CN104127192B - A kind of flow cytometer fluid system and FCM analysis method - Google Patents
A kind of flow cytometer fluid system and FCM analysis method Download PDFInfo
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- CN104127192B CN104127192B CN201310485166.2A CN201310485166A CN104127192B CN 104127192 B CN104127192 B CN 104127192B CN 201310485166 A CN201310485166 A CN 201310485166A CN 104127192 B CN104127192 B CN 104127192B
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Abstract
The open a kind of flow cytometer fluid system of the present invention and FCM analysis method, described flow cytometer fluid system includes sheath multiple abscess emitter, sample multiple abscess emitter, injector for collecting blood, hemolytic agent syringe, blood taking needle, reaction tank, flow chamber, diluent bucket, hemolytic agent bottle, the first motor, the second motor, the 3rd motor, described sheath multiple abscess emitter is driven by the first motor, described hemolytic agent syringe is driven by the 3rd motor, and described sample multiple abscess emitter and injector for collecting blood are driven by the second motor.The driving motor of sample multiple abscess emitter and injector for collecting blood is merged into one by the present invention, these two syringes use equalising torque to arrange simultaneously, motor is arranged between these two syringes, not only reduce system cost, also add integral rigidity and the inertia of system, make syringe push away sample stream more steady, improve detection accuracy.
Description
Technical field
The present invention relates to field of biological medicine, particularly relate to a kind of flow cytometer fluid system and streaming is thin
Born of the same parents' detection method.
Background technology
Flow cytometer is to control cell one by one by the hemanalysis instrument of detection zone, stream by flow cytometry
Formula cell art be phalangeal cell stream (also referred to as sample stream) under the parcel of sheath fluid, cell queue up examined by flow chamber
Surveying the fluid control technology in district, this technology can avoid cell to cause detection through detection zone by irregular route
The problem of misalignment, can greatly reduce cell overlap produces the probability of missing inspection by detection zone simultaneously.
Existing flow cytometry is broadly divided into two big classes, and the first kind is sheath stream and sample stream all pushes away sample with syringe,
Equations of The Second Kind is that sample stream uses syringe to push away sample, and sheath stream uses constant voltage source of the gas to carry out pushing away sample.Constant voltage source of the gas drives sheath
Stream needs a set of constant voltage air-channel system in addition, and cost is the highest.
Fluid scheme such as Fig. 1 institute of first kind flow cytometry (i.e. sheath stream and sample stream all pushes away sample with syringe)
Showing, this fluid system is by sheath multiple abscess emitter 5 ', sample multiple abscess emitter 13 ', injector for collecting blood 17 ', hemolytic agent note
Emitter 22 ', blood taking needle 19 ', reaction tank 20 ', flow chamber 10 ', diluent bucket 1 ', hemolytic agent bottle 24 ', three
Logical valve 2 ', two-way valve 3 ', two-way valve 8 ', two-way valve 11 ', two-way valve 18 ', three-way valve 23 ', three way cock
6 ', three way cock 7 ', three way cock 9 ', three way cock 14 ', three way cock 15 ', diluent entrance 30 ',
Flow chamber waste liquid outlet 31 ' and the connected pipeline composition used of each device.Its mesotheca multiple abscess emitter 5 ' is by motor 4 '
Drive, sample multiple abscess emitter 13 ' is driven by motor 12 ', injector for collecting blood 17 ' is driven by motor 16 ', hemolytic agent
Syringe 22 ' is driven by motor 21 '.
The workflow of first kind flow cytometer is as follows:
1) taken a blood sample by injector for collecting blood 17 ' and blood taking needle 19 '；
2) rear blood taking needle 19 ' of having taken a blood sample enters reaction tank 20 ', then separates specified blood sample and enters reaction tank 20；
3) add hemolytic agent entrance reaction tank 20 by hemolytic agent syringe 22 ' and three-way valve 23 ' to carry out instead with blood sample
Should；
4) after blood sample has reacted with hemolytic agent, by sheath multiple abscess emitter 5 ' and two-way valve 8 ' (closing three-way valve 2 ')
By blood sample sucking-off reaction tank；
5) sheath multiple abscess emitter 5 pushes away sample together with sample multiple abscess emitter 13 ', is first turned on two-way valve 11 ' and threeway
Valve 2 ', cuts out two-way valve 18 ', and sheath multiple abscess emitter 5 ' can assist and push away blood sample and rapidly enter flow chamber 10 ', then blood
Sample is pushed the detection zone of flow chamber 10 ' again by sample multiple abscess emitter 13 ', now opens three-way valve 2 ' and two-way valve 3 ',
Diluent is pushed flow chamber 10 ' by sheath multiple abscess emitter 5 ' and forms sheath stream, thus wraps up blood sample and queue up by flowing
Room 10 ' carries out hemocyte detection.
So, each syringe of first kind flow cytometer needs individually to join a motor and is driven, due to
The holistic cost of motor and supporting driving actuating device is higher, the therefore high expensive of the program.And, stream
Formula cell instrument is high to the stability requirement of sample stream, and therefore rigidity and inertia to syringe require height, material
Batch difficult quality controls.
Equations of The Second Kind flow cytometer is then owing to add a set of constant voltage air-channel system, cost intensive, and simultaneity factor is multiple
Miscellaneous degree height, inconvenience is safeguarded.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of flow cytometer fluid system
And FCM analysis method, it is intended to the cost solving existing flow cytometer is high, and inconvenience is safeguarded, sample fluid capacitance
The problems such as susceptible.
Technical scheme is as follows:
A kind of flow cytometer fluid system, including sheath multiple abscess emitter, sample multiple abscess emitter, injector for collecting blood,
Hemolytic agent syringe, blood taking needle, reaction tank, flow chamber, diluent bucket, hemolytic agent bottle, the first motor,
Two motors, the 3rd motor, the first three-way valve, the first two-way valve, the second two-way valve, the 4th two-way valve, second
Three-way valve；Sheath stream input port, sample stream input port and flow chamber waste liquid outlet it is provided with on described flow chamber；Sheath
The dispensing end of multiple abscess emitter is connected with described sheath stream input port and described sample stream input port respectively；Described sheath multiple abscess
Emitter is divided and is arranged described first three-way valve on the pipeline between described sheath stream input port, described first three-way valve
One end is connected with described diluent bucket；Arrange on pipeline between described first three-way valve and described sheath stream input port
Described first two-way valve；Described is arranged on pipeline between described sheath multiple abscess emitter and described sample stream input port
Two two-way valves；The dispensing end of sample multiple abscess emitter is connected with described sample stream input port and reative cell respectively；Described
The connection of sample stream input port is from the pipeline of described sample multiple abscess emitter Yu described sheath multiple abscess emitter；Described sample stream
Described 4th two-way valve it is provided with reative cell on the pipeline that syringe is connected；Described injector for collecting blood and blood taking needle
Being connected, described diluent entrance is arranged on described injector for collecting blood；The dispensing end of hemolytic agent syringe arranges institute
State the second three-way valve, be connected with reaction tank and hemolytic agent bottle respectively；Described sheath multiple abscess emitter is driven by the first motor,
Described hemolytic agent syringe is driven by the 3rd motor, wherein, described sample multiple abscess emitter and injector for collecting blood by
Second motor drives.
Described flow cytometer fluid system, wherein, sample multiple abscess is penetrated and is used equalising torque with injector for collecting blood
Arrange, arrange that distance meets formula L1 × F1=L2 × F2；Wherein, L1 is sample multiple abscess emitter and the second motor
The distance at center, L2 is the distance at the center of injector for collecting blood and the second motor, and F1 is sample multiple abscess emitter
Promotion resistance, F2 is the promotion resistance of injector for collecting blood.
Described flow cytometer fluid system, wherein, on the pipeline being connected between injector for collecting blood with blood taking needle
Being provided with the 3rd three-way valve, one end of the 3rd three-way valve is cleaned entrance with sampling needle and is connected.
Described flow cytometer fluid system, wherein, the dispensing end of sample multiple abscess emitter is defeated with described sample stream
Entrance is connected on pipeline and is provided with a bypass, described bypass and described sheath multiple abscess emitter and described sample stream input port
The pipeline being connected is connected, and described bypass arranges the 3rd two-way valve.
Described flow cytometer fluid system, wherein, described flow cytometer fluid system also includes the second
Hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve；
Described the second hemolytic agent syringe is driven by the 3rd motor, and the dispensing end of the second hemolytic agent syringe sets
Put described 4th three-way valve, be connected with reaction tank and the second hemolytic agent bottle respectively.
A kind of FCM analysis method, wherein, described FCM analysis method uses streaming as above
Cell instrument fluid system is carried out, and specifically includes following steps:
1) connecting injector for collecting blood and the pipeline of blood taking needle by the 3rd three-way valve, the second motor drives blood sampling injection
Device, is taken a blood sample by blood taking needle；Open the second two-way valve and the first three-way valve simultaneously, sample multiple abscess emitter from
Diluent drawn by diluent bucket；
2) rear blood taking needle of having taken a blood sample enters reaction tank, then separates specified blood sample and enters reaction tank；
3) the 3rd motor drives hemolytic agent syringe, draws hemolytic agent, then by described the from hemolytic agent bottle
The pipeline of hemolytic agent syringe and reaction tank connected by two three-way valve, adds to hemolytic agent in reaction tank to enter with blood sample
Row reaction；
4), after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve,
First motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank；
5) the second motor drives sample multiple abscess emitter, directly pushes away blood sample and pushes the detection zone of flow chamber；Open first
Three-way valve and the first two-way valve, the first motor drives sheath multiple abscess emitter, and diluent is pushed flowing by sheath multiple abscess emitter
Room and form sheath stream, thus wrap up blood sample queue up carry out blood cell analysis detection by flow chamber.
A kind of FCM analysis method, wherein, described FCM analysis method uses streaming as above
Cell instrument fluid system is carried out, and specifically includes following steps:
1) connecting injector for collecting blood and the pipeline of blood taking needle by the 3rd three-way valve, the second motor drives blood sampling injection
Device, is taken a blood sample by blood taking needle；Open the 3rd two-way valve and the first three-way valve simultaneously, sample multiple abscess emitter from
Diluent drawn by diluent bucket；
2), after having taken a blood sample, blood taking needle enters reaction tank, separates specified blood sample and enters reaction tank；
3) the 3rd motor drives hemolytic agent syringe, draws hemolytic agent, then by described the from hemolytic agent bottle
The pipeline of hemolytic agent syringe and reaction tank connected by two three-way valve, adds to hemolytic agent in reaction tank to enter with blood sample
Row reaction；
4), after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve,
First motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank；
5) opening the 3rd two-way valve and the first three-way valve, close the 4th two-way valve, the first motor drives sheath multiple abscess to penetrate
Device, the second motor drives sample multiple abscess emitter, and blood sample is pushed flow chamber by sheath multiple abscess emitter and sample multiple abscess emitter,
Then blood sample is pushed the detection zone of flow chamber 1 again by sample multiple abscess emitter；Open the first three-way valve and first liang to lead to
Valve, the first motor drives sheath multiple abscess emitter, and diluent is pushed flow chamber by sheath multiple abscess emitter and forms sheath stream, from
And wrap up blood sample queuing and carry out blood cell analysis detection by flow chamber.
Described FCM analysis method, wherein, step 5) in, when the second motor action, sampling note
Emitter links together with sample multiple abscess emitter, and sampling syringe connected by the 3rd three-way valve and sampling needle cleans entrance
Pipeline, sampling syringe pushes away diluent to sampling needle and cleans entrance to clean sampling needle.
Described FCM analysis method, wherein, described flow cytometer fluid system also includes that the second is molten
Blood agent syringe, the second hemolytic agent bottle and the 4th three-way valve；Described the second hemolytic agent syringe is by the 3rd electricity
Machine drives, and the dispensing end of the second hemolytic agent syringe arranges described 4th three-way valve, respectively with reaction tank and the
Two kinds of hemolytic agent bottles are connected；Complete step 3) after, further comprising the steps of:
3rd motor drives the second hemolytic agent syringe, draws the second hemolytic agent from the second hemolytic agent bottle,
Then the pipeline of the second hemolytic agent syringe and reaction tank is connected by described 4th three-way valve, the second is molten
Blood agent is added in reaction tank and is reacted with blood sample.
Flow cytometer fluid system provided by the present invention, by sample multiple abscess emitter and the driving of injector for collecting blood
Motor merges into one, and these two syringes use equalising torque to arrange simultaneously, and motor is arranged in these two injections
Between device, it has the beneficial effects that: 1) reduce system cost；2) after two syringe common motor, overall firm
Property and inertia increase, it is more steady that syringe pushes away sample stream, improves detection accuracy；3) two syringe equalising torque
Arrange that rear drive resistance can be cancelled out each other, driving motor and the service life of syringe can be improved.
Accompanying drawing explanation
Fig. 1 is the structural representation of first kind flow cytometer in prior art.
Fig. 2 is the structural representation of flow cytometer fluid system provided by the present invention.
Fig. 3 is the structural representation that in the present invention, sample multiple abscess emitter is arranged with injector for collecting blood equalising torque.
Fig. 4 is the structural representation of flow cytometer fluid system in the embodiment of the present invention 1.
Fig. 5 is the structural representation of flow cytometer fluid system in the embodiment of the present invention 2.
Fig. 6 is the structural representation of flow cytometer fluid system in the embodiment of the present invention 3.
Detailed description of the invention
The present invention provides a kind of flow cytometer fluid system and FCM analysis method, for making the mesh of the present invention
, technical scheme and effect clearer, clear and definite, the present invention is described in more detail below.Should be appreciated that
Specific embodiment described herein only in order to explain the present invention, is not intended to limit the present invention.
A kind of flow cytometer fluid system provided by the present invention, it is mainly improved by, by by sample stream
The driving motor of syringe and injector for collecting blood merges into one, decreases a set of motor and drives actuating device,
Cost can be reduced, and the stationarity of sample stream can be improved.Simultaneously because just for sample multiple abscess emitter and adopting
Blood syringe merges driving motor, and (this two syringes action during flow cytometry analysis is few, can be wrong
Begin to rehearse cloth syringe action), therefore overall measurement speed is unaffected.
Specifically, described flow cytometer fluid system, as in figure 2 it is shown, include sheath multiple abscess emitter 5, sample
Multiple abscess emitter 13, injector for collecting blood 17, hemolytic agent syringe 22, blood taking needle 19, reaction tank 20, flow chamber
10, diluent bucket 1, hemolytic agent bottle the 24, first motor the 4, second motor the 12, the 3rd motor the 21, the 1st
Logical valve the 2, first two-way valve the 3, second two-way valve the 8, the 3rd two-way valve the 11, the 4th two-way valve the 18, the 2nd 3
Logical valve the 23, the 3rd three-way valve the 25, first three way cock the 6, second three way cock the 7, the 3rd three way cock 9,
4th three way cock the 14, the 5th three way cock 15 and the connected pipeline used of each device；Described 1st connects
The 6, second three way cock the 7, the 3rd three way cock the 9, the 4th three way cock the 14, the 5th three way cock 15 uses
In making pipeline generate bypass, corresponding parts are made to be connected；
Described sheath multiple abscess emitter 5 is connected with described first motor 4, the first motor 4 drive；Described sample stream
Syringe 13 is connected with the second motor 12 respectively with injector for collecting blood 17, is driven by the second motor 12；Institute
State hemolytic agent syringe 22 to be connected with the 3rd motor 21, the 3rd motor 21 drive；
Sheath stream input port, sample stream input port and flow chamber waste liquid outlet 31 it is provided with on described flow chamber 10；
The dispensing end of described sheath multiple abscess emitter 5 is divided into twice pipeline through described first three way cock 6, respectively with
The sheath stream input port of flow chamber 10 is connected with the sample stream input port of flow chamber 10；Described sheath multiple abscess emitter 5 with
Described first three-way valve 2, described first three-way valve 2 are set on the pipeline between the sheath stream input port of flow chamber 10
One end (normally opened end) be connected with described diluent bucket 1；Described first three-way valve 2 and the sheath stream of flow chamber 10
Described first two-way valve 3 is also set up on pipeline between input port；Described sheath multiple abscess emitter 5 and flow chamber 10
Described second two-way valve 8 is set on the pipeline between sample stream input port；
The dispensing end of described sample multiple abscess emitter 13 is divided into twice pipeline through described 5th three way cock 15, point
It is not connected with sample stream input port and the reative cell 20 of described flow chamber 10；The sample stream of described flow chamber 10 is defeated
Porch arranges described 3rd three way cock 9, and connection is penetrated from described sample multiple abscess emitter 13 with described sheath multiple abscess
The pipeline of device 5；Described 4th liang it is provided with on the pipeline that described sample multiple abscess emitter 13 is connected with reative cell 20
Logical valve 18；
Described injector for collecting blood 17 is connected with blood taking needle 19, and described diluent entrance 30 is arranged on described blood sampling note
In emitter 17；
The dispensing end of described hemolytic agent syringe 22 arranges described second three-way valve 23, respectively with reaction tank 20 and
Hemolytic agent bottle 24 is connected (the normally opened end of described second three-way valve 23 is connected) with described solvent blood vial 24.
As it is shown on figure 3, sample multiple abscess emitter 13 and injector for collecting blood 17 are carried out equalising torque layout, sample
The distance at the center of multiple abscess emitter 13 and the second motor 12 is L1, injector for collecting blood 17 and the second motor 12
The distance at center is L2, and the promotion resistance of sample multiple abscess emitter 13 is F1, and the promotion resistance of syringe 17 is
F2, layout distance L1 of syringe and L2 meet formula: L1 × F1=L2 × F2, i.e. meet equalising torque, because of
This can improve driving stationarity and motor service life.Simultaneously because the existence of two syringes, integral rigidity
And inertia increases, motor-driven gap injustice stability can be buffered, it is thus achieved that ratio drives single syringe scheme more
Sample stream smoothly, thus improve the accuracy of detection of fluidic cell.
In the present invention, in order to reduce cost, sample multiple abscess emitter 13 and injector for collecting blood 17 share an electricity
Machine, affects the blood sample in blood taking needle 19 when of in order to avoid sample multiple abscess emitter 13 action, described blood sampling is noted
Described 3rd three-way valve 25 can be set on the pipeline that emitter 17 is connected with blood taking needle 19.Described 3rd three-way valve 25
Normally opened end connect sampling needle clean entrance 29, the most only needs by injector for collecting blood 17 carry out blood sampling and
Just opening described 3rd three-way valve 25 when of dividing blood, the 3rd three-way valve 25 described in other moment is in closedown shape
State, connects described injector for collecting blood 17 and sampling needle cleans entrance 29.When the second motor 12 action, close
3rd three-way valve 25, to blood in blood taking needle 19 when just thus can eliminate sample multiple abscess emitter 13 action
Sample produces impact.
Being connected between dispensing end and the sample stream input port of described flow chamber 10 of described sample multiple abscess emitter 13
The sample of a bypass, described bypass and described sheath multiple abscess emitter 5 and described flow chamber 10 it is also provided with on pipeline
The pipeline that this stream input port is connected is connected, and described bypass arranges described 3rd two-way valve 11.Specifically, be
Described 4th three way cock 14 on pipeline between described sample multiple abscess emitter 13 and described 5th three way cock 15,
Generating described bypass, accordingly, described sheath multiple abscess emitter 5 is connected with the sample stream input port of described flow chamber 10
Pipeline on be correspondingly arranged on the second three way cock 7, be connected with described bypass.So, when sample multiple abscess emitter
When 13 promotion blood samples enter flow chamber 10, opening described second two-way valve 11, described sheath multiple abscess emitter 5 just may be used
To assist, blood sample is quickly pushed flow chamber 10.
Also providing for a kind of FCM analysis method in the present invention, described method is to use described flow cytometer stream
System system is carried out, and its workflow is as follows:
1) opening the 3rd three-way valve 25, the second motor 12 is driven injector for collecting blood 17, is entered by blood taking needle 19
Row blood sampling；Opening the 3rd two-way valve 11 and the first three-way valve 2, sample multiple abscess emitter 13 is from diluent bucket 1 simultaneously
Draw diluent；(open the 3rd three-way valve the 25, the 3rd two-way valve 11 and the first three-way valve 2 simultaneously, make sample
Multiple abscess emitter 13 and injector for collecting blood 17 work simultaneously, to sample multiple abscess when can eliminate the second motor 12 action
The impact of liquid in emitter 13 and reaction tank 20)；
2) after having taken a blood sample, blood taking needle 19 enters reaction tank 20, separates specified blood sample and enters reaction tank 20；
3) the 3rd motor 21 drives hemolytic agent syringe 22, draws hemolytic agent, then from hemolytic agent bottle 24
Open described second three-way valve 23, hemolytic agent is added in reaction tank 20 and react with blood sample；
4), after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close first
Three-way valve 2, the first motor 4 drives sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20；
5) opening the 3rd two-way valve 11 and the first three-way valve 2, close the 4th two-way valve 18, the first motor 4 drives
Dynamic sheath multiple abscess emitter 5, the second motor 12 drives sample multiple abscess emitter 13, sheath multiple abscess emitter 5 and sample multiple abscess to penetrate
Blood sample is pushed flow chamber 10 by device 13, and then blood sample is pushed the inspection of flow chamber 10 again by sample multiple abscess emitter 13
Survey district；Opening the first three-way valve 2 and the first two-way valve 3, the first motor 4 drives sheath multiple abscess emitter 5, diluent
Pushed flow chamber 10 by sheath multiple abscess emitter 5 and form sheath stream, thus wrap up blood sample and queue up to be entered by flow chamber 10
Promoting the circulation of blood cell analysis detects.
Wherein, step 5) in, when the second motor 12 action, sampling syringe 17 and sample multiple abscess emitter
13 link together, and the 3rd three-way valve 25 is closed, and it is clear to sampling needle that sampling syringe 17 pushes away diluent
Wash mouth 29 to clean sampling needle.
Owing to reducing by a motor, the driving actuating device of motor also reduces a set of simultaneously, and increases a threeway
The cost that valve brings is far below the cost of a set of motor, and technical scheme cost the most provided by the present invention drops significantly
Low.Meanwhile, sample multiple abscess emitter and injector for collecting blood use equalising torque to arrange, integral rigidity and inertia increase
Adding, available buffer drives the gap injustice stability of motor, can obtain sample stream more stably, thus improve streaming
The accuracy of detection of cell instrument.It addition, after sample multiple abscess emitter and injector for collecting blood merge into the driving of same motor,
Two motor action times were not staggered arrangement, not and affect the measuring speed of flow cytometer.
The present invention will be further described by the following examples.
Embodiment 1:
As shown in Figure 4, the composition of a kind of flow cytometer fluid system includes sheath multiple abscess emitter 5, sample multiple abscess
Emitter 13, injector for collecting blood 17, hemolytic agent syringe 22, blood taking needle 19, reaction tank 20, flow chamber 10,
Diluent bucket 1, hemolytic agent bottle the 24, first three-way valve the 2, first two-way valve the 3, second two-way valve 8, the 4th liang
Logical valve the 18, second three-way valve the 23, the 3rd three-way valve the 25, first three way cock the 6, the 4th three way cock 14,
5th three way cock 15 and the connected pipeline used of each device.29 clean entrance for sampling needle, and 30 is diluent
Entrance, 31 is flow chamber waste liquid outlet.Wherein, sheath multiple abscess emitter 5 driven by the first motor 4, sample multiple abscess
Emitter 13 and injector for collecting blood 17 are driven by the second motor 12, hemolytic agent syringe 22 is by the 3rd motor 21
Drive.Sample multiple abscess emitter 13 and injector for collecting blood 17 use equalising torque to arrange, the second motor 12 is arranged in
Between two syringes.
The difference of the scheme in the program and Fig. 2 resides in reduced the bypass at the 3rd two-way valve 11 place, i.e. without auxiliary
Boosting sample stream branch road.
Program workflow is as follows:
1) opening the 3rd three-way valve 25, the second motor 12 is driven injector for collecting blood 17, is entered by blood taking needle 19
Row blood sampling；Opening the second two-way valve 8 and the first three-way valve 2, sample multiple abscess emitter 13 is from diluent bucket 1 simultaneously
Draw diluent；(to the shadow of liquid in sample multiple abscess emitter 13 and reaction tank 20 during elimination motor 12 action
Ring)；
2) rear blood taking needle 19 of having taken a blood sample enters reaction tank 20, then separates specified blood sample and enters reaction tank 20；
3) the 3rd motor 21 drives hemolytic agent syringe 22, draws hemolytic agent, then from hemolytic agent bottle 24
Open described second three-way valve 23, hemolytic agent is added in reaction tank 20 and react with blood sample；
4), after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close first
Three-way valve 2, the first motor 4 drives sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20；
5) the second motor 12 drives sample multiple abscess emitter 13, directly pushes away blood sample and pushes the detection zone of flow chamber 10
(the second motor 12 action, sampling syringe 17 links together with sample multiple abscess emitter 13, the 3rd three-way valve
25 do not open, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle)；Open
One three-way valve 2 and the first two-way valve 3, the first motor 4 drives sheath multiple abscess emitter 5, and diluent is by sheath multiple abscess emitter
5 push flow chamber 10 and form sheath stream, thus wrap up blood sample queuing and carry out blood cell analysis by flow chamber 10
Detection.
Embodiment 2:
As it is shown in figure 5, the composition of a kind of flow cytometer fluid system includes sheath multiple abscess emitter 5, sample multiple abscess
Emitter 13, injector for collecting blood 17, hemolytic agent syringe 22, the second hemolytic agent syringe 26, blood taking needle 19,
Reaction tank 20, flow chamber 10, diluent bucket 1, hemolytic agent bottle 24, the second hemolytic agent bottle the 28, the 1st
Logical valve the 2, first two-way valve the 3, second two-way valve the 8, the 4th two-way valve the 18, second three-way valve the 23, the 3rd 3
Logical valve the 25, the 4th three-way valve the 27, first three way cock the 6, the 4th three way cock the 14, the 5th three way cock 15
And each device is connected pipeline used.29 clean entrance for sampling needle, and 30 is diluent entrance, and 31 is flowing
Room waste liquid outlet.Wherein, sheath multiple abscess emitter 5 driven by the first motor 4, sample multiple abscess emitter 13 and blood sampling note
Emitter 17 is driven by the second motor 12, hemolytic agent syringe 22 and the second hemolytic agent syringe 26 be by the 3rd
Motor 21 drives.Sample multiple abscess emitter 13 is arranged with injector for collecting blood 17 equalising torque, the second motor 12 cloth
It is placed between two syringes.
Program workflow:
1) opening the 3rd three-way valve 25, the second motor 12 is driven injector for collecting blood 17, is entered by blood taking needle 19
Row blood sampling；Opening the second two-way valve 8 and the first three-way valve 2, sample multiple abscess emitter 13 is from diluent bucket 1 simultaneously
Draw diluent；(to the shadow of liquid in sample multiple abscess emitter 13 and reaction tank 20 during elimination motor 12 action
Ring)；
2) rear blood taking needle 19 of having taken a blood sample enters reaction tank 20, then separates specified blood sample and enters reaction tank 20；
3) the 3rd motor 21 drives hemolytic agent syringe 22, draws hemolytic agent, then from hemolytic agent bottle 24
Open described second three-way valve 23, hemolytic agent is added in reaction tank 20 and react with blood sample；
4) the 3rd motor 21 drives the second hemolytic agent syringe 26, draws from the second hemolytic agent bottle 28
The second hemolytic agent, then opens described 4th three-way valve 27, adds the second hemolytic agent to reaction tank 20
In react with blood sample；
5), after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close first
Three-way valve 2, the first motor 4 drives sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20；
6) the second motor 12 drives sample multiple abscess emitter 13, directly pushes away blood sample and pushes the detection zone of flow chamber 10
(the second motor 12 action, sampling syringe 17 links together with sample multiple abscess emitter 13, the 3rd three-way valve
25 do not open, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle)；Open
One three-way valve 2 and the first two-way valve 3, the first motor 4 drives sheath multiple abscess emitter 5, and diluent is by sheath multiple abscess emitter
5 push flow chamber 10 and form sheath stream, thus wrap up blood sample queuing and carry out blood cell analysis by flow chamber 10
Detection.
Embodiment 2 is with the difference of embodiment 1, for strengthening the reaction effect of blood sample and hemolytic agent, increases by one
Plant hemolytic agent, therefore, the present embodiment scheme adds the second hemolytic agent syringe the 26, the 4th three-way valve 27
With the second hemolytic agent bottle 28.
Embodiment 3
As shown in Figure 6, the composition of a kind of flow cytometer fluid system includes sheath multiple abscess emitter 5, sample multiple abscess
Emitter 13, injector for collecting blood 17, hemolytic agent syringe 22, the second hemolytic agent syringe 26, blood taking needle 19,
Reaction tank 20, flow chamber 10, diluent bucket 1, hemolytic agent bottle 24, the second hemolytic agent bottle the 28, the 1st
Logical valve the 2, first two-way valve the 3, second two-way valve the 8, the 3rd two-way valve the 11, the 4th two-way valve the 18, the 2nd 3
Logical valve 23, the 3rd three-way valve 25, the 4th three-way valve the 27, first three way cock the 6, second three way cock 7, the
Three three way cock the 9, the 4th three way cock the 14, the 5th three way cock 15 and the connected pipeline used of each device.29
Cleaning entrance for sampling needle, 30 is diluent entrance, and 31 is flow chamber waste liquid outlet.Wherein, wherein, sheath stream
Syringe 5 is driven by the first motor 4, sample multiple abscess emitter 13 and injector for collecting blood 17 be by the second motor 12
Driving, hemolytic agent syringe 22 and the second hemolytic agent syringe 26 are driven by the 3rd motor 21.Sample multiple abscess
Emitter 13 is arranged with injector for collecting blood 17 equalising torque, and the second motor 12 is arranged between two syringes.
Program workflow:
1) opening the 3rd three-way valve 25, the second motor 12 is driven injector for collecting blood 17, is entered by blood taking needle 19
Row blood sampling；Opening the 3rd two-way valve 11 and the first three-way valve 2, sample multiple abscess emitter 13 is from diluent bucket 1 simultaneously
Draw diluent；(to liquid in sample multiple abscess emitter 13 and reaction tank 20 when eliminating the second motor 12 action
Impact)；
2) after having taken a blood sample, blood taking needle 19 enters reaction tank 20, separates specified blood sample and enters reaction tank 20；
3) the 3rd motor 21 drives hemolytic agent syringe 22, draws hemolytic agent, then from hemolytic agent bottle 24
Open described second three-way valve 23, hemolytic agent is added in reaction tank 20 and react with blood sample；
4) the 3rd motor 21 drives the second hemolytic agent syringe 26, draws from the second hemolytic agent bottle 28
The second hemolytic agent, then opens described 4th three-way valve 27, adds the second hemolytic agent to reaction tank 20
In react with blood sample；
5), after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close first
Three-way valve 2, the first motor 4 drives sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20；
6) opening the 3rd two-way valve 11 and the first three-way valve 2, close the 4th two-way valve 18, the first motor 4 drives
Dynamic sheath multiple abscess emitter 5, the second motor 12 drives sample multiple abscess emitter 13, sheath multiple abscess emitter 5 and sample multiple abscess to penetrate
Blood sample is pushed flow chamber 10 by device 13 jointly, and then blood sample is pushed flow chamber 10 by sample multiple abscess emitter 13 again
Detection zone (the second motor 12 action, sampling syringe 17 links together with sample multiple abscess emitter 13, the 3rd
Three-way valve 25 is not opened, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle)；
Opening the first three-way valve 2 and the first two-way valve 3, the first motor 4 drives sheath multiple abscess emitter 5, and diluent is by sheath stream
Syringe 5 pushes flow chamber 10 and forms sheath stream, thus wraps up blood sample and queue up that to carry out blood by flow chamber 10 thin
Born of the same parents analyze detection.
Embodiment 3 is with the difference of embodiment 2, adds the bypass at the 3rd two-way valve 11 place, i.e. increases
Auxiliary pushes away sample stream branch road so that blood sample quickly to push flow chamber 10.
The driving motor of sample multiple abscess emitter and injector for collecting blood is all merged into one by three above embodiment, with
Time these two syringes use equalising torques to arrange, motor is arranged between these two syringes, its beneficial effect
It is: 1) reduce system cost；2), after two syringe common motor, integral rigidity and inertia increase, injection
It is more steady that device pushes away sample stream, improves detection accuracy；3) two syringe equalising torques arrange that rear drive resistance can phase
Offset mutually, driving motor and the service life of syringe can be improved.
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, those of ordinary skill in the art are come
Saying, can be improved according to the above description or convert, all these modifications and variations all should belong to institute of the present invention
Attached scope of the claims.
Claims (6)
1. a flow cytometer fluid system, including sheath multiple abscess emitter, sample multiple abscess emitter, injector for collecting blood,
Hemolytic agent syringe, blood taking needle, reaction tank, flow chamber, diluent bucket, hemolytic agent bottle, the first motor,
Two motors, the 3rd motor, the first three-way valve, the first two-way valve, the second two-way valve, the 4th two-way valve, second
Three-way valve；Sheath stream input port, sample stream input port and flow chamber waste liquid outlet it is provided with on described flow chamber；Sheath
The dispensing end of multiple abscess emitter is connected with described sheath stream input port and described sample stream input port respectively；Described sheath multiple abscess
Emitter is divided and is arranged described first three-way valve on the pipeline between described sheath stream input port, described first three-way valve
One end is connected with described diluent bucket；Arrange on pipeline between described first three-way valve and described sheath stream input port
Described first two-way valve；Described is arranged on pipeline between described sheath multiple abscess emitter and described sample stream input port
Two two-way valves；The dispensing end of sample multiple abscess emitter is connected with described sample stream input port and reative cell respectively；Described
The connection of sample stream input port is from the pipeline of described sample multiple abscess emitter Yu described sheath multiple abscess emitter；Described sample stream
Described 4th two-way valve it is provided with reative cell on the pipeline that syringe is connected；Described injector for collecting blood and blood taking needle
Being connected, described diluent entrance is arranged on described injector for collecting blood；The dispensing end of hemolytic agent syringe arranges institute
State the second three-way valve, be connected with reaction tank and hemolytic agent bottle respectively；Described sheath multiple abscess emitter is driven by the first motor,
Described hemolytic agent syringe is driven by the 3rd motor, it is characterised in that described sample multiple abscess emitter and blood sampling injection
Device is driven by the second motor；
The dispensing end of sample multiple abscess emitter is connected with described sample stream input port on pipeline and is provided with a bypass, described
The pipeline that is connected with described sample stream input port with described sheath multiple abscess emitter of bypass is connected, and described bypass arranges the
Three two-way valves；
The 3rd three-way valve, the one of the 3rd three-way valve it is provided with on the pipeline being connected between injector for collecting blood with blood taking needle
Hold and be connected with sampling needle cleaning entrance.
Flow cytometer fluid system the most according to claim 1, it is characterised in that sample multiple abscess penetrate with
Injector for collecting blood uses equalising torque to arrange, arranges that distance meets formula L1 × F1=L2 × F2；Wherein, L1
For the distance of sample multiple abscess emitter Yu the center of the second motor, L2 is the center of injector for collecting blood and the second motor
Distance, F1 is the promotion resistance of sample multiple abscess emitter, and F2 is the promotion resistance of injector for collecting blood.
Flow cytometer fluid system the most according to claim 1, it is characterised in that described fluidic cell
Instrument fluid system also includes the second hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve；
Described the second hemolytic agent syringe is driven by the 3rd motor, and the dispensing end of the second hemolytic agent syringe sets
Put described 4th three-way valve, be connected with reaction tank and the second hemolytic agent bottle respectively.
4. a FCM analysis method, it is characterised in that described FCM analysis method is to use such as power
Profit requires what the flow cytometer fluid system described in 1 was carried out, specifically includes following steps:
1) connecting injector for collecting blood and the pipeline of blood taking needle by the 3rd three-way valve, the second motor drives blood sampling injection
Device, is taken a blood sample by blood taking needle；Open the 3rd two-way valve and the first three-way valve simultaneously, sample multiple abscess emitter from
Diluent drawn by diluent bucket；
2), after having taken a blood sample, blood taking needle enters reaction tank, separates specified blood sample and enters reaction tank；
3) the 3rd motor drives hemolytic agent syringe, draws hemolytic agent, then by described the from hemolytic agent bottle
The pipeline of hemolytic agent syringe and reaction tank connected by two three-way valve, adds to hemolytic agent in reaction tank to enter with blood sample
Row reaction；
4), after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve,
First motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank；
5) opening the 3rd two-way valve and the first three-way valve, close the 4th two-way valve, the first motor drives sheath multiple abscess to penetrate
Device, the second motor drives sample multiple abscess emitter, and blood sample is pushed flow chamber by sheath multiple abscess emitter and sample multiple abscess emitter,
Then blood sample is pushed the detection zone of flow chamber again by sample multiple abscess emitter；Open the first three-way valve and the first two-way valve,
First motor drives sheath multiple abscess emitter, and diluent is pushed flow chamber by sheath multiple abscess emitter and forms sheath stream, thus wraps
Wrap up in blood sample queuing and carry out blood cell analysis detection by flow chamber.
FCM analysis method the most according to claim 4, it is characterised in that step 5) in, when
During the second motor action, sampling syringe links together with sample multiple abscess emitter, and sampling note connected by the 3rd three-way valve
Emitter and sampling needle clean the pipeline of entrance, and sampling syringe pushes away diluent and adopts to clean to sampling needle cleaning entrance
Sample pin.
FCM analysis method the most according to claim 4, it is characterised in that described flow cytometer
Fluid system also includes the second hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve；Described second
Planting hemolytic agent syringe to be driven by the 3rd motor, the dispensing end of the second hemolytic agent syringe arranges the described 4th 3
Logical valve, is connected with reaction tank and the second hemolytic agent bottle respectively；Complete step 3) after, further comprising the steps of:
3rd motor drives the second hemolytic agent syringe, draws the second hemolytic agent from the second hemolytic agent bottle,
Then the pipeline of the second hemolytic agent syringe and reaction tank is connected by described 4th three-way valve, the second is molten
Blood agent is added in reaction tank and is reacted with blood sample.
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| CN201310485166.2A CN104127192B (en) | 2013-10-16 | 2013-10-16 | A kind of flow cytometer fluid system and FCM analysis method |
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| CN104713816B (en) * | 2015-02-04 | 2017-06-23 | 深圳市帝迈生物技术有限公司 | A kind of whole blood CRP detection means and method and a kind of blood cell analyzer |
| CN104897557B (en) * | 2015-05-30 | 2018-03-09 | 广州埃克森生物科技有限公司 | FCM analysis liquid-way system and FCM analysis method |
| CN104914031A (en) * | 2015-06-12 | 2015-09-16 | 广州埃克森生物科技有限公司 | Flow cytometry fluid path system and flow cytometry method |
| CN104914263B (en) * | 2015-06-24 | 2017-05-31 | 广州埃克森生物科技有限公司 | Blood cell analyzer strength cleaning solution automatic filling method and device |
| CN105717034A (en) * | 2016-02-01 | 2016-06-29 | 深圳开立生物医疗科技股份有限公司 | Liquid path system and flow cytometry detection method thereof |
| CN105784571B (en) * | 2016-02-29 | 2023-05-26 | 深圳市帝迈生物技术有限公司 | Double-pool measuring method and device for specific reaction protein CRP |
| CN105675897A (en) * | 2016-04-13 | 2016-06-15 | 深圳市锦瑞生物科技有限公司 | Cell analyzer |
| CN106814183B (en) * | 2016-12-27 | 2019-07-16 | 深圳开立生物医疗科技股份有限公司 | A kind of sheath stream detection system and sheath method of flow control |
| CN108627449B (en) * | 2017-03-23 | 2023-03-31 | 深圳市帝迈生物技术有限公司 | Flow cytometer fluid system and measuring method |
| CN108169104A (en) * | 2017-11-07 | 2018-06-15 | 山东卓越生物技术股份有限公司 | Flow cytometry detection device and method |
| CN109932520A (en) * | 2019-04-19 | 2019-06-25 | 深圳市理邦精密仪器股份有限公司 | Blood test device |
| WO2022099504A1 (en) * | 2020-11-11 | 2022-05-19 | 深圳市科曼医疗设备有限公司 | Liquid adding method for sampling and drawing reagent, and liquid path system therefor |
| CN114778872B (en) * | 2022-06-13 | 2022-11-29 | 深圳市帝迈生物技术有限公司 | Sample analyzer and control method of sample analyzer |
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