CN104988059B - A kind of bioreactor for algal culture - Google Patents
A kind of bioreactor for algal culture Download PDFInfo
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- CN104988059B CN104988059B CN201510256337.3A CN201510256337A CN104988059B CN 104988059 B CN104988059 B CN 104988059B CN 201510256337 A CN201510256337 A CN 201510256337A CN 104988059 B CN104988059 B CN 104988059B
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
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- C12M31/00—Means for providing, directing, scattering or concentrating light
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Abstract
The present invention relates to field of biotechnology more particularly to a kind of bioreactor for algal culture, including reactor body, separating element and aeration equipment, reactor body is back cover tubulose, is made of clear material；Separating element is located at the inside of the reactor body, reactor body is divided into two spaces, there are the channels for the connection of left and right two spaces for the top and bottom of separating element；Aeration equipment is connected to the bottom in any space of the reactor body, up-draught.Bioreactor is resolved by the four-dimensional circulating mixing of algae solution for temperature control, the four major problem that algae solution is mixed, carbon source provides, oxygen discharges at any time in microalga cultivation process through the invention.The gas being passed through causes the difference of front and back two parts algae solution specific gravity, increases mixing efficiency in this way, increases the function of ventilation, reduces the consumption of tolerance, increases path and the time of its reactive absorption carbon, creates suitable environment for microalgae.
Description
Technical field
The present invention relates to field of biotechnology more particularly to a kind of bioreactors for algal culture.
Background technique
" the efficient large-scale cultivation technology of microalgae " is one of core of Microalgae biotechnology at present.The reaction of closed photo object
Device, i.e., closed culture systems are the bioreactors built with transparent material.This bioreactor is in addition to that can acquire luminous energy
Outside, there are many similarities for Miscellaneous and traditional microbial fermentation bioreactor.The reaction of closed photo object
Microalgae single, purebred culture may be implemented in device, and condition of culture is easily controllable, and culture density is high, easily harvests, so efficiency
It is higher, but build and also increased accordingly with operating cost.
Such as Chinese patent ZL02134235.0 discloses a kind of automatic continuous production tubular type bioreactor, it
Reaction vessel be transparent translucent material cylindrical type communicating pipe, have arrival end and outlet end, and access blending tank, reactor be by
Multilayer U-typed communicating pipe it is cross-superimposed and formed three-dimensional pipeline, light source be located at communicating pipe it is cross-superimposed and formation " # " font
In solid space.This both culturing microalgae mode and equipment are although overcome the extensive disadvantage in open pond, and structure is complicated, on a large scale
Device is difficult to realize, and construction cost is high, and is difficult to make full use of natural sunlight, is unsuitable for microalgae low cost, large-scale is supported
It grows.
Chinese patent ZL03128138.9 discloses a kind of closing tubular type bioreactor, by three-dimensional double flat spin formula
Pipeline and unique U-shaped connecting bend, double tower, zero shearing force infusion pump, carbon dioxide gas injection device, the structures such as cool-heat-exchanger
At.Deoxygenation reaction tower in double tower is equipped with negative pressure jet pump, can effectively exclude the accumulation oxygen in culture solution, and regulation tower can reconcile
It is hydraulic and culture solution is conveyed to reacting pipe with negative pressure automatically.The reactor overcomes conventional reactor and takes up a large area, efficiency
Large-scale production may be implemented in low disadvantage, but structure is complicated, and manufacturing cost is high.And reactor is erect and is placed, culture solution
Need very big energy consumption to be transported to top from bottom with algae solution, the shearing force of algal filament greatly increased, also increase both culturing microalgae at
This.
Chinese patent CN1721523A discloses a kind of bioreactor of microalgae mass culture, including transparent pipeline,
The compositions such as gas resolver, auxiliary pipeline system, culture parameters impression and control facility.Using large-scale gas resolver,
Transparent pipeline arranged in parallel is subjected to parallel connection, solves the problems, such as closed conduct bioreactor gas exchanges, but it is same
Sample has that manufacturing cost and operating cost are high.
Chinese patent CN1668185A is disclosed at the method handled using bioreactor to gas and gas
Reason system can make microalgae reach maximum growth rate, effective absorbing carbon dioxide/or nitrogen oxygen according to the methods and apparatus disclosed
Compound.However, the system structure is complicated, computerized control although adopting, large-scale production necessarily cause device fabrication at
This is high, and energy consumption is high, is unsuitable for the acquisition that microalgae biomass is a large amount of, inexpensive.
Chinese patent ZL96216364.3 discloses a kind of latent layer spirulina cultivation apparatus of hermetic type circulation, is sprayed by overflow
Device, the board-like bioreactor of overflow, reservoir, circulating pump are connected in sequence, wherein using the overflow of translucent material production
Board-like bioreactor is provided with the supporting plate with baffle of multiple-layer horizontal placement and the overflow port cross-distribution of upper and lower level.This is anti-
Although answering device efficiency very high, multilayer support board structure is sufficiently complex, is unfavorable for amplifying production on a large scale.
Therefore, against the above deficiency, it is desirable to provide a kind of efficiency of light energy utilization height, energy-saving low-carbon, make full use of the natural resources,
The algal culture bioreactor of microalgae high production efficiency.
Summary of the invention
(1) technical problems to be solved
The technical problem to be solved by the present invention is to solve algal culture to produce due to that cannot discharge photosynthesis in time in the process
Raw oxygen is suppressed the problem of low with microalgae production efficiency so as to cause photosynthesis and natural lighting area is small, light
Energy utilization rate is low, existing equipment is complicated, energy consumption is high and problem at high cost.
(2) technical solution
In order to solve the above-mentioned technical problems, the present invention provides a kind of bioreactors for algal culture, including
Reactor body, separating element and aeration equipment, the reactor body are back cover tubulose, are made of clear material；Described point
It is located at the inside of the reactor body every unit, the reactor body is divided into left and right two spaces, the separating element
Top and bottom there are for left and right two spaces connection channel；Times of the aeration equipment and the reactor body
The bottom in one space is connected to, up-draught.
Wherein, the cross section of the reactor body is that equal portions are quincunx.
Wherein, the separation device is lamina of septum pellucidum.
Wherein, the separating element is the transparent calandria structure that row's vertical tube is in turn connected to form, and each vertical tube upper and lower end is close
Envelope, is arranged light source in the vertical tube in middle position.
Wherein, the vertical tube by marginal position of the transparent calandria structure is long tube, and long tube and reactor body inner wall paste
Tightly, the vertical tube by middle position of the transparent calandria structure is short tube, is formed with the top and bottom in transparent calandria structure
For the channel of left and right two spaces connection.
Wherein, the length of the short tube is shorter by 10% to 40% than the length of the long tube；The light source is LED light.
Wherein, the bottom of the reactor body is equipped with fluid guidance port, and top is equipped with the top cover with inlet.
Wherein, the aeration equipment includes sequentially connected gas source, gas-guide tube and air nozzle, and the gas-guide tube is by described anti-
It answers generator top to open below into the reaction generator bottom along the long tube to connect with the air nozzle.
Wherein, the gas source is the wind power air compressor being connected and compressed air storage tank, the compressed air storage
Hiding tank is connect with the gas-guide tube.
Wherein, the air nozzle is micropore gas disperser；The reactor body is equipped with metab.
(3) beneficial effect
Above-mentioned technical proposal of the invention has the advantages that reactor body is divided into two by separating element by the present invention
Space, a space bottom are connected with aeration equipment up-draught, since the gas being passed through causes the algae solution specific gravity in this space to subtract
It is small, so that the algae solution of two spaces is formed the difference of specific gravity, the algae solution of ventilation space runs to top by staying at the top of two spaces
Some channels as downward liquid stream is run down into non-ventilation space, and then run to bottom and pass through two spaces bottom
There are channel, as liquid stream enters ventilation space, the algae solution four-dimension circulation mixing of the front and back up and down formed in this way it is different with pass
The gas-lifting type of system mixes, and increases mixing efficiency, increases ventilatory function, reduces the consumption of tolerance.If be passed through containing two
The gas of carbonoxide also increases path and the time of its reactive absorption carbon, creates suitable environment for microalgae, it is often more important that
It solves the problems, such as to discharge oxygen at any time in microalga cultivation process using the gas being passed through, so being passed through bioreactor
Compressed air at least algae solution mixing, carbon source offer, algae solution temperature control and the effect that oxygen and gas exchanges are discharged at any time；
The design of bioreactor shape plum blossom shape tube considerably increases the efficiency of light energy utilization compared with the design of traditional round tube；And it is logical
Wind power air compressor and the interior transparent calandria structure for setting light source are crossed, wind energy and luminous energy are incorporated into photo-bioreactor system, it is real
The high microalgae high-density breeding of controllable both culturing microalgae condition, low-carbon green, the efficiency of light energy utilization is showed.
In addition to it is described above present invention solves the technical problem that, constitute technical solution technical characteristic and have this
Except advantage brought by the technical characteristic of a little technical solutions, the other technical characteristics of bioreactor of the present invention and these skills
The advantage that art characteristic strip comes, will further illustrate in conjunction with attached drawing.
Detailed description of the invention
Fig. 1 is overall construction drawing of the embodiment of the present invention one for the bioreactor of algal culture；
Fig. 2 is the embodiment of the present invention one for reactor body perspective view in the bioreactor of algal culture；
Fig. 3 is reactor cross-sectional view of the embodiment of the present invention one for the bioreactor of algal culture；
Fig. 4 is overall construction drawing of the embodiment of the present invention two for the bioreactor of algal culture；
Fig. 5 is reactor cross-sectional view of the embodiment of the present invention two for the bioreactor of algal culture.
In figure: 1: reactor body；2: Bian Guan；3: short tube；4: gas-guide tube；5: air nozzle；6: inlet；7: top cover；8:
Separating element；9: aeration equipment；10: fluid guidance port；11: gas source.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill people
Member's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
Embodiment one
As shown in Figure 1, the bioreactor for algal culture that the embodiment of the present invention one provides, including reactor master
Body 1, separating element 8 and aeration equipment 9, as shown in Figures 2 and 3, reactor body 1 are back cover tubulose, and cross section is equal part plum
Flower-shape is made of clear material, and such structure and traditional round tube design considerably increase illuminating area and the efficiency of light energy utilization, is made
It increases 50-70%；1 main body of reactor is divided into two positioned at the inside of reactor body 1 for lamina of septum pellucidum by separating element 8
Space, there are the channel for the connection of left and right two spaces, partition can not influence to be transparent for the top and bottom of lamina of septum pellucidum
Illumination.
Specifically, aeration equipment 9 includes sequentially connected gas source 11, gas-guide tube 4 and air nozzle 5；Further gas source 11
For the air compressor and compressed air storage tank being connected, compressed air storage tank is connect with gas-guide tube 4, and gas-guide tube 4 is by anti-
It answers and opens below into 1 bottom of reactor body at the top of device main body 1 and connect with air nozzle 5, micropore gas dispersion may be selected in air nozzle 9
Device, uniformly continuous up-draught；Setting compressed air storage tank can store gas.
Generally, the bottom of reactor body 1 is equipped with fluid guidance port 10, and the algae solution cultivated can be flowed out by the fluid guidance port,
Into the next step；Top is equipped with the top cover 7 with inlet 6, can prevent sundries from falling into reactor body 1, with algae
Liquid pollutes；Reactor body 1 can set metab when using, and reactor is placed on metab.
Since the gas being passed through causes the difference of algae solution specific gravity in two parts space of front and back, runs to top and enter the other half
In space and as downward liquid stream is run down, mixing efficiency has been greatly increased in this way, reduces the consumption of tolerance.If logical
Enter the gas containing carbon dioxide and also increase path and the time of its reactive absorption carbon, it is often more important that utilizes the gas being passed through
It solves the problems, such as temperature control during algal culture and discharges oxygen at any time.
Embodiment two
As shown in Figure 4 and Figure 5, a kind of bioreactor and reality for algal culture provided by Embodiment 2 of the present invention
It applies one main distinction of example and is that separating element 8 is the transparent calandria structure that row's vertical tube is in turn connected to form, be located at reactor master
1 main body of reactor is divided into two spaces by the inside of body 1, and there are supply left and right two for the top and bottom of transparent calandria structure
Light source is arranged in the channel of space connection, each vertical tube upper and lower end sealing in the vertical tube in middle position, and light source can be LED light,
Make to obtain uniform illumination inside reactor body 1, is conducive to the better photosynthesis of algae, and the light source is controllable, makes microalgae
Under good illumination condition.
Specifically, the vertical tube by marginal position of transparent calandria structure is long tube 2, long tube 2 and 1 inner wall of reactor body are pasted
Tightly, light source can be set inside, light source can also be not provided with, because leaning on proximal edge, there is corresponding natural lighting environment, lead
Tracheae is connect by opening below into reaction generator bottom along the long tube at the top of reaction generator with air nozzle；Transparent calandria structure
The vertical tube by middle position be short tube 3, with the top and bottom of transparent calandria structure formed for left and right two spaces be connected to
Channel, the length of short tube 3 is shorter by 10% to 40% than the length of long tube 2.
In conclusion reactor body is divided into two spaces by separating element by the present invention, a space bottom is connected with logical
Gas equipment up-draught, this half space form the algae solution containing a large amount of microbubbles and run up, this half space of ventilating
Algae solution run to top by the top of two spaces there are channel, as downward liquid stream runs down one that entrance is not ventilated
Half space, so run to bottom by two spaces bottom there are channel, as liquid stream enters the space of ventilation, like this
Liquid constantly supplements downwards, formed algae solution circulation state, reach algal filament uniformly mix, be discharged oxygen, algae solution regulating and controlling temperature and
The effect of the gradient of nutrition, illumination and temperature in elimination reaction device.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (7)
1. a kind of bioreactor for algal culture, including reactor body, separating element and aeration equipment, feature
Be: the reactor body is back cover tubulose, is made of clear material；The separating element is located at the reactor body
The reactor body is divided into left and right two spaces by inside, and there are for left and right for the top and bottom of the separating element
The channel of two spaces connection；The aeration equipment is connected to the bottom in any space of the reactor body, up-draught；
The cross section of the reactor body is that equal portions are quincunx；The separating element is the transparent row that row's vertical tube is in turn connected to form
Pipe structure, each vertical tube upper and lower end sealing, is arranged light source in the vertical tube in middle position.
2. bioreactor according to claim 1, it is characterised in that: the transparent calandria structure leans on marginal position
Vertical tube be long tube, long tube is adjacent to reactor body inner wall, and the vertical tube by middle position of the transparent calandria structure is short
Pipe, to form the channel for the connection of left and right two spaces in the top and bottom of transparent calandria structure.
3. bioreactor according to claim 2, it is characterised in that: the length of the short tube is longer than the long tube
Spend short 10% to 40%；The light source is LED light.
4. bioreactor according to claim 1, it is characterised in that: the bottom of the reactor body is equipped with drain
Mouthful, top is equipped with the top cover with inlet.
5. bioreactor according to claim 2, it is characterised in that: the aeration equipment includes sequentially connected gas
Source, gas-guide tube and air nozzle, the gas-guide tube is by opening below into the reaction along the long tube at the top of the reaction generator
Generator bottom is connect with the air nozzle.
6. bioreactor according to claim 5, it is characterised in that: the gas source is the wind-force air pressure being connected
Contracting machine and compressed air storage tank, the compressed air storage tank are connect with the gas-guide tube.
7. bioreactor according to claim 5, it is characterised in that: the air nozzle is micropore gas disperser；
The reactor body is equipped with metab.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510256337.3A CN104988059B (en) | 2015-05-19 | 2015-05-19 | A kind of bioreactor for algal culture |
| PCT/CN2016/082541 WO2016184394A2 (en) | 2015-05-19 | 2016-05-18 | Photobioreactor used for algae cultivation, and algae cultivation system |
| US15/575,282 US11319522B2 (en) | 2015-05-19 | 2016-05-18 | Photobioreactor used for algae cultivation, and algae cultivation system |
| JP2018512471A JP6570736B2 (en) | 2015-05-19 | 2016-05-18 | Algal aquaculture photobioreactor and algae culture system |
| KR1020177036632A KR102043623B1 (en) | 2015-05-19 | 2016-05-18 | Photobioreactors for algal farming and algal farming systems |
| ZA2017/08668A ZA201708668B (en) | 2015-05-19 | 2017-12-19 | Photobioreactor used for algae cultivation, and algae cultivation system |
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| CN201510256337.3A CN104988059B (en) | 2015-05-19 | 2015-05-19 | A kind of bioreactor for algal culture |
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| CN104988059B true CN104988059B (en) | 2019-01-04 |
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| JP6570736B2 (en) * | 2015-05-19 | 2019-09-04 | 何 忠志 | Algal aquaculture photobioreactor and algae culture system |
| CN106434278B (en) * | 2016-08-26 | 2019-10-18 | 浙江天地环保科技有限公司 | A kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors |
| FR3075815B1 (en) * | 2017-12-22 | 2020-08-07 | Suez Groupe | PHOTOBIOREACTOR |
| CN109554277B (en) * | 2018-12-26 | 2024-09-24 | 博生微藻(北京)科技有限公司 | Photobioreactor microalgae cultivation system and pipe network system |
| CA3126900A1 (en) * | 2019-01-15 | 2020-07-23 | Yemoja Ltd. | Scalable production and cultivation systems for photosynthetic microorganisms |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2009069967A2 (en) * | 2007-11-28 | 2009-06-04 | Inha-Industry Partnership Institute | Photobioreactor for large-scale culture of microalgae |
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| BE1017763A5 (en) * | 2007-09-24 | 2009-06-02 | Proviron Holding | BORE ACTOR. |
| CN201485462U (en) * | 2009-07-24 | 2010-05-26 | 浙江省海洋水产养殖研究所 | Accelerator for culturing microalgae |
| CN103255046B (en) * | 2012-02-16 | 2014-06-04 | 中国石油天然气股份有限公司 | Circulating foam photobioreactor and application thereof |
| CN204737957U (en) * | 2015-05-19 | 2015-11-04 | 北京林业大学 | A photobioreactor for algae culture |
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| WO2009069967A2 (en) * | 2007-11-28 | 2009-06-04 | Inha-Industry Partnership Institute | Photobioreactor for large-scale culture of microalgae |
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