CN105779166A - Preparation method of low-foaming multienzyme cleaning agent for medical apparatus and instruments - Google Patents
Preparation method of low-foaming multienzyme cleaning agent for medical apparatus and instruments Download PDFInfo
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- CN105779166A CN105779166A CN201610095453.6A CN201610095453A CN105779166A CN 105779166 A CN105779166 A CN 105779166A CN 201610095453 A CN201610095453 A CN 201610095453A CN 105779166 A CN105779166 A CN 105779166A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000012459 cleaning agent Substances 0.000 title claims abstract description 24
- 238000005187 foaming Methods 0.000 title abstract 3
- 238000003756 stirring Methods 0.000 claims abstract description 35
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 229940088598 enzyme Drugs 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000003381 stabilizer Substances 0.000 claims abstract description 14
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000008367 deionised water Substances 0.000 claims abstract description 13
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 13
- 230000007935 neutral effect Effects 0.000 claims abstract description 12
- 239000003755 preservative agent Substances 0.000 claims abstract description 10
- 230000002335 preservative effect Effects 0.000 claims abstract description 10
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 7
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 7
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 7
- 239000004382 Amylase Substances 0.000 claims abstract description 6
- 102000013142 Amylases Human genes 0.000 claims abstract description 6
- 108010065511 Amylases Proteins 0.000 claims abstract description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 6
- 235000019418 amylase Nutrition 0.000 claims abstract description 6
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 6
- 108010059892 Cellulase Proteins 0.000 claims abstract description 5
- 229940106157 cellulase Drugs 0.000 claims abstract description 5
- 108010014251 Muramidase Proteins 0.000 claims abstract description 4
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 4
- 239000004325 lysozyme Substances 0.000 claims abstract description 4
- 229960000274 lysozyme Drugs 0.000 claims abstract description 4
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 4
- 239000004367 Lipase Substances 0.000 claims abstract description 3
- 102000004882 Lipase Human genes 0.000 claims abstract description 3
- 108090001060 Lipase Proteins 0.000 claims abstract description 3
- 235000019421 lipase Nutrition 0.000 claims abstract description 3
- 102000016943 Muramidase Human genes 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims description 23
- 239000006185 dispersion Substances 0.000 claims description 20
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 7
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 7
- 239000004327 boric acid Substances 0.000 claims description 7
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 7
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 7
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims description 6
- 108010053775 Nisin Proteins 0.000 claims description 6
- 239000004309 nisin Substances 0.000 claims description 6
- 235000010297 nisin Nutrition 0.000 claims description 6
- 102000035195 Peptidases Human genes 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- -1 chelating agen Substances 0.000 claims description 2
- 239000001509 sodium citrate Substances 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 abstract description 10
- 239000004365 Protease Substances 0.000 abstract description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 3
- 239000002738 chelating agent Substances 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 239000011259 mixed solution Substances 0.000 abstract 4
- 239000002131 composite material Substances 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 239000007921 spray Substances 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 239000003344 environmental pollutant Substances 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 102100033468 Lysozyme C Human genes 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 2
- 101710084378 Lipase 2 Proteins 0.000 description 1
- 101710084376 Lipase 3 Proteins 0.000 description 1
- 101710084369 Lipase 4 Proteins 0.000 description 1
- 101710132682 Lysozyme 1 Proteins 0.000 description 1
- 101710132699 Lysozyme 2 Proteins 0.000 description 1
- 101710132698 Lysozyme 3 Proteins 0.000 description 1
- 102100026848 Lysozyme-like protein 2 Human genes 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 108010091371 endoglucanase 1 Proteins 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D11/00—Special methods for preparing compositions containing mixtures of detergents
- C11D11/0094—Process for making liquid detergent compositions, e.g. slurries, pastes or gels
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/37—Polymers
- C11D3/3703—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C11D3/3707—Polyethers, e.g. polyalkyleneoxides
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/02—Inorganic compounds
- C11D7/04—Water-soluble compounds
- C11D7/08—Acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/261—Alcohols; Phenols
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/265—Carboxylic acids or salts thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/268—Carbohydrates or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/32—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/32—Organic compounds containing nitrogen
- C11D7/3218—Alkanolamines or alkanolimines
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/14—Hard surfaces
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Emergency Medicine (AREA)
- Inorganic Chemistry (AREA)
- Molecular Biology (AREA)
- Detergent Compositions (AREA)
Abstract
The invention belongs to the technical field of cleaning and disinfection of medical apparatus and instruments and particularly discloses a preparation method of a low-foaming multienzyme cleaning agent for medical apparatus and instruments.The preparation method comprises the following steps of 1, adding polyethylene glycol, a chelating agent, preservative and an enzyme composite stabilizing agent to deionized water, and stirring the mixture uniformly, so that a first mixed solution is obtained; 2, sequentially adding neutral hydrolysis protease and marine organism protease to isopropyl alcohol, and stirring the mixture uniformly, so that a second mixed solution is obtained; 3, adding the second mixed solution to the first mixed solution, conducting stirring, then sequentially adding lipase, amylase, neutral cellulase and lysozyme, conducting stirring, finally adding trehalose, and continuing stirring.By means of the multienzyme cleaning agent prepared through the preparation method, biomembranes on the surfaces of the apparatus and instruments can be removed, after cleaning is completed, the surfaces of the apparatus and instruments are good in brightness and free of residual spots, and the low-foaming multienzyme cleaning agent is applicable to a fully-automatic spray cleaning machine or ultrasonic medical cleaning equipment.
Description
Technical field
The invention belongs to the cleaning and sterilizing technical field of medical apparatus and instruments, the preparation method being specifically related to a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments.
Background technology
It is known that, medical apparatus and instruments in use can be infected with the dirts such as blood, mucosal tissue or greasy dirt, if cleaning thorough not, can cause that serious pathogenic bacteria grows or pollute corrosion drawback, thus bringing the service life threatening and reducing equipment to the healthy of people.Chemical traditional in the market is common also exists the problems such as consumption is big, pollute greatly, cleaning is not thorough, and medical apparatus and instruments has corrosivity, causes that automatic rinser is easily damaged.
And it has been discovered that some enzyme has the ability of good decomposing pollutant, its mechanism is in that these enzyme can react with greasy dirt or pollutant, thus decomposing to fall by pollutant.
Patent CN104073367A discloses a kind of Multi-enzyme detergent being applicable to machine washing medical apparatus and instruments and preparation method thereof, although its preparation method is relatively simple, yet with the protease in its formula can other biological enzyme in decomposing solution, therefore cause that the bioenzyme activity in abluent is low.
Summary of the invention
The preparation method that it is an object of the invention to provide a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments.
The technical scheme realizing the object of the invention is: the preparation method of a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments, and preparation process is as follows:
1) add Polyethylene Glycol, chelating agen, preservative and enzyme combining stabilizer in deionized water, stir, prepare the first mixed liquor；
2) in isopropanol, it is sequentially added into neutral water proteolytic enzyme, marine protein enzyme, stirs, prepare the second mixed liquor；
3) the second mixed liquor is added in the first mixed liquor, stir 10～30 minutes, then be sequentially added into lipase, amylase, neutral cellulase and lysozyme in order, stir 10～30 minutes, be eventually adding trehalose and continue stirring 10～30 minutes.
Preferably, described enzyme combining stabilizer, composed as follows by weight: triethanolamine 5～15 parts, boric acid 5～15 parts, sucrose 5～15 parts, sodium carboxymethyl cellulose 1～5 part, deionized water 100 parts.
It is further preferred that described enzyme combining stabilizer is prepared by following methods step: 1) sodium carboxymethyl cellulose, triethanolamine and sucrose are dissolved in deionized water, prepare the first dispersion liquid；2) boric acid is dissolved in ionized water, prepares the second dispersion liquid；3) the second dispersion liquid is slowly added dropwise in the first dispersion liquid, stirs.
It is furthermore preferred that described chelating agen is sodium citrate or EDTA-4Na, described preservative is biological preparation nisin.
Compared with prior art, the technological merit of the present invention is in that:
1) present invention does not contain surfactant in the process preparing low bubble multienzyme cleaning agent, effectively reduce chemical substance residual on apparatus, alcohols material in formula and liquid enzymes are by, after suitable proportioning, making Multi-enzyme detergent reach a kind of intimate still state.Multienzyme cleaning agent prepared by the present invention can remove apparatus surface biological film, equipment surfaces good brightness, noresidue speckle after cleaning, it is adaptable to fully-automatic sprinkling cleaning machine or ultrasound medical cleaning equipment.
2) with the addition of the compositions such as enzyme combining stabilizer in the low bubble multienzyme cleaning agent formula of the present invention, effectively extend enzymatic activity in multienzyme cleaning agent, the enzyme liquid after multienzyme cleaning agent dilution can preserve 18h, and the enzymatic activity of tradition enzyme cleanout fluid inactivates at about 4h.
3) the low bubble multienzyme cleaning agent formula of the present invention with the addition of lysozyme so that multienzyme cleaning agent has quickly makes virally inactivated pharmacological action, and enzyme liquid is served good protective effect by the trehalose additionally added.
4) preservative in the low bubble multienzyme cleaning agent formula that the present invention adds is biological preparation nisin, has the advantages such as safe and environment-friendly compared with common chemically composited preservative.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described through.
Embodiment 1
1) preparation of enzyme combining stabilizer
Sodium carboxymethyl cellulose 3 parts, triethanolamine 5 parts and sucrose 15 parts are dissolved in deionized water, prepare the first dispersion liquid；Boric acid 10 parts is dissolved in ionized water 100 parts, prepares the second dispersion liquid；Second dispersion liquid is slowly added dropwise in the first dispersion liquid, stirs.
2) preparation of low bubble multienzyme cleaning agent
1) in deionized water 100 parts, add Polyethylene Glycol 5 parts, 4 parts of chelator acid citrate sodium, preservative biological preparation nisin 0.3 part and enzyme combining stabilizer 10 parts, stir, prepare the first mixed liquor；
2) in isopropanol 15 parts, it is sequentially added into neutral water proteolytic enzyme 5 parts, marine protein enzyme 4 parts, stirs, prepare the second mixed liquor；
3) the second mixed liquor is added in the first mixed liquor, stir 10 minutes, then be sequentially added into lipase 2 parts, amylase 1 part, neutral cellulase 1 part and lysozyme 3 parts in order, stir 10 minutes, be eventually adding trehalose 5 parts and continue stirring 10 minutes.
Embodiment 2
1) preparation of enzyme combining stabilizer
Sodium carboxymethyl cellulose 1 part, triethanolamine 10 parts and sucrose 5 parts are dissolved in deionized water, prepare the first dispersion liquid；Boric acid 15 parts is dissolved in ionized water 100 parts, prepares the second dispersion liquid；Second dispersion liquid is slowly added dropwise in the first dispersion liquid, stirs.
2) preparation of low bubble multienzyme cleaning agent
1) in deionized water 100 parts, add Polyethylene Glycol 15 parts, 6 parts of chelator acid citrate sodium, preservative biological preparation nisin 0.1 part and enzyme combining stabilizer 5 parts, stir, prepare the first mixed liquor；
2) in isopropanol 10 parts, it is sequentially added into neutral water proteolytic enzyme 3 parts, marine protein enzyme 6 parts, stirs, prepare the second mixed liquor；
3) the second mixed liquor is added in the first mixed liquor, stir 20 minutes, then be sequentially added into lipase 3 parts, amylase 1.5 parts, neutral cellulase 0.8 part and lysozyme 2 parts in order, stir 20 minutes, be eventually adding trehalose 3 parts and continue stirring 20 minutes.
Embodiment 3
1) preparation of enzyme combining stabilizer
Sodium carboxymethyl cellulose 5 parts, triethanolamine 15 parts and sucrose 10 parts are dissolved in deionized water, prepare the first dispersion liquid；Boric acid 5 parts is dissolved in ionized water 100 parts, prepares the second dispersion liquid；Second dispersion liquid is slowly added dropwise in the first dispersion liquid, stirs.
2) preparation of low bubble multienzyme cleaning agent
1) in deionized water 100 parts, add Polyethylene Glycol 20 parts, chelating agen EDTA-4Na3 part, preservative biological preparation nisin 0.5 part and enzyme combining stabilizer 8 parts, stir, prepare the first mixed liquor；
2) in isopropanol 5 parts, it is sequentially added into neutral water proteolytic enzyme 6 parts, marine protein enzyme 3 parts, stirs, prepare the second mixed liquor；
3) the second mixed liquor is added in the first mixed liquor, stir 30 minutes, then be sequentially added into lipase 4 parts, amylase 2 part, neutral cellulase 0.5 part and lysozyme 1 part in order, stir 30 minutes, be eventually adding trehalose 0.5 part and continue stirring 30 minutes.
Claims (5)
1. the preparation method for the low bubble multienzyme cleaning agent of medical apparatus and instruments, it is characterised in that preparation process is as follows:
Add Polyethylene Glycol, chelating agen, preservative and enzyme combining stabilizer in deionized water, stir, prepare the first mixed liquor；
Isopropanol is sequentially added into neutral water proteolytic enzyme, marine protein enzyme, stirs, prepare the second mixed liquor；
Second mixed liquor is added in the first mixed liquor, stir 10～30 minutes, then be sequentially added into lipase, amylase, neutral cellulase and lysozyme in order, stir 10～30 minutes, be eventually adding trehalose and continue stirring 10～30 minutes.
2. the preparation method of a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments according to claim 1, it is characterized in that: described enzyme combining stabilizer, composed as follows by weight: triethanolamine 5～15 parts, boric acid 5～15 parts, sucrose 5～15 parts, sodium carboxymethyl cellulose 1～5 part, deionized water 100 parts.
3. the preparation method of a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments according to claim 2, it is characterized in that: described enzyme combining stabilizer is prepared by following methods step: 1) sodium carboxymethyl cellulose, triethanolamine and sucrose are dissolved in deionized water, prepare the first dispersion liquid；2) boric acid is dissolved in ionized water, prepares the second dispersion liquid；3) the second dispersion liquid is slowly added dropwise in the first dispersion liquid, stirs.
4. the preparation method of a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments according to claim 1, it is characterised in that: described chelating agen is sodium citrate or EDTA-4Na.
5. the preparation method of a kind of low bubble multienzyme cleaning agent for medical apparatus and instruments according to claim 1, it is characterised in that: described preservative is biological preparation nisin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610095453.6A CN105779166A (en) | 2016-02-22 | 2016-02-22 | Preparation method of low-foaming multienzyme cleaning agent for medical apparatus and instruments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610095453.6A CN105779166A (en) | 2016-02-22 | 2016-02-22 | Preparation method of low-foaming multienzyme cleaning agent for medical apparatus and instruments |
Publications (1)
| Publication Number | Publication Date |
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| CN105779166A true CN105779166A (en) | 2016-07-20 |
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Cited By (5)
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| CN106635461A (en) * | 2016-11-29 | 2017-05-10 | 长安大学 | Medical instrument cleaner and preparation method thereof |
| CN111394196A (en) * | 2020-04-12 | 2020-07-10 | 长沙汇泉环境科技有限公司 | Stone stain cleaning agent and preparation method thereof |
| CN111764174A (en) * | 2019-04-01 | 2020-10-13 | 深圳市芭格美生物科技有限公司 | Non-woven fabric containing enzyme and preparation method thereof |
| CN114989899A (en) * | 2022-07-18 | 2022-09-02 | 安徽亿人安股份有限公司 | Medical alkaline multienzyme cleaning agent and application thereof |
| CN115445443A (en) * | 2022-10-20 | 2022-12-09 | 杭州佳嘉乐生物技术有限公司 | Enzyme cleaning agent of ultrafiltration nanofiltration membrane for orlistat production and application thereof |
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| CN106635461A (en) * | 2016-11-29 | 2017-05-10 | 长安大学 | Medical instrument cleaner and preparation method thereof |
| CN111764174A (en) * | 2019-04-01 | 2020-10-13 | 深圳市芭格美生物科技有限公司 | Non-woven fabric containing enzyme and preparation method thereof |
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Application publication date: 20160720 |