CN105199964A - Oxygen-pumped and automatic constant-temperature liquid strain fermenting device - Google Patents
Oxygen-pumped and automatic constant-temperature liquid strain fermenting device Download PDFInfo
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- CN105199964A CN105199964A CN201510505601.2A CN201510505601A CN105199964A CN 105199964 A CN105199964 A CN 105199964A CN 201510505601 A CN201510505601 A CN 201510505601A CN 105199964 A CN105199964 A CN 105199964A
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Abstract
The invention discloses an oxygen-pumped and automatic constant-temperature liquid strain fermenting device. The oxygen-pumped and automatic constant-temperature liquid strain fermenting device specifically comprises a constant temperature pool, a culture bottle and an air pump; the constant temperature pool is filled with clear water, and the culture bottle is placed in the constant temperature pool; the constant temperature pool is composed of a temperature-controlled instrument, a heating pipe and a temperature probe, the culture bottle is composed of a bottle plug, an air inlet pipe and a filter, the air pump and the air inlet pipe are connected together through the filter and a rubber pipe, oxygen is input into the culture bottle through the air pump, and mycelia (pellets) of liquid strains cultured in the bottle continuously metabolize and are differentiated more and more; the temperature-controlled instrument controls the heating pipe to heat water in the constant temperature pool and keep the water temperature within a certain range, the liquid strains in the culture bottle are cultured in the optimal temperature environment, and the strains are short in culturing period and high in quality. The oxygen-pumped and automatic constant-temperature liquid strain fermenting device is little in investment, quick in yield returns, easy and convenient to operate and very suitable for being applied to small edible fungus plants, individual households and rural families.
Description
Technical field
The invention belongs to a kind of device producing edible fungi liquid strain, be specifically related to the liquid fungus seed device of a kind of logical oxygen and automatic constant-temperature.
Background technology
Edible fungi liquid strain has with short production cycle, cell age neat and consistent, inoculation is convenient, fermentation is fast, be suitable for the advantages such as factorial praluction, and it thoroughly finishes China's Edible Fungi and has continued to use the history that more than one thousand years adopts solid spawn, is a qualitative leap.At present, shaking table and fermentor tank two kinds is mainly contained for the production of edible fungi liquid strain installations and facilities.Fermentor tank not only involves great expense, but also it is numerous and diverse to have application process, consume energy high, the shortcoming such as have a big risk, general mushroom agriculture is difficult to bear, when liquid spawn produced by shaking table, because the reasons in structure of equipment limits its turnout.
Application number is " Simple liquid strain production device " of 89203999.X, and the feature of this utility model is that this device has ultraclean filters, inoculator and multistage air cleaner, all adopts closure pipeline to produce.This utility model nutrient solution used does not need whole autoclaving, just can carry out closed cultured continuously.
Application number is " without the shaking-table edible liquid bacteria fertilizer " of 94216524.1, and this this device comprises primary fermentation culture apparatus, middle rank expands fermentation culture device and whole level expands fermentation culture device.The basic structure form of fermentation unit at different levels is: breather is connected with air filter by flexible pipe, and strainer is connected with ventpipe by flexible pipe, and ventpipe is filled by bottleneck cotton and inserts in culturing bottle.And the strainer that middle rank and whole level expand fermentation culture device is connected with barometric pipe by flexible pipe and Y-tube, and communicate with flow diverter.
Application number is " Simple apparatus for culturing liquid strain in depth layer " of 87210089, this device is by easy air bag or the little air pump air feed of electromagnetism, the ventilation of ventilation head, short hard tube and flexible pipe, the bulb strainer of tolerance adjustable filtering device and the common cotton of filling is filtered gas and is fermentation unit composition with simple and easy mash-back.It instead of without oil gas press, air water separator, metal-air strainer and glass fermentation flask, get rid of shaking table, cost is reduced to about hundred yuan greatly, operation not necessarily needs power supply and autoclave sterilization Autoclave when running, this be vast rural area edible mushrooms specialist or field microorganism scientific research provide afford, the simple and easy reliable strain cultivation that can use and production equipment.
Application number is " Simple liquid strain fermenter " of 200920244525.4, this utility model primarily of: oil-less pump, first, second and third sebific duct, first, second, third and fourth filter ball, first, second and third ventpipe, first and second cylinder of steel, first and second filler, venting port, connect bacterium mouth, fermentor tank, web plate form.First filter ball one end is connected with oil-less pump, the other end is connected with the first cylinder of steel, second filter ball one end is connected with the first cylinder of steel, the other end is connected with the second cylinder of steel, 3rd filter ball one end is connected with the second cylinder of steel, the other end is connected with the 3rd ventpipe by the 4th sebific duct, the 4th filter ball, and the 3rd ventpipe is connected with fermentor tank, fermentor tank is equipped with venting port and connects bacterium mouth.
Application number is " a kind of Simple device for culturing liquid strain " of 200820160881.3, this device is by vial (1), soft rubber ball (2), Glass tubing (3), glass bulb (4) and connecting hose (5) form.It is characterized in that: the diameter at butt end of soft rubber ball (2) is larger than glass bottle opening, soft rubber ball (2) is upper makes a call to 2 holes, inserts Glass tubing (3) and glass bulb (4).Glass tubing (3) is inlet pipe, and length is a little more than the height of vial, and upper end silicone tube (5) connects glass bulb (4), and lower end is through soft rubber ball, and port is from 1-2 centimetre at the bottom of bottle.Fill, so that filtrated air with clean cotton in glass bulb (4).
Application number is " Simplified fermentor for producing liquid spawn " of 200520079434.1, this device adopts saline bottle to make fermenting container, on the bottleneck convex edge of saline bottle, cover has rubber ring, rubber ring has tapped aglet at the hypomere cover of bottleneck convex edge, in-built filtration medium and to cover on bottleneck with externally threaded crown cap and in precession aglet to bottleneck convex edge shape up and down altogether the gesture of pressure to reach the object to bottle sealing, in the side wall upper part of crown cap, one outlet nozzle is installed, the intake ducting being connected with air filter goes directly at the bottom of bottle through the circular hole of crown cap central authorities.This utility model compact construction, easy to use, be beneficial to the storing of liquid spawn.
Above-mentioned several patent all has the features such as structure is simple, application cost is low, practical, but is not all configured with thermostat, and bacterial classification causes at the easy temperature influence of culturing process that the strain cultivation cycle is different in size, strain quality is also unstable.
Summary of the invention
Object of the present invention be exactly overcome aforesaid device facility deficiency and brand-new a kind of logical oxygen and the liquid fungus seed device of automatic constant-temperature are provided.Specifically comprise: the structure of this device is mainly made up of temperature bath, culturing bottle, air pump; Wherein, temperature bath is equipped with clear water, and culturing bottle is placed in temperature bath; Temperature bath is made up of temperature control instrument, heating tube, temp probe, culturing bottle is made up of bottle stopper, inlet pipe, strainer, air pump is linked together by strainer, sebific duct and inlet pipe, input oxygen by air pump toward culturing bottle, under the stirring of oxygen, constantly metabolism and dissociation must get more and more the mycelium (ball) in the liquid spawn cultivated in bottle; Control heating tube by temperature control instrument to heat the water inside temperature bath and to keep water temperature must scope, in culturing bottle, liquid spawn be cultivated under the temperature environment of the best, spawn culture cycle short, quality high-quality.
The present invention is achieved through the following technical solutions:
A liquid fungus seed device for logical oxygen and automatic constant-temperature, is in technical scheme: the structure of this device is mainly made up of temperature bath (1), culturing bottle (2), air pump (3); Wherein, temperature bath (1) is equipped with clear water, and culturing bottle (2) is placed in temperature bath (1); Temperature bath (1) is made up of temperature control instrument (4), heating tube (5), temp probe (6), heating tube (5) and temp probe (6) are all be placed in temperature bath (1), and are all be connected to temperature control instrument (4); Culturing bottle (2) is made up of bottle stopper (7), inlet pipe (8), strainer (9), inlet pipe (8) and strainer (9) are all penetrate and be placed in bottle stopper (7), and bottle stopper (7) is placed in the bottleneck of culturing bottle (2); Air pump (3) is linked together with inlet pipe (8) by strainer (10) and sebific duct (11); Strainer (9) the inside is placed with cotton (12), and strainer (10) the inside is placed with cotton (12) and gac (13).
Described temperature control instrument (4) and the relation of heating tube (5) and effect are: arrange certain temperature parameter by giving temperature control instrument (4), when the water temperature that the temp probe (6) being placed in temperature bath (1) detects temperature bath (1) the inside is lower than temperature parameter set by temperature control instrument (4), temperature control instrument (4) then starts heating tube (5), heating tube (5) feeds water heating immediately, when the water temperature of temperature bath (1) the inside conforms to the temperature parameter set by temperature control instrument (4), temperature control instrument (4) is then closed heating tube (5) and is stopped heating, thus reach the object keeping the water temperature inside temperature bath (1) in certain limit.
Described culturing bottle (2) its shape is cylinder shape, can bear and sterilize in high temperature, high pressure vessel;
Described air pump (3) is toward culturing bottle (2) input oxygen, mycelium (ball) in the liquid spawn cultivated in bottle continuous metabolism and dissociation under the stirring of oxygen must get more and more, and finally turn out the liquid spawn of high-quality.
Described strainer (9) and strainer (10) can bear high temperature, autoclave sterilization.
Described gac (13) is particulate state.
Described can bear high temperature, autoclave sterilization for the sebific duct (11) connecting air pump (3), strainer (10) and inlet pipe (8).
Compared with prior art, the invention has the beneficial effects as follows:
1, structure of the present invention is simple, but design science is reasonable, and not only cost is low, easy to use, and the liquid spawn production facilities and equipments that big-and-middle-sized fermentation tube, shaking table etc. can be replaced completely traditional produce liquid spawn.
2, when the present invention applies, control heating tube by temperature control instrument to heat the water inside temperature bath and to keep water temperature must scope, culturing bottle is placed in temperature bath, makes the liquid spawn inside bottle be cultivate under the temperature environment of the best, spawn culture cycle short, quality high-quality.
3, the glass sphere that application cost of the present invention is cheap is as strainer, and filtering system volume is little, and the strainer arranged at breather line is placed with granular gac, can adsorb the atmospheric moisture circulating in strainer, make the filter quality of strainer more superior.
4, little power consumption when the present invention applies, success ratio is high, good in economic efficiency, reduces production cost.
5, less investment of the present invention, instant effect, easy and simple to handle, is not only suitable for small-sized edible mushrooms factory and individual household with specialized lines of work use, but also can in rural households' wide popularization and application.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is structural representation of the present invention.
In FIG, 1 is temperature bath, and 2 is culturing bottles, 3 is air pumps, and 4 is temperature control instruments, and 5 is heating tubes, 6 is temp probes, and 7 is bottle stoppers, and 8 is inlet pipe, 9 is culturing bottle strainers, and 10 is line strainers, and 11 is sebific ducts, 12 is cottons, and 13 is gacs, and 14 is temp probe wires, 15 is heating tube supply leads, and 16 is supply leads of temperature control instrument, and 17 is supply leads of air pump.
Embodiment
Now the present invention will be described in detail by reference to the accompanying drawings:
The present invention is made up of temperature bath (1), culturing bottle (2), air pump (3).
In Fig. 1, 1 is temperature bath (1), 2 is culturing bottle (2), 3 is air pump (3), 4 is temperature control instrument (4), 5 is heating tube (5), 6 is temp probe (6), 7 is bottle stopper (7), 8 is inlet pipe (8), 9 is culturing bottle strainer (9), 10 is line strainer (10), 11 is sebific duct (11), 12 is cotton (12), 13 is gac (13), 14 is temp probe wire (14), 15 is heating tube supply lead (15), 16 is supply leads (16) of temperature control instrument, 17 is supply leads (17) of air pump.Wherein, temperature bath (1) is equipped with clear water, and culturing bottle (2) is placed in temperature bath (1); Temperature bath (1) is made up of temperature control instrument (4), heating tube (5), temp probe (6), heating tube (5) and temp probe (6) are all be placed in temperature bath (1), and are all be connected to temperature control instrument (4); Culturing bottle (2) is made up of bottle stopper (7), inlet pipe (8), strainer (9), inlet pipe (8) and strainer (9) are all penetrate and be placed in bottle stopper (7), and bottle stopper (7) is placed in the bottleneck of culturing bottle (2); Air pump (3) is linked together with inlet pipe (8) by strainer (10) and sebific duct (11); What strainer (9) the inside was placed is cotton (12), and what strainer (10) the inside was placed is cotton (12) and gac (13).
Relation and the effect of temperature control instrument (4) and heating tube (5) are: arrange certain temperature parameter by giving temperature control instrument (4), when the water temperature that the temp probe (6) being placed in temperature bath (1) detects temperature bath (1) the inside is lower than temperature parameter set by temperature control instrument (4), temperature control instrument (4) then starts heating tube (5), heating tube (5) feeds water heating immediately, when the water temperature of temperature bath (1) the inside conforms to the temperature parameter set by temperature control instrument (4), temperature control instrument (4) is then closed heating tube (5) and is stopped heating, thus reach the object keeping the water temperature inside temperature bath (1) in certain limit.
The shape of culturing bottle (2) is cylinder shape, can bear and sterilize in high temperature, high pressure vessel.
The effect of air pump is: by air pump toward culturing bottle (2) input oxygen, mycelium (ball) in the liquid spawn cultivated in bottle continuous metabolism and dissociation under the stirring of oxygen must get more and more, and finally turn out the liquid spawn of high-quality.
Strainer (9) and strainer (10) are all the ball-type Glass tubings that can bear high temperature, autoclave sterilization.Place granular gac (13) at strainer (10), the effect of gac (13) is: can adsorb the atmospheric moisture circulating in strainer, makes the filter quality of strainer more superior.
Sebific duct (11) for connecting air pump (3), strainer (10) and inlet pipe (8) can bear high temperature, autoclave sterilization.
Application method of the present invention and process: the liquid nutrient medium prepared is sub-packed in culturing bottle (2) that to carry out sterilization for subsequent use.Then strainer (10) and sebific duct (11) and inlet pipe (8) are coupled together, and and bottle stopper (7) combine and form vent filter system, filtering system is for subsequent use by sterilization equally.In an aseptic environment bacterial classification is accessed in culturing bottle (2), equally in an aseptic environment filtering system is fit in the bottleneck of culturing bottle (2), then culturing bottle (2) is positioned in temperature bath (1), setting temperature control instrument temperature parameter, the clear water starting temperature control instrument inner to temperature bath (1) carries out temperature control, sebific duct (11) and air pump (2) are coupled together simultaneously, booster air pump, toward culturing bottle (2) delivering oxygen, cultivate the bacterial classification of access, until cultivate, output is many, the liquid spawn of high-quality.
Claims (1)
1. a liquid fungus seed device for logical oxygen and automatic constant-temperature, is characterized in that: the structure of this device is mainly made up of temperature bath (1), culturing bottle (2), air pump (3); Wherein, temperature bath (1) is equipped with clear water, and culturing bottle (2) is placed in temperature bath (1); Temperature bath (1) is made up of temperature control instrument (4), heating tube (5), temp probe (6), heating tube (5) and temp probe (6) are all be placed in temperature bath (1), and are all be connected to temperature control instrument (4); Culturing bottle (2) is made up of bottle stopper (7), inlet pipe (8), strainer (9), inlet pipe (8) and strainer (9) are all penetrate and be placed in bottle stopper (7), and bottle stopper (7) is placed in the bottleneck of culturing bottle (2); Air pump (3) is linked together with inlet pipe (8) by strainer (10) and sebific duct (11); Strainer (9) the inside is placed with cotton (12), and strainer (10) the inside is placed with cotton (12) and gac (13);
Described temperature control instrument (4) and the relation of heating tube (5) and effect are: arrange certain temperature parameter by giving temperature control instrument (4), when the water temperature that the temp probe (6) being placed in temperature bath (1) detects temperature bath (1) the inside is lower than temperature parameter set by temperature control instrument (4), temperature control instrument (4) then starts heating tube (5), heating tube (5) feeds water heating immediately, when the water temperature of temperature bath (1) the inside conforms to the temperature parameter set by temperature control instrument (4), temperature control instrument (4) is then closed heating tube (5) and is stopped heating, thus reach the object keeping the water temperature inside temperature bath (1) in certain limit,
Described culturing bottle (2) its shape is cylinder shape, can bear and sterilize in high temperature, high pressure vessel;
Described air pump (3) is toward culturing bottle (2) input oxygen, mycelium (ball) in the liquid spawn cultivated in bottle continuous metabolism and dissociation under the stirring of oxygen must get more and more, and finally turn out the liquid spawn of high-quality;
Described strainer (9) and strainer (10) can bear high temperature, autoclave sterilization;
Described gac (13) is particulate state;
Described can bear high temperature, autoclave sterilization for the sebific duct (11) connecting air pump (3), strainer (10) and inlet pipe (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510505601.2A CN105199964A (en) | 2015-08-18 | 2015-08-18 | Oxygen-pumped and automatic constant-temperature liquid strain fermenting device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510505601.2A CN105199964A (en) | 2015-08-18 | 2015-08-18 | Oxygen-pumped and automatic constant-temperature liquid strain fermenting device |
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| CN105199964A true CN105199964A (en) | 2015-12-30 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2632092Y (en) * | 2003-06-17 | 2004-08-11 | 中国水产科学研究院黄海水产研究所 | Simple anaerobic fermenter |
| CN103125269A (en) * | 2012-12-25 | 2013-06-05 | 何寒 | Simple fermentation device for liquid spawn submerged cultivation |
| CN203057904U (en) * | 2012-12-25 | 2013-07-17 | 何寒 | Simple fermentation device for liquid strain submerged cultivation |
| KR20140144046A (en) * | 2013-06-10 | 2014-12-18 | (주)세포활성연구소 | Multi-purposerable Culture Machine for Mushroom Product |
-
2015
- 2015-08-18 CN CN201510505601.2A patent/CN105199964A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2632092Y (en) * | 2003-06-17 | 2004-08-11 | 中国水产科学研究院黄海水产研究所 | Simple anaerobic fermenter |
| CN103125269A (en) * | 2012-12-25 | 2013-06-05 | 何寒 | Simple fermentation device for liquid spawn submerged cultivation |
| CN203057904U (en) * | 2012-12-25 | 2013-07-17 | 何寒 | Simple fermentation device for liquid strain submerged cultivation |
| KR20140144046A (en) * | 2013-06-10 | 2014-12-18 | (주)세포활성연구소 | Multi-purposerable Culture Machine for Mushroom Product |
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Application publication date: 20151230 |