CN105494451B - A kind of algicide and its preparation method and application - Google Patents
A kind of algicide and its preparation method and application Download PDFInfo
- Publication number
- CN105494451B CN105494451B CN201610042162.0A CN201610042162A CN105494451B CN 105494451 B CN105494451 B CN 105494451B CN 201610042162 A CN201610042162 A CN 201610042162A CN 105494451 B CN105494451 B CN 105494451B
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- Prior art keywords
- algicide
- algae
- methanol
- grape
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- 239000003619 algicide Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims description 4
- 241000219095 Vitis Species 0.000 claims abstract description 18
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 18
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 18
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 18
- 239000000401 methanolic extract Substances 0.000 claims abstract description 18
- 239000004480 active ingredient Substances 0.000 claims abstract description 5
- 241000195493 Cryptophyta Species 0.000 claims description 35
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 238000009966 trimming Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 230000006378 damage Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 206010003497 Asphyxia Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 230000001550 time effect Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of algicide, the active ingredient of the algicide is the methanolic extract of grape leave.The algicide of the present invention, takes full advantage of the biotic component in grape leave, can significantly inhibit the growth of common alga cells.Technical scheme is not only avoided environmental pollution, and is had good ecological benefits and economic benefit using the discarded grape leaf after grape trimming.
Description
Technical field
The present invention relates to a kind of algicide, belong to algae removing and prevention and control field.
Background technology
A large amount of sanitary sewages and industrial wastewater discharge rich in N, P are pooled to ocean to rivers and lakes, make water body
Eutrophication causes algae and the rapid amount reproduction of other planktonic organisms, results in serious environmental hazard, this phenomenon is in recent years
Frequently to occur in the major small lakes in China, river and marine site etc..The amount reproduction of algae not only seriously hinders the water surface and leads to
Row, and water body environment is seriously destroyed, ecological environment security is endangered, main harm has：(1) cause water hypoxia, make fish
Wait aquatiles hypoxia death；(2) algae floats on lake surface, shielded from sunlight, makes sunlight that aquatic organism cannot be sufficient and shadow
Ring its existence and breeding；(3) algae secretion or generation mucus, attach on the cheek of the aquatic animals such as fish, interfere breathing, cause
Death by suffocation；(4) algae toxin is secreted, algae toxin can damage nervous system, cause numb convulsions, dispute, expiratory dyspnea or even exhale
Failure is inhaled, also results in the damage of the organs such as diarrhea, vomiting, liver kidney, has and promotees the effects that knurl is carcinogenic.In addition, algae can also influence
Water supply quality simultaneously increases water producing cost, and excessive algae can bring obstacle, it is necessary to improve and increase to water factory processed in filter process
Add filtering measure.
In view of the above-mentioned problems, how to carry out having become the research hotspot of field of Environment Protection except algae.At present, algae side is removed both at home and abroad
Method mainly has Physical, chemical method and bioanalysis three categories.Physical method is the algae removed by manual work on the water surface, no
The nutriment of enrichment, which can fundamentally be solved, stimulates the effect of algal grown, and need to expend substantial amounts of man power and material into
Row is removed repeatedly, and cost is higher；Chemical algae removing agent has time effect that is short, quick, can quickly killing algae, but at present
Chemical algae removing drug can be enriched with, amplify in vivo, so as to the serious influence ecosystem；Biological algae removal mainly passes through
The pathogenic microorganism of algae is carried out except algae, but pathogen generates variation and other biological group can be generated greatly under field conditions (factors)
Ground influences, while the effect of pathogen has very big uncertainty.
The content of the invention
In view of the deficiencies of the prior art, the invention discloses a kind of algicide, the algicide is to come from the day of grape leave
Right ingredient is carried out for active material except algae, can not only effectively kill and inhibit the growth of algae, and clear efficacy is stablized, to ring
Border is without side-effects, has good ecological benefits and economic value.
Specifically, the present invention is achieved through the following technical solutions：
A kind of algicide, active ingredient are the methanolic extract of grape leave.
The experiment that applicant carries out shows that the methanolic extract of grape leave can substantially inhibit frustule growth.
In above-mentioned, applicant has used this saying of active ingredient, it will be appreciated by those skilled in the art that in actual system
During into commercially available finished product, often also need to add in type, the use of other shaping auxiliary materials or other auxiliary agents, these auxiliary materials and auxiliary agent
Amount etc., without special difference, can be universally used in the algicide of the present invention with the other similar formulations in this field.
Wherein, in algicide, extract concentrations are not less than 100mg/ml.The algicide of higher concentration is conducive to except algae
There is the killing scope of bigger in dilution.If extract concentrations are too low, it is unfavorable for giving full play to its inhibition frustule life
Long function.
Correspondingly, the invention discloses the preparation methods of the algicide, include the following steps：
1) grape leave of drying, is taken, methanol solution is added in after being fully ground, extracts first under room temperature, dark condition
Secondary methanol extract liquid；
2) the first methanol extract liquid of gained, is taken, gained methanol extract liquid is distilled in a heated condition, until by first
Alcohol has steamed, and water is added to adjust to aimed concn.
In above-mentioned, the relative usage of grape leave, methanol solution etc. can be adjusted with production environment as needed, first
Heating temperature etc. of alcohol distillation can the existing experiment of root root organic chemistry filed etc. be adjusted.
In general, in step 1), the dosage of grape leave and methanol solution causes first methanol extract liquid concentration to be not less than
100mg/ml；In step 2), aimed concn is not less than 100mg/ml for extract concentrations.
By controlling the concentration of each step, be conducive to simplify packaging and the application of subsequent product.
Further, the algicide the invention also discloses the present invention removes algae except the application in algae using the present invention
Agent carries out the method except algae, is that algicide is added in algae solution.
After algicide is added in algae solution, the too low growth for being unfavorable for inhibiting alga cells of active ingredient concentration is excellent
Choosing, in algae solution, for the methanolic extract concentration of grape leave not less than 12mg/ml, processing time is not less than 12h.
The experiment that applicant carries out in a variety of algae is shown, is used to inhibit and kill common using the algicide of the present invention
Algae, after handling 12h, inhibiting rate is not less than 50%；Inhibiting rate reaches 100% after processing 48h.
Specific embodiment
The specific reality of the present invention is being provided below in specific implementation and its application effect in order to illustrate the present invention, applicant
Apply example and its application effect.It will be understood by those skilled in the art that this is only illustrative, and it is non-limiting.Embodiment 1
It 1. weighing the grape leave of 5g drying, is fully ground, adds in 50% methanol of 50ml and carried under room temperature, dark condition
Take 48h, extract concentration 100mg/ml；
2. measuring methanol extract liquid 40ml, methanol extract liquid is distilled under the conditions of 64.5 DEG C, until methanol is steamed
It is complete, and water is added to mend to 40ml.
3. the distillate is added to cell density as 4 × 106Chlamydomonas solution in, make its final concentration of 12mg/ml.Place
After managing 12h, inhibiting rate reaches 59% compared with the control；After 48h, frustule is all dead.Embodiment 2
It 1. weighing the grape leave of 20g drying, is fully ground, adds in 50% methanol of 100ml under room temperature, dark condition
Extract 48h, extract concentration 200mg/ml；
2. measuring methanol extract liquid 50ml, methanol extract liquid is distilled, until methanol has been steamed, and add water mend to
100ml。
3. the distillate is added to cell density as 4 × 106Cyanobacteria solution in, make its final concentration of 20mg/ml.Place
After managing 12h, inhibiting rate reaches 53% compared with the control；After 48h, frustule is all dead.Embodiment 3
It 1. weighing the grape leave of 5g drying, is fully ground, adds in 50% methanol of 50ml and carried under room temperature, dark condition
Take 48h, extract concentration 100mg/ml；
2. measuring methanol extract liquid 40ml, methanol extract liquid is distilled, until methanol has been steamed, and add water mend to
40ml。
3. the distillate is added to cell density as 4 × 106Chlorella solution in, make its final concentration of 15mg/ml.
After handling 12h, inhibiting rate reaches 55% compared with the control；After 48h, frustule is all dead.
The distillate of embodiment 1 is added to cell density as 4 × 106Chlamydomonas solution in, and make its concentration be 12mg/
Ml, respectively in the growing state for handling 12h, measuring cell with 48h for 24 hours.To add in isometric distilled water as control.
Table 1 removes algae effect experiment
1 data of table and conclusion show：Algicide prepared by the blade discarded after being trimmed using grape, can be apparent in 12h
Inhibition frustule growth；After handling 48h, complete cell death can be caused.
Claims (2)
- A kind of 1. method carried out using algicide except algae, it is characterised in that algicide is added in algae solution, it is described to remove algae The active ingredient of agent is the methanolic extract of grape leave, and preparation method includes the following steps：1) grape leave of drying, is taken, Methanol solution is added in after being fully ground, first methanol extract liquid is extracted to obtain under room temperature, dark condition；2) the first first of gained, is taken Alcohol extract in a heated condition distills gained methanol extract liquid, until methanol has been steamed, water is added to adjust to target rich Degree.
- 2. a kind of method carried out using algicide except algae according to claim 1, it is characterised in that in algae solution, Portugal The methanolic extract concentration of grape blade is not less than 12mg/ml, and processing time is not less than 12h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610042162.0A CN105494451B (en) | 2016-01-21 | 2016-01-21 | A kind of algicide and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610042162.0A CN105494451B (en) | 2016-01-21 | 2016-01-21 | A kind of algicide and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105494451A CN105494451A (en) | 2016-04-20 |
| CN105494451B true CN105494451B (en) | 2018-05-25 |
Family
ID=55703064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610042162.0A Expired - Fee Related CN105494451B (en) | 2016-01-21 | 2016-01-21 | A kind of algicide and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105494451B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954643B (en) * | 2017-04-26 | 2019-06-04 | 南华大学 | The method that algistatic activity ingredient prepares algae-inhibiting agent is extracted from grape pip |
| CN113068713A (en) * | 2021-03-30 | 2021-07-06 | 江苏曌之源实业有限公司 | Efficient algae remover and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101623012A (en) * | 2009-07-31 | 2010-01-13 | 四川大学 | Plant leaching solution based compound algistat |
-
2016
- 2016-01-21 CN CN201610042162.0A patent/CN105494451B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101623012A (en) * | 2009-07-31 | 2010-01-13 | 四川大学 | Plant leaching solution based compound algistat |
Non-Patent Citations (4)
| Title |
|---|
| "不同提取条件对葡萄叶提取物抑制";袁茜 等，;《植物保护》;20090408;第35卷(第2期);第125-127页 * |
| "微波法辅助提取葡萄叶白藜芦醇及其";孙磊磊 等，;《食品工业科技》;20151015;第36卷(第20期);第271-277、283页 * |
| "抑制茄子黄萎菌的植物提取物的筛选";周璇 等，;《生态学杂志》;20070815;第26卷(第8期);第1217-1221页 * |
| "葡萄叶中总黄酮的提取工艺研究";张纵圆 等，;《生物技术》;20071215;第17卷(第6期);第58-60页 * |
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| Publication number | Publication date |
|---|---|
| CN105494451A (en) | 2016-04-20 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180525 Termination date: 20190121 |
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