CN104861067A

CN104861067A - Compound for immunotherapy, and composition

Info

Publication number: CN104861067A
Application number: CN201410011324.5A
Authority: CN
Inventors: 李立新
Original assignee: Bo Di Bio Tech Ltd
Current assignee: Bo Di Bio Tech Ltd
Priority date: 2014-01-10
Filing date: 2014-01-10
Publication date: 2015-08-26

Abstract

The present invention relates to a compound for targeting immunotherapy, and a composition containing the compound. The present invention further relates to applications of the compound in treatment of diseases such as cancers.

Description

Compounds and compositions for immunotherapy

Technical Field

The present invention relates to compounds for targeted immunotherapy and compositions containing the same. The invention also relates to the use of said compounds in the treatment of diseases such as cancer.

Background

Therapeutic antibodies have been used clinically for over twenty years. Fifteen antitumor antibody drugs are currently used clinically, including: rituxan (1997), Herceptin (1998), Mylotarg (2000), Campath (2001), Zevalin (2002), Bexxer (2003), Avastin (2004), Erbitux (2004), Vectibix (2006), Arzerra (2009), Benlysta (2011), Yervoy (2011), Adcetris (2011), Perjeta (2012), and Kadcyla (2013). These antibodies target mainly four molecules: EGFR, Her2, CD20 and VEGF.

In general, therapeutic antibodies kill tumor cells by three mechanisms (Scott AM, Wolchok JD, Old lj. antibody therapy of Cancer. nat Rev Cancer. (2012),12: 278-87): (1) antibodies act directly, i.e., block or agonize ligand/receptor signaling activity, induce apoptosis and deliver drugs or cytotoxic agents. The antibody receptor activation activity can produce a direct killing effect on tumor cells. For example, some antibodies can bind to receptors on the surface of tumor cells, activate the receptors, and cause apoptosis (e.g., in the mitochondria). Antibodies can also mediate tumor cell killing by receptor antagonistic activity. For example, some antibodies can bind to cell surface receptors and block dimerization, kinase activation, and downstream signal transduction, thereby inhibiting proliferation and promoting apoptosis. Binding of antibodies to enzymes can lead to neutralization, signal blocking, and cell death. (2) Immune-mediated cell killing mechanisms including complement-dependent cytotoxicity (CDC), antibody-dependent cell-mediated cytotoxicity (ADCC), modulation of T-cell function, and the like. Immune-mediated killing of tumor cells can be accomplished by: induction of phagocytosis, activation of complement, antibody-dependent cell-mediated cytotoxicity, targeting of genetically modified T cells to tumors by single-chain variable fragments (scFv), activation of T cells by antibody-mediated antigen cross-presentation by dendritic cells, inhibition of T cell inhibitory receptors (e.g., cytotoxic T lymphocyte-associated antigen 4 (CTLA 4)). Among these, the properties of the Fc portion of antibodies are particularly important for CDC and ADCC mediated killing of tumor cells. (3) The specific effects of antibodies on tumor vasculature and stroma by capturing a vascular receptor antagonist or ligand to induce vascular and stromal cell ablation, comprising: inhibition of stromal cells, delivery of toxins to stromal cells, and delivery of toxins to the vasculature (Scott AM, Wolchok JD, Old lj. antibody therapy of cancer. nat Rev cancer.2012,12(4): 278-87).

Therapeutic monoclonal antibody drugs have advanced the research and development of anti-cancer drugs. However, there are still some problems to be solved by further research, such as immunogenicity of the antibody, tolerance to long-term use of tumor targets, and simply single blocking of the long-term effects of the signal transduction pathway. In short, most antibodies have difficulty achieving long-term effective inhibition and killing of tumor cells.

In 1964, the journal "Nature" issued a new view of antibody-drug conjugation (ADC) technology, which was developed in breakthrough in recent years. ADCs covalently link an antibody to a highly toxic drug (toxin) through a chemical linker (linker). The antibody recognizes the cancer cell surface antigen molecule, endocytosis brings the ADC into the cytoplasm, and specifically, intracellular environmental toxins released after linker hydrolysis kill the cell.

Brentuximab Vedotin (trade name Adcetris) a drug has been developed by Seattle Genetics, which has been approved by the FDA for marketing. It is monomethyl auristatin E (MMAE), a synthetic toxic anticancer drug, linked to antibodies targeting lymphoma cell-specific CD30 molecules, with improved tumor cell killing utility.

Currently, clinical trials have been conducted on dozens of such ADC drugs. Among them, Genentech and Immunogen have developed trastuzumab coupled with maytansine (maytansine), a drug known as ado-trastuzumab emtansine (Kadcyla), also known as T-DM1, for the treatment of breast cancer in combination. In 2 months 2013, FDA has approved T-DM1 for human epidermal growth factor receptor2 (Her 2) -positive metastatic breast cancer. Maytansine is a small molecule toxin that binds to tubulin and prevents microtubule formation by forming a non-reducing bis-maleimide-propylene glycol complex. Trastuzumab works on breast and gastric cancers by targeting human Her 2. Trastuzumab has been approved for Her 2-positive cancers. Trastuzumab, however, failed to promote apoptosis in all Her 2-positive cells. T-DM1 binds trastuzumab, which selectively targets the Her2 receptor, to the potent cytotoxic agent maytansine, killing tumor cells. The T-DM1 antibody binds to Her2 receptor, resulting in intracellular internalization of maytansine released from the conjugate, thereby killing the tumor cells. T-DM1 has better overall therapeutic effect, pharmacokinetic properties and lower toxicity.

The traditional small molecule chemotherapeutic drug has strong toxicity and pharmacokinetic advantages, but the traditional small molecule chemotherapeutic drug can affect other physiological targets in the process of treating tumors, and serious side effects are generated. Antibody-drug conjugates allow for the binding of targeting effects to small molecule drugs with specific pharmacokinetics. The structure of the antibody-drug conjugate is the linkage of a monoclonal antibody with a targeting function to a compound with specific pharmacological properties. This technique requires specific binding of the therapeutic antibody to the target, coupled with molecules having therapeutic or other functions such as cytotoxins. Many factors influence the action of this type of antibody, such as endocytosis of the conjugated antibody, stability of the conjugation, and release and killing activity of the toxin.

Toxin molecules currently in use include the tubulin inhibitor Auristatin analogue monomethyl Auristatin E, monomethyl Auristatin F and maytansine. Monomethyl auristatin E is a synthetic microtubule polymer inhibitor that inhibits microtubule aggregation, interferes with tumor cell mitosis, and induces apoptosis (Naumovski L and Junutula JR. Glembatuzumab vesicle, a conjugate of an anti-glucoprotein non-metastatic melanoma protein B mAb and monomethylauristatin E for a molecular of a melanoma and Breast cancer. Current Optin Mol Ther2003;12(2):248-57.Francisco, JARVE et al, cAC10-vCMMAE, an anti-CD 30-monomethylauristatin E conjugate with a pore and selection antibody blood organism 102(4): 1458-14565). Monomethyl Auristatin F is an antimitotic Auristatin derivative having a charged phenylalanine residue at the C-terminus. Compared to uncharged MMAE, monomethyl auristatin F minimizes disruption of cellular signaling pathways and minimizes cytotoxicity. A number of CD30 cell tests found that mAb-maleimidocaproyl-valine-citrulline-p-aminobenzyloxycarbonyl-MMAF (mAb-L1-MMAF) was 2,200 times more toxic than MMAF alone (Doronina SO et al, Enhanced activity of monomethylenical auristatin F through monoclonal antibody delivery: effects of linker technology on efficiency and sensitivity. bioconjugate Chem,2006;17(1): p 114-24). Maytansine is an antimitotic agent that acts as an inhibitor of tubulin polymerization, interfering with the formation of microtubules within the nucleus. Maytansine also inhibits DNA, RNA and protein synthesis, and maytansine has been found to have the greatest effect on DNA synthesis.

The antibody-drug conjugate has direct and indirect anticancer effects. The antibody blocks or activates ligand/receptor signaling, induces apoptosis, and at the same time the antibody can present or deliver a payload drug (e.g., a drug, toxin, small interfering RNA, or radioisotope) to the tumor cell, either directly or indirectly. Therapeutic antibody drug conjugates use the dual properties of an antibody and a conjugated drug, the first being a binding function for specific binding to a target molecule, the second being a tumor cell killing function of the antibody itself, and the third being a specific action of the conjugated drug. The currently used antibody-drug conjugate drugs are limited in how to directly kill tumor cells. However, difficulties remain in practical clinical studies due to stringent technical requirements in terms of antibodies, linker molecules, toxin molecules, conjugation, and the limited number of molecules that can carry toxins into the tumor microenvironment.

Disclosure of Invention

In one aspect, the present invention provides a compound having the structure of formula (Ia):

TM-Ln-AM （Ia），

wherein TM is a targeting moiety, AM is an activating moiety capable of activating human immune cells, Ln is a linker, n is an integer selected from 0 and 1, including but not limited to: dendritic cells, macrophages, monocytes, myeloid suppressor cells, NK cells, B cells, T cells or tumor cells or combinations thereof.

In some embodiments, the human dendritic cell is a plasmacytoid dendritic cell. In some embodiments, the human dendritic cell is a myeloid dendritic cell.

In some embodiments, AM is capable of specifically binding to human toll-like receptor 7 (TLR 7) and/or human TLR8 or is capable of activating human immune cells through TLR7 and/or TLR 8.

In another aspect, the present invention provides a compound having the structure of formula (Ib):

TM-L-AM （Ib），

wherein TM is a targeting moiety, L is a linker, and AM is an activating moiety represented by the structure of the following general formula (I):

wherein the dotted line indicates the presence or absence of a chemical bond,is a point to be connected to a connector;

x is S or-NR₁，R₁is-W₀—W₁—W₂—W₃—W₄；

W₀Is a bond, alkyl, alkenyl, alkynyl,alkoxy or-alkyl-S-alkyl- -,

W₁is a chemical bond, - -O- - -, or- -NR₂- - -, wherein R is₂Is a hydrogen, alkyl or alkenyl group,

W₂is a chemical bond, -O-, -C (O) -, -C (S) -or-S (O)₂—，

W₃Is a chemical bond, - -NR₃- - -, wherein R is₃Is a hydrogen, alkyl or alkenyl group,

W₄is hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, aryl, aryloxy, heteroaryl or heterocyclyl, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, -NH₂Nitro, -alkyl-hydroxy, -alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄-O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，-C(O)-O-R₄，--S-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，-NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN, - -NO₂and-SH, wherein R₄Independently hydrogen, alkyl, alkenyl, - -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl;

z is hydrogen, alkyl, alkenyl, alkynyl, alkoxy, aryl, haloalkyl, heteroaryl, heterocyclyl, each of which may be optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclyl, halo, cyano, nitro, - - -N (R)₅)₂- - (O) -alkyl, - -alkoxy-alkenyl, - -C (O) -alkyl, - -C (O) -O-alkyl, - -O-C (O) -alkyl, - -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl and- -CO-heteroaryl, where R is₅Each independently is hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkyl-heteroaryl;

r is hydrogen, alkyl, alkoxy, haloalkyl, halogen, aryl, heteroaryl, heterocyclyl, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, - - -NH₂Nitro, -alkyl-hydroxy, -alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄，--C(O)-NH-R₄，--C(O)-NR₄R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，--C(O)-O-R₄，--O-C(O)-R₄，--S-R₄，--C(O)-S-R₄，--S-C(O)-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，--NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN and- -SH, in which R₄Independently hydrogen, alkyl, alkenyl, alkoxy, -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl, or haloalkyl;

n is 0,1, 2,3 or 4;

y is-NR₆R₇，–CR₆R₇R₈or-alkyl-NH₂Each of which may be optionally substituted with one or more substituents selected from: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, - -NH₂Halogen, - -N (R)₅)₂-alkoxy-alkyl, -alkoxy-alkenyl, -C (O) -alkyl, -C (O) -O-alkyl, -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl and- -CO-heteroaryl,

wherein R is₆，R₇And R₈Independently hydrogen, alkyl, alkenyl, alkoxy, alkylamino, dialkylamino, alkylthio, arylthio, - -alkyl-hydroxy, - -alkyl-C (O) -O-R₉- - (O) -R- -alkyl-C₉or-alkyl-O-C (O) -R₉Wherein R is₅Each independently is hydrogen, alkyl, haloalkyl, - -alkyl-aryl or-alkyl-heteroaryl, wherein R₉Is hydrogen, alkyl, alkenyl, halogen or haloalkyl;

optionally, X and Z together may form a 5 to 9 membered ring.

In some embodiments, L is a linker represented by the structure of the following general formula (II):

m is 1,2,3, 4,5 or 6, b is independently 0 or1, respectively, and D is independently represented by the structure of the following general formula (III):

wherein i is each independently 0 or 1;

j is each independently 0,1, 2,3, 4,5 or 6;

a is each independently S, O or N-Ra, wherein Ra is hydrogen, alkyl, alkenyl or alkoxy;

each B is independently alkyl, alkenyl, -O-alkyl, -alkyl-O, -S-alkyl, -alkyl-S, -aryl, heteroaryl, heterocyclyl or peptide, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, - -alkyl-aryl, - -alkyl-heteroaryl, - -alkyl-heterocyclyl, - - -O- -R₄- - -O-alkyl-R₄，--C(O)-R₄，--C(O)-O-R₄，--S-R₄，--S(O)₂-R₄，--NHR₄- - -NH-alkyl-R₄Halogen, - -CN, - -NO₂and-SH, wherein R₄Is alkyl, alkenyl, -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl.

In another aspect, the present invention provides a compound having the structure of the following general formula (Ib):

TM-L-AM (Ib)

wherein TM is a targeting moiety, L is a linker, and AM is an activating moiety represented by the structure of the following general formula (IV):

wherein,is the point to be connected to the connector;

wherein V is-NR₆R₇Wherein R is₆And R₇Each independently is hydrogen, alkyl, alkenyl, alkoxy, alkylamino, dialkylamino, alkylthio, arylthio, - - -alkyl-hydroxy, - -alkyl-C (O) -O- -R₉- - (O) -R- -alkyl-C₉or-alkyl-O-C (O) -R₉Wherein R is₉Is hydrogen, alkyl, alkenyl, halogen or haloalkyl;

R₁₀and R₁₁Independently hydrogen, alkyl, alkenyl, aryl, haloalkyl, heteroaryl, heterocyclyl or cycloalkyl, each of which is optionally substituted with one or more substituents selected from: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, halogen, - -N (R)₅)₂-alkoxy-alkyl, -alkoxy-alkenyl, -C (O)-alkyl, -C (O) -O-alkyl, -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl, and- -CO-heteroaryl, wherein R is₅Each independently is hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkyl-heteroaryl; TM and L are defined as described above and below.

In some embodiments, the TM specifically binds to a tumor cell or preferentially binds to a tumor cell relative to a non-tumor cell. In some embodiments, the tumor cell is a cancer cell, a sarcoma cell, a lymphoma cell, a myeloma cell, or a central nervous system cancer cell.

In some embodiments, the TM specifically binds to a tumor antigen on a tumor cell or preferentially binds to a tumor antigen on a tumor cell relative to a non-tumor antigen. In some embodiments, the tumor antigen is selected from the group consisting of: CD2, CD19, CD20, CD22, CD27, CD33, CD37, CD38, CD40, CD44, CD47, CD52, CD56, CD70, CD79 and CD 137.

In some embodiments, the tumor antigen is selected from the group consisting of: 4-1BB,5T4, AGS-5, AGS-16, angiopoietin 2, B7.1, B7.2, B7DC, B7H1, B7H2, B7H3, BT-062, BTLA, CAIX, carcinoembryonic antigen, CTLA4, Cripto, ED-B, ErbB1, ErbB2, ErbB3, ErbB4, EGFL 4, EpCAM, EphA 4, EphB 4, FAP, fibronectin, folate receptor, ganglioside GM 4, GD 4, glucocorticoid-induced tumor necrosis factor receptor (GIpA TR), gp100, g3672, GPNMB, ICOS, IGF 14, integrin α v, β v β, KIR, TAG-3, Lewis Y, mesothelin, METc-MN, MUC-5, MUC-72, MUSCH 72, TROC 1, TROC 72, TROC 4, TRAC 4, CROCR 4, TRACK 4, TRACP 4, TRACK 4, CROCH-4, CROCK-4, CROCH-4, CROCK, VEGFR-2, VEGFR-3 and variants thereof.

In some embodiments, the TM comprises an immunoglobulin, a protein, a peptide, a small molecule, a nanoparticle, or a nucleic acid.

In some embodiments, the TM comprises an antibody or functional fragment thereof. In some embodiments, the antibody is selected from the group consisting of: rituximab, herceptin, trastuzumab, erbitux, verterby, alemtb, arzerra, (ofatumumab), Benlysta (belimumab), Yervoy, Perjeta (pertuzumab), Tremelimumab, Nivolumab, Dacetuzumab, Urelumab, MPDL3280A, Lambrolizumab and blinatumumab.

In some embodiments, the TM comprises a Fab, Fab ', F (ab')2, single domain antibody, T and Abs dimer, Fv, scFv, dsFv, ds-scFv, Fd, linear antibody, minibody, diabody, bispecific antibody fragment, bibody, tribody, sc-diabody, kappa (. lamda.) body, BiTE, DVD-Ig, SIP, SMIP, DART, or antibody analog containing one or more CDRs.

In some embodiments, the TM comprises an ATWLPPR polypeptide of VEGFR, a thrombospondin-1 mimetic, an CDCRGDCFCG (cyclic) polypeptide, an SCH221153 fragment, an NCNGRC (cyclic) polypeptide, a CTTHWGFTLC polypeptide, a CGNKRTRGC polypeptide (LyP-1), octreotide, vapreotide, lanreotide, a C-3940 polypeptide, dabigatran, leprosy, norrad, or cetrorelix.

In some embodiments, the TM comprises folic acid or a derivative thereof.

In some embodiments, the TM comprises an extracellular domain (ECD) or a soluble form of PD-1, CTLA4, BTLA, KIR, TIM3,4-1BB, LAG3, a full-length partial surface ligand amphiregulin, betacellulin, EGF, ephrin, epigen, epiregulin, IGF, neuregulin, TGF, TRAIL, or VEGF.

In some embodiments, the TM comprises a nanoparticle.

In some embodiments, the TM comprises an aptamer.

In some embodiments, the TM comprises: rituximab, herceptin (trastuzumab), erbitux (cetuximab), victoria (panitumumab), arzerra (ofatumumab), Benlysta (belimumab), Yervoy (ipilimumab), Perjeta (pertuzumab), Tremelimumab, Nivolumab, Dacetuzumab, Urelumab, MPDL3280A, Lambrolizumab, blinatumumab, aldesleukin (aldesleukin), aemtuzumab, arivulvonic acid (alitetinin), allopurinol, altretamine (altretamine), amifostine (amifosiltine), anastrozole (anastrozole), arsenic, asparaginase, liase, liotin, betahistidine capsule (beaxarotene), saxarotene (amitripteryne), intravenous bletacrine (bletacrine), bletacrine (bletacrine, bletacrine (bletacrine), bletacrine (bletacrine, bletacrine (bletacrine), bletacrine (oral administration of bletacrine, bletacrine (bletacrine, caprine, capram, bletacrine, oral administration of oral, Cytarabine liposome, dacarbazine (dacarbazine), dactinomycin, alfa bepotin (dabepoetin alfa), daunorubicin hydrochloride liposome, daunorubicin hydrochloride, daunorubicin, dnitukin diftitox, dexrazoxane (dexrazoxane), docetaxel, doxorubicin liposome, dromostone propionate (domostanolone propinate), eliott's B solution, epirubicin, eoetin alfa estramustine, etoposide phosphate, Poposide (VP-16), exemestane, flastim, fluorouracil (intra-arterial use), fludarabine (fludarabine), fluorouracil (5-FU), fulvestrant (fulvestrant), gemtuzumab geuzumab, sertraline, hydroxyurea acetate, ibrutinib (ibrutin), interferon alfa 2, and isosfamide, Irinotecan, trovazole, calcium folinate, levamisole (levamisole), lomustine (CCNU), dichloromethyldiethylamine (nitrogen mustard), megestrol acetate, melphalan (L-PAM), mercaptopurine (6-MP), mesna, methotrexate, methoxsalen, mitomycin C, mitotane, mitoxantrone, phenthoquinone, nandrolone propionate, nfetumomab, LOddC, orelevin, oxaliplatin, paclitaxel, pamidronate, pegademase, pemetrexed (Pegaspargase), PEGADEmase (Pegaspargase), PEGASPARGARTASE (Pegfilgrastim), pentostatin, pipobroman, plicamycin, mithramycin, phenothrin, procarbazine, apine (quinacrine), labirinase, sargaseitine, tenipostine, tenipodine, teniposide (LDT-6-D), Teniposide (TG), tetrodotimoside, Tetrodotoxin (TG), tetrodotoxin (T-T), tetrodotoxin (T-D), D-D, thiotepa, topotecan, toremifene (toremifene), Tositumomab (Tositumomab), tretinoin (ATRA), uramustine (Uracil Mustard), valrubicin, pantocitabine (valtocitabine, monovLDC), vinblastine, vinorelbine, zoledronic acid (zoledronate), and mixtures thereof.

In some embodiments, D in the linker moiety ("L") is selected from the following structures of formula (V) to formula (VII):

A. b, i and j are as defined above.

In some embodiments, the present invention provides a pharmaceutical composition comprising a compound provided herein, or a pharmaceutically acceptable salt thereof, and/or one or more pharmaceutically acceptable carriers.

In some embodiments, the pharmaceutical composition further comprises an additional therapeutic agent. In some embodiments, the additional therapeutic agent is an anti-cancer agent. In some embodiments, the additional therapeutic agent is an antimetabolite, an inhibitor of topoisomerase I and topoisomerase II, an alkylating agent, a microtubule inhibitor, an anti-androgenic agent, a GNRh modulator, or a mixture thereof. In some embodiments, the additional therapeutic agent is selected from: tamoxifen, raloxifene, anastrozole, exemestane, letrozole, imatanib, paclitaxel, cyclophosphamide, lovastatin, minosine, gemcitabine, cytarabine, 5-fluorouracil, methotrexate, docetaxel, goserelin, vincristine, vinblastine, thiabendazole, teniposide, etoposide, gemcitabine, epothilone, vinorelbine, daunorubicin, mitoxantrone, idarubicin, dactinomycin, vinorelbine, camptothecin, daunorubicin, mitoxantrone, acridine, doxorubicin, epirubicin or oxyrubicin.

In yet another aspect, the invention provides a method of inhibiting tumor cell proliferation comprising administering a compound of the invention to the tumor cell.

In some embodiments, the present invention provides a method of treating a disease in a subject, comprising administering to the subject a compound of the present invention. In some embodiments, the disease is cancer/tumor. In some embodiments, the disease is a cancer selected from the group consisting of: gastric, colon, rectal, liver, pancreatic, lung, breast, cervical, uterine corpus, ovarian, testicular, bladder, kidney, brain/central nervous system, head and neck, throat, hodgkin's disease, non-hodgkin's lymphoma, multiple myeloma, leukemia, melanoma, non-melanoma skin cancer, acute lymphocytic leukemia, acute myelogenous leukemia, ewing's sarcoma, small cell lung cancer, chorioepithelioma, rhabdomyosarcoma, wilms tumor, neuroblastoma, hairy cell leukemia, oropharyngeal, esophageal, laryngeal, or lymphoma.

In some embodiments, the disease is a tumor. In some embodiments, the disease comprises abnormal cell proliferation. In some embodiments, the abnormal cell proliferation comprises a precancerous lesion. In some embodiments, the abnormally proliferating cell is a cancer cell.

Drawings

The novel features believed characteristic of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:

FIG. 1 shows the properties of the coupled compounds. Binding of trastuzumab MC-vc-resiquimod and trastuzumab-resiquimod conjugates to Her 2-expressing L cells was compared to unconjugated trastuzumab (as reference), control human IgG, and unrelated human IgG1 antibodies as determined by FACS analysis.

FIG. 2 shows in vitro Antibody Dependent Cellular Cytotoxicity (ADCC) assays for trastuzumab, trastuzumab MC-vc-Toll-like receptor ligand (TLRL), and trastuzumab MC-Toll-like receptor ligand (TLRL) conjugates. PBMCs (effector cells) were added to 96-well plates (target/effector ratio 1/60) containing different concentrations of control human IgG1, trastuzumab or trastuzumab MC-vc-TLRL and trastuzumab MC-TLRL conjugates for 17 hours together with SKBR3 cells (target cells). All experiments were performed in duplicate. The analysis was based on a negative SKBR3 population assay. Dead cells were stained with LDH.

FIG. 3A shows the percentage of DCs before and after enrichment. The numbers in the upper two panels represent the percentage of DC (HLA-DR + Lin-) in total cells before and after lineage depletion. The numbers in the lower panel represent the percentage of mdcs (CD 11C + CD 123-) and pdcs (CD 123+ CD 11C-) in total DCs before and after lineage depletion. FIGS. 3B to 3D show cytokine production analysis of purified human DCs. Purified human DCs were seeded in 96-well plates and cultured directly with allogeneic untreated (medium) or treated different concentrations of TLRL or trastuzumab MC-vc-TLRL and trastuzumab MC-TLRL in an incubator at 37 ℃ for 20 to 22 hours. In another experiment, increasing concentrations of cetuximab MC-vc-TLRL and cetuximab MC-TLRL were provided for treatment with human DC. Supernatants were collected and analyzed by ELISA for human IFN-. alpha.IL-6, IL-12(p70) and TNF-. alpha.. Data are expressed as mean ± SD of triplicate cultures and data represent independent experiments from two of three healthy donors.

FIGS. 4A-4C show the therapeutic effect of trastuzumab MC-vc-TLRL and trastuzumab MC-TLRL in a PDX gastric tumor model. Female BALB/c nude mice (nu/nu) 6 to 8 weeks old with patient derived gastric tumors were treated by intravenous injection of 10mg/kg trastuzumab MC-vc-TLRL, trastuzumab MC-TLRL or unconjugated trastuzumab or saline (12 mice per group). Treatment was performed weekly for a 45 day period. When the tumor reached an average size of 170mm³Treatment is initiated. FIG. 4A: data represent mean tumor volume (mean ± SD). When the tumor reached a size of 2000mm³The tumor growth curve stopped. FIG. 4B: changes in body weight of mice during and after treatment. No detectable weight loss was observed. FIG. 4C: survival curves for treatment and control groups; the lifespan of mice receiving trastuzumab MC-vc-TLRL or unconjugated trastuzumab was greatly increased.

FIGS. 5A-5B show the efficacy of cetuximab MC-vc-TLRL in a nude mouse/H1650 human lung cancer model. 6-to 8-week-old female BALB/c nude mice (nu/nu) subcutaneously with H1650 lung cancer cells were treated by intravenous injection of 10mg/kg cetuximab MC-vc-TLRL or unconjugated cetuximab or saline (12 mice per group). Treatment was performed weekly for a 45 day period. When the tumor reached an average size of 170mm³Treatment is initiated. Data represent mean tumor volume (mean ± SD). When the tumor reached a size of 2000mm³The tumor growth curve stopped. FIG. 5A: efficacy of cetuximab MC-vc-TLRL in a human lung cancer model. FIG. 5B: changes in body weight of mice during and after treatment. No detectable weight loss was observed.

Detailed Description

Various aspects of the invention are described below in conjunction with exemplary applications for illustration. It should be understood that numerous specific details, relationships, and methods are set forth to provide a full understanding of the invention. One skilled in the relevant art will readily recognize, however, that the invention may be practiced without one or more of the specific details, or with other methods. The invention is not limited by the illustrated ordering of operations or events, as some operations may occur in different orders and/or concurrently with other operations or events.

Further, not all illustrated acts or events are required to be performed in accordance with the methodologies of the present invention.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The articles "a," "an," and "the," as used herein without specifying a particular number, are intended to include the plural forms as well, unless expressly stated otherwise. Furthermore, the terms "comprising," including, "" having, "" has, "" with, "or variations thereof as used in the detailed description and/or the claims are intended to be inclusive in a manner similar to the term" comprising.

The term "about" or "approximately" means that the particular value determined by one of ordinary skill in the art is within an acceptable error range that depends in part on how the value is measured or determined, i.e., the limitations of the measurement system. For example, "about" can mean within 1 standard deviation or more than one standard deviation per operation in the art. Alternatively, "about" may mean a range of up to 20% of a given value, preferably up to 10% of a given value, more preferably up to 5% of a given value and more preferably up to 1% of a given value. Alternatively, especially for biological systems or processes, the term may mean within an order of magnitude of a certain value, preferably within 5 times a certain value, more preferably within 2 times a certain value. Where particular values are described in the application and claims, unless otherwise stated, it should be assumed that the term "about" means within an acceptable error range for the particular value.

Definitions and abbreviations

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Generally, the nomenclature used herein and the laboratory procedures in cell culture, molecular genetics, organic chemistry, and nucleic acid chemistry, and hybridization are those well known and commonly employed in the art. Standard techniques are used for nucleic acid and peptide synthesis. The techniques and procedures are generally performed according to conventional methods in the art and various conventional references, which are provided herein. The nomenclature used herein and the laboratory procedures in analytical chemistry and organic synthesis described below are those well known and commonly employed in the art. Standard techniques or modifications thereof are used for chemical synthesis and chemical analysis.

Unless otherwise specified, the term "alkyl" by itself or as part of another substituent means containing the indicated number of carbon atoms (i.e., C)₁-C₁₀Refers to 1 to 10 carbon atoms) or combinations thereof, which may be fully saturated, mono-unsaturated or poly-unsaturated and may include divalent and polyvalent radicals. Examples of saturated hydrocarbon radicals include, but are not limited to, radicals such as methyl, ethyl, n-propyl, isopropyl, n-butyl, tert-butyl, isobutyl, sec-butyl, cyclohexyl, (cyclohexyl) methyl, cyclopropylmethyl, homologs or isomers thereof, e.g., n-pentyl, n-hexyl, n-heptyl, n-octyl, and the like. Unsaturated alkyl is alkyl having one or more double or triple bonds. Examples of unsaturated alkyl groups include, but are not limited to: vinyl, 2-propenyl, crotyl, 2-isopentenyl, 2- (butadienyl), 2, 4-pentadienyl, 3- (1, 4-pentadienyl), ethynyl, 1-and 3-propynyl,3-butynyl and higher homologs and isomers. Unless otherwise indicated, the term "alkyl" is also intended to include those alkyl derivatives defined in more detail below, for example, "heteroalkyl". The alkyl group defined as a hydrocarbyl group is referred to as a "homoalkyl".

The term "alkene" by itself or as part of another substituent refers to a divalent radical derived from an alkane, such as, but not limited to: -CH₂CH₂CH₂CH₂And also includes those groups described below as "heteroalkenes". In general, alkyl (or alkene) groups can have 1 to 24 carbon atoms, with those groups having 10 or fewer carbon atoms being preferred in the present invention. "lower alkyl" or "lower olefin" is a shorter chain alkyl or olefin group, typically having 8 or fewer carbon atoms.

The terms "alkoxy", "alkylamino" and "alkylthio" (or thioalkoxy) are used in their conventional sense and refer to those alkyl groups attached to the remainder of the molecule through an oxygen atom, an amino group, or a sulfur atom, respectively.

Unless otherwise specified, the term "heteroalkyl," by itself or in combination with another term, refers to a stable straight or branched chain or cyclic hydrocarbon radical, or combinations thereof, consisting of the stated number of carbon atoms and at least one heteroatom selected from O, N, Si and S, and wherein the nitrogen and sulfur atoms may be optionally oxidized and the nitrogen heteroatom may be optionally quaternized. The heteroatoms O, N and S and Si can be located at any internal position of the heteroalkyl group or at the position where the alkyl group is attached to the remainder of the molecule. Examples include, but are not limited to: -CH₂-CH₂-O-CH₃,-CH₂-CH₂-NH-CH₃,-CH₂-CH₂-N(CH₃)-CH₃,-CH₂-S-CH₂-CH₃,-CH₂-CH₂,-S(O)-CH₃,-CH₂-CH₂-S(O)₂-CH₃,-CH=CH-O-CH₃,-Si(CH₃)₃,-CH₂-CH=N-OCH₃and-CH = CH-N (CH)₃)-CH₃. Up to two heteroatoms may be consecutive, e.g., -CH₂-NH-OCH₃and-CH₂-O-Si(CH₃)₃. Similarly, the term "heteroalkene" by itself or as part of another substituent refers to a divalent radical derived from a heteroalkyl group, such as, but not limited to: -CH₂-CH₂-S-CH₂-CH₂-and-CH₂-S-CH₂-CH₂-NH-CH₂-. For heteroalkenyl groups, a heteroatom can also occupy one or both ends of a chain end (e.g., an alkene oxy, an alkene dioxy, an alkene amino, an alkene diamino, and the like). Furthermore, for linking groups of alkenes and heteroalkenes, the orientation in the formula of the linking group as written does not imply the orientation of the linking group. For example, of the formula-C (O)₂R' -represents-C (O)₂R '-and-R' C (O)₂-。

In general, "acyl substituent" is also selected from the groups listed above. The term "acyl substituent" as used herein refers to a group attached to the polycyclic core of a compound of the invention and which satisfies the valence of the carbonyl carbon attached, directly or indirectly, to the polycyclic core of a compound of the invention.

Unless otherwise indicated, the terms "cycloalkyl" and "heterocycloalkyl", by themselves or in combination with other terms, represent cyclic forms of "alkyl" and "heteroalkyl", respectively. Further, for heterocycloalkyl, a heteroatom may occupy the position where the heterocycle is attached to the remainder of the molecule. Examples of cycloalkyl groups include, but are not limited to: cyclopentyl, cyclohexyl, 1-cyclohexenyl, 3-cyclohexenyl, cycloheptyl, and the like. Examples of heterocycloalkyl groups include, but are not limited to: 1- (1,2,5, 6-tetrahydropyridinyl), 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, 4-morpholinyl, 3-morpholinyl, tetrahydrofuran-2-yl, tetrahydrofuran-3-yl, tetrahydrothiophen-2-yl, tetrahydrothiophen-3-yl, 1-piperazinyl, 2-piperazinyl, and the like.

Unless otherwise indicated, the terms "halo" or "halogen"by itself or as part of another substituent means a fluorine, chlorine, bromine or iodine atom. Further, terms such as "haloalkyl" are intended to include monohaloalkyl and polyhaloalkyl. For example, the term "halo (C)₁-C₄) Alkyl "is intended to include, but is not limited to: trifluoromethyl, 2,2, 2-trifluoroethyl, 4-chlorobutyl, 3-bromopropyl, and the like.

The term "haloalkyl" as used herein, refers to an alkyl group, as defined herein, substituted with one or more halo groups, as defined herein. The haloalkyl group may preferably be a monohaloalkyl group, a dihaloalkyl group or a polyhaloalkyl group (including perhaloalkyl groups). The monohaloalkyl group may have one iodo, bromo, chloro or fluoro in the alkyl group. Dihaloalkyl and polyhaloalkyl groups may have two or more of the same halogen atom or a combination of different halogen groups in the alkyl group. Preferably, the polyhaloalkyl group contains up to 12, 10 or 8, or 6, or 4, or 3, or2 halogen groups. Non-limiting examples of haloalkyl groups include fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, dichloromethyl, trichloromethyl, pentafluoroethyl, heptafluoropropyl, difluorochloromethyl, dichlorofluoromethyl, difluoroethyl, difluoropropyl, dichloroethyl and dichloropropyl. Perhaloalkyl refers to alkyl groups in which all hydrogen atoms are substituted with halogen atoms.

The term "heteroaryl" as used herein refers to a 5-to 14-membered monocyclic or bicyclic or fused polycyclic ring system having 1 to 8 heteroatoms selected from N, O, S or Se. Preferably, the heteroaryl is a 5-to 10-membered ring system. Typical heteroaryl groups include 2-thienyl or 3-thienyl, 2-furyl or 3-furyl, 2-pyrrolyl or 3-pyrrolyl, 2-imidazolyl, 4-imidazolyl or 5-imidazolyl, 3-pyrazolyl, 4-pyrazolyl or 5-pyrazolyl, 2-thiazolyl, 4-thiazolyl or 5-thiazolyl, 3-isothiazolyl, 4-isothiazolyl or 5-isothiazolyl, 2-oxazolyl, 4-oxazolyl or 5-oxazolyl, 3-isoxazolyl, 4-isoxazolyl or 5-isoxazolyl, 3-1,2, 4-triazolyl or 5-1,2, 4-triazolyl, 4-1,2, 3-triazolyl or 5-1,2, 3-triazolyl, tetrazolyl, 2-pyridyl, 3-pyridyl or 4-pyridyl, 3-pyridazinyl or 4-pyridazinyl, 3-pyrazinyl, 4-pyrazinyl or 5-pyrazinyl, 2-pyrimidinyl, 4-pyrimidinyl or 5-pyrimidinyl.

The term "heteroaryl" also refers to a group in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocycloalkyl rings, where a radical or point of attachment is on the heteroaromatic ring. Non-limiting examples include, but are not limited to: 1-indolizinyl, 2-indolizinyl, 3-indolizinyl, 5-indolizinyl, 6-indolizinyl, 7-indolizinyl or 8-indolizinyl, 1-isoindolyl, 3-isoindolyl, 4-isoindolyl, 5-isoindolyl, 6-isoindolyl or 7-isoindolyl, 2-indolyl, 3-indolyl, 4-indolyl, 5-indolyl, 6-indolyl or 7-indolyl, 2-indazolyl, 3-indazolyl, 4-indazolyl, 5-indazolyl, 6-indazolyl or 7-indolyl, 6-indazolyl, 7-purinyl or 8-purinyl, 1-quinolizinyl, 2-quinolizinyl, 3-quinolizinyl, 4-quinolizinyl, 6-quinolizinyl, 7-quinolizinyl, 8-quinolizinyl or 9-quinolizinyl, 2-quinolyl, 3-quinolyl, 4-quinolyl, 5-quinolyl, 6-quinolyl, 7-quinolyl or 8-quinolyl, 1-isoquinolyl, 3-isoquinolyl, 4-isoquinolyl, 5-isoquinolyl, 6-isoquinolyl, 7-isoquinolyl or 8-isoquinolyl, 1-2, 3-naphthyridinyl, 4-2, 3-naphthyridinyl, 5-2, 3-naphthyridinyl, 6-2, 3-naphthyridinyl, 7-2, 3-naphthyridinyl or 8-2, 3-naphthyridinyl, 2-1, 5-naphthyridinyl, 3-1, 5-naphthyridinyl, 4-1, 5-naphthyridinyl, 5-1, 5-naphthyridinyl or 6-1, 5-naphthyridinyl, 2-quinazolinyl, 3-quinazolinyl, 5-quinazolinyl, 6-quinazolinyl, 7-quinazolinyl or 8-quinazolinyl, 3-cinnolinyl, 4-cinnolinyl, 5-cinnolinyl, 6-cinnolinyl, 7-cinnolinyl or 8-cinnolinyl, 2-pteridinyl, 4-pteridinyl, 6-pteridinyl or 7-pteridinyl, 1-4aH carbazolyl, 2-4aH carbazolyl, 3-4aH carbazolyl, 4-4aH carbazolyl, 5-4aH carbazolyl group, 6-4aH carbazolyl group, 7-4aH carbazolyl group or 8-4aH carbazolyl group, 1-carbazolyl group, 2-carbazolyl group, 3-carbazolyl group, 4-carbazolyl group, 5-carbazolyl group, 6-carbazolyl group, 7-carbazolyl group or 8-carbazolyl group, 1-carbolinyl group, 3-carbolinyl group, 4-carbolinyl group, 5-carbolinyl group, 6-carbolinyl group, 7-carbolinyl group, 8-carbolinyl group or 9-carbolinyl group, 1-phenanthridinyl group, 2-phenanthridinyl group, 3-phenanthridinyl group, 4-phenanthridinyl group, 6-phenanthridinyl group, 7-phenanthridinyl group, 8-phenanthridinyl group, 9-phenanthridinyl group or 10-phenanthridinyl group, 1-acridinyl group, 2-acridinyl group, 3-acridinyl group, 4-acridinyl, 5-acridinyl, 6-acridinyl, 7-acridinyl, 8-acridinyl or 9-acridinyl, 1-rylnaphthyridinyl, 2-rylenandinophenyl, 4-rylnaphthyridinyl, 5-rylenandinophenyl, 6-rylenandinophenyl, 7-rylenandinophenyl, 8-or 9-rylenandinophenyl (perimidyl), 2-phenanthrolinyl, 3-phenanthrolinyl, 4-phenanthrolinyl, 5-phenanthrolinyl, 6-phenanthrolinyl, 8-phenanthrolinyl, 9-phenanthrolinyl or 10-phenanthrolinyl, 1-phenazinyl, 2-phenazinyl, 3-phenazinyl, 4-phenazinyl, 6-phenazinyl, 7-phenazinyl, 8-phenazinyl or 9-phenazinyl, 1-thienyloxazinyl, 2-thienyloxazinyl, 3-thienyloxazinyl, 4-thienyloxazinyl, 6-thienyloxazinyl, 7-thienyloxazinyl, 8-thienyloxazinyl, 9-thienyloxazinyl or 10-thienyloxazinyl, 1-phenoxazinyl, 2-phenoxazinyl, 3-phenoxazinyl, 4-phenoxazinyl, 6-phenoxazinyl, 7-phenoxazinyl, 8-phenoxazinyl, 9-phenoxazinyl or 10-phenoxazinyl, l-benzisoquinolinyl, 3-benzisoquinolinyl, 4-benzisoquinolinyl, 5-benzisoquinolinyl, 6-benzisoquinolinyl, 7-benzisoquinolinyl, 8-benzisoquinolinyl, 9-benzisoquinolinyl or 10-benzisoquinolinyl, 2-thieno [2,3-b ] furyl, 3-thieno [2,3-b ] furyl, 4-thieno [2,3-b ] furyl or 5-thieno [2,3-b ] furyl, 2-7H-pyrazino [2,3-c ] carbazolyl, 3-7H-pyrazino [2,3-c ] carbazolyl, 5-7H-pyrazino [2,3-c ] carbazolyl, 6-7H-pyrazino [2,3-c ] carbazolyl, 7-7H-pyrazino [2,3-c ] carbazolyl, 8-7H-pyrazino [2,3-c ] carbazolyl, 9-7H-pyrazino [2,3-c ] carbazolyl, 10-7H-pyrazino [2,3-c ] carbazolyl or 11-7H-pyrazino [2,3-c ] carbazolyl, 2-2H-furo [3,2-b ] -pyranyl, 3-2H-furo [3,2-b ] -pyranyl, 5-2H-furo [3,2-b ] -pyranyl, 6-2H-furo [3,2-b ] -pyranyl or 7-2H-furo [3,2-b ] -pyranyl, 2-5H-pyrido [2,3-d ] -o-oxazinyl, 3-5H-pyrido [2,3-d ] -o-oxazinyl, 4-5H-pyrido [2,3-d ] -o-oxazinyl, 5-5H-pyrido [2,3-d ] -o-oxazinyl, 7-5H-pyrido [2,3-d ] -o-oxazinyl or 8-5H-pyrido [2,3-d ] -o-oxazinyl, 1-1H-pyrazolo [4,3-d ] -oxazolyl, 3-1H-pyrazolo [4,3-d ] -oxazolyl or 5-1H-pyrazolo [4,3-d ] -oxazolyl, 2-4H-imidazo [4,5-d ] thiazolyl, 4-4H-imidazo [4,5-d ] thiazolyl or 5-4H-imidazo [4,5-d ] thiazolyl, 3-pyrazino [2,3-d ] pyridazinyl, 5-pyrazino [2,3-d ] pyridazinyl or 8-pyrazino [2,3-d ] pyridazinyl, 2-imidazo [2,1-b ] thiazolyl, 3-imidazo [2,1-b ] thiazolyl, 5-imidazo [2,1-b ] thiazolyl or 6-imidazo [2,1-b ] thiazolyl, 1-furo [3,4-c ] cinnolinyl, 3-furo [3,4-c ] cinnolinyl, 6-furo [3,4-c ] cinnolinyl, 7-furo [3,4-c ] cinnolinyl, 8-furo [3,4-c ] cinnolinyl or 9-furo [3,4-c ] cinnolinyl, 1-4H-pyrido [2,3-c ] carbazolyl, 2-4H-pyrido [2,3-c ] carbazolyl, 3-4H-pyrido [2,3-c ] carbazolyl, 4-4H-pyrido [2,3-c ] carbazolyl, 5-4H-pyrido [2,3-c ] carbazolyl, 6-4H-pyrido [2,3-c ] carbazolyl, 8-4H-pyrido [2,3-c ] carbazolyl, 9-4H-pyrido [2,3-c ] carbazolyl, 10-4H-pyrido [2,3-c ] carbazolyl or 11-4H-pyrido [2,3-c ] carbazolyl, 2-imidazo [1,2-b ] [1,2,4] triazinyl, 3-imidazo [1,2-b ] [1,2,4] triazinyl, 6-imidazo [1,2-b ] [1,2,4] triazinyl or 7-imidazo [1,2-b ] [1,2,4] triazinyl, 7-benzo [ b ] thienyl, 2-benzoxazolyl, 4-benzoxazolyl, 5-benzoxazolyl, 6-benzoxazolyl or 7-benzoxazolyl, 2-benzimidazolyl, 4-benzimidazolyl, 5-benzimidazolyl, 6-benzimidazolyl or 7-benzimidazolyl, 2-benzothiazolyl, 4-benzothiazolyl, 5-benzothiazolyl, 6-benzothiazolyl or 7-benzothiazolyl, 1-benzoxanyl, 2-benzoxanyl, 4-benzoxanyl, 5-benzoxanyl, 6-benzoxanyl, 7-benzoxanyl, 8-benzoxanyl or 9-benzoxanyl (benzoxapinyl), 2-benzooxazinyl, 4-benzooxazinyl, 5-benzooxazinyl, 6-benzooxazinyl, 7-benzooxazinyl or 8-benzooxazinyl, 1-1H-pyrrolo [1,2-b ] [2] benzoxanyl, 2-1H-pyrrolo [1,2-b ] [2] benzoxanyl, 3-1H-pyrrolo [1,2-b ] [2] benzoxanyl, 5-1H-pyrrolo [1,2-b ] [2] benzazepinyl, 6-1H-pyrrolo [1,2-b ] [2] benzazepinyl, 7-1H-pyrrolo [1,2-b ] [2] benzazepinyl, 8-1H-pyrrolo [1,2-b ] [2] benzazepinyl, 9-1H-pyrrolo [1,2-b ] [2] benzazepinyl, 10-1H-pyrrolo [1,2-b ] [2] benzazepinyl, or 11-1H-pyrrolo [1,2-b ] [2] benzazepinyl (benzazepinyl). Typical fused heteroaryl groups include, but are not limited to: 2-quinolyl, 3-quinolyl, 4-quinolyl, 5-quinolyl, 6-quinolyl, 7-quinolyl or 8-quinolyl, 1-isoquinolyl, 3-isoquinolyl, 4-isoquinolyl, 5-isoquinolyl, 6-isoquinolyl, 7-isoquinolyl or 8-isoquinolyl, 2-indolyl, 3-indolyl, 4-indolyl, 5-indolyl, 6-indolyl or 7-indolyl, 2-benzo [ b ] thienyl, 3-benzo [ b ] thienyl, 4-benzo [ b ] thienyl, 5-benzo [ b ] thienyl, 6-benzo [ b ] thienyl or 7-benzo [ b ] thienyl, 2-benzoxazolyl, 4-benzoxazolyl, 5-benzoxazolyl, 6-benzoxazolyl or 7-benzoxazolyl, 2-benzimidazolyl, 4-benzimidazolyl, 5-benzimidazolyl, 6-benzimidazolyl or 7-benzimidazolyl, 2-benzothiazolyl, 4-benzothiazolyl, 5-benzothiazolyl, 6-benzothiazolyl or 7-benzothiazolyl.

The term "heterocyclyl" or "heterocycle" as used herein refers to an optionally substituted fully saturated or unsaturated, aromatic or non-aromatic cyclic group, for example, which is a 4-to 7-membered monocyclic, 7-to 12-membered bicyclic, or 10-to 15-membered tricyclic ring system having at least one heteroatom in at least one carbon atom-containing ring. Each ring of the heterocyclic group containing a heteroatom may have 1,2 or 3 heteroatoms selected from nitrogen atoms, oxygen atoms and sulfur atoms, wherein the nitrogen atoms and sulfur atoms may also be optionally oxidized. The heterocyclic group may be attached at a heteroatom or carbon atom.

Exemplary monocyclic heterocyclic groups include: pyrrolidinyl, pyrrolyl, pyrazolyl, oxetanyl, pyrazolinyl, imidazolyl, imidazolinyl, imidazolidinyl, triazolyl, oxazolyl, oxazolidinyl, isoxazolinyl, isoxazolyl, thiazolyl, thiadiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, furyl, tetrahydrofuryl, thienyl, oxadiazolyl, piperidinyl, piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, 2-oxoazepinyl (2-oxoazepinyl), azepinyl (azepinyl), 4-piperidinonyl, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, tetrahydropyranyl, morpholinyl, thiomorpholinyl sulfoxide, thiomorpholinyl sulfone, 1, 3-dioxolane and tetrahydro-1, 1-dioxothienyl, 1, 4-trioxo-1, 2, 5-thiadiazolidin-2-yl, and the like.

Exemplary bicyclic heterocyclic groups include: indolyl, indolinyl, benzothiazolyl, benzoxazinyl, benzoxazolyl, benzothienyl, benzothiazolyl, quinuclidinyl, quinolinyl, tetrahydroquinolinyl, decahydroquinolinyl, isoquinolinyl, tetrahydroisoquinolinyl, decahydroisoquinolinyl, benzimidazolyl, benzopyranyl, indolizinyl, benzofuranyl, chromonyl, coumarinyl, benzopyranyl, cinnolinyl, quinoxalinyl, indazolyl, pyrrolopyridyl, furopyridinyl (e.g., furo [2,3-c ] pyridinyl, furo [3,2-b ] pyridinyl or furo [2,3-b ] pyridinyl), dihydroisoindolyl, 1, 3-dioxo-1, 3-dihydroisoindol-2-yl, dihydroquinazolinyl (e.g., 3, 4-dihydro-4-oxo-quinazolinyl), 2, 3-naphthyridinyl, and the like.

Exemplary tricyclic heterocyclic groups include: carbazolyl, dibenzoazapinyl, dithienoazepinyl, benzindolyl, phenanthrolinyl, acridinyl, phenanthridinyl, phenoxazinyl, phenothiazinyl, xanthenyl, carbolinyl, and the like.

The term "heterocyclyl" further refers to a heterocyclic group as defined above substituted with 1,2 or 3 substituents selected from the group consisting of:

(a) an alkyl group;

(b) hydroxyl (or protected hydroxyl);

(c) halogen;

(d) oxygen, i.e., = O;

(e) amino, alkylamino or dialkylamino;

(f) an alkoxy group;

(g) a cycloalkyl group;

(h) a carboxyl group;

(i) heterocyclyloxy, wherein heterocyclyloxy refers to a heterocyclic group connected by an oxygen bridge;

(j) alkyl-O-C (O) -;

(k) a mercapto group;

(l) A nitro group;

(m) cyano;

(n) a sulfamoyl or sulfonamido group;

(o) an aryl group;

(p) alkyl-C (O) -O- -;

(q) aryl-C (O) -O- -;

(r) aryl-S-;

(s) aryloxy;

(t) alkyl-S- -;

(u) formyl, i.e., HC (O) -;

(v) a carbamoyl group;

(w) aryl-alkyl-; and

(x) Aryl substituted with alkyl, cycloalkyl, alkoxy, hydroxy, amino, alkyl-C (O) -NH-, alkylamino, dialkylamino or halogen.

The term "alkenyl" as used herein refers to a straight or branched chain hydrocarbon group having 2 to 20 carbon atoms and containing at least one double bond. The alkenyl group preferably has about 2 to 8 carbon atoms.

Unless otherwise indicated, the term "aryl" refers to a polyunsaturated aromatic hydrocarbon substituent, which may be monocyclic or polycyclic (preferably 1 to 3 rings), which may be fused or covalently linked. The term "heteroaryl" refers to an aryl group (or aromatic ring) containing 1 to 4 heteroatoms selected from N, O and S, wherein the nitrogen and sulfur atoms may be optionally oxidized and the nitrogen atoms may be optionally quaternized. The heteroaryl group may be attached to the remainder of the molecule through a heteroatom. Non-limiting examples of aryl and heteroaryl groups include: phenyl, 1-naphthyl, 2-naphthyl, 4-biphenyl, 1-pyrrolyl, 2-pyrrolyl, 3-pyrazolyl, 2-imidazolyl, 4-imidazolyl, pyrazinyl, 2-oxazolyl, 4-oxazolyl, 2-phenyl-4-oxazolyl, 5-oxazolyl, 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl, 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, 2-furyl, 3-furyl, 2-thienyl, 3-thienyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidyl, 4-pyrimidyl, 5-benzothiazolyl, purinyl, 2-benzimidazolyl, 5-indolyl, 1-isoquinolinyl, 5-isoquinolinyl, 2-quinoxalinyl, 5-quinoxalinyl, 3-quinolyl and 6-quinolyl. The substituents for each of the above aryl and heteroaryl ring systems are selected from the following acceptable substituent groups.

For the sake of brevity, the term "aryl" when used in combination with other terms (e.g., aryloxy, arylthioxy, arylalkyl) includes both aromatic and heteroaromatic rings described above. Thus, the term "arylalkyl" is intended to include those radicals (e.g., benzyl, phenethyl, picolyl, and the like) in which an aryl group is attached to an alkyl group, including those alkyl groups in which a carbon atom (e.g., methylene) is substituted with, for example, an oxygen atom (e.g., phenoxymethyl, 2-pyridyloxymethyl, 3- (1-naphthyloxy) propyl, and the like).

Each of the above terms (e.g., "alkyl," "heteroalkyl," "aryl," and "heteroaryl") includes both substituted and unsubstituted forms of the specified radical. Preferred substituents for each type of radical are provided below.

Substituents for alkyl and heteroalkyl radicals (including those groups commonly referred to as alkenes, alkenyl, heteroalkenyl, alkynyl, cycloalkyl, heterocycloalkyl, cycloalkenyl, and heterocycloalkenyl) are commonly referred to as "alkyl substituents" and "heteroalkyl substituents," respectively, and they may be one or more selected from, but are not limited to, the following: -OR', = ONR ', = N-OR', -NR 'R ", -SR', -halogen, -SiR 'R" R' ", -OC (O) R ', -C (O) R', -CO₂R’,-CONR’R”,-OC(O)NR’R”,-NR”C(O)R’,-NR’-C(O)NR”R”’,-NR”C(O)₂R’,-NR-C(NR’R”R”’)=NR””,-NR-C(NR’R”)=NR”’,-S(O)R’,-S(O)₂R’,-S(O)₂NR’R”,-NRSO₂R', -CN and-NO₂And in an amount of 0 to (2m '+ 1), wherein m' is the total number of carbon atoms of the radical. R ', R ", R'" and R "" preferably each independently refer to hydrogen, a substituted or unsubstituted heteroalkyl, a substituted or unsubstituted aryl (e.g., aryl substituted with 1 to 3 halogens), a substituted or unsubstituted alkyl, alkoxy, or thioalkoxy, or an arylalkyl group. For example, when a compound of the invention includes more than one R group, each of the R groups is independently selected from the respective R ', R ", R'" and R "" groups (when more than one of these groups is present). When R' and R "are attached to the same nitrogen atom, they may combine with the nitrogen atom to form a 5-, 6-or 7-membered ring. For example, -NR' R "is intended to include, but is not limited to: 1-pyrrolidinyl and 4-morpholinyl. From the above discussion of substituents, those skilled in the art will appreciate that the term "alkyl" is intended to include the following groups: the group including carbon atoms attached to groups other than hydrogen groups, e.g. haloalkyl (e.g., -CF)₃and-CH₂CF₃) And acyl (e.g., -C (O) CH)₃，-C(O)CF₃，-C(O)CH₂OCH₃Etc.).

Similar to the description of substituents for alkyl radicals, aryl substituents and heteroaryl substituents are generally referred to as "aryl substituents" and "heteroaryl substituents", respectively, and are different and are selected from, for example, halogen, -OR ', = O, = NR ', = N-OR ', -NR ' R ", -SR ', -halogen, -SiR ' R" R ' ", -oc (O) R ', -c (O) R ', -CO₂R’，-CONR’R”，-OC(O)NR’R”，-NR”C(O)R’，-NR’-C(O)NR”R”’，-NR”C(O)₂R’，-NR-C(NR’R”)=NR”’，-S(O)R’，-S(O)₂R’，-S(O)₂NR’R”，-NRSO₂R', -CN and-NO₂，-R’，-N₃，-CH(Ph)₂Fluoro (C)₁-C₄) Alkoxy and fluoro (C)₁-C₄) Alkyl in an amount of 0 to the total number of open valences on the aromatic ring system, and wherein, R ', R ", R'" and R "" are preferably independently selected from: hydrogen, (C)₁-C₈) Alkyl and heteroalkyl, unsubstituted aryl and heteroaryl, (unsubstituted aryl) - (C)₁-C₄) Alkyl and (unsubstituted aryl) oxy- (C)₁-C₄) An alkyl group. For example, when a compound of the invention comprises more than one R group, each of the R groups is independently selected from the respective R ', R ", R'" and R "" groups (when more than one of these groups is present).

Two of the aryl substituents on adjacent atoms of the aromatic or heteroaromatic ring may optionally be substituted by a substituent of the formula-T-C (O) - (CRR')_q-U-, wherein T and U are independently-NR-, -O-, -CRR' -or a single chemical bond, and q is an integer from 0 to 3. Alternatively, two of the substituents on adjacent atoms of the aromatic or heteroaromatic ring may optionally be substituted by a group of formula-A- (CH)₂)_r-B-wherein A and B are independently-CRR' -, -O-, -NR-, -S (O)₂-,-S(O)₂NR' -or a single chemical bond, and r is an integer of 1 to 4. One of the individual chemical bonds in the new ring thus formed may be optionally substituted by a double bond. Alternatively, two of the substituents on adjacent atoms of the aryl or heteroaryl ring may be optionally substituted by a group of formula- (CRR')_s-X-(CR”R”’)_d-wherein S and d are independently an integer of 0 to 3, and X is-O-, -NR' -, -S (O)₂-, or-S (O)₂NR' -. The substituents R, R 'and R' are preferably independently selected from hydrogen or substituted or unsubstituted (C)₁-C₆) An alkyl group.

The term "heteroatom" as used herein includes oxygen (O), nitrogen (N), sulfur (S), phosphorus (P), and silicon (Si).

The term "aryloxy" as used herein refers to both-O-aryl and-O-heteroaryl, wherein aryl and heteroaryl are defined herein.

The term "pharmaceutically acceptable salt" as used herein refers to a salt that retains the biological efficacy and properties of the compounds of the present invention, and is neither biologically nor otherwise undesirable. In many cases, the compounds of the present invention are capable of forming acid and/or base salts via the presence of amino and/or carboxyl groups or similar groups, such as phenol or hydroxamic acid (hydroxyamic acid). Pharmaceutically acceptable acid addition salts may be formed by inorganic and organic acids. Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like. Organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like. Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases. Inorganic bases from which salts can be derived include, for example, sodium salts, potassium salts, lithium salts, ammonium salts, calcium salts, magnesium salts, iron salts, zinc salts, copper salts, manganese salts, aluminum salts, and the like, with ammonium salts, potassium salts, sodium salts, calcium salts, and magnesium salts being particularly preferred. Organic bases from which salts can be derived include, for example, primary, secondary and tertiary amines, substituted amines (including naturally occurring substituted amines), cyclic amines, basic ion exchange resins, and the like, such as, inter alia, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine. The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound, the basic group or the acidic group by conventional chemical methods. In general, such salts can be prepared by reacting the free acid forms of these compounds with a stoichiometric amount of a suitable base (e.g., sodium hydroxide, calcium hydroxide, magnesium hydroxide or potassium hydroxide, carbonates, bicarbonates, and the like) or by reacting the free base forms of these compounds with a stoichiometric amount of a suitable acid. These reactions are usually carried out in water or an organic solvent, or in a mixture of water and an organic solvent. Generally, in practice, non-aqueous media (e.g., ether, ethyl acetate, ethanol, isopropanol, or acetonitrile) are preferred. A list of other suitable salts can be found, for example, in Remington's Pharmaceutical Sciences, 20 th edition, Mack Publishing Company, Easton, Pa., (1985), which reference is incorporated herein by reference.

The term "pharmaceutically acceptable carrier/excipient" as used herein includes any and all solvents, dispersion media, coatings, surfactants, antioxidants, preservatives (e.g., antibacterial, antifungal agents), isotonic agents, absorption delaying agents, salts, drugs, drug stabilizers, binding agents, excipients, disintegrants, lubricants, sweeteners, flavorants, dyes, and the like, and combinations thereof known to those of ordinary skill in the art (see, e.g., Remington's Pharmaceutical Sciences, 18 th edition, Mack Printing Company,1990, pp.1289-1329, which reference is incorporated herein by reference). The above pharmaceutically acceptable carriers/excipients may be used in the therapeutic or pharmaceutical compositions in addition to any conventional carriers heretofore known to be incompatible with active ingredients.

The term "subject" as used herein refers to an animal. Preferably, the animal is a mammal. Subjects also refer to, for example, primates (e.g., humans), cows, sheep, goats, horses, dogs, cats, rabbits, rats, mice, fish, birds, and the like. In a preferred embodiment, the subject is a human.

Compounds and compositions

TM-Ln-AM (Ia)，

wherein TM is a targeting moiety, AM is an activating moiety that activates dendritic cells, natural killer cells, or tumor cells, or a combination thereof, Ln is a linker, and n is an integer selected from 0 and 1.

An "activating moiety" as used herein refers to a molecule or agent that is capable of stimulating or enhancing the immune system or tumor cells of an individual. In general, the activating moiety acts directly or indirectly on a toll-like receptor, a nucleotide-oligomerization domain-like receptor, a RIG-I-like receptor, a c-type lectin receptor, or a cytosolic DNA sensor, or a combination thereof.

In some embodiments, the activating moiety activates human immune cells including, but not limited to: dendritic cells, macrophages, monocytes, myeloid suppressor cells, NK cells, B cells, T cells.

Dendritic cells are the most potent antigen presenting cells. Dendritic cells play a major role in initiating innate and adaptive immune responses. Dendritic cells also play a key role in the induction and maintenance of immune tolerance.

By "Dendritic Cells (DCs)" herein is meant a heterogeneous population of cells, which includes two major subtypes, namely myeloid DC (mdc) and plasmacytoid DC (pdc) (Steinman et al, 1979, j.exp.med.,149, 1-16). These two subsets of blood DCs were initially distinguished by their expression of CD11c (integrin complement receptor) and CD123 (IL-3R α). Each of the pDC and mDC populations constitutes from about 0.2% to about 0.6% of the PBMC population in humans.

"pDC" herein refers to plasmacytoid dendritic cells, and they represent a subset of dendritic cells found in blood and peripheral lymphoid organs. These cells express the surface markers CD123, BDCA-2(CD303) and BDCA-4(CD304) and HLA-DR, but do not express CD11c, CD14, CD3, CD20 or CD56, which distinguishes pdcs from general dendritic cells, monocytes, T cells, B cells and NK cells. As a component of the innate immune system, these cells express intracellular Toll-like receptors 7 and 9, which enable the detection of viral and bacterial nucleic acids, such as ssRNA or CpG DNA motifs. Upon stimulation and subsequent activation, these cells produce large amounts of type I interferons (primarily IFN- α and IFN- β) and type III interferons (e.g., IFN- λ), which are important pleiotropic antiviral compounds that mediate a variety of effects. Plasmacytoid dendritic cells are widely involved in the innate immune response and the adaptive immune response of humans by producing large amounts of type I interferons, cytokines and chemokines. They modulate NK cells, T cells, B cells and other cells involved in the intensity, duration and pattern of the immune response, and therefore they play a very important role in tumors, infections and autoimmune diseases (Liu YJ. IPC: pathogenic type1 interference-producing cells and plasma dendritic cell procuring. Annu Rev Immunol.2005;23:275-306.Gilliet M, Cao W, Liu YJ. plasma dendritic cell: sensing nucleic acids in viral infection and autoimmune diseases. Nat Rev Immunol.2008Aug;8(8):594 606).

"mdcs" herein refer to myeloid dendritic cells, and they represent a subset of circulating dendritic cells found in blood and peripheral lymphoid organs. These cells express the surface markers CD11c, CD1a, HLA-DR and any of BDCA-1(CD1c) and BDCA-3(CD 141). They do not express BDCA-2 or CD123, which distinguishes mdcs from pdcs. mdcs also do not express CD3, CD20, or CD 56. As a component of the innate immune system, mdcs express Toll-like receptors (TLRs), including TLR2, TLR3, TLR4, TLR5, TLR6, and TLR8, which enable detection of bacterial and viral components. After stimulation and subsequent activation, these cells are the most potent antigen presenting cells, activating antigen-specific CD4 and CD8T cells. In addition, mdcs have the ability to produce large amounts of IL-12 and IL23, which is important for inducing Th 1-mediated or Th17 cell-mediated immunity.

It was found that many solid tumors (e.g., breast and head and neck, ovarian cancers) had pDC infiltration (Trelleux I, Blay JY, Bendriss-Vermark N et al, Dendritic cell infiltration and prognosis of early stage Breast Cancer, clin Cancer Res2004;10: 7466. Cancer E, Wollenberg B, Rothenfuel S et al, Identification and functional analysis of tumor-infiltrating Cancer and Cancer Res2003;63:6478-6487.Zou Med. Machelon V, Coulon-L' Hermin A, et al, molecular-derivative-1. tumor, tumor-invasion of tumor suppressor J. 2. tumor, tumor-metastasis, tumor-invasion of tumor, tumor suppressor J. 2. tumor, tumor-metastasis, tumor-1. tumor, tumor-metastasis, tumor-invasion of tumor, tumor invasion of tumor, tumor invasion of tumor, tumor invasion of tumor, tumor invasion of tumor, tumor invasion of tumor, tumor invasion of, chomuraat P, Broyles D et al, In Breast cancer tissue, anatomical polymeric cells with their derivatives In the term, where the areas of the areas are located In the term, J Exp Med1999, 190:1417-1425, Meniere-Caux C, Montmain G, Dieu MC et al, Inhibition of the differentiation of the polymeric cells from CD34(+) molecular by molecular cells: uke of interfacial-6 and macroporous polymeric-mechanical cells, 1998;92: 8: BLO 4791). These immature DC cells fail to play a role in enhancing anti-tumor immunity. In contrast, DCs in the tumor microenvironment promote tumor growth by inhibiting anti-tumor immunity and promoting angiogenesis. There is evidence that Toll-like receptor 7 agonist Imiquimod and Toll-like receptor9 agonist CpG drugs can stimulate pDC in the tumor microenvironment, thereby inhibiting tumor development (Dummer R, Urosevic M, Kempf W et al, Imiquode in basal cell Carcinoma: how tools it work.

Natural Killer (NK) cells are a class of cytotoxic lymphocytes that constitute a major component of the immune system. NK cells are a subset of peripheral blood lymphocytes defined by expression of CD56 or CD16 and lack of T cell receptor (CD 3). The natural killer cells recognize and kill transformed cell lines without eliciting MHC non-limiting means. NK cells play an important role in tumor suppression and protection of cells from viral infection. The process by which NK cells recognize target cells and deliver sufficient signal to trigger target lysis is determined by the large number of inhibitory and activating receptors on the cell surface. Differentiating NK itself from altered NK itself involves the recognition of MHC-1 molecules and non-MHC ligands such as CD48 and Clr-1b by inhibitory receptors. NK recognition of infected or injured cells (altered self) is regulated by stress-induced ligands (e.g., MICA, MICB, Rael, H60, Mult 1) or virus-encoded ligands (e.g., m157, hemagglutinin) recognized by various activating receptors, including NKG2D, Ly49H and NKp46/Ncr 1.

NK cells represent the predominant lymphoid cells in peripheral Blood months after allogeneic or autologous stem cell transplantation, and they play a major role in pathogen immunity during this period (Reititie et al (1989) Blood73: 1351-6868; Lowdell et al (1998) Bone Marrow Transplant21: 679-686). The role of NK cells in transplantation, graft-versus-host disease, anti-leukemic activity and post-transplantation infection is reviewed in: lowdell (2003) transfer Medicine13: 399-.

Human NK cells mediate lysis of tumor cells and lysis of virus-infected cells through natural cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC).

Human NK cells are controlled by positive and negative cytolytic signals. Negative (inhibitory) signals are transduced by C-plant lectin domains comprising the receptor CD94/NKG2A and some killer immunoglobulin-like receptors (KIR). The regulation of NK lysis by inhibitory signals is called the "self-deletion" hypothesis, in which specific HLA (class I allele expressed on the surface of target cells) binds to inhibitory receptors on NK cells. Down-regulation of HLA molecules on tumor cells and some virally infected cells (e.g., CMV) reduces this inhibition below a target threshold and target cells can become susceptible to NK cell-mediated lysis if they also carry NK triggering and activating molecules. TLR7, TLR8, or TLR9 agonists can activate both mdcs and pdcs, producing type I IFN and expressing co-stimulatory molecules such as GITR ligands, followed by activation of NK cells, producing IFN-g and effectively promoting NK cell killing function.

Inhibitory receptors are grouped into two groups, one is the Ig-superfamily known as killer immunoglobulin-like receptors (KIRs) and the other is the plant lectin family (NKG 2, which forms a dimer with CD94 on the cell surface). KIR has an extracellular structure of 2 domains or an extracellular structure of 3 domains and binds to HLA-A, HLA-B or HLA-C. The NKG2/CD94 complex binds HLA-E.

Inhibitory KIRs have up to 4 intracellular domains that contain ITIM and the best characterized inhibitory KIRs are KIR2DL1, KIR2DL2, and KIR2DL3, which are known to bind to HLA-C molecules. KIR2DL2 and KIR2DL3 bind to the first set of HLA-C alleles, while KIR2DL1 binds to the second set of alleles. Some leukemia/lymphoma cells express both a first set of HLA-C alleles and a second set of HLA-C alleles and are known to be resistant to NK-mediated cytolysis.

With respect to positive activation signals, ADCC is thought to be mediated by CD16, and a number of trigger receptors have been identified that cause natural cytotoxicity, including CD2, CD38, CD69, NKRP-I, CD40, B7-2, NK-TR, NKp46, NKp30, and NKp 44. In addition, several KIR molecules with short intracytoplasmic tails are also stimulatory. These KIRs (KIR 2DS1, KIR2DS2 and KIR2DS 4) are known to bind HLA-C, and their extracellular domains are identical to their associated inhibitory KIRs. Activation of KIRs lacks ITIM, but instead binds to DAP12, which leads to NK cell activation. The mechanisms controlling the expression of inhibitory and activating KIRs remain unclear.

Several reports have described the expression of TLRs in mouse or human cancers or cancer cell lines. For example, TLRs 1-6 are expressed by colon, lung, prostate and melanoma mouse tumor cell lines (Huang B et al, Toll-like receptors on tumor cells efficacy evaluation of tumor tissue. cancer cell. cancer Res.2005;65(12): 5009) and TLR3 is expressed in Human breast cancer cells (Salaun B, Coste I, Risson MC, Lebecqe SJ, Renno T.3TLR cancer cell. J Immunol.2006;176(8): 4894) and liver cancer and stomach cancer cells express TLR2 and TLR4 (Huang B et al, Listeria mongenes tumor tissue cancer cell. J. Immunol.2007; fire cancer cell. 52. beer W439; fire cancer cell. 52. RTM. and 5. RTM. 5. and 5. RTM. C. 5. and TLR. 5. RTM. 5. C. and 5. RTM. and 5. RTM. 5. RTM. C. and 5. RTM. 5. and 5. RTM. 5. RTM. and similar to 5. RTM. 5. RTM. 5. and similar to 5. RTM. 2. RTM. 5. best. and similar to 5. RTM. best. RTM. 2. best. transferred to 5. transferred to Human tumor cells of cancer cell. transferred by cancer cell strain No. 5. transferred to 5 2859) is expressed by human lung cancer cells. TLR7 and TLR8 (Cherfils-Vicini J, Platonova S, Gillard M, Laurans L, Validire P, Caliandro R, Magdeleeinat P, Mami-Chouaib F, Dieu-Nosjean MC, Fridman WH, Damotte D, Saut des-Fridman C, Cremer I.J.Clin invest.2010;120(4): 1285) -1297) are found in human lung cancer tumor cells.

TLRs are a family of proteins that sense microbial products and/or initiate an adaptive immune response. TLRs activate Dendritic Cells (DCs). TLRs are conserved transmembrane molecules containing a leucine-rich repeat unit extracellular domain, transmembrane domain, and intracellular TIR (Toll/interleukin receptor) domain. TLRs recognize different structures in microorganisms, commonly referred to as "PAMPs" (pathogen-associated molecular patterns). Ligands that bind to TLRs initiate a cascade of intracellular signaling pathways that induce the production of factors involved in inflammation and immunity.

In some embodiments, the activating moiety is a TLR7 and/or TLR8 agonist. TLR7 and TLR8 are phylogenetically and structurally related. TLR7 is selectively expressed by human pDC and B cells. TLR8 is expressed primarily by mdcs, monocytes, macrophages and myelosuppressive cells. TLR 7-specific agonists activate plasma cell-like dc (pdc), thereby producing large amounts of type1 IFN and expressing high levels of co-stimulatory molecules that promote activation of T cells, NK cells, B cells, and mdcs. TLR 8-specific agonists activate myeloid DCs, monocytes, macrophages or myeloid suppressor cells, thereby producing large amounts of type1 IFN, IL-12 and IL-23 and expressing high levels of MHC class I, class II and co-stimulatory molecules that promote activation of antigen-specific CD4 and CD8+ T cells.

TM-L-AM (Ib)

wherein TM is a targeting moiety, L is a linker, AM is an activating moiety represented by the structure of formula (I):

x is S or-NR₁，R₁is-W₀—W₁—W₂—W₃—W₄；

W₀Is a bond, alkyl, alkenyl, alkynyl, alkoxy or-alkyl-S-alkyl- -,

W₂is a chemical bond, -O, -C (O), -C (S) or-S (S)O)₂—，

W₄is hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, aryl, aryloxy, heteroaryl or heterocyclyl, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, - - -NH₂Nitro, -alkyl-hydroxy, -alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，--C(O)-O-R₄，--S-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，--NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN, - -NO₂and-SH, wherein R₄Independently hydrogen, alkyl, alkenyl, - -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl;

r is hydrogen, alkyl, alkoxy, halogenated alkyl, halogen, aryl or heteroaromatic(ii) each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, - - -NH₂Nitro, -alkyl-hydroxy, -alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄，--C(O)-NH-R₄，--C(O)-NR₄R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，--C(O)-O-R₄，--O-C(O)-R₄，--S-R₄，--C(O)-S-R₄，--S-C(O)-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，--NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN and- -SH, in which R₄Independently hydrogen, alkyl, alkenyl, alkoxy, -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl, or haloalkyl;

n is 0,1, 2,3 or 4;

wherein R is₆，R₇And R₈Independently hydrogen, alkyl, alkenyl, alkoxy, alkylamino, dialkylamino, alkylthio, arylthio, - -alkyl-hydroxy, - -alkyl-C (O) -O-R₉- - (O) -R- -alkyl-C₉or-alkyl-O-C (O) -R₉Wherein R is₅Each independently is hydrogen, alkyl halideA radical of formula (I), an alkyl-aryl or an alkyl-heteroaryl, wherein R₉Is hydrogen, alkyl, alkenyl, halogen or haloalkyl;

optionally, X and Z together may form a 5 to 9 membered ring.

In some embodiments, X in formula (I) is S.

In some embodiments, X in formula (I) is-NR₁,R₁Is alkyl, -alkyl-W₄- - (O- -W) alkyl-O- -W₄- - (O) - -W- -NH- -C₄- - (O) - -W- -NH- -C (O)₄- - (O) - -NH- -W- -NH- -C₄- - (O) - -NH- -W- -NH- -C₄- - -alkyl-S (O)₂-W₄or-alkyl-NH-C (S) -W₄Wherein W is₄As defined above.

In some embodiments, Z in formula (I) is hydrogen, alkyl, alkoxy, aryl, heteroaryl, haloalkyl, each of which is optionally substituted with one to three substituents selected from the group consisting of: hydroxy, alkyl, aryl, heteroaryl, heterocyclyl, cyano, - - (O- -alkoxy) -alkyl, nitro and- -N (R)₅)₂Wherein R is₅Each independently hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkyl-heteroaryl.

In some embodiments, Y in formula (I) is-NH₂- - (O- -NH) - -alkyl-NH₂Each of which is optionally substituted with one to three substituents selected from alkyl, alkoxy, alkenyl, and alkynyl.

In some embodiments, n in formula (I) is 1 or 2.

In some embodiments, R in formula (I) is aryl or heteroaryl, each of which is optionally substituted with one to three substituents selected from the group consisting of: hydroxy, alkoxy, -alkyl-hydroxy, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄,--C(O)-R₄,--C(O)-NH-R₄,--C(O)-NR₄R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄,--C(O)-O-R₄,--O-C(O)-R₄,--S-R₄,--C(O)-S-R₄,--S-C(O)-R₄,--S(O)₂-R₄,--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄,--NHR₄,--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN and- -SH, in which R₄Independently hydrogen, alkyl, alkenyl, alkoxy, -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl.

TM-L-AM (Ib)

wherein,is the point to be connected to the connector;

R₁₀and R₁₁Independently hydrogen, alkyl, alkenyl, aryl, haloalkyl, heteroaryl, heterocyclyl or cycloalkyl, each of which is substituted with one or oneOptionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, halogen, - -N (R)₅)₂-alkoxy-alkyl, -alkoxy-alkenyl, -C (O) -alkyl, -C (O) -O-alkyl, -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl, and- -CO-heteroaryl, wherein R is₅Each independently is hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkyl-heteroaryl; TM and L are defined as described above and below.

Targeting moieties

In general, the compounds of the present invention comprise a targeting moiety.

By "Targeting Moiety (TM)" or "targeting agent" herein is meant a molecule, complex or aggregate that specifically or selectively binds to a molecule, cell, particle, tissue or aggregate of interest, which is commonly referred to as a "target" or "marker" and which is discussed in further detail herein.

In some embodiments, the targeting moiety comprises an immunoglobulin, a protein, a peptide, a small molecule, a nanoparticle, or a nucleic acid.

Exemplary targeting agents such as antibodies (e.g., chimeric, humanized and human), ligands for receptors, plant lectins and sugars, and substrates for some enzymes, are recognized in the art and are not limiting for use in the practice of the invention. Other targeting agents include a class of compounds: the compounds do not include specific molecular recognition motifs, and include nanoparticles that add molecular weight to the activated moiety, macromolecules such as poly (ethylene glycol), polysaccharides, and polyamino acids. The additional molecular weight affects the pharmacokinetics of the activated moiety, such as serum half-life.

In some embodiments, the targeting moiety is an antibody, antibody fragment, bispecific antibody or other antibody-based molecule or compound. However, other examples of targeting moieties are known in the art and other examples of targeting moieties may be used, e.g., aptamers, avimers, receptor binding ligands, nucleic acids, biotin-avidin binding pairs, binding peptides or proteins, and the like. The terms "targeting moiety" and "binding moiety" are used synonymously herein.

By "target" or "label" herein is meant any entity capable of specifically binding to a particular targeting moiety. In some embodiments, the target is specifically associated with one or more specific cell or tissue types. In some embodiments, the target is specifically associated with one or more specific disease states. In some embodiments, the target is specifically associated with one or more specific developmental stages. For example, the expression level of a cell type-specific marker in that cell type is typically at least two-fold higher than the expression level in a reference cell population. In some embodiments, the level of the cell-type specific marker is at least 3-fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, at least 10-fold, at least 50-fold, at least 100-fold, or at least 1,000-fold greater than the average expression in the reference population. Detection or measurement of cell type specific markers can distinguish one target cell type or multiple target cell types from many, most, or all other cell types. In some embodiments, the target may comprise a protein, carbohydrate, lipid, and/or nucleic acid as described herein.

A substance may be considered "targeted" for the purposes described herein if it specifically binds to a targeting moiety of a nucleic acid. In some embodiments, the nucleic acid targeting moiety specifically binds to the target under stringent conditions. A complex or compound of the invention containing a targeting moiety is considered to be "targeted" if the targeting moiety specifically binds to the target, thereby delivering the entire complex or compound composition to a particular organ, tissue, cell, extracellular matrix component, and/or intracellular compartment.

In some embodiments, a compound according to the invention comprises a targeting moiety that specifically binds to one or more targets (e.g., antigens) associated with an organ, tissue, cell, extracellular matrix component, and/or intracellular compartment. In some embodiments, the compound comprises a targeting moiety that specifically binds to a target associated with a particular organ or organ system. In some embodiments, a compound according to the invention comprises a nuclear targeting moiety that specifically binds to one or more intracellular targets (e.g., organelles, intracellular proteins). In some embodiments, the compound comprises a targeting moiety that specifically binds to a target associated with a diseased organ, tissue, cell, extracellular matrix component, and/or intracellular compartment. In some embodiments, the compound comprises a targeting moiety that specifically binds to a target associated with a particular cell type (e.g., endothelial cells, cancer cells, malignant cancer cells, prostate cancer cells, etc.).

In some embodiments, compounds according to the invention comprise a targeting moiety that binds to a target specific for one or more specific tissue types (e.g., liver tissue and prostate tissue). In some embodiments, compounds according to the invention comprise a targeting moiety that binds to a target specific for one or more specific cell types (e.g., T cells and B cells). In some embodiments, compounds according to the invention comprise a targeting moiety that binds to a target specific for one or more specific disease states (e.g., tumor cells and healthy cells). In some embodiments, compounds according to the invention comprise a targeting moiety that binds to a target specific for one or more particular developmental stages (e.g., stem cells and differentiated cells).

In some embodiments, the target may be a marker that is associated only or predominantly with one or several cell types, one or several diseases and/or one or several developmental stages. The level of expression of a cell-type specific marker in that cell type is typically at least two-fold higher than the level of expression in a reference cell population, which may, for example, be comprised of a mixture containing nearly equal amounts of cells from a plurality (e.g., 5-10, or more) different tissues or organs. In some embodiments, the expression level of the cell-type specific marker is at least 3-fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, at least 10-fold, at least 50-fold, at least 100-fold, or at least 1000-fold higher than the average expression level in the reference population. Detection and measurement of cell type specific markers can distinguish one target cell type or multiple target cell types from many, most, or all other cell types.

In some embodiments, the target comprises a protein, a carbohydrate, a lipid, and/or a nucleic acid. In some embodiments, the target comprises a protein and/or characteristic portions thereof, e.g., a tumor marker, an integrin, a cell surface receptor, a transmembrane protein, an intercellular protein, an ion channel, a membrane transporter, an enzyme, an antibody, a chimeric protein, a glycoprotein, and the like. In some embodiments, the target comprises a carbohydrate and/or characteristic portions thereof, e.g., a glycoprotein, a sugar (e.g., a monosaccharide, a disaccharide, a polysaccharide), a polysaccharide coating (i.e., a peripheral region enriched in carbohydrates on the outer surface of most eukaryotic cells), and the like. In some embodiments, the target comprises a lipid and/or characteristic portion thereof, e.g., an oil, a fatty acid, a glyceride, a hormone, a steroid (e.g., cholesterol, bile acid), a vitamin (e.g., vitamin E), a phospholipid, a sphingolipid, a lipoprotein, and the like. In some embodiments, the target comprises a nucleic acid and/or characteristic portions thereof, e.g., a DNA nucleic acid, an RNA nucleic acid, a modified DNA nucleic acid, a modified RNA nucleic acid, a nucleic acid comprising any combination of DNA, RNA, modified DNA, and modified RNA.

A variety of markers are known in the art. Typical markers include cell surface proteins, e.g., receptors. Exemplary receptors include, but are not limited to: transferrin receptor, LDL receptor, growth factor receptor (e.g., epidermal growth factor receptor family members (e.g., EGFR, Her2, Her3, Her 4)) or vascular endothelial growth factor receptor, cytokine receptor, cell adhesion molecule, integrin, selectin and CD molecule. The marker may be a molecule present only or in large amounts on malignant cells, e.g., a tumor antigen.

In some embodiments, the targeting moiety specifically binds to a tumor cell, or preferentially binds to a tumor cell relative to a non-tumor cell.

Binding of the targeting moiety to the tumor cell can be detected using methods known in the art.

In some embodiments, the tumor cell is a cancer cell, a sarcoma cell, a lymphoma cell, a myeloma cell, or a central nervous system cancer cell.

In some embodiments, the targeting moiety is capable of specifically binding to a tumor antigen, or preferentially binds to a non-tumor antigen relative to a non-tumor antigen.

By "specific binding" or "preferential binding" herein is meant that binding between two binding partners (e.g., between a targeting moiety and its binding partner) is selective for both binding partners and can be distinguished from unwanted or non-specific interactions. For example, the ability of an antigen-binding moiety to bind to a specific epitope can be measured by enzyme-linked immunosorbent assay (ELISA) or other techniques familiar to those skilled in the art (e.g., surface plasmon resonance (analysis on BIAcore instruments) (Liljeblad et al, Glyco J17,323-329 (2000)) and conventional binding assays (Heeley, Endocr Res28,217-229 (2002)). The term "anti- [ antigen]Antibodies "and" with [ antigens ]]A bound antibody "refers to an antibody that is capable of binding the respective antigen with sufficient avidity such that the antibody binds to the respective antigenFor use as diagnostic and/or therapeutic agents for targeting antigens. In some embodiments, the anti- [ antigen]The extent to which an antibody binds to an unrelated protein is less than about 10% of the extent of antibody-antigen binding measured (e.g., by Radioimmunoassay (RIA)). In some embodiments, binding [ antigen]Has a dissociation constant (KD) of less than 1 [ mu ] M, less than 100nM, less than 10nM, less than 1nM, less than 0.1nM, less than 0.01nM, or less than 0.001nM (e.g., 10 nM)^-8M or less, e.g. 10^-8M to 10^-13M, e.g. 10^-9M to 10^-13M). It will be appreciated that the above definitions also apply to antigen binding moieties that bind to an antigen.

In some particular embodiments, the target is a tumor marker. In some embodiments, a tumor marker is an antigen present in a tumor that is not present in normal organs, tissues, and/or cells. In some embodiments, the tumor marker is an antigen that is more prevalent in tumors than in normal organs, tissues, and/or cells. In some embodiments, the tumor marker is an antigen that is more prevalent in malignant cancer cells than in normal cells.

By "tumor antigen" herein is meant an antigenic substance produced in a tumor cell, i.e., a substance that triggers an immune response in a host. Normal proteins in vivo are not antigenic due to self-tolerance, which is a process that: in this process, autoreactive Cytotoxic T Lymphocytes (CTL) and autoantibody producing B lymphocytes are "centrally" knocked out in primary lymphoid tissue (BM) and "peripherally" knocked out in secondary lymphoid tissue (the T cell producing majority thymus and B cell producing spleen/lymph nodes). Thus, any protein not exposed to the immune system triggers an immune response. This may include normal proteins that are completely sequestered from the immune system, proteins that are normally produced in minute amounts, proteins that are normally produced only at certain developmental stages, or proteins whose structure has been altered by mutation.

In some embodiments, the target is preferentially expressed in tumor tissue and/or cells relative to expression in normal tissue and/or cells.

In some embodiments of the invention, the marker is a tumor marker. The marker may be a polypeptide that is expressed at a higher level in dividing cells than in non-dividing cells. For example, Her-2/neu (also known as ErbB-2) is a member of the EGF receptor family and is expressed on the surface of tumor cells associated with breast cancer. Another example is the peptide named F3, which is a suitable targeting agent for directing nanoparticles to nucleolin (Porkka et al, 2002, Proc. Natl. Acad. Sci., USA,99:7444; and Christian et al, 2003, J.cell biol.,163: 871). Targeting particles comprising nanoparticles and a10 aptamer (specifically binding to PSMA) were shown to be capable of specifically and efficiently delivering docetaxel to prostate cancer tumors.

Antibodies or other drugs that specifically target these tumor targets specifically interfere with and modulate signaling pathways of tumor cell biological behavior, directly modulate or block signaling pathways, thereby inhibiting tumor cell growth or inducing apoptosis. To date, several dozens of targeted drugs have been approved for clinical research and treatment of solid tumors or hematological malignancies, and many have been used for hematological malignancies.

In some embodiments, the tumor antigen (or tumor target) is selected from: CD2, CD19, CD20, CD22, CD27, CD33, CD37, CD38, CD40, CD44, CD47, CD52, CD56, CD70, CD79 and CD 137.

In some embodiments, the tumor antigen (or tumor target) is selected from: 4-1BB,5T4, AGS-5, AGS-16, angiopoietin 2, B7.1, B7.2, B7DC, B7H1, B7H2, B7H3, BT-062, BTLA, CAIX, carcinoembryonic antigen, CTLA4, Cripto, ED-B, ErbB1, ErbB2, ErbB3, ErbB4, EGFL 4, EpCAM, EphA 4, EphB 4, FAP, fibronectin, folate receptor, ganglioside GM 4, GD 4, glucocorticoid-induced tumor necrosis factor receptor (GIpA TR), gp100, g3672, GPNMB, ICOS, IGF 14, integrin α v β, KIR, TAG-3, Lewis Y antigen, mesothelin, c-MET MN, MUMN, MUC-5, MUSC-4, MUSCH 72, TROCH-4, TROCH 4, TRACP 4, TRACK 4, TRACP 4, CROCH-4, CRACH-4, CROCH-4, CRAT-4, CROCH-4, CR, VEGFR-2, VEGFR-3 and variants thereof. Variants of tumor antigens include various mutants or polymorphs known in the art and/or naturally occurring.

In some embodiments, the targeting moiety comprises an antibody or a functional fragment thereof.

As used herein, "immunoglobulin" or "antibody" refers to a full-length (i.e., naturally occurring or formed by the process of recombination of normal immunoglobulin gene fragments) immunoglobulin molecule (e.g., an IgG antibody) or immunologically active (i.e., specifically binding) portion of an immunoglobulin molecule, e.g., an antibody fragment. Within the scope of the claimed invention, the antibody or antibody fragment may be conjugated or derivatized. These antibodies include IgGl, lgG2a, IgG3, IgG4 (and IgG4 subtype) and IgA isotype.

The term "antibody" is used herein in its broadest sense and encompasses a variety of different antibody structures, including but not limited to: monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), and antibody fragments so long as they exhibit the desired antigen binding activity and comprise an Fc region of an immunoglobulin or a region equivalent to the Fc region. The terms "full-length antibody," "intact antibody," and "whole antibody" are used interchangeably herein to refer to an antibody having a structure substantially similar to a native antibody structure or an antibody having a heavy chain with an Fc region as defined herein.

"Natural antibody" herein refers to naturally occurring immunoglobulin molecules having different structures. For example, a native IgG antibody is a heterotetrameric glycoprotein of about 150,000 daltons, composed of two identical light chains and two identical heavy chains linked by disulfide bonds. From N-terminus to C-terminus, each heavy chain has a variable region (VH), also known as the variable heavy chain domain or heavy chain variable domain, followed by three constant domains (CH 1, CH2, and CH 3), also known as heavy chain constant regions. Similarly, from N-terminus to C-terminus, each light chain has a variable region (VL), also known as a variable light chain domain or light chain variable domain, followed by a constant light chain domain (CL), also known as a light chain constant region. The light chain of an antibody can be assigned to one of two types (called kappa and lambda) based on the amino acid sequence of the antibody constant domain.

An "antibody fragment" herein refers to a molecule other than an intact antibody, which comprises a portion of an intact antibody that binds an antigen that is bound to the intact antibody. Examples of antibody fragments include, but are not limited to: fv, Fab, Fab ', Fab ' -SH, F (ab ')2, diabodies, linear antibodies, single-chain antibody molecules (e.g., scFv), single-domain antibodies, and multispecific antibodies formed from antibody fragments. For a review of some antibody fragments see Hudson et al, Nat Med9,129-134 (2003). For reviews on scFv fragments see, for example, Pliickthun, in The Pharmacology of Monoclonal Antibodies, vol.113, Rosenburg and Moore editors, Springer-Verlag, New York, pp.269-315(1994), and WO93/16185, and U.S. Pat. Nos. 5,571,894 and 5,587,458. For a discussion of Fab and F (ab')2 fragments containing salvage receptor binding epitope residues and having improved in vivo half-life, see U.S. patent No. 5,869,046. Diabodies are antibody fragments with two antigen binding sites that can be bivalent or bispecific. See, for example, EP404,097, WO1993/01161, Hudson et al, Nat Med9,129-134(2003), and Hollinger et al, Proc Natl Acad Sci USA90, 6444-. Tri-and tetrad antibodies are also described in Hudson et al, Nat Med9,129-134 (2003). A single domain antibody is an antibody fragment that comprises all or a portion of a heavy chain variable domain of an antibody or all or a portion of a light chain variable domain of an antibody. In some embodiments, the single domain antibody is a human single domain antibody (Domantis, Inc., Waltham, MA; see, e.g., U.S. Pat. No.6,248,516 Bl). Antibody fragments can be prepared by a variety of different techniques including, but not limited to: as described herein, proteolytic digestion of intact antibodies and production by recombinant host cells (e.g., e.coli or phage).

An "antigen binding domain" herein refers to a portion of an antibody that comprises a region that specifically binds to and is complementary to all or a portion of an antigen. The antigen binding domain may be provided, for example, by one or more antibody variable domains (also referred to as antibody variable regions). In particular, the antigen binding domain comprises an antibody light chain variable region (VL) and an antibody heavy chain variable region (VH).

The "variable region" or "variable domain" herein refers to an antibody heavy or light chain domain involved in binding an antibody to an antigen. The variable domains of the heavy and light chains of natural antibodies (VH and VL, respectively) typically have similar structures, with each domain comprising four conserved Framework Regions (FR) and three hypervariable regions (HVRs). See, e.g., Kindt et al, Kuby Immunology, sixth edition, w.h.freeman and co., page 91 (2007). A single VH or VL domain may be sufficient to confer antigen binding specificity.

By "hypervariable region" or "HVR" herein is meant the various regions of an antibody variable domain which are highly variable in sequence and/or form structurally defined loops ("hypervariable loops"). In general, natural four-chain antibodies comprise six HVRs, three in VH (H1, H2, H3) and three in VL (L1, L2, L3). HVRs typically contain amino acid residues from hypervariable loops and/or amino acid residues from Complementarity Determining Regions (CDRs) that have the highest sequence variability and/or are involved in antigen recognition. In addition to the CDR 1in VH, the CDR typically comprises amino acid residues that form a hypervariable loop. Hypervariable regions (HVRs) are also referred to as "Complementarity Determining Regions (CDRs)" and these terms are used interchangeably herein in relation to the variable region portions that form the antigen-binding regions. This particular region has been described by Kabat et al, U.S. Dept. of Health and Human Services, Sequences of Proteins of Immunological Interest (1983) and Chothia et al, J Mol Biol196:901-917(1987), wherein an overlap or subset comprising amino acid residues is defined when compared to each other. However, the use of any definition with respect to a CDR of an antibody or variant thereof is intended to be within the scope of the terms defined and used herein. The exact number of residues comprising a particular CDR will vary depending on the sequence and size of the CDR. Given the amino acid sequence of the variable region of an antibody, one skilled in the art can routinely determine which residues comprise a particular CDR.

The antibody of the invention may be a chimeric antibody, a humanized antibody, a human antibody or an antibody fusion protein.

By "chimeric antibody" herein is meant a recombinant protein comprising the variable domains of both the heavy and light chains of an antibody, including the Complementarity Determining Regions (CDRs) of an antibody derived from one species, preferably a rodent antibody, more preferably a murine antibody, while the constant domains of the antibody molecule are derived from the constant domains of a human antibody. For veterinary applications, the constant domains of the chimeric antibody may be derived from constant domains of other species, e.g., human-like primates, cats or dogs.

"humanized antibody" herein refers to recombinant proteins as follows: in the recombinant protein, CDRs of an antibody from one species (e.g., a rodent antibody) are transferred from the variable heavy and variable light chains of the rodent antibody into the human heavy and light chain variable domains. The constant domains of the antibody molecules are derived from the constant domains of human antibodies. In some embodiments, specific residues of the framework regions of the humanized antibody, particularly those contacting or near the CDR sequences, can be modified, e.g., replaced by corresponding residues from the original rodent, human primate, or other antibody.

"human antibody" herein refers to an antibody obtained, for example, from a transgenic mouse that has been "engineered" to produce a particular human antibody in response to antigenic stimulation. In this technique, elements of the human heavy chain locus and the human light chain locus are introduced into mouse strains derived from embryonic stem cell lines that contain targeted disruptions of the endogenous heavy chain locus and light chain locus. Transgenic mice can synthesize human antibodies specific for human antigens, and mice can be used to generate human antibody-secreting hybridomas. Methods for obtaining human antibodies from transgenic mice are described by Green et al, Nature Genet.7:13(1994), Lonberg et al, Nature368:856(1994), Taylor et al, int.Immun.6:579 (1994). Fully human antibodies can also be constructed by gene transfection or chromosome transfection methods, as well as phage display techniques, all of which are known in the art. See, e.g., McCafferty et al, Nature348:552-553(1990) which describes the in vitro production of human antibodies and fragments thereof by immunoglobulin variable domain gene lineages from non-immunized donors. In this technique, antibody variable domain genes are cloned in-frame into the major or minor coat protein genes of filamentous phage and displayed as functional antibody fragments on the surface of the phage particle. Because the filamentous particle comprises a single-stranded DNA copy of the phage genome, selection based on the functional properties of the antibody also results in selection of a gene encoding an antibody exhibiting those properties. In this way, the phage mimics some of the properties of the B cell. Phage display can be performed in a variety of formats, for review of phage display see, e.g., Johnson and Chiswell, Current Opinion in Structural Biology3:5564-571 (1993). Human antibodies can also be produced by activating B cells in vitro. See U.S. patent nos. 5,567,610 and 5,229,275, the entire contents of which are incorporated herein by reference.

An "antibody fusion protein" herein refers to an antigen-binding molecule produced by recombination in which two or more of the same or different natural antibodies, single-chain antibodies or antibody fragments having the same or different specificities are linked. The fusion protein comprises at least one specific binding site. The valency of the fusion protein refers to the total number of binding arms or binding sites that the fusion protein has to bind to the antigen or epitope, i.e., monovalent, divalent, trivalent, or multivalent. By multivalent antibody fusion proteins is meant that the antibody fusion protein can utilize multiple interactions for binding to an antigen, thus increasing avidity for binding to the antigen or to a different antigen. Specificity refers to how many different types of antigens or epitopes an antibody fusion protein is capable of binding, i.e., monospecific, bispecific, trispecific, multispecific. Using these definitions, a natural antibody (e.g., IgG) is bivalent because it has two binding arms, but it is monospecific because it binds one type of antigen or epitope. Monospecific multivalent fusion proteins have more than one binding site for the same antigen or epitope. For example, a monospecific diabody is a fusion protein with two binding sites that react with the same antigen. The fusion protein may comprise multivalent or multispecific combinations of different antibody components or multiple copies of the same antibody component. The fusion protein may further comprise a therapeutic agent.

In some embodiments, the targeting moiety is an antibody or antibody fragment, which targeting moiety is selected based on its specificity for an antigen that is expressed on a target cell or target of interest. A variety of different tumor-specific or other disease-specific antigens have been identified, and antibodies to those antigens have been used or planned for use in the treatment of these tumors or other diseases. Antibodies known in the art may be used with the compounds of the invention, particularly for the treatment of diseases associated with the target antigen. Examples of target antigens (and their associated diseases) that can be targeted by the antibody-linker-drug conjugates of the invention include: CD2, CD19, CD20, CD22, CD27, CD33, CD37, CD38, CD40, CD44, CD47, CD52, CD56, CD70, CD79, CD137,4-1BB,5T4, AGS-5, AGS-16, angiopoietin 2, B7.1, B7.2, B7 4, B7H 4, B7H 4, B7H 4, BT-062, BTLA, CAIX, carcinoembryonic antigen, CTLA4, Cripto, ED-B, ErbB4, ErbB4, ErbB4, EGFL 4, EpvhA 4, EphA 4, EphB 4, FAP, fibronectin, folate receptor, ganglioside 4, GM 4, glucocorticoid receptor, tumor necrosis factor receptor induced by glucocorticoid receptor (PGCAGC-AG), PGA-PGA 4, PGHA-4, PGA-4, PGCA-4, PGX-4, PGA-4, PGS-B-binding protein, PGS-receptor, PGS-C-4, PGS-receptor, PGS-binding protein, PGS-receptor, PGA-3, PGS-X, PGS-3, PGS-C-4, PGS-X, TACI, TAG-72, tenascin, TIM3, TRAILR1, TRAILR2, VEGFR-1, VEGFR-2, VEGFR-3.

In some embodiments, the antibody is selected from the group consisting of: rituximab, herceptin, trastuzumab, erbitux, verterby, alemtb, arzerra, (ofatumumab), Benlysta (belimumab), Yervoy, Perjeta (pertuzumab), Tremelimumab, Nivolumab, Dacetuzumab, Urelumab, MPDL3280A, Lambrolizumab and blinatumumab.

Rituximab (rituximab) is a chimeric antibody used to treat B-cell non-hodgkin's lymphoma. It acts on the surface of B cells expressing the CD20 antigen, CD20 antigen is expressed on 90% of B cell non-hodgkin lymphomas. Rituximab binds to CD20, thereby inducing B-cells to lyse by CDC and ADCC and sensitize human lymphocytes resistant to some cytotoxic chemotherapy.

Herceptin (trastuzumab) is a humanized monoclonal antibody that acts on the extracellular domain of Her2 human epidermal growth factor receptor, Her2 is expressed in 25% to 30% of breast cancers. Trastuzumab is considered to have an antitumor effect by three aspects: (1) down-regulating Her2 receptor, inhibiting Her2 intracellular signaling pathway and inducing apoptosis; (2) immune mechanisms that link antibody-dependent ADCC and CDC to killing of tumor cells; (3) can improve the effect of chemotherapy.

Erbitux (cetuximab) is a chimeric antibody that acts on Epidermal Growth Factor Receptor (EGFR). Erbitux binds EGFR, thereby inhibiting the signal transduction pathway of EGFR, affecting cell proliferation, infiltration and metastasis, and angiogenesis. Inhibition of the EGFR signaling pathway can improve the efficacy of chemotherapeutic drugs and the efficacy of radiation therapy.

Avastin (bevacizumab) is a humanized monoclonal antibody targeting Vascular Endothelial Growth Factor (VEGF). Binding of avastin to VEGFR inhibits VEGF and signal transduction, thereby inhibiting tumor angiogenesis.

Other antibodies currently under development may also be used as targeting moieties. For example, therapeutic monoclonal antibodies directed against the following targets are being developed for the treatment of tumors: CD2, CD19, CD20, CD22, CD27, CD33, CD37, CD38, CD40, CD44, CD47, CD52, CD56, CD70, CD79, CD 137. And therapeutic monoclonal antibodies directed against the following targets are also being developed for the treatment of tumors: 4-1BB,5T4, AGS-5, AGS-16, angiopoietin 2, B7.1, B7.2, B7DC, B7H1, B7H2, B7H3, BT-062, BTLA, CAIX, carcinoembryonic antigen, CTLA4, Cripto, ED-B, ErbB1, ErbB2, ErbB3, ErbB4, EGFL 4, EpCAM, EphA 4, EphB 4, FAP, fibronectin, folate receptor, ganglioside GM 4, GD 4, glucocorticoid-induced tumor necrosis factor receptor (GIpA TR), gp100, g3672, GPNMB, ICOS, IGF 14, integrin α v, β v β, KIR, TAG-3, Lewis, mesothelin, c-MET, MUC-IX, MUC-5, MUC-72, MURC-4, VEGFR-72, TRACCH-4, GEN, TRACCH-4, TRAC 4, TRACCH-4, VEGFR-72, PGR-4, PGMC-4, PGR-11, PGMC-4, PGR-36, VEGFR-3, and variants thereof. (Scott AM, Wolchok JD, Old LJ. antibody Therapy of cancer. nat Rev cancer.2012Mar22;12(4): 278-87).

In some embodiments, the targeting moiety comprises a Fab, Fab ', F (ab')2, single domain antibody, T and Abs dimer, Fv, scFv, dsFv, ds-scFv, Fd, linear antibody, minibody, diabody, bispecific antibody fragment, bibody, tribody, sc-diabody, kappa (. lamda.) body, BiTE, DVD-Ig, SIP, SMIP, DART, or antibody analog containing one or more CDRs.

The following table shows the various antibody structures and targets under investigation:

TABLE 1

Antibody structure	Exemplary targets
		scFv	CC49,ERBB2,Ley
Diabodies antibodies	Ley and TAG-72
		Family complex (Affiniody)	ERBB2
Minibody	CEA,ERBB2
		protein-Fc	Angiogenin 1, angiogenin 2, VEGFR1, VEGFR2
Intact IgG	CD20,CD33,EGFR,ERBB2,VEGF
		IgE and IgM	GM2
Drug conjugates	CD30, CD33 and ERBB2
		Supported nanoparticles	A33, EGFR and transferrin
Dual specificity	CD19–CD3,EPCAM–CD3,gp100–CD3

In some embodiments, the targeting moiety comprises an ATWLPPR polypeptide of VEGFR, a thrombospondin-1 mimetic, an CDCRGDCFCG (cyclic) polypeptide, an SCH221153 fragment, an NCNGRC (cyclic) polypeptide, a CTTHWGFTLC polypeptide, a CGNKRTRGC polypeptide (LyP-1), octreotide, vapreotide, lanreotide, a C-3940 polypeptide, dabigatran, leprosy, norrexed, or cetrorelix.

In some embodiments, the targeting moiety comprises folic acid or a derivative thereof.

In recent years, research on folic acid has been greatly advanced. Folate is a small molecule vitamin essential for cell division. Tumor cell division is abnormal and Folate Receptors (FR) are highly expressed on the surface of tumor cells to capture folate sufficient to support cell division.

The data show that FR expression is 20-fold to 200-fold higher in tumor cells than in normal cells. The expression rate of FR in various malignancies is: 82% in ovarian cancer, 66% in non-small cell lung cancer, 64% in kidney cancer, 34% in colon cancer, and 29% in breast cancer (Xia W, Low PS. late-targeted therapies for cancer. J Med chem.2010;14;53 (19): 6811-24). The expression rate of FA is positively correlated with the malignancy of epithelial tumor infiltration and metastasis. FA enters cells through FR-mediated endocytosis, and FA forms an FA complex with the drug entering the cells through its carboxyl group. Under acidic (pH 5) conditions, FR separates from FA, and FA releases the drug into the cytoplasm.

Clinically, this system can be used to deliver drugs that selectively attack tumor cells. Folic acid has a small molecular weight, is not immunogenic and has high stability, and synthesis of folic acid is inexpensive. More importantly, the chemical coupling between the drug and the carrier is simple, and therefore, the construction of a drug delivery system for cancer treatment using FA as a targeting molecule has become a hot spot of research. Currently, EC145 (FA chemotherapeutic drug conjugate compounds) in clinical trials can effectively attack cancer cells (Prible P and Edelman MJ. EC145: a novel targeted agent for the lung. expert Opin. investig. drugs (2012)21:755 Ack 761).

In some embodiments, the targeting moiety comprises an extracellular domain (ECD) or soluble forms of PD-1, CTLA4, CD47, BTLA, KIR, TIM3,4-1BB, and LAG3, a full-length partial surface ligand amphiregulin, betacellulin, EGF, ephrin, Epigen, epithelial regulatory protein, IGF, neuregulin, TGF, TRAIL, or VEGF.

In some embodiments, the targeting moiety comprises a particle (targeting particle), preferably a nanoparticle, optionally a targeting nanoparticle linked to a targeting molecule that can specifically bind or preferentially bind to a target. In some embodiments, the targeting particle itself directs the compounds of the invention (e.g., by enrichment in tumor cells or tumor tissue) without the need for additional targeting molecules attached thereto.

By "nanoparticle" herein is meant any particle with a diameter of less than 1000 nm. In some embodiments, the therapeutic agent and/or targeting molecule may be bound to a polymer matrix. In some embodiments, the targeting molecule can be covalently bound to the surface of the polymer matrix. In some embodiments, the covalent binding is mediated by a linker. In some embodiments, the therapeutic agent can be bound to the surface of the polymer matrix, encapsulated within the polymer matrix, surrounded by the polymer matrix, and/or dispersed throughout the polymer matrix. U.S. patent No. 8,246,968, the entire contents of which are incorporated herein by reference.

In general, the nanoparticles of the present invention comprise any type of particle. Any particle may be used according to the present invention. In some embodiments, the particles are biodegradable and biocompatible. In general, biocompatible materials are non-toxic to cells. In some embodiments, a substance is considered biocompatible if addition of the substance to a cell produces a result that is less than a certain threshold for cell death. In some embodiments, a substance is considered biocompatible if its addition to a cell does not induce side effects. In general, a biodegradable substance is a substance that degrades under physiological conditions over a therapeutically relevant period of time (e.g., weeks, months, or years). In some embodiments, the biodegradable substance is a substance that can be broken down by cellular mechanisms. In some embodiments, the biodegradable substance is a substance that can be broken down by a chemical process. In some embodiments, the particles are biocompatible and biodegradable materials. In some embodiments, the particles are biocompatible materials, but not biodegradable materials. In some embodiments, the particles are biodegradable, but not biocompatible.

In some embodiments, the particle size of the particles is greater than the renal excretion limit (e.g., particles greater than 6nm in diameter). In some embodiments, the particle size is a size sufficient to avoid clearance from the blood stream by the liver (e.g., particles less than 1000nm in diameter). In general, the physiochemical properties of the particles should allow the targeted particles to circulate in the plasma for long periods of time by reducing renal excretion and hepatic clearance.

In general, it is desirable to use a population of particles that are relatively uniform in size, shape, and/or composition, such that each particle has similar properties. For example, at least 80% of the particles, at least 90% of the particles or at least 95% of the particles have a diameter or largest dimension which is plus or minus 5%, 10% or 20% of the average diameter or largest dimension. In some embodiments, the size, shape, and/or composition of the population of particles can be non-uniform.

A variety of different particles may be used in accordance with the present invention. In some embodiments, the particles are spherical or spheroidal. In some embodiments, the particles are spherical or spheroidal. In some embodiments, the particles are flat or plate-like. In some embodiments, the particles are cubic or cube-like. In some embodiments, the particles are oval or elliptical. In some embodiments, the particles are cylindrical, conical, or pyramidal.

In some embodiments, the particles are microparticles (e.g., microspheres). In general, "microparticles" refers to any particle having a diameter of less than 1000 μm. In some embodiments, the particle is a picoparticle (e.g., a microsphere). In general, "pico particles" refers to any particle with a diameter of less than 1 nm. In some embodiments, the particle is a liposome. In some embodiments, the particles are micelles.

The particles may be solid or hollow and may comprise one or more layers (e.g., nanoshells, nanorings). In some embodiments, each layer has a unique composition and unique properties relative to the other layers. For example, the particles may have a core/shell structure, wherein the core is one layer and the shell is another layer. The particles may comprise a plurality of different layers. In some embodiments, one layer may be fully crosslinked, another layer is not fully crosslinked, and so forth. In some embodiments, one, several, or all of the different layers may comprise one or more therapeutic or diagnostic agents to be delivered. In some embodiments, one layer contains the agent to be delivered, another layer does not contain the agent to be delivered, and so forth. In some embodiments, each individual layer contains a different agent or collection of agents to be delivered.

In some embodiments, the particles are porous, which means that the particles comprise pores or channels, which are typically smaller in size than the particles. For example, the particles may be porous silica particles, e.g., mesoporous silica nanoparticles, or the particles may have a mesoporous silica coating (Lin et al, 2005, j.am. chem.soc.,17: 4570). The particles may have pores with a diameter of about 1nm to about 50nm, for example, pores with a diameter of about 1nm to 20 nm. About 10% to 95% of the volume of the particles may be constituted by voids within the pores or channels.

The particles may have a coating. For example, if the particles contain a substance that is toxic to cells, the use of a biocompatible coating may be advantageous. Suitable coating materials include, but are not limited to: natural proteins such as Bovine Serum Albumin (BSA), biocompatible hydrophilic polymers such as polyethylene glycol (PEG) or PEG derivatives, phospholipids- (PEG), silica, lipids, polymers, carbohydrates such as dextran, other nanoparticles that can be conjugated to the nanoparticles of the present invention, and the like. The coating may be applied or assembled in a variety of ways, such as dipping, using layer-by-layer techniques, self-assembly, conjugation, and the like. Self-assembly refers to the process of spontaneously assembling higher-order structures, which relies on the natural attraction of the components (e.g., molecules) of the higher-order structures to each other. This process typically occurs through random movement of molecules and formation of bonds based on size, shape, composition, or chemical properties.

Examples of polymers include polyolefins (e.g., polyethylene), polycarbonates (e.g., poly (1, 3-dioxan-2-one)), polyanhydrides (e.g., poly (sebacic anhydride)), polyhydroxy acids (e.g., poly (beta-hydroxyalkanoate)), polyfumarates, polycaprolactones, polyamides (e.g., polycaprolactam), polyacetal resins, polyethers, polyesters (e.g., polylactic acid, polyglycolide), poly (orthoesters), polyvinyl alcohols, polyurethanes, polyphosphazenes, polyacrylates, polymethacrylates, polycyanoacrylates, polyureas, polystyrenes, and polyamines. In some embodiments, polymers according to the present invention include polymers that have been approved by the U.S. Food and Drug Administration (FDA) for use in the human body according to 21c.f.r. § 177.2600, including but not limited to: polyesters (e.g., polylactic acid, polyglycolic acid, poly (lactic-co-glycolic acid), polycaprolactone, polygluvalactone, poly (1, 3-dioxan-2-one)), polyanhydrides (e.g., poly (sebacic anhydride)), polyethers (e.g., polyethylene glycol), polyurethanes, polymethacrylates, polyacrylates, and polycyanoacrylates.

In some embodiments, the particles can be non-polymeric particles (e.g., metal particles, quantum dots, ceramic particles, polymers containing inorganic materials, bone-derived materials, bone substitutes, viral particles, etc.). In some embodiments, the therapeutic or diagnostic agent to be delivered may be bound to the surface of such non-polymeric particles. In some embodiments, the non-polymeric particles are aggregates of non-polymeric constituents, e.g., aggregates of metal atoms (e.g., gold atoms). In some embodiments, the therapeutic or diagnostic agent to be delivered can be bound to the surface of and/or encapsulated within, surrounded by, and/or dispersed throughout the aggregate of non-polymeric ingredients.

The particles (e.g., nanoparticles, microparticles) can be prepared using any method known in the art. For example, the granular dosage form may be formed by the following methods, as well as other methods well known to those of ordinary skill in the art: such as nano-precipitation, flow focusing fluidic channels, spray drying, single and double emulsion solvent evaporation, solvent extraction, phase separation, milling, micro-emulsion manipulation, microfabrication, nanofabrication, sacrificial layers, simple and complex coacervation. Alternatively or additionally, aqueous and organic solvent synthesis methods for monodisperse semiconductor nanoparticles, conductive nanoparticles, magnetic nanoparticles, organic nanoparticles and other nanoparticles have been described (Pellegrino et al, 2005, Small,1:48; Murray et al, 2000, Ann. Rev. Mat. Sci.,30:545; and Trindade et al, 2001, chem. Mat.,13: 3843).

Methods of preparing microparticles for delivery of encapsulated agents are described in the literature (see, e.g., Doubrow, eds. "Microcapsules and Nanoparticles in Medicine and Pharmacy," CRC Press, Boca Raton,1992; Mathiowitz et al, 1987, J.Control.Release,5:13; Mathiowitz et al, 1987, Reactive Polymers: 275; and Mathiowitz et al, 1988, J.appl.Polymer Sci.,35: 755).

In some embodiments, the targeting moiety comprises a nucleic acid targeting moiety.

In general, a nucleic acid targeting moiety is any polynucleotide that binds a component (target) associated with an organ, tissue, cell, extracellular matrix component, and/or intracellular compartment.

In some embodiments, the nucleic acid targeting moiety is an aptamer.

Aptamers are generally polynucleotides that bind to a specific target structure associated with a specific organ, tissue, cell, extracellular matrix component, and/or intracellular compartment. In general, the targeting function of aptamers is based on the three-dimensional structure of the aptamers. In some embodiments, binding of an aptamer to a target is typically mediated by an interaction between the two-dimensional and/or three-dimensional structures of both the aptamer and the target. In some embodiments, the binding of an aptamer to a target is not only based on the base sequence of the aptamer, but also depends on the three-dimensional structure of the aptamer and/or the target. In some embodiments, aptamers bind to their target through Watson-Crick complementary base pairing that is hindered by structures (e.g., hairpin loops) that disrupt base pairing.

In some embodiments, the nucleic acid targeting moiety is spiegelmers (PCT publications WO98/08856, WO02/100442, and WO 06/117217). In general, spiegelmers are synthetic mirror image nucleic acids that can specifically bind to a target (i.e., mirror image aptamers). spiegelmers are characterized by the following structural features: the structural features make them less susceptible to exo-and endo-nucleases.

One of ordinary skill in the art will appreciate that any nucleic acid targeting moiety capable of specifically binding to a target (e.g., aptamers or spiegelmers) may be used in accordance with the present invention. In some embodiments, the nucleic acid targeting moieties to be used according to the invention can target markers associated with diseases, disorders and/or conditions. In some embodiments, the nucleic acid targeting moieties to be used according to the invention can target cancer-associated targets. In some embodiments, the nucleic acid targeting moiety to be used according to the present invention may target a tumor marker. Any type of cancer marker and/or any tumor marker can be targeted using the nucleic acid targeting moieties according to the invention. For example, the nucleic acid targeting moiety can target a marker associated with prostate cancer, lung cancer, breast cancer, colorectal cancer, bladder cancer, pancreatic cancer, endometrial cancer, ovarian cancer, bone cancer, esophageal cancer, liver cancer, stomach cancer, brain tumors, cutaneous melanoma, and/or leukemia.

Nucleic acids of the invention (including nucleic acid targeting moieties and/or functional RNAs to be delivered, e.g., RNAi-inducing entities, ribozymes, trnas, and the like, described in further detail below) can be prepared according to any available technique, including but not limited to: chemical synthesis, enzymatic or chemical cleavage of longer precursors, and the like. Methods for synthesizing RNA are known in the art (see, e.g., Gait, M.J. (eds.) Oligonuclotide synthesis: a practical approach, Oxford [ Oxfordshire ], Washington, D.C.: IRL Press,1984; and Herdevijn, P. (eds.) Oligonuclotide synthesis: Methods and applications, Methods in molecular biology, v.288(Clifton, N.J.) Totowa, N.J.: Humana Press, 2005).

The nucleic acid forming the nucleic acid targeting moiety can comprise a naturally occurring nucleoside, a modified nucleoside, a naturally occurring nucleoside having a hydrocarbon linker (e.g., an alkene) or a polyether linker (e.g., a PEG linker) interposed between one or more nucleosides, a modified nucleoside having a hydrocarbon linker or a PEG linker interposed between one or more nucleosides, or a combination thereof. In some embodiments, the nucleotides or modified nucleotides of the nucleic acid targeting moiety can be substituted with a hydrocarbon linker or a polyether linker, provided that the binding affinity and selectivity of the nucleic acid targeting moiety is not substantially due to the substitution (e.g., the dissociation constant of the nucleic acid targeting moiety for the target should not be greater than about 1 x10^-3M) is decreased.

It is known to the person skilled in the art that the nucleic acids according to the invention may comprise all types of nucleotides found in naturally occurring nucleic acids or may comprise one or more nucleotide analogs or have a structure which differs from the structure of naturally occurring nucleic acids. U.S. patent nos. 6,403,779, 6,399,754, 6,225,460, 6,127,533, 6,031,086, 6,005,087, 5,977,089, the references therein disclosing a variety of specific nucleoside analogs and modifications that can be used. See crook, S. (eds.) Antisense Drug Technology: Principles, Strategies, and Applications (first edition), Marcel Dekker, ISBN:0824705661, first edition (2001) and references therein. For example, 2' -modifications include halo, alkoxy, and allyloxy. In some embodiments, the 2' -OH group is substituted with a group selected from: h, OR, R, halogen, SH, SR, NH2, NHR, NR2 OR CN, wherein R is C1-C6 alkyl, alkenyl, OR alkynyl, and halogen is F, Cl, Br OR I. Examples of modified linkages include phosphorothioate and 5' -N-phosphoramidite linkages.

Nucleic acids comprising a variety of different nucleotide analogs, modified backbones, or non-naturally occurring internucleoside linkages can be used according to the invention. Nucleic acids of the invention may include natural nucleosides (i.e., adenosine, thymidine, guanosine, cytidine, uridine, deoxyadenosine, deoxythymidine, deoxyguanosine, deoxycytidine) or modified nucleosides. Examples of modified nucleotides include base-modified nucleosides (e.g., cytarabine (aracytidine), myonucleosides, isoguanosine, pinocyanine (nebularine), pseudouridine, 2, 6-diaminopurine, 2-aminopurine, 2-thiothymidine, 3-deaza-5-azacytidine, 2' -deoxyuridine, 3-nitropyrrole, 4-methylindole, 4-thiouridine, 4-thiothymidine, 2-aminoadenosine, 2-thiothymidine, 2-thiouridine, 5-bromocytidine, 5-iodouridine, myonucleosides, 6-azauridine, 6-chloropurine, 7-deazaadenosine, 7-deazaguanosine, 8-azaadenosine, 8-azidoadenosine, benzimidazole, M1-methyladenosine, inosine, isoguanosine, noruridine, pseudouridine, 2-thiouridine, 2-iodouridine, 5-iodouridine, inosine, Pyrrolopyrimidine, 2-amino-6-chloropurine, 3-methyladenosine, 5-propynylcytidine, 5-propynyluridine, 5-bromouridine, 5-fluorouridine, 5-methylcytidine, 7-deazaadenosine, 7-deazaguanosine, 8-oxyadenosine, 8-oxoguanosine, O (6) -methylguanine and 2-thiocytidine), chemically or biologically modified bases (e.g., methylated bases), modified sugars (e.g., 2 ' -fluororibose, 2 ' -aminoribose, 2 ' -azidoribose, 2 ' -O-methylribose, L-enantiomeric nucleoside arabinose, and hexoses), modified phosphate groups (e.g., phosphorothioate and 5 ' -N-phosphoramidite linkages), and combinations thereof. Natural nucleotide monomers and modified nucleotide monomers for chemical synthesis of nucleic acids are readily available. In some cases, nucleic acids containing these modifications exhibit improved properties relative to nucleic acids consisting only of naturally occurring nucleotides. In some embodiments, the nucleic acid modifications described herein are used to reduce and/or prevent nuclease (e.g., exonuclease, endonuclease, etc.) digestion. For example, the structure of a nucleic acid can be stabilized by including nucleotide analogs at the 3' end of one or both strands to reduce digestion.

The modified nucleic acid need not be consistently modified along the entire length of the molecule. Different nucleotide modifications and/or backbone structures may be present at various positions of the nucleic acid. It will be appreciated by those of ordinary skill in the art that nucleotide analogs or other modifications can be located anywhere in the nucleic acid that renders the function of the nucleic acid substantially unaffected. For example, the modification may be located anywhere on the nucleic acid targeting moiety that does not substantially affect the ability of the nucleic acid targeting moiety to specifically bind to a target. The modified region may be located at the 5 'end and/or the 3' end of one or both strands. For example, nucleic acid targeting moieties have been modified as follows: about 1 to 5 residues at the 5 'end and/or 3' end of either strand of the modified nucleic acid targeting moiety are nucleotide analogs and/or have backbone modifications. The modification may be a 5 'or 3' terminal modification. One or both nucleic acid strands may comprise at least 50% unmodified nucleotides, at least 80% unmodified nucleotides, at least 90% unmodified nucleotides or 100% unmodified nucleotides.

For example, nucleic acids according to the invention may comprise modifications to sugars, nucleosides, or internucleoside linkages, such as those described in U.S. patent application publication nos. 2003/0175950, 2004/0192626, 2004/0092470, 2005/0020525 and 2005/0032733. The invention includes the use of any nucleic acid having any one or more of the modifications described herein. For example, various terminal conjugates (e.g., lipids (e.g., cholesterol), lithocholic acid, lauric acid (aluric acid), long-chain branched alkyl) have been reported to improve cellular uptake. For example, analogs and modifications can be detected using any suitable test method known in the art, thereby selecting those analogs and modifications that result in improved delivery of a therapeutic or diagnostic agent, improved specific binding of a targeting portion of a nucleic acid to a target, and the like. In some embodiments, a nucleic acid according to the invention can include one or more non-natural nucleoside linkages. In some embodiments, one or more internal nucleotides located at the 3 'end, the 5' end, or both the 3 'end and the 5' end of the nucleic acid targeting moiety are inverted to generate a linkage, such as a3 '-3' linkage or a 5 '-5' linkage.

In some embodiments, the nucleic acid according to the invention is not synthetic, it is a naturally occurring entity that has been isolated from its natural environment.

Novel nucleic acid targeting moieties can be designed using any method (see, e.g., U.S. Pat. Nos.: 6,716,583;6,465,189;6,482,594;6,458,543;6,458,539;6,376,190;6,344,318;6,242,246;6,184,364;6,001,577;5,958,691;5,874,218;5,853,984;5,843,732;5,843,653;5,817,785;5,789,163;5,763,177;5,696,249;5,660,985;5,595,877;5,567,588 and 5,270,163; and U.S. patent application publications: 2005/0069910, 2004/0072234,2004/0043923,2003/0087301,2003/0054360 and 2002/0064780). The present invention provides a method for designing novel nucleic acid targeting moieties. The invention also provides a method for isolating or identifying novel nucleic acid targeting moieties from a mixture of candidate nucleic acid targeting moieties.

Nucleic acid targeting moieties that bind to proteins, carbohydrates, lipids, and/or nucleic acids can be designed and/or identified. In some embodiments, the nucleic acid targeting moiety can be designed and/or identified for use in a complex of the invention that binds to a protein and/or a characteristic portion thereof, e.g., a tumor marker, an integrin, a cell surface receptor, a transmembrane protein, an intercellular protein, an ion channel, a membrane transporter, an enzyme, an antibody, a chimeric protein, and the like. In some embodiments, the nucleic acid targeting moiety can be designed and/or identified for use in the complexes of the invention in combination with carbohydrates and/or their characteristic moieties, e.g., glycoproteins, saccharides (e.g., monosaccharides, disaccharides, and polysaccharides), glycocalyx (i.e., a carbohydrate-enriched peripheral region on the outer surface of most eukaryotic cells), and the like. In some embodiments, the nucleic acid targeting moiety can be designed and/or identified for use in a complex of the invention in combination with a lipid and/or a characteristic moiety thereof, e.g., an oil, a saturated fatty acid, an unsaturated fatty acid, a glyceride, a hormone, a steroid (e.g., cholesterol, bile acid), a vitamin (e.g., vitamin E), a phospholipid, a sphingolipid, a lipoprotein, and the like. In some embodiments, nucleic acid targeting moieties can be designed and/or identified for use in complexes of the invention that bind to nucleic acids and/or their characteristic portions, e.g., DNA nucleic acids, RNA nucleic acids, modified DNA nucleic acids, modified RNA nucleic acids, and nucleic acids comprising any combination of DNA, RNA, modified DNA, and modified RNA, and the like.

Any available method can be used to design and/or identify nucleic acid targeting moieties (e.g., aptamers or spiegelmers). In some embodiments, the nucleic acid targeting moiety is designed and/or identified by identifying the nucleic acid targeting moiety from a candidate mixture of nucleic acids. Exponential enrichment ligand systemic evolution (SELEX) or its improved methods are common methods for identifying target-binding nucleic acid targeting moieties from a mixture of candidate nucleic acids.

A nucleic acid targeting moiety that selectively binds to any target can be isolated by the SELEX method or a modification thereof, provided that the target can be used as a target in the SELEX method.

Connecting body

In general, the compounds of the invention comprise a linker connecting the targeting moiety and the activating moiety. However, some compounds do not contain a linker, and the activating moiety and targeting moiety are directly linked.

A "linker" herein refers to a moiety that chemically links a first molecule to a second molecule. In the linkers of the invention, the linkage may be cleaved, thereby releasing the biologically active form of the first and/or second molecule. A preferred example of a linker is a moiety comprising a chemical bond that is stable at neutral pH conditions but susceptible to cleavage at lower pH conditions. Specifically, examples of preferred linkers are moieties comprising a chemical bond that is stable at pH 7 to 8 but is easily cleaved at pH 4 to 6. Another example of a linker is a moiety comprising a chemical bond that is susceptible to cleavage in the presence of an enzyme. Preferred examples of such enzyme-sensitive linkers are peptides comprising the recognition sequence of an endosomal peptidase. Another example of a linker is a redox potential sensitive linker that is stable under low reduction potential (e.g., low concentrations of thiol or glutathione) conditions but that cleaves under high reduction potential (e.g., high concentrations of thiol or glutathione) conditions. Preferred examples of such redox potential sensitive linkers include disulfides and sulfenamides. In particular, preferred examples include substituted aryl-alkyl disulfides in which the aryl group is substituted with a sterically demanding and electron-withdrawing or electron-donating substituent to control the sensitivity of the disulfide bond to react with thiols. Another example of a linker is a moiety comprising a chemical bond that is susceptible to cleavage upon exposure to radiation. A preferred example of these radiation-sensitive linkers is 2-nitrobenzyl ether, which cleaves after exposure to light. Specifically, preferred examples of these linkers are the following moieties: this moiety masks the biological activity of one of the two linked molecules before the linkage is cleaved.

In some embodiments, the compounds of the present invention comprise a linker selected from the group consisting of: hydrazine groups, polypeptides, disulfide groups and thioether groups.

By "hydrazine group" or "hydrazine linker" or "self-cyclizing hydrazine linker" herein is meant a linker moiety that can undergo a cyclization reaction and form one or more rings upon changing conditions (e.g., a change in pH). When attached, the hydrazine group is converted to a hydrazone. Such attachment may occur, for example, by reaction with a ketone group at the L4 moiety. Thus, the term hydrazine linker may also be used to describe the linker of the invention, as this conversion to hydrazone occurs after the linkage.

As used herein, a "five-membered hydrazine linker" refers to a hydrazine-containing moiety that undergoes a cyclization reaction and forms one or more five-membered rings upon a change in conditions (e.g., a change in pH). Alternatively, the five-membered linker can be similarly described as a five-membered hydrazine linker.

A "six-membered hydrazine linker" herein refers to a hydrazine-containing molecular moiety that undergoes a cyclization reaction and forms one or more six-membered rings upon a change in conditions (e.g., a change in pH). The six-membered linker can be similarly described as a six-membered hydrazine linker.

As used herein, "cyclization reaction" refers to cyclization of a peptide, hydrazine, or disulfide linker, which "cyclization reaction" means cyclization of the linker to form a ring and initiate separation of the drug-ligand complex. The rate of cyclization can be measured off-site and is completed when at least 90%, 95%, or 100% of the product is formed.

In some embodiments, the compounds of the invention comprise a linker region located between the targeting moiety and the activating moiety, and the linker is cleavable by a lytic agent present in the intracellular environment (e.g., lysosomes or endosomes or fovea). The linker can be, for example, a peptidyl linker that is cleaved by a cellular endopeptidase or protease (including, but not limited to, lysosomal proteases or endosomal proteases). Typically, the peptidyl linker is at least two amino acids in length or at least three amino acids in length. Lytic agents may include cathepsin B, cathepsin D, and plasmin, all of which are known to hydrolyze dipeptide drug derivatives, resulting in the release of active drug inside target cells (see, e.g., Dubowchik and Walker,1999, pharm. The most typical linker is a peptidyl linker, which can be cleaved by an enzyme present in the target cell or tissue. For example, a peptidyl linker (e.g., a Phe-Leu or (Gly-Phe-Leu-Gly) linker) can be used that is cleavable by a thiol-dependent protease cathepsin-B, which is highly expressed in cancer tissues. Other such linkers are described, for example, in U.S. Pat. No.6,214,345. In some embodiments, the peptidyl linker cleavable by an intracellular protease is a Val-Cit linker or a Phe-Lys linker (see, e.g., U.S. Pat. No.6,214,345, which describes the synthesis of doxorubicin with a Val-Cit linker). One advantage of using intracellular proteolytic release of the therapeutic agent is that the therapeutic agent is generally weakened upon conjugation and the serum stability of the conjugate is generally higher.

In some embodiments, the cleavable linker is pH sensitive, i.e., sensitive to certain pH conditions and undergoes hydrolysis. Typically, the pH sensitive linker is hydrolyzable under acidic conditions. For example, acid-labile linkers that are hydrolyzable in lysosomes (e.g., hydrazones, semicarbazones, thiosemicarbazones, cis-aconitamides, orthoesters, acetals, ketals, etc.) may be used, see, e.g., U.S. Pat. No. 5,122,368, No. 5,824,805, No. 5,622,929, Dubowchik and Walker,1999, pharm. therapeutics83:67-123; Neville et al, 1989, biol. chem.264: 14653-14661. These linkers are relatively stable at neutral pH conditions, e.g., those in the blood, but are unstable at pH conditions below 5.5 or 5.0 (about the pH of lysosomes). In some embodiments, the hydrolyzable linker is a thioether linker (e.g., a thioether linked to the therapeutic agent via an acylhydrazone bond, see, e.g., U.S. patent No. 5,622,929).

In other embodiments, the linker is cleavable under reducing conditions (e.g., a disulfide linker). A variety of disulfide linkers are known In the art, including, for example, those that can be formed using SATA (N-succinimidyl-5-acetylthioacetate), SPDP (N-succinimidyl-3- (2-pyridyldithio) propionate), SPDB (N-succinimidyl-3- (2-pyridyldithio) butyrate) and SMPT (N-succinimidyl-oxycarbonyl- α -methyl- α - (2-pyridyldithio) toluene), SPDB and SMPT (see, e.g., Thorpe et al, 1987, Cancer Res.47: 5924. cndot. 5931; Wawrzynczak et al, In Immunoconjugates: Antibody Conjugates In radiodiagnosis and Therapy of Cancer (edited by C.W.Vogel), Oxford U. published, 1987. see also U.S. Pat. No. 4,880,935).

In other embodiments, the linker is a malonate linker (Johnson et al, 1995, Anticancer Res.15: 1387-93), a maleimidobenzoyl linker (Lau et al, 1995, Bioorg-Med-chem.3(10): 1299-1304) or a 3' -N-amide analog (Lau et al, 1995, Bioorg-Med-chem.3(10): 1305-12).

Generally, the linker is substantially insensitive to the extracellular environment. As used herein in the context of the portion of the linker, "substantially insensitive to the extracellular environment" means that in a sample of a compound of the invention, when the compound of the invention is present in the extracellular environment (e.g., in plasma), no more than about 20% of the linker is cleaved, typically no more than about 15% of the linker is cleaved, more typically no more than about 10% of the linker is cleaved, even more typically no more than about 5% of the linker is cleaved, no more than about 3% of the linker is cleaved, or no more than about 1% of the linker is cleaved. For example, whether a linker is substantially insensitive to the extracellular environment can be determined by separately incubating (a) a compound of the invention ("compound sample") and (b) an equimolar amount of unconjugated antibody or therapeutic agent ("control sample") with plasma for a predetermined period of time (e.g., 2 hours, 4 hours, 8 hours, 16 hours, or 24 hours) and then comparing the amount of unconjugated antibody or therapeutic agent present in the compound sample to the amount of unconjugated antibody or therapeutic agent present in the control sample (e.g., as measured by high performance liquid chromatography).

In other, non-mutually exclusive embodiments, the linker facilitates cell internalization. In some embodiments, when the linker is coupled to the activating moiety, the linker facilitates cell internalization. In other embodiments, when the linker is coupled to both the targeting moiety and the activating moiety, the linker facilitates cellular internalization.

Various linkers that can be used in the compositions and methods of the invention are described in WO2004010957 (entitled "Drug Conjugates and the same Use for Treating Cancer, An Autoimmumne Disease or An Infectious Disease") and US20120141509A1 and US20120288512A1 (the disclosure of which is incorporated herein by reference).

In some embodiments, the linker unit has the general formula:

-Ta-Ww-Yy-

wherein, -T-is a scaffold unit, a is 0 or1, -W-is each independently an amino acid unit, W is independently an integer from 2 to 12, -Y-is a spacer unit, Y is 0,1 or 2.

Stand unit

When a scaffold unit (-T-) is present, the scaffold unit connects the targeting moiety to an amino acid unit (-W-). Useful functional groups that may be present on the targeting moiety (e.g., antibody) either naturally or by chemical manipulation include, but are not limited to: sulfhydryl, amino, hydroxyl, anomeric hydroxyl of carbohydrate and carboxyl. Suitable functional groups are mercapto and amino. Sulfhydryl groups can be generated by reducing intramolecular disulfide bonds of antibodies. Alternatively, the sulfhydryl group may be generated by reaction of the amino group of the lysine group of the antibody with 2-iminothiolane (Traut's reagent) or other sulfhydryl-generating reagent. In some embodiments, the antibody is a recombinant antibody and is designed with one or more lysines. In other embodiments, the recombinant antibody is designed with an additional sulfhydryl group, e.g., an additional cysteine.

In some embodiments, the scaffold unit forms a chemical bond with a sulfur atom of the antibody. The sulfur atom may be derived from a thiol group (-SH) of the reduced antibody (A). Representative scaffold units of these embodiments are described in brackets of general formulae (IIa) and (IIb), wherein A-, -W-, -Y-, -D, W, and Y are as defined above, and R¹Selected from: -C₁-C₁₀Olefin-, -C₃-C₈-carbocyclic ring-, -O- (C)₁-C₈Alkyl) -, -arylene-, -C₁-C₁₀Alkene-arylene-, -arylene-C₁-C₁₀-olefin-, -C₁-C₁₀-olefin- (C)₃-C₈Carbocycle) -, - (C)₃-C₈Carbocyclic ring) -C₁-C₁₀Olefin-, -C₃-C₈-heterocycle-, -C₁-C₁₀-olefin- (C)₃-C₈Heterocycle) -, - (C)₃-C₈Heterocycle) -C₁-C₁₀Olefin-, - (CH)₂CH₂O) r-and- (CH)₂CH₂O)r-CH₂-, and r is an integer of 1 to 10.

An exemplary stent unit is R¹Is- (CH)₂)₅-a scaffold unit of formula (IIa):

another exemplary stent unit is R¹Is- (CH)₂CH₂O)r-CH₂-and a scaffold unit of formula (IIa) when r is 2:

another exemplary stent unit is R¹Is- (CH)₂)₅-a scaffold unit of general formula (IIb):

in some other specific embodiments, the scaffold unit is linked to the antibody unit (a) by a disulfide bond between a sulfur atom of the antibody unit and a sulfur atom of the scaffold unit. Representative scaffold units of this embodiment are described in brackets of general formula (III), wherein R is¹A-, -W-, -Y-, -D, W and Y are as defined above.

In other embodiments, the reactive group of the scaffold comprises a reactive site that can react with an amino group of an antibody. The amino group may be arginine or lysine. Suitable amine reaction sites include, but are not limited to: activated esters (e.g., succinimidyl ester, 4-nitrophenyl ester, pentafluorophenyl ester), anhydrides, acid chlorides, sulfonyl chlorides, isocyanates, and isothiocyanates. Representative scaffold units of these embodiments are described in brackets of general formulae (IVa) and (IVb), wherein R¹A-, -W-, -Y-, -D, W and Y are as described above.

In another aspect, the reactive functional group of the scaffold comprises a reactive site that reacts with a modified carbohydrate group that may be present on the antibody. In some embodiments, the antibody is glycosylated enzymatically, thereby generating carbohydrate groups. Carbohydrates can be mildly oxidized by agents such as sodium periodate and the resulting oxidized carbohydrate can be reacted with a compound containing a carbonyl unit such as a hydrazide, oxime, reactive amine, hydrazine, thiosemicarbazide, hydrazine carboxylate, and aryl hydrazide (e.g., Kaneko et al, 1991, Bi)Functional groups such as those described in oconjugate Chem2: 133-41). Representative scaffold units of this embodiment are described in brackets of general formulas (Va) to (Vc), where R is¹A-, -W-, -Y-, -D, W and Y are as described above.

Amino acid unit

The amino acid unit (-W-) links the scaffold unit (-T-) to the spacer unit (-Y-) if the spacer unit is present, and the amino acid unit links the scaffold unit to a cytotoxic or cytostatic agent (activating moiety, D) if the spacer unit is not present. -Ww-is a dipeptide, tripeptide, tetrapeptide, pentapeptide, hexapeptide, heptapeptide, octapeptide, nonapeptide, decapeptide, undecapeptide, or dodecapeptide unit. each-W-unit independently has the formula as set forth in the following brackets, and W is an integer from 2 to 12.

Wherein R is²Is hydrogen, methyl, isopropyl, isobutyl, sec-butyl, benzyl, p-hydroxybenzyl, — CH₂OH、—CH(OH)CH₃、—CH₂CH₂SCH₃、—CH₂CONH₂、—CH₂COOH、—CH₂CH₂CONH₂、—CH₂CH₂COOH、—(CH₂)₃NHC(＝NH)NH₂、—(CH₂)₃NH₂、—(CH₂)₃NHCOCH₃、—(CH₂)₃NHCHO、—(CH₂)₄NHC(＝NH)NH₂,—(CH₂)₄NH₂、—(CH₂)₄NHCOCH₃、—(CH₂)₄NHCHO、—(CH₂)₃NHCONH₂、—(CH₂)₄NHCONH₂、—CH₂CH₂CH(OH)CH₂NH₂2-pyridylmethyl-, 3-pyridylmethyl-, 4-pyridylmethyl-, phenyl, cyclohexyl,

The amino acid units of the linker unit may be enzymatically cleaved by means of an enzyme, including but not limited to, tumor-associated proteases, to release an activating moiety (-D), which upon release is protonated in vivo to produce the activating molecule (D).

Exemplary W_wThe units are represented by the general formulae (VI) to (VIII):

wherein R is³And R⁴As shown in the following table:

wherein R is³、R⁴And R⁵As shown in the following table:

wherein R is³、R⁴、R⁵And R⁶As shown in the following table:

suitable amino acid units include, but are not limited to: a unit of the formula (VI), in which R³Is benzyl, R⁴Is- (CH)₂)₄NH₂，R³Is isopropyl and R⁴Is- (CH)₂)₄NH₂Or R is³Is isopropyl and R⁴Is- (CH)₂)₃NHCONH₂. Another suitable amino acid unit is a unit of the formula (VII), in which R³Is benzyl, R⁴Is benzyl and R⁵Is- (CH)₂)₄NH₂. the-Ww-unit can be designed and optimized in its selectivity for enzymatic cleavage by specific tumor-associated proteases. suitable-Ww-units are those whose cleavage is catalyzed by proteases (cathepsin B, cathepsin C and cathepsin D) as well as plasmin.

In some embodiments, -Ww-is a dipeptide, tripeptide or tetrapeptide unit.

At R²、R³、R⁴、R⁵Or R⁶In the absence of hydrogen, R²、R³、R⁴、R⁵Or R⁶The carbon atoms to which they are attached are chiral. And R²、R³、R⁴、R⁵Or R⁶Each carbon atom attached is independently in the (S) or (R) configuration.

In some embodiments, the amino acid unit is a phenylalanine-lysine dipeptide (Phe-Lys or FK linker). In some embodiments, the amino acid unit is a valine-citrulline dipeptide (Val-Cit or VC linker). In some embodiments, the amino acid unit is 5-aminopentanoic acid, homophenylalanine lysine, tetrahydroisoquinoline carboxylate lysine, cyclohexylalanine lysine, isoperidolic acid (isonepecotic acid) lysine, β -alanine lysine, glycine serine valine glutamine, or isoperidolic acid.

The amino acid unit may comprise a natural amino acid. In other embodiments, the amino acid unit can comprise an unnatural amino acid.

Spacer unit

When a spacer unit (-Y-) is present, the spacer unit links the amino acid unit to the drug unit. The spacer elements are of two general classes: self-excising (self-immolative) and non-self-excising. A non self-excising spacer unit is one in which some or all of the spacer unit remains attached to the activating moiety unit following enzymatic cleavage of the amino acid unit in the TM-linker-AM conjugate or the drug linker compound. Examples of non-self-slicing spacer units include, but are not limited to: (glycine-glycine) spacer units and glycine spacer units. When the TM-linker-AM conjugate containing a glycine-glycine spacer or glycine spacer is enzymatically cleaved by a tumor cell-associated protease, a cancer cell-associated protease or a lymphocyte-associated protease, the glycine-drug moiety or glycine-drug moiety is cleaved from the a-T-Ww-. To release AM, a separate hydrolysis reaction should be performed in the target cell, thereby cleaving the glycine-drug unit chemical bond.

In a typical embodiment, -Yy-is p-aminobenzyl ether which may be substituted by Qm, wherein Q is-C₁-C₈Alkyl radical, -C₁-C₈Alkoxy, -halogen, -nitro or-cyano, and m is an integer from 0 to 4.

In some embodiments, the non-self-cleaving spacer unit (-Y-) is-Gly-Gly-.

In some embodiments, the non-self-cleaving spacer unit (-Y-) is-Gly-.

In one embodiment, the AM-linker compound or TM-linker-AM conjugate lacks a spacer unit (y = 0).

Alternatively, TM-linker-AM conjugates containing self-cleaving spacer units may release AM (d) without a separate hydrolysis step. In these embodiments, -Y-is a p-aminobenzyl alcohol (PAB) unit that is linked to-Ww-through the nitrogen atom of the PAB group and is directly linked to-D through a carbonate group, a carbamate group, or an ether group.

Other examples of self-slicing spacer units include, but are not limited to: aromatic compounds that are electronically equivalent to the PAB group, for example, 2-aminoimidazolyl-5-carbinol derivatives (see, e.g., Hay et al, 1999, bioorg. Med. chem. Lett.9: 2237) and ortho-or para-aminobenzyl acetals. Spacer units which are susceptible to cyclisation upon hydrolysis of the amide bond may be used, for example substituted and unsubstituted 4-aminobutanoic acid amides (Rodrigues et al, 1995, Chemistry Biology2: 223), appropriately substituted bicyclo [2.2.1] and bicyclo [2.2.2] ring systems (Storm et al, 1972, J.Amer.chem.Soc.94: 5815) and 2-aminophenylpropionic acid amides (Amsberry et al, 1990, J.org.chem.55: 5867). Elimination of amine-containing drugs substituted alpha to glycine (Kingsbury et al, 1984, J.Med.Chem.27: 1447) is also an example of a strategy for self-excision of the spacer unit, which can be used for TM-linker-AM conjugates.

In an alternative embodiment, the spacer unit is a branched bis (hydroxymethyl) styrene (BHMS) unit, which may be used to combine multiple moieties.

Typical spacer units (-Yy-) are represented by the general formulae (IX) - (XI):

wherein Q is C₁-C₈Alkyl radical, C₁-C₈Alkoxy, halogen, nitro or cyano, and m is an integer of 0 to 4.

In some embodiments, the linker is enzymatically cleavable. In some embodiments, the linker is not enzymatically cleavable.

In some embodiments, the linker is represented by the structure of the following general formula (III):

wherein L is represented by the structure of formula (II):

wherein i is each independently 0 or 1;

j is each independently 0,1, 2,3, 4,5 or 6;

each B is independently alkyl, alkenyl, -O-alkyl, -alkyl-O, -S-alkyl, -alkyl-S, -aryl, heteroaryl, heterocyclyl or peptide, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, - - -alkyl-arylThe group-alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄，--C(O)-R₄，--C(O)-O-R₄，--S-R₄，--S(O)₂-R₄，--NHR₄- - -NH-alkyl-R₄Halogen, - -CN, - -NO₂and-SH, wherein R₄Is alkyl, alkenyl, -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl.

In some embodiments, the linker is represented by the following structures of general formula (V) to general formula (VII):

a, B, i and j are as defined above.

Preparation of the Compounds

In general, the activated moieties represented by the structures of formula (I) can be prepared using the synthetic procedures outlined below. In step (1), 4-chloro-3-nitroquinoline of the formula A is reacted with a compound of the formula R₁NH₂To produce 3-nitroquinolin-4-amines of the general formula B. In step 2, the 3-nitroquinolin-4-amine of formula B is reduced to form a quinoline-3-4-diamine of formula C. In step 3, quinoline-3-4-diamines of formula C are reacted with carboxylic acids or their equivalents to form 1H-imidazo [4,5C ] of formula D]Quinoline.

Alternatively, the compounds of general formula (I) may be prepared according to the synthetic methods described in US6,331,539b1, US6,451,810b1, US7,157,452 and US7,301027B2.

On the other hand, the compounds of general formula (Ia) and general formula (Ib) can be prepared by using a linker to link both the targeting moiety and the activating moiety. The linker is bound to the targeting moiety and the activating moiety using its reactive site. In some embodiments, the binding is by formation of a covalent bond between the linker and the targeting and activating moieties. In some embodiments, the reactive site is a nucleophilic group. In some embodiments, the reactive site is an electrophilic group. Nucleophilic groups useful in linkers include, but are not limited to: hydrazide groups, oxime groups, amino groups, hydrazine groups, thiosemicarbazone groups, hydrazine carboxylate groups, and aryl hydrazide groups. Useful electrophilic groups include, but are not limited to: maleimide groups, carbonate groups and haloacetamide groups.

Pharmaceutical dosage form and mode of administration

The invention also relates to a pharmaceutical composition comprising a compound of the invention, or a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable carriers.

The compounds described herein (e.g., addition salts or hydrates thereof) and pharmaceutically acceptable carriers can be delivered to a patient using a variety of different modes or routes of administration. Suitable routes of administration include, but are not limited to: inhalation, transdermal, oral, rectal, transmucosal, intestinal, and parenteral (including intramuscular, subcutaneous, and intravenous injections). Preferably, the compounds of the invention comprising an antibody or antibody fragment as targeting moiety are administered parenterally, more preferably, intravenously.

The term "administering" as used herein is intended to include all manner of delivering a compound, both directly and indirectly, to the desired site of action.

The compounds described herein, or pharmaceutically acceptable salts thereof and/or hydrates thereof, may be administered alone, in combination with other compounds of the present invention and/or in combination with other therapeutic agents in the form of a cocktail. Of course, the choice of therapeutic agent that can be administered in combination with the compounds of the present invention will depend in part on the condition being treated.

For example, when administered to a patient suffering from a disease condition caused by an autoinducer-dependent organism, the compounds of the invention may be administered in the form of a cocktail containing agents useful for treating pain, infections and other conditions, as well as side effects commonly associated with disease. Such agents include, for example, analgesics, antibiotics, and the like.

When administered to a patient undergoing cancer treatment, the compounds may be administered in the form of a cocktail comprising anti-cancer agents and/or complementary potentiators. The compounds may also be administered in the form of a cocktail containing agents that treat the side effects of radiation therapy, e.g., antiemetics, radioprotectants, and the like.

Supplementary potentiating agents which can be administered in combination with the compounds of the present invention include, for example, tricyclic antidepressants (e.g., mipramine (imipramine), desipramine (desipramine), amitriptyline (amitriptyline), clomipramine (clomipramine), trimipramine (trimipramine), doxepin (doxepin), nortriptyline (nortriptyline), protiline (protriptyline), amoxapine (amoxapine) and maprotiline (maprotiline)), non-tricyclic antidepressants (e.g., sertraline (sertraline), trazodone (trazodone) and citalopram (citalopram)), Ca2+ antagonists (e.g., verapamil (verapamil), nifedipine (nifedipine), nitrendipine (nifedipine) and caroverine (triproline)), amphiphilic antagonists (e, e.g., flunixipholidine (oxyphine)), amphiphilic drugs (e, e.g., drugs (triamcinolone (e)), amphiphilic drugs (e, e.g., drugs (triamcinolone (trinitropine, trinine, trinitropine, e), and (e, e (trinitro, buthionine and sulfoximine) and calcium formyltetrahydrofolate.

The active compounds of the present invention are administered as such or in the form of a pharmaceutical composition in which the active compound is mixed with one or more pharmaceutically acceptable carriers, excipients or diluents. The pharmaceutical compositions used according to the invention are generally formulated in a conventional manner using one or more physiologically acceptable carriers comprising excipients and adjuvants, which facilitate processing of the active compounds into preparations which can be used pharmaceutically. The appropriate dosage form depends on the chosen route of administration.

For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the dosage form. Such penetrants are generally known in the art.

For oral administration, the compounds are readily formulated by combining the active compound with pharmaceutically acceptable carriers known in the art. These carriers enable the compounds of the invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries and suspensions, for oral administration to a patient to be treated. Pharmaceutical preparations for oral use can be obtained by: the solid excipients are mixed with the active agent compounds, optionally the resulting mixture is ground, and the mixture of granules is processed, if tablets or dragee cores are desired, with the addition of suitable adjuvants prior to the process. Suitable excipients are, in particular, fillers (e.g. sugars including lactose, sucrose, mannitol or sorbitol), cellulose preparations (e.g. corn starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, methylcellulose, hydroxypropylmethylcellulose, sodium carboxyethylcellulose) and/or polyvinylpyrrolidone (PVP). If desired, disintegrating agents can be added, for example, cross-linked polyvinylpyrrolidone, agar, or alginic acid or a salt thereof (e.g., sodium alginate).

Sugar-coated tablet cores are provided with suitable coatings. To achieve this, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbomer gel, polyethylene glycol and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different active compound dose combinations.

Pharmaceutical preparations for oral use include push-fit capsules made of gelatin and soft, sealed capsules made of gelatin and a plasticizer (e.g., glycerol or sorbitol). Push-fit capsules may contain the active ingredient in admixture with fillers such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, for example, fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. The dosage of all dosage forms for oral administration should be adapted to this mode of administration.

For oral administration, the composition may be administered in the form of tablets or troches formulated in conventional manner.

For administration by inhalation, the compounds used in accordance with the present invention are conveniently delivered in the form of a spray presentation from a pressurized pack or nebulizer, with the use of a suitable propellant (e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas). Where a pressurized spray is used, the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges (e.g., gelatin capsules and cartridges) for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base (e.g., lactose or starch).

The compounds may be formulated for parenteral administration by injection (e.g., bolus injection or continuous infusion). Injection is a preferred method of administration of the compositions of the present invention. Dosage forms for injection may be provided in unit dose form with an added preservative, e.g., in ampoules or in multi-dose containers. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents (formulations) such as suspending, stabilizing and/or dispersing agents, for example, and may incorporate cross-linked polyvinylpyrrolidone, agar or alginic acid or a salt thereof (e.g. sodium alginate).

Pharmaceutical dosage forms for parenteral administration comprise aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils (e.g., sesame oil) or synthetic fatty acid esters (e.g., ethyl oleate or triglycerides) or liposomes. Aqueous injection suspensions may contain the following: this material increases the viscosity of the suspension, for example sodium carboxymethylcellulose, sorbitol or dextran. Optionally, the suspension may also contain suitable stabilizers or agents that increase the solubility of the compound, thereby producing a highly concentrated solution. For injection, the agents of the invention may be formulated as aqueous solutions, preferably as physiologically compatible buffers (e.g., Hanks 'solution, ringer's solution, or physiological saline buffer).

Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile, pyrogen-free water, before use.

The compounds may also be formulated in rectal compositions, for example, in suppositories or retention enemas, e.g., containing conventional suppository bases (e.g., cocoa butter or other glycerides).

In addition to the foregoing dosage forms, the compounds may also be formulated as depot preparations. Such long-acting dosage forms can be administered by implantation or transdermal delivery (e.g., subcutaneous or intramuscular delivery), intramuscular injection, or transdermal patch. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (e.g., acceptable emulsions in oils) or ion exchange resins or the compounds may be formulated as sparingly soluble derivatives, e.g., as a sparingly soluble salt.

The pharmaceutical compositions may also contain suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include calcium carbonate, calcium phosphate, various sugars, starch, cellulose derivatives, gelatin, and polymers such as polyethylene glycol.

Preferred pharmaceutical compositions are those formulated as injectable dosage forms, e.g., intravenous dosage forms, and comprise from about 0.01% to about 100% by weight of the compound of the present invention, based on the total 100% by weight of the pharmaceutical composition. The drug ligand conjugate may be an antibody-cytotoxin conjugate, wherein an antibody is selected that targets a particular cancer.

In some embodiments, the pharmaceutical compositions of the invention further comprise an additional therapeutic agent.

In some embodiments, the additional therapeutic agent is an anti-cancer agent.

In some embodiments, the additional anti-cancer agent is selected from: an antimetabolite, an inhibitor of topoisomerase I and topoisomerase II, an alkylating agent, a microtubule inhibitor, an antiandrogenic agent, a GNRh modulator, or a mixture thereof.

In some embodiments, the additional therapeutic agent is a chemotherapeutic agent.

By "chemotherapeutic agent" herein is meant a chemical compound useful in the treatment of cancer. Examples include, but are not limited to: gemcitabine (Gemcitabine), Irinotecan (Irinotecan), Doxorubicin (Doxorubicin), 5-Fluorouracil (5-Fluorouracil), cytarabine (Cytosine arabinoside, "Ara-C"), Cyclophosphamide (Cyclophosphamide), Thiotepa (Thiotepa), Busulfan (Busulfan), cytotoxins, taxol, Methotrexate (Methotrexate), Cisplatin (Cisplatin), Melphalan (Melphalan), Vinblastine (Vinblastatin), and Carboplatin (Carboplatin).

In some embodiments, the second chemotherapeutic agent is selected from: tamoxifen (tamoxifen), raloxifene (raloxifene), anastrozole (anastrozole), exemestane (exemestane), letrozole (letrozole), imatinib (imatanib), paclitaxel (paclitaxel), cyclophosphamide (cyclophosphamide), lovastatin (lovastatin), mimosine (minosine), gemcitabine (gemcitabine), cytarabine (cyarambine), 5-fluorouracil, methotrexate, docetaxel (docetaxel), goserelin (golelin), vincristine (vincristine), vinblastine (vinblastine), nocodazole (nocodazole), teniposide (teniposide), etoposide (etoposide), gemcitabine, camptothecin (epirubicin), vinorelbine (vinorelbine), reburnine (reburnine), erythromycin (idarubicin), idarubicin (idarubicin), idarubicin, or (idarubicin, ida.

Reagent kit

In another aspect, the invention provides a kit comprising one or more of the compounds or compositions of the invention and instructions for using the compounds or compositions. In an exemplary embodiment, the invention provides a kit for coupling a linker arm of the invention with another molecule. The kit includes a linker and instructions for attaching the linker to a specific functional group. The kit further comprises one or more of a cytotoxic drug, a targeting agent, a detectable label, a pharmaceutically acceptable salt, or a buffer. The kit may also include a container and optionally one or more vials, test tubes, flasks, bottles, or syringes. Other forms of kits will be apparent to those skilled in the art and are within the scope of the invention.

Medical use

In another aspect, the present invention provides a method for treating a disease condition in a subject in need of such treatment, said method comprising administering to said subject a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

In addition to the compositions and constructs described above, the invention also provides various uses of the compounds of the invention. Uses of the compounds of the invention include: killing or inhibiting growth, proliferation or replication of tumor cells or cancer cells, treating cancer, treating precancerous conditions, preventing proliferation of tumor cells or cancer cells, preventing cancer, preventing proliferation of cells expressing autoimmune antibodies. These uses include administering an effective amount of a compound of the invention to an animal, such as a mammal or a human, in need thereof.

The compounds of the invention are useful for treating diseases (e.g., cancer) in animals such as humans. The present invention provides compositions and their use in the treatment of tumors, said use comprising providing to a subject a pharmaceutically effective amount of a composition of the present invention in a pharmaceutically acceptable manner.

"cancer" as used herein refers to a pathological condition in the human body characterized by uncontrolled cellular proliferation. Examples include, but are not limited to: cancer, lymphoma, blastoma, and leukemia. More specific examples of cancer include, but are not limited to: lung (small cell and non-small cell) cancer, breast cancer, prostate cancer, carcinoid cancer, bladder cancer, gastric cancer, pancreatic cancer, liver cancer (hepatocellular carcinoma), hepatoblastoma, colorectal cancer, head and neck cancer, squamous cell carcinoma, esophageal cancer, ovarian cancer, uterine cancer, endometrial cancer, mesothelioma, melanoma, sarcoma, osteosarcoma, liposarcoma, thyroid cancer, desmoid tumor, Acute Myeloid Leukemia (AML), and Chronic Myeloid Leukemia (CML).

As used herein, "inhibit" or "treatment" refers to a decrease, therapeutic, and prophylactic treatment, wherein the object is to decrease or prevent a specified pathological disorder or condition. In one embodiment, a cancer patient may experience a reduction in tumor size following administration of a compound of the invention. "treating" includes (1) inhibiting a disease in a subject suffering from or exhibiting pathology or symptomatology of the disease; (2) alleviating a disease in a subject suffering from or exhibiting pathology or symptomatology of the disease; and/or (3) any measurable reduction in disease affecting a subject or patient suffering from or exhibiting the pathology or symptomatology of the disease. The compounds of the invention may prevent the growth and/or kill cancer cells to the extent that the compounds of the invention are cytostatic and/or cytotoxic.

By "therapeutically effective amount" herein is meant an amount of a compound provided herein that is effective to "treat" a disorder in a subject or mammal. In the case of treating cancer, a therapeutically effective amount of the drug may reduce the number of cancer cells, reduce the size of the tumor, inhibit infiltration of cancer cells into peripheral organs, inhibit tumor metastasis, inhibit tumor growth to a certain extent, and/or alleviate one or more of the symptoms associated with cancer to a certain extent.

Administration "in combination with" one or more other therapeutic agents includes simultaneous administration and sequential administration in any order. The term "pharmaceutical combination" as used herein refers to a product obtained by mixing active ingredients or combining active ingredients, and includes both fixed and non-fixed combinations of active ingredients. The term "fixed combination" means that the active ingredients (e.g. a compound of formula (1)) and the co-agent are administered to a patient simultaneously in a single entity or dose. The term "non-fixed combination" means that the active ingredients (e.g. a compound of formula (1)) and the co-agent are administered to a patient as separate entities either simultaneously or sequentially (without specific time limits) such administration providing a therapeutically effective amount of the active ingredients to the patient. The latter are also useful in combination therapy, e.g., the administration of three or more active ingredients.

In some embodiments, the disease condition is a tumor or cancer. In some embodiments, the cancer or tumor is selected from: gastric, colon, rectal, liver, pancreatic, lung, breast, cervical, uterine corpus, ovarian, testicular, bladder, kidney, brain/central nervous system, head and neck, throat, hodgkin's disease, non-hodgkin's lymphoma, multiple myeloma, leukemia, melanoma, non-melanoma skin cancer, acute lymphocytic leukemia, acute myelogenous leukemia, ewing's sarcoma, small cell lung cancer, chorioepithelioma, rhabdomyosarcoma, Wilms tumor, neuroblastoma, hairy cell leukemia, oropharyngeal, esophageal, laryngeal, or lymphoma.

In some embodiments, the disease condition comprises abnormal cell proliferation, such as a precancerous lesion.

The invention is particularly useful for treating cancer in an animal and inhibiting the proliferation of tumor cells or the proliferation of cancer cells in an animal. Cancer or a precancerous condition, including a tumor, metastasis, or any disease or disorder characterized by uncontrolled cell growth, may be treated or prevented by administering a drug-ligand complex of the present invention. The compounds deliver an activating moiety to a tumor cell or cancer cell. In some embodiments, the targeting moiety specifically binds to a cancer cell or tumor cell-associated antigen. Since the activating moiety is close to the ligand, upon internalization, the activating moiety can be taken up into the interior of the tumor or cancer cell, for example, by receptor-mediated endocytosis. The antigen may be attached to a tumor cell or a cancer cell or may be an extracellular matrix protein associated with a tumor cell or a cancer cell. Once inside the cell, the linker is hydrolytically cleaved or enzymatically cleaved by a tumor cell-associated protease or a cancer cell-associated protease, thereby releasing the activated moiety. The released activated moieties are then free to diffuse and induce or enhance the immune activity of immune or tumor cells. In alternative embodiments, the activated moiety is cleaved from the compound tumor microenvironment, followed by drug infiltration into the cells.

Representative examples of precancerous conditions that may be targeted by the compounds of the present invention include: metaplasia, hyperplasia, abnormalities, colorectal polyps, actinic keratosis, actinic cheilitis, human papilloma virus, leukoplakia, lichen planus, and bowen's disease.

Representative examples of cancers or tumors that may be targeted by the compounds of the invention include: lung cancer, colon cancer, prostate cancer, lymphoma, melanoma, breast cancer, ovarian cancer, testicular cancer, central nervous system cancer, kidney cancer, pancreatic cancer, stomach cancer, oral cancer, nasal cancer, cervical cancer, and leukemia. It will be apparent to one of ordinary skill in the art that the particular targeting moiety used in the compound that targets the activating moiety to the tumor tissue to be treated with the drug can be selected (i.e., a targeting agent specific for a tumor-specific antigen is selected). Examples of such targeting moieties are known in the art and include anti-Her 2 for the treatment of breast cancer, anti-CD 20 for the treatment of lymphoma, anti-PSMA for the treatment of prostate cancer and anti-CD30 for the treatment of lymphoma, including non-hodgkin's lymphoma.

In some embodiments, the abnormally proliferating cell is a cancer cell.

In some embodiments, the cancer is selected from: breast cancer, colorectal cancer, diffuse large B-cell lymphoma, endometrial cancer, follicular lymphoma, gastric cancer, glioblastoma, head and neck cancer, hepatocellular cancer, lung cancer, melanoma, multiple myeloma, ovarian cancer, pancreatic cancer, prostate cancer, and renal cell carcinoma.

In some embodiments, the invention provides compounds for killing cells. The compound is administered to the cells in an amount sufficient to kill the cells. In exemplary embodiments, the compound is administered to a subject with the cells. In another exemplary embodiment, the administration delays or stops the growth of a tumor comprising the cell (e.g., the cell can be a tumor cell). For administration with delayed growth, the growth rate of the cells should be at least 10% less than the growth rate prior to administration. Preferably, the growth rate is delayed by at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or stopped completely.

Furthermore, the present invention provides a compound or a pharmaceutical composition of the present invention for use as a medicament. The invention also provides compounds or pharmaceutical compositions for killing, inhibiting or delaying proliferation of tumor or cancer cells, or for treating diseases involving TLR7 and/or TLR 8.

Effective dose

Pharmaceutical compositions suitable for use in the present invention include compositions comprising a therapeutically effective amount of the active ingredient, i.e., an amount effective to achieve the desired purpose. The actual amount effective for a particular application will depend, inter alia, on the condition being treated. Determination of an effective amount is well within the ability of those skilled in the art (especially in light of the specific disclosure herein).

For any of the compounds described herein, a therapeutically effective amount can be determined by cell culture assays. The target blood concentration is the concentration of the active compound that is capable of inhibiting cell growth or division. In a preferred embodiment, at least 25% of the cellular activity is inhibited. Target blood concentrations of active compounds capable of inducing inhibition of cellular activity of at least about 30%, 50%, 75% or even 90% or more are presently preferred. The percentage of inhibition of cellular activity in the patient can be monitored to assess the appropriateness of the achieved blood concentration, and the dosage can be adjusted up or down to achieve the desired percentage of inhibition.

A therapeutically effective amount for use in humans can also be determined by animal models, as is known in the art. For example, a dose for use in humans may be formulated to achieve an effective circulating concentration that has been found in animals. As described above, the dose in humans can be adjusted by monitoring cytostatic and up-or down-regulating the dose.

Therapeutically effective doses can also be determined by human data for known compounds exhibiting similar pharmacological activity. The dosage used may be adjusted based on the relative bioavailability and potency of the administered compound compared to known compounds.

It is well within the ability of one of ordinary skill in the art to adjust the dosage to achieve maximum efficacy in the human body based on the methods described above and other methods well known in the art.

In the case of topical administration, the systemic circulating concentration of the administered compound is not particularly important. In such cases, the compound is administered to achieve a concentration effective to achieve the desired result in the local area.

For use in the prevention and/or treatment of diseases associated with abnormal cell proliferation, it is preferred that the compound be administered at a circulating concentration of about 0.001 μ M to 20 μ M, preferably about 0.01 μ M to 5 μ M.

Patient doses for oral administration of the compounds described herein are typically from about 1 mg/day to about 10,000 mg/day, more typically from about 10 mg/day to about 1,000 mg/day, and most typically from about 50 mg/day to about 500 mg/day. Typical dosages will be in the range of about 0.01 mg/kg/day to about 150 mg/kg/day, more typically in the range of about 0.1 mg/kg/day to about 15 mg/kg/day, most typically in the range of about 1 mg/kg/day to about 10 mg/kg/day, e.g., 5 mg/kg/day or 3 mg/kg/day, based on the weight of the patient.

In at least some embodiments, the patient dose to delay or inhibit tumor growth can be 1 μmol/kg/day or less. For example, the patient dose may be 0.9. mu. mol/kg/day, 0.6. mu. mol/kg/day, 0.5. mu. mol/kg/day, 0.45. mu. mol/kg/day, 0.3. mu. mol/kg/day, 0.2. mu. mol/kg/day, 0.15. mu. mol/kg/day, or 0.1. mu. mol/kg/day or less (with reference to moles of drug). Preferably, the antibody drug conjugate delays tumor growth when administered at a daily dose for a period of at least five days.

For other modes of administration, the dosage and interval may be adjusted separately to provide blood levels of the administered compound that are effective for the particular clinical indication being treated. For example, in one embodiment, a compound according to the present invention may be administered multiple times per day at relatively high concentrations. Alternatively, it may be more desirable to administer the compounds of the present invention at a minimum effective concentration and to use a less frequent dosing regimen. This would provide a treatment regimen commensurate with the severity of the disease in the individual.

Using the teachings herein, an effective treatment regimen can be scheduled without causing significant toxicity, and yet be completely effective in treating the clinical symptoms exhibited by a particular patient. Such a schedule should include careful selection of the active compound by considering various factors such as the potency of the compound, the relative bioavailability, the body weight of the patient, the presence and severity of adverse side effects, the preferred mode of administration and the toxicity profile of the agent selected.

While preferred embodiments of the present invention are described and shown herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, modifications, and substitutions will occur to those skilled in the art without departing from the invention. It should be understood that alternative embodiments of the invention described herein may be used to practice the invention. The scope of the invention is defined by the appended claims, and methods and structures within the scope of these claims and their equivalents are also intended to be covered by the appended claims.

Examples

The invention is further illustrated, but not limited, by the following examples, which illustrate methods of preparing the compounds of the invention.

Example 1

Generation of her2 or egfr transfected L cell lines

Reagent: l cells from ATCC (Manassas, VA; Cat No. CRL2648), Her2/pEZ-Lv105 or egfr/pCMV cDNA, glucose DMEM, L-glutamine, Lipofectamine2000(Invitrogen; Carlsbad, Calif.) from Sino Biological Inc. (Cat No. H10004) or GeneCopoeia (Cat No. Z2866).

To prepare cell lines for screening of conjugated trastuzumab, L cells expressing labeled Her2 or EGFR were generated. Her2/pEZ-Lv105 or egfr/pCMV cDNA constructs were transfected into L cells (the cells were grown in high concentration glucose DMEM +10% FBS +2mM L-glutamine) by standard Lipofectamine2000 protocols.

Binding assays (FACS analysis)

To determine the binding capacity of conjugated trastuzumab, FACS analysis was performed on L cells expressing human her 2. Briefly, 100. mu.l of about 10⁶L cells with transiently transfected her2 were incubated with varying amounts of trastuzumab-conjugated antibody, using PBS or secondary antibody alone or irrelevant huIgG as negative controls. After washing, cells were resuspended in FACS buffer and incubated with 20 μ Ι _ of mouse anti-human IgG coupled to phycoerythrin (Mu anti-human PE) secondary antibody for 30 min at room temperature in a 100 μ Ι reaction volume. After washing, cells were fixed in 200 μ L of 2% paraformaldehyde/PBS followed by flow cytometry analysis. The same procedure was used for irrelevant human IgG antibodies as isotype controls to set the baseline PMT/cell line. In the BDFlow cytometry analysis was performed on and the geometric mean fluorescence intensity of each sample was recorded. The recorded data was analyzed using FlowJo software. The results are shown in FIG. 1.

Generation of antibody toll-like receptor ligand conjugates

Reagent: trastuzumab (Roche/Genentech Corporation, South San Francisco, Calif.), cetuximab (Merck), MC-val-cit-PAB (MC-vc-PAB-TLRL) linked to resiquimod or MC- (MC-TLRL) linked to resiquimod (synthesized in Contract Research Organization, China), sodium borate, sodium chloride, Dithiothreitol (DTT), Sephadex G25, DTPA, DTNB, and maleimidocaproyl-monomethyl (Sigma-Aldrich, Milwaukee, Wisconsin).

Drugs used to generate antibody TLR ligand conjugates include trastuzumab and resiquimod (TLRL), in some cases cetuximab is used for the conjugate. The linker used to generate the TLAC is a cleavable linker MC-vc-PAB or a non-cleavable linker MC.

Preparation of trastuzumab MC-TLRL or cetuximab MC-TLRL

Trastuzumab was removed by buffer exchange at a concentration of 20mg/mLWas purified, and the antibody dissolved in 500mM sodium borate and 500mM sodium chloride at pH 8.0 was treated with an excess of 100mM Dithiothreitol (DTT). After incubation at 37 ℃ for about 30 minutes, elution was carried out by passing through the elution exchange buffer on Sephadex G25 resin and with PBS containing 1mM DTPA. The thiol/Ab value was determined by measuring the concentration of reduced antibody detected by absorbance of the solution at 280nm and the thiol concentration determined by reaction with DTNB (Sigma-Aldrich, Milwaukee, Wisconsin) and absorbance at 412 nm. The reduced antibody dissolved in PBS was frozen on ice. The drug linker reagent (Mc-linked to resiquimod) dissolved in DMSO was diluted in acetonitrile and water at known concentrations and added to the frozen reducing antibody in PBS. After about 1 hour, the reaction was quenched by addition of excess maleimide and any unreacted antibody thiol groups were masked. In some cases, coupling to lysine of an immunoglobulin is performed by standard procedures. The reaction mixture was concentrated by centrifugal ultrafiltration, and the conjugated antibody was purified and desalted by elution from G25 resin in PBS, filtered through a 0.2 μm filter under sterile conditions, and stored frozen. Cetuximab MC-TLRL was prepared using the same procedure.

Of trastuzumab MC-vc-TLRL or cetuximab MC-vc-TLRLPreparation of

The antibody was linked to the TLRL via cysteine from maleimidocaproyl-valine-citrulline (vc) -p-aminobenzyloxycarbonyl (MC-vc-PAB). The MC-vc-PAB linker is cleavable by an intercellular protease such as cathepsin B, which when cleaved releases the free drug (Doronina et al, nat. Biotechnol.,21: 778-. Purified trastuzumab was dissolved in 500mM sodium borate and 500mM sodium chloride at pH 8.0 and further treated with an excess of 100mM Dithiothreitol (DTT). After incubation at 37 ℃ for about 30 minutes, the eluate was passed through the elution exchange buffer on Sephadex G25 resin and eluted with PBS containing 1mM DTPA. thiol/Ab values were determined by measuring the concentration of reduced antibody detected by absorbance of the solution at 280nm and the thiol concentration detected by reaction with DTNB (Sigma-Aldrich, Milwaukee, Wisconsin) and absorbance at 412nm (extinction coefficient =13600 cm)^-1M^-1) To detect. The reduced antibody dissolved in PBS was frozen on ice. The resiquimod-linked MC-val-cit-PAB-PNP in DMSO was dissolved in acetonitrile and water and added to the frozen reduced antibody in PBS. After 1 hour incubation, the reaction was quenched by addition of excess maleimide and any unreacted antibody thiol groups were masked. In some cases, coupling to lysine of an immunoglobulin is performed by standard procedures. The reaction mixture was concentrated by centrifugal ultrafiltration, and the antibody-drug conjugate was purified and desalted by elution from G25 resin in PBS, filtered through a 0.2 μm filter under aseptic conditions, and stored frozen. Cetuximab MC-vc-TLRL was prepared using the same procedure.

Typically, conjugation reactions of antibodies to MC-TLRL or MC-vc-TLRL produce heterogeneous mixtures comprising conjugated antibodies linked to varying amounts of the TLRL drug, i.e., drug partitioning from 1 to about 8 drug loads. Thus, antibody MC-TLRL or antibody MC-vc-TLRL includes a mixture of isolated purified species molecules and average drug loadings ranging from 1 to 8. By controlling the process, drug loading was 3 to 5. The average number of TLRL drug moieties per antibody in a compound preparation obtained by a conjugation reaction can be characterized by conventional means, such as mass spectrometry, ELISA analysis, electrophoretic separation and HPLC. The quantitative distribution of the antibody MC-TLRL or antibody MC-vc-TLRL according to the drug can also be determined. The mean number of drug payloads in a particular preparation of antibody conjugated to TLRL can be determined by ELISA (Hamblett et al (2004) Clinical Cancer Res.10: 7063-. However, the partition value of the drug cannot be distinguished by antibody-antigen binding and ELISA detection limits. Furthermore, ELISA assays for detecting antibody drug conjugates cannot determine where the drug moiety is attached to the antibody, e.g., heavy or light chain fragments or specific amino acid residues. In some cases, isolation, purification, and characterization of homogeneous trastuzumab MC-TLRL or trastuzumab MC-vc-TLRL (where the drug in trastuzumab MC-TLRL or trastuzumab MC-vc-TLRL with other drug loading is a particular value) may be achieved by means such as reverse phase HPLC or electrophoretic separation.

Antibody-dependent cell-mediated cytotoxicity Assay (ADCC)

Reagent: SKBR3 cells (ATCC, Cat No. HTB-30), McCoy's 5A (Invitrogen, Cat No.22400, Lot No. 747809), RPMI-1640 (Invitrogen, Cat No.11835, Lot No. 764956), FCS (Hyclone, Cat No. SH30084.03, Lot No. GRH0054), Ficoll-Hypaque (Amersham Biosciences, Piscataway, NJ; Cat No. 17-1440-02), 96-well plates (Costar, Cat No.3599, Cat No. 3916), Trypan blue (Invitrogen Cat 152No 50-061), LDH kit (Promega, Cat No. G7891), ELISA reader 5 (Molece device). Humanized antibodies(Genentech Corporation, South San Francisco, Calif.; trade name trastuzumab) was reconstituted in water to make a10 mg/ml stock solution prior to use.

To determineWhether trastuzumab coupled with high efficiency loading TLRL still has effector function, several different coupled forms of antibody-dependent cell-mediated cytotoxicity (ADCC) activity were tested and compared to trastuzumab reference material that has been shown to have significantly improved ADCC activity. ADCC assays were performed using Peripheral Blood Mononuclear Cells (PBMCs) from healthy donors as effector cells and using the human SKBR3 cell line as target cells. The first day, 1X 10⁴The cells were seeded at 100. mu.L of SKBR3 in 96-well plates and incubated at 37 ℃ for 48 hours in 5% CO 2.

On the third day, fresh human PBMCs were prepared from human blood obtained from healthy volunteer donors. Human venous blood samples were collected in ammonium citrate (ACD-A) tubes. The tube was inverted several times and the whole blood was transferred to a 50ml conical tube. Blood was diluted 1:3 with PBS in 2% FBS. Ficoll-Hypaque was slowly dispensed to the bottom of the blood/PBS mixture. The samples were centrifuged at 2400rpm for 30 minutes at room temperature, followed by removal of the upper layer (plasma/PBS) by aspiration. The blood sediment layers (Buffy coat) were collected with a sterile pipette and pooled in a 50ml conical tube. If a WBC clot is still visible under the buffy coat, then all WBC material is collected, taking care not to remove excess Ficoll-Hypaque. Sterile PBS-2% FBS was added and mixed by inversion. The diluted PBMC suspension was centrifuged at 250 × g for 20 minutes at room temperature to collect the cell pellets. The PBMC pellets were suspended in RPMI-1640 medium with 2% heat-inactivated FBS, washed and checked for viable cell number by trypan blue exclusion. Preparation 1.2X 10⁷cell/mL density is ready for use. Meanwhile, the final concentration of the antibody prepared in RPMI-1640 was from 12. mu.g/mL, 4. mu.g/mL, 1.2. mu.g/mL, 0.4. mu.g/mL, 0.12. mu.g/mL, 0.04. mu.g/mL, 0.012. mu.g/mL, 0.004. mu.g/mL to 0. mu.g/mL.

A series of dilutions of test and control antibodies were then added to the wells containing target cells, 50 μ L of PBMC effector cells in RPMI-1640 medium was added to each well (effector to target ratio of 60: 1), and incubated for an additional 17 hours. At the end of the incubation, the plates were centrifuged and the supernatants were analyzed for Lactate Dehydrogenase (LDH) activity using an LDH measurement kit. Cell lysis was quantified by absorbance at 490nm using a microplate reader. The absorbance of wells containing only target cells served as background controls, while wells containing target cells lysed with Triton-X100 provided the largest available signal. Antibody-independent cytotoxicity was measured in wells containing target and effector cells without addition of antibody. Cytotoxicity was calculated according to the following equation:

% cytotoxicity =100% x [ a490nm (sample) -a 490 (target cell) -a 490 (effector cell ]/[ a490nm (lysed target cell) -a 490nm (target cell) ]

Mean ADCC values in duplicate sample dilutions were plotted against antibody concentration and EC50 values and ADCC (%) maximization were obtained by fitting to Prism5 (GraphPad).

Enrichment of human Dendritic Cells (DCs) from PBMCs

Human PBMCs were prepared from blood sediments obtained from healthy volunteer donors by Ficoll centrifugation. Dendritic cells were enriched by magnetic beads (Miltenyi Biotec) with a mixture of anti-CD3, anti-CD 19, anti-CD 20, anti-CD 14 and anti-CD 16 antibodies from human PBMC by using negative subtraction. Enriched DCs were stained with goat anti-mouse FITC (lineage), HLA-DR-APCCy7, CD123-BV421 and CD 11C-APC. Stained cells were analyzed on BD LSR Fortessa. anti-CD3 monoclonal antibody, anti-CD 4 monoclonal antibody, anti-CD 11C monoclonal antibody, anti-CD 19 monoclonal antibody, anti-CD 14 monoclonal antibody, anti-CD 16 monoclonal antibody, anti-CD 123 monoclonal antibody were purchased from BD Biosciences or Biolgend.

FIG. 3A shows the percentage of DCs before and after enrichment. The numbers in the upper two panels represent the percentage of DC (HLA-DR + Lin-) of total cells before and after lineage depletion. The numbers in the lower panel represent the percentage of mdcs (CD 11C + CD 123-) and pdcs (CD 123+ CD 11C-) of total DCs before and after lineage depletion.

Stimulation of enriched human DCs and cytokine expression

Mixing 1-2X 10⁵The enriched DCs were seeded in 96-well plates in 100. mu.l of medium, 100. mu.l of diluted stimulating agent was added to the plates and incubated in an incubator at 37 ℃ for 20 to 22 hours. Supernatants were collected and analyzed by ELISA (Mabtech AB) for human IFN-. alpha.IL-6, IL-12(p70) and TNF-. alpha..

Statistical analysis

Significance for all comparisons was calculated using the Student two-tailed t-test, assuming that the variance between the mock and sample groups was not equal, the results were considered significant when p < 0.05. Correlations between parameters were evaluated using the spearman rank correlation test, P values <0.05 were considered statistically significant.

In vivo tumor cell killing assays using trastuzumab TLRL conjugates or cetuximab TLRL conjugates

For the study of patient-derived gastric cancer xenograft (PDX) mouse models, 6 to 8 week-old female Balb/c nude mice (obtained from SLAC, Shanghai, China) were used for tumor fragment transplantation. Animals were kept on normal nude mouse diets and maintained in SPF Animal facilities according to the laboratory Animal Care and Use guidelines and protocols of the Institutional Animal Care and Use Committee. Will measure about 15mm³To 30mm³Patient(s) of (1) STO #69 gastric tumor fragments were transplanted (subcutaneously) to the right side of Balb/c nude mice.

For the study of lung cancer xenograft models, 6 to 8 week old female Balb/c nude mice were used for tumor fragment transplantation. Human lung cancer cell line H1650 (ATCC, Cat. # CRL 5883) was cultured in RPMI-1640 medium containing 10% serum and transplanted (subcutaneously) to the right side of Balb/c nude mice. Each mouse received 2X 10 of 100. mu.l of basement membrane per inoculation⁶And (4) cells.

Administration by intravenous route of a drug containing 5mg/kg to 20mg/kg antibody or a drug containing 20mg/kg Tarceva (Tarceva) or a reference drug (QWKx 3)A medicine is provided. Tumors were measured once a week by calipers to determine their subcutaneous growth. Two-dimensional dimensions of the tumors were measured twice weekly with calipers. Tumor volume was calculated using the following equation: tumor volume = (length × width 2) × 0.5. Mean tumor volume or body weight was plotted using the plotting program Prism5 (GraphPad). The endpoints of the efficacy study were set at 30 to 45 days after the first treatment or when tumor size reached 2000mm³When this happens (whichever comes first). If the mice lost more than 20% of their body weight or were ill too heavily and were unable to get enough food or water, they were removed from the study and euthanized. Tumors were collected from mice at the endpoint, in LN₂Half frozen and half fixed in formalin for preparation of FFPE tissue.

Claims

1. A compound having the structure of formula (Ib) or a pharmaceutically acceptable salt or solvate thereof:

TM-L-AM （Ib），

wherein the dotted line representsIn the presence or absence of a chemical bond,is the point to be connected to the connector;

x is S or-NR₁，R₁is-W₀—W₁—W₂—W₃—W₄；

W₀Is a bond, alkyl, alkenyl, alkynyl, alkoxy or-alkyl-S-alkyl- -,

W₂is a chemical bond, -O-, -C (O) -, -C (S) -or-S (O)₂—，

W₄comprises the following steps: hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, aryl, aryloxy, heteroaryl or heterocyclyl, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, - - -NH₂Nitro, -alkyl-hydroxy, -alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，--C(O)-O-R₄，--S-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，--NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN, - -NO₂and-SH, wherein R₄Independently are: hydrogen, alkyl, alkenyl, - -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl;

z is: hydrogen, alkyl, alkenyl, alkynyl, alkoxy, aryl, haloalkyl, heteroaryl, heterocyclylEach of which may be optionally substituted with one or more substituents selected from: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclyl, halo, cyano, nitro, - - -N (R)₅)₂- - (O) -alkyl, - -alkoxy-alkenyl, - -C (O) -alkyl, - -C (O) -O-alkyl, - -O-C (O) -alkyl, - -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl and- -CO-heteroaryl, where R is₅Independently from each other: hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkylheteroaryl;

r is: hydrogen, alkyl, alkoxy, haloalkyl, halogen, aryl, heteroaryl, heterocyclyl, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, - - -NH₂Nitro, -alkyl-hydroxy, -alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄，--C(O)-NH-R₄，--C(O)-NR₄R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，--C(O)-O-R₄，--O-C(O)-R₄，--S-R₄，--C(O)-S-R₄，--S-C(O)-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，--NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN and- -SH, in which R₄Independently are: hydrogen, alkyl, alkenyl, alkoxy, - -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl, or haloalkyl;

n is 0,1, 2,3 or 4;

y is-NR₆R₇，–CR₆R₇R₈or-alkyl-NH₂Each of which may be optionally substituted with one or more substituents selected from: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, - -NH₂The presence of, halogen,--N(R₅)₂-alkoxy-alkyl, -alkoxy-alkenyl, -C (O) -alkyl, -C (O) -O-alkyl, -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl and- -CO-heteroaryl,

wherein R is₆，R₇And R₈Independently are: hydrogen, alkyl, alkenyl, alkoxy, alkylamino, dialkylamino, alkylthio, arylthio, - -alkyl-hydroxy, - -alkyl-C (O) -O-R₉- - (O) -R- -alkyl-C₉or-alkyl-O-C (O) -R₉Wherein R is₅Each independently is hydrogen, alkyl, haloalkyl, - -alkyl-aryl or-alkyl-heteroaryl, wherein R₉Is hydrogen, alkyl, alkenyl, halogen or haloalkyl;

optionally, X and Z together form a 5 to 9 membered ring.

2. The compound of claim 1, wherein L is represented by the structure of the following general formula (II):

wherein i is each independently 0 or 1;

j is each independently 0,1, 2,3, 4,5 or 6;

each B is independently alkyl, alkenyl, -O-alkyl, -alkyl-O, -S-alkyl, -alkyl-S, -aryl, heteroaryl, heterocyclyl or peptide, each of which is optionally substituted with one or more substituents selected from the group consisting of: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkaneThe group-alkyl-aryl, -alkyl-heteroaryl, -alkyl-heterocyclyl, -O-R₄- - -O-alkyl-R₄，--C(O)-R₄，--C(O)-O-R₄，--S-R₄，--S(O)₂-R₄，--NHR₄- - -NH-alkyl-R₄Halogen, - -CN, - -NO₂and-SH, wherein R₄Comprises the following steps: alkyl, alkenyl, - -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl or haloalkyl.

3. The compound of claim 1, wherein X is-NR₁，R₁Comprises the following steps: alkyl, -alkyl-W₄- -alkyl-O- -W₄- -alkyl-NH- -C (O) - -W₄- -alkoxy- -NH- -C (O) - -W₄- -alkyl-NH- -C (O) - -NH- -W₄- -alkoxy- -NH- -C (O) - -NH- -W₄- -alkyl-S (O)₂-W₄or-alkyl-NH-C (S) -W₄。

4. The compound of claim 1, wherein X is S.

5. The compound of any one of claims 1 to 4, wherein Z is hydrogen, alkyl, alkoxy, aryl, heteroaryl, haloalkyl, each of which is optionally substituted with one to three substituents selected from the group consisting of: hydroxy, alkyl, aryl, heteroaryl, heterocyclyl, cyano, - -alkoxy-alkyl, nitro and- -N (R)₅)₂Wherein R is₅Independently from each other: hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkylheteroaryl.

6. The compound of any one of claims 1 to 5, wherein n is 1 or2, R is aryl or heteroaryl, each of which is optionally substituted with one to three substituents selected from: hydroxy, alkoxy, -alkyl-hydroxy, -O-R₄- - -O-alkyl-R₄- - (O- -R) alkyl-O- -R₄，--C(O)-R₄，--C(O)-NH-R₄，--C(O)-NR₄R₄- - (O) -R- -alkyl-C₄- - (O) -O- -R₄，--C(O)-O-R₄，--O-C(O)-R₄，--S-R₄，--C(O)-S-R₄，--S-C(O)-R₄，--S(O)₂-R₄，--NH-S(O)₂-R₄- - (Y- -O) - -alkyl-S-R₄- - -alkyl-S (O)₂-R₄，--NHR₄，--NR₄R₄- - -NH-alkyl-R₄Halogen, - -CN and- -SH, in which R₄Independently hydrogen, alkyl, alkenyl, alkoxy, -alkyl-hydroxy, aryl, heteroaryl, heterocyclyl, or haloalkyl.

7. The compound of any one of claims 1 to 6, wherein Y is-NH₂- - (O- -NH) - -alkyl-NH₂Each of which is optionally substituted with one to three substituents selected from the group consisting of: alkyl, alkoxy, alkenyl, and alkynyl.

8. The compound of any one of claims 2 to 7, wherein D in the linker moiety is selected from the following structures of general formula (V) to general formula (VII):

A. b, i and j are as defined above.

9. The compound of any one of claims 1 to 8, wherein the TM comprises an immunoglobulin, a protein, a small molecule, a nanoparticle, or a nucleic acid.

10. The compound of claim 9, wherein the TM specifically binds to a tumor cell or preferentially binds to a tumor cell relative to a non-tumor cell.

11. The compound of claim 10, wherein the TM specifically binds to a tumor antigen on the tumor cell.

12. The compound of claim 11, wherein the tumor antigen is selected from the group consisting of: CD2, CD19, CD20, CD22, CD27, CD33, CD37, CD38, CD40, CD44, CD47, CD52, CD56, CD70, CD79, CD137,4-1BB,5T4, AGS-5, AGS-16, angiopoietin 2, B7.1, B7.2, B7 4, B7H 4, B7H 4, B7H 4, BT-062, BTLA, CAIX, carcinoembryonic antigen, CTLA4, Cripto, ED-B, ErbB4, ErbB4, ErbB4, EGFL 4, EphA 4, EphB 4, FAP, fibronectin, folate receptor, ganglioside 4, GM 4, glucocorticoid receptor, tumor necrosis factor receptor induced by TNF receptor (PGCA), PGCA-100, PGCA-IgG-1, PGA-4, PGCA-1, PGA-4, PGCA-C, PGA, PGX, PGA-4, PGX, PGC-4, PGA-4, PGX, PGC-4, PGX, PGS-4, PGX, PGS-3, PGX, PGS-7, PGS-3, PGS-7, PGS-4, PGS-X, PGA-4, PGA-X, PGS-7, PG, TAG-72, tenascin, TIM3, TRAILR1, TRAILR2, VEGFR-1, VEGFR-2, VEGFR-3, and variants thereof.

13. The compound of claim 9, wherein the TM comprises an antibody or functional fragment thereof.

14. The compound of claim 9, wherein TM comprises: rituximab, herceptin (trastuzumab), erbitux (cetuximab), victoria (panitumumab), arzerra (ofatumumab), Benlysta (belimumab), Yervoy (ipilimumab), Perjeta (pertuzumab), Tremelimumab, Nivolumab, Dacetuzumab, Urelumab, MPDL3280A, Lambrolizumab, blinatumumab, aldesleukin (aldesleukin), aemtuzumab, arivulvonic acid (alitetinin), allopurinol, altretamine (altretamine), amifostine (amifosiltine), anastrozole (anastrozole), arsenic, asparaginase, liase, liotin, betadine capsule (beaxarotene), saxagliptin (blevacizine), sulbactam (blevaccaritin), bletacrine (bletacrine), bletacrine (bletacrine, blevaccarinicarbane), Blevaccarinicarbane (BCG), blevaccarinicarbane, blevaccarinicarbate, blevacarbine, bleomycin, blevacarbine, blevaccarinicarbate, bleomycin, blevacarbine, intravenous drip, Cytarabine liposome, dacarbazine (dacarbazine), dactinomycin, alfa bepotin (dabepoetin alfa), daunorubicin hydrochloride liposome, daunorubicin hydrochloride, daunorubicin, dnitukin diftitox, dexrazoxane (dexrazoxane), docetaxel, doxorubicin liposome, dromostone propionate (domostanolone propinate), eliott's B solution, epirubicin, eoetin alfa estramustine, etoposide phosphate, Poposide (VP-16), exemestane, flastim, fluorouracil (intra-arterial use), fludarabine (fludarabine), fluorouracil (5-FU), fulvestrant (fulvestrant), gemtuzumab geuzumab, sertraline, hydroxyurea acetate, ibrutinib (ibrutin), interferon alfa 2, and isosfamide, Irinotecan, troconazole, calcium folinate, levamisole (levamisole), lomustine (CCNU), dichloromethyldiethylamine (nitrogen mustard), megestrol acetate, melphalan (L-PAM), mercaptopurine (6-MP), mesna, methotrexate, methoxsalen, mitomycin C, mitotane, mitoxantrone, phenthoquinone, nandrolone propionate, nfetumomab, LOddC, orelevin, oxaliplatin, paclitaxel, pamidronate, pegamunase, pemetrexed, pegavanase, pegavastatin, Pegfilgrastim, pentostatin, pipobroman, plicamycin, mithramycin, porphine, procarbazine, quinacrine, labirisin, tigecycline, tenipostine, tenipodine, tenuacil, tenuadine, tavidine, tazidine, teosine, timothricin, 6-D, teosine, tetrodotoxin, D, thiotepa, topotecan, toremifene (toremifene), Tositumomab (Tositumomab), tretinoin (ATRA), uramustine (Uracil Mustard), valrubicin, pantocitabine (valtocitabine, monovLDC), vinblastine, vinorelbine, zoledronic acid (zoledronate), and mixtures thereof.

15. A pharmaceutical composition comprising an effective amount of a compound of any one of claims 1 to 14, and one or more pharmaceutically acceptable carriers.

16. The pharmaceutical composition of claim 15, further comprising an effective amount of an additional therapeutic agent.

17. The pharmaceutical composition of claim 16, wherein the additional therapeutic agent is an anti-cancer agent.

18. The pharmaceutical composition of claim 16, wherein the anti-cancer agent is an anti-metabolite, an inhibitor of topoisomerase I and topoisomerase II, an alkylating agent, a microtubule inhibitor, an anti-androgenic agent, a GNRh modulator, or a mixture thereof.

19. A method of inhibiting proliferation of a tumor cell, the method comprising administering to the tumor cell a compound of any one of claims 1 to 14.

20. A method of treating a disease in a subject, comprising administering to the subject a compound of any one of claims 1 to 14.

21. The method of claim 20, wherein the disease is cancer selected from the group consisting of: gastric, colon, rectal, liver, pancreatic, lung, breast, cervical, uterine corpus, ovarian, testicular, bladder, kidney, brain/central nervous system, head and neck, throat, hodgkin's disease, non-hodgkin's lymphoma, multiple myeloma, leukemia, melanoma, non-melanoma skin cancer, acute lymphocytic leukemia, acute myelogenous leukemia, ewing's sarcoma, small cell lung cancer, chorioepithelioma, rhabdomyosarcoma, Wilms tumor, neuroblastoma, hairy cell leukemia, oropharyngeal, esophageal, laryngeal, or lymphoma.

22. Use of a compound according to any one of claims 1 to 14 in the manufacture of a medicament for treating a disease in a subject.

23. Use of a pharmaceutical composition according to any one of claims 15 to 18 in the manufacture of a medicament for treating a disease in a subject.

24. A compound having the structure of formula (Ib) or a pharmaceutically acceptable salt or solvate thereof:

TM-L-AM (Ib)

wherein,is the point to be connected to the connector;

R₁₀and R₁₁Independently hydrogen, alkyl, alkenyl, aryl, haloalkyl, heteroaryl, heterocyclyl or cycloalkyl, each of which is optionally substituted with one or more substituents selected from: hydroxy, alkoxy, alkyl, alkenyl, alkynyl, halogen, - -N (R)₅)₂-alkoxy-alkyl, -alkoxy-alkenyl, -C (O) -alkyl, -C (O) -O-alkyl, -C (O) -N (R)₅)₂Aryl, heteroaryl, - -CO-aryl, and- -CO-heteroaryl, wherein R is₅Each independently hydrogen, alkyl, haloalkyl, -alkyl-aryl or-alkyl-heteroaryl.

25. A pharmaceutical composition comprising an effective amount of a compound of claim 24, and one or more pharmaceutically acceptable carriers.

26. The pharmaceutical composition of claim 25, further comprising an effective amount of an additional therapeutic agent.