CN105911198B - A kind of pretreating device of chaff aldehyde material detection and application - Google Patents
A kind of pretreating device of chaff aldehyde material detection and application Download PDFInfo
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- CN105911198B CN105911198B CN201610398175.1A CN201610398175A CN105911198B CN 105911198 B CN105911198 B CN 105911198B CN 201610398175 A CN201610398175 A CN 201610398175A CN 105911198 B CN105911198 B CN 105911198B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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Abstract
The present invention provides the pretreating device and application that chaff aldehyde material detects in a kind of saliva.Described pretreating device is by conical plastic expects pipe（1）, sampling probe（2）, sampling syringe（3）, absorbent material（4）, bottom of which has holes water-absorption material expects pipe（5）, conical plastics lid（6）, absorbent material lid（7）, sampling probe push rod（8）With extraction solution composition, sampling probe connection sampling syringe, sampling syringe connection sampling probe push rod forms sampler；Absorbent material is placed in the water-absorption material expects pipe of bottom of which has holes, water-absorption material expects pipe top is absorbent material lid, thus saliva buffer is formed, sampler and saliva buffer are placed in conical plastic expects pipe, the length of sampling probe reaches below extraction liquid level of solution, and sampling probe push rod and absorbent material lid are above conical plastics lid.
Description
Technical field
The invention belongs to technical field of analytical chemistry, and in particular to a kind of to be exclusively used in saliva before the detection of chaff aldehyde material
Processing unit and application.
Background technology
Furfural, also known as 2 furan carboxyaldehyde, it is same substance with alditol.Its scientific name is furaldehyde, is on 2, furans
The derivative that hydrogen atom is substituted by aldehyde radical；Furfural is to hydrolyze generation pentose under the action of an acid by pentosan, then by dehydration of pentoses
Cyclisation forms；Furfural is the most important derivative of furans ring system, and chemical property is active, can be by the reaction system such as aoxidizing, being condensed
Numerous derivatives is taken, is widely used in the industry such as synthetic plastic, medicine, agricultural chemicals.5 methyl furfural is a kind of food addition
Agent.5 hydroxymethyl furfural is generated by glucose or fructose dehydration, contains a furan nucleus, an aldehyde radical and a hydroxyl first in molecule
Base, its chemical property is more active, can prepare a variety of derivatives by the reaction such as aoxidizing, hydrogenating and be condensed, be important essence
Thin industrial chemicals.
Research for chaff aldehyde material in saliva is advantageous to the property for illustrating health risk when the mankind are exposed to such material
Matter and degree.Due to saliva sample not easily collecting and preservation, chaff aldehyde matter content is relatively low in saliva, and detection is that interference is larger,
Requirement to sample pre-treatments is higher, therefore, exploitation is a kind of be exclusively used in pretreating device that chaff aldehyde material in saliva detects and
Application method is particularly important.
The content of the invention
The pretreating device detected it is an object of the invention to provide chaff aldehyde material in a kind of saliva and application, have fast
Speed, accurate, simple operation and other advantages.
The technical solution adopted in the present invention is：The device by conical plastic expects pipe, sampling probe, sampling syringe, absorbent material,
Water-absorption material expects pipe, conical plastics lid, absorbent material lid, sampling probe push rod and the extraction solution composition of bottom of which has holes, sampling
Pin connection sampling syringe, sampling syringe connection sampling probe push rod form sampler；Absorbent material is placed in the water-absorption material of bottom of which has holes
In expects pipe, water-absorption material expects pipe top is absorbent material lid, thus forms saliva buffer, sampler and saliva buffer are placed in
In conical plastic expects pipe, the length of sampling probe reaches below extraction liquid level of solution, and sampling probe push rod and absorbent material lid are being bored
Above shape plastic tubular cover.Described extraction solution is dichloromethane.
Described conical plastic expects pipe and plastic tubular cover form closed centrifuge tube, for centrifuging and laying in sample, centrifugation
Pipe is 0 ~ 5 mL provided with scale, good airproof performance.
The hole diameter of described bottom of which has holes absorbent material bottom of the tube is 2 ~ 4mm, flowed out for saliva in centrifugation step,
In extraction step extractant add, in drying steps deicer addition.
Described sampling syringe（3）A diameter of 1 ~ 1.5cm.
The application for the pretreating device that chaff aldehyde material detects in saliva：
Absorbent material lid is opened, absorbent material is taken out and is put into smoker oral cavity, it is fast after absorbent material completely moistening
Speed is taken out and puts back to bottom of which has holes water-absorption material expects pipe and be sealed against；After centrifuging 2 ~ 5 min, the saliva sample of clarification is collected into modeling
The bottom of expects pipe；Absorbent material lid is opened, takes absorbent material away, 1 ~ 3 mL dichloromethane extraction solution is added, with bottom
Saliva sample fully mixes, and stands 3 ~ 5 min and is layered as dichloromethane layer and water layer；Sampling probe push rod is pulled out, by 1 ~ 2
ML lower floors dichloromethane extract is taken into sampling syringe, removes sampler, sample is transferred in chromatogram bottle, enters GC/MS points
Analysis, the chaff aldehyde material such as furfural saliva, 5 methyl furfural and 5 hydroxymethyl furfural is detected suitable for simultaneously.
The pretreating device and application that chaff aldehyde material detects in the saliva of the present invention have the positive effect that：
（1）Step is simple, this method only needs to centrifuge, extract after can sample introduction analysis, processing step is simple, while the invention
One-stop pretreating device is provided, it is easy to spread；
（2）Accuracy is high, and this method is handled before being completed in closed system, and step is simple, greatly reduces and treats point
Loss of the thing in transfer process is purified is analysed, adds the accuracy of analysis process；
（3）Cost is relatively low, plastic tube that the device is related to, sampling probe, sampling syringe, absorbent material, bottom of which has holes water suction
Tubes of material, plastic tubular cover, absorbent material lid and sampling probe push rod etc., the material being easy to get for laboratory are prepared, cost compared with
It is low；
（4）It is environment-friendly, device good airproof performance, sample do not allow it is volatile, the invention while precision of analysis is improved,
Reduce pollution of the pretreatment process to environment.
The present invention is used for the detection of chaff aldehyde material in saliva, has quick, accurate, simple operation and other advantages.
Brief description of the drawings
Fig. 1 is the pretreating device structural representation that chaff aldehyde material detects in saliva of the invention.
In figure：1- conical plastics expects pipe, 2- sampling probes, 3- samplings syringe, 4- absorbent materials, 5- bottom of which has holes absorbent materials
Pipe, 6- conical plastics lid, 7- absorbent materials lid, 8- sampling probes push rod, 9- dichloromethane layers, 10- saliva layers.
Embodiment
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright scope.In the examples where no specific technique or condition is specified, according to the technology or condition described by document in the art
Or carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, it is that can be obtained by buying
Conventional products.
The invention will be further described below in conjunction with the accompanying drawings.
Embodiment 1
As shown in figure 1, the pretreating device that chaff aldehyde material detects in the saliva of the present invention, by conical plastic expects pipe 1, sampling
Pin 2, sampling syringe 3, absorbent material 4, the water-absorption material expects pipe 5 of bottom of which has holes, conical plastics lid 6, absorbent material lid 7, take
Sample pin push rod 8 and extraction solution composition, the connection sampling syringe 3 of sampling probe 2, sampling syringe 3 connect sampling probe push rod 8 and form sampling
Device, it can change and unpick and wash as needed；Absorbent material 4 is placed in the water-absorption material expects pipe 5 of bottom of which has holes, and water-absorption material expects pipe top is
Absorbent material lid 7, size match with absorbent material lid 7, thus form saliva buffer, can change and tear open as needed
Wash.Sampler and saliva buffer are placed in conical plastic expects pipe 1, and the length of sampling probe reaches below extraction liquid level of solution, sampling
Pin push rod and absorbent material lid 7 are above conical plastics lid 6.
Described extraction solution is dichloromethane.
Described conical plastic expects pipe 1 and conical plastics lid 6 forms closed centrifuge tube, for centrifuging and laying in sample
Product, centrifuge tube are 0 ~ 5 mL provided with scale, good airproof performance.
The hole diameter of the described bottom of bottom of which has holes water-absorption material expects pipe 5 is 2 ~ 4mm, is flowed for saliva in centrifugation step
Go out, in extraction step extractant add, in drying steps deicer addition.
A diameter of 1 ~ 1.5cm of described sampling syringe 3.
Application Example 1：
Absorbent material lid 7 is opened, absorbent material 4 is taken out and is put into smoker oral cavity, after absorbent material completely moistening,
Rapid take out is put back to bottom of which has holes water-absorption material expects pipe 5 and is sealed against；After centrifuging 2 min, the saliva sample of clarification is collected into
The bottom of plastic tube 1；Absorbent material lid 7 is opened, takes absorbent material 4 away, 2 mL dichloromethane extraction solution is added, with bottom
Saliva sample fully mix, stand 3 min be layered as dichloromethane layer 9 and water layer 10；Sampling probe push rod 8 is pulled out, by 1
ML lower floors dichloromethane extract 9 is taken into sampling syringe 3, removes sampler, sample is transferred in chromatogram bottle, enter GC/MS
Analysis, can detect the chaff aldehyde material such as furfural in saliva, 5 methyl furfural and 5 hydroxymethyl furfural simultaneously.
Application Example 2：
Absorbent material lid 7 is opened, absorbent material 4 is taken out and is put into smoker oral cavity, after absorbent material completely moistening,
Rapid take out is put back to bottom of which has holes water-absorption material expects pipe 5 and is sealed against；After centrifuging 5 min, the saliva sample of clarification is collected into
The bottom of plastic tube 1；Absorbent material lid 7 is opened, takes absorbent material 4 away, 3 mL dichloromethane extraction solution is added, with bottom
Saliva sample fully mix, stand 5 min be layered as dichloromethane layer 9 and water layer 10；Sampling probe push rod 8 is pulled out, by 2
ML lower floors dichloromethane extract 9 is taken into sampling syringe 3, removes sampler, sample is transferred in chromatogram bottle, enter GC/MS
Analysis, can detect the chaff aldehyde material such as furfural in saliva, 5 methyl furfural and 5 hydroxymethyl furfural simultaneously.
Claims (5)
- A kind of 1. pretreating device that chaff aldehyde material detects in saliva, it is characterised in that：The device is by conical plastic expects pipe, sampling Pin, sampling syringe, absorbent material, the water-absorption material expects pipe of bottom of which has holes, conical plastics lid, absorbent material lid, sampling probe push away Bar and extraction solution composition, sampling probe connection sampling syringe, sampling syringe connection sampling probe push rod form sampler；Absorbent material It is placed in the water-absorption material expects pipe of bottom of which has holes, water-absorption material expects pipe top is absorbent material lid, thus forms saliva buffer, takes Sample device and saliva buffer are placed in conical plastic expects pipe, and the length of sampling probe reaches below extraction liquid level of solution, sampling probe push rod With absorbent material lid above conical plastics lid.
- 2. the pretreating device that chaff aldehyde material detects in saliva according to claim 1, it is characterised in that：Described cone Shape expects pipe and conical plastics lid forms closed centrifuge tube, for centrifuging and laying in sample, centrifuge tube is provided with scale For 0 ~ 5 mL, good airproof performance.
- 3. the pretreating device that chaff aldehyde material detects in saliva according to claim 1, it is characterised in that described bottom The hole diameter of portion's absorbent material bottom of the tube with holes is 2 ~ 4mm.
- 4. the pretreating device that chaff aldehyde material detects in saliva according to claim 1, it is characterised in that：Described takes A diameter of 1 ~ 1.5cm of sample syringe.
- 5. the application for the pretreating device that chaff aldehyde material detects in the saliva described in a kind of claim 1, it is characterised in that：Beat Absorbent material lid is opened, absorbent material is taken out and is put into smoker oral cavity, after absorbent material completely moistening, rapid take out is put back to Bottom of which has holes water-absorption material expects pipe is simultaneously sealed against；After centrifuging 2 ~ 5 min, the saliva sample of clarification is collected under plastic tube Portion；Absorbent material lid is opened, takes absorbent material away, adds 1 ~ 3 mL dichloromethane extraction solution, the saliva sample with bottom Fully mix, stand 3 ~ 5 min and be layered as dichloromethane layer and water layer；Sampling probe push rod is pulled out, by 1 ~ 2 mL lower floors two Chloromethane alkane extract is taken into sampling syringe, removes sampler, sample is transferred in chromatogram bottle, is entered GC/MS analyses, is applied to Detect furfural in saliva, 5 methyl furfural and 5 hydroxymethyl furfural chaff aldehyde material simultaneously.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610398175.1A CN105911198B (en) | 2016-06-07 | 2016-06-07 | A kind of pretreating device of chaff aldehyde material detection and application |
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| CN201610398175.1A CN105911198B (en) | 2016-06-07 | 2016-06-07 | A kind of pretreating device of chaff aldehyde material detection and application |
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| CN105911198A CN105911198A (en) | 2016-08-31 |
| CN105911198B true CN105911198B (en) | 2018-03-09 |
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Families Citing this family (2)
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| CN111024906A (en) * | 2019-12-26 | 2020-04-17 | 光明乳业股份有限公司 | Method for rapidly evaluating content of furfural substances in solid dairy product based on storage temperature and humidity of solid dairy product |
| CN111024907A (en) * | 2019-12-26 | 2020-04-17 | 光明乳业股份有限公司 | Method for rapidly evaluating content of furfural substances in liquid dairy product based on storage temperature and humidity of liquid dairy product |
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| US7387899B1 (en) * | 1995-07-11 | 2008-06-17 | D Angelo Joseph P | Saliva sample collection system |
| CN101762647A (en) * | 2008-12-26 | 2010-06-30 | 青岛啤酒股份有限公司 | Method for measuring 2-furfural in beer by using high performance liquid chromatography |
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| US7387899B1 (en) * | 1995-07-11 | 2008-06-17 | D Angelo Joseph P | Saliva sample collection system |
| EP1006359A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Device and method for separating components of a liquid sample |
| WO2001070403A1 (en) * | 2000-03-17 | 2001-09-27 | Greiner Bio-One Gmbh | Collection vessel for separating biological fluid constituents |
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| CN104142372A (en) * | 2014-07-17 | 2014-11-12 | 中国石油化工股份有限公司 | Method for detecting furfuryl alcohol content in furfural biological hydrogenation reaction liquid |
| CN104215474A (en) * | 2014-09-03 | 2014-12-17 | 东南大学 | Two-pipe saliva sampling device |
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| CN105911198A (en) | 2016-08-31 |
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