CN103893069A - Preparation method and application of effective ingredients of bletilla formosana - Google Patents
Preparation method and application of effective ingredients of bletilla formosana Download PDFInfo
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- CN103893069A CN103893069A CN201410120863.2A CN201410120863A CN103893069A CN 103893069 A CN103893069 A CN 103893069A CN 201410120863 A CN201410120863 A CN 201410120863A CN 103893069 A CN103893069 A CN 103893069A
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Abstract
The invention relates to a preparation method and application of effective ingredients of bletilla formosana, and belongs to the field of extraction of effective ingredients of traditional Chinese medicament. The preparation method comprises the following steps: a, crushing bletilla formosana, and extracting with water to obtain an extracting solution; b, concentrating the extracting solution to 3-5 times of the weight of the bletilla formosana to obtain a concentrate; and c, adding the concentrate into 80-90% ethanol to precipitate, filtering to obtain a filter cake, washing the filter cake to remove ethanol, diluting with water, and drying to obtain the effective ingredients of bletilla formosana. The preparation method has the beneficial effects that the effective ingredients of bletilla formosana, obtained by utilizing the preparation method, are rich in polysaccharide and other effective ingredients, have the activities of moistening and hydrating, protecting skin, delaying skin senescence, resisting oxidation, sterilizing and disinfecting and the like, and can be used as additives or raw materials of foods, health foods, cosmetics and medicines.
Description
Technical field
The invention belongs to Chinese medicine active component and extract field, be specifically related to a kind of preparation method and purposes of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
Background technology
Little Pseudobulbus Bletillae (Rhizoma Bletillae) ［ Bletilla formosana ］ is the orchid family Pseudobulbus Bletillae (Rhizoma Bletillae) platymiscium, is distributed in Shaanxi, Sichuan, Yunnan, Guizhou, Guangxi, Taiwan, and the profile of little Pseudobulbus Bletillae (Rhizoma Bletillae) and Pseudobulbus Bletillae (Rhizoma Bletillae) is approximate, and medical material obviously slight of stature is withered, and there is vertical wrinkle on surface, without thick moisture feeling.Have no at present the research report of its chemical composition, and the research of congener Pseudobulbus Bletillae (Rhizoma Bletillae) is more, it is rich in bletilla polysaccharide, bletilla polysaccharide has moisturizing, antiinflammatory, coagulant blood, antiviral, antitumor, the biologic activity such as antioxidation, as natural macromolecular material, there are function slow-releasing, local anelasticity, self degradability, nonirritant, the advantage such as have no side effect.Bletilla polysaccharide is holding out broad prospects aspect development of new preparation at present, as food thickening agent, stabilizing agent, degradable package material, wound adjuvant, biodegradable stent material etc.; Also be widely used in daily chemical products simultaneously.But up to now, in prior art there are no the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component and active report.
Summary of the invention
The object of this invention is to provide a kind of preparation method and its usage of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
Little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of the present invention, its preparation process comprises the following steps:
A, get little Pseudobulbus Bletillae (Rhizoma Bletillae), fragmentation, water extracts, and obtains extracting solution; Preferably, for improving the stripping of effective ingredient, increase the conversion ratio extracting, broken order number is 80 orders.
B, described extracting solution is concentrated into 3～5 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, obtains concentrated solution;
C, described concentrated solution is added to mass percent concentration is 80%～90% ethanol precipitation, filters, and obtains filter cake, washes away the ethanol on described filter cake, and thin up is dry, obtains little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
Preferably, the number of times extracting in described step a is 1～4 time, and each time of extracting is 0.5～4 hour, and the weight that adds water while at every turn extraction is 5～20 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.Particularly preferred, the number of times of extraction is 2 times, 3 times; The time of extracting is 1 hour, 2 hours, 3 hours; The weight that adds water when each extraction is 8 times, 10 times, 12 times, 14 times, 16 times, 18 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.
Preferably, in described step b, extracting solution is concentrated into 4.3 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.
Preferably, in described step c, the mass percent concentration of ethanol is 85%.
Preferably, the volume that adds of described ethanol is 4～6 times of concentrated solution volume.Particularly preferred, the volume that adds of described ethanol is 5 times of concentrated solution volume.
Preferably, in described step c, the addition of water is 3～5 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.Particularly preferred, in described step c, the addition of water is 4 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.
Preferably, the dry one that adopts lyophilization or spray in being dried in described step c.
The little Pseudobulbus Bletillae (Rhizoma Bletillae) active component that the present invention also provides a kind of preparation method of described little Pseudobulbus Bletillae (Rhizoma Bletillae) active component to obtain.
The present invention also provides a kind of described little Pseudobulbus Bletillae (Rhizoma Bletillae) active component in the application of preparing in moisture-keeping cosmetics.
In the time that the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of gained of the present invention is applied to human or animal as the raw material of cosmetics or adjuvant, they can give with itself, and the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component above-mentioned by the present invention is directly applied to user with original shape; Or can be to contain for example 0.1%～99.9% little Pseudobulbus Bletillae (Rhizoma Bletillae) active component and other supplementary material that can form cosmetics to be used in conjunction with.
Beneficial effect of the present invention is: utilize in the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of preparation method gained of the present invention and be rich in the effective ingredient such as polysaccharide; there is moisturizing moisturizing, protection skin, delay skin aging, antioxidation and anti-inflammation isoreactivity, can use for the additive of food, health food, cosmetics, medicine or raw material.
Brief description of the drawings
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, below the accompanying drawing of required use during embodiment is described is briefly described.
Fig. 1 is the test pattern that affects that in the embodiment of the present invention 1, the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of gained suppresses hyaluronidase.
Detailed description of the invention
For making those skilled in the art understand in detail production technology of the present invention and technique effect, further introduce application of the present invention and technique effect with concrete production instance below.
Embodiment 1:
A, get little Pseudobulbus Bletillae (Rhizoma Bletillae), be crushed to 60 orders, add the water extraction 3 times of 8 times of weight, extract merge extractive liquid, at every turn 3 hours;
B, extracting solution is concentrated into 4.3 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, obtains concentrated solution;
C, concentrated solution is added to mass percent concentration is 85% ethanol precipitation, filters, and obtains filter cake, washes away the ethanol on filter cake, adds the water dilution of 4 times of amounts of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, and lyophilization, obtains little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
Embodiment 2:
A, get little Pseudobulbus Bletillae (Rhizoma Bletillae), be crushed to 80 orders, add the water extraction 1 time of 20 times of weight, extract 4 hours, obtain extracting solution;
B, extracting solution is concentrated into 5 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, obtains concentrated solution;
C, concentrated solution is added to mass percent concentration is 80% ethanol precipitation, filters, and obtains filter cake, washes away the ethanol on filter cake, adds the water dilution of 3 times of amounts of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, and spraying is dry, obtains little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
Embodiment 3:
A, get little Pseudobulbus Bletillae (Rhizoma Bletillae), be crushed to 100 orders, add the water extraction 4 times of 5 times of weight, extract merge extractive liquid, at every turn 0.5 hour;
B, extracting solution is concentrated into 3 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, obtains concentrated solution;
C, concentrated solution is added to mass percent concentration is 90% ethanol precipitation, filters, and obtains filter cake, washes away the ethanol on filter cake, adds the water dilution of 5 times of amounts of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, and lyophilization, obtains little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
Embodiment 4:
Get the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of embodiment 1 gained, add appropriate bathypelagic fish oil and spice and make emulsion.Give at random 3 months on probation of 100 clients by emulsion, 90% people thinks, compares the skin not using time of the present invention, and the wrinkle at face, canthus obviously reduces, and it is tender that skin becomes, and the colour of skin obviously improves.
Embodiment 5:
Get the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of embodiment 2 gained, add appropriate distilled water, make water liquid.This water liquid can be used for the diseases such as chafing.
For proving beneficial effect of the present invention, spy carries out following test using the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component of embodiment 1 gained as sample:
Moisturizing active testing (suppressing hyaluronic acid activity)
Experimental principle: hyaluronic acid is a kind of acid mucopolysaccharide, has special water retention, is the best material of occurring in nature moisture retention of finding at present, is called as desirable nature moisturizing factor (Naturalmoisturizing factor, NMF).And hyaluronidase is to make hyaluronic acid produce the general name of degraded effect enzyme, hyaluronidase too much in body can reduce hyaluronic activity.Therefore by can judge the effect of moisturizing moisturizing to the inhibitory action of hyaluronidase.
Experimental technique
Get 0.1mL0.25mmol/L CaCl2 solution and 0.5mL hyaluronidase liquid (300u/mL) in 37 DEG C of heat insulating culture 20min; To add respectively mass concentration be 2% little Pseudobulbus Bletillae (Rhizoma Bletillae) active component solution 50ul, 100ul, 200ul and 0.5ml to the mass concentration containing little Pseudobulbus Bletillae (Rhizoma Bletillae) active component be 1.5%, 3%, 6%, 15%, continue 37 DEG C of heat insulating culture 20min; Add 0.5mL sodium hyaluronate solution (0.5mg/mL), in 37 DEG C of insulation 30min, room temperature is placed 5min again; Then add 0.1mL0.4mol/L NaOH solution and 0.5mL acetylacetone,2,4-pentanedione solution (0.7mL acetylacetone,2,4-pentanedione is dissolved in 1% Na2CO3 solution and makes), be placed in after boiling water bath heats 15min and carry out cooling 5min with frozen water immediately; Add Ehrlich's reagent 1.0mL (the p-dimethylaminobenzaldehyde of 0.8mL is dissolved in 15mL concentrated hydrochloric acid and 15mL dehydrated alcohol) also to dilute with 3.0mL dehydrated alcohol, room temperature is placed 20min colour developing, with its absorbance of spectrophotometric determination.
Sample is as follows to the measure and calculation formula of hyaluronic acid enzyme inhibition rate:
(A-B)-(C-D)/(A-B)*100%
Wherein: A is that pH is the absorbance that 5.6 acetate buffer solution replaces enzyme liquid and little Pseudobulbus Bletillae (Rhizoma Bletillae) sample solution
B is that pH is the absorbance that 5.6 acetate buffer solution replaces little Pseudobulbus Bletillae (Rhizoma Bletillae) sample solution
C is the absorbance of variable concentrations sample solution
D is the absorbance that replaces enzyme liquid with acetate buffer solution
Test result is shown in Fig. 1, known: little Pseudobulbus Bletillae (Rhizoma Bletillae) active component exists good inhibition to hyaluronic acid, and suppression ratio can reach 50% left and right, and suppression ratio presents certain concentration dependency, substantially reaches concentration saturated in the time that addition is 6% left and right.The test of above-mentioned moisturizing activity can be referring to document: Zhao Hua, etc. Radix et Caulis Opuntiae Dillenii lyophilized powder extraction process is optimized and antiallergic efficacy detection. food industry science and technology, 2010,5:305-307.
Comparative example 1:
Get little Pseudobulbus Bletillae (Rhizoma Bletillae) 95% ethanol extraction, residue is used water extraction 3 times again, extracts 2 hours at every turn, merges aqueous extract, concentrated, dry, obtains little Pseudobulbus Bletillae (Rhizoma Bletillae) extract.
Comparative example 2-5:
Except extracting solution in step b is concentrated into 1 times, 2 times, 6 times and 7 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, all the other modes in the same manner as in Example 1 are implemented, and obtain comparative example 2,3,4,5.
Comparative example 6-8:
Except becoming 60%, 70%, 90% for the concentration of alcohol precipitating in step c, all the other modes in the same manner as in Example 1 are implemented, and obtain comparative example 6,7 and 8.
For verifying technique effect of the present invention, embodiment 1-3 and comparative example 1-8 are carried out to the detection of polyoses content and the test of moisturizing activity, the results are shown in following table:
| Product | Polyoses content | To hyaluronic suppression ratio |
| Embodiment 1 | 45mg/ml | 52% |
| Embodiment 2 | 50mg/ml | 51% |
| |
48mg/ml | 53% |
| Comparative example 1 | 18mg/ml | 8% |
| Comparative example 2 | 8mg/ml | 13% |
| Comparative example 3 | 11mg/ml | 19% |
| Comparative example 4 | 21mg/ml | 23% |
| Comparative example 5 | 23mg/ml | 25% |
| Comparative example 6 | 33mg/ml | 28% |
| Comparative example 7 | 29mg/ml | 24% |
| Comparative example 8 | 34mg/ml | 27% |
The detection of above-mentioned polyoses content, under employing " Chinese Pharmacopoeia " version Ganoderma item in 2010, the mensuration of polysaccharide is carried out.
Finally it should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to above-described embodiment, those skilled in the art are to be understood that, still can modify or be equal to replacement the present invention, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in claim scope of the present invention.
Claims (9)
1. a preparation method for little Pseudobulbus Bletillae (Rhizoma Bletillae) active component, is characterized in that, comprises the following steps:
A, get little Pseudobulbus Bletillae (Rhizoma Bletillae), fragmentation, water extracts, and obtains extracting solution;
B, described extracting solution is concentrated into 3～5 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight, obtains concentrated solution;
C, described concentrated solution is added to mass percent concentration is 80%～90% ethanol precipitation, filters, and obtains filter cake, washes away the ethanol on described filter cake, and thin up is dry, obtains little Pseudobulbus Bletillae (Rhizoma Bletillae) active component.
2. the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component according to claim 1, it is characterized in that: the number of times extracting in described step a is 1～4 time, each time of extracting is 0.5～4 hour, and the weight that adds water while at every turn extraction is 5～20 times of little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.
3. the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component according to claim 1, is characterized in that: in described step b, extracting solution is concentrated into 4.3 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.
4. the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component according to claim 1, is characterized in that: in described step c, the mass percent concentration of ethanol is 85%.
5. the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component according to claim 4, is characterized in that: the volume that adds of described ethanol is 4～6 times of concentrated solution volume.
6. the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component according to claim 1, is characterized in that: in described step c, the addition of water is 3～5 times of described little Pseudobulbus Bletillae (Rhizoma Bletillae) weight.
7. the preparation method of little Pseudobulbus Bletillae (Rhizoma Bletillae) active component according to claim 1, is characterized in that: the dry one that adopts lyophilization or spray in being dried in described step c.
8. the little Pseudobulbus Bletillae (Rhizoma Bletillae) active component that the preparation method based on little Pseudobulbus Bletillae (Rhizoma Bletillae) active component described in any one in claim 1-7 obtains.
9. according to claim 8 little Pseudobulbus Bletillae (Rhizoma Bletillae) active component is in the application of preparing in moisture-keeping cosmetics.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969438A (en) * | 2018-08-23 | 2018-12-11 | 广东幸美化妆品股份有限公司 | Contain bletilla striata extract, white flower papaya extractives and anti-wrinkle cream of Herba Agrimoniae extract and preparation method thereof |
| CN109498529A (en) * | 2017-09-15 | 2019-03-22 | 伽蓝（集团）股份有限公司 | The application of bletilla striata extract |
| CN113350248A (en) * | 2021-05-27 | 2021-09-07 | 贵州省农作物品种资源研究所 | Preparation and application method of bletilla striata gel freeze-dried powder skin moisturizer |
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| CN101496784A (en) * | 2009-03-13 | 2009-08-05 | 云南省药物研究所 | Chinese medicine shower lotion |
| CN101849899A (en) * | 2009-12-24 | 2010-10-06 | 上海原尚草化妆品有限公司 | Chinese medicinal compound extract and cosmetics comprising same as active ingredient |
| CN102258442A (en) * | 2011-07-15 | 2011-11-30 | 广州市娇兰化妆品有限公司 | Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product |
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2014
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Patent Citations (4)
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| CN101496784A (en) * | 2009-03-13 | 2009-08-05 | 云南省药物研究所 | Chinese medicine shower lotion |
| CN101849899A (en) * | 2009-12-24 | 2010-10-06 | 上海原尚草化妆品有限公司 | Chinese medicinal compound extract and cosmetics comprising same as active ingredient |
| CN102258442A (en) * | 2011-07-15 | 2011-11-30 | 广州市娇兰化妆品有限公司 | Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109498529A (en) * | 2017-09-15 | 2019-03-22 | 伽蓝（集团）股份有限公司 | The application of bletilla striata extract |
| CN108969438A (en) * | 2018-08-23 | 2018-12-11 | 广东幸美化妆品股份有限公司 | Contain bletilla striata extract, white flower papaya extractives and anti-wrinkle cream of Herba Agrimoniae extract and preparation method thereof |
| CN108969438B (en) * | 2018-08-23 | 2021-09-21 | 广东幸美化妆品股份有限公司 | Anti-wrinkle cream containing bletilla striata extract, chaenomeles speciosa extract and hairyvein agrimony extract and preparation method thereof |
| CN113350248A (en) * | 2021-05-27 | 2021-09-07 | 贵州省农作物品种资源研究所 | Preparation and application method of bletilla striata gel freeze-dried powder skin moisturizer |
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Application publication date: 20140702 |