CN105194286B - Have effects that radiation protection and repair the external skin-care composition of skin barrier, essence and preparation method thereof - Google Patents
Have effects that radiation protection and repair the external skin-care composition of skin barrier, essence and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of external skin-care composition and preparation method thereof for having effects that radiation protection and repairing skin barrier, the composition is composed of the following weight percentages of raw materials: 2.0~5.0wt% of Chinese medical extract, 0.2~2.0wt% of ammonium acryloyl taurate/VP copolymer, EDTA0.01~0.08wt%, 1.0~5.0wt% of glycerol, 0.01~0.5wt% of preservative, water surplus；The Chinese medical extract is made of the bulk pharmaceutical chemicals that following weight parts match: honeysuckle 2~6, Radix Salviae Miltiorrhizae 1~4, Radix Codonopsis 1~4, Radix Astragali 1~3, Radix Ophiopogonis 1~2 and cactus 1~2.The present invention be verified by experiments it is non-stimulated to skin safe, the skin fibroblasts Proliferation Ability caused by electron radiation have good protective effect, can also effectively remove skin free radical, while having both bacteriostatic agent skin barrier and repairing effect.Raw material of the present invention obtain be easy, preparation method it is easy, it is green safe, be easy to be received by allergic human population, have a good application prospect and market prospects.
Description
Technical field
The present invention relates to a kind of external medicine compositions, specifically a kind of to have radiation protection and repair skin barrier function
The external medicine composition of effect and the skin care formulation being prepared by the skin care compositions.
Background technique
The mankind are a component parts of the ecosystem.People are survived by Environmental change condition, are made the life better；
Meanwhile the variation of environment also influences the clothing, food, lodging and transportion -- basic necessities of life of the mankind and the health of the mankind.There are a variety of in the external environment that we live
Influence the factor of skin health, the ultraviolet light as known to us, electric appliance radiation, haze, acid rain, Enviromental pollutants etc..In recent years
Come as the improvement of people's living standards, people's environmental pollution recognizing dangers are continuously improved.It is outer in order to protect the skin from
Boundary's infringement, research environment contamination hazard and its protection are becoming one of the hot spot of health care there research.
The skin care grade of infringement type is defendd to be pursued by consumer in existing market.Defend the research and development of infringement type skin care item many
Industry specialists are had an optimistic view of.Major product effect of consumer's concern has haze (preventing air contamination), radiation protection, anti-stimulation etc..
The defence principle of defence infringement type skin care item assists mainly still based on isolation with effect of releiving in the market.Protection approaches or list
One (such as sunscreen product) is unilateral (such as anti-irritant product), or lacks the successes achieved in research and support.Also it is invaded without comprehensive defence
Harmful product, consumer can only be in addition a using after using a product, increase consumer and use complicated journey
Degree.
In addition, the covering gradually popularized with mobile telephone base station with mobile phone is more and more intensive, people's moment is in hand
In the radiation field of machine.Although the photon energy of mobile phone is lower than minimum 10,000,000 times of energy ionizing radiation, far field can also be caused
The threat of radiation.And skin at this time is one of exposed area Maximum Contact time longest organ, and therefore, cell phone microwave radiation
Influence to skin molds structure also can not be ignored.Inventors have found that people are after long-time is using mobile phone, mobile phone radiation can be made
At the fibroblastic Proliferation Ability of skin, pathogenic microorganism increases on skin, to destroy skin barrier, skin is caused to decline
Always.Presently commercially available skin-protection product cannot effectively inhibit mobile phone and other electromagnetic radiation such damage caused by skin.Cause
This is by removing free radical, inhibition radiation cause fibroblast proliferation, inhibiting skin sex pheromone, maintenance skin barrier effect
Approach, the environmental pollution of resisting for reaching comprehensive is the emphasis of the invention studied to the damage of skin.
Summary of the invention
The primary purpose of the present invention is that proposing a kind of external-use skin care group for having effects that radiation protection and repairing skin barrier
Close object.
The facial treatment essence that another object of the present invention is to propose a kind of preparation method of above-mentioned skin care compositions and obtains
Element.
Invention thinking of the invention are as follows:
The present invention utilizes advanced biotechnology, in conjunction with the theory of traditional Chinese medical science of Chinese tradition, by honeysuckle, Radix Salviae Miltiorrhizae, Radix Codonopsis, Huang
Stilbene, Radix Ophiopogonis and cactus are effectively combined in together, form compound, and as active component, are prepared into skin care formulation,
Have effects that protection radiation causes fibroblast proliferation to inhibit, inhibits skin sex pheromone and maintenance skin barrier, so as to
Effectively prevention and improvement skin aging.The present invention is described as follows using bulk pharmaceutical chemicals:
Honeysuckle: it is cold in nature, it is sweet in flavor, enter lung, the heart, stomach meridian, has effects that clearing heat and detoxicating, anti-inflammatory, qi-restoratives treats wind, cure mainly swollen
Completely descend disease, warm disease heating, the diseases such as heat toxin large carbuncle and tumour.
Radix Salviae Miltiorrhizae: it is bitter in taste, it is slightly cold.Converge to heart and liver channels.There is the effect of stasis-dispelling and pain-killing, blood circulation, relieving restlessness and restlessness.For the moon
Through uncomfortable, amenorrhea and algomenorrhea, a lump in the abdomen causing distension and pain, chest and abdomen shouting pain, hot numbness pain, sore and ulcer, swelling and pain, upset and sleepless；Hepatosplenomegaly, angina pectoris.
Radix Codonopsis: it is sweet in flavor, it is mild-natured.There is tonifying middle-Jiao and Qi, quench the thirst, strengthening spleen and tonifying lung, nourishes blood and promotes the production of body fluid effect.For spleen-lung Qi deficiency, food
Few burnout, false asthma cough, insufficiency of vital energy and blood, sallow complexion, shortness of breath and palpitation, injury thirst, Heat Diabetes.
Radix Astragali: nature and flavor: sweet, tepor.Return lung, spleen, liver and kidney channel.To exterior deficiency spontaneous perspiration, yang-energy weakness, sore ulceration and lung qi
Asthenic symptoms have certain effect.
Radix Ophiopogonis: nature and flavor are sweet, and slight bitter is slightly cold.Return heart, lung, stomach meridian.Function with the major functions: nourishing Yin and promoting production of body fluid, moistening lung and clearing away the heart-fire.For
Dryness of the lung dry cough, cough for deficiency of Yin consumptive disease, larynx numbness pharyngalgia, injury thirst, Heat Diabetes, vexed insomnia, dry constipation of intestines.
Cactus: nature and flavor slight bitter, it is cool.Return heart, lung, stomach three pass through.With clearing heat and detoxicating, relaxing tendons and activating collaterals, eliminating stasis to subdue swelling solves intestines
The effect of poison, cooling blood and relieving pain, ease constipation hemostasis, benefiting stomach and stopping pain and antibechic.
To achieve the purpose of the present invention, the present invention uses following specific technical solution:
A kind of external skin-care composition for having effects that radiation protection and repairing skin barrier, wherein the composition is under
State the raw material composition of weight percent:
The Chinese medical extract is made of the bulk pharmaceutical chemicals that following weight parts match:
Honeysuckle 2~6, Radix Salviae Miltiorrhizae 1~4, Radix Codonopsis 1~4, Radix Astragali 1~3, Radix Ophiopogonis 1~2 and cactus 1~2.
The Chinese medical extract the preparation method comprises the following steps:
(1) by above-mentioned raw materials medicinal powder it is broken after weigh by the weight, mix；
(2) percent by volume is the extraction of 60%~90% ethyl alcohol, and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:8~1:
20,70 DEG C~90 DEG C are extracted 1~4 hour；
(3) extracting solution that step (2) obtains is cooled to 20 DEG C~30 DEG C, it is laggard to filter out the dregs of a decoction for the filtering of 200~400 mesh
Row vacuum filtration, collects filtrate；
(4) filtrate rotary evaporation whole ethyl alcohol is obtained into concentrate, 1,3-BDO is added in concentrate, additional amount is step
Suddenly 60%~90% of amount of filtrate percent by volume obtained by (3)；It is miscible uniformly, filtering, take clear liquid to get.
The percent by volume of ethyl alcohol is 70% in the step (2), and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:15；
Extraction time is 3 hours.
Vacuum filtration condition is that two layers of filter paper is spread in Buchner funnel in the step (3), filter paper centre folder 0.3~
0.6cm diatomite is filtered by vacuum.
A kind of preparation method of above-mentioned skin care compositions, wherein the method comprises the following steps:
(1) EDTA glycerol is add to deionized water, is stirred after being heated to 80 DEG C~90 DEG C to being completely dissolved；
(2) ammonium acryloyl taurate/VP copolymer is added, stirring makes it completely dissolved；
(3) 40 DEG C are cooled to hereinafter, preservative and the Chinese medical extract is added, stir evenly to get.
Another kind have effects that radiation protection and reparation skin barrier external skin-care composition, wherein the composition by
The raw material of following weight percentage forms:
The Chinese medical extract is made of the bulk pharmaceutical chemicals that following weight parts match:
Honeysuckle 2~6, Radix Salviae Miltiorrhizae 1~4, Radix Codonopsis 1~4, Radix Astragali 1~3, Radix Ophiopogonis 1~2 and cactus 1~2.
The preparation method of the Chinese medical extract is the same as above-mentioned.
A kind of preparation method of above-mentioned skin care compositions, wherein the method comprises the following steps:
(1) first water-soluble jojoba oil is dissolved by heating with water；
(2) EDTA, oat polypeptide, Sodium Hyaluronate, glycerol, allantoin, aloe powder, stimulation inhibiting factor are added to
In ionized water, stirred after being heated to 80 DEG C~90 DEG C to being completely dissolved；
(3) ammonium acryloyl taurate/VP copolymer is added, stirring makes it completely dissolved；
(4) 40 DEG C are cooled to hereinafter, preservative and the Chinese medical extract is added, stir evenly to get.
Obtained skin care formulation is facial treatment repair.It is conventional using Chinese medical extract of the present invention and skin care field
Other dosage forms, such as cream, toner, spray can also be prepared in method and auxiliary material.The system of the traditional chinese medicine composition of the invention
Preparation Method is not limited to this specification record, and field of Chinese medicines customary preparation methods can play described effect.Side of the present invention
Method is best.
Preparation of the present invention is recommended to use method are as follows: after daily face cleaning, Essence is applied on the skin, is gently pressed
It rubs, is slowly absorbed to essence.
Beneficial outcomes of the invention:
The present invention is verified by experiments, electron radiation caused by skin fibroblasts proliferation suppression non-stimulated to skin safe
Fixture has good protective effect, can also effectively remove skin free radical, while having both bacteriostatic agent and skin barrier reparation
Effect.Easy, preparation method simplicity that raw material of the invention obtain, it is green safe, it is easy to be received by allergic human population, has fine
Application prospect and market prospects.
Detailed description of the invention
Fig. 1 is the DPPH clearance rate figure of Chinese medicine composition A, B, C；
Fig. 2 is the percentage of water loss percentage versus time curve of skin surface.
Specific embodiment
Medicinal material used in the present embodiment can be commercially available from China Medicament Group Corp., meet People's Republic of China's medicine
2005 editions standards of allusion quotation.Other raw materials can be obtained by commercially available purchase, and the present invention is shown in Table 1 using the source of raw material, institute of the present invention
Instrument title and producer are shown in Table 2.
Table 1
Table 2
| Title | Model | Producer |
| Thermostat water bath | HH·S1-M | Beijing Chang'an scientific instrument factory |
| High-speed multifunctional pulverizer | JP-400B-8 | Yongkang City nine grades of rank in the feudal regimes Trade Co., Ltd. |
| Intelligent temperature control heating stirrer | SZCL | Yu Hua Instrument Ltd., Gongyi City |
| Rotary evaporator | RE-2000 | Shanghai Yarong Biochemical Instrument Plant |
| Circulating water type vacuum pump | SHB-Ⅲ | Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd. |
The preparation of 1 the traditional chinese medicine composition of the invention of embodiment
(1) it weighs, mixes by the weight after being crushed according to following raw material medicaments；
Honeysuckle 40g, Radix Salviae Miltiorrhizae 20g, Radix Codonopsis 20g, Radix Astragali 20g, Radix Ophiopogonis 10g and cactus 10g.
(2) percent by volume is the extraction of 70% ethyl alcohol, and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:15, is placed in electricity
In hot thermostat water bath, 80 DEG C are extracted 3 hours；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, standing is cooled to room temperature (20 DEG C), 200 mesh
Coarse filtration filters out the dregs of a decoction, then is filtered by vacuum, and filters condition: two layers of filter paper, folder among filter paper are spread in Buchner funnel
0.5cm diatomite vacuum filtration, collects filtrate；
(4) rotary evaporation whole ethyl alcohol obtains concentrate under the conditions of 0.1,43 DEG C of vacuum degree；1,3- fourth is added in concentrate
Glycol, additional amount are 70% of amount of filtrate percent by volume obtained by step (3), miscible uniform.Vacuum filtration filters conditional synchronization
Suddenly (3), take clear liquid to get.
The preparation of 2 the traditional chinese medicine composition of the invention of embodiment
(1) it weighs, mixes by the weight after being crushed according to following raw material medicaments；
Honeysuckle 60g, Radix Salviae Miltiorrhizae 10g, Radix Codonopsis 10g, Radix Astragali 10g, Radix Ophiopogonis 20g and cactus 10g.
(2) percent by volume is the extraction of 90% ethyl alcohol, and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:8, is placed in electricity
In hot thermostat water bath, 70 DEG C are extracted 4 hours；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, standing is cooled to room temperature, the thick mistake of 200 mesh
Filter, filters out the dregs of a decoction, obtains filtrate；The filtrate is filtered by vacuum again, condition is filtered: spreading two layers of filter paper in Buchner funnel, is filtered
Folder 0.3cm diatomite vacuum filtration, collects filtrate among paper；
(4) rotary evaporation whole ethyl alcohol obtains concentrate under the conditions of 0.1,43 DEG C of vacuum degree；1,3- fourth is added in concentrate
Glycol, additional amount are 90% of amount of filtrate percent by volume obtained by step (3), miscible uniform.Vacuum filtration filters conditional synchronization
Suddenly (3), take clear liquid to get.
The preparation of 3 the traditional chinese medicine composition of the invention of embodiment
(1) it weighs, mixes by the weight after being crushed according to following raw material medicaments；
Honeysuckle 20g, Radix Salviae Miltiorrhizae 40g, Radix Codonopsis 40g, Radix Astragali 30g, Radix Ophiopogonis 10g and cactus 20g.
(2) percent by volume is the extraction of 60% ethyl alcohol, and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:20, is placed in electricity
In hot thermostat water bath, 90 DEG C are extracted 1 hour；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, standing is cooled to room temperature, the thick mistake of 100 mesh
Filter, filters out the dregs of a decoction, obtains filtrate；The filtrate is filtered by vacuum again, condition is filtered: spreading two layers of filter paper in Buchner funnel, is filtered
Folder 0.6cm diatomite vacuum filtration, collects filtrate among paper；
(4) rotary evaporation whole ethyl alcohol obtains concentrate under the conditions of 0.1,43 DEG C of vacuum degree；1,3- fourth is added in concentrate
Glycol, additional amount are 60% of amount of filtrate percent by volume obtained by step (3), miscible uniform.Vacuum filtration filters conditional synchronization
Suddenly (3), take clear liquid to get.
The preparation of the essence of the present invention of embodiment 4
Raw material and proportion (mass percent), are shown in Table 3.
Table 3
| Material name | Dosage (wt%) |
| Embodiment 1 prepares Chinese medical extract | 5.0 |
| AVC | 2.0 |
| EDTA | 0.01 |
| Glycerol | 5.0 |
| Preservative | 0.10 |
| Deionized water | Surplus |
Preparation method:
(1) EDTA glycerol is add to deionized water, is stirred after being heated to 80 DEG C to being completely dissolved；
(2) AVC is added, stirring makes it completely dissolved；
(3) 40 DEG C are cooled to hereinafter, preservative and the Chinese medicine extract is added, stir evenly to get.
The preparation of the essence of the present invention of embodiment 5
Raw material and proportion (mass percent), are shown in Table 4.
Table 4
| Material name | Dosage (wt%) |
| Embodiment 2 prepares Chinese medical extract | 2.0 |
| AVC | 1.0 |
| EDTA | 0.08 |
| Glycerol | 1.0 |
| Preservative | 0.01 |
| Deionized water | Surplus |
Preparation method:
(1) EDTA glycerol is add to deionized water, is stirred after being heated to 90 DEG C to being completely dissolved；
(2) AVC is added, stirring makes it completely dissolved；
(3) 40 DEG C are cooled to hereinafter, preservative and the Chinese medicine extract is added, stir evenly to get.
The preparation of the essence of the present invention of embodiment 6
Raw material and proportion (mass percent), are shown in Table 5.
Table 5
| Material name | Dosage (wt%) |
| Embodiment 3 prepares Chinese medical extract | 3.0 |
| AVC | 0.2 |
| EDTA | 0.05 |
| Glycerol | 3.0 |
| Preservative | 0.5 |
| Deionized water | Surplus |
Preparation method:
(1) EDTA glycerol is add to deionized water, is stirred after being heated to 85 DEG C to being completely dissolved；
(2) AVC is added, stirring makes it completely dissolved；
(3) 40 DEG C are cooled to hereinafter, preservative and the Chinese medicine extract is added, stir evenly to get.
The preparation of the essence of the present invention of embodiment 7
Raw material and proportion (mass percent), are shown in Table 6.
Table 6
| Material name | Dosage (wt%) |
| Embodiment 1 prepares Chinese medical extract | 5.0 |
| AVC | 2.0 |
| EDTA | 0.01 |
| Oat polypeptide | 7.0 |
| Water-soluble jojoba oil | 1.0 |
| Sodium Hyaluronate | 0.01 |
| Glycerol | 5.0 |
| Allantoin | 0.1 |
| Aloe powder | 0.5 |
| Stimulate inhibiting factor | 5.0 |
| Preservative | 0.10 |
| Deionized water | Surplus |
Preparation method:
(1) first water-soluble jojoba oil is dissolved by heating with deionized water；
(2) EDTA, oat polypeptide, Sodium Hyaluronate, glycerol, allantoin, aloe powder, stimulation inhibiting factor are added to
In ionized water, stirring and dissolving after being heated to 80 DEG C.
(3) AVC is added, stirring makes it completely dissolved.
(4) continue to be cooled to 40 DEG C hereinafter, preservative and Chinese medical extract is added, stir evenly to get.
The preparation of the essence of the present invention of embodiment 8
Raw material and proportion (mass percent), are shown in Table 7.
Table 7
| Material name | Dosage (wt%) |
| Embodiment 2 prepares Chinese medical extract | 2.0 |
| AVC | 0.2 |
| EDTA | 0.08 |
| Oat polypeptide | 1.0 |
| Water-soluble jojoba oil | 0.2 |
| Sodium Hyaluronate | 0.05 |
| Glycerol | 1.0 |
| Allantoin | 0.2 |
| Aloe powder | 0.1 |
| Stimulate inhibiting factor | 1.0 |
| Preservative | 0.01 |
| Deionized water | Surplus |
Preparation method:
(1) first water-soluble jojoba oil is dissolved by heating with deionized water；
(2) EDTA, oat polypeptide, Sodium Hyaluronate, glycerol, allantoin, aloe powder, stimulation inhibiting factor are added to
In ionized water, stirred after being heated to 80 DEG C to being completely dissolved；
(3) AVC is added, stirring makes it completely dissolved；
(4) continue to be cooled to 40 DEG C hereinafter, preservative and Chinese medical extract is added, stir evenly to get.
The preparation of the essence of the present invention of embodiment 9
Raw material and proportion (mass percent), are shown in Table 8.
Table 8
| Material name | Dosage (wt%) |
| Embodiment 3 prepares Chinese medical extract | 4.0 |
| AVC | 1.0 |
| EDTA | 0.03 |
| Oat polypeptide | 5.0 |
| Water-soluble jojoba oil | 0.5 |
| Sodium Hyaluronate | 0.03 |
| Glycerol | 3.0 |
| Allantoin | 0.15 |
| Aloe powder | 0.3 |
| Stimulate inhibiting factor | 3.0 |
| Preservative | 0.5 |
| Deionized water | Surplus |
Preparation method:
(1) first water-soluble jojoba oil is dissolved by heating with deionized water；
(2) EDTA, oat polypeptide, Sodium Hyaluronate, glycerol, allantoin, aloe powder, stimulation inhibiting factor are added to
In ionized water, stirred after being heated to 80 DEG C to being completely dissolved；
(3) AVC is added, stirring makes it completely dissolved；
(4) continue to be cooled to 40 DEG C hereinafter, preservative and Chinese medical extract is added, stir evenly to get.
Efficacy experiments of the present invention
One, embodiment 1, embodiment 2, the evaluation of the anti-radiation effect of embodiment 3 gained Chinese medical extract A, B, C
(1) research purpose
By observation Chinese medical extract A, B, C (being prepared respectively by embodiment 1, embodiment 2 and embodiment 3) to mobile phone
The influence that Mouse Skin Fibroblasts strain 3T6 is grown after radiation, evaluation Chinese medicine composition A, B, C inhibit cell to mobile phone radiation
The protective effect of growth.
(2) experimental material
By test product: embodiment 1, embodiment 2, embodiment 3 gained Chinese medical extract A, B, C
Cell strain: Mouse Skin Fibroblasts strain 3T6 is purchased from Cell Bank of Chinese Academy of Sciences, makes after this laboratory passage
With.
Reagent: FBS (lot number: 1428479), 0.25%Trypsin-EDTA (lot number: 1213981) be purchased from GIBCO company.
Modified form RPMI-1640 type culture medium (lot number: NXL0745) is purchased from HYCLONE company.DMSO (lot number: 20121102) is purchased from
Sinopharm Chemical Reagent Co., Ltd..Cell proliferation-toxicity detection-CCK-8 kit is Japanese colleague's DOJINDO chemistry
Research institute's product.
Laboratory apparatus: X71 type inverted microscope is Olympus Products.HH CP-01W type carbon dioxide cell culture
Case is Medical Equipment Plant, Shanghai Boxun Industrial Co., Ltd. product.The single single side clean work station of SW-CJ-IFD type is that Suzhou is net
Change equipment Co., Ltd product.RT-6000 type microplate reader, Shenzhen thunder Du's Products.1050 type of Nokia's mobile phone, frequency range are
GSM900MHz。
(3) experimental method
Experiment is divided into blank control group, radiation control group, drug various concentration group, every group of 8 multiple holes.It is thin to collect logarithmic phase
Born of the same parents count, by 1 × 104/ ml concentration is inoculated into 96 orifice plates, every 200 μ l of hole.37 DEG C are set, 5%CO224 are cultivated in cell incubator
Hour, keep cell adherent.Different 100 μ l of Chinese medical extract are added in medication group, and 100 μ l are added in blank control group and radiation control group
Serum free medium continues to cultivate.After 24 hours, cell plates are placed in CO2Incubator exoadaptation 1 hour, remove blank control group
Extracellularly, other cell plates are placed on above mobile phone, mobile phone keeps talking state, and blank control group cell plates are in another room
It is placed in above mobile phone, mobile phone remains off.After 6 hours using CCK method detect cell activity, and calculate proliferation inhibition rate and
Proliferation Ability protective rate.
(4) experimental result
The results show that using after mobile phone radiation 6 hours, skin fibroblasts proliferation is suppressed, so that absorbance drops
Low, radiation cellular control unit OD value relatively has significant difference (P < 0.01) compared with blank control group, and proliferation inhibition rate reaches
16.89%；Chinese medical extract A, B, C dosage are in 1000 μ gml-1Shi Jun has apparent protect to cell inhibitory effect after radiation
Shield effect can effectively reduce radiation to fibroblastic inhibiting effect, so that absorbance rises, with radiation control group ratio
Relatively there is significant difference (P < 0.05).9 are shown in Table, Chinese medical extract A's inhibits protective rate reachable fibroblast proliferation
11.86%.
Table 9 Chinese medical extract A, B, C are to cell Proliferation/toxicity influence after radiation
| Group | Concentration μ gml-1 | Absorbance | Proliferation inhibition rate (%) | Proliferation Ability protective rate (%) |
| Chinese medical extract A | 1000 | 1.18±0.09* | -- | 11.86 |
| Chinese medical extract B | 1000 | 1.15±0.07* | -- | 9.57 |
| Chinese medical extract C | 1000 | 1.13±0.05* | -- | 7.96 |
| Radiate control group | -- | 1.04±0.09## | 16.89 | -- |
| Blank control group | -- | 1.26±0.05 | -- | -- |
Two, embodiment 1, embodiment 2,3 gained Chinese medical extract A, B, C of embodiment are to the protection effect for radiating indirectly-acting
Radiating the effect to bioactive substance, there are two approach: first is that directly acting on large biological molecule such as DNA, passing through
Ionization and excitation bring it about the fracture of chemical bond, cause the change of molecular structure and the forfeiture of bioactivity, and this effect claims
Directly to act on；Second is that ray and hydrone interact, activation products, the latter such as generates free radicals and act on biology point
Son, causes its damage, and such effect is known as indirectly-acting.In vivo, due to a large amount of presence of hydrone, indirectly-acting tool
There is prior meaning.Therefore, for radiation indirectly-acting, can choose remove free radical effect come evaluate Chinese medical extract A,
B, the protection effect of C to radiation indirectly-acting.
Chinese medical extract obtained by Examples 1 to 3 and deionized water are mixed with the mass ratio of 3:97, obtain Chinese medical extract
Aqueous solution；Using the aqueous solution of the Chinese medical extract as test group；Wherein, 1 gained Chinese medical extract A counter sample of embodiment
1；2 gained Chinese medical extract B counter sample 2 of embodiment；3 gained Chinese medical extract C counter sample 3 of embodiment；It will be in test group
Chinese medical extract replace with vitamin C derivatives, obtained aqueous solution is as positive control.
Measurement result such as Fig. 1.The experimental results showed that quality hundred of the Chinese medical extract obtained by each embodiment in its aqueous solution
When point content is 3.0%, have obviously to DPPH Scavenging activity, having reduces hydroxy radical, alkane free radical or oxidation certainly
By base effective concentration and interrupt the effect of lipid peroxidation chain reaction, thus the work with good delaying skin radiative aging
With.
Three, the bacteria resistance function evaluation of embodiment 1, embodiment 2, embodiment 3 gained Chinese medical extract A, B, C
The air pollutions such as haze enable the content of microorganisms in environment increase, wherein common Escherichia coli, golden yellow grape
Phenomena such as coccus, propionibacterium acnes contact skin easily cause skin acne, sensitivity.
Chinese medical extract A, B, C obtained by Examples 1 to 3 are subjected to Escherichia coli, staphylococcus aureus, acne propionic acid bar
Bacterium inhibits efficacy assessments.
Odontothrips loti: configuring corresponding culture medium according to different strains testeds, and 121 DEG C of high pressure steam sterilization, 20min.Production
Solid medium tablets, each culture dish about pour into 20mL culture medium, after culture medium solidification, take 100 μ L bacterium solutions with liquid-transfering gun
(bacteria suspension for having been subjected to Maxwell turbidimetry for Determination concentration) instills in culture dish, and using spread plate, bacteria suspension is uniformly applied
It is distributed on solid medium.Oxford cup (the circle of internal diameter 6mm, outer diameter 8mm, high 10mm is directly vertically put in media surface
Tubule), it gently pressurizes, it is made to contact tight with culture medium.Each culture dish places three Oxford cups.Add in each Oxford cup
Enter 200 μ L samples to be tested, the culture dish of bacterium is gently placed in or so 37 DEG C of 16 hours of culture in bacteriological incubator.
Wherein, 1 gained Chinese medical extract A counter sample 1 of embodiment；2 gained Chinese medical extract B counter sample 2 of embodiment；
3 gained Chinese medical extract C counter sample 3 of embodiment；Positive control is 0.2%MTI (methylisothiazolinone/iodine propinyl fourth
Methyl carbamic ester).MTI is common preservative in cosmetics, it is proposed that dosage is 0.05%~0.02%.It is equal to bacterium, fungi
There is excellent antibacterial activity.Measurement result such as table 10.
The evaluation of 10 Chinese medical extract A, B, C fungistatic effect of table
| Antibacterial circle diameter (mm) | Escherichia coli | Staphylococcus aureus | Propionibacterium acnes |
| Sample 1 | 24.95±0.22 | 14.71±0.18 | 16.94±0.42 |
| Sample 2 | 20.36±0.46 | 16.09±0.17 | 14.27±0.11 |
| Sample 3 | 19.90±0.57 | 13.04±0.39 | 14.65±0.78 |
| Positive control | 21.23±0.64 | 17.92±0.37 | 20.90±0.98 |
Note: antibacterial circle diameter >=20mm is that extremely sensitive " +++ " has been judged to bacteriostasis, and the bigger fungistatic effect of inhibition zone is more
It is good；15mm≤bacteriostatic diameter < 20mm is Gao Min " ++ "；10mm≤bacteriostatic diameter < 15mm be in quick "+"；Bacteriostatic diameter < 10mm is
In vain, it is judged to no bacteriostasis.With reference to susceptibility test methods [J] animal and veterinary scientific and technological information of Chen Yu duckweed antibacterials,
2007, (5): 88-89.
From experimental result as can be seen that sample 1, sample 2, sample 3 all have good fungistatic effect, can be effectively reduced
Adverse effect of the microorganism to skin in environment.
Four, embodiment 7, embodiment 8, the evaluation of the anti-radiation effect of 9 gained preparation of embodiment
7 gained preparation of embodiment is corresponded into essence 1；8 gained preparation of embodiment corresponds to essence 2；It is made obtained by embodiment 9
Agent corresponds to essence 3.
Separately using table 11 as negative control.Negative control essence used the preparation method is as follows:
It is formulated according to obtained by table 11, is first dissolved by heating water-soluble jojoba oil with deionized water.EDTA, oat is more
Peptide, Sodium Hyaluronate, glycerol, allantoin, aloe powder, stimulation inhibiting factor are add to deionized water after being heated to 80 DEG C and stir
Dissolution；AVC is added, stirring makes it completely dissolved.40 DEG C are cooled to hereinafter, addition preservative, stirs evenly.
11 negative control essence formula of table
| Material name | Dosage (wt%) |
| AVC | 2.0 |
| EDTA | 0.01 |
| Oat polypeptide | 7.0 |
| Water-soluble jojoba oil | 1.0 |
| Sodium Hyaluronate | 1.0 |
| Glycerol | 5.0 |
| Allantoin | 0.1 |
| Aloe powder | 0.5 |
| Stimulate inhibiting factor | 5.0 |
| Deionized water | Surplus |
| Preservative | 0.10 |
Kunming kind hairless mouse, 60, half male and half female, 18~22g of weight is had by Beijing dimension tonneau China experimental animal technology
Limit company provides.
Experimental method:
60 mouse are randomly divided into 6 groups, it may be assumed that and 2. 1. Normal group radiates control group, 3. 1 group of sample, 4. 2 groups of sample,
5. 3 groups of sample, 6. negative control group.Every group 10, by gender and group sub-cage rearing, under identical environment, control temperature dimension
20~25 DEG C are held, 12h illumination, 12h is dark, and conventional feeding drinking-water, no undesirable element influences, and raises one week adaptation laboratory environment
After start to be tested.
Mobile phone is placed in the cage exclusively for this experimental design (diameter 45cm, high 11cm).Using Nokia's hand
1050 type (frequency range GSM900MHz) of machine carries out radiation test as radiation source.
60 mouse are randomly divided into 6 groups (every group 10), one group is not smeared sample, and mobile phone remains turned-off shape during experiment
State, as Normal group.One group is not smeared sample, and mobile phone keeps opening state during experiment, and when daily 10,14,
When 16, in an ON state, 15min is connected every time, as radiation control group.Other four groups are used as experimental group, during experiment, hand
Machine keeps opening state, and when daily 10,14,16 when, in an ON state, connect 15min every time.Sample is smeared respectively
1, sample 2, sample 3, negative control essence sample.Sample is uniformly embrocated in back of mice respectively, is every time 0.5mL,
Once a day, mouse is placed in the cage of radiation source after smearing uniformly, each 45min continues 96 days once a day.
96th day, back of mice instrument is lost hair or feathers, area is 2cm × 3cm.After disconnected neck puts to death whole mouse, remove immediately
Full thickness skin (being free of subcutaneous fat) at the depilation of back, it is to be measured to be frozen in preservation in -70 DEG C of refrigerators.Using histopathologic slide's system
Chip technology, HE dyeing, is changed with 40 times of om observation dermis, and with biochemical analysis method measurement SOD activity, MDA content.
SOD can remove super oxygen cation (i.e. oxygen radical), protect cells from damage.SOD vigor height can between it is reversed
The ability of film projector body scavenging activated oxygen.Free radical attacks the unsaturated fatty acid in film rouge, brings it about oxidation, lipid peroxidation
The decomposition product MDA of matter will be increased, and by measuring internal MDA content, can reflect damage of the interior free yl to body indirectly
Degree.
All data pass through SPSS19.0 software and are handled, and each group experiment parameter is indicated with .x ± s.
SOD, MDA content (x ± s) (n=10) in 12 each group hairless mouse skin tissue of table
SOD, MDA content are shown in Table 12 in mouse skin.As the result is shown: embodiment 7 (counter sample 1), embodiment 8 are (corresponding
Sample 2) and embodiment 9 (counter sample 3) all have anti-radiation effect.And the protection effect of embodiment 7 is most significant.Inventor's benefit
Above-mentioned experiment is repeated with 4,5,6 gained essence of embodiment, all has anti-radiation effect as the result is shown.
Five, the maintenance barrier evaluation of 7 gained preparation of embodiment
1. cuticula moisture loss is tested
Test sample is essence, prepares and obtains as follows:
7 gained preparation of embodiment is corresponded into essence 1.
Separately using 11 essence of table as blank control.Blank control essence preparation method used and above-mentioned anti-radiation effect
Negative control preparation method in evaluation is identical.
Test before through the trained health volunteer 30 of professional (each 15 of men and women), the age at 20~30 years old, institute
Have subject without skin or systemic disease history, recipient site is without exception, and tested period do not smear it is any unrelated with experiment
Drug or cosmetics.
Test site room temperature (25 ± 1) DEG C, relative humidity (40 ± 5) %, subject should keep body to stablize before testing
State.After wiping subject's both arms using 35 DEG C or so of clear water, start to test after testing under environment the 30min that sits quietly.
At subject's inboard arm is away from palm base portion 5cm, mark tested region (4cm × 4cm), same arm can be simultaneously
Mark multiple regions (interval 1cm), the equal random distribution of test sample.The blank value of each test zone is first measured, then test specimens
By (2.0 ± 0.1) mg/cm2Dosage carry out single application, after coating use Multi-functional skin moisture tester and moisture of skin
Lost instrument measures the moisture loss magnitude in the tested region 0.5h, 1h, 2h and 4h and blank control region respectively.
Subject is smeared 5 data measured in same sample region and is averaged by test, using SPSS statistical software
Carry out t check analysis.
Experimental result
Moisture of skin, which scatters and disappears, to be changed
Moisture of skin windage (Transepidermal water loss, TEWL) reflects in test period, test block
Domain moisture loss changes with time rule, it can be worth smaller, moisture with the skin barrier maintenance function of characterization test sample
It scatters and disappears fewer, skin repair ability is stronger；Conversely, skin repair ability is weaker.
Percentage of water loss (%)=(sample sets water loss amount-starting moisture windage)/starting moisture windage × 100
Moisture loss result is shown in Fig. 2.As the result is shown: in 4h, embodiment 7 (counter sample 1) is imitated with skin repair ability
Fruit.The essence that inventor is prepared with embodiment 4,5,6,8,9 repeats above-mentioned experimental result and is same as above.And the maintenance effect of embodiment 7
Fruit is most significant.
Claims (10)
1. a kind of external skin-care composition for having effects that radiation protection and repairing skin barrier, it is characterised in that the external-use skin care
Composition is made of the raw material of following weight percentage:
The Chinese medical extract is made of the bulk pharmaceutical chemicals that following weight parts match:
Honeysuckle 2~6, Radix Salviae Miltiorrhizae 1~4, Radix Codonopsis 1~4, Radix Astragali 1~3, Radix Ophiopogonis 1~2 and cactus 1~2.
2. external skin-care composition as described in claim 1, it is characterised in that the Chinese medical extract the preparation method comprises the following steps:
(1) it weighs, mixes by the weight after crushing bulk pharmaceutical chemicals described in claim 1；
(2) percent by volume is the extraction of 60%~90% ethyl alcohol, and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:8~1:20,
70 DEG C~90 DEG C are extracted 1~4 hour；
(3) extracting solution that step (2) obtains is cooled to 20 DEG C~30 DEG C, the filtering of 200~400 mesh carries out very after filtering out the dregs of a decoction
Empty pump filter, collects filtrate；
(4) filtrate rotary evaporation whole ethyl alcohol is obtained into concentrate, 1,3-BDO is added in concentrate, additional amount is step
(3) the 60%~90% of gained amount of filtrate percent by volume；It is miscible uniformly, filter to take clear liquid to get.
3. external skin-care composition as claimed in claim 2, which is characterized in that the volume basis of ethyl alcohol in the step (2)
Than being 70%, bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:15；Extraction time is 3 hours.
4. external skin-care composition as claimed in claim 2, which is characterized in that vacuum filtration condition is in the step (3)
Two layers of filter paper is spread in Buchner funnel, folder 0.3~0.6cm diatomite is filtered by vacuum among filter paper.
5. the preparation method of external skin-care composition described in a kind of any one of Claims 1-4, it is characterised in that the system
The step of Preparation Method, is as follows:
(1) EDTA glycerol is added to the water, is stirred after being heated to 80 DEG C~90 DEG C to being completely dissolved；
(2) ammonium acryloyl taurate/VP copolymer is added, stirring makes it completely dissolved；
(3) 40 DEG C are cooled to hereinafter, addition preservative and the Chinese medical extract, stir evenly to obtain the final product.
6. a kind of external skin-care composition for having effects that radiation protection and repairing skin barrier, it is characterised in that the external-use skin care
Composition is made of the raw material of following weight percentage:
The Chinese medical extract is made of the bulk pharmaceutical chemicals that following weight parts match:
Honeysuckle 2~6, Radix Salviae Miltiorrhizae 1~4, Radix Codonopsis 1~4, Radix Astragali 1~3, Radix Ophiopogonis 1~2 and cactus 1~2.
7. external skin-care composition as claimed in claim 6, it is characterised in that the Chinese medical extract the preparation method comprises the following steps:
(1) it weighs, mixes by the weight after crushing the bulk pharmaceutical chemicals；
(2) percent by volume is the extraction of 60%~90% ethyl alcohol, and bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:8~1:20,
70 DEG C~90 DEG C are extracted 1~4 hour；
(3) extracting solution that step (2) obtains is cooled to 20 DEG C~30 DEG C, 200~400 mesh coarse filtrations carry out after filtering out the dregs of a decoction
Vacuum filtration collects filtrate；
(4) filtrate rotary evaporation whole ethyl alcohol is obtained into concentrate, 1,3-BDO is added in concentrate, additional amount is step
(3) the 60%~90% of gained amount of filtrate percent by volume；It is miscible uniformly, filter to take clear liquid to get.
8. external skin-care composition as claimed in claim 7, which is characterized in that the volume basis of ethyl alcohol in the step (2)
Than being 70%, bulk pharmaceutical chemicals and ethyl alcohol mass volume ratio g/ml are 1:15；Extraction time is 3 hours.
9. external skin-care composition as claimed in claim 7, which is characterized in that vacuum filtration condition is in the step (3)
Two layers of filter paper is spread in Buchner funnel, folder 0.3~0.6cm diatomite is filtered by vacuum among filter paper.
10. the preparation method of external skin-care composition described in a kind of any one of claim 6 to 9, it is characterised in that described
The step of preparation method, is as follows:
(1) first water-soluble jojoba oil is dissolved by heating with water；
(2) EDTA, oat polypeptide, Sodium Hyaluronate, glycerol, allantoin, aloe powder, stimulation inhibiting factor are added to the water, are added
Heat is to stirring after 80 DEG C~90 DEG C to being completely dissolved；
(3) ammonium acryloyl taurate/VP copolymer is added, stirring makes it completely dissolved；
(4) 40 DEG C are cooled to hereinafter, preservative and the Chinese medical extract is added, stir evenly to get.
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| CN103202797A (en) * | 2013-04-15 | 2013-07-17 | 北京华夏众芳生物科技有限公司 | Skin care composition and water aqua having acne treatment efficacy and preparation method of water aqua |
| CN103202892A (en) * | 2013-04-15 | 2013-07-17 | 北京华夏众芳生物科技有限公司 | Traditional Chinese medicine composition and skin care essence having acne treatment efficacy and preparation method of essence |
| CN103417414A (en) * | 2012-05-20 | 2013-12-04 | 李一凡 | Extracting method for cactus primary juice |
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| CN102068606A (en) * | 2010-11-26 | 2011-05-25 | 新时代健康产业（集团）有限公司 | Wrinkle-removing and anti-wrinkle Chinese medicinal composition, preparation and preparation method thereof |
| CN103417414A (en) * | 2012-05-20 | 2013-12-04 | 李一凡 | Extracting method for cactus primary juice |
| CN103202797A (en) * | 2013-04-15 | 2013-07-17 | 北京华夏众芳生物科技有限公司 | Skin care composition and water aqua having acne treatment efficacy and preparation method of water aqua |
| CN103202892A (en) * | 2013-04-15 | 2013-07-17 | 北京华夏众芳生物科技有限公司 | Traditional Chinese medicine composition and skin care essence having acne treatment efficacy and preparation method of essence |
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