CN103627649A - Bacillus subtilis fermentation medium - Google Patents
Bacillus subtilis fermentation medium Download PDFInfo
- Publication number
- CN103627649A CN103627649A CN201310189938.8A CN201310189938A CN103627649A CN 103627649 A CN103627649 A CN 103627649A CN 201310189938 A CN201310189938 A CN 201310189938A CN 103627649 A CN103627649 A CN 103627649A
- Authority
- CN
- China
- Prior art keywords
- parts
- bacillus subtilis
- water
- radix astragali
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A disclosed bacillus subtilis fermentation medium is composed of the following components in parts by weight: 2-10 parts of wheat bran, 1-8 parts of corn flour, 1-6 parts of lactose, 0.01-0.5 part of beef extract, 0.01-0.4 part of ammonium sulfate, 0.01-0.5 part of sodium hydroxide, 20-40 parts of radix astragali aqueous solution, 1-10 parts of haw aqueous solution and 50-74 parts of double-distilled water. According to the bacillus subtilis fermentation medium provided by the inveiton, wheat bran, corn flour, lactose, beef extract, ammonium sulfate, sodium hydroxide, radix astragali aqueous solution and haw aqueous solution are compounded for preparing the fermentation medium, the good growth of bacillus subtilis is improved and the spore generating rate of bacillus subtilis is improved; and also a bacillus subtilis liquid fermented by the medium has the effect of improving immunity and promoting feed intake.
Description
Technical field
The invention belongs to microbial fermentation technology field, relate to a kind of fermentation of bacillus subtilis substratum.
Background technology
Subtilis is a kind of of bacillus, belongs to gram-positive microorganism, and without pod membrane, peritrichous, can move.Subtilis is as probiotic bacterium, have advantages of as follows: in thalli growth process, produce subtilyne, polymyxin, nystatin, linear gramicidins isoreactivity material, these active substances have obvious restraining effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Can consume rapidly the free oxygen in environment, cause enteron aisle hypoxemia, promote useful anerobe growth, and produce organic acid, reduce enteron aisle pH value, indirectly suppress other pathogenic bacterium growth; Growing of stimulating animal immune organ, activates T, bone-marrow-derived lymphocyte, improves immunoglobulin (Ig) and antibody horizontal, strengthens cellular immunization and humoral immune function, improves herd immunity; Thalline self can synthesize the enzyme such as α-amylase, proteolytic enzyme, lipase, cellulase element, in digestive tube with animal body in digestive ferment together play a role; The multiple vitamin B group such as energy synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising animal body internal interference element and scavenger cell.Subtilis, as useful microorganism, has been obtained good effect at aspects such as purifying aquatic product cultivating environment, peaceful control of crop disease, additive for farm animal feed at present, more and more gets more and more people's extensive concerning in recent years.
But in current Industrial processes, there is the problem that spore forming rate is not high, production cost is too high in bacillus subtilis microbial agent; In microbial inoculum, gemma rate is low, there is no the thalline death at short notice of gemma, greatly reduces activity and the quality stability of microbial inoculum.
Summary of the invention
The object of this invention is to provide a kind of fermentation of bacillus subtilis substratum, can promote the good growth of subtilis, improve the product gemma rate of subtilis.
In order to realize above object, the technical solution adopted in the present invention is: a kind of fermentation of bacillus subtilis substratum, is comprised of the component of following parts by weight: 2～10 parts, wheat bran, 1～8 part of Semen Maydis powder, 1～6 part of lactose, 0.01～0.5 part of extractum carnis, 0.01～0.4 part, ammonium sulfate, 0.01～0.5 part, sodium hydroxide, 20～40 parts, Radix Astragali water, 1～10 part, hawthorn water, 50～74 parts of distilled waters.
Above-mentioned fermentation of bacillus subtilis substratum, preferably, the component by following parts by weight forms: 6 parts, wheat bran, 2 parts of Semen Maydis powder, 2 parts of lactose, 0.08 part of extractum carnis, 0.02 part, ammonium sulfate, 0.03 part, sodium hydroxide, 40 parts, Radix Astragali water, 4 parts, hawthorn water, 64 parts of distilled waters.
Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described Radix Astragali water can also be commercial goods.
Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water can also be commercial goods.
Described distilled water can be commercial goods.
Fermentation of bacillus subtilis substratum of the present invention, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution high pressure (1.05kg/cm under 121 ℃ of conditions
2) sterilizing 15min, obtain.
Fermentation of bacillus subtilis substratum of the present invention, the property of medicine drug effect that relates to traditional Chinese medicine ingredients is as follows:
The Radix Astragali: property is sweet, warm; Return lung, the spleen channel.There is the immunity system of enhancing, delay senility.Anti-oxidant, strengthen the effects such as cardiovascular systems, antithrombotic, antiviral, anti-inflammatory and analgesia; Benefiting qi and raising yang, strengthening superficial resistance to stop perspiration, inducing diuresis to remove edema, promoting pus discharge and tissue regeneration by strengthening QI; Cure mainly the card of all Qi and blood deficiencies, as diarrhea due to hypofunction of the spleen, cough due to deficiency of the lung, prolapse of the anus, uterine prolapse, spontaneous perspiration, night sweat, oedema, blood-arthralgia, ulcer is difficult bursts or bursts for a long time and do not hold back etc.
Hawthorn: property acid, sweet, tepor; Return spleen, stomach, Liver Channel.There is digestion promoting, step-down, lipopenicillinase, the effect such as anti-oxidant; Food digesting stomach fortifying, promoting the circulation of qi scattered silt; Cure mainly eating accumulation, abdominal distention, dysentery etc.
Fermentation of bacillus subtilis substratum of the present invention, wherein, wheat bran contains sporeformer grow needed vitamin B group, amino acid, trace element; Semen Maydis powder contains the sporeformer trace elements such as needed vitamin-E, calcium, phosphorus, potassium, sodium, magnesium, iron, zinc, selenium, copper, manganese of growing; Lactose provides carbon source and the energy for bacillus subtilis bacteria growing; Extractum carnis provides nitrogenous source for bacillus subtilis bacteria growing; Ammonium sulfate provides nitrogenous source for bacillus subtilis bacteria growing; Sodium hydroxide is for regulating pH value at bacillus subtilis bacterium culture medium.
Gemma is bacteriotrophy body when must nutriment being about to exhaust, the hypopus that the water content forming in cell is extremely low, resistance is extremely strong.The gemma of subtilis is ellipse or column, be positioned at thalline central authorities or slightly off normal put.The gemma of subtilis is easily preserved compared with its nourishing body cell, and resurrection rate is high, is the desirable existence form of preparing bacillus subtilis formulation; The multiple resistance such as that the gemma of subtilis has is heat-resisting, drought-enduring, uvioresistant and organic solvent, produce in the process of machine high temperature drying at interchangeable manufacturing, can guarantee the activity of microbiobacterial agent to improve its quality product.
Fermentation of bacillus subtilis substratum of the present invention, adopt wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide and Radix Astragali water, the composite fermention medium of making of hawthorn water, can promote the good growth of subtilis, improve the product gemma rate of subtilis; Use the bacillus subtilis microbial agent of this culture medium culturing to there is higher gemma rate, guaranteed the activity of microbial inoculum, improved quality and the quality stability of product; Meanwhile, use the bacillus subtilis microbial agent of this culture medium culturing to there is the effect that improves immunizing power and promote food consumption.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 2 parts, wheat bran, 8 parts of Semen Maydis powder, 1 part of lactose, 0.5 part of extractum carnis, 0.2 part, ammonium sulfate, 0.01 part, sodium hydroxide, 40 parts, Radix Astragali water, 1 part, hawthorn water, 50 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Embodiment 2
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 10 parts, wheat bran, 5 parts of Semen Maydis powder, 4 parts of lactose, 0.01 part of extractum carnis, 0.4 part, ammonium sulfate, 0.3 part, sodium hydroxide, 30 parts, Radix Astragali water, 10 parts, hawthorn water, 62 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Embodiment 3
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 8 parts, wheat bran, 1 part of Semen Maydis powder, 6 parts of lactose, 0.2 part of extractum carnis, 0.01 part, ammonium sulfate, 0.5 part, sodium hydroxide, 20 parts, Radix Astragali water, 6 parts, hawthorn water, 74 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Embodiment 4
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 6 parts, wheat bran, 2 parts of Semen Maydis powder, 2 parts of lactose, 0.08 part of extractum carnis, 0.02 part, ammonium sulfate, 0.03 part, sodium hydroxide, 40 parts, Radix Astragali water, 4 parts, hawthorn water, 65 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Experimental example
This experimental example is used the fermentation of bacillus subtilis substratum of embodiment 1～4 to cultivate subtilis, and its result is as shown in table 1.
Table 1 subtilis culture experiment result
| Substratum | Incubation time/h | Produce gemma rate/% |
| Embodiment 1 | 36 | 92 |
| Embodiment 2 | 36 | 92 |
| Embodiment 3 | 36 | 93 |
| Embodiment 4 | 36 | 95 |
| Comparative example | 36 | 70 |
Wherein, comparative example Media Components is: glucose 2 weight parts, peptone 1.5 weight parts, sodium-chlor 0.5 weight part, extractum carnis 0.05 weight part, distilled water 100 weight parts.
Claims (4)
1. a fermentation of bacillus subtilis substratum, is characterized in that: the component by following parts by weight forms: 2～10 parts, wheat bran, 1～8 part of Semen Maydis powder, 1～6 part of lactose, 0.01～0.5 part of extractum carnis, 0.01～0.4 part, ammonium sulfate, 0.01～0.5 part, sodium hydroxide, 20～40 parts, Radix Astragali water, 1～10 part, hawthorn water, 50～74 parts of distilled waters.
2. fermentation of bacillus subtilis substratum according to claim 1, is characterized in that: the component by following parts by weight forms: 6 parts, wheat bran, 2 parts of Semen Maydis powder, 2 parts of lactose, 0.08 part of extractum carnis, 0.02 part, ammonium sulfate, 0.03 part, sodium hydroxide, 40 parts, Radix Astragali water, 4 parts, hawthorn water, 64 parts of distilled waters.
3. fermentation of bacillus subtilis substratum according to claim 1 and 2, is characterized in that: described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
4. fermentation of bacillus subtilis substratum according to claim 1 and 2, is characterized in that: described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310189938.8A CN103627649B (en) | 2013-05-21 | 2013-05-21 | Bacillus subtilis fermentation medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310189938.8A CN103627649B (en) | 2013-05-21 | 2013-05-21 | Bacillus subtilis fermentation medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103627649A true CN103627649A (en) | 2014-03-12 |
| CN103627649B CN103627649B (en) | 2015-05-20 |
Family
ID=50209101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310189938.8A Expired - Fee Related CN103627649B (en) | 2013-05-21 | 2013-05-21 | Bacillus subtilis fermentation medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103627649B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293690A (en) * | 2014-05-27 | 2015-01-21 | 河南牧业经济学院 | Traditional Chinese medicinal micro-ecological composition, preparation thereof, and preparation method of preparation |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
| CN106544304A (en) * | 2016-12-08 | 2017-03-29 | 青岛益佰农肥业有限公司 | With fixed nitrogen and the bacillus subtilis formulation for preventing and treating soil-borne disease dual-use function and preparation method thereof |
| CN108703253A (en) * | 2018-04-09 | 2018-10-26 | 华中农业大学 | A kind of feeding natural plants fermentation preparation and its preparation method and application preventing livestock and poultry |
| CN113201477A (en) * | 2021-05-10 | 2021-08-03 | 江苏绿科生物技术有限公司 | Production process of hay compound culture medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
| CN101244184A (en) * | 2007-04-11 | 2008-08-20 | 北京龙科方舟生物工程技术中心 | Enteron modifying agent, preparation method and application thereof |
| CN102366024A (en) * | 2011-09-16 | 2012-03-07 | 汪松林 | Production method of pig feed additive containing traditional Chinese medicine and active probiotics |
-
2013
- 2013-05-21 CN CN201310189938.8A patent/CN103627649B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
| CN101244184A (en) * | 2007-04-11 | 2008-08-20 | 北京龙科方舟生物工程技术中心 | Enteron modifying agent, preparation method and application thereof |
| CN102366024A (en) * | 2011-09-16 | 2012-03-07 | 汪松林 | Production method of pig feed additive containing traditional Chinese medicine and active probiotics |
Non-Patent Citations (3)
| Title |
|---|
| 文宇婷，等: "黄芪多糖-枯草芽孢杆菌合生元菌液的研制", 《沈阳农业大学学报》, vol. 43, no. 1, 15 February 2002 (2002-02-15), pages 98 - 101 * |
| 王振华，潘康成: "不同浓度中药对枯草芽孢杆菌体外生长的影响", 《中国兽医杂志》, vol. 43, no. 10, 22 October 2007 (2007-10-22), pages 44 - 45 * |
| 王永芬 等: "不同生物制剂对猪致病性大肠杆菌的体外抑菌试验", 《云南农业大学学报》, vol. 27, no. 1, 13 January 2012 (2012-01-13), pages 60 - 66 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293690A (en) * | 2014-05-27 | 2015-01-21 | 河南牧业经济学院 | Traditional Chinese medicinal micro-ecological composition, preparation thereof, and preparation method of preparation |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
| CN106544304A (en) * | 2016-12-08 | 2017-03-29 | 青岛益佰农肥业有限公司 | With fixed nitrogen and the bacillus subtilis formulation for preventing and treating soil-borne disease dual-use function and preparation method thereof |
| CN108703253A (en) * | 2018-04-09 | 2018-10-26 | 华中农业大学 | A kind of feeding natural plants fermentation preparation and its preparation method and application preventing livestock and poultry |
| CN113201477A (en) * | 2021-05-10 | 2021-08-03 | 江苏绿科生物技术有限公司 | Production process of hay compound culture medium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103627649B (en) | 2015-05-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102366023B (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN102366024B (en) | Production method of pig feed additive containing traditional Chinese medicine and active probiotics | |
| CN101919494B (en) | Complex microorganism additive as well as preparation method and application thereof | |
| CN103627656B (en) | A kind of clostridium butyricum and bacillus coagulans mixed culture solid state fermentation method | |
| CN106974063B (en) | Method for producing high-efficiency protein feed by using feed enzyme in cooperation with bacillus coagulans | |
| CN103798540A (en) | Multi-strain microorganism pig feed additive and production method thereof | |
| CN109593666B (en) | Composite microecological preparation and preparation method and application thereof | |
| CN105685475A (en) | Method for preparing feed antibiotic substitute from subtilin producing bacillus subtilis fermented Chinese herbal medicine | |
| CN103627649B (en) | Bacillus subtilis fermentation medium | |
| CN105432935A (en) | Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry | |
| CN105746881A (en) | Method for preparing substituent of antibiotics for feed by fermenting Chinese herbal medicines through lipopeptide-producing bacillus subtilis | |
| CN102559560B (en) | Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis | |
| CN103289910A (en) | Solid fermentation production method of bacillus coagulans | |
| CN102367418B (en) | Traditional Chinese medicine probiotics liquid medium used for pig feed, culturing method and feed additive | |
| CN103865854A (en) | Composite micro-ecologic preparation and preparation method thereof | |
| CN105994941A (en) | Non-antibiotic feed prepared through microbial fermentation | |
| CN102367416B (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
| CN103184174A (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN101836688B (en) | Preparation method of antibiotic-free microbial fermentation feed | |
| CN101857469A (en) | Preparation process for golf course lawn slow-release bio-organic fertilizer produced by three continuous fermentations | |
| CN103263444B (en) | Micro-ecological compound preparation for preventing and treating stress diarrhoea of goat and preparation method thereof | |
| CN103289935A (en) | Compound strain microecological preparation and preparation method thereof | |
| CN102860411A (en) | Lactobacillus rhamnosus probiotic and preparation method thereof | |
| CN105941835A (en) | Method for preparing bacillus subtilis traditional Chinese medicine feed additive | |
| CN104082528B (en) | A kind of feed addictive and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150520 Termination date: 20160521 |