cell, outer bottom is provided with separable suction pearl Magnet of can fitting therewith, inside is provided with positive and negative electrode, described measuring cell is also associated with feed tube and drain pipe, described feed tube and drain pipe lucifuge stretch out in described outer package assembly.
12. small electrical chemical illumination immunity analysis instruments according to claim 1, it is characterized in that: syringe that described fluid path unit includes being connected with described sampling needle, post processing pin respectively, hydraulic pump, hydraulic valve, liquid line, and be connected to discharge the negative pressure pump needed for waste liquid, for collecting the waste liquid tank of waste liquid with measuring unit.
13. small electrical chemical illumination immunity analysis instruments according to claim 1, it is characterized in that: described display unit is connected with described board control unit circuit, including tangibly display screen, for installing the display screen outer housing of described display screen, display screen rotating shaft, display screen support bar and scope rack.
14. small electrical chemical illumination immunity analysis instruments according to claim 1, it is characterized in that: described board control unit is provided with central processing element and is provided with systems soft ware, described board control unit can concentrate the action coordinating to control remaining each unit, and can carry out processing and result being sent to display unit by the signal that described measurement analytic unit records.
The survey analysis method of 15. 1 kinds of small electrical chemical illumination immunity analysis instruments according to claim 1, it is characterised in that include following measurement analytical procedure:
(1) preparation is measured: start analyser, by system initialization；Load measuring required special agent to the dedicated kit of special agent warehouse receipt unit, and carry out magnetic bead stirring by mixing mechanism；Common reagent needed for measuring loads to the universal kit of common reagent unit；Put into hatch the hatching in dish of unit by hatching glass group；Will be equipped with, in the sample rack of test tube loading sample storehouse unit of sample, then sample rack pushing sample storehouse unit, input catalogue number(Cat.No.) bar code；
(2) measurement is started: starting at display unit and measure button, analyser enters following all automatic measurement program；
(3) incubation step one: sample needle presses measure the item automatically from the required special agent one of dedicated kit extraction, then pass through the sample service sink assembly being provided with in sample storehouse unit sample needle is cleaned, sample in the sample tube put in rear absorption sample storehouse unit, being subsequently adding to hatch in cup group available hatches in cup, hatching the dish liquid to hatching in cup and carry out 38 DEG C of insulation incubation reaction, incubation step one is reaction timing by the systems soft ware installed in board control unit；
(4) incubation step two: after incubation step one terminates, the sample needle required special agent two of extraction from dedicated kit automatically, add completed incubation step one hatch in cup, and carry out compressing mixing, hatching the dish liquid to hatching in cup and carry out 38 DEG C of insulation incubation reaction, incubation step two is reaction timing by the systems soft ware installed in board control unit；
(5) Beads enrichment: measure analytic unit and be automatically performed measuring cell preparation before incubation step two completes；After incubation step two completes, post processing pin automatically extends into hatch draws the sample completing incubation reaction in cup, and the sample having completed incubation reaction flows in measuring cell along the pipeline being connected with post processing pin；Measuring the oscillating motor that is provided with of analytic unit and drive the fork being attached thereto, make the suction pearl Magnet arranged on fork fit to measuring cell outer bottom, complete the magnetic bead in the sample hatched by magneticaction, absorption is bottom measuring cell；Then post processing pin extracts common reagent one to measuring cell automatically, and rinses magnetic bead；Complete after magnetic bead cleans, to keep liquid in runner static；Pearl Magnet clear point is inhaled in oscillating motor release；
(6) analysis is measured: in measuring cell, be provided with positive and negative electrode, positive and negative electrode is energized, shiner contained on magnetic bead in measuring cell produces fluorescence because of energising, photomultiplier tube is converted into the signal of telecommunication after receiving fluorescence, measure analytic unit to be analyzed this signal of telecommunication processing, the systems soft ware uploading to after obtaining signal value install in board control unit processes, and result is shown in display unit automatically；
(7) electrode reduction: post processing pin automatically extracts common reagent two and cleans measuring cell and pipeline thereof, reversely powers up positive and negative electrode in measuring cell, makes electrode reduce；
(8) measuring unit resets: the waste liquid produced after being measured by measuring cell is discharged in the waste liquid tank that fluid path unit is provided with, and post processing pin again extracts common reagent one and fills measuring unit associated pipe and prepare next round measurement；
(9) measurement terminates: measuring and terminate, analyser is in holding state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610521811.5A CN106018784B (en) | 2016-07-05 | 2016-07-05 | Small electrical chemical illumination immunity analysis instrument and its analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610521811.5A CN106018784B (en) | 2016-07-05 | 2016-07-05 | Small electrical chemical illumination immunity analysis instrument and its analysis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106018784A true CN106018784A (en) | 2016-10-12 |
| CN106018784B CN106018784B (en) | 2019-02-22 |
Family
ID=57106548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610521811.5A Active CN106018784B (en) | 2016-07-05 | 2016-07-05 | Small electrical chemical illumination immunity analysis instrument and its analysis method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106018784B (en) |
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834114A (en) * | 2017-02-20 | 2017-06-13 | 佛山市金蓝领教育科技有限公司 | A kind of automated detection system of PCR-based application |
| CN107045067A (en) * | 2017-03-27 | 2017-08-15 | 武汉优视科技有限公司 | A kind of liquid-adding device for Western blotting |
| CN107132345A (en) * | 2017-06-22 | 2017-09-05 | 深圳迈奇生物科技有限公司 | A kind of biological chip reading apparatus |
| CN107557356A (en) * | 2017-09-05 | 2018-01-09 | 苏州普川生物科技有限公司 | A kind of small multi-channel library construction instrument and the method for building library |
| CN108120840A (en) * | 2018-01-19 | 2018-06-05 | 深圳市亚辉龙生物科技股份有限公司 | Full-automatic immunoblotting assay instrument |
| CN108169501A (en) * | 2017-12-04 | 2018-06-15 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
| CN108614101A (en) * | 2018-03-14 | 2018-10-02 | 嘉兴科瑞迪医疗器械有限公司 | A kind of magnetic particle Full-automatic chemiluminescence immunoassay analysis meter |
| CN109061208A (en) * | 2018-08-02 | 2018-12-21 | 上海宝中盈仪器仪表有限公司 | Full-automatic biochemical oxygen demand detector |
| CN109324200A (en) * | 2018-12-12 | 2019-02-12 | 深圳普门科技股份有限公司 | Sample analyser |
| CN109342744A (en) * | 2018-12-12 | 2019-02-15 | 深圳普门科技股份有限公司 | A kind of the space layout method and its test method of specific protein analyzer |
| CN109507438A (en) * | 2018-11-08 | 2019-03-22 | 迪瑞医疗科技股份有限公司 | A kind of linear incubation slot and its automatic clinical chemistry analyzer |
| CN109580932A (en) * | 2019-02-26 | 2019-04-05 | 烟台艾德康生物科技有限公司 | A kind of Full-automatic magnetic beads entry joint inspection detection analysis instrument and its application method |
| CN110040889A (en) * | 2019-05-06 | 2019-07-23 | 安徽科技学院 | A kind of sewage-treatment plant based on heavy metal pollution |
| CN110133310A (en) * | 2019-05-08 | 2019-08-16 | 东软威特曼生物科技（沈阳）有限公司 | Biochemical Analyzer |
| CN110320353A (en) * | 2019-07-20 | 2019-10-11 | 中国人民解放军总医院 | A kind of fluorescence immunity analyzer and sample testing method applied to altitude environment |
| CN110456089A (en) * | 2019-08-28 | 2019-11-15 | 迈克医疗电子有限公司 | Full-automatic sample analyser and its additional examination agent method, computing device |
| WO2019222445A1 (en) * | 2018-05-18 | 2019-11-21 | Meso Scale Technologies, Llc. | High throughput system for performing assays using electrochemiluminesence including a consumable shaking apparatus |
| CN110967495A (en) * | 2018-09-28 | 2020-04-07 | 深圳市帝迈生物技术有限公司 | Analyzer |
| CN111366436A (en) * | 2020-04-30 | 2020-07-03 | 中国人民解放军总医院 | Full-automatic semen processing device |
| CN112578134A (en) * | 2019-09-30 | 2021-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analysis system and control method thereof |
| CN113567690A (en) * | 2020-04-28 | 2021-10-29 | 深圳迎凯生物科技有限公司 | Incubation assembly, incubation device and automatic analysis device |
| WO2021217441A1 (en) * | 2020-04-28 | 2021-11-04 | 深圳迎凯生物科技有限公司 | Incubation assembly, incubation device and automatic analysis device |
| CN114675043A (en) * | 2017-03-28 | 2022-06-28 | 科美诊断技术股份有限公司 | Control method of immunoassay device |
| CN114814261A (en) * | 2022-04-21 | 2022-07-29 | 华中科技大学 | Automatic chemiluminescence immunoassay chip and detection method thereof |
| CN116660569A (en) * | 2023-07-17 | 2023-08-29 | 中国科学技术大学 | Multifunctional quantitative liquid dripping workstation |
| CN117233369A (en) * | 2023-11-15 | 2023-12-15 | 长沙迈迪克智能科技有限公司 | Incubation assembly for medical instrument and medical instrument |
Citations (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4774055A (en) * | 1985-06-26 | 1988-09-27 | Japan Tectron Instruments Corporation | Automatic analysis apparatus |
| US5051238A (en) * | 1987-11-20 | 1991-09-24 | Hitachi, Ltd. | Automatic analyzing system |
| JP2001027643A (en) * | 1999-05-11 | 2001-01-30 | Sysmex Corp | Automatic analyzer |
| US20070104614A1 (en) * | 2005-11-10 | 2007-05-10 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Automatic chemistry analyzer and analyzing method |
| CN201689093U (en) * | 2010-01-04 | 2010-12-29 | 深圳市亚辉龙生物科技有限公司 | Full-automatic infection immunity analyzer |
| CN201765226U (en) * | 2010-04-06 | 2011-03-16 | 合肥运涛光电科技有限公司 | Dial type direct-reading fully-automatic immune luminescence analysis system |
| CN102147406A (en) * | 2011-02-14 | 2011-08-10 | 四川迈克生物科技股份有限公司 | Fully automatic chemical luminescent immune analyzer |
| CN202075285U (en) * | 2011-04-02 | 2011-12-14 | 合肥运涛光电科技有限公司 | Minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding sample in whole disc |
| CN102445557A (en) * | 2011-09-27 | 2012-05-09 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immunity analyzer and detection method thereof |
| CN102565439A (en) * | 2011-12-29 | 2012-07-11 | 长春迪瑞医疗科技股份有限公司 | Incubation part device for full-automatic chemiluminescence immunity analyzer and control method for incubation part device |
| CN102998473A (en) * | 2012-12-19 | 2013-03-27 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer |
| CN103399161A (en) * | 2013-07-17 | 2013-11-20 | 深圳市新产业生物医学工程股份有限公司 | Portable full-automatic chemiluminescence immune assay system and assay method thereof |
| CN103499701A (en) * | 2013-09-04 | 2014-01-08 | 深圳市锦瑞电子有限公司 | Specific protein analyzer and analysis method thereof |
| CN203561641U (en) * | 2013-11-28 | 2014-04-23 | 博奥赛斯（天津）生物科技有限公司 | Chemical luminescence immunity analyzer structure |
| CN104297498A (en) * | 2013-07-16 | 2015-01-21 | 合肥运涛光电科技有限公司 | Fully automatic biochemical and luminescent immunoassay system |
| CN104714042A (en) * | 2013-12-16 | 2015-06-17 | 深圳市亚辉龙生物科技有限公司 | Full-automatic chemiluminescence immune analyzer and use method thereof |
| CN104777321A (en) * | 2015-04-16 | 2015-07-15 | 清华大学 | Chemiluminescence immunoassay device |
| CN105628687A (en) * | 2016-02-24 | 2016-06-01 | 南京诺尔曼生物技术有限公司 | Full-automatic chemiluminiscence tester |
| CN105628947A (en) * | 2016-02-02 | 2016-06-01 | 徐恩良 | Whole blood autoluminescence immunoassay point-of-care test instrument |
| CN105628948A (en) * | 2016-03-04 | 2016-06-01 | 深圳普门科技有限公司 | High-speed C reactive protein analyzer and analyzing method thereof |
| CN206339561U (en) * | 2016-07-05 | 2017-07-18 | 深圳普门科技有限公司 | Small electrical chemical illumination immunity analysis instrument |
-
2016
- 2016-07-05 CN CN201610521811.5A patent/CN106018784B/en active Active
Patent Citations (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4774055A (en) * | 1985-06-26 | 1988-09-27 | Japan Tectron Instruments Corporation | Automatic analysis apparatus |
| US5051238A (en) * | 1987-11-20 | 1991-09-24 | Hitachi, Ltd. | Automatic analyzing system |
| JP2001027643A (en) * | 1999-05-11 | 2001-01-30 | Sysmex Corp | Automatic analyzer |
| US20070104614A1 (en) * | 2005-11-10 | 2007-05-10 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Automatic chemistry analyzer and analyzing method |
| CN1963527A (en) * | 2005-11-10 | 2007-05-16 | 深圳迈瑞生物医疗电子股份有限公司 | Full-automatic biochemical analyzer and analysis method thereof |
| CN201689093U (en) * | 2010-01-04 | 2010-12-29 | 深圳市亚辉龙生物科技有限公司 | Full-automatic infection immunity analyzer |
| CN201765226U (en) * | 2010-04-06 | 2011-03-16 | 合肥运涛光电科技有限公司 | Dial type direct-reading fully-automatic immune luminescence analysis system |
| CN102147406A (en) * | 2011-02-14 | 2011-08-10 | 四川迈克生物科技股份有限公司 | Fully automatic chemical luminescent immune analyzer |
| CN202075285U (en) * | 2011-04-02 | 2011-12-14 | 合肥运涛光电科技有限公司 | Minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding sample in whole disc |
| CN102445557A (en) * | 2011-09-27 | 2012-05-09 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immunity analyzer and detection method thereof |
| CN102565439A (en) * | 2011-12-29 | 2012-07-11 | 长春迪瑞医疗科技股份有限公司 | Incubation part device for full-automatic chemiluminescence immunity analyzer and control method for incubation part device |
| CN102998473A (en) * | 2012-12-19 | 2013-03-27 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer |
| CN104297498A (en) * | 2013-07-16 | 2015-01-21 | 合肥运涛光电科技有限公司 | Fully automatic biochemical and luminescent immunoassay system |
| CN103399161A (en) * | 2013-07-17 | 2013-11-20 | 深圳市新产业生物医学工程股份有限公司 | Portable full-automatic chemiluminescence immune assay system and assay method thereof |
| CN103499701A (en) * | 2013-09-04 | 2014-01-08 | 深圳市锦瑞电子有限公司 | Specific protein analyzer and analysis method thereof |
| CN203561641U (en) * | 2013-11-28 | 2014-04-23 | 博奥赛斯（天津）生物科技有限公司 | Chemical luminescence immunity analyzer structure |
| CN104714042A (en) * | 2013-12-16 | 2015-06-17 | 深圳市亚辉龙生物科技有限公司 | Full-automatic chemiluminescence immune analyzer and use method thereof |
| CN104777321A (en) * | 2015-04-16 | 2015-07-15 | 清华大学 | Chemiluminescence immunoassay device |
| CN105628947A (en) * | 2016-02-02 | 2016-06-01 | 徐恩良 | Whole blood autoluminescence immunoassay point-of-care test instrument |
| CN105628687A (en) * | 2016-02-24 | 2016-06-01 | 南京诺尔曼生物技术有限公司 | Full-automatic chemiluminiscence tester |
| CN105628948A (en) * | 2016-03-04 | 2016-06-01 | 深圳普门科技有限公司 | High-speed C reactive protein analyzer and analyzing method thereof |
| CN206339561U (en) * | 2016-07-05 | 2017-07-18 | 深圳普门科技有限公司 | Small electrical chemical illumination immunity analysis instrument |
Cited By (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834114A (en) * | 2017-02-20 | 2017-06-13 | 佛山市金蓝领教育科技有限公司 | A kind of automated detection system of PCR-based application |
| CN107045067A (en) * | 2017-03-27 | 2017-08-15 | 武汉优视科技有限公司 | A kind of liquid-adding device for Western blotting |
| CN114675043A (en) * | 2017-03-28 | 2022-06-28 | 科美诊断技术股份有限公司 | Control method of immunoassay device |
| CN107132345A (en) * | 2017-06-22 | 2017-09-05 | 深圳迈奇生物科技有限公司 | A kind of biological chip reading apparatus |
| CN107132345B (en) * | 2017-06-22 | 2019-03-22 | 中山和芯生物技术有限公司 | A kind of biological chip reading apparatus |
| CN107557356A (en) * | 2017-09-05 | 2018-01-09 | 苏州普川生物科技有限公司 | A kind of small multi-channel library construction instrument and the method for building library |
| CN107557356B (en) * | 2017-09-05 | 2023-06-09 | 南京万承生物科技有限公司 | Small multichannel library construction instrument and library construction method |
| CN108169501A (en) * | 2017-12-04 | 2018-06-15 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
| CN108120840A (en) * | 2018-01-19 | 2018-06-05 | 深圳市亚辉龙生物科技股份有限公司 | Full-automatic immunoblotting assay instrument |
| CN108120840B (en) * | 2018-01-19 | 2020-09-08 | 深圳市亚辉龙生物科技股份有限公司 | Full-automatic immunoblotting analyzer |
| CN108614101A (en) * | 2018-03-14 | 2018-10-02 | 嘉兴科瑞迪医疗器械有限公司 | A kind of magnetic particle Full-automatic chemiluminescence immunoassay analysis meter |
| CN108614101B (en) * | 2018-03-14 | 2021-04-06 | 嘉兴科瑞迪医疗器械有限公司 | Magnetic particle full-automatic chemiluminescence immunoassay analyzer |
| WO2019222445A1 (en) * | 2018-05-18 | 2019-11-21 | Meso Scale Technologies, Llc. | High throughput system for performing assays using electrochemiluminesence including a consumable shaking apparatus |
| US12066449B2 (en) | 2018-05-18 | 2024-08-20 | Meso Scale Technologies, Llc. | High throughput system for performing assays using electrochemiluminescence including a consumable shaking apparatus |
| CN109061208A (en) * | 2018-08-02 | 2018-12-21 | 上海宝中盈仪器仪表有限公司 | Full-automatic biochemical oxygen demand detector |
| CN110967495A (en) * | 2018-09-28 | 2020-04-07 | 深圳市帝迈生物技术有限公司 | Analyzer |
| CN109507438A (en) * | 2018-11-08 | 2019-03-22 | 迪瑞医疗科技股份有限公司 | A kind of linear incubation slot and its automatic clinical chemistry analyzer |
| CN109324200A (en) * | 2018-12-12 | 2019-02-12 | 深圳普门科技股份有限公司 | Sample analyser |
| CN109324200B (en) * | 2018-12-12 | 2022-04-01 | 深圳普门科技股份有限公司 | Sample analyzer |
| CN109342744A (en) * | 2018-12-12 | 2019-02-15 | 深圳普门科技股份有限公司 | A kind of the space layout method and its test method of specific protein analyzer |
| CN109580932A (en) * | 2019-02-26 | 2019-04-05 | 烟台艾德康生物科技有限公司 | A kind of Full-automatic magnetic beads entry joint inspection detection analysis instrument and its application method |
| CN109580932B (en) * | 2019-02-26 | 2019-07-19 | 烟台艾德康生物科技有限公司 | A kind of Full-automatic magnetic beads entry joint inspection detection analysis instrument and its application method |
| CN110040889A (en) * | 2019-05-06 | 2019-07-23 | 安徽科技学院 | A kind of sewage-treatment plant based on heavy metal pollution |
| CN110133310A (en) * | 2019-05-08 | 2019-08-16 | 东软威特曼生物科技（沈阳）有限公司 | Biochemical Analyzer |
| CN110133310B (en) * | 2019-05-08 | 2023-04-25 | 东软威特曼生物科技（沈阳）有限公司 | Biochemical analyzer |
| CN110320353A (en) * | 2019-07-20 | 2019-10-11 | 中国人民解放军总医院 | A kind of fluorescence immunity analyzer and sample testing method applied to altitude environment |
| CN110456089A (en) * | 2019-08-28 | 2019-11-15 | 迈克医疗电子有限公司 | Full-automatic sample analyser and its additional examination agent method, computing device |
| CN110456089B (en) * | 2019-08-28 | 2023-04-25 | 迈克医疗电子有限公司 | Full-automatic sample analyzer, reagent adding method and calculating device thereof |
| CN112578134A (en) * | 2019-09-30 | 2021-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analysis system and control method thereof |
| CN113567690A (en) * | 2020-04-28 | 2021-10-29 | 深圳迎凯生物科技有限公司 | Incubation assembly, incubation device and automatic analysis device |
| WO2021217441A1 (en) * | 2020-04-28 | 2021-11-04 | 深圳迎凯生物科技有限公司 | Incubation assembly, incubation device and automatic analysis device |
| CN111366436A (en) * | 2020-04-30 | 2020-07-03 | 中国人民解放军总医院 | Full-automatic semen processing device |
| CN114814261B (en) * | 2022-04-21 | 2024-07-16 | 华中科技大学 | Automatic chemiluminescent immunoassay chip and detection method thereof |
| CN114814261A (en) * | 2022-04-21 | 2022-07-29 | 华中科技大学 | Automatic chemiluminescence immunoassay chip and detection method thereof |
| CN116660569A (en) * | 2023-07-17 | 2023-08-29 | 中国科学技术大学 | Multifunctional quantitative liquid dripping workstation |
| CN116660569B (en) * | 2023-07-17 | 2023-11-21 | 中国科学技术大学 | Multifunctional quantitative liquid dripping workstation |
| CN117233369A (en) * | 2023-11-15 | 2023-12-15 | 长沙迈迪克智能科技有限公司 | Incubation assembly for medical instrument and medical instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106018784B (en) | 2019-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106018784A (en) | Small electrochemical luminescence immunoassay analyzer and analysis method thereof | |
| CN206339561U (en) | Small electrical chemical illumination immunity analysis instrument | |
| WO2018126773A1 (en) | Automatic analyzer and sample analysis method | |
| WO2018126774A1 (en) | Automatic analysis device and sample analysis method | |
| CN104297498B (en) | A kind of full-automatic biochemical and luminescence immunoassay system | |
| WO2018126775A1 (en) | Automatic analysis device and sample analysis method | |
| JP3521142B2 (en) | How to operate reagents by random access method in an automatic analyzer | |
| CN203337661U (en) | Full-automatic biochemistry and luminescence immunoassay system | |
| CN103969459B (en) | A kind of full-automatic biochemical analysis method | |
| WO2017128806A1 (en) | Automatic fluorescence quantitative immunoassay analyzer and detection method | |
| CN104034908B (en) | A kind of new automatic Biochemical Analyzer | |
| CN106932601B (en) | A kind of Biochemical Analyzer and its detection method | |
| CN116840499A (en) | Automatic analysis device and sample analysis method thereof | |
| CN113125787A (en) | Full-automatic chemiluminescence immunoassay analyzer | |
| CN109107966A (en) | Magnetic bead cleaning device and chemical illumination immunity analysis instrument | |
| CN204347046U (en) | A kind of full-automatic fluorescence immunoassay quantitative analysis device | |
| CN208879198U (en) | Magnetic bead cleaning device and chemical illumination immunity analysis instrument | |
| CN209927873U (en) | Clamping jaw and sampling needle module for chemiluminescence immunoassay analyzer | |
| CN110231472A (en) | A kind of chemical illumination immunity analysis instrument | |
| CN111999512A (en) | Chemiluminescence immunity analyzer | |
| CN114236161A (en) | Liquid suction structure and liquid supply device for immunoassay | |
| CN201917572U (en) | Full-automatic biochemical immunity analysis device | |
| CN209911386U (en) | Chemiluminescence immunity analyzer | |
| CN117990897A (en) | Biochemical immunity all-in-one | |
| CN110244070B (en) | Specific protein analyzer and test method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 518000 A, four floor, Yijing company, 1008, song Bai Road, Nanshan District, Shenzhen, Guangdong, China, four Applicant after: Shenzhen lifotronic Polytron Technologies Inc Address before: 518055 four floor, 15 15 song Bai Road, Nanshan District, Shenzhen, Guangdong. Applicant before: Lifotronic Technology Co., Ltd. |
|
| CB02 | Change of applicant information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Liang Inventor after: Tang Jianbo Inventor after: Liu Xiancheng Inventor after: Xu Yan Inventor after: Li Fei Inventor before: Zhang Liang Inventor before: Tang Jianbo Inventor before: Liu Xiancheng Inventor before: Xu Yan |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |