CN104067917A - Culture method of aquatic plant in low-concentration phosphorous enriched slightly-polluted water - Google Patents

Culture method of aquatic plant in low-concentration phosphorous enriched slightly-polluted water Download PDF

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CN104067917A
CN104067917A CN201410303089.9A CN201410303089A CN104067917A CN 104067917 A CN104067917 A CN 104067917A CN 201410303089 A CN201410303089 A CN 201410303089A CN 104067917 A CN104067917 A CN 104067917A
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water
illumination
glass jar
deionized water
agar
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CN104067917B (en
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雷春生
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LONGYAN RUIZE ENVIRONMENTAL PROTECTION TECHNOLOGY Co.,Ltd.
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Changzhou University
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Abstract

The invention relates to a culture method of an aquatic plant in low-concentration phosphorous enriched slightly-polluted water, and belongs to the field of sewage treatment. The method comprises the following steps: culturing a batch of water lettuces in a pollution-free pond; removing the outside leaves until 3 to 4 leaves are remained; sterilizing; transferring into a 5L glass tank in which deionized water is contained; irradiating with a 40w fluorescent lamp under room temperature; adding sodium dihydrogen phosphate, agar, glucose, sodium chloride and magnesium sulfate to the deionized water in the glass tank; removing any new leaves of the water lettuces; transferring into another 5L glass tank in which the deionized water is contained; irradiating with the 40w fluorescent lamp under room temperature; transferring into the previous glass tank after irradiating for a certain time; respectively adding certain number of sodium dihydrogen phosphate, agar, glucose, sodium chloride and magnesium sulfate; culturing for 30 days to obtain phosphorous-enriched concentration factors of the water lettuces. According to the culture method, the sewage can be purified, and eutrophication of water mass can be treated.

Description

Low phosphorus water plants breeding method in a kind of enrichment micropollutant water
Technical field
The present invention relates to low phosphorus water plants breeding method in a kind of enrichment micropollutant water, belong to sewage treatment area.
Background technology
In water body, the main source of phosphorus has chemical fertilizer, human and animal excreta, water and soil loss and phosphorus-containing detergent.In city domestic sewage, the phosphorus in phosphorus-containing detergent is the main source of phosphorus in water body.There are some researches show, the phosphorus in lake, reservoir 80% comes from sewage discharge, and the main source of phosphorus is the use of home washings agent, and the pollution intensity of its phosphorus accounts for 50% left and right of total phosphorus pollutional load.Since entering the sixties, along with world population, intensive Big Lakes district is subject to the organic pollution such as nitrogen, phosphorus, has caused many developed countries and regional concern, has started thus worldwide taboo, the motion of limit phosphorus.For a long period, China mainly solves the emission problem of industrial wastewater, and sanitary sewage does not almost carry out any processing and is just directly disposed in water body.The nitrogen and phosphorus pollution problem of drinking water source receives people's concern gradually, the excessive enclosed type body eutrophications such as lake that cause of nitrogen phosphorus, and water quality deterioration can increase the difficulty to water treatment, is difficult to reach sanitary standard for drinking water by conventional treatment.There are some researches show, in water supply network, the N of 0.25mg/L is just enough to make nitrifying bacteria growth and breeding, and nitrifying bacteria can discharge smelly hiding in metabolic process; Excessive nitrate nitrogen can be reduced to nitrous acid nitrogen in people's stomach, forms carcinogenicity material nitrosamine with secondary amine or the effect of phthalein amine in stomach.In effective removal micro polluted source, NITROGEN IN LOW CONCENTRATION phosphorus has become the hot issue of water treatment field.
Make a general survey of dephosphorization technique both domestic and external, chemical precipitation method and coagulation and filtration method, because its operating cost is high, and produce and in a large number easily cause the chemical sludge of secondary pollution and replace biological phosphate-eliminating technology.In traditional removal sewage, the method for nitrogen phosphorus is to utilize nitrogen, phosphorus in algae purification sewage.Utilize the research of nitrogen, phosphorus in algae purification sewage to start early.As spirulina can be in the eutrophic sewage of saliferous well-grown, under illumination condition, just can absorb most of pollutant through gentle agitation.Can provide a kind of comparatively economic method for treating water with algae, weak point is that unicellular alga volume is small, is unfavorable for collecting, and is therefore not suitable for being used as purifying plant in the great water body such as lake, reservoir.
Summary of the invention
The present invention is directed to the chemical sludge that traditional sewage dephosphorization technology easily causes secondary pollution, the problem that dephosphorization algae can not purify in great water body, a kind of method of utilizing water plants to process phosphor in sewage has been proposed, with the method place phosphorus-containing wastewater, not only nitrogen phosphorus is had to good removal ability, and can be rapidly other lot of trace heavy metals in enrichment waste water in large quantities.
For achieving the above object, the concrete technical scheme that the present invention takes is:
(1) in free of contamination pond, cultivate a collection of water lettuce, remove the leaf of outside until remaining 3~4, sterilization, is moved in the glass jar that 5L fills deionized water, and room temperature, with the illumination of 40w fluorescent lamp;
(2) first, in the deionized water of glass jar, add 0.1g~0.4g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(3) in the time that water lettuce newly grows 2 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(4) illumination, after 6 hours, is moved in previous glass jar, adds respectively 0.4g~0.8g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(5) in the time that water lettuce newly grows 3 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(6) illumination, after 12 hours, is moved in previous glass jar, adds respectively 0.8g~1.2g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(7) in the time that water lettuce newly grows 4 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(8) illumination, after 18 hours, is moved in previous glass jar, adds respectively 1.2g~1.6g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(9) in the time that water lettuce newly grows 5 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(10) illumination, after 24 hours, is moved in previous glass jar, adds respectively 1.6g~2.0g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(11) cultivate after 30 days, water lettuce reaches 111000~185500 to the enrichment enrichment factor of phosphorus.
The significant advantage that the present invention has is:
(1) utilize weter hyacinth to process phosphorus-containing wastewater, its processing method is simpler than additive method.
(2) little energy of processing water consumption;
(3) can effectively utilize plant resources.
Embodiment
In free of contamination pond, cultivate a collection of water lettuce, remove the leaf of outside until remaining 3~4, sterilization, is moved in the glass jar that 5L fills deionized water, and room temperature, with the illumination of 40w fluorescent lamp; First, in the deionized water of glass jar, add 0.1g~0.4g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate; In the time that water lettuce newly grows 2 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 6 hours, be moved in previous glass jar, add respectively 0.4g~0.8g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate; In the time that water lettuce newly grows 3 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 12 hours, be moved in previous glass jar, add respectively 0.8g~1.2g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate; In the time that water lettuce newly grows 4 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 18 hours, be moved in previous glass jar, add respectively 1.2g~1.6g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate; In the time that water lettuce newly grows 5 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 24 hours, be moved in previous glass jar, add respectively 1.6g~2.0g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate; Cultivate after 30 days, water lettuce reaches 111000~185500 to the enrichment enrichment factor of phosphorus.
Example 1
In free of contamination pond, cultivate a collection of water lettuce, remove the leaf of outside until remaining 3~4, sterilization, is moved in the glass jar that 5L fills deionized water, and room temperature, with the illumination of 40w fluorescent lamp; First, in the deionized water of glass jar, add 0.1g sodium dihydrogen phosphate, 2g agar, 2g glucose sugar, 2g sodium chloride and 2g magnesium sulfate; In the time that water lettuce newly grows 2 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 6 hours, be moved in previous glass jar, add respectively 0.4g sodium dihydrogen phosphate, 2g agar, 2g glucose sugar, 2g sodium chloride and 2g magnesium sulfate; In the time that water lettuce newly grows 3 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 12 hours, be moved in previous glass jar, add respectively 0.8g sodium dihydrogen phosphate, 2g agar, 2g glucose sugar, 2g sodium chloride and 2g magnesium sulfate; In the time that water lettuce newly grows 4 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 18 hours, be moved in previous glass jar, add respectively 1.2g sodium dihydrogen phosphate, 2g agar, 2g glucose sugar, 2g sodium chloride and 2g magnesium sulfate; In the time that water lettuce newly grows 5 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 24 hours, be moved in previous glass jar, add respectively 1.6g sodium dihydrogen phosphate, 2g agar, 2g glucose sugar, 2g sodium chloride and 2g magnesium sulfate; Cultivate after 30 days, water lettuce reaches 111000 to the enrichment enrichment factor of phosphorus.
Example 2
In free of contamination pond, cultivate a collection of water lettuce, remove the leaf of outside until remaining 3~4, sterilization, is moved in the glass jar that 5L fills deionized water, and room temperature, with the illumination of 40w fluorescent lamp; First, in the deionized water of glass jar, add 0.25g sodium dihydrogen phosphate, 2.5g agar, 2.5g glucose sugar, 2.5g sodium chloride and 2.5g magnesium sulfate; In the time that water lettuce newly grows 2 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 6 hours, be moved in previous glass jar, add respectively 0.6g sodium dihydrogen phosphate, 2.5g agar, 2.5g glucose sugar, 2.5g sodium chloride and 2.5g magnesium sulfate; In the time that water lettuce newly grows 3 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 12 hours, be moved in previous glass jar, add respectively 1.0g sodium dihydrogen phosphate, 2.5g agar, 2.5g glucose sugar, 2.5g sodium chloride and 2.5g magnesium sulfate; In the time that water lettuce newly grows 4 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 18 hours, be moved in previous glass jar, add respectively 1.4g sodium dihydrogen phosphate, 2.5g agar, 2.5g glucose sugar, 2.5g sodium chloride and 2.5g magnesium sulfate; In the time that water lettuce newly grows 5 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 24 hours, be moved in previous glass jar, add respectively 1.8g sodium dihydrogen phosphate, 2.5g agar, 2.5g glucose sugar, 2.5g sodium chloride and 2.5g magnesium sulfate; Cultivate after 30 days, water lettuce reaches 148250 to the enrichment enrichment factor of phosphorus.
Example 3
In free of contamination pond, cultivate a collection of water lettuce, remove the leaf of outside until remaining 3~4, sterilization, is moved in the glass jar that 5L fills deionized water, and room temperature, with the illumination of 40w fluorescent lamp; First, in the deionized water of glass jar, add 0.4g sodium dihydrogen phosphate, 3g agar, 3g glucose sugar, 3g sodium chloride and 3g magnesium sulfate; In the time that water lettuce newly grows 2 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 6 hours, be moved in previous glass jar, add respectively 0.8g sodium dihydrogen phosphate, 3g agar, 3g glucose sugar, 3g sodium chloride and, 3g magnesium sulfate; In the time that water lettuce newly grows 3 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 12 hours, be moved in previous glass jar, add respectively 1.2g sodium dihydrogen phosphate, 3g agar, 3g glucose sugar, 3g sodium chloride and 3g magnesium sulfate; In the time that water lettuce newly grows 4 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 18 hours, be moved in previous glass jar, add respectively 1.6g sodium dihydrogen phosphate 3g agar, 3g glucose sugar, 3g sodium chloride and 3g magnesium sulfate; In the time that water lettuce newly grows 5 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp; After illumination 24 hours, be moved in previous glass jar, add respectively 2.0g sodium dihydrogen phosphate, 3g agar, 3g glucose sugar, 3g sodium chloride and 3g magnesium sulfate; Cultivate after 30 days, water lettuce reaches 185500 to the enrichment enrichment factor of phosphorus.

Claims (1)

1. a low phosphorus water plants breeding method in enrichment micropollutant water, is characterized in that:
(1) in free of contamination pond, cultivate a collection of water lettuce, remove the leaf of outside until remaining 3~4, sterilization, is moved in the glass jar that 5L fills deionized water, and room temperature, with the illumination of 40w fluorescent lamp;
(2) first, in the deionized water of glass jar, add 0.1g~0.4g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(3) in the time that water lettuce newly grows 2 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(4) illumination, after 6 hours, is moved in previous glass jar, adds respectively 0.4g~0.8g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(5) in the time that water lettuce newly grows 3 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(6) illumination, after 12 hours, is moved in previous glass jar, adds respectively 0.8g~1.2g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(7) in the time that water lettuce newly grows 4 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(8) illumination, after 18 hours, is moved in previous glass jar, adds respectively 1.2g~1.6g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(9) in the time that water lettuce newly grows 5 leaves, pull out its new linear leaf, move in the glass jar that another 5L fills deionized water, room temperature, with the illumination of 40w fluorescent lamp;
(10) illumination, after 24 hours, is moved in previous glass jar, adds respectively 1.6g~2.0g sodium dihydrogen phosphate, 2~3g agar, 2~3g glucose sugar, 2~3g sodium chloride and 2~3g magnesium sulfate;
(11) cultivate after 30 days, water lettuce reaches 111000~185500 to the enrichment enrichment factor of phosphorus.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104925953A (en) * 2015-06-11 2015-09-23 常州大学 Method for culturing aquatic plant for removing trace organomercury in wastewater

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58180293A (en) * 1982-04-19 1983-10-21 Kumagai Gumi Ltd Treatment of sewage with activated sludge
KR20050045116A (en) * 2003-11-10 2005-05-17 김진종 Development of equipment for advanced sewage wastewater treatment by eichhornia crassipes
US20050178723A1 (en) * 1997-04-01 2005-08-18 Science Applications International Corporation Integrated system and method for purifying water, producing pulp and paper, and improving soil quality
CN101023736A (en) * 2007-03-20 2007-08-29 中国科学院武汉病毒研究所 External regenerating and breeding water lettuce
CN101259973A (en) * 2007-03-09 2008-09-10 上海蒲谷绿化工程有限公司 Method for purifying water by using floating-field plants
CN101473742A (en) * 2009-02-18 2009-07-08 沃邦(江苏)生态肥业有限公司 Method for plating water hyacinth with control in open water
CN103435165A (en) * 2013-09-18 2013-12-11 浙江省农业科学院 Method for blocking and absorbing nitrogen and phosphorus flowing away from paddy field by utilizing water discharging channel

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58180293A (en) * 1982-04-19 1983-10-21 Kumagai Gumi Ltd Treatment of sewage with activated sludge
US20050178723A1 (en) * 1997-04-01 2005-08-18 Science Applications International Corporation Integrated system and method for purifying water, producing pulp and paper, and improving soil quality
KR20050045116A (en) * 2003-11-10 2005-05-17 김진종 Development of equipment for advanced sewage wastewater treatment by eichhornia crassipes
CN101259973A (en) * 2007-03-09 2008-09-10 上海蒲谷绿化工程有限公司 Method for purifying water by using floating-field plants
CN101023736A (en) * 2007-03-20 2007-08-29 中国科学院武汉病毒研究所 External regenerating and breeding water lettuce
CN101473742A (en) * 2009-02-18 2009-07-08 沃邦(江苏)生态肥业有限公司 Method for plating water hyacinth with control in open water
CN103435165A (en) * 2013-09-18 2013-12-11 浙江省农业科学院 Method for blocking and absorbing nitrogen and phosphorus flowing away from paddy field by utilizing water discharging channel

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104925953A (en) * 2015-06-11 2015-09-23 常州大学 Method for culturing aquatic plant for removing trace organomercury in wastewater

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