CN105130006A - Blue alga removal method - Google Patents
Blue alga removal method Download PDFInfo
- Publication number
- CN105130006A CN105130006A CN201510400443.4A CN201510400443A CN105130006A CN 105130006 A CN105130006 A CN 105130006A CN 201510400443 A CN201510400443 A CN 201510400443A CN 105130006 A CN105130006 A CN 105130006A
- Authority
- CN
- China
- Prior art keywords
- blue
- green algae
- mentioned
- mould
- closed cabinet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a blue alga removal method, and belongs to the field of water environment protection. The alga removal method is provided against the abuse of temporary problem solving of present blue alga processing methods. The method is characterized in that a mixed solution containing cyanotoxins extracted from blue algae and a cyanotoxin and algophagous Aristichthys nobilis internal organ and tissue extract liquid goes moldy, the moldy mixed solution is cultured, and a mold solution is extracted to control blue algae in rivers in order to remove blue algae. Compared with traditional methods, the method used in the invention has the advantages of high blue alga treatment efficiency, low energy consumption, no use of chemical agents, no damages to aquatic animals and plants, and no entrance to human bodies through the food chain.
Description
Technical field
The present invention relates to a kind of blue-green algae minimizing technology, belong to water environment protection field.
Background technology
Along with the develop rapidly of economy, the mankind are also day by day serious to the pollution of water resources.In recent years due to the nutrient laden of water in some inland lakes, annual summer high temperature weather blue-green algae just there will be great outburst, also referred to as wawter bloom; During wawter bloom, on lake surface adrift one deck green, as painted the blue-green algae of shape, be that cubes distributes from the water surface to water body.Anabaena in blue-green algae can produce lethal gene fast, destroys the gill tissue of cultivation object, disturbs it metabolicly normally to carry out, and paralysis is neural, makes it dead.Plant individually not only live body band poison in blue-green algae, and dead individuals decomposition can produce biotoxin---cyanophycean toxin.Cultivation object can be directly caused to be poisoned to death when cyanophycean toxin amount is many, even if quantity is few, also by food chain build-up effect harm cultivation object, until harmful to human.
At present, the main method processing blue-green algae is divided into external environment to administer and environment improvement.Environment is administered needs a large amount of manpower and materials of input to carry out drawing the mode of transferring the pollution such as dirt removal clearly, resettlement contaminating enterprises, does not have fundamentally contaminated solution; Environment improvement aspect, as method and the treatment agent of biological process, all kinds of improvement blue-green algae of chemical method, but is difficult to obtain extensively, effectively applying so far, especially chemical process Chinese medicine adds, the injury of aquatic animals and plants can be caused, also can enter human body by food chain, potential secondary pollution.
Summary of the invention
Technical problem to be solved by this invention: be directed to existing blue-green algae treatment technology and can only take stopgap measures the drawback that can not effect a permanent cure, provide a kind of blue-green algae minimizing technology, mouldy with the extracting solution mixture of food algae bighead internal organ and tissue by extracting cyanophycean toxin from blue-green algae, and the mixing solutions after mouldy is carried out being trained the bacterial strain with molten algae performance, put into region water body, to cultivate blue-green algae for carrier in closed cabinet, water is medium, propagate on neighbouring blue-green algae, so carry out, involve whole region water body, reach the object eradicating blue-green algae.
For solving the problems of the technologies described above, the present invention adopts technical scheme as described below: a kind of blue-green algae minimizing technology, it is characterized in that concrete steps are:
(1) get the water sample 50 ~ 100mL containing blue-green algae, add ethanol wherein, the ethanol added and volume of water sample, than being 3:5 ~ 4:5, are shake 15 ~ 25min under the ultrasonic wave of 300 ~ 450 hertz in frequency;
(2) regulate pH to be 3.0 ~ 4.0 above-mentioned water sample after ultrasonication, filter by nonwoven gauze;
(3) filtrate after nonwoven gauze being filtered carries out centrifugation, gets supernatant liquor;
(4) by above-mentioned supernatant liquor percarbonic acid calcium chromatographic column, be the methanol aqueous solution drip washing of 40% with volume content, then be the methanol aqueous solution wash-out of 95% with volume content, the elutriant of cyanophycean toxin must be contained;
(5) by concentrated for the above-mentioned elutriant containing cyanophycean toxin, distillation, cyanophycean toxin extracting solution is obtained;
(6) get the internal organ of fresh and alive 3 ~ 5 fresh and alive bigheads, repeat above-mentioned (1) ~ (5) step after cleaning up and obtain bighead internal organ and tissue extract;
(7) cyanophycean toxin extracting solution is mixed with bighead internal organ and tissue extract, under the shady and cool condition of humidity, make it mouldy, treat that mould bud grows to 3 ~ 5cm and is advisable;
(8) be inoculated on solid medium by mouldy mixed solution, cultivate 7 ~ 10 days at 25 DEG C of temperature, obtain mould spores culture, described solid medium is agar solid slope;
(9) with sterilized water, mould spores culture is made in inoculation of suspension liquid to tank in sterilized substratum, and pass into sterile air, stirring, at 27 DEG C of temperature, cultivate 20 ~ 28h, obtain seed culture fluid;
(10) then the seed culture fluid obtained is inoculated in the substratum containing toluylic acid precursor, passes into sterile air, stir, and keep temperature to cultivate 5 ~ 8 days, obtain mycin fermented liquid;
(11) by the cooling of above-mentioned mycin fermented liquid, filter, filtrate, under pH is the condition of 2 ~ 2.5, carries out multi-stage counter current extraction with N-BUTYL ACETATE, then regulates with the damping fluid of pH7.0 ~ 7.2, activated carbon decolorizing, through component distillation mould extracting solution;
(12) mould extracting solution heated and boiled obtained above, become gas to pass into and be full of in the closed cabinet of nitrogen, make its in the gas phase concentration be 50 ~ 80ppm, again the blue-green algae just salvaged is put into closed cabinet, naturally 3 ~ 5 days are absorbed, treat the thin out blackout of its color, take out from closed cabinet;
(13) with every 30 ~ 50m
2region area drop into 30 ~ 50g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body.
Application method of the present invention: with every 30 ~ 50m
2region area drop into 30 ~ 50g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body.
Principle of the present invention: also mouldy under certain conditions by the extracting solution mixture extracting cyanophycean toxin and bighead internal organ and tissue from blue-green algae, screening and culturing has the bacterial strain of molten algae performance, put into region water body, to cultivate blue-green algae for carrier in closed cabinet, water is medium, propagates on neighbouring blue-green algae, so carries out, involve whole region water body, reach the object eradicating blue-green algae.
The present invention is compared with additive method, and Advantageous Effects is:
(1) without the use of chemical agent, the injury of aquatic animals and plants can not be caused, also can not enter human body by food chain, potential secondary pollution simultaneously.
(2) compared with traditional method, method process blue-green algae used in the present invention has the characteristic that efficiency is high, energy consumption is low.
Embodiment
A kind of blue-green algae minimizing technology, is characterized in that concrete steps are:
(1) get the water sample 50 ~ 100mL containing blue-green algae, add ethanol wherein, the ethanol added and volume of water sample, than being 3:5 ~ 4:5, are shake 15 ~ 25min under the ultrasonic wave of 300 ~ 450 hertz in frequency;
(2) regulate pH to be 3.0 ~ 4.0 above-mentioned water sample after ultrasonication, filter by nonwoven gauze;
(3) filtrate after nonwoven gauze being filtered carries out centrifugation, gets supernatant liquor;
(4) by above-mentioned supernatant liquor percarbonic acid calcium chromatographic column, be the methanol aqueous solution drip washing of 40% with volume content, then be the methanol aqueous solution wash-out of 95% with volume content, the elutriant of cyanophycean toxin must be contained;
(5) by concentrated for the above-mentioned elutriant containing cyanophycean toxin, distillation, cyanophycean toxin extracting solution is obtained;
(6) get the internal organ of fresh and alive 3 ~ 5 fresh and alive bigheads, repeat above-mentioned (1) ~ (5) step after cleaning up and obtain bighead internal organ and tissue extract;
(7) cyanophycean toxin extracting solution is mixed with bighead internal organ and tissue extract, under the shady and cool condition of humidity, make it mouldy, treat that mould bud grows to 3 ~ 5cm and is advisable;
(8) be inoculated on solid medium by mouldy mixed solution, cultivate 7 ~ 10 days at 25 DEG C of temperature, obtain mould spores culture, described solid medium is agar solid slope;
(9) with sterilized water, mould spores culture is made in inoculation of suspension liquid to tank in sterilized substratum, and pass into sterile air, stirring, at 27 DEG C of temperature, cultivate 20 ~ 28h, obtain seed culture fluid;
(10) then the seed culture fluid obtained is inoculated in the substratum containing toluylic acid precursor, passes into sterile air, stir, and keep temperature to cultivate 5 ~ 8 days, obtain mycin fermented liquid;
(11) by the cooling of above-mentioned mycin fermented liquid, filter, filtrate, under pH is the condition of 2 ~ 2.5, carries out multi-stage counter current extraction with N-BUTYL ACETATE, then regulates with the damping fluid of pH7.0 ~ 7.2, activated carbon decolorizing, through component distillation mould extracting solution;
(12) mould extracting solution heated and boiled obtained above, become gas to pass into and be full of in the closed cabinet of nitrogen, make its in the gas phase concentration be 50 ~ 80ppm, again the blue-green algae just salvaged is put into closed cabinet, naturally 3 ~ 5 days are absorbed, treat the thin out blackout of its color, take out from closed cabinet;
(13) with the region area of every 30 ~ 50m2 drop into 30 ~ 50g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body.
Example 1
With every 30m
2region area drop into 30g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body; In input after 3 days, in river course there is large stretch of death in blue-green algae, and its mortality ratio reaches more than 80%.
Example 2
With every 40m
2region area drop into 40g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body; In input after 4 days, in river course there is large stretch of death in blue-green algae, and its mortality ratio reaches more than 82%.
Example 3
With every 50m
2region area drop into 50g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body; In input after 5 days, in river course there is large stretch of death in blue-green algae, and its mortality ratio reaches more than 85%.
Claims (1)
1. a blue-green algae minimizing technology, is characterized in that concrete steps are:
(1) get the water sample 50 ~ 100mL containing blue-green algae, add ethanol wherein, the ethanol added and volume of water sample, than being 3:5 ~ 4:5, are shake 15 ~ 25min under the ultrasonic wave of 300 ~ 450 hertz in frequency;
(2) regulate pH to be 3.0 ~ 4.0 above-mentioned water sample after ultrasonication, filter by nonwoven gauze;
(3) filtrate after nonwoven gauze being filtered carries out centrifugation, gets supernatant liquor;
(4) by above-mentioned supernatant liquor percarbonic acid calcium chromatographic column, be the methanol aqueous solution drip washing of 40% with volume content, then be the methanol aqueous solution wash-out of 95% with volume content, the elutriant of cyanophycean toxin must be contained;
(5) by concentrated for the above-mentioned elutriant containing cyanophycean toxin, distillation, cyanophycean toxin extracting solution is obtained;
(6) get the internal organ of fresh and alive 3 ~ 5 fresh and alive bigheads, repeat above-mentioned (1) ~ (5) step after cleaning up and obtain bighead internal organ and tissue extract;
(7) cyanophycean toxin extracting solution is mixed with bighead internal organ and tissue extract, under the shady and cool condition of humidity, make it mouldy, treat that mould bud grows to 3 ~ 5cm and is advisable;
(8) be inoculated on solid medium by mouldy mixed solution, cultivate 7 ~ 10 days at 25 DEG C of temperature, obtain mould spores culture, described solid medium is agar solid slope;
(9) with sterilized water, mould spores culture is made in inoculation of suspension liquid to tank in sterilized substratum, and pass into sterile air, stirring, at 27 DEG C of temperature, cultivate 20 ~ 28h, obtain seed culture fluid;
(10) then the seed culture fluid obtained is inoculated in the substratum containing toluylic acid precursor, passes into sterile air, stir, and keep temperature to cultivate 5 ~ 8 days, obtain mycin fermented liquid;
(11) by the cooling of above-mentioned mycin fermented liquid, filter, filtrate, under pH is the condition of 2 ~ 2.5, carries out multi-stage counter current extraction with N-BUTYL ACETATE, then regulates with the damping fluid of pH7.0 ~ 7.2, activated carbon decolorizing, through component distillation mould extracting solution;
(12) mould extracting solution heated and boiled obtained above, become gas and pass into and be full of in the closed cabinet of nitrogen, make its in the gas phase concentration be 50 ~ 80ppm, again the blue-green algae just salvaged is put into closed cabinet, naturally absorb 3 ~ 5 days, treat the thin out blackout of its color, take out from closed cabinet;
(13) with every 30 ~ 50m
2region area drop into 30 ~ 50g above-mentioned from closed cabinet after mould process thin out blackout blue-green algae calculate, subregion covers whole region water body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510400443.4A CN105130006A (en) | 2015-07-09 | 2015-07-09 | Blue alga removal method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510400443.4A CN105130006A (en) | 2015-07-09 | 2015-07-09 | Blue alga removal method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105130006A true CN105130006A (en) | 2015-12-09 |
Family
ID=54715665
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510400443.4A Pending CN105130006A (en) | 2015-07-09 | 2015-07-09 | Blue alga removal method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105130006A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943437A (en) * | 2017-03-06 | 2017-07-14 | 广东药科大学 | A kind of Microcystis aeruginosa ethanol extract and preparation method and application |
-
2015
- 2015-07-09 CN CN201510400443.4A patent/CN105130006A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943437A (en) * | 2017-03-06 | 2017-07-14 | 广东药科大学 | A kind of Microcystis aeruginosa ethanol extract and preparation method and application |
| CN106943437B (en) * | 2017-03-06 | 2020-01-03 | 广东药科大学 | Ethanol extract of microcystis and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103992187B (en) | A kind of bio-bacterial manure for preventing and treating water body moss and preparation method thereof | |
| CN102876615B (en) | Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide | |
| CN104585097A (en) | Circulating aquaculture device and method | |
| CN103484370B (en) | A kind of method utilizing farm animal excrement waste water to cultivate spirulina | |
| CN103937726A (en) | Alga-lysing pseudomonas aeruginosa and application thereof | |
| CN103695353A (en) | Bacillus subtilis with capability of degrading ammonia nitrogen and application of bacillus subtilis | |
| WO2023000612A1 (en) | Fungicide for botryodiplodia theobromae and use thereof | |
| CN102899277B (en) | Bacillus cereus and application thereof | |
| CN108651522A (en) | A kind of vibrio alginolyticus phage preparation, preparation method and applications | |
| CN112501090B (en) | Bacillus licheniformis and application thereof | |
| CN103074279B (en) | Paenibacillus and application thereof in degrading microcystin | |
| CN103773690B (en) | A kind of method of open cultivation microalgae | |
| CN110484472B (en) | Klebsiella and application thereof | |
| CN103352010B (en) | Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria, and its application | |
| CN103103127B (en) | Culture method for microalgae | |
| CN105130006A (en) | Blue alga removal method | |
| CN106865804B (en) | Clean production method of Zhongshengmycin mother medicine | |
| CN105296355A (en) | Environmental microorganism bacterium extraction and propagation compositions and using methods thereof | |
| CN105110489A (en) | Water-purifying and weed-protecting biological agent for shrimp and crab culture in high-temperature period as well as preparation method and application of biological agent | |
| CN106259511B (en) | A kind of biological algicide | |
| CN101967463B (en) | Method for improving spore germination rate of beauveria bassiana by adding nutrients | |
| CN103952359A (en) | Brevundimonas sp. and application thereof | |
| CN106614810A (en) | Potamogeton malaianus-sodium alginate pellet-type algistat as well as preparation method and application thereof | |
| CN106190898A (en) | A kind of industrialization liquid fermentation method of the bacillus cereus JZBC1 dissolving pond dinoflagellate | |
| CN105400697A (en) | Method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in carbon dioxide environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151209 |