CN104472500B - Composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent - Google Patents

Abstract

The invention discloses a kind of composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent, relate to technical field of pesticide.The composition pesticide of the present invention is made up of Aminothiocarbonylbenzene and triazole bactericidal agent; described Aminothiocarbonylbenzene is the bromo-N-of 3-[4-chloro-2-methyl-6-(methylamino thioformyl) benzene]-1-(3-chloropyridine-2-base)-1H-pyrazoles-5-Methanamide; described triazole bactericidal agent is any one in hexaconazole, Flutriafol, Tebuconazole, Difenoconazole, epoxiconazole, and the mass ratio of described Aminothiocarbonylbenzene and triazole bactericidal agent is 100:1 ~ 1:100.This composition pesticide and pesticidal preparations thereof have obvious potentiation relative to single dose, can effectively preventing diseases and pests of agronomic crop, reduce the using dosage of pesticide, reduce cost accounting, while improving bactericidal and insecticidal activity, also delay the pest and disease damage Drug resistance to composition pesticide.

Description

Composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent

Technical field

The present invention relates to technical field of pesticide, especially relate to the composition pesticide of insecticide and fungicide compounding, be specifically related to a kind of composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent.

Background technology

In recent years, along with the expansion gradually of the development of scale of agricultural production and Greenhouse area, continuous cropping and continuous cropping cropping pattern are more and more general.Crops also there occurs disease while there is insect pest, but at present when preventing and treating these pest and disease damages, peasant household is by insecticide and antibacterial at will existing mixed existing use, does not only reach potentiation, easily plays antagonism on the contrary, and drug effect declines.This unscientific medication, causes that again medicament using dosage increases, causes the waste of medicament, the pesticide residues on crops exceed standard, and are unfavorable for the protection of environment.For this reason, it may be necessary to develop more compound synergic, can the combination medicament that can sterilize again of parasite killing.

Chinese invention patent CN103109816A discloses thiobenzoyl aminated compounds and application thereof, and the general structure 1 of thiobenzoyl aminated compounds is:

Having the thiobenzoyl aminated compounds of above-mentioned formula 1, lepidoptera pest not only has high activity, and sucking pest (such as planthopper) is also had beyond thought high activity, this compounds has well double controlling and acts on and comprehensive function.The applicant is chlorine according to the preferred R1 of formula 1, and R2 is methyl, and with reference to the method in Chinese patent CN103109816A, the compound I (hereinafter referred to as Aminothiocarbonylbenzene) of the synthesis present invention, structural formula is as follows:

Although compound I (i.e. Aminothiocarbonylbenzene) is also the same with thiobenzoyl aminated compounds has prominent insecticidal effect.But its long-term single use, is easily generated Drug resistance, insecticidal activity declines.Finding through lot of experiments, Aminothiocarbonylbenzene is composite with triazole bactericidal agent, not only acts as potentiation, and also delays the pest and disease damage Drug resistance to Aminothiocarbonylbenzene and triazole bactericidal agent, improves prevention effect；Extend the service life of two kinds of medicaments simultaneously, reduce the development cost of new medicament.

Up to now, there is not yet the relevant report that Aminothiocarbonylbenzene is composite with triazole bactericidal agent agent.

Summary of the invention

It is an object of the invention to: for above-mentioned Problems existing, it is provided that a kind of composition pesticide composite by Aminothiocarbonylbenzene and triazole bactericidal agent, its pesticidal preparations and application thereof.This composition pesticide and pesticidal preparations thereof have obvious potentiation relative to single dose, can effectively preventing diseases and pests of agronomic crop, reduce the using dosage of pesticide, reduce cost accounting, while improving bactericidal and insecticidal activity, also delay the pest and disease damage Drug resistance to composition pesticide.

For realizing above-mentioned purpose, the technical scheme is that

Composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent; it is made up of Aminothiocarbonylbenzene and triazole bactericidal agent; wherein; Aminothiocarbonylbenzene is the bromo-N-of 3-[4-chloro-2-methyl-6-(methylamino thioformyl) benzene]-1-(3-chloropyridine-2-base)-1H-pyrazoles-5-Methanamide; it is 100:1～1:100 to ensure that composition pesticide has the weight ratio of better prevention effect and potentiation, Aminothiocarbonylbenzene and triazole bactericidal agent.

Preferably, the weight ratio of described Aminothiocarbonylbenzene and triazole bactericidal agent is 40:1～1:40.

It is further preferred that the weight ratio of described Aminothiocarbonylbenzene and triazole bactericidal agent is 20:1～1:20.

In the present invention, the structural formula of described Aminothiocarbonylbenzene is as follows:

Triazole bactericidal agent is organic heterocyclic compounds; have efficiently, the feature such as wide spectrum, low-residual, lasting period length, interior absorption be strong; having protection concurrently, treat, root out and fumigation action, except having significant preventing disease theraping effect, the growth of plant is also had stronger adjustment effect by triazole type medicament.Currently preferred triazole bactericidal agent is the one in Flutriafol, Tebuconazole, hexaconazole, Difenoconazole, epoxiconazole.It is preferred that, triazole bactericidal agent one in hexaconazole, Flutriafol, Tebuconazole.

Flutriafol (flutriafol), chemical name is α-(2-fluorophenyl)-α-(4-fluorophenyl)-1H-1,2,4-triazole-1-ethanol, it has the bactericidal activity of wide spectrum, its mechanism of action suppresses the biosynthesis of fungal cell's ergosterol with being characterized as being, thus causing cell wall rupture and suppressing the growth of mycelia.

Tebuconazole (tebuconazole): CAS 107534-96-3; chemical name is RS)-1-(4-chlorphenyl)-4; 4-dimethyl-3-(1H-1; 2; 4 triazol-1-yl methyl) penta-3-alcohol; being a kind of efficient, wide spectrum, interior absorption triazole bactericidal agent, have protection, treat, root out three zones, fungicidal spectrum is wide, the lasting period is long.

Hexaconazole (hexaconazole): CAS 79983-71-4; chemical name is (RS)-2-(2; 4-Dichlorobenzene base)-1-(1H-1; 2; 4-triazol-1-yl)-own-2-alcohol, the disease that fungus especially Basidiomycota and Ascomycota are caused has protection and the therapeutical effect of broad spectrum activity.

Difenoconazole (difenoconazole): CAS 119446-68-3; chemical name is suitable; the chloro-4-of trans-3-[4-methyl-2-1H-1; 2; 4-triazol-1-yl methyl)-1; mute pentane-2-the base of 3-bis-) phenyl 4-chlorphenyl ether, interior absorption sterilizes, and tool is protected and therapeutical effect.

Epoxiconazole (epoxiconazole): CAS 106325-08-0; chemical name is (2RS; 3RS)-1-[3-(2 monochloro-benzene base)-2; 3 one epoxy-2-(4 phenyl-monofluoride base) propyl group] one 1 hydrogen 1; 2; 4 one triazoles, BASF Aktiengesellschaft the triazole bactericidal agent developed, have protection and therapeutical effect concurrently.

The present invention also provides for a kind of pesticidal preparations preventing and treating agricultural pest, is made up of the above-mentioned composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent and adjuvant, and wherein, described composition pesticide accounts for the 0.1%～80% of described pesticidal preparations gross weight.It is further preferred that above-mentioned composition pesticide accounts for the 1%～50% of pesticidal preparations gross weight.

Above-mentioned adjuvant is the mixture of a kind of and auxiliary agent in solid carrier or liquid-carrier.Any carrier and the auxiliary agent that are generally used for composition pesticide all can use.

Solid carrier includes one or more in calcium magnesium phosphate, potassium chloride, chitin, carbamide, ammonium sulfate, Diammonium phosphate (DAP), sodium silicate, calcium superphosphate, humic acids, ammonium chloride, zinc sulfate, borax, montmorillonite, white carbon, precipitated calcium carbonate, kieselguhr, Kaolin, bentonite.

Liquid-carrier includes one or more in water, N-Methyl pyrrolidone, trimethylbenzene, two wires oil, methyl naphthalene, methyl oleate, C10 aromatic hydrocarbons, dimethylformamide, vegetable oil, Ketohexamethylene.

Above-mentioned auxiliary agent includes surfactant, wetting dispersing agent, also can add one or more in other conventional functionality auxiliary agents such as antifreezing agent, thickening agent, defoamer, disintegrating agent, film former if desired.

Wetting dispersing agent include NNO, sodium lauryl sulphate, dodecylbenzene sodium sulfonate, sodium lignin sulfonate, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid, pull open in powder, alkylbenzenesulfonate, calcium dodecyl benzene sulfonate, naphthalenesulfonate formaldehyde condensation compound, alkylphenol polyoxyethylene phosphate ester, polymeric polycarboxylic acid salt, alkyl benzene sulfonate calcium salt, polycarboxylate, alkylnaphthalene sulfonate, aliphatic amine polyoxyethylene ether, naphthalene sulfonic acid-formaldehyde condensation product sodium salt one or more.

Surfactant includes one or more in alkylphenol polyoxyethylene, calcium dodecyl benzene sulfonate, fatty alcohol-polyoxyethylene ether, NPE, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene, phenethyl phenol polyethenoxy ether, alkylaryl polyoxyethylene polyoxypropylene ether, styrylphenol polyoxyethylene ether, fatty alcohol-polyoxyethylene ether.

Film former includes carboxymethyl cellulose or methylcellulose.Antifreezing agent includes one or more in glycerol, propylene glycol, carbamide, ethylene glycol, isopropanol.Binding agent includes one or more in starch, sucrose, methylcellulose, ethyl cellulose, polyvinyl alcohol.Disintegrating agent includes sodium carbonate or sodium sulfate.Thickening agent includes one or more in xanthan gum, polyvinyl alcohol, aluminium-magnesium silicate, arabic gum.Defoamer is organosilicon.Warning coloration is carmine, Xylene Red or magenta.

Pesticidal preparations of the present invention can be configured to granule, seed treatment formulations, ultra low volume liquids, water dispersible granules, wettable powder or suspending agent according to method generally in the art.Wherein as preferably, the embodiment of seed treatment formulations provided by the invention is seed treatment dry powder doses, suspension seed-coating agent, seed treatment microcapsule suspending agent.

The composition pesticide of the present invention and pesticidal preparations can be used by commonsense method, and as spraying stem and leaf processes, it is possible to soil treatment, such as solid root spreads fertilizer over the fields or liquid pouring root, moreover it is possible to seed dressing, seed soaking or seed pelleting use.

The present invention also provides for the above-mentioned composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent and the application of above-mentioned a kind of pesticidal preparations preventing and treating agricultural pest, namely its application in controlling crop diseases and insect pests is referred to, such as insects such as the aphids on water rice hopper, wheat midge, various crop, the various diseases on the crop such as wheat sharp eyespot, rice sheath blight disease also can be prevented and treated.

Owing to adopting technique scheme, the invention have the benefit that

1, the composition pesticide of the present invention and pesticidal preparations thereof have obvious synergistic function, and Aminothiocarbonylbenzene is not simple superposition with triazole bactericidal agent combination.Toxicity Determination shows, they in certain proportion mixture co-toxicity coefficient more than 120.Equally, field medicine effect test result also indicates that, when effective ingredient dosage is fewer than single dose, preventing and treating wheat sharp eyespot, wheat midge, rice sheath blight disease prevention effect higher than single dose, improve prevention effect, decrease dosage, decrease using ecological harmful effect of pesticide, also reduce drug cost.

2, the composition pesticide of the present invention, Aminothiocarbonylbenzene is different from the triazole bactericidal agent mechanism of action, complementary action is played after composite, overcome the shortcoming that long-term single use easily produces resistance, slow down the pest and disease damage Drug resistance to both medicaments, extend the service life of medicament, simultaneously work as not only can parasite killing but also can cure the disease, strong adaptability.

Detailed description of the invention

The invention will be further described by the examples below.It should be noted that the embodiment of the present invention is only used for illustrating the present invention rather than limitation of the present invention.Aminothiocarbonylbenzene in following embodiment each means the material of its chemistry bromo-N-of 3-[4-chloro-2-methyl-6-(methylamino thioformyl) benzene]-1-(3-chloropyridine-2-base)-1H-pyrazoles-5-Methanamide by name; the former medicine of Aminothiocarbonylbenzene used is synthesized by laboratory, and the former medicine of other uses and preparation are commercial.

The potentiation composite for determining Aminothiocarbonylbenzene and triazole bactericidal agent, the applicant uses both compositions to carry out the Toxicity Determination of preventing and treating wheat sharp eyespot and rice sheath blight disease, and synergy adopts the abundant method of Sun Yun to calculate co-toxicity coefficient (CTC) and evaluates mixed effect.

Computational methods are:

The EC50*100 of the EC50/B agent of toxicity index TI (B)=standard agent A

The EC50*100 of the EC50/AB of actual toxicity index ATI (AB)=A

Theoretical toxicity index TTI (AB)=TI (A) * A percent+TI (B) the * B in mixture percent in mixture

Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) × 100

The percentage composition of B in the percentage composition+B medicament toxicity index × mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100

Evaluation criterion is: co-toxicity coefficient >=120 show as potentiation；Co-toxicity coefficient≤80 show as antagonism；80 < co-toxicity coefficient < 120 show as summation action.

Toxicity Determination embodiment one: Aminothiocarbonylbenzene is tested with hexaconazole, the composite Toxicity Determination to rice sheath blight disease of Flutriafol respectively.

Test method: " NYT1156.5-2006 farm-chemical indoor determination test rule antibacterial the 5th part: suppress Rhizoctonia solani Kuhn test Broad Bean Leaves method ".And press the abundant method calculating co-toxicity coefficient of Sun Yun, result is in Table 1～table 2.

Table 1 Aminothiocarbonylbenzene and the composite Toxicity Determination result to rice sheath blight disease of hexaconazole

Medicament	EC50(mg/L)	Actual measurement toxicity index	Theoretical poison index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	211.85	0.06	/	/
Hexaconazole (B)	0.12	100.00	/	/
					A:B=1:110	0.108	111.11	99.10	112.1
A:B=1:100	0.095	126.32	99.01	127.6
					A:B=1:90	0.089	134.83	98.90	136.3
A:B=1:80	0.088	136.36	98.77	138.1
					A:B=1:70	0.085	141.18	98.59	143.2
A:B=1:60	0.081	148.15	98.36	150.6
					A:B=1:50	0.077	155.84	98.04	159.0
A:B=1:40	0.071	169.01	97.56	173.2
					A:B=1:30	0.073	164.38	96.78	169.9
A:B=1:20	0.07	171.43	95.24	180.0
					A:B=1:10	0.068	176.47	90.91	194.1 4 -->
A:B=1:5	0.071	169.01	83.34	202.8
					A:B=1:3	0.083	144.58	75.01	192.7
A:B=1:1	0.136	88.24	50.03	176.4
					A:B=3:1	0.274	43.80	25.04	174.9
A:B=5:1	0.417	28.78	16.71	172.2

A:B=10:1	0.75	16.00	9.14	175.0
					A:B=20:1	1.51	7.95	4.82	165.0
A:B=30:1	2.24	5.36	3.28	163.3
					A:B=40:1	2.92	4.11	2.49	164.8
A:B=50:1	3.75	3.20	2.02	158.7
					A:B=60:1	4.86	2.47	1.70	145.7
A:B=70:1	5.75	2.09	1.46	142.5
					A:B=80:1	6.26	1.92	1.29	148.5
A:B=90:1	7.63	1.57	1.15	136.2
					A:B=100:1	9.33	1.29	1.05	122.9
A:B=110:1	12.48	0.96	0.96	100.5

From result of the test table 1: Aminothiocarbonylbenzene and hexaconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of rice sheath blight disease is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 160, it was shown that potentiation is extremely notable.

Table 2 Aminothiocarbonylbenzene and the composite indoor joint toxicity measuring result to rice sheath blight disease of Flutriafol

Medicament	EC50(mg/L)	Actual measurement toxicity index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	218.46	1.68	/	/
Flutriafol (C)	3.68	100.00	/	/
					A:C=1:110	3.14	117.20	99.11	118.2
A:C=1:100	2.95	124.75	99.03	126.0
					A:C=1:90	2.71	135.79	98.92	137.3
A:C=1:80	2.58	142.64	98.79	144.4
					A:C=1:70	2.55	144.31	98.62	146.3
A:C=1:60	2.48	148.39	98.39	150.8
					A:C=1:50	2.34	157.26	98.07	160.4
A:C=1:40	2.29	160.70	97.60	164.6
					A:C=1:30	2.24	164.29	96.83	169.7
A:C=1:20	2.17	169.59	95.32	177.9
					A:C=1:10	2.1	175.24	91.06	192.4
A:C=1:5	2.36	155.93	83.61	186.5
					A:C=1:2	3.05	120.66	67.23	179.5
A:C=1:1	3.98	92.46	50.84	181.9 5 -->
					A:C=5:1	11.17	32.95	18.07	182.3
A:C=10:1	19.7	18.68	10.62	175.9
					A:C=20:1	33.56	10.97	6.37	172.2
A:C=30:1	46.05	7.99	4.86	164.6
					A:C=40:1	54.08	6.80	4.08	166.7
A:C=50:1	65.75	5.60	3.61	154.9
					A:C=60:1	75.86	4.85	3.30	147.2
A:C=70:1	84.34	4.36	3.07	142.2
					A:C=80:1	85.26	4.32	2.90	148.9

A:C=90:1	97.63	3.77	2.76	136.3
					A:C=100:1	112.33	3.28	2.66	123.3
A:C=110:1	128.48	2.86	2.57	111.4

Result of the test from table 2: Aminothiocarbonylbenzene and Flutriafol by weight between 100:1～1:100 composite time the co-toxicity coefficient of rice sheath blight disease is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 164, it was shown that potentiation is extremely notable.

Toxicity Determination embodiment two: Aminothiocarbonylbenzene is tested with Tebuconazole, Difenoconazole, the composite Toxicity Determination to wheat sharp eyespot of epoxiconazole respectively.

Test method: adopt mycelial growth rate method to measure, Aminothiocarbonylbenzene and triazole bactericidal agent are diluted to sterilized water respectively the medicinal liquid of variable concentrations, it is proportionally added in the PDA culture medium after melting, make the flat board containing variable concentrations antibacterial, by prior cultured wheat sharp eyespot pathogenic bacteria bacterium cake (7mm is placed in flat board central authorities), on the basis of pilot study, every medicament arranges several variable concentrations gradient, often process 4 times and repeat, with the culture medium flat plate of not pastille for comparison.Postvaccinal culture dish is placed in 25 DEG C and cultivates 4d, then measure colony diameter, thus calculate the mycelial growth inhibition rate of each process.And press the abundant method calculating co-toxicity coefficient of Sun Yun.Result is in Table 3～table 5.

Table 3 Aminothiocarbonylbenzene and the composite indoor joint toxicity measuring result to wheat sharp eyespot of Tebuconazole

Medicament	EC50(mg/L)	Actual measurement toxicity index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	215.58	0.40	/	/
Tebuconazole (D)	0.86	100.00	/	/
					A:D=1:110	0.75	114.67	99.10	115.7
A:D=1:100	0.67	128.36	99.01	129.6
					A:D=1:90	0.63	136.51	98.91	138.0
A:D=1:80	0.61	140.98	98.77	142.7
					A:D=1:70	0.58	148.28	98.60	150.4
A:D=1:60	0.57	150.88	98.37	153.4
					A:D=1:50	0.57	150.88	98.05	153.9
A:D=1:40	0.53	162.26	97.57	166.3
					A:D=1:30	0.52	165.38	96.79	170.9
A:D=1:20	0.5	172.00	95.26	180.6 6 -->
					A:D=1:10	0.48	179.17	90.95	197.0
A:D=1:4	0.57	150.88	80.08	188.4
					A:D=1:1	0.95	90.53	50.20	180.3
A:D=4:1	2.28	37.72	20.32	185.6

A:D=10:1	5.16	16.67	9.45	176.3
					A:D=20:1	9.39	9.16	5.14	178.1
A:D=30:1	14.45	5.95	3.61	164.8
					A:D=40:1	17.95	4.79	2.83	169.4
A:D=50:1	23.81	3.61	2.35	153.6
					A:D=60:1	26.86	3.20	2.03	157.6
A:D=70:1	30.55	2.82	1.80	156.2
					A:D=80:1	35.47	2.42	1.63	148.9
A:D=90:1	39.82	2.16	1.49	144.6
					A:D=100:1	45.28	1.90	1.39	137.1
A:D=110:1	58.64	1.47	1.30	113.1

From result of the test table 3: Aminothiocarbonylbenzene and Tebuconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat sharp eyespot is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 164, it was shown that potentiation is extremely notable.

Table 4 Aminothiocarbonylbenzene and the composite indoor joint toxicity measuring result to wheat sharp eyespot of epoxiconazole

Medicament	EC50(mg/L)	Actual measurement toxicity index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	213.66	0.44	/	/
Epoxiconazole (E)	0.93	100.00	/	/
					A:E=1:110	0.91	102.20	99.10	103.1
A:E=1:100	0.76	122.37	99.01	123.6
					A:E=1:90	0.67	138.81	98.91	140.3
A:E=1:80	0.62	150.00	98.77	151.9
					A:E=1:70	0.59	157.63	98.60	159.9
A:E=1:60	0.60	155.00	98.37	157.6
					A:E=1:50	0.63	147.62	98.05	150.6
A:E=1:40	0.54	172.22	97.57	176.5
					A:E=1:30	0.51	182.35	96.79	188.4
A:E=1:20	0.51	182.35	95.26	191.4
					A:E=1:9	0.49	189.80	90.04	210.8
A:E=1:4	0.59	157.63	80.09	196.8
					A:E=1:1	0.98	94.90	50.22	189.0
A:E=4:1	2.39	38.91	20.35	191.2
					A:E=9:1	5.18	17.95	10.39	172.8 7 -->
A:E=20:1	10.21	9.11	5.18	176.0
					A:E=30:1	14.38	6.47	3.65	177.3
A:E=40:1	18.74	4.96	2.86	173.3
					A:E=50:1	25.85	3.60	2.39	150.7
A:E=60:1	28.92	3.22	2.07	155.5
					A:E=70:1	34.46	2.70	1.84	146.9
A:E=80:1	38.54	2.41	1.66	145.0
					A:E=90:1	43.8	2.12	1.53	138.8

A:E=100:1	48.19	1.93	1.42	135.8
					A:E=110:1	61.34	1.52	1.33	113.8

From result of the test table 4: Aminothiocarbonylbenzene and epoxiconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat sharp eyespot is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 170, it was shown that potentiation is extremely notable.

Table 5 Aminothiocarbonylbenzene and the composite indoor joint toxicity measuring result to wheat sharp eyespot of Difenoconazole

Medicament	EC50(mg/L)	Actual measurement toxicity index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	216.23	0.86	/	/
Difenoconazole (F)	1.85	100.00	/	/
					A:F=1:110	1.76	105.11	99.11	106.1
A:F=1:100	1.44	128.47	99.02	129.7
					A:F=1:90	1.41	131.21	98.91	132.7
A:F=1:80	1.35	137.04	98.78	138.7
					A:F=1:70	1.27	145.67	98.60	147.7
A:F=1:60	1.21	152.89	98.37	155.4
					A:F=1:50	1.19	155.46	98.06	158.5
A:F=1:40	1.12	165.18	97.58	169.3
					A:F=1:30	1.04	177.88	96.80	183.8
A:F=1:20	1.07	172.90	95.28	181.5
					A:F=1:10	1.08	171.30	90.99	188.3
A:F=1:4	1.21	152.89	80.17	190.7
					A:F=1:1	1.98	93.43	50.43	185.3
A:F=4:1	4.56	40.57	20.68	196.1
					A:F=10:1	9.6	19.27	9.87	195.3
A:F=20:1	18.35	10.08	5.58	180.8
					A:F=30:1	27.14	6.82	4.05	168.2
A:F=40:1	33.87	5.46	3.27	166.8
					A:F=50:1	41.7	4.44	2.80	158.5
A:F=60:1	46.3	4.00	2.48	161.1 8 -->
					A:F=70:1	55.64	3.32	2.25	147.6
A:F=80:1	65.21	2.84	2.08	136.4
					A:F=90:1	70.8	2.61	1.95	134.3
A:F=100:1	81.14	2.28	1.84	124.1
					A:F=110:1	98.07	1.89	1.75	107.9

From result of the test table 5: Aminothiocarbonylbenzene and Difenoconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat sharp eyespot is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 166, it was shown that potentiation is extremely notable.

Indoor bioassay test example three: Aminothiocarbonylbenzene respectively with hexaconazole, Flutriafol, Tebuconazole, the composite indoor joint toxicity measuring to wheat midge of epoxiconazole.Synergy adopts the abundant method of Sun Yun to calculate co-toxicity coefficient (CTC) and evaluates mixed effect.

Computational methods are:

Actual measurement toxicity index (ATI)=(standard agent LC50/ reagent agent LC50) × 100

The percentage composition of B in the percentage composition+B medicament toxicity index × mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100

Evaluation criterion: when CTC≤80, then compositions shows as antagonism, and as 80 < CTC < 120, then compositions shows as summation action, and when CTC >=120, then compositions shows as potentiation.

Test method: adopt infusion process to measure, medicament acetone is diluted to variable concentrations, the midge worm larva that picking is neat, physiological status is consistent is tested, take out after examination worm is impregnated 30s in the funnel that the filter paper equipped with medicament is made, suck unnecessary medicinal liquid with absorbent paper, put in the insect box equipped with moistening filter paper.Use clear water and cosolvent as comparison respectively.Often process test no less than 60, repeat 3 times.It is 20 DEG C that insect box is placed in temperature, and relative air humidity is in the temperature of about 80%.After 3d and 5d, the death condition of checkout facility, and calculate corrected mortality, death standard shrinks with polypide or can not normally creep.Adopt the abundant method of Sun Yun to calculate co-toxicity coefficient (CTC) and evaluate mixed effect.

Table 6 Aminothiocarbonylbenzene and the composite joint toxicity measuring result to wheat midge of hexaconazole

Medicament	LC50(mg/L)	Actual measurement toxicity index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	8.43	100.00	/	/
Hexaconazole (B)	184.67	4.56	/	/
					A:B=110:1	8.21	102.68	99.14	103.6
A:B=100:1	6.84	123.25	99.06	124.4
					A:B=90:1	6.53	129.10	98.95	130.5
A:B=80:1	6.22	135.53	98.82	137.1
					A:B=70:1	6.05	139.34	98.66	141.2
A:B=60:1	5.89	143.12	98.44	145.4

A:B=50:1	5.78	145.85	98.13	148.6
					A:B=40:1	5.54	152.17	97.67	155.8
A:B=30:1	5.35	157.57	96.92	162.6 9 -->
					A:B=20:1	5.08	165.94	95.46	173.8
A:B=9:1	4.76	177.10	90.46	195.8
					A:B=5:1	5.24	160.88	84.09	191.3
A:B=2:1	6.58	128.12	68.19	187.9
					A:B=1:1	9.12	92.43	52.28	176.8
A:B=1:4	21.25	39.67	23.65	167.7
					A:B=1:9	36.68	22.98	14.11	162.9
A:B=1:20	57.45	14.67	9.11	161.1
					A:B=1:30	70.39	11.98	7.64	156.7
A:B=1:40	79.54	10.60	6.89	153.8
					A:B=1:50	88.68	9.51	6.44	147.7
A:B=1:60	94.62	8.91	6.13	145.4
					A:B=1:70	102.38	8.23	5.91	139.3
A:B=1:80	110.58	7.62	5.74	132.7
					A:B=1:90	118.73	7.10	5.61	126.5
A:B=1:100	125.18	6.73	5.51	122.2

From result of the test table 6: Aminothiocarbonylbenzene and hexaconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat midge is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 150, it was shown that potentiation is extremely notable.

Table 7 Aminothiocarbonylbenzene and the composite joint toxicity measuring result to wheat midge of Flutriafol

Medicament	LC50(mg/L)	Actual measurement poison index	Theoretical poison index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	8.6	100.00	/	/
Flutriafol (C)	176.58	4.87	/	/
					A:C=110:1	8.34	103.12	99.14	104.0
A:C=100:1	6.72	127.98	99.06	129.2
					A:C=90:1	6.47	132.92	98.95	134.3
A:C=80:1	6.18	139.16	98.83	140.8
					A:C=70:1	6.01	143.09	98.66	145.0
A:C=60:1	5.83	147.51	98.44	149.8
					A:C=50:1	5.95	144.54	98.13	147.3
A:C=40:1	5.51	156.08	97.68	159.8
					A:C=30:1	5.32	161.65	96.93	166.8
A:C=20:1	5.04	170.63	95.47	178.7
					A:C=10:1	4.81	178.79	91.35	195.7
A:C=5:1	5.18	166.02	84.15	197.3
					A:C=3:1	6.14	140.07	76.22	183.8
A:C=1:1	9.08	94.71	52.44	180.6
					A:C=1:5	24.16	35.60	20.73	171.8

A:C=1:10	38.62	22.27	13.52	164.7
					A:C=1:20	57.37	14.99	9.40	159.5
A:C=1:30	69.23	12.42	7.94	156.5
					A:C=1:40	77.56	11.09	7.19	154.2
A:C=1:50	85.56	10.05	6.74	149.2
					A:C=1:60	92.47	9.30	6.43	144.6
A:C=1:70	100.11	8.59	6.21	138.3
					A:C=1:80	105.24	8.17	6.04	135.2
A:C=1:90	112.34	7.66	5.92	129.4
					A:C=1:100	119.38	7.20	5.81	123.9
A:C=1:110	134.45	6.40	5.73	111.7

From result of the test table 7: Aminothiocarbonylbenzene and Flutriafol by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat midge is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 154, it was shown that potentiation is extremely notable.

Table 8 Aminothiocarbonylbenzene and the composite joint toxicity measuring result to wheat midge of Tebuconazole

Medicament	LC50(mg/L)	Actual measurement poison index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	8.26	100.00	/	/
Tebuconazole (D)	198.52	4.16	/	/
					A:D=110:1	7.16	115.36	99.14	116.4
A:D=100:1	6.85	120.58	99.05	121.7
					A:D=90:1	6.56	125.91	98.95	127.3
A:D=80:1	6.43	128.46	98.82	130.0
					A:D=70:1	6.37	129.67	98.65	131.4
A:D=60:1	6.12	134.97	98.43	137.1
					A:D=50:1	5.93	139.29	98.12	142.0
A:D=40:1	5.62	146.98	97.66	150.5
					A:D=30:1	5.45	151.56	96.91	156.4
A:D=20:1	5.31	155.56	95.44	163.0
					A:D=10:1	4.75	173.89	91.29	190.5
A:D=3:1	6.36	129.87	76.04	170.8
					A:D=1:1	9.25	89.30	52.08	171.5
A:D=1:3	16.83	49.08	28.12	174.5
					A:D=1:10	38.84	21.27	12.87	165.2
A:D=1:20	57.51	14.36	8.72	164.6
					A:D=1:30	71.36	11.58	7.25	159.6
A:D=1:40	82.45	10.02	6.50	154.2
					A:D=1:50	93.78	8.81	6.04	145.8 11 -->
A:D=1:60	102.36	8.07	5.73	140.8
					A:D=1:70	108.75	7.60	5.51	137.8
A:D=1:80	112.68	7.33	5.34	137.2
					A:D=1:90	126.59	6.53	5.21	125.1

A:D=1:100	131.94	6.26	5.11	122.5
					A:D=1:110	145.76	5.67	5.02	112.8

From result of the test table 8: Aminothiocarbonylbenzene and Tebuconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat midge is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 150, it was shown that potentiation is extremely notable.

Table 9 Aminothiocarbonylbenzene and the composite joint toxicity measuring result to wheat midge of epoxiconazole

Medicament	LC50(mg/L)	Actual measurement toxicity index	Theoretical toxicity index	Co-toxicity coefficient
					Aminothiocarbonylbenzene (A)	8.38	100.00	/	/
Epoxiconazole (E)	205.47	4.08	/	/
					A:E=110:1	8.25	101.58	99.14	102.5
A:E=100:1	6.57	127.55	99.05	128.8
					A:E=90:1	6.54	128.13	98.95	129.5
A:E=80:1	6.26	133.87	98.82	135.5
					A:E=70:1	5.93	141.32	98.65	143.3
A:E=60:1	6.11	137.15	98.43	139.3
					A:E=50:1	5.78	144.98	98.12	147.8
A:E=40:1	5.25	159.62	97.66	163.4
					A:E=30:1	4.96	168.95	96.91	174.3
A:E=20:1	4.47	187.47	95.43	196.4
					A:E=10:1	5.13	163.35	91.28	179.0
A:E=9:1	5.4	155.19	90.41	171.7
					A:E=4:1	6.14	136.48	80.82	168.9
A:E=1:1	9.64	86.93	52.04	167.0
					A:E=1:4	21.28	39.38	23.26	169.3
A:E=1:10	39.26	21.34	12.80	166.8
					A:E=1:20	59.73	14.03	8.65	162.3
A:E=1:30	71.41	11.74	7.17	163.6
					A:E=1:40	80.75	10.38	6.42	161.7
A:E=1:50	95.47	8.78	5.96	147.3
					A:E=1:60	105.63	7.93	5.65	140.4
A:E=1:70	109.28	7.67	5.43	141.2
					A:E=1:80	114.57	7.31	5.26	139.0
A:E=1:90	118.64	7.06	5.13	137.6 12 -->
					A:E=1:100	131.35	6.38	5.03	126.9
A:E=1:110	148.76	5.63	4.94	114.0

From result of the test table 9: Aminothiocarbonylbenzene and epoxiconazole by weight between 100:1～1:100 composite time the co-toxicity coefficient of wheat midge is all higher than 120, show as potentiation.Especially proportioning is within the scope of 40:1～1:40, and each co-toxicity coefficient processed is all higher than 161, it was shown that potentiation is extremely notable.

Formulation Example:

Below in conjunction with specific embodiment, the present invention is further described, but does not limit the invention to these detailed description of the invention.For the purpose of unification, embodiment set forth below, dispersant and emulsifying agent all adopt abbreviation or commercial disignation；Percentage ratio in formula is all weight percentage；CK1-CK24 is comparative example.

One, the embodiment of granule

Manufacture method: by formula proportion by Aminothiocarbonylbenzene, triazole bactericidal agent, Ricinate, binding agent, carrier mix homogeneously, pelletize the granule of pesticidal preparations of the present invention.

Embodiment 10.4% Aminothiocarbonylbenzene hexaconazole granule (1:1)

Aminothiocarbonylbenzene 0.2%, hexaconazole 0.2%, NNO2%, sodium lauryl sulphate 1%, calcium magnesium phosphate phosphorus 3%, potassium chloride 1%, white carbon 2%, chitin 1.5%, carbamide supplies 100%.

Embodiment 20.1% Aminothiocarbonylbenzene hexaconazole granule (1:1)

Aminothiocarbonylbenzene 0.05%, hexaconazole 0.05%, sodium lignin sulfonate 1.5%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 1%, ammonium sulfate 3%, Diammonium phosphate (DAP) 10%, potassium chloride 10%, starch 1%, carbamide supplies 100%.

Embodiment 35% Aminothiocarbonylbenzene hexaconazole granule (4:1)

Aminothiocarbonylbenzene 4%, hexaconazole 1%, sodium lignin sulfonate 2%, pull open powder 1.2%, ammonium sulfate 3%, Diammonium phosphate (DAP) 10%, starch 1%, carbamide 20%, borax supplies 100%.

CK1:1% Aminothiocarbonylbenzene granule: Aminothiocarbonylbenzene 1%, NNO2%, sodium lauryl sulphate 1%, calcium magnesium phosphate phosphorus 3%, potassium chloride 1%, white carbon 2%, chitin 1.5%, carbamide supplies 100%.

CK2:1% hexaconazole granule: hexaconazole 1%, NNO2%, sodium lauryl sulphate 1%, calcium magnesium phosphate phosphorus 3%, potassium chloride 1%, white carbon 2%, chitin 1.5%, carbamide supplies 100%.

Embodiment 40.3% Aminothiocarbonylbenzene Flutriafol granule (1:2)

Aminothiocarbonylbenzene 0.1%, Flutriafol 0.2%, calcium lignosulfonate 1%, pull open powder 2%, calcium magnesium phosphate 3%, ammonium sulfate 3%, Diammonium phosphate (DAP) 6%, white carbon 1.5%, humic acids 10%, carbamide supplies 100%.

Embodiment 55% Aminothiocarbonylbenzene Flutriafol granule (1:1)

Aminothiocarbonylbenzene 2.5%, Flutriafol 2.5%, NNO3%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 1%, potassium chloride 3%, Diammonium phosphate (DAP) 6%, white carbon 1.5%, montmorillonite supplies 100%.

CK3:1% Flutriafol granule: Flutriafol 1%, NNO3%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 1%, potassium chloride 3%, Diammonium phosphate (DAP) 6%, white carbon 1.5%, montmorillonite supplies 100%.

Embodiment 60.6% Aminothiocarbonylbenzene Tebuconazole granule (1:1)

Aminothiocarbonylbenzene 0.3%, Tebuconazole 0.3%, sodium lignin sulfonate 1%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 2%, potassium chloride 3%, ammonium chloride 6%, zinc sulfate 1%, starch 1.5%, carbamide supplies 100%.

CK4:1% Tebuconazole granule: Tebuconazole 1%, sodium lignin sulfonate 1%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 2%, potassium chloride 3%, ammonium chloride 6%, zinc sulfate 1%, starch 1.5%, carbamide supplies 100%

Embodiment 711% Aminothiocarbonylbenzene Tebuconazole granule (1:10)

Aminothiocarbonylbenzene 1%, Tebuconazole 10%, sodium lignin sulfonate 2.5%, alkylbenzenesulfonate 1%, potassium chloride 2.5%, calcium magnesium phosphate 3%, Diammonium phosphate (DAP) 2%, white carbon 2%, carbamide 20%, kieselguhr supplies 100%.

Embodiment 80.5% Aminothiocarbonylbenzene Difenoconazole granule (4:1)

Aminothiocarbonylbenzene 0.4%, Difenoconazole 0.1%, sodium lignin sulfonate 2.5%, calcium dodecyl benzene sulfonate 0.5%, potassium chloride 2.5%, calcium magnesium phosphate 3%, sodium silicate 1%, sucrose 1%, carbamide 20%, kieselguhr supplies 100%.

CK5:1% Difenoconazole granule: Difenoconazole 1%, sodium lignin sulfonate 2.5%, calcium dodecyl benzene sulfonate 0.5%, potassium chloride 2.5%, calcium magnesium phosphate 3%, sodium silicate 1%, sucrose 1%, carbamide 20%, kieselguhr supplies 100%.

Embodiment 91% Aminothiocarbonylbenzene epoxiconazole granule (4:1)

Aminothiocarbonylbenzene 0.8%, epoxiconazole 0.2%, NNO3%, alkylbenzenesulfonate 1%, potassium chloride 2.5%, calcium magnesium phosphate 3%, zinc sulfate 1%, calcium superphosphate 1%, white carbon 5%, carbamide supplies 100%.

CK6:1% epoxiconazole granule (0.8:0.2): epoxiconazole 1%, NNO3%, alkylbenzenesulfonate 1%, potassium chloride 2.5%, calcium magnesium phosphate 3%, zinc sulfate 1%, calcium superphosphate 1%, white carbon 5%, carbamide supplies 100%.

Two, the preparation of seed treatment formulations

(1) suspension seed-coating agent preparation technology: by formula proportion by Aminothiocarbonylbenzene and triazole bactericidal agent, surfactant or wetting dispersing agent mix homogeneously, it is subsequently adding suitable quantity of water, use sand mill wet grinding, then transfer the material in cutting kettle, add film former, thickening agent, antifreezing agent, defoamer, warning coloration and remaining water, through high speed shear to viscosity, particle diameter conformance with standard, obtain the suspension seed-coating agent of pesticidal preparations of the present invention.

Embodiment 1010% Aminothiocarbonylbenzene hexaconazole suspension seed-coating agent (1:9)

Aminothiocarbonylbenzene 1%, hexaconazole 9k%, naphthalenesulfonate formaldehyde condensation compound 2.5%, sodium lignin sulfonate 2%, carboxymethyl cellulose 2%, xanthan gum 1.5%, organosilicon 0.05%, glycerol 2%, carmine 0.2%, water is mended to 100%.

Embodiment 118% Aminothiocarbonylbenzene Flutriafol suspension seed-coating agent (3:1)

Aminothiocarbonylbenzene 2%, Flutriafol 5%, NNO2%, alkylphenol polyoxyethylene phosphate ester 1%, sodium lignin sulfonate 1%, polyvinyl alcohol 2%, propylene glycol 2%, Xylene Red 0.5%, water supplies 100%.

Embodiment 1216% Aminothiocarbonylbenzene Tebuconazole suspension seed-coating agent (3:1)

Aminothiocarbonylbenzene 12%, Tebuconazole 4%, NNO1%, sodium lignin sulfonate 1.5%, alkylbenzenesulfonate 1%, methylcellulose 2%, glycerol 2%, azogeramine %, water supplies 100%.

Embodiment 135% Aminothiocarbonylbenzene difenoconazole suspending seed-dressing agent (1:1)

Aminothiocarbonylbenzene 2.5%, Difenoconazole 2.5%, polymeric polycarboxylic acid salt 3%, sodium lauryl sulphate 1%, polyvinyl alcohol 2%, glycerol 2%, carmine 0.4%, water supplies 100%.

Embodiment 1411% Aminothiocarbonylbenzene epoxiconazole suspension seed-coating agent (1:10)

Aminothiocarbonylbenzene 1%, epoxiconazole 10%, NNO3%, alkylbenzenesulfonate 1%, methylcellulose 2.5%, glycerol 2%, carmine 0.4%, organosilicon 0.1%, water supplies 100%.

(2) preparation technology of seed treatment dry powder doses:

By formula proportion by Aminothiocarbonylbenzene, triazole bactericidal agent, surfactant or wetting dispersing agent, adding film former, binding agent, warning coloration and solid carrier carry out pulverizing and mix homogeneously, obtain the seed treatment dry powder doses of pesticidal preparations of the present invention.

Embodiment 1515% Aminothiocarbonylbenzene hexaconazole seed treatment dry powder doses (2:1)

Aminothiocarbonylbenzene 10%, hexaconazole 5%, sodium lignin sulfonate 3%, alkylbenzenesulfonate 1%, methylcellulose 2.5%, azogeramine %, white carbon 5%, Kaolin supplies 100%.

CK7:5% Aminothiocarbonylbenzene seed treatment dry powder doses: Aminothiocarbonylbenzene 5%, sodium lignin sulfonate 3%, alkylbenzenesulfonate 1%, methylcellulose 2.5%, azogeramine %, white carbon 5%, Kaolin supplies 100%.

CK8:5% hexaconazole seed treatment dry powder doses: hexaconazole 5%, sodium lignin sulfonate 3%, alkylbenzenesulfonate 1%, methylcellulose 2.5%, azogeramine %, white carbon 5%, Kaolin supplies 100%.

Embodiment 1612% Aminothiocarbonylbenzene Flutriafol seed treatment dry powder doses (1:5)

Aminothiocarbonylbenzene 2%, Flutriafol 10%, polymeric polycarboxylic acid salt 3k%, sodium lauryl sulphate 2.5k%, polyvinyl alcohol 3%, carmine 1%, white carbon 5%, kieselguhr supplies 100%.

CK9:5% Flutriafol seed treatment dry powder doses: Flutriafol 5%, polymeric polycarboxylic acid salt 3%, sodium lauryl sulphate 2.5%, polyvinyl alcohol 3%, carmine 1%, white carbon 5%, kieselguhr supplies 100%.

Embodiment 178% Aminothiocarbonylbenzene Tebuconazole seed treatment dry powder doses (1:3)

Aminothiocarbonylbenzene 2%, Tebuconazole 6%, naphthalenesulfonate formaldehyde condensation compound 2%, dodecylbenzene sodium sulfonate 2%, carboxymethyl cellulose 3%, carmine 0.8%, kieselguhr 10%, white carbon 2%, Kaolin supplies 100%.

CK10:5% Tebuconazole seed treatment dry powder doses: Tebuconazole 5%, naphthalenesulfonate formaldehyde condensation compound 2%, dodecylbenzene sodium sulfonate 2%, carboxymethyl cellulose 3%, carmine 0.8%, kieselguhr 10%, white carbon 2%, Kaolin supplies 100%.

Embodiment 1811% Aminothiocarbonylbenzene Difenoconazole seed treatment dry powder doses (1:10)

Aminothiocarbonylbenzene 1%, Difenoconazole 10%, naphthalenesulfonate formaldehyde condensation compound 1%, dodecylbenzene sodium sulfonate 2.52%, methylcellulose 2.6%, carmine 0.6%, white carbon 2%, precipitated calcium carbonate 5%, Kaolin supplies 100%.

CK11:5% Difenoconazole seed treatment dry powder doses: Difenoconazole 5%, naphthalenesulfonate formaldehyde condensation compound 1%, dodecylbenzene sodium sulfonate 2.52%, methylcellulose 2.6%, carmine 0.6%, white carbon 2%, precipitated calcium carbonate 5%, Kaolin supplies 100%.

Embodiment 1910% Aminothiocarbonylbenzene epoxiconazole seed treatment dry powder doses (9:1)

Aminothiocarbonylbenzene 9%, epoxiconazole 1%, sodium lignin sulfonate 2%, alkylbenzenesulfonate 3%, dodecylbenzene sodium sulfonate 1%, methylcellulose 2%, carmine 0.5%, starch 3%, white carbon 10%, bentonite supplies 100%.

CK12:1% epoxiconazole seed treatment dry powder doses: epoxiconazole 1%, sodium lignin sulfonate 2%, alkylbenzenesulfonate 3%, dodecylbenzene sodium sulfonate 1%, methylcellulose 2%, carmine 0.5%, starch 3%, white carbon 10%, bentonite supplies 100%.

Three, the preparation of ultra low volume liquids

The preparation technology of ultra low volume liquids: by formula proportion by Aminothiocarbonylbenzene, triazole bactericidal agent, surfactant or wetting dispersing agent, surfactant carries out mixing to former medicine and is completely dissolved into transparency liquid with liquid-carrier, obtains the ultra low volume liquids of pesticidal preparations of the present invention.

Embodiment 2011% Aminothiocarbonylbenzene hexaconazole ultra low volume liquids (10:1)

Aminothiocarbonylbenzene 10%, hexaconazole 1%, N-Methyl pyrrolidone 15%, trimethylbenzene 10%, alkylphenol polyoxyethylene 3%, calcium dodecyl benzene sulfonate 1%, two wires oil supplies 100%.

CK13:5% Aminothiocarbonylbenzene ultra low volume liquids: Aminothiocarbonylbenzene 5%, N-Methyl pyrrolidone 15%, trimethylbenzene 10%, alkylphenol polyoxyethylene 3%, calcium dodecyl benzene sulfonate 1%, two wires oil supplies 100%.

CK14:1% hexaconazole ultra low volume liquids: hexaconazole 1%, N-Methyl pyrrolidone 15%, trimethylbenzene 10%, alkylphenol polyoxyethylene 3%, calcium dodecyl benzene sulfonate 1%, two wires oil supplies 100%

Embodiment 218% Aminothiocarbonylbenzene hexaconazole ultra low volume liquids (1:3)

Aminothiocarbonylbenzene 2%, hexaconazole 6%, N-Methyl pyrrolidone 10%, methyl naphthalene 10%, fatty alcohol-polyoxyethylene ether 2%, calcium dodecyl benzene sulfonate 2%, methyl oleate supplies 100%.

Embodiment 228.4% Aminothiocarbonylbenzene Flutriafol ultra low volume liquids (1:20)

Aminothiocarbonylbenzene 0.4%, Flutriafol 8%, N-Methyl pyrrolidone 15%, trimethylbenzene 10%, NPE 1%, castor oil polyoxyethylene ether 2%, calcium dodecyl benzene sulfonate 1.5%, C10 aromatic hydrocarbons supplies 100%.

CK15:1% Flutriafol ultra low volume liquids: Flutriafol 1%, N-Methyl pyrrolidone 15%, trimethylbenzene 10%, NPE 1%, castor oil polyoxyethylene ether 2%, calcium dodecyl benzene sulfonate 1.5%, C10 aromatic hydrocarbons supplies 100%.

Embodiment 236.2% Aminothiocarbonylbenzene Tebuconazole ultra low volume liquids (30:1)

Aminothiocarbonylbenzene 6%, Tebuconazole 0.2%, N-Methyl pyrrolidone 4%, dimethylformamide 8%, alkylphenol polyoxyethylene 3%, calcium dodecyl benzene sulfonate 2%, vegetable oil supplies 100%.

CK16:1% Tebuconazole ultra low volume liquids: Tebuconazole 1%, N-Methyl pyrrolidone 4%, dimethylformamide 8%, alkylphenol polyoxyethylene 3%, calcium dodecyl benzene sulfonate 2%, vegetable oil supplies 100%.

Embodiment 246.2% Aminothiocarbonylbenzene epoxiconazole ultra low volume liquids (1:30)

Aminothiocarbonylbenzene 0.2%, epoxiconazole 6%, N-Methyl pyrrolidone 4%, dimethylformamide 8%, alkylphenol polyoxyethylene 3%, calcium dodecyl benzene sulfonate 2%, vegetable oil supplies 100%.

Embodiment 256% Aminothiocarbonylbenzene epoxiconazole ultra low volume liquids (5:1)

Aminothiocarbonylbenzene 5%, epoxiconazole 1%, N-Methyl pyrrolidone 10%, methyl oleate 5%, phenethyl phenol polyethenoxy ether 1.5%, alkylaryl polyoxyethylene polyoxypropylene ether 3%, two wires oil supplies 100%.

CK17:1% epoxiconazole ultra low volume liquids: epoxiconazole 1%, N-Methyl pyrrolidone 10%, methyl oleate 5%, phenethyl phenol polyethenoxy ether 1.5%, alkylaryl polyoxyethylene polyoxypropylene ether 3%, two wires oil supplies 100%.

Embodiment 2610% Aminothiocarbonylbenzene Difenoconazole ultra low volume liquids (4:1)

Aminothiocarbonylbenzene 8%, Difenoconazole 2%, N-Methyl pyrrolidone 5%, Ketohexamethylene 5%, castor oil polyoxyethylene ether 1%, alkylaryl polyoxyethylene polyoxypropylene ether 3%, trimethylbenzene supplies 100%

CK18:1% Difenoconazole ultra low volume liquids: Difenoconazole 1%, N-Methyl pyrrolidone 5%, Ketohexamethylene 5%, castor oil polyoxyethylene ether 1%, alkylaryl polyoxyethylene polyoxypropylene ether 3%, trimethylbenzene supplies 100%.

Four, the preparation of wettable powder

Preparation technology: by effective ingredient Aminothiocarbonylbenzene, triazole bactericidal agent, dispersant, wetting agent, solid carrier mix homogeneously, after jet mill is pulverized, stirs 30min, obtains the wettable powder of pesticidal preparations of the present invention.

Embodiment 27:41% Aminothiocarbonylbenzene hexaconazole wettable powder (40:1)

Aminothiocarbonylbenzene 40%, hexaconazole 1%, NNO4%, sodium lauryl sulphate 2%, white carbon 10%, kieselguhr supplies 100%.

Embodiment 28:50% Aminothiocarbonylbenzene hexaconazole wettable powder (1:4)

By Aminothiocarbonylbenzene 10%, hexaconazole 40%, pulling open powder 1%, NNO3%, sodium lauryl sulphate 1%, Kaolin supplies 100%.

Embodiment 29:10.5% Aminothiocarbonylbenzene hexaconazole wettable powder (1:20)

By Aminothiocarbonylbenzene 0.5%, hexaconazole 10%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 2%, NNO1%, sodium lauryl sulphate 1%, Kaolin supplies 100%.

Embodiment 30:20.5% Aminothiocarbonylbenzene Flutriafol wettable powder (40:1)

By Aminothiocarbonylbenzene 20%, Flutriafol 0.5%, NNO3%, alkylnaphthalene sulfonate 2%, white carbon 10%, bentonite supplies 100%.

Embodiment 31:22% Aminothiocarbonylbenzene Flutriafol wettable powder (1:10)

By Aminothiocarbonylbenzene 2%, Flutriafol 20%, NNO2%, sodium lignin sulfonate 2%, alkyl benzene sulfonate calcium salt 1%, white carbon 5%, Kaolin supplies 100%.

Embodiment 32:21% Aminothiocarbonylbenzene Tebuconazole wettable powder (1:20)

By Aminothiocarbonylbenzene 1%, Tebuconazole 20%, polycarboxylate 3%, sodium lignin sulfonate 2%, white carbon 5%, Kaolin supplies 100%.

Embodiment 33:12% Aminothiocarbonylbenzene epoxiconazole wettable powder (1:5)

By Aminothiocarbonylbenzene 2%, epoxiconazole 10%, alkylnaphthalene sulfonate 3.5%, sodium lauryl sulphate 1%, white carbon 18%, Kaolin supplies 100%.

Embodiment 34:31% Aminothiocarbonylbenzene Difenoconazole wettable powder (1:30)

By Aminothiocarbonylbenzene 1%, Difenoconazole 30%, alkylnaphthalene sulfonate 3.5%, polycarboxylate 0.5%, sodium lauryl sulphate 1%, Kaolin supplies 100%.

Five, the preparation of water dispersible granules

Preparation technology: by effective ingredient Aminothiocarbonylbenzene, triazole bactericidal agent, dispersant, wetting agent, binding agent, disintegrating agent, solid carrier mix homogeneously, after jet mill is pulverized, granulating and drying sieves and obtains the water dispersible granules of pesticidal preparations of the present invention.

Embodiment 35:21% Aminothiocarbonylbenzene diniconazole water dispersible granule (20:1)

Aminothiocarbonylbenzene 20%, hexaconazole 1%, aliphatic amine polyoxyethylene ether 4%, alkylnaphthalene sulfonate 2%, starch 3%, bentonite supplies 100%.

Embodiment 36:41% Aminothiocarbonylbenzene diniconazole water dispersible granule (1:40)

Aminothiocarbonylbenzene 1, hexaconazole 40%, polycarboxylate 2%, sodium lignin sulfonate 3%, starch 3%, Kaolin supplies 100%.

Embodiment 37:80% Aminothiocarbonylbenzene diniconazole water dispersible granule (1:3)

Aminothiocarbonylbenzene 20, hexaconazole 60%, polycarboxylate 2.5%, sodium lignin sulfonate 2%, methylcellulose 2%, white carbon supplies 100%.

Embodiment 38:51% Aminothiocarbonylbenzene Flutriafol water dispersible granules (50:1)

Aminothiocarbonylbenzene 50%, Flutriafol 1%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 2%, sodium lauryl sulphate 3%, starch 3%, Kaolin supplies 100%.

Embodiment 39:21% Aminothiocarbonylbenzene Flutriafol water dispersible granules (20:1)

Aminothiocarbonylbenzene 20%, Flutriafol 1%, sodium lignin sulfonate 3%, sodium lauryl sulphate 2%, ethyl cellulose 2%, kieselguhr supplies 100%.

Embodiment 40:20.5% Aminothiocarbonylbenzene tebuconazole water dispersible granule (40:1)

By Aminothiocarbonylbenzene 20%, Tebuconazole 0.5%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 2%, alkylbenzenesulfonate 2%, starch 2%, Kaolin supplies 100%.

Embodiment 41:42% Aminothiocarbonylbenzene epoxiconazole water dispersant (1:20)

Aminothiocarbonylbenzene 2%, epoxiconazole 40%, sodium lignin sulfonate 2.5%, alkylbenzenesulfonate 3%, starch 2%, ammonium sulfate 2%, bentonite supplies 100%.

Embodiment 42:12% Aminothiocarbonylbenzene epoxiconazole water dispersant (5:1)

Aminothiocarbonylbenzene 10%, epoxiconazole 2%, NNO1%, sodium lignin sulfonate 2%, alkylbenzenesulfonate 3%, polyvinyl alcohol 2%, sodium carbonate 2%, Kaolin supplies 100%.

Embodiment 43:33% Aminothiocarbonylbenzene difenoconazole water dispersible granule (1:10)

Aminothiocarbonylbenzene 3%, Difenoconazole 30%, NNO2%, alkylbenzenesulfonate 1.5%, methylcellulose 2%, sodium sulfate 2%, Kaolin supplies 100%.

Embodiment 44:11% Aminothiocarbonylbenzene difenoconazole water dispersible granule (10:1)

Aminothiocarbonylbenzene 10%, Difenoconazole 1%, NNO2%, alkylbenzenesulfonate 1.5%, starch 2%, sodium sulfate 2%, Kaolin supplies 100%.

Six, the preparation of suspending agent

Preparation technology: antifreezing agent, thickening agent and water are mixed, through high speed shear mix homogeneously, is sequentially added into Aminothiocarbonylbenzene, triazole bactericidal agent and surfactant, abrading-ball 2～3 hours in ball crusher, prepare the suspending agent of pesticidal preparations of the present invention.

Embodiment 458.8% Aminothiocarbonylbenzene hexaconazole suspending agent (10:1)

Aminothiocarbonylbenzene 8%, hexaconazole 0.8%, aluminium-magnesium silicate 0.3%, NNO1%, styrylphenol polyoxyethylene ether 3.5%, calcium dodecyl benzene sulfonate 1%, glycerol 1.5%, water supplies 100%.

Embodiment 465.5% Aminothiocarbonylbenzene hexaconazole suspending agent (1:10)

Aminothiocarbonylbenzene 0.5%, hexaconazole 5%, xanthan gum 0.2%, styrylphenol polyoxyethylene ether 3.5%, calcium dodecyl benzene sulfonate 1%, glycerol 1.5%, water supplies 100%.

Embodiment 478.2% Aminothiocarbonylbenzene Flutriafol suspending agent (1:40)

Aminothiocarbonylbenzene 0.2%, Flutriafol 8%, xanthan gum 0.2%, styrylphenol polyoxyethylene ether 2.5%, fatty alcohol-polyoxyethylene ether 1.5%, sodium lignin sulfonate 1%, carbamide 1.5%, water supplies 100%.

Embodiment 4810.5% Aminothiocarbonylbenzene Flutriafol suspending agent (20:1)

Aminothiocarbonylbenzene 10%, Flutriafol 0.5%, arabic gum 0.2%, styrylphenol polyoxyethylene ether 2.5%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 1.5%, sodium lignin sulfonate 1%, glycerol 1.5%, water supplies 100%.

CK19:10% Aminothiocarbonylbenzene suspending agent

Aminothiocarbonylbenzene 10%, arabic gum 0.2%, styrylphenol polyoxyethylene ether 2.5%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 1.5%, sodium lignin sulfonate 1%, glycerol 1.5%, water supplies 100%.

Embodiment 496.3% Aminothiocarbonylbenzene tebuconazole suspension concentrates (20:1)

Aminothiocarbonylbenzene 6%, Tebuconazole 0.3%, xanthan gum 0.5%, styrylphenol polyoxyethylene ether 3%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 2%, calcium dodecyl benzene sulfonate 0.5%, ethylene glycol 1.5%, water supplies 100%.

Embodiment 5020.2% Aminothiocarbonylbenzene tebuconazole suspension concentrates (1:100)

Aminothiocarbonylbenzene 0.2%, Tebuconazole 20%, xanthan gum 0.5%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 4%, calcium dodecyl benzene sulfonate 1.5%, isopropanol 2%, water supplies 100%.

Embodiment 516.6% Aminothiocarbonylbenzene difenoconazole suspending agent (10:1)

Aminothiocarbonylbenzene 6%, Difenoconazole 0.6%, aluminium-magnesium silicate 0.5%, NPE 2%, sodium lignin sulfonate 2%, calcium dodecyl benzene sulfonate 1.5%, glycerol 1%, water supplies 100%.

Embodiment 5212.3% Aminothiocarbonylbenzene difenoconazole suspending agent (1:40)

Aminothiocarbonylbenzene 0.3%, Difenoconazole 12%, aluminium-magnesium silicate 0.5%, styrylphenol polyoxyethylene ether 1%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 3%, calcium dodecyl benzene sulfonate 1.5%, carbamide 1%, water supplies 100%.

Embodiment 535.5% Aminothiocarbonylbenzene epoxiconazole suspension concentrate (10:1)

Aminothiocarbonylbenzene 5%, epoxiconazole 0.5%, xanthan gum 0.4%, NPE 1%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 3%, calcium dodecyl benzene sulfonate 1.5%, glycerol 1%, water supplies 100%.

Embodiment 5420.2% Aminothiocarbonylbenzene epoxiconazole suspension concentrate (100:1)

Aminothiocarbonylbenzene 20%, epoxiconazole 0.2%, aluminium-magnesium silicate 0.5%, di-2-ethylhexylphosphine oxide methyl naphthalene sulfonic acid 1%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 2%, styrylphenol polyoxyethylene ether 3%, ethylene glycol 1%, water supplies 100%.

Field efficacy embodiment:

In order to clear and definite Aminothiocarbonylbenzene and triazole bactericidal agent are alone with prevention effect to rice sheath blight disease and Caulis Sacchari sinensis aphid time mixed, and can the result (i.e. potentiation) of clear and definite Toxicity Determination be confirmed in field, present invention applicant different regions at home have carried out substantial amounts of field test.Aminothiocarbonylbenzene is called for short sulfo-in following complex preparation.

Field efficacy embodiment 1:

Prevent and treat wheat rust test method: with reference to pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating Cereal Rust (leaf rust, bar rust, stem rust), adopt broadcast application dispenser when sowing whole ground.

Preventing and treating wheat midge test method: with reference to pesticide field efficacy medicine test criterion (two) the 78th part insecticide control wheat midge, adopts broadcast application dispenser when sowing whole ground.

Comparison medicament: for CK1-CK6, be self-control.

Table 10 Aminothiocarbonylbenzene and the composite result of the test preventing and treating wheat rust and wheat midge of triazole bactericidal agent

It is applied to prevent and treat wheat rust, Aminothiocarbonylbenzene and composite embodiment 1,2,3 (effective ingredient dosage the is 135 grams/ha) preventive effect of hexaconazole are higher than comparison single dose 5% Aminothiocarbonylbenzene granule (formulation rate is 450 grams/ha) more than 82.61%, and preventive effect is higher than 1% hexaconazole granule (formulation rate is 225 grams/ha) more than 10.69%.It is applied to preventing and treating wheat midge, Aminothiocarbonylbenzene and composite embodiment 1,2,3 (effective ingredient dosage the is 135 grams/ha) preventive effect of hexaconazole are higher than comparison single dose 5% Aminothiocarbonylbenzene granule (formulation rate is 450 grams/ha) more than 9.78%, and preventive effect is higher than 1% hexaconazole granule (formulation rate is 225 grams/ha) more than 83.43%.Equally, when preventing and treating wheat rust and wheat midge, in effective ingredient dosage than in the less situation of single dose, the prevention effect of embodiments of the invention 4～9 is higher than each comparison single dose.Show that composition pesticide provided by the invention and preparation thereof have potentiation relative to single dose.

Field efficacy embodiment 2:

Prevent and treat wheat rust test method: with reference to pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating Cereal Rust (leaf rust, bar rust, stem rust), adopt dressing methods dispenser.

Preventing and treating wheat midge test method: with reference to pesticide field efficacy medicine test criterion (two) the 78th part insecticide control wheat midge, adopts dressing methods dispenser.

Comparison medicament: CK7-CK12 is self-control.

Table 11 Aminothiocarbonylbenzene and the composite result of the test preventing and treating wheat rust and wheat midge of triazole bactericidal agent

Be can be seen that by result of the test table 11, in effective ingredient dosage relative under the less premise of single dose, the preventive effect of the composition pesticide pesticide-germicide that the embodiment of the present invention 10～19 provides is higher than each single dose, it was shown that composition pesticide provided by the invention and preparation thereof have potentiation relative to single dose.

Field efficacy embodiment 3:

Prevent and treat wheat rust test method: with reference to pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating Cereal Rust (leaf rust, bar rust, stem rust).The test method of preventing and treating wheat midge and computational methods: with reference to GB/T24501.2-2009 stripe rust of wheat, midge worm Prevention Technique specification part 2: wheat midge.

Application method: adopt ultra-low volume spray.Comparison medicament: CK13-CK18 is self-control.

Table 12 Aminothiocarbonylbenzene and the composite result of the test preventing and treating wheat rust and wheat midge of triazole bactericidal agent

Be can be seen that by result of the test table 12, in effective ingredient dosage relative under the less premise of single dose, the preventive effect of the composition pesticide pesticide-germicide that the embodiment of the present invention 20～26 provides is higher than each single dose, it was shown that composition pesticide provided by the invention and preparation thereof have potentiation relative to single dose.

Field efficacy embodiment 4:

Prevent and treat wheat rust test method: with reference to pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating Cereal Rust (leaf rust, bar rust, stem rust).

The test method of preventing and treating wheat midge and computational methods: with reference to GB/T24501.2-2009 stripe rust of wheat, midge worm Prevention Technique specification part 2: wheat midge.

Application method: conventional spraying.

Comparison medicament: CK19:10% Aminothiocarbonylbenzene suspending agent, self-control.CK20:30% diniconazole water dispersible granule, registration card: PD20132419, Shanxi Thompson Biological Technology Co., Ltd., commercial.CK21:12.5% Flutriafol suspending agent, registration card number: PD20130373, Fengdeng Pesticide Co., Ltd., Jiangsu, commercial.CK22:430 g/l of tebuconazole suspension concentrates, registration card: PD20050216, Bayer Bitterfeld GmbH crop science company, commercial.CK23:10% difenoconazole water dispersible granule, registration card: PD20131284, Shaanxi Meibang Pesticide Co., Ltd., commercial.CK24:30% epoxiconazole suspension concentrate, registration card: PD20131832, Shaanxi Meibang Pesticide Co., Ltd., commercial.Test result is in Table 13:

Table 13 Aminothiocarbonylbenzene and the composite field control effectiveness test result preventing and treating wheat rust and wheat midge of triazole bactericidal agent

Be can be seen that by result of the test table 13, test is under the identical premise of effective ingredient dosage, composition pesticide and the preventive effect of preparation as insecticiding sterilization thereof that the embodiment of the present invention 27～53 provides are higher than each single dose, show that Pesticidal combination provided by the invention and preparation thereof have potentiation relative to single dose, it is possible to increase the preventive effect to wheat rust, midge insect pest worm.And above-mentioned composition pesticide and preparation prevention effect of 14d after medicine thereof remain to reach more than 82%, the composition pesticide lasting period of the present invention is long.

Claims (7)

1. containing the composition pesticide of Aminothiocarbonylbenzene and triazole bactericidal agent; it is characterized in that: described composition pesticide is made up of Aminothiocarbonylbenzene and triazole bactericidal agent; described Aminothiocarbonylbenzene is the bromo-N-of 3-[4-chloro-2-methyl-6-(methylamino thioformyl) benzene]-1-(3-chloropyridine-2-base)-1H-pyrazoles-5-Methanamide, and the weight ratio of described Aminothiocarbonylbenzene and triazole bactericidal agent is 100:1～1:100；Described triazole bactericidal agent is epoxiconazole.

2. the composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent according to claim 1, it is characterised in that: the weight ratio of described Aminothiocarbonylbenzene and triazole bactericidal agent is 40:1～1:40.

3. the composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent according to claim 2, it is characterised in that: the weight ratio of described Aminothiocarbonylbenzene and triazole bactericidal agent is 20:1～1:20.

4. the pesticidal preparations preventing and treating agricultural pest, it is characterized in that: the composition pesticide containing Aminothiocarbonylbenzene and triazole bactericidal agent according to any one of claim 1-3 and adjuvant form, wherein, described composition pesticide accounts for the 0.1%～80% of described pesticidal preparations gross weight.

5. a kind of pesticidal preparations preventing and treating agricultural pest according to claim 4, it is characterised in that: described composition pesticide accounts for the 1%～50% of described pesticidal preparations gross weight.

6. a kind of pesticidal preparations preventing and treating agricultural pest according to claim 4 or 5, it is characterised in that: the dosage form of described pesticidal preparations is granule, seed treatment formulations, ultra low volume liquids, water dispersible granules, wettable powder or suspending agent.

7. a kind of according to any one of claim 4-6 prevents and treats the application in controlling crop diseases and insect pests of the pesticidal preparations of agricultural pest.