CN103604934B - Domestic animal chorionic-gonadotropin hormone method for quick and quickly detection test card - Google Patents
Domestic animal chorionic-gonadotropin hormone method for quick and quickly detection test card Download PDFInfo
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- CN103604934B CN103604934B CN201310616079.6A CN201310616079A CN103604934B CN 103604934 B CN103604934 B CN 103604934B CN 201310616079 A CN201310616079 A CN 201310616079A CN 103604934 B CN103604934 B CN 103604934B
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/575—Hormones
- G01N2333/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention is a breeding class chorionic-gonadotropin hormone method for quick and quickly detects test card, this detection test card is made up of testing cassete and test strips structure, testing cassete includes lid (1) and cassette bottom (2), described lid (1) center is provided with observation window (3), and test strips structure is arranged on cassette bottom (2) inner surface and is in observation window (3) lower section；Cassette bottom (2) bottom end is provided with opening (18) and inhales sample block (9), and described suction sample block (9) is inverted trapezoidal, is plugged in described opening (18), and its long bottom is in box, and short bottom is outside box.The present invention directly draws domestic animal and comes the urine on ground and immediately detect, and is not affected by other dirt of ground, need not manually and special installation goes to receive urinating of domestic animal；Detection equipment precise and high efficiency, be easy to carry, simple and efficient, instant, low cost, easily operated；The quantity of pregnant tire can be gone out according to the different change-detection of the chorionic-gonadotropin hormone content of different domestic animal.
Description
Technical field
The invention belongs to animal and veterinary field, especially relate to a kind of immunology detection technology, be that poultry disease is the most anti-
Control, the maximally effective device and method of effectively breeding detection monitoring.
Background technology
Set up quick, accurate, simple and easy to do early stage female animal gestation Fast Detection Technique, be engaged in Field of Animal Epidemic Disease Control,
The research workers such as animal reproduction and the eager desire of industrial circle personage.
Early pregnancy detection is an important technology, significant in China's aquaculture produces.Gestation female animal
Class pregnant detecting method in early days, through clinical practice experience for many years and the development of zoology science and technology, existing various clinical method, with sow
As a example by include: pregnant mare serum gonadotrop(h)in (PMSG) (PMSG) method of hormone method and diethylstilbestrol method.In the urine of uroscopy methodEstrone
Method of diagnosis, liquid iodate inspection technique；Ultrasound be suitable for B-mode instrument, Doppler ultrasonic diagnosis apparatus (D type, A type
Diasonograph is used.And the boar examination feelings method of other method, examination per vagina method.
In addition to the above methods, also blood or RuzhongProgesterone measuresMethod, EPF detection method,Red cell agglutinationMethod, pinch pressure lower back
Method andCervix uteriMucus plate coating checking etc..Sow diagnosis of early gestation method has a lot, and they cut both ways, and during clinical practice are
Select according to practical situation.These methods, are not that accuracy rate is relatively low, it is simply that use cost is high, and can not immediately monitoring, to going out
Some existing female animals nonpregnant several feelings phases and do not return feelings, and be delayed in time breeding, make the breeding cycle extend, reproductive-cost increases,
Thus reduce Swine Production benefit.
Since opening up immuno analytical method, more and more sensitiveer, more easily immune analysis method be developed
Come, the quick detection test paper set up based on colloidal gold immunochromatographimethod (dual-antigen sandwich method, double antibody sandwich method) method,
It it is considered as sensitive a kind of immunology detection technology the most quick, high.This technology is truly realized fast simple detection
Target, and be generalizable in animal husbandry, become the prevention and control in early days of current poultry disease, effectively breeding detection monitoring
One of most effectual way.
Amynologic diagnostic method not only recall rate is high, and reacts quickly, has obtained in medical science, veterinary and Aquatic product field
Extensively application.Traditional classical amynologic diagnostic method mainly includes immunoprecipitation (immunodiffusion, immunoelectrophoresis etc.), exempts from
Epidemic disease coagulation etc., but all can not realize efficient, quick, easy, instant early stage detection.
The party's science of law, it is possible to high efficiency, healthy breeding for aquaculture provide timely foundation and effective information, during reduction
Between, the feeding cost such as feedstuff；Meanwhile, can be greatly improved, because it is easy to carry, the controllability commonly raising user scattered to pregnant domestic animal, fall
The low unnecessary property loss just before giving birth caused because of field.
At present, domestic animal early pregnancy detection in early days, the concrete grammar commonly used strip method of monitoring carry out urine analysis, by strip
Insert in the urine in container, easy to use, however it is necessary that artificial collection domestic animal animals urine, need to follow domestic animal for a long time and move
Thing is also ready to gather urine, extremely inconvenience.The early pregnancy rapid test piece of existing Patent No. ZL200420077747.9 is sent out
Bright patent, hole insertion urine (or titer, contrasting fluid, test fluid) constituted by groove can carry out taking liquid and detect, and domestic animal
Discharge urine is directly discharged on the ground, and the hole that groove is constituted should not be inserted directly into discharges urine on the ground and carry out taking liquid inspection
Survey.
Therefore, be directed to the cultivated animals of economic class, there is no so far a kind of be really achieved efficient, quick, easy, instant
The most quickly detection test card and method for quick.
Summary of the invention
The domestic animal chorionic-gonadotropin hormone that the purpose of the present invention is to propose to a kind of mammal special quickly detects survey
Examination card and method for quick, by the index of (referring to pregnant domestic animal) chorionic-gonadotropin hormone in domestic animal urine detection body, sentence more
Disconnected pregnancy of being the most fertilized, the early diagnosis for the effectively breeding of cultivated animals is offered reference.
In order to realize object of the invention it is proposed techniques below scheme:
One breeding class chorionic-gonadotropin hormone quickly detects test card, and described detection test card is by testing cassete and test strips
Structure is constituted, and described testing cassete includes lid 1 and cassette bottom 2, together with described lid 1 is identical with cassette bottom 2 grafting；
Described lid 1 center is provided with observation window 3, and test strips structure is arranged on cassette bottom 2 inner surface and is in observation window 3 times
Side；
Described cassette bottom 2 bottom end is provided with opening 18 and inhales sample block 9, and described suction sample block 9 is inverted trapezoidal, be plugged on described in open
In mouth 18, its long bottom is in box, and short bottom is outside box；Described test strips structure include base plate 11, absorbent paper 10, NC film 12,
Rubber cover 13 and 5 colloidal gold pad 14；Described base plate 11 is fixed on bottom cassette bottom 2, described absorbent paper 10, NC film 12 and colloidal gold pad
14 sequentially overlap and are pasted onto the upper surface of base plate 11, and overlapping mode is absorbent paper 10 and colloidal gold pad 14 covers NC film 12 respectively
Two ends；The other end of described colloidal gold pad 14 is completely covered described suction sample block 9 upper surface, and described NC film 12 is in observation window 3
Underface, described rubber cover 13 covers in NC film 12 and the lap-joint of colloidal gold pad 14.
Described lid 1 inner surface is provided with fixing post 6, gim peg 4, slide 15 and pressure gold stand 5, and described fixing post 6 is respectively
It is arranged at four angles of rectangle；Described slide 15 covers observation window 3；Described gim peg 4 and pressure gold stand 5 are separately positioned on sight
Examining the both sides of window 3, wherein gim peg 4 is in above absorbent paper 10, and pressure gold stand 5 is in above suction sample block 9；
Described cassette bottom 2 inner surface is provided with fixing post holes 7, and the position of described fixing post holes 7 is corresponding with described fixing post 6, institute
State lid 1 with cassette bottom 2 by fixing post 6 and fixing post holes 7 grafting；
When described lid 1 fastens with cassette bottom 2, described pressure gold stand 5 coordinates suction sample block 9 will cover at the glue inhaled on sample block 9
One side pressure of body gold pad 14 and rubber cover 13 is steady；Absorbent paper 10 is fixed by described gim peg 4.
Described cassette bottom 2 outer surface is provided with outer pillar 8, and inner surface is provided with desiccant storeroom 16 and clip slot shelves 17, described outside
Pillar 8 and desiccant storeroom 16 are in the opposite side of opening 18, and described desiccant storeroom 16 is arranged on two fixing posts
In the middle of hole 7, described clip slot shelves 17 surround a rectangle frame, are in 4 summits of fixing post holes 7, and described base plate 11 is fixing should
In rectangle frame.
The present invention also proposes another kind of technical scheme, is respectively provided with and closes at wherein said lid 1 and cassette bottom 2 side fastening
Zygostyle 21 and lower Guan Bi post 22, arrange pressure gold stand 19 down in this side cassette bottom 2 with pressure gold stand 5 correspondence position, and lid 1 and cassette bottom 2 are detained
During conjunction, lower pressure gold stand 19 is respectively closed with pressure gold stand 5, upper Guan Bi post 21 and lower Guan Bi post 22；
Described suction sample block (9) lengthening broadening is rectangular lamellar, is arranged on closing of Guan Bi post (21) and lower Guan Bi post (22)
At conjunction and press down gold stand (19) and press at the Guan Bi of gold stand (5), and stretch out outside box；
Described upper Guan Bi post (21) and lower Guan Bi post (22) Guan Bi clamp suction sample block (9), coordinate pressure with pressure gold stand 19 at present
Suction sample block 9, colloidal gold pad 14 and rubber cover 13 are pressed surely by gold stand 5.
The present invention also proposes yet another technique scheme, wherein, the cassette bottom 2 below described suction sample block 9 is arranged down and presses gold stand
19；Arranging well 20 in inhaling the lid 1 above sample block 9, when lid 1 fastens with cassette bottom 2, lower pressure gold stand 19 coordinates well
20 will inhale a side pressure of sample block 9, colloidal gold pad 14 and rubber cover 13 surely, inhale sample block 9 and expose from lid 1.
The present invention proposes to use the detection method of above-mentioned domestic animal chorionic-gonadotropin hormone detection test card, described detection side
Method includes step:
1) lid 1 is fastened with cassette bottom 2, make described pressure gold stand 5 coordinate suction sample block 9 will cover at the colloid inhaled on sample block 9
One side pressure of gold pad 14 and rubber cover 13 is steady, and absorbent paper 10 is fixed by gim peg 4, and absorbent paper 10 and colloidal gold pad 14 are covered respectively
Live the two ends of NC film 12；
2) box body is directly kept flat to domestic animal animals urine, make suction sample block 9 contact and absorb animals urine, filter it miscellaneous
Matter, keeps 5～10 seconds；
3) taking-up keeps flat, and observes test result from observation window；
4) when reagent paper NC film 12, two aubergines or red stripes occur, positive for cyesiognosis；Only occur in control zone
Article one, aubergine band red stripes, negative for cyesiognosis；When p-wire and control line do not develop the color, represent test card nothing
Effect.
Using the detection method of another kind of domestic animal chorionic-gonadotropin hormone detection test card, described detection method includes step
Rapid:
1) lid 1 is fastened with cassette bottom 2, make described pressure gold stand 5 coordinate and inhale sample block 9 by colloidal gold pad 14 and rubber cover 13
One side pressure is steady, and absorbent paper 10 is fixed by gim peg 4, and absorbent paper 10 and colloidal gold pad 14 cover the two ends of NC film 12 respectively；
2) suction sample block 9 box outer portion is directly dipped domestic animal animals urine, inhale sample block 9 and absorb animals urine, and filter it
Impurity, keeps 5～10 seconds；
3) taking-up keeps flat, and observes test result from observation window；
4) when reagent paper NC film 12, two aubergines or red stripes occur, positive for cyesiognosis；Only occur in control zone
Article one, aubergine band red stripes, negative for cyesiognosis；When p-wire and control line do not develop the color, represent test card nothing
Effect.
Using the detection method of another domestic animal chorionic-gonadotropin hormone detection test card, described detection method includes step
Rapid:
1) being fastened with cassette bottom 2 by lid 1, lower pressure gold stand 19 coordinates well 20 will inhale sample block 9, colloidal gold pad 14 and cover
One side pressure of glue 13 is steady；Absorbent paper 10 is fixed by gim peg 4, and absorbent paper 10 and colloidal gold pad 14 cover the two of NC film 12 respectively
End；
2) box body is kept flat, drip domestic animal animals urine to well 20, inhale sample block 9 and absorb animals urine, and filter it
Impurity, keeps 5～10 seconds；
3) taking-up keeps flat, and observes test result from observation window；
4) when reagent paper NC film 12, two aubergines or red stripes occur, positive for cyesiognosis；Only occur in control zone
Article one, aubergine band red stripes, negative for cyesiognosis；When p-wire and control line do not develop the color, represent test card nothing
Effect.
The method of the present invention also includes that the treatment fluid before using described suction sample block 9 invades bubble, and this treatment fluid is according to the following steps
Order is prepared:
1) taking 18,000,000 ultra-pure water 4000ml, the clean beaker clean by cleaning contains；
2) 60gTris reagent (analytical pure) is claimed to be directly added into, stirring and dissolving；
3) claim 48gPVP reagent, be directly added into, dissolve；
4) claiming Casein (casein) 30g, be directly added into, stirring is completely dissolved；
5) Na is added2CO3(anhydrous) 20g, is directly added into dissolving；
6) claim S-9 (Tetronic1307) reagent, be directly added into, be completely dissolved；
7) measure 13.8mlTritonX-100 reagent, be completely dissolved；
8) add 1.0gNaN3 protein protective agent, be completely dissolved；
9) allocate with dilute HCC, until PH=9.0.
The advantage that prior art of the present invention is compared:
The present invention directly draws domestic animal and comes the urine on ground and immediately detect, and not by the shadow of other dirt of ground
Ring, need not manually and special installation goes reception the urinating of domestic animal；
Detection equipment precise and high efficiency, be easy to carry, simple and efficient, instant, low cost, easily operated；
The equipment Inspection purposes of the present invention is many.Can also be according to the different changes of the chorionic-gonadotropin hormone content of different domestic animal
Change the quantity detecting pregnant tire.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present invention；
Fig. 2 is cassette bottom inner surface schematic diagram；
Fig. 3 is another kind embodiment overall structure schematic diagram of the present invention；
Fig. 4 is another embodiment overall structure schematic diagram of the present invention.
In figure
Lid 1 NC film 12
Cassette bottom 2 rubber cover 13
Observation window 3 colloidal gold pad 14
Gim peg 4 slide 15
Pressure gold stand 5 desiccant storeroom 16
Post holes 17 fixed by fixing post 6
Fixing post holes 7 opening 18
8 times pressure gold stand 19 of outer pillar
Inhale sample block 9 well 20
Post 21 is closed in absorbent paper 10
11 times Guan Bi posts 22 of base plate
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with the drawings and specific embodiments,
The present invention is described in more detail.
Female mammals in gestation, not only its anterior pituitary secretion promoting sexual gland hormone, and the chorion of Placenta Hominis is also
Can secrete, referred to as chorionic gonadotrophin (swashing) element of placenta secretion, composition is glycoprotein, is a kind of glycoprotein hormones, by α, β two
Individual subunit is constituted, and β subunit is the major part showing its activity；There is the effect of similar metakentrin.
The present invention is to utilize immunology colloidal gold method and human chorionic gonadotropin (colloidal gold method) quick detection reagent
Know-why on the basis of development and production.
Owing to chorionic-gonadotropin hormone has high sensitivity and specificity, and become and assist diagnosis pregnant at present
Whether be pregnent internal has a conceived product, the chorionic-gonadotropin hormone of fine hair secretion be the most accurately and reliably check the most conceived
One of diagnostic method.The gestational age chorionic-gonadotropin hormone of female animal occurs in blood, and content is how many and disappearance has
Certain rule, this rule and the appearance of endometrium cup, to increase and disappear be consistent.
The present invention uses double antibody sandwich method and colloidal gold immunity chromatography principle to detect the floss in pregnant domestic animal urine qualitatively
Chorionic Gonadotropin content, is coated mouse-anti β-HCG monoclonal antibody (according to difference at the detection line on nitrocellulose filter
The coated antibody subtype of animal species is different), at control line, it is coated sheep anti-mouse igg, is equipped with gold mark mouse-anti α-HCG monoclonal anti
Body (antibody subtype according to different animals category flag is different).During detection, urine sample chromatographs under capillary effect.When domestic animal are fertilized
Time conceived, the chorionic-gonadotropin hormone antigen in urine forms promoting sexual gland hormone-gold labeling antibody with the monoclonal antibody of gold colloidal crosslinking
Complex, at the band of detection zone (T) displaing amaranth (or red), remaining part continues to migrate to be fixed with at sheep anti-mouse igg,
The band of aubergine (or red) is presented at control zone (C)；Reaction result, when there are two aubergine bands in reagent paper, for gestation
The diagnosis positive.Otherwise only at control zone (C), an aubergine band occurs, negative for cyesiognosis.When p-wire and control line
When not developing the color, represent that test card is invalid.The aubergine band presented in control zone (C) is to determine whether there is enough urine samples,
The most normal standard of chromatography process, also serves as the inner quality standard of reagent paper simultaneously.
Lutropin and follicle stimulating hormone (FSH) are all a kind of hormones secreted by antepituitary.Lutropin (LH) stimulates
Ovary is ovulated, and after follicular rupture discharges ovum, the cell in ruptured follicle continues to grow, and forms corpus luteum.Corpus luteum secretion is pregnant
Ketone, progesterone is responsible for maintaining the major hormone of gestation.If ovum fertilization, becoming great-hearted conceptus, these conceptus will be
It is combined on Uterus wall.The signal of this combination can feed back to corpus luteum, makes corpus luteum continue secretion progesterone, maintains gestation.
Lutropin antigen or antibody are easy to when finite concentration send out with antibody or the antigen of chorionic-gonadotropin hormone
Produce cross reaction.
Fig. 1 is the overall structure of the quickly detection test card of the present invention, Tu2Shi cassette bottom mechanism.As it can be seen, test card by
Testing cassete and test strips structure are constituted, and testing cassete includes lid 1 and cassette bottom 2, wherein,
Together with lid 1 coincide with cassette bottom 2 grafting, generally cuboid.Lid 1 outer surface is rectangle, and center is provided with
Observation window 3, lid 1 inner surface is sequentially provided with fixing post 6, pressure gold stand 5, slide 15 and gim peg 4, and described fixing post 6 is distributed
At four angles of lid 1, pressure gold stand 5 and gim peg 4 are separately positioned on the both sides of observation window 3, and slide 15 covers observation window 3
Interior edge.
Cassette bottom 2 outer surface is rectangle, corresponding with lid 1.It is respectively equipped with outer pillar at two angles of cassette bottom 2 outer surface side
8, cassette bottom 2 inner surface is provided with fixing post holes 7, is separately positioned on four angles of cassette bottom 2 inner surface, mutual with described 4 fixing posts 6
Grafting；The side corresponding with pressure gold stand 5 at outer surface is provided with opening 18；At opposite side, desiccant storeroom 16 is set, described dry
Drying prescription storeroom 16 is arranged in the middle of two fixing post holes 7；Between opening 18 and lamellar desiccant storeroom 16, clip slot is set
Shelves 17, described clip slot shelves 17 surround a rectangle frame with fixing post holes 7 as summit.
The test strips structure of the present invention includes base plate 11, inhales sample block 9, absorbent paper 10, NC film 12, rubber cover 13 and gold colloidal
Pad 14.Described base plate 11 is fixed in the rectangle frame that clip slot shelves 17 surround, and inhaling sample block 9 is inverted trapezoidal, is stuck at opening 18, described
Absorbent paper 10, NC film 12 and colloidal gold pad 14 sequentially overlap and are pasted onto the upper surface of base plate 11, and overlapping mode is absorbent paper 10
With the two ends that colloidal gold pad 14 covers NC film 12 respectively；The other end of the most described colloidal gold pad 14 is completely covered table on filter block 9
Face, described rubber cover 13 covers in NC film 12 and the lap-joint of colloidal gold pad 14.
When lid 1 fastens with cassette bottom 2, pressure gold stand 5 coordinates suction sample block 9 will cover in the colloidal gold pad 14 inhaled on sample block 9
Steady with a side pressure of rubber cover 13；Absorbent paper 10 is fixed by gim peg 4.
Using method: directly kept flat to domestic animal animals urine by the box body of the present invention, it is not necessary to hand-held, inhales sample block 9 and contacts
And absorb animals urine, and to filter its impurity, keep 5～10 seconds, taking-up keeps flat, and waits observed result, observes from observation window and surveys
Test result.
Desiccant in desiccant storeroom 16 is lamellar, is built in inside working of plastics, it is to avoid " the white caused after use
Pollute " and rubbish dispersion, the present invention can contact domestic animal the most in time and be emitted on ground urine, draws as tested urine sample
Inhaling on sample block 9, inhaling sample block 9 be dedicated fiber material, it is to avoid slag class material disturbs, and reacts more directly, sensitiveer, simpler and more direct, easily
In carrying, accomplish real detection at any time and monitoring；It is applicable to survey the urine of mammal, body fluid, blood.
Fig. 3 is another embodiment of the present invention overall structure schematic diagram；Former opening 18 is being closed by the present embodiment, and it sets
Put down pressure gold stand 19, be pressure gold stand 5 above lower pressure gold stand 19, be respectively provided with at lid 1 in this side and cassette bottom 2 two ends fastening
Guan Bi post 21 and lower Guan Bi post 22.When lid 1 and cassette bottom 2 fasten, lower pressure gold stand 19 with press gold stand 5, upper Guan Bi post 21 and under close
Zygostyle 22 is respectively closed.
Inhaling sample block 9 lengthening broadening is rectangular lamellar, be arranged on Guan Bi post 21 and dry lower Guan Bi post 22 and under press gold stand
19 with the Guan Bi of pressure gold stand 5 at, upper Guan Bi post 21 and lower Guan Bi post 22 close and clamp, and stretch out outside box；With pressing gold stand at present
19 coordinate pressure gold stand 5 suction sample block 9, colloidal gold pad 14 and rubber cover 13 to be pressed surely.
During use, inhale sample block 9 box outer portion and directly dip domestic animal animals urine, inhale sample block 9 and absorb animals urine, and filter
Its impurity, keeps 5～10 seconds, and taking-up keeps flat, and waits observed result, observes test result from observation window.
Fig. 4 is another embodiment overall structure schematic diagram of the present invention.The most former opening of the present embodiment 18 is closed, and it sets
Put down pressure gold stand 19；Open well 20 in the position of former pressure gold stand 5 simultaneously.
When lid 1 fastens with cassette bottom 2, lower pressure gold stand 19 coordinates well 20 will inhale sample block 9, colloidal gold pad 14 and cover
One side pressure of glue 13 is steady；Absorbent paper 10 is fixed by gim peg 4.
During use, box body is kept flat, drip domestic animal animals urine to well 20, it is not necessary to hand-held, inhale sample block 9 and absorb animal
Urine, and filter its impurity, it is ensured that the accuracy of detection, keep 5～10 seconds, wait observed result, observe from observation window 3
Test result.
The suction sample block 9 of the present invention uses front treated liquid to invade bubble, and the prescription for the treatment of liquid being somebody's turn to do order according to the following steps is made
Standby:
1, taking 18,000,000 ultra-pure water 4000ml, the clean beaker clean by cleaning contains；
2,60gTris reagent (analytical pure) is claimed to be directly added into, stirring and dissolving；
3, claim 48gPVP reagent, be directly added into, dissolve；
4, claiming Casein (casein) 30g, be directly added into, stirring is completely dissolved；
5, Na is added2CO3(anhydrous) 20g, is directly added into dissolving；
6, claim S-9 (Tetronic 1307) reagent, be directly added into, be completely dissolved；
7, measure 13.8ml TritonX-100 reagent, be completely dissolved；
8,1.0g NaN is added3Protein protective agent, is completely dissolved；
9, with dilute HCC allotment until satisfactory PH=9.0.
The formula of other portion of reagent of test paper structure requires essentially identical with common process, the antibody to different genera
(or antigen) makes the appropriate adjustments.
The function of domestic animal chorionic-gonadotropin hormone (colloidal gold method) quickly detection test card:
1, by the index of (referring to pregnant domestic animal) chorionic-gonadotropin hormone in domestic animal urine detection body, judge whether to be subject to more
The time phase that essence is conceived, conceived；
2, lutropin (LH) antibody and hcg antibody generation cross reaction in domestic animal urine are utilized
Principle, judges that some domestic animal has been fertilized pregnancy the most；
3, the relative concentration of the different threshold test chorionic-gonadotropin hormones by setting monitors the number that pregnant domestic animal is pregnant
Amount, such as 2 tires, 3 tires etc.；The kind of pregnant domestic animal, build, tire number are different, and the numerical value of chorionic-gonadotropin hormone is the most different.
4 combine with detection lutropin, pregnant domestic animal fertilization, the detection of pregnancy period indices and monitoring pregnancy period situation；
5, the auxiliary judgment concrete just before giving birth date is carried out at PA by measuring the disappearance of chorionic-gonadotropin hormone, to cultivation
Industry meaning is the most great；
6, the pathological changes that we's science of law product can be also used for Clinical detection, monitoring domestic animal produce because of some diseases, auxiliary
Carry out disease detection.
Particular embodiments described above, has carried out entering the detailed of 1 step to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail bright, be it should be understood that the specific embodiment that the foregoing is only the present invention, be not limited to the present invention, all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. done, should be included in the guarantor of the present invention
Within the scope of protecting.
Claims (5)
1. a breeding class chorionic-gonadotropin hormone quickly detects test card, and described test card includes testing cassete and test strips knot
Structure, described testing cassete is made up of lid (1) and cassette bottom (2), together with lid (1) and cassette bottom (2) grafting coincide, described lid
(1) inner surface is provided with fixing post (6), gim peg (4), slide (15) and pressure gold stand (5)；Described lid (1) and cassette bottom (2) it
Between be provided with suction sample block (9), described test strips structure includes base plate (11), absorbent paper (10), NC film (12), rubber cover (13) and glue
Body gold pad (14),
It is characterized in that:
It is respectively provided with Guan Bi post (21) and lower Guan Bi post (22), on arranging at described lid (1) and cassette bottom (2) side fastening
The cassette bottom (2) of the side of Guan Bi post (21) and lower Guan Bi post (22) arranges down with pressure gold stand (5) correspondence position and presses gold stand (19)；Institute
When stating lid (1) and cassette bottom (2) fastening, press down gold stand (19) and pressure gold stand (5), upper Guan Bi post (21) and lower Guan Bi post (22) point
Do not close；Described suction sample block (9) lengthening broadening is rectangular lamellar, is arranged on Guan Bi post (21) and the Guan Bi of lower Guan Bi post (22)
Place and pressing down at gold stand (19) and the Guan Bi of pressure gold stand (5), and stretches out outside box；Described upper Guan Bi post (21) and lower Guan Bi post
(22) Guan Bi clamps suction sample block (9), meanwhile, presses down gold stand (19) to coordinate pressure gold stand (5) will inhale sample block (9), colloidal gold pad (14)
Steady with rubber cover (13) pressure.
2. a breeding class chorionic-gonadotropin hormone quickly detects test card, and described test card includes testing cassete and test strips knot
Structure, described testing cassete is made up of lid (1) and cassette bottom (2), together with lid (1) and cassette bottom (2) grafting coincide, described lid
(1) inner surface is provided with fixing post (6), gim peg (4), slide (15), is provided with suction sample between described lid (1) and cassette bottom (2)
Block (9), described test strips structure includes base plate (11), absorbent paper (10), NC film (12), rubber cover (13) and colloidal gold pad
(14),
It is characterized in that:
Arrange down in the cassette bottom (2) of described suction sample block (9) lower section and press gold stand (19)；Arrange in inhaling the lid (1) of sample block (9) top
Well (20), when lid (1) fastens with cassette bottom (2), presses down gold stand (19) to coordinate well (20) will inhale sample block (9), glue
One side pressure of body gold pad (14) and rubber cover (13) is steady.
3. using the detection method of domestic animal chorionic-gonadotropin hormone as claimed in claim 1 detection test card, its feature exists
Step is included in, described detection method:
1) lid (1) and cassette bottom (2) are fastened, make described under press gold stand (19) to coordinate pressure gold stand (5) that sample block (9), colloid will be inhaled
Gold pad (14) and rubber cover (13) pressure are steady；
Absorbent paper (10) is fixed by gim peg (4), and absorbent paper (10) and colloidal gold pad (14) cover the two of NC film (12) respectively
End；
2) sample block (9) box outer portion will be inhaled and directly dip domestic animal animals urine, and inhale sample block (9) and absorb animals urine, and filter it
Impurity, keeps 5～10 seconds；
3) taking-up keeps flat, and observes test result from observation window；
4) when reagent paper NC film (12), two aubergines or red stripes occur, positive for cyesiognosis；One is only occurred in control zone
Bar aubergine band red stripes, negative for cyesiognosis；When p-wire and control line do not develop the color, represent test card nothing
Effect.
4. using the detection method of domestic animal chorionic-gonadotropin hormone as claimed in claim 2 detection test card, its feature exists
Step is included in, described detection method:
1) lid (1) is fastened with cassette bottom (2), press down gold stand (19) to coordinate well (20) that sample block (9), colloidal gold pad will be inhaled
And a side pressure of rubber cover (13) is steady (14)；Absorbent paper (10) is fixed by gim peg (4), absorbent paper (10) and colloidal gold pad (14)
Cover the two ends of NC film (12) respectively；
2) box body is kept flat, drip domestic animal animals urine to well (20), inhale sample block (9) and absorb animals urine, and filter it
Impurity, keeps 5～10 seconds；
3) taking-up keeps flat, and observes test result from observation window；
4) when reagent paper NC film (12), two aubergines or red stripes occur, positive for cyesiognosis；One is only occurred in control zone
Bar aubergine band red stripes, negative for cyesiognosis；When p-wire and control line do not develop the color, represent test card nothing
Effect.
5. according to the detection method described in claim 3 or 4, it is characterised in that before described suction sample block (9) uses, treated liquid is invaded
Bubble, this treatment fluid order according to the following steps is prepared:
1) taking 18,000,000 ultra-pure water 4000ml, the clean beaker clean by cleaning contains；
2) 60g analytical pure Tris reagent is claimed to be directly added into, stirring and dissolving；
3) claim 48g PVP reagent, be directly added into, dissolve；
4) claiming casein Casein30g, be directly added into, stirring is completely dissolved；
5) anhydrous Na is added2CO320g, is directly added into dissolving；
6) claim Tetronic1307S-9 reagent, be directly added into, be completely dissolved；
7) measure 13.8ml TritonX-100 reagent, be completely dissolved；
8) 1.0g NaN is added3Protein protective agent, is completely dissolved；
9) with dilute HCl allocation processing liquid, until PH=9.0.
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| CN101995470A (en) * | 2009-08-31 | 2011-03-30 | 万积成 | Colloid gold immunochromatographic assay diagnosis method for early gestation of sows and detector |
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