CN103749152A - Shitake mushroom planting method - Google Patents
Shitake mushroom planting method Download PDFInfo
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- CN103749152A CN103749152A CN201310749291.XA CN201310749291A CN103749152A CN 103749152 A CN103749152 A CN 103749152A CN 201310749291 A CN201310749291 A CN 201310749291A CN 103749152 A CN103749152 A CN 103749152A
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Abstract
The invention discloses a shitake mushroom planting method. A main formula comprises cotton seed hulls 40kg, wood chips 40kg, wheat bran 15kg, corn flour 5kg, calcium superphosphate 0.5kg, sugar 0.5kg and water 55%-59% (w/w), wherein the proportion of the materials to the water is 1:0.9-1.1. Commonly-used culture medium raw materials include wood chips, cotton seed hulls and the like. The culture medium raw materials can be contained with bottles and can be also contained with polypropylene plastic bags. A bagging mode has the advantages of enabling the material charging amount to be large, facilitating carrying and enabling seed taking to be easy and the like. Shitake mushrooms planted by adopting the shitake mushroom planting method are large in cap diameter, are in flat-hemispheric shapes, are dark brown or dark cinnamon and has dark-color flakes, thick pulp and high nutritive value.
Description
Technical field
The invention belongs to growing vegetables technical field, particularly relate to a kind of implantation methods of mushroom.
Background technology
Mushroom fruiting body Dan Sheng, grow thickly or all living creatures.Cap is circular, and 5-10cm, reaches 20cm sometimes conventionally, and surperficial dark brown, crineous, had dark scale.During children, edge is involute, and the fine hair of adularescent or yellow-white, disappears along with growth.Below cap, have velum, after break, form incomplete collarium.Aging bonnet edge warp, cracking.The curved life of lamella, white, raw or wilfully in stem, 3-6cm * 1-1.5cm, interior reality, the above part white of collarium, the following part brown of collarium.Spore print white, basidiospore is colourless under the microscope, ellipse, 5-7um * 3.4-4um, nucleated mycelium has clamp connection.Mushroom claims again fragrant bacterium, dried mushroom.Due to its delicious flavour, the fragrance people that oozes, nutritious, not only rank on straw mushroom, flat mushroom, and have the reputation of " plant queen ", be one of " mountain delicacy ".Mushroom has high protein, low fat, polysaccharide, several amino acids and multivitamin nutritional characteristic.Owing to containing agaritine rare in normal food, tricholomic acid etc. in mushroom, therefore taste is delicious especially.Mushroom is to have high protein, low fat, polysaccharide, several amino acids and multivitamin mushroom food.1. improve body's immunity: lentinan can improve the phagocytic function of Turnover of Mouse Peritoneal Macrophages, also can promote the lymphocytic generation of T, and improves the lymphocytic killing activity of T.2. delay senility: the water extract of mushroom has scavenging action to hydrogen peroxide, the hydrogen peroxide in body is had to certain elimination effect.3. cancer-resisting: mushroom cap partly contains the ribonucleic acid of duplex structure, enters after human body, can produce the interferon with antitumaous effect.4. hypotensive, reducing blood lipid, norcholesterol: in mushroom, contain purine, choline, tyrosine, oxidase and some nucleic acid substances, can play the effect of hypotensive, norcholesterol, reducing blood lipid, again can prevention of arterial sclerosis, the disease such as cirrhosis.5. mushroom also plays therapeutic action to diabetes, pulmonary tuberculosis, catarrhal jaundice, neuritis etc., can be used for again indigestion, constipation etc.Mushroom contains vitamin C, can play and reduce cholesterol, hypotensive effect.Mushroom juice can replace depressor completely, and is free from side effects.The black alcohol of wheat that has a kind of general vegetables to lack in mushroom, it can be converted into vitamin D, promotes the absorption of calcium in body, and can strengthen the ability of human body resist the disease.
Summary of the invention
The invention discloses a kind of implantation methods of mushroom, it is characterized in that being undertaken by following step:
(1) formula: cotton seed hull 40kg, wood chip 40kg, wheat bran 15kg, corn flour 5kg, superphosphate 0.5kg, sugared 0.5kg, water 55%～59%(w/w), material is 1:0.9～1.1 with water ratio.
(2) spice: cotton seed hull, wood chip and wheat bran and corn flour are first mixed; Separately superphosphate, sugar are stirred, then with agitator, the two stirred in water;
(3) pack: by the composts or fertilisers of cultivating of mixing, pack polypropylene plastics pocket into, bacterium bag specification: 17 * 35 * 0.045cm, installs material at 6h～8h;
(4) sealing: first a resistant to elevated temperatures plastics neck ring is mounted on plastic bag mouth, turnup sack, and by tampon on neck stopper, then tampon is wrapped with the high pressure resistant plastic film of a fritter, with cotton rope, tie up solid, sterilizing immediately;
(5) sterilizing: under 0.14～0.15MPa, 2h～3h, normal pressure 16h～24h carries out moist heat sterilization;
(6) inoculation: will
mushroom strainbag is put into transfer room, or in inoculating hood, ultraviolet lamp disinfection sterilization, inoculation cultivation
mushroom strain, inoculum concentration is 15 bags of/bottle of original seeds;
(7) send out bacterium: inoculate completely, cultivated species is put into ' # ' character code buttress under the condition of 25 ℃ of left and right and sends out a bacterium; After 30d～45d cultivates, mycelium covers with cultivation bag.
The implantation methods of mushroom of the present invention, wherein the described inoculation of step (6) also can be made a call to 3 caves in every bag of front, and stagger and make a call to 2 caves in the back side, cave is dark≤1.0cm, and footpath, cave 1.5～2.0cm, rapidly inoculation, and sealing cave with 3.5 * 3.5cm adhesive tape, every bottle of original seed can connect 80～100 caves, approximately 20 bacterium rods.
Cultivated species is by original seed thalline, to transplant propagation to form again.The composition of its preparation method and medium and its original seed are basic identical.Conventional culture medium raw material mostly is wood chip, cotton seed hull etc.Its composts or fertilisers of cultivating can be with bottled, also available polypropylene plastics pocket.Packed to have charging many, are convenient to carrying and easily get the features such as kind.But, while using plastic sack, to carefully check, in case there are sand holes to pollute.
The more detailed implantation methods of the present invention is as follows:
Formula: cotton seed hull 40kg, wood chip 40kg, wheat bran 15kg, corn flour 5kg, superphosphate 0.5kg, sugared 0.5kg.Water 55%～59%, material is 1:0.9～1.1 with water ratio.
Spice: according to production of hybrid seeds needs, weighed various raw materials by nutrient component proportioning, then cotton seed hull, wood chip and wheat bran and corn flour have first been mixed; Separately superphosphate, sugar etc. is stirred in water, then with agitator, the two is stirred.
Medium (culture medium) water content: after composts or fertilisers of cultivating is mixed, with hand, grab a composts or fertilisers of cultivating and be held in the hand, firmly clench, from finger seam, have watermark, anhydrous oozing now, the material that spreads one's fingers is not agglomerating, and this moisture content in medium approximately 55%～58% is the most suitable.
Pack: by the composts or fertilisers of cultivating of mixing, pack polypropylene plastics pocket into.Bacterium bag specification: (17 * 35 * 0.045) cm, generally can fill siccative 500g left and right.Adopt machinery pack.Require consolidation and flexible, and tightly expect a mouthful place with hand.Being preferably in 6h～8h installs material.
Sealing: first a resistant to elevated temperatures plastics neck ring is mounted on plastic bag mouth, turnup sack, and by tampon on neck stopper, then tampon is wrapped with the high pressure resistant plastic film of a fritter, with cotton rope, tie up solid, sterilizing immediately.
Sterilizing (steriliztion): the model of selecting autoclave according to grower's plantation amount.Large-size horizontal high-pressure steam sterilizing pan, once can sterilizing reach thousands of bags.Sterilizing methods, due to the using method of various model autoclaves difference slightly, should strictly operate by the requirement of autoclave operation instructions.General Requirements under 0.14～0.15MPa, 2h～3h, normal pressure 16h～24h carries out moist heat sterilization.Autoclaving no matter, or normal-pressure sterilization drains cold air most critical in time, guarantees that sterilization time and temperature are most important.
Inoculation (inoculation): bacterial classification (mushroom strain) bag is put into transfer room, or in inoculating hood, ultraviolet lamp disinfection sterilization.Inoculation cultivated species, a people uses inoculation spoon under aseptic technique, or tweezers are got appropriate original seed thalline greatly, rapidly original seed are received in bag, fill in rapidly tampon, be sack.Inoculum concentration is 15 bags of/bottle of original seeds.Also can make a call to 3 caves in every bag of front, stagger and make a call to 2 caves in the back side, and cave is dark≤1.0cm, footpath, cave 1.5～2.0cm, and inoculation rapidly, and stick with glue band (3.5 * 3.5cm) and seal cave.Every bottle of original seed can connect 80～100 caves, approximately 20 bacterium rods.
Send out bacterium inoculation complete, cultivated species is put into ' # ' character code buttress under the condition of 25 ℃ of left and right and sends out a bacterium.In strict accordance with four conditions sending out bacterium, secretly cultivate; Environment clean dried; Suitable temperature; Normal ventilation.After 30d～45d cultivates, mycelium can cover with cultivation bag.Sending out a bacterium stage, if having Huang, variegated spot or the bacterium colony such as green, black at bacterial classification place, bacterial classification pollution be described; If there is pollution at place not long mycelia, illustrates on bacterium bag and have trachoma or sterilizing not thorough.
Adopt the mushroom of method plantation of the present invention and the comparison of conventional method:
| Relatively | The inventive method plantation | Conventional method plantation |
| Mushroom fruiting body | Dan Sheng | Dan Sheng |
| CapDiameter | 10-12cm | 5-8 cm |
| Surface color | Dark brown is to dark cinnamon | Shallow Brown |
| Bacterial context | White | Yellow-white |
| Spore | It is smooth, colourless, | Faint yellow |
| Mycelia | White, fine hair shape is dense | Sparse |
Conclusion:
The mushroom that adopts method plantation of the present invention, bacteria cover diameter is large, and proterties mostly is flat hemispherical, and dark brown, to dark cinnamon, has dark scale, and bacterial context is thick, has very high nutritive value.
Embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.Cotton seed hull of the present invention, wood chip, wheat bran, corn flour, superphosphate, sugar, etc. various raw materials all have commercially available.Wherein
mushroom strainbuy in
yutai County, Shandong Province edible mushroom research institute.
Embodiment 1
(1) formula: cotton seed hull 40kg, wood chip 40kg, wheat bran 15kg, corn flour 5kg, superphosphate 0.5kg, sugared 0.5kg, water 55%(w/w), material is 1:0.9 with water ratio.
(2) spice: cotton seed hull, wood chip and wheat bran and corn flour are first mixed; Separately superphosphate, sugar are stirred, then with agitator, the two stirred in water;
(3) pack: by the composts or fertilisers of cultivating of mixing, pack polypropylene plastics pocket into, bacterium bag specification: 17 * 35 * 0.045cm, installs material at 6h;
(4) sealing: first a resistant to elevated temperatures plastics neck ring is mounted on plastic bag mouth, turnup sack, and by tampon on neck stopper, then tampon is wrapped with the high pressure resistant plastic film of a fritter, with cotton rope, tie up solid, sterilizing immediately;
(5) sterilizing: under 0.14MPa, 2h, normal pressure 16h carries out moist heat sterilization;
(6) inoculation: will
mushroom strainbag is put into transfer room, or in inoculating hood, ultraviolet lamp disinfection sterilization, inoculation cultivation
mushroom strain, inoculum concentration is 15 bags of/bottle of original seeds;
(7) send out bacterium: inoculate completely, cultivated species is put into ' # ' character code buttress under the condition of 25 ℃ of left and right and sends out a bacterium; After 30d cultivates, mycelium covers with cultivation bag.Wherein the described inoculation of step (6) also can be made a call to 3 caves in every bag of front, staggers and make a call to 2 caves in the back side, and cave is dark≤1.0cm, footpath, cave 1.5cm, inoculation rapidly, and seal cave with 3.5 * 3.5cm adhesive tape, every bottle of original seed can connect 80 caves, approximately 20 bacterium rods.
Embodiment 2
(1) formula: cotton seed hull 40kg, wood chip 40kg, wheat bran 15kg, corn flour 5kg, superphosphate 0.5kg, sugared 0.5kg, water 59%(w/w), material is 1:1.1 with water ratio.
(2) spice: cotton seed hull, wood chip and wheat bran and corn flour are first mixed; Separately superphosphate, sugar are stirred, then with agitator, the two stirred in water;
(3) pack: by the composts or fertilisers of cultivating of mixing, pack polypropylene plastics pocket into, bacterium bag specification: 17 * 35 * 0.045cm, installs material at 8h;
(4) sealing: first a resistant to elevated temperatures plastics neck ring is mounted on plastic bag mouth, turnup sack, and by tampon on neck stopper, then tampon is wrapped with the high pressure resistant plastic film of a fritter, with cotton rope, tie up solid, sterilizing immediately;
(5) sterilizing: under 0.15MPa, 3h, normal pressure 24h carries out moist heat sterilization;
(6) inoculation: will
mushroom strainbag is put into transfer room, or in inoculating hood, ultraviolet lamp disinfection sterilization, inoculation cultivation
mushroom strain, inoculum concentration is 15 bags of/bottle of original seeds;
(7) send out bacterium: inoculate completely, cultivated species is put into ' # ' character code buttress under the condition of 25 ℃ of left and right and sends out a bacterium; After 45d cultivates, mycelium covers with cultivation bag.Wherein the described inoculation of step (6) also can be made a call to 3 caves in every bag of front, staggers and make a call to 2 caves in the back side, and cave is dark≤1.0cm, footpath, cave 2.0cm, inoculation rapidly, and seal cave with 3.5 * 3.5cm adhesive tape, every bottle of original seed can connect 100 caves, approximately 20 bacterium rods.
Embodiment 3
(1) formula: cotton seed hull 40kg, wood chip 40kg, wheat bran 15kg, corn flour 5kg, superphosphate 0.5kg, sugared 0.5kg, water 58%(w/w), material is 1:1.0 with water ratio.
(2) spice: cotton seed hull, wood chip and wheat bran and corn flour are first mixed; Separately superphosphate, sugar are stirred, then with agitator, the two stirred in water;
(3) pack: by the composts or fertilisers of cultivating of mixing, pack polypropylene plastics pocket into, bacterium bag specification: 17 * 35 * 0.045cm, installs material at 7h;
(4) sealing: first a resistant to elevated temperatures plastics neck ring is mounted on plastic bag mouth, turnup sack, and by tampon on neck stopper, then tampon is wrapped with the high pressure resistant plastic film of a fritter, with cotton rope, tie up solid, sterilizing immediately;
(5) sterilizing: under 70.15MPa, 2.5h, normal pressure 20h carries out moist heat sterilization;
(6) inoculation: will
mushroom strainbag is put into transfer room or inoculating hood, ultraviolet lamp disinfection sterilization, inoculation cultivation
mushroom strain, inoculum concentration is 15 bags of/bottle of original seeds;
(7) send out bacterium: inoculate completely, cultivated species is put into ' # ' character code buttress under the condition of 25 ℃ of left and right and sends out a bacterium; After 40d cultivates, mycelium covers with cultivation bag.Wherein the described inoculation of step (6) also can be made a call to 3 caves in every bag of front, staggers and make a call to 2 caves in the back side, and cave is dark≤1.0cm, footpath, cave 1.5cm, inoculation rapidly, and seal cave with 3.5 * 3.5cm adhesive tape, every bottle of original seed can connect 90 caves, approximately 20 bacterium rods.
Claims (2)
1. an implantation methods for mushroom, is characterized in that being undertaken by following step:
(1) formula: cotton seed hull 40kg, wood chip 40kg, wheat bran 15kg, corn flour 5kg, superphosphate 0.5kg, sugared 0.5kg, water 55%～59%(w/w), material is 1:0.9～1.1 with water ratio;
(2) spice: cotton seed hull, wood chip and wheat bran and corn flour are first mixed; Separately superphosphate, sugar are stirred, then with agitator, the two stirred in water;
(3) pack: by the composts or fertilisers of cultivating of mixing, pack polypropylene plastics pocket into, bacterium bag specification: 17 * 35 * 0.045cm, installs material at 6h～8h;
(4) sealing: first a resistant to elevated temperatures plastics neck ring is mounted on plastic bag mouth, turnup sack, and by tampon on neck stopper, then tampon is wrapped with the high pressure resistant plastic film of a fritter, with cotton rope, tie up solid, sterilizing immediately;
(5) sterilizing: under 0.14～0.15MPa, 2h～3h, normal pressure 16h～24h carries out moist heat sterilization;
(6) inoculation: strain bag is put into transfer room, or in inoculating hood, ultraviolet lamp disinfection sterilization, inoculation mushroom culture bacterial classification, inoculum concentration is 15 bags of/bottle of original seeds;
(7) send out bacterium: inoculate completely, cultivated species is put into ' # ' character code buttress under the condition of 25 ℃ of left and right and sends out a bacterium; After 30d～45d cultivates, mycelium covers with cultivation bag.
2. the implantation methods of mushroom described in claim 1, wherein the described inoculation of step (6) also can be made a call to 3 caves in every bag of front, stagger and make a call to 2 caves in the back side, cave is dark≤1.0cm, footpath, cave 1.5～2.0cm, inoculation rapidly, and seal cave with 3.5 * 3.5cm adhesive tape, every bottle of original seed can connect 80～100 caves, 20 bacterium rods.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488558A (en) * | 2014-12-31 | 2015-04-08 | 天津市金三农农业科技开发有限公司 | Method for increasing spawn running rate of mushroom |
| CN104737804A (en) * | 2015-03-19 | 2015-07-01 | 邬方成 | Shiitake mushroom cultivation method |
| CN104798601A (en) * | 2015-05-13 | 2015-07-29 | 张家港市鸿嘉数字科技有限公司 | Cultivation method for lentinula edodes |
| CN105330374A (en) * | 2015-11-30 | 2016-02-17 | 铜仁市万山区海洋农业科技发展有限公司 | Lentinus edodes culture medium and preparation method thereof |
| CN107306669A (en) * | 2017-08-15 | 2017-11-03 | 贵州天圣绿野生物科技有限公司 | A kind of mushroom mating system |
| CN107318464A (en) * | 2017-07-28 | 2017-11-07 | 安徽丰尚生态农业科技有限公司 | A kind of drought-enduring implantation methods of the high yield of mushroom |
| CN107493957A (en) * | 2017-07-19 | 2017-12-22 | 林燕 | A kind of implantation methods of mushroom |
| CN109275492A (en) * | 2018-06-14 | 2019-01-29 | 陕西蓝米信息技术有限公司 | A kind of mushroom implantation methods |
| CN111972215A (en) * | 2020-08-28 | 2020-11-24 | 衡阳市长航菌业有限责任公司 | Mushroom culture medium and preparation method thereof |
-
2013
- 2013-12-31 CN CN201310749291.XA patent/CN103749152A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488558A (en) * | 2014-12-31 | 2015-04-08 | 天津市金三农农业科技开发有限公司 | Method for increasing spawn running rate of mushroom |
| CN104737804A (en) * | 2015-03-19 | 2015-07-01 | 邬方成 | Shiitake mushroom cultivation method |
| CN104798601A (en) * | 2015-05-13 | 2015-07-29 | 张家港市鸿嘉数字科技有限公司 | Cultivation method for lentinula edodes |
| CN105330374A (en) * | 2015-11-30 | 2016-02-17 | 铜仁市万山区海洋农业科技发展有限公司 | Lentinus edodes culture medium and preparation method thereof |
| CN107493957A (en) * | 2017-07-19 | 2017-12-22 | 林燕 | A kind of implantation methods of mushroom |
| CN107318464A (en) * | 2017-07-28 | 2017-11-07 | 安徽丰尚生态农业科技有限公司 | A kind of drought-enduring implantation methods of the high yield of mushroom |
| CN107306669A (en) * | 2017-08-15 | 2017-11-03 | 贵州天圣绿野生物科技有限公司 | A kind of mushroom mating system |
| CN109275492A (en) * | 2018-06-14 | 2019-01-29 | 陕西蓝米信息技术有限公司 | A kind of mushroom implantation methods |
| CN111972215A (en) * | 2020-08-28 | 2020-11-24 | 衡阳市长航菌业有限责任公司 | Mushroom culture medium and preparation method thereof |
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